RchiOBHm_Chr3g0453151

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000587
Physical Location & Seq
Forward (+)
3658777 .. 3662447
3671 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42026

Sequence Viewer

Length: 2262 bp
ATGGAGTGCAACAAAGATGAGGCAGAAAGGGCAATGCAACTTTCGGAGGAAAAGATCCAAAATAAGGACTTTAAAGGAGCAAAGGAGATGGCACTAAAGGCTCAACGACTCTACCCTCAGCTTGACAACATTTCTCGCTTAATTACAGTATGTGAAGTTCACTGTTCAGCAGAAAAGAAGGTAGGGGGATCAGAAATGGACTGGTATGGCATTCTTCAGATTCAGCAATTTGATGATGAAGCAACGATCAAGAAACAATATAGAAAACTTGGGCTGCTACTTCATCCCGATAAGAATAGGTTTACTGGGGCGGAGGCTGCTTTTAAGCTGATTGGGGAAGCAAACAGGGTGCTGACAGACCAAGGAAGTCGTTCTAAATATGACATCAAGTTGAGCGCTTTAACTGGTACCAAATCATTAGGTCATCAGTCATATTTTAACTTCTTTGGACCCAAACATTCCAGTGCTGCAAGCAGCGTTCCGAATATTCCTCAGTCACGTTACACTAGTGTGAATTTACATCAGCCGGTGAATGTACAACAACCGGTGCAACCAGACACAATCAAGATAGCTTGCCCCTCGTGTAAAAGAAAGTACGATTACAACTTTGCAAATCGACTCATATTTTGTCAAAGATGCCTAGGAATCTTTGATGCCTCTGATTTGGGTCCAGAATCTCTTGGGAATCCAGTAGCTCCAAGTCAAGGTTCTTCAAAAGAAGCTTCACAAAGTAATGGAGGGACTGGATACCCATCTTCAACAACTAATGGAAATGCAACGTCAAAGGCGGCAAGTGCTACTGAAGGTGGTGGAGCCTCTAGGACTGTAAAGAGAGATATCGAGAATGGAGTTGGAATTGAGAAGCAAGGGGTTGAAAGGTCTGAGTCTGGTCCACTGAAGTCAAAGAATTCAAGAAAATCAAGAAACAGAAAAAGAGGAGAGAATACTTTTGATTCTGGTGAAAGTTCCAAGACTGGAAAAGAAAAGGTTGGTATTCCTTCTGAAGTCTGTGAGCGTCACACCAGGAGATCTTCAAGGAGTATGCATAATCATTCCTTTTATGAAACTGTAAATGATGATGATGATGATGATGATGATGATGATGAAAATGATGATGATATTGGCAATGTCAAGGTGAATGATGATGTCAATGTGAGTAGGGAAAGCCCATTTCCCACTGCTACTAGAAAGAAGAGGGCTAATGCATCTGTGGATGGTGGAGTGTATAGGCAAACTGTCTTGGCCCTTTGTGCTATGGATGAGTATAGGAAATTAGGAAAACAAAGAGTGAGGCGCAAAAGAAGCAACACTGATGAAATTGAGTTACAGGTAAATGAATTATCCATGGCAGATAGCAATGGCAGCAACAAGTCCGTGGCAGATAACAGTGGCAGCAAGTCCATGACATCCAGGCGAAAGATCATCGAGGTTCCTGAACCTGAGTTCCATAGGTTTGTGCTCAGTAAAGATGTGTTACAGAAAACTTTTAAAGCCAATCAAACATGGGTTCTTTATGATCCAGCAGATGGCATGCCAAGACGCTATGCATGTATCAAGAAAGTCTTACACCCTGGATTTAGGGTGAAAATCACCTGGCTGGAGCCCGATCCCGATGACCAAGGTGAGGTTGATTGGTGTAGCAAAAAGTTGCCAGTTGCATGTGGTAAGTTTGGATTTGCGCTCACTGAAGAAGTTGCAGATCATACTGTGTTTTCTCATGAGTATATACACTCCACAAAAGGCAAGGATAAAAGCTCTTTTCGGATATATCCTAGGAAGGGAGAAACCTGGGCCCTCTACCAGGATTGGGATTTGGGGTGGAGTTCTGAGCCTGAAAAGCATTCACAATACAAGTTTTATTTTGTAGAAGTCCTGTCAGATTTTTCTGAAAAGTTTGGCGCTGGAGTTGCTTATTTAGGCAAAGTAAAAGGATTTGTTAGTTTGTTTGAGCCAGCTGAGCAGCAGGGGGTTCTCAAGTTTCAAGTTCCACCTAATGAGTTGTATAGATTTTCTCATCAAATTCCCTGTTACAAGATGTGTGGTAGTGAAGGATATGGTGTTCCTGCTGGTTCTTTTGAGTTTGATCCTGCTTCTTTGCCTGCCAGTCTTTTCGATCGAAGAAGCACTGTCACCATAGCCAAACCTTCTGGCTTCTTACCTTCTGGTAGAGCTCGCCCTGCTAAGAAAGAGGTGAAGAAAGATTCTGAAAGGGGAGGAGTCAAGAACGGGAACCTCAACGATGAACCAATTCTGATAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

753

Amino Acids

83.95

Weight (kDa)

8.5

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 128 1.2e-17 DnaJ domain
DUF3444 PF11926 473 - 683 2.9e-71 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 407
AccB1I GGYRCC 1 cut(s) 407
AccB7I CCANNNNNTGG 1 cut(s) 1526
AciI CCGC 2 cut(s) 311, 788
AclWI GGATC 5 cut(s) 49, 196, 1511, 1601, 2078
AcsI RAATTY 3 cut(s) 514, 907, 2019
AcuI CTGAAG 5 cut(s) 200, 822, 917, 1023, 1707
AfaI GTAC 3 cut(s) 409, 537, 596
AfeI AGCGCT 1 cut(s) 397
AfiI CCNNNNNNNGG 7 cut(s) 64, 704, 1526, 1624, 1778, 1801, 1807
AgeI ACCGGT 1 cut(s) 544
AgsI TTSAA 6 cut(s) 714, 759, 875, 912, 1035, 1982
AhlI ACTAGT 1 cut(s) 506
AjnI CCWGG 6 cut(s) 1022, 1409, 1570, 1592, 1787, 1800
AjuI GAANNNNNNNTTGG 2 cut(s) 691, 723
AleI CACNNNNGTG 1 cut(s) 509
AloI GAACNNNNNNTCC 4 cut(s) 1428, 1460, 2043, 2075
AluBI AGCT 8 cut(s) 121, 328, 572, 695, 722, 1755, 1955, 2171
AluI AGCT 8 cut(s) 121, 328, 572, 695, 722, 1755, 1955, 2171
Alw21I GWGCWC 2 cut(s) 1461, 2173
AlwI GGATC 5 cut(s) 49, 196, 1511, 1601, 2078
Aor51HI AGCGCT 1 cut(s) 397
AoxI GGCC 2 cut(s) 1242, 1791
ApaI GGGCCC 1 cut(s) 1795
ApeKI GCWGC 7 cut(s) 274, 317, 467, 474, 1362, 1392, 1960
ApoI RAATTY 3 cut(s) 514, 907, 2019
ArsI GACNNNNNNTTYG 2 cut(s) 609, 641
AsiGI ACCGGT 1 cut(s) 544
Asp700I GAANNNNTTC 2 cut(s) 370, 2247
Asp718I GGTACC 1 cut(s) 407
AspA2I CCTAGG 2 cut(s) 640, 1772
AspLEI GCGC 4 cut(s) 398, 1296, 1681, 1901
AspS9I GGNCC 6 cut(s) 449, 668, 890, 1243, 1791, 1792
AsuHPI GGTGA 8 cut(s) 541, 971, 1147, 1582, 1594, 1634, 2122, 2203
AvaII GGWCC 3 cut(s) 449, 668, 890
AvrII CCTAGG 2 cut(s) 640, 1772
BaeGI GKGCMC 1 cut(s) 1795
BanI GGYRCC 1 cut(s) 407
BanII GRGCYC 3 cut(s) 1605, 1795, 2173
BauI CACGAG 1 cut(s) 580
Bbv12I GWGCWC 2 cut(s) 1461, 2173
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 7 cut(s) 261, 304, 454, 486, 1374, 1404, 1972
BccI CCATC 4 cut(s) 82, 760, 1208, 1520
BciT130I CCWGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
BciVI GTATCC 1 cut(s) 740
BcuI ACTAGT 1 cut(s) 506
BfaI CTAG 6 cut(s) 507, 641, 819, 1185, 1773, 2260
BfoI RGCGCY 2 cut(s) 399, 1902
BfuI GTATCC 1 cut(s) 740
BglII AGATCT 1 cut(s) 1028
BisI GCNGC 8 cut(s) 275, 318, 468, 475, 789, 1363, 1393, 1961
BlnI CCTAGG 2 cut(s) 640, 1772
BlpI GCTNAGC 1 cut(s) 1956
BlsI GCNGC 8 cut(s) 276, 319, 469, 476, 790, 1364, 1394, 1962
Bme1390I CCNGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
Bme18I GGWCC 3 cut(s) 449, 668, 890
BmgT120I GGNCC 6 cut(s) 449, 668, 890, 1243, 1791, 1792
BmiI GGNNCC 8 cut(s) 409, 451, 669, 814, 1431, 1602, 1793, 2231
BmrFI CCNGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 3 cut(s) 626, 643, 1214
BmuI ACTGGG 1 cut(s) 315
BpmI CTGGAG 2 cut(s) 1619, 1923
Bpu10I CCTNAGC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 1956
BpuEI CTTGAG 1 cut(s) 1958
BsaBI GATNNNNATC 1 cut(s) 751
BsaJI CCNNGG 8 cut(s) 361, 640, 1344, 1374, 1570, 1618, 1772, 1788
BsaWI WCCGGW 1 cut(s) 544
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 704, 1526, 1624, 1778, 1801, 1807
Bse118I RCCGGY 2 cut(s) 526, 544
Bse1I ACTGG 9 cut(s) 206, 310, 409, 462, 689, 748, 979, 1652, 2103
Bse3DI GCAATG 3 cut(s) 39, 1132, 1363
Bse8I GATNNNNATC 1 cut(s) 751
BseBI CCWGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
BseDI CCNNGG 8 cut(s) 361, 640, 1344, 1374, 1570, 1618, 1772, 1788
BseGI GGATG 4 cut(s) 283, 1219, 1264, 1406
BseJI GATNNNNATC 1 cut(s) 751
BseLI CCNNNNNNNGG 7 cut(s) 64, 704, 1526, 1624, 1778, 1801, 1807
BseMI GCAATG 3 cut(s) 39, 1132, 1363
BseMII CTCAG 7 cut(s) 131, 506, 873, 1431, 1474, 1818, 1947
BseNI ACTGG 9 cut(s) 206, 310, 409, 462, 689, 748, 979, 1652, 2103
BseRI GAGGAG 2 cut(s) 951, 2229
BseSI GKGCMC 1 cut(s) 1795
BseXI GCAGC 7 cut(s) 261, 304, 454, 486, 1374, 1404, 1972
Bsh1285I CGRYCG 1 cut(s) 2116
BshFI GGCC 2 cut(s) 1244, 1793
BshNI GGYRCC 1 cut(s) 407
BshTI ACCGGT 1 cut(s) 544
BsiEI CGRYCG 1 cut(s) 2116
BsiHKAI GWGCWC 2 cut(s) 1461, 2173
BsiSI CCGG 2 cut(s) 527, 545
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 7 cut(s) 64, 704, 1526, 1624, 1778, 1801, 1807
BsmFI GGGAC 1 cut(s) 754
BsmI GAATGC 2 cut(s) 210, 1840
BsnI GGCC 2 cut(s) 1244, 1793
Bsp120I GGGCCC 1 cut(s) 1791
Bsp1286I GDGCHC 4 cut(s) 1461, 1605, 1795, 2173
Bsp1407I TGTACA 1 cut(s) 535
Bsp1720I GCTNAGC 1 cut(s) 1956
Bsp19I CCATGG 1 cut(s) 1344
BspACI CCGC 2 cut(s) 311, 788
BspANI GGCC 2 cut(s) 1244, 1793
BspCNI CTCAG 7 cut(s) 130, 505, 874, 1432, 1473, 1819, 1948
BspHI TCATGA 1 cut(s) 1717
BspLI GGNNCC 8 cut(s) 409, 451, 669, 814, 1431, 1602, 1793, 2231
BspPI GGATC 5 cut(s) 49, 196, 1511, 1601, 2078
BspT107I GGYRCC 1 cut(s) 407
BsrDI GCAATG 3 cut(s) 39, 1132, 1363
BsrFI RCCGGY 2 cut(s) 526, 544
BsrGI TGTACA 1 cut(s) 535
BsrI ACTGG 9 cut(s) 206, 310, 409, 462, 689, 748, 979, 1652, 2103
BssAI RCCGGY 2 cut(s) 526, 544
BssECI CCNNGG 8 cut(s) 361, 640, 1344, 1374, 1570, 1618, 1772, 1788
BssSI CACGAG 1 cut(s) 580
BssT1I CCWWGG 5 cut(s) 361, 640, 1344, 1618, 1772
Bst2BI CACGAG 1 cut(s) 580
Bst2UI CCWGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
Bst4CI ACNGT 8 cut(s) 148, 164, 826, 1069, 1237, 1388, 1708, 2128
Bst6I CTCTTC 1 cut(s) 1187
BstAUI TGTACA 1 cut(s) 535
BstC8I GCNNGC 6 cut(s) 472, 574, 1532, 1953, 2100, 2173
BstDEI CTNAG 8 cut(s) 117, 492, 882, 1440, 1460, 1827, 1956, 2181
BstDSI CCRYGG 2 cut(s) 1344, 1374
BstENI CCTNNNNNAGG 1 cut(s) 1799
BstF5I GGATG 4 cut(s) 283, 1219, 1264, 1406
BstH2I RGCGCY 2 cut(s) 399, 1902
BstHHI GCGC 4 cut(s) 398, 1296, 1681, 1901
BstMCI CGRYCG 1 cut(s) 2116
BstNI CCWGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
BstNSI RCATGY 3 cut(s) 1534, 1551, 1662
BstSCI CCNGG 6 cut(s) 1022, 1409, 1570, 1592, 1787, 1800
BstSLI GKGCMC 1 cut(s) 1795
BstV1I GCAGC 7 cut(s) 261, 304, 454, 486, 1374, 1404, 1972
BstX2I RGATCY 2 cut(s) 54, 1028
BstYI RGATCY 2 cut(s) 54, 1028
BsuI GTATCC 1 cut(s) 740
BsuRI GGCC 2 cut(s) 1244, 1793
BtgI CCRYGG 2 cut(s) 1344, 1374
BtsCI GGATG 4 cut(s) 283, 1219, 1264, 1406
BtsI GCAGTG 1 cut(s) 1176
BtsIMutI CAGTG 8 cut(s) 160, 469, 893, 1176, 1308, 1393, 1683, 2124
Cac8I GCNNGC 6 cut(s) 472, 574, 1532, 1953, 2100, 2173
CciI TCATGA 1 cut(s) 1717
CfoI GCGC 4 cut(s) 398, 1296, 1681, 1901
Cfr10I RCCGGY 2 cut(s) 526, 544
Cfr13I GGNCC 6 cut(s) 449, 668, 890, 1243, 1791, 1792
CseI GACGC 2 cut(s) 1004, 1548
Csp6I GTAC 3 cut(s) 408, 536, 595
CspAI ACCGGT 1 cut(s) 544
CspCI CAANNNNNGTGG 2 cut(s) 2020, 2055
CviAII CATG 7 cut(s) 1345, 1402, 1503, 1531, 1548, 1659, 1718
CviQI GTAC 3 cut(s) 408, 536, 595
DdeI CTNAG 8 cut(s) 117, 492, 882, 1440, 1460, 1827, 1956, 2181
DraI TTTAAA 2 cut(s) 73, 1489
Eam1104I CTCTTC 1 cut(s) 1187
EarI CTCTTC 1 cut(s) 1187
EciI GGCGGA 1 cut(s) 326
Ecl136II GAGCTC 1 cut(s) 2171
Eco130I CCWWGG 5 cut(s) 361, 640, 1344, 1618, 1772
Eco24I GRGCYC 3 cut(s) 1605, 1795, 2173
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 3 cut(s) 449, 668, 890
Eco47III AGCGCT 1 cut(s) 397
Eco53kI GAGCTC 1 cut(s) 2171
Eco57I CTGAAG 5 cut(s) 200, 822, 917, 1023, 1707
EcoICRI GAGCTC 1 cut(s) 2171
EcoNI CCTNNNNNAGG 1 cut(s) 1799
EcoO109I RGGNCCY 1 cut(s) 1792
EcoRI GAATTC 1 cut(s) 907
EcoRII CCWGG 6 cut(s) 1022, 1409, 1570, 1592, 1787, 1800
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 5 cut(s) 361, 640, 1344, 1618, 1772
EcoT22I ATGCAT 3 cut(s) 1047, 1207, 1549
EcoT38I GRGCYC 3 cut(s) 1605, 1795, 2173
ErhI CCWWGG 5 cut(s) 361, 640, 1344, 1618, 1772
FaeI CATG 7 cut(s) 1348, 1405, 1506, 1534, 1551, 1662, 1721
FalI AAGNNNNNCTT 2 cut(s) 1547, 1579
FaqI GGGAC 1 cut(s) 754
FatI CATG 7 cut(s) 1344, 1401, 1502, 1530, 1547, 1658, 1717
Fnu4HI GCNGC 8 cut(s) 275, 318, 468, 475, 789, 1363, 1393, 1961
FokI GGATG 4 cut(s) 270, 1226, 1271, 1393
FriOI GRGCYC 3 cut(s) 1605, 1795, 2173
Fsp4HI GCNGC 8 cut(s) 275, 318, 468, 475, 789, 1363, 1393, 1961
FspBI CTAG 6 cut(s) 507, 641, 819, 1185, 1773, 2260
GlaI GCGC 4 cut(s) 397, 1295, 1680, 1900
GluI GCNGC 8 cut(s) 275, 318, 468, 475, 789, 1363, 1393, 1961
GsuI CTGGAG 2 cut(s) 1619, 1923
HaeII RGCGCY 2 cut(s) 399, 1902
HaeIII GGCC 2 cut(s) 1244, 1793
HapII CCGG 2 cut(s) 527, 545
HgaI GACGC 2 cut(s) 1004, 1548
HhaI GCGC 4 cut(s) 398, 1296, 1681, 1901
Hin1II CATG 7 cut(s) 1348, 1405, 1506, 1534, 1551, 1662, 1721
Hin6I GCGC 4 cut(s) 396, 1294, 1679, 1899
HinP1I GCGC 4 cut(s) 396, 1294, 1679, 1899
HindIII AAGCTT 1 cut(s) 720
HpaII CCGG 2 cut(s) 527, 545
HphI GGTGA 8 cut(s) 541, 971, 1147, 1582, 1594, 1634, 2122, 2203
Hpy166II GTNNAC 3 cut(s) 160, 303, 893
Hpy8I GTNNAC 3 cut(s) 160, 303, 893
HpyAV CCTTC 7 cut(s) 172, 797, 1008, 1771, 2042, 2154, 2169
HpyCH4III ACNGT 8 cut(s) 148, 164, 826, 1069, 1237, 1388, 1708, 2128
HpyCH4IV ACGT 2 cut(s) 499, 779
HpyF3I CTNAG 8 cut(s) 117, 492, 882, 1440, 1460, 1827, 1956, 2181
HpySE526I ACGT 2 cut(s) 499, 779
Hsp92II CATG 7 cut(s) 1348, 1405, 1506, 1534, 1551, 1662, 1721
HspAI GCGC 4 cut(s) 396, 1294, 1679, 1899
KpnI GGTACC 1 cut(s) 411
LmnI GCTCC 4 cut(s) 77, 700, 812, 1600
Lsp1109I GCAGC 7 cut(s) 261, 304, 454, 486, 1374, 1404, 1972
LweI GCATC 3 cut(s) 626, 643, 1214
MaeI CTAG 6 cut(s) 507, 641, 819, 1185, 1773, 2260
MaeII ACGT 2 cut(s) 499, 779
MaeIII GTNAC 7 cut(s) 495, 500, 1016, 1323, 1473, 2027, 2128
MboII GAAGA 8 cut(s) 206, 702, 747, 1023, 1204, 1700, 2130, 2206
MflI RGATCY 2 cut(s) 54, 1028
MhlI GDGCHC 4 cut(s) 1461, 1605, 1795, 2173
MlyI GAGTC 4 cut(s) 102, 612, 893, 2226
MmeI TCCRAC 1 cut(s) 832
Mph1103I ATGCAT 3 cut(s) 1047, 1207, 1549
MroXI GAANNNNTTC 2 cut(s) 370, 2247
MseI TTAA 6 cut(s) 72, 140, 324, 401, 438, 1488
MslI CAYNNNNRTG 2 cut(s) 462, 509
MspA1I CMGCKG 1 cut(s) 1955
MspI CCGG 2 cut(s) 527, 545
MspR9I CCNGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
Mva1269I GAATGC 2 cut(s) 210, 1840
MvaI CCWGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
NcoI CCATGG 1 cut(s) 1344
NlaIII CATG 7 cut(s) 1348, 1405, 1506, 1534, 1551, 1662, 1721
NlaIV GGNNCC 8 cut(s) 409, 451, 669, 814, 1431, 1602, 1793, 2231
NmuCI GTSAC 3 cut(s) 495, 1016, 2128
NsiI ATGCAT 3 cut(s) 1047, 1207, 1549
NspI RCATGY 3 cut(s) 1534, 1551, 1662
OliI CACNNNNGTG 1 cut(s) 509
PaeI GCATGC 1 cut(s) 1534
PagI TCATGA 1 cut(s) 1717
PctI GAATGC 2 cut(s) 210, 1840
PdmI GAANNNNTTC 2 cut(s) 370, 2247
PfeI GAWTC 6 cut(s) 220, 645, 674, 685, 953, 2201
PflMI CCANNNNNTGG 1 cut(s) 1526
PinAI ACCGGT 1 cut(s) 544
PkrI GCNGC 8 cut(s) 276, 319, 469, 476, 790, 1364, 1394, 1962
Ple19I CGATCG 1 cut(s) 2116
PleI GAGTC 4 cut(s) 102, 612, 892, 2225
PpsI GAGTC 4 cut(s) 102, 612, 892, 2225
Psp124BI GAGCTC 1 cut(s) 2173
Psp6I CCWGG 6 cut(s) 1022, 1409, 1570, 1592, 1787, 1800
PspGI CCWGG 6 cut(s) 1022, 1409, 1570, 1592, 1787, 1800
PspN4I GGNNCC 8 cut(s) 409, 451, 669, 814, 1431, 1602, 1793, 2231
PspOMI GGGCCC 1 cut(s) 1791
PspPI GGNCC 6 cut(s) 449, 668, 890, 1243, 1791, 1792
PsrI GAACNNNNNNTAC 2 cut(s) 141, 173
PsuI RGATCY 2 cut(s) 54, 1028
PvuI CGATCG 1 cut(s) 2116
PvuII CAGCTG 1 cut(s) 1955
RsaI GTAC 3 cut(s) 409, 537, 596
RsaNI GTAC 3 cut(s) 408, 536, 595
RseI CAYNNNNRTG 2 cut(s) 462, 509
SacI GAGCTC 1 cut(s) 2173
SaqAI TTAA 6 cut(s) 72, 140, 324, 401, 438, 1488
SatI GCNGC 8 cut(s) 275, 318, 468, 475, 789, 1363, 1393, 1961
Sau96I GGNCC 6 cut(s) 449, 668, 890, 1243, 1791, 1792
SchI GAGTC 4 cut(s) 102, 612, 893, 2226
ScrFI CCNGG 6 cut(s) 1024, 1411, 1572, 1594, 1789, 1802
SduI GDGCHC 4 cut(s) 1461, 1605, 1795, 2173
SfaNI GCATC 3 cut(s) 626, 643, 1214
SinI GGWCC 3 cut(s) 449, 668, 890
SmiMI CAYNNNNRTG 2 cut(s) 462, 509
SmlI CTYRAG 1 cut(s) 1973
SmoI CTYRAG 1 cut(s) 1973
SpeI ACTAGT 1 cut(s) 506
SphI GCATGC 1 cut(s) 1534
SsiI CCGC 2 cut(s) 311, 788
SspI AATATT 1 cut(s) 487
SspMI CTAG 6 cut(s) 507, 641, 819, 1185, 1773, 2260
SstI GAGCTC 1 cut(s) 2173
StyD4I CCNGG 6 cut(s) 1022, 1409, 1570, 1592, 1787, 1800
StyI CCWWGG 5 cut(s) 361, 640, 1344, 1618, 1772
TaaI ACNGT 8 cut(s) 148, 164, 826, 1069, 1237, 1388, 1708, 2128
TaiI ACGT 2 cut(s) 502, 782
TaqI TCGA 5 cut(s) 616, 840, 1425, 2112, 2116
TatI WGTACW 1 cut(s) 535
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 6 cut(s) 220, 645, 674, 685, 953, 2201
Tru1I TTAA 6 cut(s) 72, 140, 324, 401, 438, 1488
Tru9I TTAA 6 cut(s) 72, 140, 324, 401, 438, 1488
TscAI CASTG 8 cut(s) 167, 469, 900, 1183, 1315, 1393, 1690, 2131
TseFI GTSAC 3 cut(s) 495, 1016, 2128
TseI GCWGC 7 cut(s) 274, 317, 467, 474, 1362, 1392, 1960
Tsp45I GTSAC 3 cut(s) 495, 1016, 2128
TspDTI ATGAA 7 cut(s) 252, 272, 1077, 1119, 1329, 1350, 2256
TspGWI ACGGA 1 cut(s) 1363
TspRI CASTG 8 cut(s) 167, 469, 900, 1183, 1315, 1393, 1690, 2131
Van91I CCANNNNNTGG 1 cut(s) 1526
VpaK11BI GGWCC 3 cut(s) 449, 668, 890
XagI CCTNNNNNAGG 1 cut(s) 1799
XapI RAATTY 3 cut(s) 514, 907, 2019
XceI RCATGY 3 cut(s) 1534, 1551, 1662
XmaJI CCTAGG 2 cut(s) 640, 1772
XmnI GAANNNNTTC 2 cut(s) 370, 2247
XspI CTAG 6 cut(s) 507, 641, 819, 1185, 1773, 2260
Zsp2I ATGCAT 3 cut(s) 1047, 1207, 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.