FvH4_6g04761

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000587
Physical Location & Seq
Forward (+)
2625519 .. 2630810
5292 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04761.t1

Sequence Viewer

Length: 3357 bp
ATGGAGTGCAACAAAGAGGAGGCAGTCAGAGCTATGCAACTTTCAGAAACAAAGATGCAGAATAAAGATTATACAGGAGCAATGAAGATGGCACAAAAGGCTCAGAGACTCTTCCCTGATCTTGAGAACATTTCTCAGTTGCTTACGGTTTGTGAAATTCACTGTACAGCAGGAAACAAGCTAGGTGGATCTGAAATGGATTGGTACCGAATTCTTCAGATACAGCAATTTGATAGTGATGACACCATCAGCAAACAGTATAAAAAACTGGCACTGTTGCTTCATCCTGATAAGAATAAGTTTGCTGGTTCGGAGACTGCTTTCAAGTGGATTGTGGAAGCAAAAAGTGTGCTGTTGGACAAACAAAAACGTTCTATATATGACATCAAGTATAGAACTCTAGCAAGACCTGGTGCACCAAAAACAACAGCTCATCCTGCTGCAATTAATCCTCAGAAAAATCCTCAGTCACAGGGGGAACGAAACACATTCTGGACACAGTGTTACTTTTGTCTCTCAAAGTATCAATACTATAGAGACTTTCTCGGTCGGTTGCTCCGTTGTCAAAGATGTCGGAATGCATTTGAAGCTCATGAATTACGGGAAGGTGTTCATTCAGAATCTTTTAGGAATGACTTCCCAAATCACAAAGAGCCCCCAAGGCAGGGTCCTTCAAACTTTGCCTCACAAAGTAATGGAGGAACTGAAAAAGGAAATTTCACAAGATTTCAGAATGGAAATGCAGCCTTTAAACCTGCATCAAAGGTATCTGCTGACTTGAGTGGGGCCTGTGAAGCTAAAAAGAAAGATATTAGGCATGAGGTTGAACTGGGGAAGCAAGGTGTTGAAACATCCAAGTCCAGTCCCCTAAAGTCCAAAGATACAGGAACTTCAAGTAATATTAACAAAGAAAGAGAGAAGACAACTTCTGAACTTGGAGAAGGTTTGAAGATGGGAAATAGAACTACAACTGATACTGAACATGCTGTTAAAGAGAAGGTTGAACTCAGTGCAGGTCATCACCCCAGGAGATCTTCAAGGAACAAGCATAATCTTTCATCCCTTCTAAATGACATGGATAATGATGATTTTGTGAGTCCCCCACTCAAAAGGTTGAGGAAGGACCAGCTGTTCAGTGCTGCTGAGAAGAAGGGGAATGATGCTGCTGTTGATGGAAATAAGAAAGATACAAGAGAAGAAGTAAGTTTTCCTGTAGAAGAAAATTTGCCAAACAAGAAGACTAAAACAGAGAAATTTGAGTTGAAGGTAAAGGGAGCCGCCATGTCGGTTAACGATCAAAGCAACTCCAAAGCTGATGTCAGTCCTGTAGCTAATGTAGATGTCACATCCACTCAGGGAGCCACCATGTCCGTTAACGATCAAAGCAACTCCAAAGCTGATGTCAATCCTGTAGCTAATGTAGATGTCACATCCACTCAGGGAGCCGCCATGTCGGTTAACGATCAAAGCAACTCCAAAGCTGATGTCAGTCCTGTAGCTAATGTAGATGTCACATCCACTCAGGGAGCCTTTGAACTGCCTGATCCTCAATTCCATAAGTTTCTGCTTGATGCAGATAGTTTACCAAGTCTTGTTAAAGCTAATCAAATATGGGCTCTTTATGATGATGACAGAGATAGTATGCCCAGATGGTATGCTTTTGTCAAGAAGGTCGTCACGCCTGGATTTAAGCTGAAAATCATCTGGCTGGAGCCCAATCCAAATGACCGAGGTGAGATTGATTGGTGTAAGGACTTGCCAGTTGCTTGTGGCAAGTTCAAATTAGGGGACACTGACGAAATTAAAGATCATCTCTCGTTTTCTCATCAGGTGGACTACAGAAAAGGGAGAGGTAGAAATTCTATTTTGGTATATCCTAGGAAGGGTGAAACTTGGGCCATCTACCAGAATTGGGATATTGGATGGAGTTCTGAGCCGGAAAAGCACATGCCCTACAAATATGACTTTGTTGAAGTGTTGACAGATTTTGTAGAAGCTGATGGAATCGGAGTTCGTTATTTGGGCAAAGTGAGAGGATTTGTCAGTTTATTTCAGAAAACCGAGCAGCACGGGGTTGTCATGTTTCAAGTACCACCTCATGAGCTATACAGATTTTCTCATCAGATTCCCTCTTTCAAAATGACTGGTTGTGAAGGACATGGTGTTCCCCCAGGATCCTTTGAACTTGATCCTGCTTCTTTGCCCACCATTATTTTTAATTCAAGTGATCTGGGTGATCTAGAAATGGATGACAGAAGCAAGAAAACTGAAATTGTTGGTCCGTCTTGTGAAAAGGTTGGTTCTGTAACTGCATGCACAGGTATGAAGCAGGAGAAGAATCTTTCCGAAAGGGAGACGTTGATGTCTAGATCTCCAAGAAAGTCAGATACTGAATTCGCGAATTGTATGAAAGGGAAGTCCATGACAAACCTAAACCGTGGAAACCTCACACAACCCAAGGATAGTGCTATACCATGTCAAGCAGATAAGAGAAACAACACACAGAAGAAGCATCAGAAGAACGATGCAGATAGAGCACCTTTTAGTCTTCGAAGATCACGAAGAGAATTAAGCAAGAACAGTACCACATCAAATGGAGCTATGAAGTGTCCAGATTCTGCCAAGGATGAAAATCATATCAGCTTTCCTCGCGCTAACTCTACCTCTAGCCAGTACAATAACAGGATGCAATCAACTCTGAAGGATCACGATTCTCACAGTCACATGAAAAACCCTTTAGCCCCTCCGCCATCTTCATCCCCTGCCTGCAGATTGTCCCAGGCTGAATTCTTCGATTTCAAAGGGCTGAAAACTCAGGACAGATTTGGTATTGGTCAAATTTGGGCTCTGTATAGTGATGTGAACGGGATGCCTAATACTTATGCTCAAGTTAGGGGGATCCAGGTTAGGCCAAAATTTCGAGTGCATGTGGCAGTGCTGGAACCTTGCTCAGAATTGAAACATTTGTCTGGACCAGTTTCCTGTGGGACATTTAAATTGAAAGATTGTCCAACTGAAGTCATCTCCCTCTCCTCATTTTCTCACTGCTTAAATACTGGAAATGTTTTGAGTAAGGAGGTGTTTGAAATAAAGCCAAAGAAAGGTGAGGTTTGGGCTCTATACAAGAACCACAATCCTGAGCCGGCTGGTCCTGACCTGGCAAAGGGTGAATGTGAATTAGTGGAAGTGTTGGAAGATGATGGCAGAAGCACAAAGGTTGGGGTTCTGGCTGAGTTTCATAGATTTTCTCACCAGATTCCTGCTTTCCAGCATCGTGGGGAGAGTGACAGTCGAGTTGCAGGTTGTTGGGAGCTGGATCCATTATCAATACCTGGTACTGTAATTTCCTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

1119

Amino Acids

125.03

Weight (kDa)

8.55

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 128 6.9e-18 DnaJ domain
DUF3444 PF11926 510 - 717 5.2e-72 Domain of unknown function (DUF3444)
DUF3444 PF11926 924 - 1076 1.8e-35 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2362
Acc36I ACCTGC 3 cut(s) 763, 1004, 3290
Acc65I GGTACC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 204
AccII CGCG 2 cut(s) 2399, 2652
AciI CCGC 3 cut(s) 1278, 1446, 2747
AclI AACGTT 1 cut(s) 370
AcuI CTGAAG 3 cut(s) 200, 2720, 3036
AfaI GTAC 6 cut(s) 166, 206, 2091, 2584, 2675, 3337
AfiI CCNNNNNNNGG 6 cut(s) 664, 665, 1883, 1912, 3101, 3163
AjnI CCWGG 8 cut(s) 409, 1025, 1681, 2170, 2778, 2901, 3156, 3331
AloI GAACNNNNNNTCC 6 cut(s) 1115, 1147, 1995, 2027, 2148, 2180
Alw21I GWGCWC 2 cut(s) 418, 2539
Alw26I GTCTC 5 cut(s) 100, 308, 518, 531, 2348
Alw44I GTGCAC 1 cut(s) 414
AlwNI CAGNNNCTG 2 cut(s) 2390, 2618
AoxI GGCC 3 cut(s) 786, 1896, 2909
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 5 cut(s) 440, 743, 1139, 1163, 2065
ArsI GACNNNNNNTTYG 6 cut(s) 1302, 1334, 1386, 1418, 1470, 1502
AseI ATTAAT 1 cut(s) 447
Asp700I GAANNNNTTC 2 cut(s) 609, 635
Asp718I GGTACC 1 cut(s) 204
AspA2I CCTAGG 1 cut(s) 1877
AspLEI GCGC 1 cut(s) 2654
AspS9I GGNCC 7 cut(s) 668, 786, 1123, 1896, 2279, 2972, 3149
AsuHPI GGTGA 7 cut(s) 1013, 1745, 1898, 2246, 3116, 3179, 3242
AsuII TTCGAA 1 cut(s) 2551
AvaII GGWCC 5 cut(s) 668, 1123, 2279, 2972, 3149
AvrII CCTAGG 1 cut(s) 1877
BaeGI GKGCMC 1 cut(s) 418
BamHI GGATCC 3 cut(s) 2174, 2898, 3316
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 5 cut(s) 657, 1618, 1716, 2848, 3118
BbsI GAAGAC 3 cut(s) 926, 1244, 2540
Bbv12I GWGCWC 2 cut(s) 418, 2539
BbvI GCAGC 5 cut(s) 427, 755, 1126, 1150, 2077
BciT130I CCWGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
BcoDI GTCTC 5 cut(s) 100, 308, 518, 531, 2348
BfaI CTAG 6 cut(s) 182, 401, 1878, 2240, 2367, 2667
BfmI CTRYAG 7 cut(s) 532, 1212, 1326, 1410, 1494, 1837, 2767
BfuAI ACCTGC 3 cut(s) 763, 1004, 3290
BglI GCCNNNNNGGC 1 cut(s) 661
BglII AGATCT 2 cut(s) 1031, 2369
BisI GCNGC 7 cut(s) 441, 744, 1140, 1164, 1278, 1446, 2066
BlnI CCTAGG 1 cut(s) 1877
BlsI GCNGC 7 cut(s) 442, 745, 1141, 1165, 1279, 1447, 2067
Bme1390I CCNGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
Bme18I GGWCC 5 cut(s) 668, 1123, 2279, 2972, 3149
BmgT120I GGNCC 7 cut(s) 668, 786, 1123, 1896, 2279, 2972, 3149
BmrFI CCNGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
BmrI ACTGGG 1 cut(s) 839
BmsI GCATC 9 cut(s) 45, 767, 1150, 1561, 2515, 2521, 2676, 2859, 3280
BmuI ACTGGG 1 cut(s) 839
BpiI GAAGAC 3 cut(s) 926, 1244, 2540
BplI GAGNNNNNCTC 2 cut(s) 528, 560
BpmI CTGGAG 1 cut(s) 1730
Bpu10I CCTNAGC 1 cut(s) 3138
Bpu14I TTCGAA 1 cut(s) 2551
BpuEI CTTGAG 3 cut(s) 143, 799, 2871
BsaBI GATNNNNATC 2 cut(s) 1404, 2631
BsaJI CCNNGG 9 cut(s) 659, 1025, 1729, 1877, 2170, 2437, 2457, 2622, 2778
Bsc4I CCNNNNNNNGG 6 cut(s) 664, 665, 1883, 1912, 3101, 3163
Bse118I RCCGGY 1 cut(s) 3142
Bse1I ACTGG 8 cut(s) 273, 834, 861, 1760, 2149, 2671, 2975, 3061
Bse3DI GCAATG 1 cut(s) 87
Bse8I GATNNNNATC 2 cut(s) 1404, 2631
BseBI CCWGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
BseDI CCNNGG 9 cut(s) 659, 1025, 1729, 1877, 2170, 2437, 2457, 2622, 2778
BseJI GATNNNNATC 2 cut(s) 1404, 2631
BseLI CCNNNNNNNGG 6 cut(s) 664, 665, 1883, 1912, 3101, 3163
BseMI GCAATG 1 cut(s) 87
BseNI ACTGG 8 cut(s) 273, 834, 861, 1760, 2149, 2671, 2975, 3061
BseRI GAGGAG 2 cut(s) 32, 3022
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 5 cut(s) 427, 755, 1126, 1150, 2077
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 2 cut(s) 2399, 2652
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 3 cut(s) 788, 1898, 2911
BshNI GGYRCC 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 2 cut(s) 418, 2539
BsiSI CCGG 2 cut(s) 1937, 3143
BslFI GGGAC 5 cut(s) 849, 1083, 1802, 2761, 3001
BslI CCNNNNNNNGG 6 cut(s) 664, 665, 1883, 1912, 3101, 3163
BsmAI GTCTC 5 cut(s) 100, 308, 518, 531, 2348
BsmBI CGTCTC 1 cut(s) 2348
BsmFI GGGAC 5 cut(s) 849, 1083, 1802, 2761, 3001
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 3 cut(s) 788, 1898, 2911
Bsp119I TTCGAA 1 cut(s) 2551
Bsp1286I GDGCHC 7 cut(s) 418, 657, 1618, 1716, 2539, 2848, 3118
Bsp1407I TGTACA 1 cut(s) 164
Bsp68I TCGCGA 1 cut(s) 2399
BspACI CCGC 3 cut(s) 1278, 1446, 2747
BspANI GGCC 3 cut(s) 788, 1898, 2911
BspFNI CGCG 2 cut(s) 2399, 2652
BspHI TCATGA 2 cut(s) 592, 2098
BspMAI CTGCAG 1 cut(s) 2771
BspMI ACCTGC 3 cut(s) 763, 1004, 3290
BspT104I TTCGAA 1 cut(s) 2551
BspT107I GGYRCC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 3142
BsrGI TGTACA 1 cut(s) 164
BsrI ACTGG 8 cut(s) 273, 834, 861, 1760, 2149, 2671, 2975, 3061
BssAI RCCGGY 1 cut(s) 3142
BssECI CCNNGG 9 cut(s) 659, 1025, 1729, 1877, 2170, 2437, 2457, 2622, 2778
BssT1I CCWWGG 4 cut(s) 659, 1877, 2457, 2622
Bst2UI CCWGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
Bst6I CTCTTC 2 cut(s) 116, 2557
BstAUI TGTACA 1 cut(s) 164
BstBI TTCGAA 1 cut(s) 2551
BstC8I GCNNGC 3 cut(s) 2314, 2767, 3144
BstDSI CCRYGG 1 cut(s) 2437
BstENI CCTNNNNNAGG 1 cut(s) 3161
BstFNI CGCG 2 cut(s) 2399, 2652
BstHHI GCGC 1 cut(s) 2654
BstMAI GTCTC 5 cut(s) 100, 308, 518, 531, 2348
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 9 cut(s) 29, 98, 437, 587, 661, 794, 1942, 2534, 2649
BstNI CCWGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
BstNSI RCATGY 4 cut(s) 986, 1951, 2316, 2930
BstSCI CCNGG 8 cut(s) 409, 1025, 1681, 2170, 2778, 2901, 3156, 3331
BstSFI CTRYAG 7 cut(s) 532, 1212, 1326, 1410, 1494, 1837, 2767
BstSLI GKGCMC 1 cut(s) 418
BstUI CGCG 2 cut(s) 2399, 2652
BstV1I GCAGC 5 cut(s) 427, 755, 1126, 1150, 2077
BstV2I GAAGAC 3 cut(s) 926, 1244, 2540
BstX2I RGATCY 6 cut(s) 188, 1031, 2174, 2369, 2898, 3316
BstXI CCANNNNNNTGG 1 cut(s) 3275
BstYI RGATCY 6 cut(s) 188, 1031, 2174, 2369, 2898, 3316
BsuRI GGCC 3 cut(s) 788, 1898, 2911
BtgI CCRYGG 1 cut(s) 2437
BtsI GCAGTG 2 cut(s) 2940, 3043
BtsIMutI CAGTG 8 cut(s) 160, 272, 506, 1015, 1141, 1791, 2940, 3043
BtuMI TCGCGA 1 cut(s) 2399
BveI ACCTGC 3 cut(s) 763, 1004, 3290
Cac8I GCNNGC 3 cut(s) 2314, 2767, 3144
CaiI CAGNNNCTG 2 cut(s) 2390, 2618
CciI TCATGA 2 cut(s) 592, 2098
CfoI GCGC 1 cut(s) 2654
Cfr10I RCCGGY 1 cut(s) 3142
Cfr13I GGNCC 7 cut(s) 668, 786, 1123, 1896, 2279, 2972, 3149
CsiI ACCWGGT 2 cut(s) 409, 3331
Csp6I GTAC 6 cut(s) 165, 205, 2090, 2583, 2674, 3336
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviQI GTAC 6 cut(s) 165, 205, 2090, 2583, 2674, 3336
DraI TTTAAA 2 cut(s) 751, 2995
DrdI GACNNNNNNGTC 1 cut(s) 2362
DseDI GACNNNNNNGTC 1 cut(s) 2362
Eam1104I CTCTTC 2 cut(s) 116, 2557
EarI CTCTTC 2 cut(s) 116, 2557
EciI GGCGGA 1 cut(s) 2736
Eco130I CCWWGG 4 cut(s) 659, 1877, 2457, 2622
Eco24I GRGCYC 5 cut(s) 657, 1618, 1716, 2848, 3118
Eco47I GGWCC 5 cut(s) 668, 1123, 2279, 2972, 3149
Eco57I CTGAAG 3 cut(s) 200, 2720, 3036
EcoNI CCTNNNNNAGG 1 cut(s) 3161
EcoO109I RGGNCCY 2 cut(s) 668, 786
EcoRI GAATTC 3 cut(s) 210, 2393, 2786
EcoRII CCWGG 8 cut(s) 409, 1025, 1681, 2170, 2778, 2901, 3156, 3331
EcoT14I CCWWGG 4 cut(s) 659, 1877, 2457, 2622
EcoT22I ATGCAT 1 cut(s) 583
EcoT38I GRGCYC 5 cut(s) 657, 1618, 1716, 2848, 3118
ErhI CCWWGG 4 cut(s) 659, 1877, 2457, 2622
Esp3I CGTCTC 1 cut(s) 2348
FaqI GGGAC 5 cut(s) 849, 1083, 1802, 2761, 3001
Fnu4HI GCNGC 7 cut(s) 441, 744, 1140, 1164, 1278, 1446, 2066
FriOI GRGCYC 5 cut(s) 657, 1618, 1716, 2848, 3118
Fsp4HI GCNGC 7 cut(s) 441, 744, 1140, 1164, 1278, 1446, 2066
FspBI CTAG 6 cut(s) 182, 401, 1878, 2240, 2367, 2667
GlaI GCGC 1 cut(s) 2653
GluI GCNGC 7 cut(s) 441, 744, 1140, 1164, 1278, 1446, 2066
GsuI CTGGAG 1 cut(s) 1730
HaeIII GGCC 3 cut(s) 788, 1898, 2911
HapII CCGG 2 cut(s) 1937, 3143
HhaI GCGC 1 cut(s) 2654
Hin6I GCGC 1 cut(s) 2652
HinP1I GCGC 1 cut(s) 2652
HincII GTYRAC 4 cut(s) 1291, 1375, 1459, 1980
HindII GTYRAC 4 cut(s) 1291, 1375, 1459, 1980
HinfI GANTC 9 cut(s) 108, 620, 1096, 2004, 2125, 2338, 2615, 2711, 3256
HpaI GTTAAC 3 cut(s) 1291, 1375, 1459
HpaII CCGG 2 cut(s) 1937, 3143
HphI GGTGA 7 cut(s) 1013, 1745, 1898, 2246, 3116, 3179, 3242
Hpy166II GTNNAC 8 cut(s) 416, 1291, 1375, 1459, 1583, 1834, 1980, 2863
Hpy8I GTNNAC 8 cut(s) 416, 1291, 1375, 1459, 1583, 1834, 1980, 2863
HpyCH4IV ACGT 2 cut(s) 370, 2357
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 98, 437, 587, 661, 794, 1942, 2534, 2649
HpySE526I ACGT 2 cut(s) 370, 2357
HspAI GCGC 1 cut(s) 2652
KpnI GGTACC 1 cut(s) 208
KroI GCCGGC 1 cut(s) 3142
KroNI GCCGGC 1 cut(s) 3144
KspAI GTTAAC 3 cut(s) 1291, 1375, 1459
LmnI GCTCC 9 cut(s) 77, 561, 1274, 1358, 1442, 1526, 1711, 2597, 3310
Lsp1109I GCAGC 5 cut(s) 427, 755, 1126, 1150, 2077
LweI GCATC 9 cut(s) 45, 767, 1150, 1561, 2515, 2521, 2676, 2859, 3280
MabI ACCWGGT 2 cut(s) 409, 3331
MaeI CTAG 6 cut(s) 182, 401, 1878, 2240, 2367, 2667
MaeII ACGT 2 cut(s) 370, 2357
MaeIII GTNAC 9 cut(s) 468, 503, 1342, 1426, 1510, 1675, 2305, 2720, 3284
MflI RGATCY 6 cut(s) 188, 1031, 2174, 2369, 2898, 3316
MhlI GDGCHC 7 cut(s) 418, 657, 1618, 1716, 2539, 2848, 3118
MlyI GAGTC 2 cut(s) 102, 1105
MmeI TCCRAC 4 cut(s) 336, 554, 3035, 3171
Mph1103I ATGCAT 1 cut(s) 583
MroNI GCCGGC 1 cut(s) 3142
MroXI GAANNNNTTC 2 cut(s) 609, 635
MslI CAYNNNNRTG 1 cut(s) 2321
MspA1I CMGCKG 1 cut(s) 1129
MspI CCGG 2 cut(s) 1937, 3143
MspR9I CCNGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
MvnI CGCG 2 cut(s) 2399, 2652
MwoI GCNNNNNNNGC 9 cut(s) 29, 98, 437, 587, 661, 794, 1942, 2534, 2649
NaeI GCCGGC 1 cut(s) 3144
NgoMIV GCCGGC 1 cut(s) 3142
NmuCI GTSAC 7 cut(s) 468, 1342, 1426, 1510, 1675, 2720, 3284
NruI TCGCGA 1 cut(s) 2399
NsiI ATGCAT 1 cut(s) 583
NspI RCATGY 4 cut(s) 986, 1951, 2316, 2930
NspV TTCGAA 1 cut(s) 2551
PaeI GCATGC 1 cut(s) 2316
PagI TCATGA 2 cut(s) 592, 2098
PcsI WCGNNNNNNNCGW 2 cut(s) 556, 2557
PctI GAATGC 1 cut(s) 583
PdiI GCCGGC 1 cut(s) 3144
PdmI GAANNNNTTC 2 cut(s) 609, 635
PfeI GAWTC 7 cut(s) 620, 2004, 2125, 2338, 2615, 2711, 3256
PkrI GCNGC 7 cut(s) 442, 745, 1141, 1165, 1279, 1447, 2067
PleI GAGTC 2 cut(s) 102, 1104
PpsI GAGTC 2 cut(s) 102, 1104
PpuMI RGGWCCY 1 cut(s) 668
PshBI ATTAAT 1 cut(s) 447
Psp1406I AACGTT 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 668
Psp6I CCWGG 8 cut(s) 409, 1025, 1681, 2170, 2778, 2901, 3156, 3331
PspGI CCWGG 8 cut(s) 409, 1025, 1681, 2170, 2778, 2901, 3156, 3331
PspPI GGNCC 7 cut(s) 668, 786, 1123, 1896, 2279, 2972, 3149
PspPPI RGGWCCY 1 cut(s) 668
PstI CTGCAG 1 cut(s) 2771
PstNI CAGNNNCTG 2 cut(s) 2390, 2618
PsuI RGATCY 6 cut(s) 188, 1031, 2174, 2369, 2898, 3316
PvuII CAGCTG 1 cut(s) 1129
RruI TCGCGA 1 cut(s) 2399
RsaI GTAC 6 cut(s) 166, 206, 2091, 2584, 2675, 3337
RsaNI GTAC 6 cut(s) 165, 205, 2090, 2583, 2674, 3336
RseI CAYNNNNRTG 1 cut(s) 2321
SatI GCNGC 7 cut(s) 441, 744, 1140, 1164, 1278, 1446, 2066
Sau96I GGNCC 7 cut(s) 668, 786, 1123, 1896, 2279, 2972, 3149
SchI GAGTC 2 cut(s) 102, 1105
ScrFI CCNGG 8 cut(s) 411, 1027, 1683, 2172, 2780, 2903, 3158, 3333
SduI GDGCHC 7 cut(s) 418, 657, 1618, 1716, 2539, 2848, 3118
SexAI ACCWGGT 2 cut(s) 409, 3331
SfaNI GCATC 9 cut(s) 45, 767, 1150, 1561, 2515, 2521, 2676, 2859, 3280
SfcI CTRYAG 7 cut(s) 532, 1212, 1326, 1410, 1494, 1837, 2767
SfuI TTCGAA 1 cut(s) 2551
SinI GGWCC 5 cut(s) 668, 1123, 2279, 2972, 3149
SmiI ATTTAAAT 1 cut(s) 2995
SmiMI CAYNNNNRTG 1 cut(s) 2321
SmlI CTYRAG 3 cut(s) 122, 778, 2886
SmoI CTYRAG 3 cut(s) 122, 778, 2886
SphI GCATGC 1 cut(s) 2316
SsiI CCGC 3 cut(s) 1278, 1446, 2747
SspI AATATT 1 cut(s) 901
SspMI CTAG 6 cut(s) 182, 401, 1878, 2240, 2367, 2667
StyD4I CCNGG 8 cut(s) 409, 1025, 1681, 2170, 2778, 2901, 3156, 3331
StyI CCWWGG 4 cut(s) 659, 1877, 2457, 2622
SwaI ATTTAAAT 1 cut(s) 2995
TaiI ACGT 2 cut(s) 373, 2360
TaqI TCGA 4 cut(s) 2551, 2793, 2920, 3292
TaqII GACCGA 2 cut(s) 536, 1743
TatI WGTACW 2 cut(s) 164, 2673
TauI GCSGC 2 cut(s) 1280, 1448
TfiI GAWTC 7 cut(s) 620, 2004, 2125, 2338, 2615, 2711, 3256
TscAI CASTG 8 cut(s) 167, 279, 506, 1015, 1141, 1798, 2940, 3050
TseFI GTSAC 7 cut(s) 468, 1342, 1426, 1510, 1675, 2720, 3284
TseI GCWGC 5 cut(s) 440, 743, 1139, 1163, 2065
Tsp45I GTSAC 7 cut(s) 468, 1342, 1426, 1510, 1675, 2720, 3284
TspGWI ACGGA 3 cut(s) 548, 1360, 2271
TspRI CASTG 8 cut(s) 167, 279, 506, 1015, 1141, 1798, 2940, 3050
VneI GTGCAC 1 cut(s) 414
VpaK11BI GGWCC 5 cut(s) 668, 1123, 2279, 2972, 3149
VspI ATTAAT 1 cut(s) 447
XagI CCTNNNNNAGG 1 cut(s) 3161
XbaI TCTAGA 2 cut(s) 2239, 2366
XceI RCATGY 4 cut(s) 986, 1951, 2316, 2930
XmaJI CCTAGG 1 cut(s) 1877
XmnI GAANNNNTTC 2 cut(s) 609, 635
XspI CTAG 6 cut(s) 182, 401, 1878, 2240, 2367, 2667
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.