Rh5BG013000

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000587
Physical Location & Seq
Forward (+)
977578 .. 981046
3469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG013000.1

Sequence Viewer

Length: 1536 bp
ATGGGGCGCAAAGGGAGCGGTGTTGATACAGAAATTCAGGAGAAACCAAGCACATTCTGGACACAGTGTCCCATTTGTTGGGCAAAACTTCAATACTACACGGAGTTTCTGGATCGGTTGCTCCGTTGTCAAATATGCCATAACACATTTCAAGCTCGTGAATTGTTAGAAGATGTTGATGCAGAAGCTCTCAGGAATCAATGTCCAAACCATAAAGAGCCTCCAAAACAGTGTCCACAGGACCCCCCAAACACATTCTGGACACAGTGCTCCTTTTGTTGCGCAAAATTTCAATACTACAGTGATACTCTGGGTCGATTGCTTTGTTGTCAAATATGTCAGAAAGCATTTGAGGCTCATGAATTGAAGAACGATGTTCATCCAGAATTTTTCAGGAATCAATTTTCGAATACTGTGGAGCCTCCAAAACAGGGTCCTTCAAACTTAGCCTCAGAAAGTAGTGGTGGAAGTAAAAAGGGAAATATCACAAGATTTCAAGACGGAGATGGAACCTTCAAAAGTTTGAGGAAGGGTCAATCTTCTGGTGTCGCTGAGAAGAAGAGGAATATTGGAGCTGCTGGTGTACATAAGAAAGAGGCAAGAGAAAATGTTAGTTTCCGTCTGGATAAAAATTTTCCAAACAAGAAGAGGAAAACAGAAGAATTTGAGTTAAAAGCAAAGGAAGCCACCATGCTAGATAAAGAGGAAAGCAACTTCAAAGCTGATGTCAGTTCTGCAGCAAATGTAGCCACTCTGGAGAGCATTGAGGTTCCTGATCCTCAATTTCATAAGTTTGTAGTTGATGCAGACATGTTGGCAAATCTTTGTCAAGCTAATCAAACATGGGCTCTTTATGATACAGCAGATGGCATGCCCAGATTCTATGCTATTGTCAAGAAGGTCCTCACCCCTGGATTTAAGCTGAAAGTCAGCTGGCTGGACGCGGATCCAGATGACCAAGGTGAGATTGATTGGTGTGAAAAGGAGTTGCCAGTTGCTTGCGGTAAGTTCAGACTTGGAGACTCTACAGAAATTACGGATCACCTTATGTTTTCTCATCAGATGCACGCCACAACAGGGAGTCTTACAGGCTCTGTTTTGGTATATCCTAGGAAGGGAGAAACTTGGGCCATCTACCAGAATTGGGATATTGGGTGGAGTTCTGAGCCGGAAAAGCACGTATCTTACAAATATGAATTTGTTGAAGTCTTGTCAGATTTTGTGGAAGGTGATGGAATTGGAGTTTCTTATTTAGGCAAAGTAAAAGGATTTGTTAGTTTATTTCAGCAAACCGAGCAGCATGGGTTTCAAGTACCACCTGATGAGCTTTATAGATTTTCTCATCGAATTCCCTCTTTCAAGATGACTGGTCATGAAGGATATGGTGTTCCCTCAGGATCCTTTGAGCTAGATCCTGCTTCTTTGCCCTCCAGTATTTTGATTCATGATGTACGTGATTTGGAAATCGATAACAGAAGGAAGAATATTGAACCTAGTGATTTGTCTGGTGAATTTGTAGAAAGGTTGGTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

511

Amino Acids

57.88

Weight (kDa)

5.63

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 253 - 459 3.2e-75 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 283
AccB7I CCANNNNNTGG 1 cut(s) 78
AccBSI CCGCTC 1 cut(s) 18
AccII CGCG 1 cut(s) 944
AciI CCGC 3 cut(s) 18, 944, 1002
AclWI GGATC 8 cut(s) 120, 770, 941, 954, 1047, 1392, 1405, 1406
AcsI RAATTY 8 cut(s) 33, 287, 386, 631, 662, 1196, 1347, 1511
AfaI GTAC 3 cut(s) 585, 1314, 1452
AfiI CCNNNNNNNGG 5 cut(s) 78, 431, 1077, 1115, 1144
AflIII ACRYGT 1 cut(s) 810
AhdI GACNNNNNGTC 1 cut(s) 66
AjnI CCWGG 1 cut(s) 910
AloI GAACNNNNNNTCC 2 cut(s) 1371, 1403
AluBI AGCT 9 cut(s) 155, 188, 575, 722, 833, 922, 933, 1327, 1408
AluI AGCT 9 cut(s) 155, 188, 575, 722, 833, 922, 933, 1327, 1408
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 1 cut(s) 1014
AlwI GGATC 8 cut(s) 120, 770, 941, 954, 1047, 1392, 1405, 1406
AlwNI CAGNNNCTG 1 cut(s) 1094
AoxI GGCC 1 cut(s) 1128
ApeKI GCWGC 3 cut(s) 575, 737, 1297
ApoI RAATTY 8 cut(s) 33, 287, 386, 631, 662, 1196, 1347, 1511
ArsI GACNNNNNNTTYG 2 cut(s) 711, 743
AspA2I CCTAGG 1 cut(s) 1109
AspLEI GCGC 2 cut(s) 9, 284
AspS9I GGNCC 4 cut(s) 241, 434, 901, 1128
AsuHPI GGTGA 5 cut(s) 898, 974, 1034, 1241, 1520
AsuII TTCGAA 1 cut(s) 407
AvaII GGWCC 3 cut(s) 241, 434, 901
AvrII CCTAGG 1 cut(s) 1109
AxyI CCTNAGG 1 cut(s) 1393
BamHI GGATCC 2 cut(s) 946, 1397
BanII GRGCYC 1 cut(s) 850
BauI CACGAG 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 3 cut(s) 562, 749, 1309
BccI CCATC 4 cut(s) 500, 860, 1139, 1226
BciT130I CCWGG 1 cut(s) 912
BcoDI GTCTC 1 cut(s) 1014
BfaI CTAG 4 cut(s) 695, 1110, 1409, 1494
BfmI CTRYAG 3 cut(s) 298, 735, 1026
BisI GCNGC 3 cut(s) 576, 738, 1298
BlnI CCTAGG 1 cut(s) 1109
BlsI GCNGC 3 cut(s) 577, 739, 1299
Bme1390I CCNGG 1 cut(s) 912
Bme18I GGWCC 3 cut(s) 241, 434, 901
BmeRI GACNNNNNGTC 1 cut(s) 66
BmgT120I GGNCC 4 cut(s) 241, 434, 901, 1128
BmiI GGNNCC 7 cut(s) 243, 420, 435, 511, 771, 948, 1399
BmrFI CCNGG 1 cut(s) 912
BmsI GCATC 3 cut(s) 169, 793, 1053
BpmI CTGGAG 2 cut(s) 776, 1414
Bpu14I TTCGAA 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 1467
BsaAI YACGTR 2 cut(s) 1180, 1454
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 910, 958, 1109
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 431, 1077, 1115, 1144
Bse1I ACTGG 3 cut(s) 992, 1372, 1431
Bse21I CCTNAGG 1 cut(s) 1393
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 1 cut(s) 912
BseCI ATCGAT 1 cut(s) 1467
BseDI CCNNGG 3 cut(s) 910, 958, 1109
BseGI GGATG 1 cut(s) 379
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 5 cut(s) 78, 431, 1077, 1115, 1144
BseMII CTCAG 5 cut(s) 205, 465, 543, 1155, 1407
BseNI ACTGG 3 cut(s) 992, 1372, 1431
BseXI GCAGC 3 cut(s) 562, 749, 1309
Bsh1236I CGCG 1 cut(s) 944
BshFI GGCC 1 cut(s) 1130
BshVI ATCGAT 1 cut(s) 1467
BsiHKAI GWGCWC 1 cut(s) 272
BsiSI CCGG 1 cut(s) 1169
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 5 cut(s) 78, 431, 1077, 1115, 1144
BsmAI GTCTC 1 cut(s) 1014
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 1 cut(s) 1130
Bsp119I TTCGAA 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 272, 850
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
BspACI CCGC 3 cut(s) 18, 944, 1002
BspANI GGCC 1 cut(s) 1130
BspCNI CTCAG 5 cut(s) 204, 464, 544, 1156, 1406
BspDI ATCGAT 1 cut(s) 1467
BspFNI CGCG 1 cut(s) 944
BspHI TCATGA 3 cut(s) 358, 1372, 1444
BspLI GGNNCC 7 cut(s) 243, 420, 435, 511, 771, 948, 1399
BspMAI CTGCAG 1 cut(s) 739
BspPI GGATC 8 cut(s) 120, 770, 941, 954, 1047, 1392, 1405, 1406
BspT104I TTCGAA 1 cut(s) 407
BsrBI CCGCTC 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 583
BsrI ACTGG 3 cut(s) 992, 1372, 1431
BssECI CCNNGG 3 cut(s) 910, 958, 1109
BssMI GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 2 cut(s) 958, 1109
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 912
Bst4CI ACNGT 5 cut(s) 66, 231, 267, 302, 415
Bst6I CTCTTC 2 cut(s) 554, 641
BstAUI TGTACA 1 cut(s) 583
BstBAI YACGTR 2 cut(s) 1180, 1454
BstBI TTCGAA 1 cut(s) 407
BstC8I GCNNGC 4 cut(s) 872, 935, 1000, 1068
BstDEI CTNAG 6 cut(s) 191, 445, 451, 552, 1164, 1393
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 944
BstHHI GCGC 2 cut(s) 9, 284
BstKTI GATC 6 cut(s) 115, 778, 949, 1042, 1400, 1414
BstMAI GTCTC 1 cut(s) 1014
BstMBI GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
BstMWI GCNNNNNNNGC 6 cut(s) 15, 353, 683, 746, 1174, 1294
BstNI CCWGG 1 cut(s) 912
BstNSI RCATGY 2 cut(s) 814, 874
BstSCI CCNGG 1 cut(s) 910
BstSFI CTRYAG 3 cut(s) 298, 735, 1026
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 3 cut(s) 562, 749, 1309
BstX2I RGATCY 3 cut(s) 946, 1397, 1411
BstYI RGATCY 3 cut(s) 946, 1397, 1411
Bsu15I ATCGAT 1 cut(s) 1467
Bsu36I CCTNAGG 1 cut(s) 1393
BsuRI GGCC 1 cut(s) 1130
BsuTUI ATCGAT 1 cut(s) 1467
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 4 cut(s) 71, 236, 272, 307
Cac8I GCNNGC 4 cut(s) 872, 935, 1000, 1068
CaiI CAGNNNCTG 1 cut(s) 1094
CciI TCATGA 3 cut(s) 358, 1372, 1444
CfoI GCGC 2 cut(s) 9, 284
Cfr13I GGNCC 4 cut(s) 241, 434, 901, 1128
ClaI ATCGAT 1 cut(s) 1467
CseI GACGC 1 cut(s) 950
Csp6I GTAC 3 cut(s) 584, 1313, 1451
CviAII CATG 8 cut(s) 359, 691, 811, 843, 871, 1301, 1373, 1445
CviQI GTAC 3 cut(s) 584, 1313, 1451
DdeI CTNAG 6 cut(s) 191, 445, 451, 552, 1164, 1393
DpnI GATC 6 cut(s) 114, 777, 948, 1041, 1399, 1413
DpnII GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
DriI GACNNNNNGTC 1 cut(s) 66
Eam1104I CTCTTC 2 cut(s) 554, 641
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 2 cut(s) 554, 641
Eco130I CCWWGG 2 cut(s) 958, 1109
Eco24I GRGCYC 1 cut(s) 850
Eco47I GGWCC 3 cut(s) 241, 434, 901
Eco81I CCTNAGG 1 cut(s) 1393
EcoO109I RGGNCCY 3 cut(s) 241, 434, 901
EcoRI GAATTC 1 cut(s) 1347
EcoRII CCWGG 1 cut(s) 910
EcoT14I CCWWGG 2 cut(s) 958, 1109
EcoT38I GRGCYC 1 cut(s) 850
ErhI CCWWGG 2 cut(s) 958, 1109
FaeI CATG 8 cut(s) 362, 694, 814, 846, 874, 1304, 1376, 1448
FaqI GGGAC 1 cut(s) 54
FatI CATG 8 cut(s) 358, 690, 810, 842, 870, 1300, 1372, 1444
Fnu4HI GCNGC 3 cut(s) 576, 738, 1298
FokI GGATG 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 850
Fsp4HI GCNGC 3 cut(s) 576, 738, 1298
FspBI CTAG 4 cut(s) 695, 1110, 1409, 1494
FspI TGCGCA 1 cut(s) 283
GlaI GCGC 2 cut(s) 8, 283
GluI GCNGC 3 cut(s) 576, 738, 1298
GsuI CTGGAG 2 cut(s) 776, 1414
HaeIII GGCC 1 cut(s) 1130
HapII CCGG 1 cut(s) 1169
HgaI GACGC 1 cut(s) 950
HhaI GCGC 2 cut(s) 9, 284
Hin1II CATG 8 cut(s) 362, 694, 814, 846, 874, 1304, 1376, 1448
Hin6I GCGC 2 cut(s) 7, 282
HinP1I GCGC 2 cut(s) 7, 282
HinfI GANTC 6 cut(s) 196, 397, 879, 1022, 1081, 1441
HpaII CCGG 1 cut(s) 1169
HphI GGTGA 5 cut(s) 898, 974, 1034, 1241, 1520
Hpy166II GTNNAC 2 cut(s) 236, 584
Hpy188I TCNGA 6 cut(s) 342, 454, 1013, 1062, 1165, 1216
Hpy8I GTNNAC 2 cut(s) 236, 584
HpyAV CCTTC 8 cut(s) 447, 523, 523, 892, 1108, 1220, 1370, 1470
HpyCH4III ACNGT 5 cut(s) 66, 231, 267, 302, 415
HpyCH4IV ACGT 2 cut(s) 1179, 1453
HpyCH4V TGCA 4 cut(s) 182, 737, 806, 1066
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 353, 683, 746, 1174, 1294
HpyF3I CTNAG 6 cut(s) 191, 445, 451, 552, 1164, 1393
HpySE526I ACGT 2 cut(s) 1179, 1453
Hsp92II CATG 8 cut(s) 362, 694, 814, 846, 874, 1304, 1376, 1448
HspAI GCGC 2 cut(s) 7, 282
Kzo9I GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
LmnI GCTCC 5 cut(s) 15, 126, 275, 418, 572
Lsp1109I GCAGC 3 cut(s) 562, 749, 1309
LweI GCATC 3 cut(s) 169, 793, 1053
MaeI CTAG 4 cut(s) 695, 1110, 1409, 1494
MaeII ACGT 2 cut(s) 1179, 1453
MalI GATC 6 cut(s) 114, 777, 948, 1041, 1399, 1413
MbiI CCGCTC 1 cut(s) 18
MboI GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
MboII GAAGA 8 cut(s) 182, 379, 531, 568, 571, 658, 671, 1492
MflI RGATCY 3 cut(s) 946, 1397, 1411
MhlI GDGCHC 2 cut(s) 272, 850
MlyI GAGTC 2 cut(s) 1016, 1090
MseI TTAA 2 cut(s) 671, 918
MspA1I CMGCKG 1 cut(s) 933
MspI CCGG 1 cut(s) 1169
MspR9I CCNGG 1 cut(s) 912
MvaI CCWGG 1 cut(s) 912
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 6 cut(s) 15, 353, 683, 746, 1174, 1294
NdeII GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
NlaIII CATG 8 cut(s) 362, 694, 814, 846, 874, 1304, 1376, 1448
NlaIV GGNNCC 7 cut(s) 243, 420, 435, 511, 771, 948, 1399
NsbI TGCGCA 1 cut(s) 283
NspI RCATGY 2 cut(s) 814, 874
NspV TTCGAA 1 cut(s) 407
PaeI GCATGC 1 cut(s) 874
PagI TCATGA 3 cut(s) 358, 1372, 1444
PciI ACATGT 1 cut(s) 810
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PfeI GAWTC 4 cut(s) 196, 397, 879, 1441
PflMI CCANNNNNTGG 1 cut(s) 78
PkrI GCNGC 3 cut(s) 577, 739, 1299
PleI GAGTC 2 cut(s) 1016, 1089
PpsI GAGTC 2 cut(s) 1016, 1089
Ppu21I YACGTR 2 cut(s) 1180, 1454
PpuMI RGGWCCY 3 cut(s) 241, 434, 901
PscI ACATGT 1 cut(s) 810
Psp5II RGGWCCY 3 cut(s) 241, 434, 901
Psp6I CCWGG 1 cut(s) 910
PspGI CCWGG 1 cut(s) 910
PspN4I GGNNCC 7 cut(s) 243, 420, 435, 511, 771, 948, 1399
PspPI GGNCC 4 cut(s) 241, 434, 901, 1128
PspPPI RGGWCCY 3 cut(s) 241, 434, 901
PstI CTGCAG 1 cut(s) 739
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 3 cut(s) 946, 1397, 1411
PvuII CAGCTG 1 cut(s) 933
RsaI GTAC 3 cut(s) 585, 1314, 1452
RsaNI GTAC 3 cut(s) 584, 1313, 1451
SaqAI TTAA 2 cut(s) 671, 918
SatI GCNGC 3 cut(s) 576, 738, 1298
Sau3AI GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
Sau96I GGNCC 4 cut(s) 241, 434, 901, 1128
SchI GAGTC 2 cut(s) 1016, 1090
ScrFI CCNGG 1 cut(s) 912
SduI GDGCHC 2 cut(s) 272, 850
SfaNI GCATC 3 cut(s) 169, 793, 1053
SfcI CTRYAG 3 cut(s) 298, 735, 1026
SfuI TTCGAA 1 cut(s) 407
SinI GGWCC 3 cut(s) 241, 434, 901
SphI GCATGC 1 cut(s) 874
SsiI CCGC 3 cut(s) 18, 944, 1002
SspI AATATT 2 cut(s) 568, 1486
SspMI CTAG 4 cut(s) 695, 1110, 1409, 1494
StyD4I CCNGG 1 cut(s) 910
StyI CCWWGG 2 cut(s) 958, 1109
TaaI ACNGT 5 cut(s) 66, 231, 267, 302, 415
TaiI ACGT 2 cut(s) 1182, 1456
TaqI TCGA 4 cut(s) 316, 407, 1345, 1467
TatI WGTACW 1 cut(s) 583
TfiI GAWTC 4 cut(s) 196, 397, 879, 1441
Tru1I TTAA 2 cut(s) 671, 918
Tru9I TTAA 2 cut(s) 671, 918
TscAI CASTG 4 cut(s) 71, 236, 272, 307
TseI GCWGC 3 cut(s) 575, 737, 1297
TspDTI ATGAA 6 cut(s) 368, 375, 776, 1209, 1389, 1433
TspGWI ACGGA 5 cut(s) 113, 116, 516, 608, 1052
TspRI CASTG 4 cut(s) 71, 236, 272, 307
Van91I CCANNNNNTGG 1 cut(s) 78
VpaK11BI GGWCC 3 cut(s) 241, 434, 901
XapI RAATTY 8 cut(s) 33, 287, 386, 631, 662, 1196, 1347, 1511
XceI RCATGY 2 cut(s) 814, 874
XcmI CCANNNNNNNNNTGG 2 cut(s) 54, 255
XmaJI CCTAGG 1 cut(s) 1109
XspI CTAG 4 cut(s) 695, 1110, 1409, 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.