FvH4_6g04760

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000587
Physical Location & Seq
Forward (+)
2620592 .. 2627021
6430 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04760.t1

Sequence Viewer

Length: 1596 bp
ATGGACTGCAACAAAGAGGAGGCAATCAGGGCAATGCAGCTGTCACAAGAAAAGATGCAGATTAATGATTTTGCAGGAGCAAGGAAGATGGCACTAACAGCTCAATACCTCTTCCCTGAGCTTAAAAACATTTCTCAGTTACTAACTGCCTGTGAGGTTCACTGTTCAGCAGAAAACAAGATAGCTGGACTTGAAATGGACTGGTATGGGGTTCTTCAGATTCAACAGTTTGATAATGATGAAAGCATAAAGAAACAATTCAAAAAACTTGCACTATTACTTCATCCTGATAAGAATAAATTTCCTGGTGCTGAAGCTGCTTTTAAGCTGATTGTGGAAGCAAACAGGGTGCTGGCGGACCAAGTAACTCGTGCTAAGTATGACAAGAAGTATAGAAAAGCTTTAGCAAAAGCTAGTGAAATTGGAAGCTCTTCTGTACCCAAATATTACAAAGGAAAGGAAACCTCGAATCCAGAGTCAAAGGCAGGAATTGTTGCTGATGCTGGGCCTTCTAACACTGTAAAGAAAGATATTGAGCATGGAGATGGAGTGGGGAAGCAAGGTATTGATTCTTCTATGTCTGGTCCAAGGTCCAAAAGTCCAGGAACTTCCAGAAATATGAATAAAAAAAGAGAAGGGAGTTCAGGTTTTGAATCCATTCGAAGTAGCAAGGGAGGAAAGAGAAGGAGAGCTGATTCTGAACCGAATGGATGTGACTATCTTAGGAAATCTTCGAGGAGCAAGCAGAATCTTTCATCCGGTGAAAAGCTAAATAGTAATGGCTGTGTGCTGAATCCTCCAAAAAGTTCATTTAGTGCTTCCGAAAAGAAAATGAAAAATGCAGCTATGAAGTGTGGGATACGGAAAAATGATAGGTCAACTGATTCTGGAGCCAATTCAGTTGTTACAAACGTTCCAAAGGTCATTGATGTCCCTGATTCTGAATTCAATAAGTTTATTCTTGAGGAGAACAGTTTTAGTGCTAATCAAACATGGGCGCTTTATGATGAAGTAGATGGCATGCCAAGGTTCTATGCTCATGTCAAGAAGGTGCACTCTCCAGGATTTAAGGTGAAAATCACCTGGCTCATTCCCAACCCAGTTAACAAAGGTGAGAGGGCTTGGGTTAAGGAAGACCTGCCAGTTGCTTGTGGTAAGTTCAAACTTGGGCTCACTGAAGACATTACAGACCCTCTTGCATTTTCTCATCAGATGCATTGCACAAGAGGCAGTGTCAGAGGCCCTTTTCTGGTATATCCTAGGAAGGGAGAAACTTGGGCCCTCTTCAAGAATTGGGACATTGGATGGAGCTCTGACCCTGCAAAGCACAAGCCATTCGACTTTGAATTTGTGGAAGTCCTGTCAGATTTTTTGGAATCTGTTGGCACTGAAGTAGCTTATTTAGCCAAAGTCGAAGGCTTTTTGAGCATGTTCAAGCGAAACAAGCAGCATGGTGTCATTTCATTTCAGATACCAGCTCACGAGCTCTACAGATTCTCGCATCGAATCCCCTCATTCAAGATGTCTGGTGGCGAAAAGCGTGGTGTTCCGAAAGATTCCTTTGAGCTTGATCCGGCCTCTCTGCCCAGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

532

Amino Acids

59.13

Weight (kDa)

9.3

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 128 3e-18 DnaJ domain
DUF3444 PF11926 307 - 511 1.6e-74 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1146
AciI CCGC 1 cut(s) 356
AclI AACGTT 1 cut(s) 912
AclWI GGATC 1 cut(s) 1565
AcsI RAATTY 3 cut(s) 299, 944, 1346
AcuI CTGAAG 4 cut(s) 200, 333, 1197, 1410
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 2 cut(s) 1249, 1265
AjnI CCWGG 4 cut(s) 304, 601, 1060, 1082
Alw21I GWGCWC 3 cut(s) 1056, 1313, 1488
Alw44I GTGCAC 1 cut(s) 1052
AlwI GGATC 1 cut(s) 1565
AoxI GGCC 4 cut(s) 506, 1240, 1278, 1575
ApaI GGGCCC 1 cut(s) 1282
ApaLI GTGCAC 1 cut(s) 1052
ApeKI GCWGC 4 cut(s) 37, 317, 842, 1447
ApoI RAATTY 3 cut(s) 299, 944, 1346
ArsI GACNNNNNNTTYG 4 cut(s) 461, 493, 1331, 1363
AseI ATTAAT 1 cut(s) 63
Asp700I GAANNNNTTC 3 cut(s) 257, 430, 657
AspA2I CCTAGG 1 cut(s) 1259
AspLEI GCGC 1 cut(s) 1000
AspS9I GGNCC 7 cut(s) 358, 506, 584, 591, 1241, 1278, 1279
AsuHPI GGTGA 4 cut(s) 773, 1072, 1084, 1124
AsuII TTCGAA 1 cut(s) 661
AvaII GGWCC 3 cut(s) 358, 584, 591
AvrII CCTAGG 1 cut(s) 1259
BaeGI GKGCMC 2 cut(s) 1056, 1282
BanII GRGCYC 4 cut(s) 1173, 1282, 1313, 1488
BarI GAAGNNNNNNTAC 2 cut(s) 556, 588
BauI CACGAG 2 cut(s) 369, 1481
BbsI GAAGAC 2 cut(s) 1140, 1185
Bbv12I GWGCWC 3 cut(s) 1056, 1313, 1488
BbvI GCAGC 4 cut(s) 49, 304, 854, 1459
BccI CCATC 4 cut(s) 82, 539, 1010, 1299
BciT130I CCWGG 4 cut(s) 306, 603, 1062, 1084
BciVI GTATCC 1 cut(s) 852
BfaI CTAG 3 cut(s) 414, 1260, 1594
BfmI CTRYAG 1 cut(s) 1489
BfoI RGCGCY 1 cut(s) 1001
BfuAI ACCTGC 1 cut(s) 1146
BfuI GTATCC 1 cut(s) 852
BisI GCNGC 4 cut(s) 38, 318, 843, 1448
BlnI CCTAGG 1 cut(s) 1259
BlsI GCNGC 4 cut(s) 39, 319, 844, 1449
Bme1390I CCNGG 4 cut(s) 306, 603, 1062, 1084
Bme18I GGWCC 3 cut(s) 358, 584, 591
BmgT120I GGNCC 7 cut(s) 358, 506, 584, 591, 1241, 1278, 1279
BmiI GGNNCC 2 cut(s) 892, 1280
BmrFI CCNGG 4 cut(s) 306, 603, 1062, 1084
BmrI ACTGGG 2 cut(s) 1094, 1581
BmsI GCATC 4 cut(s) 45, 490, 1203, 1510
BmuI ACTGGG 2 cut(s) 1094, 1581
BpiI GAAGAC 2 cut(s) 1140, 1185
BpmI CTGGAG 2 cut(s) 909, 1044
Bpu10I CCTNAGC 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 661
BpuEI CTTGAG 1 cut(s) 983
BsaJI CCNNGG 3 cut(s) 587, 1025, 1259
BsaWI WCCGGW 1 cut(s) 758
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 631, 661
Bsc4I CCNNNNNNNGG 2 cut(s) 1249, 1265
Bse1I ACTGG 4 cut(s) 206, 1100, 1142, 1587
Bse3DI GCAATG 2 cut(s) 39, 1216
BseBI CCWGG 4 cut(s) 306, 603, 1062, 1084
BseDI CCNNGG 3 cut(s) 587, 1025, 1259
BseGI GGATG 4 cut(s) 283, 716, 755, 1310
BseLI CCNNNNNNNGG 2 cut(s) 1249, 1265
BseMI GCAATG 2 cut(s) 39, 1216
BseMII CTCAG 2 cut(s) 108, 149
BseNI ACTGG 4 cut(s) 206, 1100, 1142, 1587
BseRI GAGGAG 3 cut(s) 32, 751, 980
BseSI GKGCMC 2 cut(s) 1056, 1282
BseXI GCAGC 4 cut(s) 49, 304, 854, 1459
BseYI CCCAGC 1 cut(s) 503
BshFI GGCC 4 cut(s) 508, 1242, 1280, 1577
BsiHKAI GWGCWC 3 cut(s) 1056, 1313, 1488
BsiSI CCGG 2 cut(s) 759, 1574
BslFI GGGAC 2 cut(s) 917, 1310
BslI CCNNNNNNNGG 2 cut(s) 1249, 1265
BsmFI GGGAC 2 cut(s) 917, 1310
BsnI GGCC 4 cut(s) 508, 1242, 1280, 1577
Bsp119I TTCGAA 1 cut(s) 661
Bsp120I GGGCCC 1 cut(s) 1278
Bsp1286I GDGCHC 5 cut(s) 1056, 1173, 1282, 1313, 1488
Bsp143I GATC 1 cut(s) 1570
BspACI CCGC 1 cut(s) 356
BspANI GGCC 4 cut(s) 508, 1242, 1280, 1577
BspCNI CTCAG 2 cut(s) 109, 148
BspLI GGNNCC 2 cut(s) 892, 1280
BspMI ACCTGC 1 cut(s) 1146
BspPI GGATC 1 cut(s) 1565
BspQI GCTCTTC 1 cut(s) 436
BspT104I TTCGAA 1 cut(s) 661
BsrDI GCAATG 2 cut(s) 39, 1216
BsrI ACTGG 4 cut(s) 206, 1100, 1142, 1587
BssECI CCNNGG 3 cut(s) 587, 1025, 1259
BssMI GATC 1 cut(s) 1570
BssSI CACGAG 2 cut(s) 369, 1481
BssT1I CCWWGG 3 cut(s) 587, 1025, 1259
Bst2BI CACGAG 2 cut(s) 369, 1481
Bst2UI CCWGG 4 cut(s) 306, 603, 1062, 1084
Bst4CI ACNGT 4 cut(s) 164, 228, 520, 974
Bst6I CTCTTC 3 cut(s) 116, 436, 1289
BstBI TTCGAA 1 cut(s) 661
BstC8I GCNNGC 3 cut(s) 354, 743, 1022
BstDEI CTNAG 4 cut(s) 117, 135, 375, 722
BstF5I GGATG 4 cut(s) 283, 716, 755, 1310
BstH2I RGCGCY 1 cut(s) 1001
BstHHI GCGC 1 cut(s) 1000
BstKTI GATC 1 cut(s) 1573
BstMBI GATC 1 cut(s) 1570
BstMWI GCNNNNNNNGC 7 cut(s) 29, 98, 317, 1227, 1403, 1425, 1444
BstNI CCWGG 4 cut(s) 306, 603, 1062, 1084
BstNSI RCATGY 2 cut(s) 1024, 1432
BstSCI CCNGG 4 cut(s) 304, 601, 1060, 1082
BstSFI CTRYAG 1 cut(s) 1489
BstSLI GKGCMC 2 cut(s) 1056, 1282
BstV1I GCAGC 4 cut(s) 49, 304, 854, 1459
BstV2I GAAGAC 2 cut(s) 1140, 1185
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 4 cut(s) 508, 1242, 1280, 1577
BtsCI GGATG 4 cut(s) 283, 716, 755, 1310
BtsI GCAGTG 1 cut(s) 1237
BtsIMutI CAGTG 6 cut(s) 160, 516, 1173, 1237, 1386, 1594
BveI ACCTGC 1 cut(s) 1146
Cac8I GCNNGC 3 cut(s) 354, 743, 1022
CfoI GCGC 1 cut(s) 1000
Cfr13I GGNCC 7 cut(s) 358, 506, 584, 591, 1241, 1278, 1279
Csp6I GTAC 1 cut(s) 437
CviAII CATG 6 cut(s) 539, 993, 1021, 1040, 1429, 1451
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 4 cut(s) 117, 135, 375, 722
DpnI GATC 1 cut(s) 1572
DpnII GATC 1 cut(s) 1570
Eam1104I CTCTTC 3 cut(s) 116, 436, 1289
EarI CTCTTC 3 cut(s) 116, 436, 1289
EciI GGCGGA 1 cut(s) 371
Ecl136II GAGCTC 2 cut(s) 1311, 1486
Eco130I CCWWGG 3 cut(s) 587, 1025, 1259
Eco24I GRGCYC 4 cut(s) 1173, 1282, 1313, 1488
Eco47I GGWCC 3 cut(s) 358, 584, 591
Eco53kI GAGCTC 2 cut(s) 1311, 1486
Eco57I CTGAAG 4 cut(s) 200, 333, 1197, 1410
EcoICRI GAGCTC 2 cut(s) 1311, 1486
EcoO109I RGGNCCY 2 cut(s) 1241, 1279
EcoRI GAATTC 1 cut(s) 944
EcoRII CCWGG 4 cut(s) 304, 601, 1060, 1082
EcoT14I CCWWGG 3 cut(s) 587, 1025, 1259
EcoT22I ATGCAT 1 cut(s) 1218
EcoT38I GRGCYC 4 cut(s) 1173, 1282, 1313, 1488
ErhI CCWWGG 3 cut(s) 587, 1025, 1259
FaeI CATG 6 cut(s) 542, 996, 1024, 1043, 1432, 1454
FaqI GGGAC 2 cut(s) 917, 1310
FatI CATG 6 cut(s) 538, 992, 1020, 1039, 1428, 1450
Fnu4HI GCNGC 4 cut(s) 38, 318, 843, 1448
FokI GGATG 4 cut(s) 270, 723, 742, 1317
FriOI GRGCYC 4 cut(s) 1173, 1282, 1313, 1488
Fsp4HI GCNGC 4 cut(s) 38, 318, 843, 1448
FspBI CTAG 3 cut(s) 414, 1260, 1594
GlaI GCGC 1 cut(s) 999
GluI GCNGC 4 cut(s) 38, 318, 843, 1448
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 2 cut(s) 909, 1044
HaeII RGCGCY 1 cut(s) 1001
HaeIII GGCC 4 cut(s) 508, 1242, 1280, 1577
HapII CCGG 2 cut(s) 759, 1574
HhaI GCGC 1 cut(s) 1000
Hin1II CATG 6 cut(s) 542, 996, 1024, 1043, 1432, 1454
Hin6I GCGC 1 cut(s) 998
HinP1I GCGC 1 cut(s) 998
HincII GTYRAC 2 cut(s) 879, 1105
HindII GTYRAC 2 cut(s) 879, 1105
HindIII AAGCTT 1 cut(s) 399
HpaI GTTAAC 1 cut(s) 1105
HpaII CCGG 2 cut(s) 759, 1574
HphI GGTGA 4 cut(s) 773, 1072, 1084, 1124
Hpy166II GTNNAC 4 cut(s) 160, 879, 1054, 1105
Hpy188III TCNNGA 9 cut(s) 287, 473, 612, 888, 962, 1045, 1288, 1481, 1519
Hpy8I GTNNAC 4 cut(s) 160, 879, 1054, 1105
HpyAV CCTTC 6 cut(s) 519, 629, 678, 1042, 1258, 1409
HpyCH4III ACNGT 4 cut(s) 164, 228, 520, 974
HpyCH4IV ACGT 1 cut(s) 912
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 98, 317, 1227, 1403, 1425, 1444
HpyF3I CTNAG 4 cut(s) 117, 135, 375, 722
HpySE526I ACGT 1 cut(s) 912
Hsp92II CATG 6 cut(s) 542, 996, 1024, 1043, 1432, 1454
HspAI GCGC 1 cut(s) 998
KspAI GTTAAC 1 cut(s) 1105
Kzo9I GATC 1 cut(s) 1570
LguI GCTCTTC 1 cut(s) 436
LmnI GCTCC 4 cut(s) 77, 738, 890, 1308
Lsp1109I GCAGC 4 cut(s) 49, 304, 854, 1459
LweI GCATC 4 cut(s) 45, 490, 1203, 1510
MaeI CTAG 3 cut(s) 414, 1260, 1594
MaeII ACGT 1 cut(s) 912
MaeIII GTNAC 6 cut(s) 42, 138, 364, 713, 904, 1589
MalI GATC 1 cut(s) 1572
MboI GATC 1 cut(s) 1570
MboII GAAGA 9 cut(s) 97, 103, 206, 423, 564, 723, 1145, 1190, 1276
MhlI GDGCHC 5 cut(s) 1056, 1173, 1282, 1313, 1488
MluCI AATT 8 cut(s) 257, 299, 420, 489, 895, 944, 1291, 1346
MlyI GAGTC 1 cut(s) 485
Mph1103I ATGCAT 1 cut(s) 1218
MroXI GAANNNNTTC 3 cut(s) 257, 430, 657
MseI TTAA 6 cut(s) 63, 123, 324, 1068, 1104, 1128
MslI CAYNNNNRTG 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 2 cut(s) 759, 1574
MspR9I CCNGG 4 cut(s) 306, 603, 1062, 1084
MvaI CCWGG 4 cut(s) 306, 603, 1062, 1084
MwoI GCNNNNNNNGC 7 cut(s) 29, 98, 317, 1227, 1403, 1425, 1444
NdeII GATC 1 cut(s) 1570
NlaIII CATG 6 cut(s) 542, 996, 1024, 1043, 1432, 1454
NlaIV GGNNCC 2 cut(s) 892, 1280
NmuCI GTSAC 3 cut(s) 42, 713, 1589
NsiI ATGCAT 1 cut(s) 1218
NspI RCATGY 2 cut(s) 1024, 1432
NspV TTCGAA 1 cut(s) 661
PaeI GCATGC 1 cut(s) 1024
PciSI GCTCTTC 1 cut(s) 436
PdmI GAANNNNTTC 3 cut(s) 257, 430, 657
PfoI TCCNGGA 2 cut(s) 601, 1060
PkrI GCNGC 4 cut(s) 39, 319, 844, 1449
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PshBI ATTAAT 1 cut(s) 63
Psp124BI GAGCTC 2 cut(s) 1313, 1488
Psp1406I AACGTT 1 cut(s) 912
Psp6I CCWGG 4 cut(s) 304, 601, 1060, 1082
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 4 cut(s) 304, 601, 1060, 1082
PspN4I GGNNCC 2 cut(s) 892, 1280
PspOMI GGGCCC 1 cut(s) 1278
PspPI GGNCC 7 cut(s) 358, 506, 584, 591, 1241, 1278, 1279
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
RseI CAYNNNNRTG 1 cut(s) 543
SacI GAGCTC 2 cut(s) 1313, 1488
SapI GCTCTTC 1 cut(s) 436
SaqAI TTAA 6 cut(s) 63, 123, 324, 1068, 1104, 1128
SatI GCNGC 4 cut(s) 38, 318, 843, 1448
Sau3AI GATC 1 cut(s) 1570
Sau96I GGNCC 7 cut(s) 358, 506, 584, 591, 1241, 1278, 1279
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 4 cut(s) 306, 603, 1062, 1084
SduI GDGCHC 5 cut(s) 1056, 1173, 1282, 1313, 1488
SfaNI GCATC 4 cut(s) 45, 490, 1203, 1510
SfcI CTRYAG 1 cut(s) 1489
SfuI TTCGAA 1 cut(s) 661
SinI GGWCC 3 cut(s) 358, 584, 591
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 962
SmoI CTYRAG 1 cut(s) 962
SphI GCATGC 1 cut(s) 1024
Sse9I AATT 8 cut(s) 257, 299, 420, 489, 895, 944, 1291, 1346
SsiI CCGC 1 cut(s) 356
SspI AATATT 1 cut(s) 446
SspMI CTAG 3 cut(s) 414, 1260, 1594
SstI GAGCTC 2 cut(s) 1313, 1488
StyD4I CCNGG 4 cut(s) 304, 601, 1060, 1082
StyI CCWWGG 3 cut(s) 587, 1025, 1259
TaaI ACNGT 4 cut(s) 164, 228, 520, 974
TaiI ACGT 1 cut(s) 915
TaqI TCGA 6 cut(s) 467, 661, 734, 1338, 1413, 1504
TasI AATT 8 cut(s) 257, 299, 420, 489, 895, 944, 1291, 1346
Tru1I TTAA 6 cut(s) 63, 123, 324, 1068, 1104, 1128
Tru9I TTAA 6 cut(s) 63, 123, 324, 1068, 1104, 1128
TscAI CASTG 6 cut(s) 167, 523, 1180, 1237, 1393, 1594
TseFI GTSAC 3 cut(s) 42, 713, 1589
TseI GCWGC 4 cut(s) 37, 317, 842, 1447
Tsp45I GTSAC 3 cut(s) 42, 713, 1589
TspDTI ATGAA 9 cut(s) 255, 272, 635, 744, 798, 848, 863, 1023, 1452
TspGWI ACGGA 1 cut(s) 877
TspRI CASTG 6 cut(s) 167, 523, 1180, 1237, 1393, 1594
VneI GTGCAC 1 cut(s) 1052
VpaK11BI GGWCC 3 cut(s) 358, 584, 591
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 3 cut(s) 299, 944, 1346
XceI RCATGY 2 cut(s) 1024, 1432
XmaJI CCTAGG 1 cut(s) 1259
XmnI GAANNNNTTC 3 cut(s) 257, 430, 657
XspI CTAG 3 cut(s) 414, 1260, 1594
Zsp2I ATGCAT 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.