Domain of unknown function (DUF3444)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 217 |
| AciI | CCGC | 2 cut(s) | 320, 839 |
| AclWI | GGATC | 2 cut(s) | 1487, 2138 |
| AcsI | RAATTY | 4 cut(s) | 457, 1919, 2043, 2079 |
| AcuI | CTGAAG | 2 cut(s) | 1770, 1983 |
| AdeI | CACNNNGTG | 1 cut(s) | 521 |
| AfaI | GTAC | 2 cut(s) | 1601, 1747 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 516, 1838 |
| AjnI | CCWGG | 3 cut(s) | 124, 1441, 1595 |
| Alw21I | GWGCWC | 2 cut(s) | 409, 690 |
| Alw44I | GTGCAC | 1 cut(s) | 686 |
| AlwI | GGATC | 2 cut(s) | 1487, 2138 |
| Ama87I | CYCGRG | 3 cut(s) | 278, 509, 1633 |
| AoxI | GGCC | 3 cut(s) | 1102, 1445, 1851 |
| ApaI | GGGCCC | 1 cut(s) | 1855 |
| ApaLI | GTGCAC | 1 cut(s) | 686 |
| ApeKI | GCWGC | 7 cut(s) | 326, 485, 815, 848, 1244, 1247, 1304 |
| ApoI | RAATTY | 4 cut(s) | 457, 1919, 2043, 2079 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 815, 847, 1419, 1451 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 266, 2083 |
| AspA2I | CCTAGG | 1 cut(s) | 1832 |
| AspS9I | GGNCC | 6 cut(s) | 965, 1103, 1445, 1851, 1852, 2130 |
| AsuC2I | CCSGG | 2 cut(s) | 1634, 1635 |
| AsuHPI | GGTGA | 5 cut(s) | 1100, 1278, 1697, 1853, 2039 |
| AsuNHI | GCTAGC | 1 cut(s) | 524 |
| AvaI | CYCGRG | 3 cut(s) | 278, 509, 1633 |
| AvaII | GGWCC | 2 cut(s) | 965, 2130 |
| AvrII | CCTAGG | 1 cut(s) | 1832 |
| BaeGI | GKGCMC | 2 cut(s) | 690, 1855 |
| BanII | GRGCYC | 4 cut(s) | 409, 1208, 1567, 1855 |
| BbsI | GAAGAC | 1 cut(s) | 655 |
| Bbv12I | GWGCWC | 2 cut(s) | 409, 690 |
| BbvI | GCAGC | 7 cut(s) | 313, 472, 827, 835, 1234, 1256, 1291 |
| BccI | CCATC | 6 cut(s) | 91, 188, 854, 1307, 1580, 1872 |
| BciT130I | CCWGG | 3 cut(s) | 126, 1443, 1597 |
| BciVI | GTATCC | 1 cut(s) | 1839 |
| BcnI | CCSGG | 2 cut(s) | 1634, 1635 |
| BfaI | CTAG | 7 cut(s) | 191, 525, 692, 1041, 1355, 1833, 2182 |
| BfmI | CTRYAG | 1 cut(s) | 224 |
| BfuAI | ACCTGC | 1 cut(s) | 217 |
| BfuI | GTATCC | 1 cut(s) | 1839 |
| BisI | GCNGC | 7 cut(s) | 327, 486, 816, 849, 1245, 1248, 1305 |
| BlnI | CCTAGG | 1 cut(s) | 1832 |
| BlsI | GCNGC | 7 cut(s) | 328, 487, 817, 850, 1246, 1249, 1306 |
| BmcAI | AGTACT | 1 cut(s) | 1747 |
| Bme1390I | CCNGG | 5 cut(s) | 126, 1443, 1597, 1634, 1635 |
| Bme18I | GGWCC | 2 cut(s) | 965, 2130 |
| BmeT110I | CYCGRG | 3 cut(s) | 278, 509, 1633 |
| BmgT120I | GGNCC | 6 cut(s) | 965, 1103, 1445, 1851, 1852, 2130 |
| BmiI | GGNNCC | 5 cut(s) | 707, 1395, 1488, 1853, 2131 |
| BmrFI | CCNGG | 5 cut(s) | 126, 1443, 1597, 1634, 1635 |
| BmrI | ACTGGG | 3 cut(s) | 1398, 1876, 2109 |
| BmsI | GCATC | 6 cut(s) | 54, 472, 1291, 1472, 1654, 2083 |
| BmtI | GCTAGC | 1 cut(s) | 528 |
| BmuI | ACTGGG | 3 cut(s) | 1398, 1876, 2109 |
| BpiI | GAAGAC | 1 cut(s) | 655 |
| BpuMI | CCSGG | 2 cut(s) | 1634, 1635 |
| Bsa29I | ATCGAT | 1 cut(s) | 622 |
| BsaBI | GATNNNNATC | 2 cut(s) | 746, 1668 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 516, 1838 |
| Bse3DI | GCAATG | 2 cut(s) | 48, 1789 |
| Bse8I | GATNNNNATC | 2 cut(s) | 746, 1668 |
| BseBI | CCWGG | 3 cut(s) | 126, 1443, 1597 |
| BseCI | ATCGAT | 1 cut(s) | 622 |
| BseGI | GGATG | 5 cut(s) | 69, 1318, 1463, 1669, 1883 |
| BseJI | GATNNNNATC | 2 cut(s) | 746, 1668 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 516, 1838 |
| BseMI | GCAATG | 2 cut(s) | 48, 1789 |
| BseMII | CTCAG | 4 cut(s) | 404, 715, 1044, 1878 |
| BseRI | GAGGAG | 1 cut(s) | 41 |
| BseSI | GKGCMC | 2 cut(s) | 690, 1855 |
| BseXI | GCAGC | 7 cut(s) | 313, 472, 827, 835, 1234, 1256, 1291 |
| BseYI | CCCAGC | 1 cut(s) | 1370 |
| BsgI | GTGCAG | 1 cut(s) | 550 |
| Bsh1285I | CGRYCG | 1 cut(s) | 370 |
| BshFI | GGCC | 3 cut(s) | 1104, 1447, 1853 |
| BshVI | ATCGAT | 1 cut(s) | 622 |
| BsiEI | CGRYCG | 1 cut(s) | 370 |
| BsiHKAI | GWGCWC | 2 cut(s) | 409, 690 |
| BsiHKCI | CYCGRG | 3 cut(s) | 278, 509, 1633 |
| BsiSI | CCGG | 1 cut(s) | 1634 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 516, 1838 |
| BsmI | GAATGC | 1 cut(s) | 1246 |
| BsnI | GGCC | 3 cut(s) | 1104, 1447, 1853 |
| BsoBI | CYCGRG | 3 cut(s) | 278, 509, 1633 |
| Bsp120I | GGGCCC | 1 cut(s) | 1851 |
| Bsp1286I | GDGCHC | 5 cut(s) | 409, 690, 1208, 1567, 1855 |
| Bsp143I | GATC | 5 cut(s) | 339, 439, 1492, 1762, 2143 |
| BspACI | CCGC | 2 cut(s) | 320, 839 |
| BspANI | GGCC | 3 cut(s) | 1104, 1447, 1853 |
| BspCNI | CTCAG | 4 cut(s) | 405, 714, 1045, 1879 |
| BspDI | ATCGAT | 1 cut(s) | 622 |
| BspHI | TCATGA | 1 cut(s) | 244 |
| BspLI | GGNNCC | 5 cut(s) | 707, 1395, 1488, 1853, 2131 |
| BspMAI | CTGCAG | 1 cut(s) | 228 |
| BspMI | ACCTGC | 1 cut(s) | 217 |
| BspOI | GCTAGC | 1 cut(s) | 528 |
| BspPI | GGATC | 2 cut(s) | 1487, 2138 |
| BspQI | GCTCTTC | 2 cut(s) | 414, 1196 |
| BsrDI | GCAATG | 2 cut(s) | 48, 1789 |
| BssMI | GATC | 5 cut(s) | 339, 439, 1492, 1762, 2143 |
| BssT1I | CCWWGG | 2 cut(s) | 954, 1832 |
| Bst2UI | CCWGG | 3 cut(s) | 126, 1443, 1597 |
| Bst4CI | ACNGT | 4 cut(s) | 479, 519, 592, 1212 |
| Bst6I | CTCTTC | 3 cut(s) | 125, 414, 1196 |
| BstC8I | GCNNGC | 2 cut(s) | 526, 1592 |
| BstDEI | CTNAG | 4 cut(s) | 413, 701, 1053, 1887 |
| BstEII | GGTNACC | 1 cut(s) | 1284 |
| BstF5I | GGATG | 5 cut(s) | 69, 1318, 1463, 1669, 1883 |
| BstKTI | GATC | 5 cut(s) | 342, 442, 1495, 1765, 2146 |
| BstMBI | GATC | 5 cut(s) | 339, 439, 1492, 1762, 2143 |
| BstMCI | CGRYCG | 1 cut(s) | 370 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 38, 107, 326, 845, 1304, 1800, 1897, 1976 |
| BstNI | CCWGG | 3 cut(s) | 126, 1443, 1597 |
| BstNSI | RCATGY | 2 cut(s) | 1061, 1594 |
| BstPI | GGTNACC | 1 cut(s) | 1284 |
| BstSCI | CCNGG | 5 cut(s) | 124, 1441, 1595, 1632, 1633 |
| BstSFI | CTRYAG | 1 cut(s) | 224 |
| BstSLI | GKGCMC | 2 cut(s) | 690, 1855 |
| BstV1I | GCAGC | 7 cut(s) | 313, 472, 827, 835, 1234, 1256, 1291 |
| BstV2I | GAAGAC | 1 cut(s) | 655 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 314 |
| Bsu15I | ATCGAT | 1 cut(s) | 622 |
| BsuI | GTATCC | 1 cut(s) | 1839 |
| BsuRI | GGCC | 3 cut(s) | 1104, 1447, 1853 |
| BsuTUI | ATCGAT | 1 cut(s) | 622 |
| BtsCI | GGATG | 5 cut(s) | 69, 1318, 1463, 1669, 1883 |
| BtsI | GCAGTG | 2 cut(s) | 205, 363 |
| BtsIMutI | CAGTG | 7 cut(s) | 205, 363, 475, 597, 951, 1208, 1222 |
| BveI | ACCTGC | 1 cut(s) | 217 |
| Cac8I | GCNNGC | 2 cut(s) | 526, 1592 |
| CciI | TCATGA | 1 cut(s) | 244 |
| Cfr13I | GGNCC | 6 cut(s) | 965, 1103, 1445, 1851, 1852, 2130 |
| Cfr9I | CCCGGG | 1 cut(s) | 1633 |
| ClaI | ATCGAT | 1 cut(s) | 622 |
| Csp6I | GTAC | 2 cut(s) | 1600, 1746 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2149, 2184 |
| CviAII | CATG | 9 cut(s) | 13, 245, 394, 529, 767, 896, 1058, 1560, 1591 |
| CviQI | GTAC | 2 cut(s) | 1600, 1746 |
| DdeI | CTNAG | 4 cut(s) | 413, 701, 1053, 1887 |
| DpnI | GATC | 5 cut(s) | 341, 441, 1494, 1764, 2145 |
| DpnII | GATC | 5 cut(s) | 339, 439, 1492, 1762, 2143 |
| DraI | TTTAAA | 1 cut(s) | 1546 |
| DraIII | CACNNNGTG | 1 cut(s) | 521 |
| Eam1104I | CTCTTC | 3 cut(s) | 125, 414, 1196 |
| EarI | CTCTTC | 3 cut(s) | 125, 414, 1196 |
| Ecl136II | GAGCTC | 1 cut(s) | 407 |
| Eco130I | CCWWGG | 2 cut(s) | 954, 1832 |
| Eco24I | GRGCYC | 4 cut(s) | 409, 1208, 1567, 1855 |
| Eco32I | GATATC | 1 cut(s) | 2032 |
| Eco47I | GGWCC | 2 cut(s) | 965, 2130 |
| Eco53kI | GAGCTC | 1 cut(s) | 407 |
| Eco57I | CTGAAG | 2 cut(s) | 1770, 1983 |
| Eco88I | CYCGRG | 3 cut(s) | 278, 509, 1633 |
| Eco91I | GGTNACC | 1 cut(s) | 1284 |
| EcoICRI | GAGCTC | 1 cut(s) | 407 |
| EcoO109I | RGGNCCY | 4 cut(s) | 965, 1103, 1852, 2130 |
| EcoO65I | GGTNACC | 1 cut(s) | 1284 |
| EcoRI | GAATTC | 2 cut(s) | 2043, 2079 |
| EcoRII | CCWGG | 3 cut(s) | 124, 1441, 1595 |
| EcoRV | GATATC | 1 cut(s) | 2032 |
| EcoT14I | CCWWGG | 2 cut(s) | 954, 1832 |
| EcoT38I | GRGCYC | 4 cut(s) | 409, 1208, 1567, 1855 |
| ErhI | CCWWGG | 2 cut(s) | 954, 1832 |
| FaeI | CATG | 9 cut(s) | 16, 248, 397, 532, 770, 899, 1061, 1563, 1594 |
| FatI | CATG | 9 cut(s) | 12, 244, 393, 528, 766, 895, 1057, 1559, 1590 |
| FauI | CCCGC | 1 cut(s) | 832 |
| Fnu4HI | GCNGC | 7 cut(s) | 327, 486, 816, 849, 1245, 1248, 1305 |
| FokI | GGATG | 5 cut(s) | 76, 1325, 1450, 1676, 1890 |
| FriOI | GRGCYC | 4 cut(s) | 409, 1208, 1567, 1855 |
| Fsp4HI | GCNGC | 7 cut(s) | 327, 486, 816, 849, 1245, 1248, 1305 |
| FspBI | CTAG | 7 cut(s) | 191, 525, 692, 1041, 1355, 1833, 2182 |
| GluI | GCNGC | 7 cut(s) | 327, 486, 816, 849, 1245, 1248, 1305 |
| GsaI | CCCAGC | 1 cut(s) | 1374 |
| HaeIII | GGCC | 3 cut(s) | 1104, 1447, 1853 |
| HapII | CCGG | 1 cut(s) | 1634 |
| Hin1II | CATG | 9 cut(s) | 16, 248, 397, 532, 770, 899, 1061, 1563, 1594 |
| HindIII | AAGCTT | 1 cut(s) | 1345 |
| HinfI | GANTC | 7 cut(s) | 220, 512, 611, 742, 959, 1007, 1049 |
| HpaII | CCGG | 1 cut(s) | 1634 |
| HphI | GGTGA | 5 cut(s) | 1100, 1278, 1697, 1853, 2039 |
| Hpy166II | GTNNAC | 2 cut(s) | 688, 1180 |
| Hpy8I | GTNNAC | 2 cut(s) | 688, 1180 |
| HpyAV | CCTTC | 9 cut(s) | 54, 100, 544, 907, 978, 1095, 1321, 1831, 2094 |
| HpyCH4III | ACNGT | 4 cut(s) | 479, 519, 592, 1212 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 38, 107, 326, 845, 1304, 1800, 1897, 1976 |
| HpyF3I | CTNAG | 4 cut(s) | 413, 701, 1053, 1887 |
| Hsp92II | CATG | 9 cut(s) | 16, 248, 397, 532, 770, 899, 1061, 1563, 1594 |
| Kzo9I | GATC | 5 cut(s) | 339, 439, 1492, 1762, 2143 |
| LguI | GCTCTTC | 2 cut(s) | 414, 1196 |
| LmnI | GCTCC | 2 cut(s) | 86, 863 |
| Lsp1109I | GCAGC | 7 cut(s) | 313, 472, 827, 835, 1234, 1256, 1291 |
| LweI | GCATC | 6 cut(s) | 54, 472, 1291, 1472, 1654, 2083 |
| MaeI | CTAG | 7 cut(s) | 191, 525, 692, 1041, 1355, 1833, 2182 |
| MaeIII | GTNAC | 6 cut(s) | 285, 513, 627, 790, 1284, 1435 |
| MalI | GATC | 5 cut(s) | 341, 441, 1494, 1764, 2145 |
| MboI | GATC | 5 cut(s) | 339, 439, 1492, 1762, 2143 |
| MfeI | CAATTG | 1 cut(s) | 1002 |
| MhlI | GDGCHC | 5 cut(s) | 409, 690, 1208, 1567, 1855 |
| MlyI | GAGTC | 3 cut(s) | 521, 605, 968 |
| MmeI | TCCRAC | 1 cut(s) | 994 |
| MroXI | GAANNNNTTC | 2 cut(s) | 266, 2083 |
| MseI | TTAA | 8 cut(s) | 291, 333, 450, 461, 1272, 1518, 1545, 1641 |
| MslI | CAYNNNNRTG | 1 cut(s) | 480 |
| MspA1I | CMGCKG | 2 cut(s) | 179, 1247 |
| MspI | CCGG | 1 cut(s) | 1634 |
| MspR9I | CCNGG | 5 cut(s) | 126, 1443, 1597, 1634, 1635 |
| MunI | CAATTG | 1 cut(s) | 1002 |
| Mva1269I | GAATGC | 1 cut(s) | 1246 |
| MvaI | CCWGG | 3 cut(s) | 126, 1443, 1597 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 38, 107, 326, 845, 1304, 1800, 1897, 1976 |
| NciI | CCSGG | 2 cut(s) | 1634, 1635 |
| NdeII | GATC | 5 cut(s) | 339, 439, 1492, 1762, 2143 |
| NheI | GCTAGC | 1 cut(s) | 524 |
| NlaIII | CATG | 9 cut(s) | 16, 248, 397, 532, 770, 899, 1061, 1563, 1594 |
| NlaIV | GGNNCC | 5 cut(s) | 707, 1395, 1488, 1853, 2131 |
| NmeAIII | GCCGAG | 1 cut(s) | 593 |
| NmuCI | GTSAC | 3 cut(s) | 513, 1284, 1435 |
| NspI | RCATGY | 2 cut(s) | 1061, 1594 |
| PaeI | GCATGC | 1 cut(s) | 1594 |
| PagI | TCATGA | 1 cut(s) | 244 |
| PasI | CCCWGGG | 1 cut(s) | 125 |
| PciSI | GCTCTTC | 2 cut(s) | 414, 1196 |
| PctI | GAATGC | 1 cut(s) | 1246 |
| PdmI | GAANNNNTTC | 2 cut(s) | 266, 2083 |
| PfeI | GAWTC | 4 cut(s) | 220, 742, 1007, 1049 |
| PkrI | GCNGC | 7 cut(s) | 328, 487, 817, 850, 1246, 1249, 1306 |
| PleI | GAGTC | 3 cut(s) | 520, 605, 967 |
| PpsI | GAGTC | 3 cut(s) | 520, 605, 967 |
| PpuMI | RGGWCCY | 2 cut(s) | 965, 2130 |
| Psp124BI | GAGCTC | 1 cut(s) | 409 |
| Psp5II | RGGWCCY | 2 cut(s) | 965, 2130 |
| Psp6I | CCWGG | 3 cut(s) | 124, 1441, 1595 |
| PspEI | GGTNACC | 1 cut(s) | 1284 |
| PspFI | CCCAGC | 1 cut(s) | 1370 |
| PspGI | CCWGG | 3 cut(s) | 124, 1441, 1595 |
| PspN4I | GGNNCC | 5 cut(s) | 707, 1395, 1488, 1853, 2131 |
| PspOMI | GGGCCC | 1 cut(s) | 1851 |
| PspPI | GGNCC | 6 cut(s) | 965, 1103, 1445, 1851, 1852, 2130 |
| PspPPI | RGGWCCY | 2 cut(s) | 965, 2130 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 150, 182, 1130, 1162 |
| PstI | CTGCAG | 1 cut(s) | 228 |
| PvuII | CAGCTG | 2 cut(s) | 179, 1247 |
| RsaI | GTAC | 2 cut(s) | 1601, 1747 |
| RsaNI | GTAC | 2 cut(s) | 1600, 1746 |
| RseI | CAYNNNNRTG | 1 cut(s) | 480 |
| SacI | GAGCTC | 1 cut(s) | 409 |
| SapI | GCTCTTC | 2 cut(s) | 414, 1196 |
| SaqAI | TTAA | 8 cut(s) | 291, 333, 450, 461, 1272, 1518, 1545, 1641 |
| SatI | GCNGC | 7 cut(s) | 327, 486, 816, 849, 1245, 1248, 1305 |
| Sau3AI | GATC | 5 cut(s) | 339, 439, 1492, 1762, 2143 |
| Sau96I | GGNCC | 6 cut(s) | 965, 1103, 1445, 1851, 1852, 2130 |
| ScaI | AGTACT | 1 cut(s) | 1747 |
| SchI | GAGTC | 3 cut(s) | 521, 605, 968 |
| ScrFI | CCNGG | 5 cut(s) | 126, 1443, 1597, 1634, 1635 |
| SduI | GDGCHC | 5 cut(s) | 409, 690, 1208, 1567, 1855 |
| SfaNI | GCATC | 6 cut(s) | 54, 472, 1291, 1472, 1654, 2083 |
| SfcI | CTRYAG | 1 cut(s) | 224 |
| SinI | GGWCC | 2 cut(s) | 965, 2130 |
| SmaI | CCCGGG | 1 cut(s) | 1635 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 480 |
| SphI | GCATGC | 1 cut(s) | 1594 |
| SsiI | CCGC | 2 cut(s) | 320, 839 |
| SspI | AATATT | 3 cut(s) | 139, 505, 1276 |
| SspMI | CTAG | 7 cut(s) | 191, 525, 692, 1041, 1355, 1833, 2182 |
| SstI | GAGCTC | 1 cut(s) | 409 |
| StyD4I | CCNGG | 5 cut(s) | 124, 1441, 1595, 1632, 1633 |
| StyI | CCWWGG | 2 cut(s) | 954, 1832 |
| TaaI | ACNGT | 4 cut(s) | 479, 519, 592, 1212 |
| TaqI | TCGA | 6 cut(s) | 622, 646, 971, 1124, 2041, 2077 |
| TaqII | GACCGA | 1 cut(s) | 384 |
| TatI | WGTACW | 1 cut(s) | 1745 |
| TfiI | GAWTC | 4 cut(s) | 220, 742, 1007, 1049 |
| Tru1I | TTAA | 8 cut(s) | 291, 333, 450, 461, 1272, 1518, 1545, 1641 |
| Tru9I | TTAA | 8 cut(s) | 291, 333, 450, 461, 1272, 1518, 1545, 1641 |
| TscAI | CASTG | 7 cut(s) | 205, 363, 482, 597, 951, 1215, 1222 |
| TseFI | GTSAC | 3 cut(s) | 513, 1284, 1435 |
| TseI | GCWGC | 7 cut(s) | 326, 485, 815, 848, 1244, 1247, 1304 |
| Tsp45I | GTSAC | 3 cut(s) | 513, 1284, 1435 |
| TspMI | CCCGGG | 1 cut(s) | 1633 |
| TspRI | CASTG | 7 cut(s) | 205, 363, 482, 597, 951, 1215, 1222 |
| VneI | GTGCAC | 1 cut(s) | 686 |
| VpaK11BI | GGWCC | 2 cut(s) | 965, 2130 |
| XapI | RAATTY | 4 cut(s) | 457, 1919, 2043, 2079 |
| XbaI | TCTAGA | 1 cut(s) | 1354 |
| XceI | RCATGY | 2 cut(s) | 1061, 1594 |
| XmaI | CCCGGG | 1 cut(s) | 1633 |
| XmaJI | CCTAGG | 1 cut(s) | 1832 |
| XmnI | GAANNNNTTC | 2 cut(s) | 266, 2083 |
| XspI | CTAG | 7 cut(s) | 191, 525, 692, 1041, 1355, 1833, 2182 |
| ZrmI | AGTACT | 1 cut(s) | 1747 |