Rh3AG050000

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000587
Physical Location & Seq
Forward (+)
3474936 .. 3480871
5936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG050000.1

Sequence Viewer

Length: 3306 bp
ATGGAGTGCAACAAAGAGGAGGCGGTCAGAGCAATGCAACTTTCAGAAACAAAGATGCAAAACAAAGATTATATAGGAGCAATGAAGACGGCACAAAAGGCTCAGAGACTCTTCCCTGATCTCGAAAACATTTCTCGGTTACTTACGGTATGTGAAGTTCACTGTACAGCAGAAAACAAGCTAGGTGGATCCGAAATGGATTGGTACGGAATTCTTCAGATTCAACAATTTGATAATGATGTCACCATCAAGAAACAGTATAGAAAATTGGCACTTTTGCTTCATCCTGATAAGAATAAGTTTGCTGGTTCGGAGGCTGCTTTTAAGCTGATTGGGGAAGCAAACAGGGTGCTGTCGGACAAACAAAAACGTTCTATATATGACATTAAGTATAGAACTTTAGCAAGACCTGGTGGATCAAAAACAACAGCTCATCAGTCAAATATTGATTTCTTTGCTGCAACCAATTCTCGGAATAATCCTCAGTCACCTGTGCAACCAAACACATTCTGGACACAGTGCTACTATTGTTTCTCAAAGTTTCAATACTATAGAGACTTTCTGGGTCGGTTGCTCCGTTGTCAAAGATGCCGGAAAGCATTTGAAGCTCATGAATTTCGGGAAGGTGTGCATCCAGAATCTTTCAGAAATGACTTTCCGAATCATAAAGAGCCGCCAAGTCAGGGTCCTTCAAACGTAGCCTCACAAAGTAATGGAGGAACTGAAAAAGGAAATTTCACAAGATTTCAGAATGGAGATGCAGCCTTCAAACCTGCGTCAAAGGTAGGAACTTCTGCTGACTTGAGTGGGGCTTCTGACGCTGAAAAGAAAGATATTTGGCGTGGGGTTGAAGTGGGGAAGCAAGGTGTTGAAACATCTAAATTTGGTCCAGTGAAGTCCAAAGATACAATAACTTCTAGAAATATGAACAAAGAAAGAGAGAATTCAACTTTTGAATTTAGAGAAAGTTTGAAGACTGGAAATAGAGCTACAGCTGATACTGAACGTGCTGCTAAAAAGAAGAATGATAGTGTTTCTGAACTCGGTGGAGGCCGTCACCCTAGGAGATCTCCAAGGAACAAGCATAATCTTTCATCCCTCCTAGATGACAATGATGATGATGATTTTGTGAGTCCCCCATTCAAAAGGTTGAGGAAGGGCCAATTGTTTAGTGCTGCTGAGGAGGGAAATAATGCAACTGTTGGTGGACATAAGAAAAATACAAGAGAAAAAGTAAGTTTTCCTCTAGAAGAAAATTTGCCAAAGAAGAAGAGTAAAACTGAGGAATTTGAGTTAACGGTAAAGGGAACCGCCATGTCAGATCATGATGAAAGCAACTTCAAAGGTGATTTCAGTCCTGTAGCTAATGTAGATGTTGCATCCACTCCGGGAGCCATTGAAGTTCCTGATCCTCAATTCCATAAGTTTGTGCTTGATGAAGATAGTTTGCCAAGTCTTTTTGAAGCCAATCAAATATGGGCTCTTTATGATCCAGCAGATGGCATGCCCAGATACTATGCTTTTGTCAAGAAGGTCCTCACCCCTGGATTTAAGCTGAAAATCAGATGGCTGGAGGCCAATCCAGATGACCAAGGTGAGATTGATTGGTCCGAGATGGAGTTGCCAGTTGCTTGTGGCAAGTTCAAACTTGGGAAAACTCAAGAAATTAAAGATCATCATATGTTTTCTCATCAGATGCACTACAGAAAAGGAAGTGATAAAAAGTCTATTCTGGTATATCCTAGGAAGGGAGAAACTTGGGCCATGTACCAGAATTGGGATATTGGATGGAGTTCTGAGCCGGAAAAGCACATGCCCTACAAATATGAATTTGTTGAAGTCTTGTCTGATTTTGTCGAAGCTTTTGGAATTGGAGTTCGTTATTTAGGCAAAGTGAAAGGATTTGTCAGTTTATTTCAGCCAACTGAGCAGCACGGGGTTGTCATGTTTCAAGTACCAACTCATGAGCTATACAGATTTTCTCATCAGATTCCCTCTTTCAAGATGACTGGTTGTGAAGGATCTGGTGTTCCCCCAGGATCCTTTGAGCTTGATCCTGCTTCTTTGCCCACCAGTATTTTTGATTCAAGTGATCTAGGTAACTTGGAAATGGATGACAGAAGCAAGAAAACTGAAACTGATGGTACGTCTTGTGAAAGGGTTGGTTCTGTAACTGCATGCACAGGTATGAAGCAGGAGAAGAAGCTTTCTGAAAGAGAGACTTTGATGTCTAGATCTCCAAGAGAGTCAGATACTGAATTTGTGAATTGTATGAAAGGGAAGCGCATGACAAACCTAAACCATGGAAACCTCACACAACCTGAGGAAAGTGCTATTCCATGTCAAGCAGATAAGAGAACCGACACACAGAAGCATCAAAAGAACAATTTGGATAAAGAAAGTTTTAGTCTTAGAAGATCACCAAGGGAATTAAGCAAGAACAGTACCCCATCAAACGGAGCTATGAAGTGTCCAGATTCTGCCAAGGACGACGGCCATGTCAGCTTCCCTCAGTCTAAAGTAAACTCTACCTCTAGCCAGTATAATAACAGGATTCATTCAACTCTGAAGGATCACCATACTCCCAGTCTCATGAAAAACCCTGTAGCCCCTCCTCCATCTTCATCACCTGCCTGCAGATTGTCCCAGACTGAATTCTACGACTTCAAAGGGCTAAAATCTCAGGAGAAATTTAGTCTTGGTCAAATTTGGGCTCTGTATAGTGAACGACATGGGATGCCTAATACTTATGCTCAAGTTAAGAGGATTGAGGTTAGGCCAAATTTTCAAGTGCATATGGCAGTGCTGGAACCTTGCTCAGAACCGGAAAATTTGTCTGGACCAGTTTCCTGTGGGACATTTAGATTGAAAAATTGCCCAACTGAGGTCTTCCCCCTCTCTTCATTTTCTCACTGCTTAAATACCAGAAATGTTGTTGGTAGGAAGGTGTTTGAAATACAACCCAAGGAAGGTGAGGTTTGGGCTCTATACAAGAACCACAATCCTGAGCCGACTTGTCCCAACCTGGCCAAGGGTGAATGTGAAATAGTGGAAGTGCGGGGAAATAGTGGAAGAAGCACAAAGGTTGGAGTTCTGGTAAAGGTTGAAGGATTTAAATCAATATTCAAGGCTCCAGGAATCCAGAGATCAAAAACTGGGATCATTGATGTACCGCGGTCTGAGTTTCATAGATTTTCTCACCAGATTCCTGCTTTCCAGCATAGCGGGGAGAGTGACAGTCGACTTGCAGGCTGTTGGGAGCTTGATCCATCATCAATACCTGGTACTGTAATTTCCTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

1101

Amino Acids

123.91

Weight (kDa)

8.61

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 128 8e-20 DnaJ domain
DUF3444 PF11926 465 - 672 2.7e-78 Domain of unknown function (DUF3444)
DUF3444 PF11926 880 - 1077 6.9e-49 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2638
AasI GACNNNNNNGTC 2 cut(s) 2227, 2498
Acc36I ACCTGC 2 cut(s) 781, 2638
AccB7I CCANNNNNTGG 1 cut(s) 1499
AccI GTMKAC 1 cut(s) 3241
AccII CGCG 1 cut(s) 3175
AciI CCGC 7 cut(s) 23, 674, 1311, 3058, 3173, 3175, 3225
AclI AACGTT 1 cut(s) 370
AcoI YGGCCR 2 cut(s) 2494, 3027
AcuI CTGAAG 2 cut(s) 200, 2588
AfaI GTAC 8 cut(s) 166, 206, 1769, 1956, 2146, 2446, 3171, 3286
AfiI CCNNNNNNNGG 9 cut(s) 471, 683, 1499, 1748, 1777, 2456, 2884, 2969, 3031
AjnI CCWGG 6 cut(s) 409, 1543, 2035, 3024, 3133, 3280
AloI GAACNNNNNNTCC 4 cut(s) 1860, 1892, 2013, 2045
Alw26I GTCTC 4 cut(s) 100, 549, 2213, 2594
AlwNI CAGNNNCTG 2 cut(s) 2255, 2480
AoxI GGCC 7 cut(s) 1051, 1159, 1575, 1761, 2494, 2777, 3027
ApeKI GCWGC 6 cut(s) 317, 458, 761, 1010, 1175, 1930
AspA2I CCTAGG 2 cut(s) 1061, 1742
AspLEI GCGC 1 cut(s) 2286
AspS9I GGNCC 7 cut(s) 686, 887, 1159, 1534, 1608, 1761, 2840
AsuC2I CCSGG 1 cut(s) 1389
AvaII GGWCC 5 cut(s) 686, 887, 1534, 1608, 2840
AvrII CCTAGG 2 cut(s) 1061, 1742
AxyI CCTNAGG 1 cut(s) 2322
BalI TGGCCA 1 cut(s) 3029
BamHI GGATCC 2 cut(s) 188, 2039
BanII GRGCYC 3 cut(s) 1483, 2716, 2986
BbsI GAAGAC 3 cut(s) 92, 980, 2881
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 6 cut(s) 304, 445, 773, 997, 1162, 1942
BccI CCATC 9 cut(s) 254, 1493, 1560, 1609, 1782, 2135, 2458, 2626, 3277
BceAI ACGGC 3 cut(s) 105, 1038, 2509
BciT130I CCWGG 6 cut(s) 411, 1545, 2037, 3026, 3135, 3282
BcnI CCSGG 1 cut(s) 1389
BcoDI GTCTC 4 cut(s) 100, 549, 2213, 2594
BfaI CTAG 9 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096, 2232, 2535
BfmI CTRYAG 6 cut(s) 550, 990, 1359, 1702, 2604, 2635
BfuAI ACCTGC 2 cut(s) 781, 2638
BglII AGATCT 2 cut(s) 1067, 2234
BisI GCNGC 7 cut(s) 318, 459, 674, 762, 1011, 1176, 1931
BlnI CCTAGG 2 cut(s) 1061, 1742
BlsI GCNGC 7 cut(s) 319, 460, 675, 763, 1012, 1177, 1932
Bme1390I CCNGG 7 cut(s) 411, 1389, 1545, 2037, 3026, 3135, 3282
Bme18I GGWCC 5 cut(s) 686, 887, 1534, 1608, 2840
BmgT120I GGNCC 7 cut(s) 686, 887, 1159, 1534, 1608, 1761, 2840
BmiI GGNNCC 7 cut(s) 190, 687, 1309, 1393, 2041, 2811, 3132
BmrFI CCNGG 7 cut(s) 411, 1389, 1545, 2037, 3026, 3135, 3282
BmrI ACTGGG 2 cut(s) 2580, 3165
BmsI GCATC 8 cut(s) 45, 578, 640, 748, 1388, 1686, 2383, 2727
BmuI ACTGGG 2 cut(s) 2580, 3165
BpiI GAAGAC 3 cut(s) 92, 980, 2881
BpmI CTGGAG 2 cut(s) 1592, 3117
Bpu10I CCTNAGC 2 cut(s) 1179, 3006
BpuEI CTTGAG 3 cut(s) 823, 1644, 2739
BpuMI CCSGG 1 cut(s) 1389
BsaBI GATNNNNATC 1 cut(s) 3115
BsaWI WCCGGW 1 cut(s) 2824
BsaXI ACNNNNNCTCC 2 cut(s) 3081, 3111
Bsc4I CCNNNNNNNGG 9 cut(s) 471, 683, 1499, 1748, 1777, 2456, 2884, 2969, 3031
Bse1I ACTGG 9 cut(s) 890, 982, 1625, 2014, 2073, 2539, 2586, 2843, 3160
Bse21I CCTNAGG 1 cut(s) 2322
Bse3DI GCAATG 2 cut(s) 39, 87
Bse8I GATNNNNATC 1 cut(s) 3115
BseBI CCWGG 6 cut(s) 411, 1545, 2037, 3026, 3135, 3282
BseGI GGATG 7 cut(s) 283, 631, 1094, 1379, 1793, 2119, 2742
BseJI GATNNNNATC 1 cut(s) 3115
BseLI CCNNNNNNNGG 9 cut(s) 471, 683, 1499, 1748, 1777, 2456, 2884, 2969, 3031
BseMI GCAATG 2 cut(s) 39, 87
BseNI ACTGG 9 cut(s) 890, 982, 1625, 2014, 2073, 2539, 2586, 2843, 3160
BseRI GAGGAG 3 cut(s) 32, 1196, 2604
BseXI GCAGC 6 cut(s) 304, 445, 773, 997, 1162, 1942
Bsh1236I CGCG 1 cut(s) 3175
BshFI GGCC 7 cut(s) 1053, 1161, 1577, 1763, 2496, 2779, 3029
BsiSI CCGG 4 cut(s) 592, 1388, 1802, 2825
BslFI GGGAC 4 cut(s) 1119, 2629, 2869, 3003
BslI CCNNNNNNNGG 9 cut(s) 471, 683, 1499, 1748, 1777, 2456, 2884, 2969, 3031
BsmAI GTCTC 4 cut(s) 100, 549, 2213, 2594
BsmFI GGGAC 4 cut(s) 1119, 2629, 2869, 3003
BsnI GGCC 7 cut(s) 1053, 1161, 1577, 1763, 2496, 2779, 3029
Bsp1286I GDGCHC 3 cut(s) 1483, 2716, 2986
Bsp1407I TGTACA 1 cut(s) 164
Bsp19I CCATGG 1 cut(s) 2302
BspACI CCGC 7 cut(s) 23, 674, 1311, 3058, 3173, 3175, 3225
BspANI GGCC 7 cut(s) 1053, 1161, 1577, 1763, 2496, 2779, 3029
BspFNI CGCG 1 cut(s) 3175
BspHI TCATGA 4 cut(s) 610, 1324, 1963, 2592
BspLI GGNNCC 7 cut(s) 190, 687, 1309, 1393, 2041, 2811, 3132
BspMAI CTGCAG 1 cut(s) 2639
BspMI ACCTGC 2 cut(s) 781, 2638
BsrDI GCAATG 2 cut(s) 39, 87
BsrGI TGTACA 1 cut(s) 164
BsrI ACTGG 9 cut(s) 890, 982, 1625, 2014, 2073, 2539, 2586, 2843, 3160
BssT1I CCWWGG 9 cut(s) 1061, 1073, 1591, 1742, 2302, 2423, 2484, 2964, 3030
Bst2UI CCWGG 6 cut(s) 411, 1545, 2037, 3026, 3135, 3282
Bst4CI ACNGT 9 cut(s) 148, 164, 258, 519, 1201, 1300, 2444, 3239, 3289
Bst6I CTCTTC 3 cut(s) 116, 1265, 2905
BstAUI TGTACA 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 1505, 2179, 2635, 3250
BstDSI CCRYGG 2 cut(s) 2302, 3173
BstENI CCTNNNNNAGG 1 cut(s) 3029
BstF5I GGATG 7 cut(s) 283, 631, 1094, 1379, 1793, 2119, 2742
BstFNI CGCG 1 cut(s) 3175
BstHHI GCGC 1 cut(s) 2286
BstMAI GTCTC 4 cut(s) 100, 549, 2213, 2594
BstMWI GCNNNNNNNGC 7 cut(s) 29, 98, 605, 818, 1807, 1927, 2502
BstNI CCWGG 6 cut(s) 411, 1545, 2037, 3026, 3135, 3282
BstNSI RCATGY 3 cut(s) 1507, 1816, 2181
BstSCI CCNGG 7 cut(s) 409, 1387, 1543, 2035, 3024, 3133, 3280
BstSFI CTRYAG 6 cut(s) 550, 990, 1359, 1702, 2604, 2635
BstUI CGCG 1 cut(s) 3175
BstV1I GCAGC 6 cut(s) 304, 445, 773, 997, 1162, 1942
BstV2I GAAGAC 3 cut(s) 92, 980, 2881
BstX2I RGATCY 5 cut(s) 188, 1067, 2021, 2039, 2234
BstYI RGATCY 5 cut(s) 188, 1067, 2021, 2039, 2234
Bsu36I CCTNAGG 1 cut(s) 2322
BsuRI GGCC 7 cut(s) 1053, 1161, 1577, 1763, 2496, 2779, 3029
BtgI CCRYGG 2 cut(s) 2302, 3173
BtsCI GGATG 7 cut(s) 283, 631, 1094, 1379, 1793, 2119, 2742
BtsI GCAGTG 2 cut(s) 2808, 2911
BtsIMutI CAGTG 5 cut(s) 160, 524, 897, 2808, 2911
BveI ACCTGC 2 cut(s) 781, 2638
Cac8I GCNNGC 4 cut(s) 1505, 2179, 2635, 3250
CaiI CAGNNNCTG 2 cut(s) 2255, 2480
CciI TCATGA 4 cut(s) 610, 1324, 1963, 2592
CfoI GCGC 1 cut(s) 2286
Cfr13I GGNCC 7 cut(s) 686, 887, 1159, 1534, 1608, 1761, 2840
Cfr42I CCGCGG 1 cut(s) 3176
CseI GACGC 2 cut(s) 765, 827
CsiI ACCWGGT 2 cut(s) 409, 3280
Csp6I GTAC 8 cut(s) 165, 205, 1768, 1955, 2145, 2445, 3170, 3285
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviQI GTAC 8 cut(s) 165, 205, 1768, 1955, 2145, 2445, 3170, 3285
DraI TTTAAA 1 cut(s) 3115
DrdI GACNNNNNNGTC 2 cut(s) 2227, 2498
DseDI GACNNNNNNGTC 2 cut(s) 2227, 2498
EaeI YGGCCR 2 cut(s) 2494, 3027
Eam1104I CTCTTC 3 cut(s) 116, 1265, 2905
EarI CTCTTC 3 cut(s) 116, 1265, 2905
Eco130I CCWWGG 9 cut(s) 1061, 1073, 1591, 1742, 2302, 2423, 2484, 2964, 3030
Eco24I GRGCYC 3 cut(s) 1483, 2716, 2986
Eco47I GGWCC 5 cut(s) 686, 887, 1534, 1608, 2840
Eco57I CTGAAG 2 cut(s) 200, 2588
Eco81I CCTNAGG 1 cut(s) 2322
EcoNI CCTNNNNNAGG 1 cut(s) 3029
EcoO109I RGGNCCY 2 cut(s) 686, 1534
EcoRI GAATTC 3 cut(s) 210, 943, 2654
EcoRII CCWGG 6 cut(s) 409, 1543, 2035, 3024, 3133, 3280
EcoT14I CCWWGG 9 cut(s) 1061, 1073, 1591, 1742, 2302, 2423, 2484, 2964, 3030
EcoT38I GRGCYC 3 cut(s) 1483, 2716, 2986
ErhI CCWWGG 9 cut(s) 1061, 1073, 1591, 1742, 2302, 2423, 2484, 2964, 3030
FalI AAGNNNNNCTT 2 cut(s) 2206, 2238
FaqI GGGAC 4 cut(s) 1119, 2629, 2869, 3003
FauI CCCGC 2 cut(s) 3051, 3218
FauNDI CATATG 2 cut(s) 1680, 2796
FblI GTMKAC 1 cut(s) 3241
Fnu4HI GCNGC 7 cut(s) 318, 459, 674, 762, 1011, 1176, 1931
FokI GGATG 7 cut(s) 270, 618, 1081, 1366, 1800, 2126, 2749
FriOI GRGCYC 3 cut(s) 1483, 2716, 2986
Fsp4HI GCNGC 7 cut(s) 318, 459, 674, 762, 1011, 1176, 1931
FspBI CTAG 9 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096, 2232, 2535
GlaI GCGC 1 cut(s) 2285
GluI GCNGC 7 cut(s) 318, 459, 674, 762, 1011, 1176, 1931
GsuI CTGGAG 2 cut(s) 1592, 3117
HaeIII GGCC 7 cut(s) 1053, 1161, 1577, 1763, 2496, 2779, 3029
HapII CCGG 4 cut(s) 592, 1388, 1802, 2825
HgaI GACGC 2 cut(s) 765, 827
HhaI GCGC 1 cut(s) 2286
Hin6I GCGC 1 cut(s) 2284
HinP1I GCGC 1 cut(s) 2284
HincII GTYRAC 2 cut(s) 1296, 3242
HindII GTYRAC 2 cut(s) 1296, 3242
HindIII AAGCTT 2 cut(s) 1860, 2204
HpaI GTTAAC 1 cut(s) 1296
HpaII CCGG 4 cut(s) 592, 1388, 1802, 2825
Hpy166II GTNNAC 6 cut(s) 160, 1208, 1296, 2524, 2726, 3242
Hpy8I GTNNAC 6 cut(s) 160, 1208, 1296, 2524, 2726, 3242
Hpy99I CGWCG 1 cut(s) 2495
HpyCH4III ACNGT 9 cut(s) 148, 164, 258, 519, 1201, 1300, 2444, 3239, 3289
HpyCH4IV ACGT 4 cut(s) 370, 696, 1006, 2147
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 98, 605, 818, 1807, 1927, 2502
HpySE526I ACGT 4 cut(s) 370, 696, 1006, 2147
HspAI GCGC 1 cut(s) 2284
KspAI GTTAAC 1 cut(s) 1296
KspI CCGCGG 1 cut(s) 3176
LmnI GCTCC 6 cut(s) 77, 579, 1391, 2459, 3136, 3259
Lsp1109I GCAGC 6 cut(s) 304, 445, 773, 997, 1162, 1942
LweI GCATC 8 cut(s) 45, 578, 640, 748, 1388, 1686, 2383, 2727
MabI ACCWGGT 2 cut(s) 409, 3280
MaeI CTAG 9 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096, 2232, 2535
MaeII ACGT 4 cut(s) 370, 696, 1006, 2147
MaeIII GTNAC 7 cut(s) 138, 241, 486, 1055, 2099, 2170, 3233
MfeI CAATTG 1 cut(s) 1163
MflI RGATCY 5 cut(s) 188, 1067, 2021, 2039, 2234
MhlI GDGCHC 3 cut(s) 1483, 2716, 2986
MlsI TGGCCA 1 cut(s) 3029
MluNI TGGCCA 1 cut(s) 3029
MlyI GAGTC 3 cut(s) 102, 1141, 2255
MmeI TCCRAC 2 cut(s) 336, 3067
Mox20I TGGCCA 1 cut(s) 3029
MscI TGGCCA 1 cut(s) 3029
MseI TTAA 9 cut(s) 324, 387, 1295, 1551, 1668, 2432, 2760, 2918, 3114
MslI CAYNNNNRTG 1 cut(s) 2186
Msp20I TGGCCA 1 cut(s) 3029
MspA1I CMGCKG 2 cut(s) 995, 3175
MspI CCGG 4 cut(s) 592, 1388, 1802, 2825
MspR9I CCNGG 7 cut(s) 411, 1389, 1545, 2037, 3026, 3135, 3282
MunI CAATTG 1 cut(s) 1163
MvaI CCWGG 6 cut(s) 411, 1545, 2037, 3026, 3135, 3282
MvnI CGCG 1 cut(s) 3175
MwoI GCNNNNNNNGC 7 cut(s) 29, 98, 605, 818, 1807, 1927, 2502
NciI CCSGG 1 cut(s) 1389
NcoI CCATGG 1 cut(s) 2302
NdeI CATATG 2 cut(s) 1680, 2796
NlaIV GGNNCC 7 cut(s) 190, 687, 1309, 1393, 2041, 2811, 3132
NmuCI GTSAC 4 cut(s) 241, 486, 1055, 3233
NspI RCATGY 3 cut(s) 1507, 1816, 2181
PaeI GCATGC 2 cut(s) 1507, 2181
PagI TCATGA 4 cut(s) 610, 1324, 1963, 2592
PaqCI CACCTGC 1 cut(s) 2638
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PfeI GAWTC 9 cut(s) 220, 638, 661, 1990, 2084, 2477, 2554, 3138, 3205
PflMI CCANNNNNTGG 1 cut(s) 1499
PfoI TCCNGGA 2 cut(s) 1387, 3133
PkrI GCNGC 7 cut(s) 319, 460, 675, 763, 1012, 1177, 1932
PleI GAGTC 3 cut(s) 102, 1140, 2254
PpsI GAGTC 3 cut(s) 102, 1140, 2254
PpuMI RGGWCCY 2 cut(s) 686, 1534
Psp1406I AACGTT 1 cut(s) 370
Psp5II RGGWCCY 2 cut(s) 686, 1534
Psp6I CCWGG 6 cut(s) 409, 1543, 2035, 3024, 3133, 3280
PspGI CCWGG 6 cut(s) 409, 1543, 2035, 3024, 3133, 3280
PspN4I GGNNCC 7 cut(s) 190, 687, 1309, 1393, 2041, 2811, 3132
PspPI GGNCC 7 cut(s) 686, 887, 1159, 1534, 1608, 1761, 2840
PspPPI RGGWCCY 2 cut(s) 686, 1534
PsrI GAACNNNNNNTAC 2 cut(s) 141, 173
PstI CTGCAG 1 cut(s) 2639
PstNI CAGNNNCTG 2 cut(s) 2255, 2480
PsuI RGATCY 5 cut(s) 188, 1067, 2021, 2039, 2234
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 8 cut(s) 166, 206, 1769, 1956, 2146, 2446, 3171, 3286
RsaNI GTAC 8 cut(s) 165, 205, 1768, 1955, 2145, 2445, 3170, 3285
RseI CAYNNNNRTG 1 cut(s) 2186
SacII CCGCGG 1 cut(s) 3176
SalI GTCGAC 1 cut(s) 3240
SaqAI TTAA 9 cut(s) 324, 387, 1295, 1551, 1668, 2432, 2760, 2918, 3114
SatI GCNGC 7 cut(s) 318, 459, 674, 762, 1011, 1176, 1931
Sau96I GGNCC 7 cut(s) 686, 887, 1159, 1534, 1608, 1761, 2840
SchI GAGTC 3 cut(s) 102, 1141, 2255
ScrFI CCNGG 7 cut(s) 411, 1389, 1545, 2037, 3026, 3135, 3282
SduI GDGCHC 3 cut(s) 1483, 2716, 2986
SexAI ACCWGGT 2 cut(s) 409, 3280
SfaNI GCATC 8 cut(s) 45, 578, 640, 748, 1388, 1686, 2383, 2727
SfcI CTRYAG 6 cut(s) 550, 990, 1359, 1702, 2604, 2635
Sfr303I CCGCGG 1 cut(s) 3176
SgrBI CCGCGG 1 cut(s) 3176
SinI GGWCC 5 cut(s) 686, 887, 1534, 1608, 2840
SmiI ATTTAAAT 1 cut(s) 3115
SmiMI CAYNNNNRTG 1 cut(s) 2186
SmlI CTYRAG 3 cut(s) 802, 1659, 2754
SmoI CTYRAG 3 cut(s) 802, 1659, 2754
SphI GCATGC 2 cut(s) 1507, 2181
SsiI CCGC 7 cut(s) 23, 674, 1311, 3058, 3173, 3175, 3225
SspI AATATT 2 cut(s) 445, 3123
SspMI CTAG 9 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096, 2232, 2535
StyD4I CCNGG 7 cut(s) 409, 1387, 1543, 2035, 3024, 3133, 3280
StyI CCWWGG 9 cut(s) 1061, 1073, 1591, 1742, 2302, 2423, 2484, 2964, 3030
SwaI ATTTAAAT 1 cut(s) 3115
TaaI ACNGT 9 cut(s) 148, 164, 258, 519, 1201, 1300, 2444, 3239, 3289
TaiI ACGT 4 cut(s) 373, 699, 1009, 2150
TaqI TCGA 3 cut(s) 123, 1857, 3241
TatI WGTACW 1 cut(s) 164
TauI GCSGC 1 cut(s) 676
TfiI GAWTC 9 cut(s) 220, 638, 661, 1990, 2084, 2477, 2554, 3138, 3205
Tru1I TTAA 9 cut(s) 324, 387, 1295, 1551, 1668, 2432, 2760, 2918, 3114
Tru9I TTAA 9 cut(s) 324, 387, 1295, 1551, 1668, 2432, 2760, 2918, 3114
TscAI CASTG 5 cut(s) 167, 524, 897, 2808, 2918
TseFI GTSAC 4 cut(s) 241, 486, 1055, 3233
TseI GCWGC 6 cut(s) 317, 458, 761, 1010, 1175, 1930
Tsp45I GTSAC 4 cut(s) 241, 486, 1055, 3233
TspGWI ACGGA 3 cut(s) 222, 566, 2472
TspRI CASTG 5 cut(s) 167, 524, 897, 2808, 2918
Van91I CCANNNNNTGG 1 cut(s) 1499
VpaK11BI GGWCC 5 cut(s) 686, 887, 1534, 1608, 2840
XagI CCTNNNNNAGG 1 cut(s) 3029
XbaI TCTAGA 3 cut(s) 917, 1246, 2231
XceI RCATGY 3 cut(s) 1507, 1816, 2181
XcmI CCANNNNNNNNNTGG 1 cut(s) 507
XmaJI CCTAGG 2 cut(s) 1061, 1742
XmiI GTMKAC 1 cut(s) 3241
XspI CTAG 9 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096, 2232, 2535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.