RchiOBHm_Chr3g0453181

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000587
Physical Location & Seq
Forward (+)
3672684 .. 3675961
3278 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42029

Sequence Viewer

Length: 1470 bp
ATGGACTGCAACAAAGAGGAGGCAATCAGGGCAATGCAGCTGTCAGAAGAAAAGATGCAGCTTAATGATTTTGCAGGAGCAAGGAAGTTGGCTCAAACAGCTCAACGCCTCTGCTCTGAGCTTCAGAACATTTCTCAGCTACTAACAGCCTGTGAGGTTCAGCGATCTGATAATGATGAGAGCATAAAGAAACAATTCAGAAAACTAGCGCTATTACTTCATCCTGATAAGAATAAATTTGCTGGAGCTGAGGCTGCTTTTAAGCTGATCATGGATGCAAACAGGGTGCTGGCGGACCAAGGAACTCGTTCTAAGTATGACAAGAAGTATAGAAAAGCTTTAGCAAAAGCTGGTAGAATTGGAAGCTCTTCTGTACCAATACACAAAGGAAATGCAACCTTGAATCCGGAGTCAAAGGCAGGAATTGCTGCTGATGTTGGGACTTCTAACACTGTAAAGAAAGATATTGGGCATGCAGATGGAGTAGGCAAGCAAGGTGTTGATTCTTCCAGGTCTGGTCCAAGGTCCAAGGATCTAGGAACTTCGAGAAATATGAATAAAAAGAGAGGAAGAAGTTCAAGTTTTGAATCCACTGAAAATTCCAACAGAGGAAAGAGAGCTAGAGCTGATTCTGAGCTGAATGGAGGTGATCACCTTAGGAGATCTTCAAGGAGCAAGCAGAATCTTTCGTCCAGTGAAAAGCTAAATAATAATGGTGCTATGAATGGTGGGATATTAAAAAATGATAGGCCAACTGATTCTGGAGCCAATTCAGTTGTTGCATCCGTACCAAAGGTCTTTGAGGTTCCTGAACCTGAATTCAATAAGTTTGCGCCCGAGGGAAATATTTTTAATGCTAATCAAACATGGGCACTTTATGATCAAGTAGACGGCATGCCAAGGTTCTATGCTCGAGTTAAGAAAGTGCACACTCCCGGATTTAAGGTGAAAATCACCTGGCTCATTCCCAATCCAGATGACAAAGGTGAGATTGGTTGGGTTAAAGAAGACTTGCCAGTTGCTTGTGGTAAGTACACAATTGGTCTCACTGAAGACATTACAGATCATCTATCGTTTTCTCATCAGATGCATTGCACAAATGGCACCGTCAGAGACTCCTTTCTGGTATATCCTAGGAAGGGAGAAACTTGGGCCCTCTTCAAGAATTGGGACATTGGGTGGAGCTCTGACCCTGCAAAGCACAAGCCATTCGACTTCGAATTCGTGGAAGTCCTGTCAGATTTTTCACAAGTTTTTGGCACCAGAGTAGCTTATTTAGCCAAAGTCAAAGGCTTTGTGAGTCTGTTTAAGCGAAGCAAGCCGCATGGTGTCATTTCATTTCAAATACCACCTGATGAGGTGTACAGATTCTCGCATCGAATTCCCTCTTTCATGATGTCTGGTGCCGAAAGGCGTGGTGTTCCTAAAGGTTCCTTTGAGCTTGATCCAGCCTCTCTGCCTAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

489

Amino Acids

54.19

Weight (kDa)

9.48

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 52 - 107 9.3e-15 DnaJ domain
DUF3444 PF11926 265 - 470 1.8e-73 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 1103, 1259, 1403
AccI GTMKAC 1 cut(s) 888
AccIII TCCGGA 1 cut(s) 406
AciI CCGC 2 cut(s) 293, 1322
AclWI GGATC 2 cut(s) 540, 1439
AcsI RAATTY 5 cut(s) 236, 598, 818, 1220, 1380
AcuI CTGAAG 2 cut(s) 107, 1071
AfaI GTAC 4 cut(s) 375, 789, 1034, 1364
AfeI AGCGCT 1 cut(s) 210
AfiI CCNNNNNNNGG 1 cut(s) 1139
AgsI TTSAA 7 cut(s) 403, 579, 587, 669, 823, 1162, 1343
AjnI CCWGG 2 cut(s) 509, 956
Alw21I GWGCWC 2 cut(s) 930, 1187
Alw26I GTCTC 2 cut(s) 1049, 1107
Alw44I GTGCAC 1 cut(s) 926
AlwI GGATC 2 cut(s) 540, 1439
Ama87I CYCGRG 2 cut(s) 836, 912
Aor13HI TCCGGA 1 cut(s) 406
Aor51HI AGCGCT 1 cut(s) 210
AoxI GGCC 2 cut(s) 749, 1152
ApaI GGGCCC 1 cut(s) 1156
ApaLI GTGCAC 1 cut(s) 926
ApeKI GCWGC 4 cut(s) 37, 58, 254, 428
ApoI RAATTY 5 cut(s) 236, 598, 818, 1220, 1380
ArsI GACNNNNNNTTYG 2 cut(s) 1205, 1237
Asp700I GAANNNNTTC 4 cut(s) 194, 307, 367, 574
AspA2I CCTAGG 1 cut(s) 1133
AspLEI GCGC 2 cut(s) 211, 835
AspS9I GGNCC 5 cut(s) 295, 518, 525, 1152, 1153
AsuC2I CCSGG 1 cut(s) 936
AsuHPI GGTGA 5 cut(s) 644, 659, 946, 958, 998
AsuII TTCGAA 1 cut(s) 1218
AvaI CYCGRG 2 cut(s) 836, 912
AvaII GGWCC 3 cut(s) 295, 518, 525
AvrII CCTAGG 1 cut(s) 1133
AxyI CCTNAGG 1 cut(s) 656
BaeGI GKGCMC 3 cut(s) 874, 930, 1156
BanI GGYRCC 3 cut(s) 1103, 1259, 1403
BanII GRGCYC 2 cut(s) 1156, 1187
BbsI GAAGAC 2 cut(s) 1014, 1059
Bbv12I GWGCWC 2 cut(s) 930, 1187
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 4 cut(s) 49, 70, 241, 415
BccI CCATC 1 cut(s) 473
BceAI ACGGC 1 cut(s) 907
BciT130I CCWGG 2 cut(s) 511, 958
BclI TGATCA 3 cut(s) 267, 649, 880
BcnI CCSGG 1 cut(s) 936
BcoDI GTCTC 2 cut(s) 1049, 1107
BfaI CTAG 5 cut(s) 206, 536, 621, 1134, 1461
BfoI RGCGCY 1 cut(s) 212
BglII AGATCT 1 cut(s) 662
BisI GCNGC 5 cut(s) 38, 59, 255, 429, 1322
BlnI CCTAGG 1 cut(s) 1133
BlsI GCNGC 5 cut(s) 39, 60, 256, 430, 1323
Bme1390I CCNGG 3 cut(s) 511, 936, 958
Bme18I GGWCC 3 cut(s) 295, 518, 525
BmeT110I CYCGRG 2 cut(s) 836, 912
BmgT120I GGNCC 5 cut(s) 295, 518, 525, 1152, 1153
BmiI GGNNCC 7 cut(s) 766, 807, 1105, 1154, 1261, 1405, 1432
BmrFI CCNGG 3 cut(s) 511, 936, 958
BmsI GCATC 5 cut(s) 45, 265, 791, 1077, 1384
BpiI GAAGAC 2 cut(s) 1014, 1059
BpmI CTGGAG 2 cut(s) 264, 783
Bpu10I CCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 1 cut(s) 1218
BpuMI CCSGG 1 cut(s) 936
BsaI GGTCTC 1 cut(s) 1049
BsaJI CCNNGG 6 cut(s) 298, 521, 528, 837, 899, 1133
BsaWI WCCGGW 1 cut(s) 406
Bsc4I CCNNNNNNNGG 1 cut(s) 1139
Bse1I ACTGG 2 cut(s) 693, 1016
Bse21I CCTNAGG 1 cut(s) 656
Bse3DI GCAATG 2 cut(s) 39, 1090
BseAI TCCGGA 1 cut(s) 406
BseBI CCWGG 2 cut(s) 511, 958
BseDI CCNNGG 6 cut(s) 298, 521, 528, 837, 899, 1133
BseGI GGATG 3 cut(s) 220, 280, 782
BseLI CCNNNNNNNGG 1 cut(s) 1139
BseMI GCAATG 2 cut(s) 39, 1090
BseMII CTCAG 4 cut(s) 108, 149, 240, 624
BseNI ACTGG 2 cut(s) 693, 1016
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 3 cut(s) 874, 930, 1156
BseXI GCAGC 4 cut(s) 49, 70, 241, 415
BshFI GGCC 2 cut(s) 751, 1154
BshNI GGYRCC 3 cut(s) 1103, 1259, 1403
BsiHKAI GWGCWC 2 cut(s) 930, 1187
BsiHKCI CYCGRG 2 cut(s) 836, 912
BsiSI CCGG 2 cut(s) 407, 936
BslFI GGGAC 2 cut(s) 454, 1184
BslI CCNNNNNNNGG 1 cut(s) 1139
BsmAI GTCTC 2 cut(s) 1049, 1107
BsmFI GGGAC 2 cut(s) 454, 1184
BsnI GGCC 2 cut(s) 751, 1154
Bso31I GGTCTC 1 cut(s) 1049
BsoBI CYCGRG 2 cut(s) 836, 912
Bsp119I TTCGAA 1 cut(s) 1218
Bsp120I GGGCCC 1 cut(s) 1152
Bsp1286I GDGCHC 4 cut(s) 874, 930, 1156, 1187
Bsp13I TCCGGA 1 cut(s) 406
Bsp1407I TGTACA 1 cut(s) 1362
Bsp143I GATC 8 cut(s) 164, 267, 532, 649, 662, 880, 1063, 1444
BspACI CCGC 2 cut(s) 293, 1322
BspANI GGCC 2 cut(s) 751, 1154
BspCNI CTCAG 4 cut(s) 109, 148, 241, 625
BspEI TCCGGA 1 cut(s) 406
BspHI TCATGA 1 cut(s) 1392
BspLI GGNNCC 7 cut(s) 766, 807, 1105, 1154, 1261, 1405, 1432
BspPI GGATC 2 cut(s) 540, 1439
BspQI GCTCTTC 1 cut(s) 373
BspT104I TTCGAA 1 cut(s) 1218
BspT107I GGYRCC 3 cut(s) 1103, 1259, 1403
BspTNI GGTCTC 1 cut(s) 1049
BsrDI GCAATG 2 cut(s) 39, 1090
BsrGI TGTACA 1 cut(s) 1362
BsrI ACTGG 2 cut(s) 693, 1016
BssECI CCNNGG 6 cut(s) 298, 521, 528, 837, 899, 1133
BssMI GATC 8 cut(s) 164, 267, 532, 649, 662, 880, 1063, 1444
BssT1I CCWWGG 5 cut(s) 298, 521, 528, 899, 1133
Bst2UI CCWGG 2 cut(s) 511, 958
Bst4CI ACNGT 2 cut(s) 454, 1108
Bst6I CTCTTC 2 cut(s) 373, 1163
BstAPI GCANNNNNTGC 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 1362
BstBI TTCGAA 1 cut(s) 1218
BstC8I GCNNGC 6 cut(s) 291, 474, 491, 677, 896, 1319
BstDEI CTNAG 6 cut(s) 117, 135, 249, 312, 633, 656
BstF5I GGATG 3 cut(s) 220, 280, 782
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 2 cut(s) 211, 835
BstKTI GATC 8 cut(s) 167, 270, 535, 652, 665, 883, 1066, 1447
BstMAI GTCTC 2 cut(s) 1049, 1107
BstMBI GATC 8 cut(s) 164, 267, 532, 649, 662, 880, 1063, 1444
BstMWI GCNNNNNNNGC 7 cut(s) 29, 98, 254, 425, 1101, 1277, 1318
BstNI CCWGG 2 cut(s) 511, 958
BstNSI RCATGY 2 cut(s) 476, 898
BstSCI CCNGG 3 cut(s) 509, 934, 956
BstSLI GKGCMC 3 cut(s) 874, 930, 1156
BstV1I GCAGC 4 cut(s) 49, 70, 241, 415
BstV2I GAAGAC 2 cut(s) 1014, 1059
BstX2I RGATCY 2 cut(s) 532, 662
BstYI RGATCY 2 cut(s) 532, 662
Bsu36I CCTNAGG 1 cut(s) 656
BsuRI GGCC 2 cut(s) 751, 1154
BtsCI GGATG 3 cut(s) 220, 280, 782
BtsIMutI CAGTG 4 cut(s) 450, 591, 700, 1047
Cac8I GCNNGC 6 cut(s) 291, 474, 491, 677, 896, 1319
CciI TCATGA 1 cut(s) 1392
CfoI GCGC 2 cut(s) 211, 835
Cfr13I GGNCC 5 cut(s) 295, 518, 525, 1152, 1153
Csp6I GTAC 4 cut(s) 374, 788, 1033, 1363
CviAII CATG 6 cut(s) 271, 473, 867, 895, 1325, 1393
CviQI GTAC 4 cut(s) 374, 788, 1033, 1363
DdeI CTNAG 6 cut(s) 117, 135, 249, 312, 633, 656
DpnI GATC 8 cut(s) 166, 269, 534, 651, 664, 882, 1065, 1446
DpnII GATC 8 cut(s) 164, 267, 532, 649, 662, 880, 1063, 1444
Eam1104I CTCTTC 2 cut(s) 373, 1163
EarI CTCTTC 2 cut(s) 373, 1163
EciI GGCGGA 1 cut(s) 308
Ecl136II GAGCTC 1 cut(s) 1185
Eco130I CCWWGG 5 cut(s) 298, 521, 528, 899, 1133
Eco24I GRGCYC 2 cut(s) 1156, 1187
Eco31I GGTCTC 1 cut(s) 1049
Eco47I GGWCC 3 cut(s) 295, 518, 525
Eco47III AGCGCT 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 1185
Eco57I CTGAAG 2 cut(s) 107, 1071
Eco81I CCTNAGG 1 cut(s) 656
Eco88I CYCGRG 2 cut(s) 836, 912
EcoICRI GAGCTC 1 cut(s) 1185
EcoO109I RGGNCCY 1 cut(s) 1153
EcoRI GAATTC 3 cut(s) 818, 1220, 1380
EcoRII CCWGG 2 cut(s) 509, 956
EcoT14I CCWWGG 5 cut(s) 298, 521, 528, 899, 1133
EcoT22I ATGCAT 1 cut(s) 1092
EcoT38I GRGCYC 2 cut(s) 1156, 1187
ErhI CCWWGG 5 cut(s) 298, 521, 528, 899, 1133
FaeI CATG 6 cut(s) 274, 476, 870, 898, 1328, 1396
FaqI GGGAC 2 cut(s) 454, 1184
FatI CATG 6 cut(s) 270, 472, 866, 894, 1324, 1392
FbaI TGATCA 3 cut(s) 267, 649, 880
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 5 cut(s) 38, 59, 255, 429, 1322
FokI GGATG 3 cut(s) 207, 287, 769
FriOI GRGCYC 2 cut(s) 1156, 1187
Fsp4HI GCNGC 5 cut(s) 38, 59, 255, 429, 1322
FspBI CTAG 5 cut(s) 206, 536, 621, 1134, 1461
GlaI GCGC 2 cut(s) 210, 834
GluI GCNGC 5 cut(s) 38, 59, 255, 429, 1322
GsuI CTGGAG 2 cut(s) 264, 783
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 2 cut(s) 751, 1154
HapII CCGG 2 cut(s) 407, 936
HhaI GCGC 2 cut(s) 211, 835
Hin1II CATG 6 cut(s) 274, 476, 870, 898, 1328, 1396
Hin6I GCGC 2 cut(s) 209, 833
HinP1I GCGC 2 cut(s) 209, 833
HindIII AAGCTT 1 cut(s) 336
HpaII CCGG 2 cut(s) 407, 936
HphI GGTGA 5 cut(s) 644, 659, 946, 958, 998
Hpy166II GTNNAC 4 cut(s) 889, 928, 1035, 1363
Hpy188III TCNNGA 8 cut(s) 224, 407, 546, 762, 809, 974, 1162, 1393
Hpy8I GTNNAC 4 cut(s) 889, 928, 1035, 1363
HpyAV CCTTC 1 cut(s) 1132
HpyCH4III ACNGT 2 cut(s) 454, 1108
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 98, 254, 425, 1101, 1277, 1318
HpyF3I CTNAG 6 cut(s) 117, 135, 249, 312, 633, 656
Hsp92II CATG 6 cut(s) 274, 476, 870, 898, 1328, 1396
HspAI GCGC 2 cut(s) 209, 833
Kpn2I TCCGGA 1 cut(s) 406
Ksp22I TGATCA 3 cut(s) 267, 649, 880
Kzo9I GATC 8 cut(s) 164, 267, 532, 649, 662, 880, 1063, 1444
LguI GCTCTTC 1 cut(s) 373
LmnI GCTCC 5 cut(s) 77, 245, 672, 764, 1182
Lsp1109I GCAGC 4 cut(s) 49, 70, 241, 415
LweI GCATC 5 cut(s) 45, 265, 791, 1077, 1384
MaeI CTAG 5 cut(s) 206, 536, 621, 1134, 1461
MaeIII GTNAC 1 cut(s) 1463
MalI GATC 8 cut(s) 166, 269, 534, 651, 664, 882, 1065, 1446
MboI GATC 8 cut(s) 164, 267, 532, 649, 662, 880, 1063, 1444
MboII GAAGA 8 cut(s) 59, 360, 498, 582, 657, 1019, 1064, 1150
MfeI CAATTG 1 cut(s) 1038
MflI RGATCY 2 cut(s) 532, 662
MhlI GDGCHC 4 cut(s) 874, 930, 1156, 1187
MlyI GAGTC 3 cut(s) 419, 1109, 1309
MmeI TCCRAC 1 cut(s) 627
Mph1103I ATGCAT 1 cut(s) 1092
MroI TCCGGA 1 cut(s) 406
MroXI GAANNNNTTC 4 cut(s) 194, 307, 367, 574
MseI TTAA 8 cut(s) 63, 261, 737, 852, 918, 942, 1002, 1308
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 2 cut(s) 407, 936
MspR9I CCNGG 3 cut(s) 511, 936, 958
MunI CAATTG 1 cut(s) 1038
MvaI CCWGG 2 cut(s) 511, 958
MwoI GCNNNNNNNGC 7 cut(s) 29, 98, 254, 425, 1101, 1277, 1318
NciI CCSGG 1 cut(s) 936
NdeII GATC 8 cut(s) 164, 267, 532, 649, 662, 880, 1063, 1444
NlaIII CATG 6 cut(s) 274, 476, 870, 898, 1328, 1396
NlaIV GGNNCC 7 cut(s) 766, 807, 1105, 1154, 1261, 1405, 1432
NmuCI GTSAC 1 cut(s) 1463
NsiI ATGCAT 1 cut(s) 1092
NspI RCATGY 2 cut(s) 476, 898
NspV TTCGAA 1 cut(s) 1218
PaeI GCATGC 2 cut(s) 476, 898
PaeR7I CTCGAG 1 cut(s) 912
PagI TCATGA 1 cut(s) 1392
PciSI GCTCTTC 1 cut(s) 373
PdmI GAANNNNTTC 4 cut(s) 194, 307, 367, 574
PfeI GAWTC 7 cut(s) 403, 503, 587, 629, 682, 758, 1368
PfoI TCCNGGA 1 cut(s) 934
PkrI GCNGC 5 cut(s) 39, 60, 256, 430, 1323
PleI GAGTC 3 cut(s) 418, 1109, 1308
PpsI GAGTC 3 cut(s) 418, 1109, 1308
Psp124BI GAGCTC 1 cut(s) 1187
Psp6I CCWGG 2 cut(s) 509, 956
PspGI CCWGG 2 cut(s) 509, 956
PspN4I GGNNCC 7 cut(s) 766, 807, 1105, 1154, 1261, 1405, 1432
PspOMI GGGCCC 1 cut(s) 1152
PspPI GGNCC 5 cut(s) 295, 518, 525, 1152, 1153
PspXI VCTCGAGB 1 cut(s) 912
PsuI RGATCY 2 cut(s) 532, 662
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 4 cut(s) 375, 789, 1034, 1364
RsaNI GTAC 4 cut(s) 374, 788, 1033, 1363
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 1187
SapI GCTCTTC 1 cut(s) 373
SaqAI TTAA 8 cut(s) 63, 261, 737, 852, 918, 942, 1002, 1308
SatI GCNGC 5 cut(s) 38, 59, 255, 429, 1322
Sau3AI GATC 8 cut(s) 164, 267, 532, 649, 662, 880, 1063, 1444
Sau96I GGNCC 5 cut(s) 295, 518, 525, 1152, 1153
SchI GAGTC 3 cut(s) 419, 1109, 1309
ScrFI CCNGG 3 cut(s) 511, 936, 958
SduI GDGCHC 4 cut(s) 874, 930, 1156, 1187
SfaNI GCATC 5 cut(s) 45, 265, 791, 1077, 1384
Sfr274I CTCGAG 1 cut(s) 912
SfuI TTCGAA 1 cut(s) 1218
SinI GGWCC 3 cut(s) 295, 518, 525
SlaI CTCGAG 1 cut(s) 912
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 912
SmoI CTYRAG 1 cut(s) 912
SphI GCATGC 2 cut(s) 476, 898
SsiI CCGC 2 cut(s) 293, 1322
SspI AATATT 1 cut(s) 847
SspMI CTAG 5 cut(s) 206, 536, 621, 1134, 1461
SstI GAGCTC 1 cut(s) 1187
StyD4I CCNGG 3 cut(s) 509, 934, 956
StyI CCWWGG 5 cut(s) 298, 521, 528, 899, 1133
TaaI ACNGT 2 cut(s) 454, 1108
TaqI TCGA 5 cut(s) 545, 913, 1212, 1218, 1378
TatI WGTACW 2 cut(s) 1032, 1362
TauI GCSGC 1 cut(s) 1324
TfiI GAWTC 7 cut(s) 403, 503, 587, 629, 682, 758, 1368
Tru1I TTAA 8 cut(s) 63, 261, 737, 852, 918, 942, 1002, 1308
Tru9I TTAA 8 cut(s) 63, 261, 737, 852, 918, 942, 1002, 1308
TscAI CASTG 4 cut(s) 457, 598, 700, 1054
TseFI GTSAC 1 cut(s) 1463
TseI GCWGC 4 cut(s) 37, 58, 254, 428
Tsp45I GTSAC 1 cut(s) 1463
TspDTI ATGAA 5 cut(s) 209, 569, 737, 1326, 1381
TspGWI ACGGA 1 cut(s) 775
TspRI CASTG 4 cut(s) 457, 598, 700, 1054
VneI GTGCAC 1 cut(s) 926
VpaK11BI GGWCC 3 cut(s) 295, 518, 525
XapI RAATTY 5 cut(s) 236, 598, 818, 1220, 1380
XceI RCATGY 2 cut(s) 476, 898
XhoI CTCGAG 1 cut(s) 912
XmaJI CCTAGG 1 cut(s) 1133
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 4 cut(s) 194, 307, 367, 574
XspI CTAG 5 cut(s) 206, 536, 621, 1134, 1461
Zsp2I ATGCAT 1 cut(s) 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.