MD04G1213700.v1.1

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000587
Physical Location & Seq
Reverse (-)
29719330 .. 29724244
4915 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1213700.v1.1.491

Sequence Viewer

Length: 3237 bp
ATGGAGTGCAACAAAGAGGAGGCGTTCAAAGCAATGCAACTATCAGAAGTAAAGATGCGAAACAGTGATTTTACAGGAGCAAGGAAGATGGCACAAAAGGCACAAAAACTCTTTCCGGGAATTGAGAACATTGAGAAATTACTTACAGTCTGTGAAGTTCACTGTTCATCGGAGAACAAAATAGGATTTGAAATGGACTGGTATGGAATTCTTCAGATTCAGAAATCTGATGATGATGTAACCATCAAGAAACAGTACAGGAAGCTGGCGTTGTTACTTCATCCTGATAAGAATAAGTTTGCTGGTGCAGAGGCTGCTTTTAAGTTGATTGGGGAAGCCAACAGGGTGCTAACAGACCAAGCAAAGCGCTCTACGTATGACATGAAGTGTAGAGCTCAAGTGAAAACTGGTTCTTCAATACCATCAGCTCACCCGTCAAATGGAAACTTATTTGTCAGGAAACAAAATGATACTCCTCAGTCACAGTTCCCACCGGACACATTCTGGACATGTTGTCCTTTTTGTAAGATAAAATACCAATACTTCAAAGATTTTGCAAATCGATTGCTGCGCTGTCAAAAATGCAGAAGAGCCTTTGTTGCTCAAGATTTGGGTATTCAATCTCAGGATGCTCATCCAGAATCTGTTGGGAATCAATTTCCAAATCGAAAAGAACCTCCAAATCAGGGTACTTCAAATGCAGCCCCACAAAGTAATGGTGGTACTGGAAAGCCATCCTCCACACAATTTCATTATGGAAAGGCAACCTCAAATCCGCCATCAAAGGCGGGAATTGCCACTGATGTTAAAATATCCCAGTCTGGTCCTGTGAAGTCTCCTGTGAAATCCAAGGATTCAAAAACTTCAGGAAATATGAACAAAAAAAGAGGAAGGGAATCCAGTGAAAGTTGTAAACCTTCTGAACTCAATGAAGTCCATCACCATAGGAGATCATCGAGGATCAAACAGAATCTTTCTTACAATGAAAATCTAAATGATGATGATGATTTTGTGAGTCCGCCCAAAAGGTCGACGGATAGTCAATTTTCCAGTGATACTGAAATGGAGAGGAAGAATGCAGCTGCGGATGGTGGGGTATCTAAAAATAATAGTCAACCAGGTTGTGCTACTACTGCTGCAGTGGGTGGACATAAGAAAGAGGCAAAACAAAAGGTGACTCACCCTCTAGAAGAATGTTTGCCCAGCAAAAGAAGCAAAACTGCTGAATTCGAGCAAAAGGTGGAGGAAGCAGCTATGCCAGATAAAAATGACGATAAGTCCAAAGCTGGTAATGGTTCTGGACCAAATTCAAATGTCACATCCATACCAGCAGGCGTTCTGGTTCCTGATCCTGAATTCAATAAGTTCATGCTTGATGTGGATACGTTAGAGAATGTGTTTAGTGCTAATCAAACGTGGGCTCTTTACGATCCACTAGATGGACTGCCCAGATTCTATGCTCGTATCAAGAAAGTATTCTCCCCCAGATTTAAGCTGAGATTCACCTGGTTGGAGCCCAGTCCAGATGACCAACGTGAGGTTGCTTGGTGTAACAAGGAGTTGCCTGTTGCTTGTGGCAAGTACACGCTTGGTGCCACTGAAGAAGTTACAGATCATCTTATGTTTTCTCATCAGATGCACTGCATAAAAGGCAGTGGCAGAAGCTCTTTTTTCGTATATCCTAAGAAGGGAGAAACCTGGGCCATCTACCAGAATTGGGATATTGGGTGGAGTTCGGAGCCTGAAAAACATGTACCGTACAAGTTTGATTTTGTGGAAGTATTGTCGGATTTTGTCGAAAATTATGGCGTTGGAGTTGCTTATCTCGGCAAAGTAAAAGGTTTTGTCAGTCTATTTCAGCGAAGGGAGCAGCATGGGGTTGTCATGTTTCAAGTACCACCGAATGAGCTGTATAGATTTTCTCATCGAATTCCCTCTTTCAAGATGACAGGTACTGAGAGAGAGGGTGTTCCAGAAGGATCCTTTGAGTTTGATCCTGCTGCTTTGCCCACCAATTTTGATGACCTCATTGCTTCGAAGACGGACAACAGAACCATGAATACTGAAGCTAATGGTTCGTCATGTGACATAAGCAAAAGCAAAACCAAACCTGTAATGAGTTCTGGGAAAGTGTGCGCCGCTGAAAAACAGGAGAAGAACTATTCAGAAAGAGAGACTTCAACACGTAGATCTCCACGAATGTCGAATGGCGAGTTTGCGAATTCTGTTAAGCTAGAAGCAACTCAAGGTATTACTGAAGACAATGGCACAAACCAGGGTAACCAAACTCAACCCAGGGTAAGTTCTACTCCGCGGAAGGCTGATGAGAGTATCAACACACCAAAGAAGCATCAGAAAAATGATTCCGATGGTAAGTCTTTCAGCCTTAGAAGATCACCAAGGGATGTAAGCAAGAACATTCCGAGACCAAATGTATATGTGAAGTGCCCAGATTCCGCCAACGATACAAGCCATGCCAGCTTCACTCCGACTAAAGTCAACTCTACTTCTAGTCAGGCACATTCAAGTGTGAAGGATCATCCTTCTGTCAATTCCATGAAAATCCCCGTCGCCCCTTCATCTTCATCTCCTCTCCATATTTTATCCCGATCTCAGTTCTATGACTTTGATGGGCAAAGGTCCGAGGACAAATTTCGGCTTGATCAAATCTGGGCGCTGTATAGTGATAAGAACGGGATGCCTAGGACTTACGCACAAGTTAAGAGGATTGTGTTGAAGCCAAATTTTCAACTCCATGTAGCGTTGCTCGAACCTTGCTTACCAGAAGATGCGAGTGAACCGGTTTGTTGTGGGACATTTAAAGTGAAGAGTGGTCAAACCAAGGTCATCTCCCGCTCGTCGTTCTCTCATTGTGTGAAAGCCAAACCTATTGGGAAGATGATGTTTGAAATATACCCCAGGAAAGGTGAAGTTTGGGCATTATACAAGAACTCAGGGAAGGGTGAATGCGAAATGGTACAAGTCCTGGAAGATAACGACGAAAGCACAAAGGTTGCGGTTCTGCCGCACGTTAACGGATCCAAGTCGTTGTTTAGGGCCCCAAGAATCCACAGATCGAAGAATGGCATCATCGACATACCAAGGGCTGAGTTAGGTAGATTCTCTCACCAAGCTCCGGCTTTTCTTCATACCGGGGAGATTGACATCCAGCTTGCAAGCTGCTGGGAGGTTGATCAGGTATCAATTCCTGGTACTATTACGATCGTTTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

1079

Amino Acids

120.26

Weight (kDa)

9.0

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 66 - 127 4e-20 DnaJ domain
DUF3444 PF11926 447 - 653 2.5e-77 Domain of unknown function (DUF3444)
DUF3444 PF11926 869 - 1053 1.9e-50 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1592
AccB7I CCANNNNNTGG 1 cut(s) 1439
AccBSI CCGCTC 1 cut(s) 2856
AccI GTMKAC 1 cut(s) 1031
AccII CGCG 1 cut(s) 2314
AclWI GGATC 9 cut(s) 968, 1343, 1424, 1974, 1987, 1988, 2544, 3033, 3046
AcsI RAATTY 8 cut(s) 207, 1226, 1306, 1355, 1929, 2221, 2651, 2743
AcuI CTGAAG 5 cut(s) 197, 849, 1620, 2085, 2277
AfeI AGCGCT 1 cut(s) 368
AfiI CCNNNNNNNGG 8 cut(s) 440, 686, 1439, 1537, 1688, 1717, 2924, 3136
AflIII ACRYGT 3 cut(s) 509, 1750, 2183
AgeI ACCGGT 1 cut(s) 2800
AhdI GACNNNNNGTC 3 cut(s) 513, 1038, 1276
AjnI CCWGG 8 cut(s) 1117, 1505, 1697, 2274, 2294, 2918, 2985, 3208
Alw21I GWGCWC 1 cut(s) 397
Alw26I GTCTC 3 cut(s) 840, 2168, 2419
AlwI GGATC 9 cut(s) 968, 1343, 1424, 1974, 1987, 1988, 2544, 3033, 3046
AlwNI CAGNNNCTG 3 cut(s) 314, 644, 1955
Aor51HI AGCGCT 1 cut(s) 368
AoxI GGCC 2 cut(s) 1701, 3057
ApaI GGGCCC 1 cut(s) 3061
ApoI RAATTY 8 cut(s) 207, 1226, 1306, 1355, 1929, 2221, 2651, 2743
AsiGI ACCGGT 1 cut(s) 2800
Asp700I GAANNNNTTC 1 cut(s) 1475
AspA2I CCTAGG 1 cut(s) 2702
AspLEI GCGC 4 cut(s) 369, 573, 2138, 2677
AspS9I GGNCC 6 cut(s) 824, 1301, 1701, 2640, 3057, 3058
AsuC2I CCSGG 2 cut(s) 117, 3154
AsuHPI GGTGA 9 cut(s) 422, 932, 1172, 1186, 1495, 2388, 2939, 2975, 3119
AsuII TTCGAA 1 cut(s) 2036
AvaII GGWCC 3 cut(s) 824, 1301, 2640
AvrII CCTAGG 1 cut(s) 2702
BaeGI GKGCMC 2 cut(s) 2450, 3061
BamHI GGATCC 2 cut(s) 1979, 3038
BanI GGYRCC 1 cut(s) 1592
BanII GRGCYC 4 cut(s) 397, 1423, 1518, 3061
BbsI GAAGAC 2 cut(s) 2045, 2265
Bbv12I GWGCWC 1 cut(s) 397
BciT130I CCWGG 8 cut(s) 1119, 1507, 1699, 2276, 2296, 2920, 2987, 3210
BciVI GTATCC 1 cut(s) 1375
BclI TGATCA 2 cut(s) 2662, 3193
BcnI CCSGG 2 cut(s) 117, 3154
BcoDI GTCTC 3 cut(s) 840, 2168, 2419
BfaI CTAG 5 cut(s) 1187, 1436, 2234, 2511, 2703
BfmI CTRYAG 1 cut(s) 1137
BfoI RGCGCY 2 cut(s) 370, 2678
BfuI GTATCC 1 cut(s) 1375
BglII AGATCT 1 cut(s) 2189
BlnI CCTAGG 1 cut(s) 2702
Bme18I GGWCC 3 cut(s) 824, 1301, 2640
BmeRI GACNNNNNGTC 3 cut(s) 513, 1038, 1276
BmgT120I GGNCC 6 cut(s) 824, 1301, 1701, 2640, 3057, 3058
BmiI GGNNCC 8 cut(s) 1344, 1515, 1594, 1740, 1981, 3040, 3059, 3060
BmrI ACTGGG 2 cut(s) 811, 1512
BmsI GCATC 7 cut(s) 45, 619, 1626, 2359, 2688, 2779, 3096
BmuI ACTGGG 2 cut(s) 811, 1512
BoxI GACNNNNGTC 1 cut(s) 2495
BpiI GAAGAC 2 cut(s) 2045, 2265
Bpu14I TTCGAA 1 cut(s) 2036
BpuEI CTTGAG 3 cut(s) 381, 588, 2229
BpuMI CCSGG 2 cut(s) 117, 3154
Bsa29I ATCGAT 1 cut(s) 562
BsaAI YACGTR 2 cut(s) 375, 2186
BsaBI GATNNNNATC 2 cut(s) 633, 3164
BsaI GGTCTC 1 cut(s) 2419
BsaWI WCCGGW 2 cut(s) 493, 2800
Bsc4I CCNNNNNNNGG 8 cut(s) 440, 686, 1439, 1537, 1688, 1717, 2924, 3136
Bse118I RCCGGY 1 cut(s) 2800
Bse1I ACTGG 7 cut(s) 203, 412, 730, 817, 900, 1050, 1518
Bse3DI GCAATG 2 cut(s) 39, 2028
Bse8I GATNNNNATC 2 cut(s) 633, 3164
BseBI CCWGG 8 cut(s) 1119, 1507, 1699, 2276, 2296, 2920, 2987, 3210
BseCI ATCGAT 1 cut(s) 562
BseJI GATNNNNATC 2 cut(s) 633, 3164
BseLI CCNNNNNNNGG 8 cut(s) 440, 686, 1439, 1537, 1688, 1717, 2924, 3136
BseMI GCAATG 2 cut(s) 39, 2028
BseMII CTCAG 7 cut(s) 491, 638, 1487, 1947, 2627, 2967, 3099
BseNI ACTGG 7 cut(s) 203, 412, 730, 817, 900, 1050, 1518
BseRI GAGGAG 3 cut(s) 32, 465, 2580
BseSI GKGCMC 2 cut(s) 2450, 3061
BseYI CCCAGC 2 cut(s) 1202, 3183
BsgI GTGCAG 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 2314
Bsh1285I CGRYCG 1 cut(s) 3225
BshFI GGCC 2 cut(s) 1703, 3059
BshNI GGYRCC 1 cut(s) 1592
BshTI ACCGGT 1 cut(s) 2800
BshVI ATCGAT 1 cut(s) 562
BsiEI CGRYCG 1 cut(s) 3225
BsiHKAI GWGCWC 1 cut(s) 397
BsiSI CCGG 5 cut(s) 116, 494, 2801, 3137, 3153
BslFI GGGAC 1 cut(s) 2827
BslI CCNNNNNNNGG 8 cut(s) 440, 686, 1439, 1537, 1688, 1717, 2924, 3136
BsmAI GTCTC 3 cut(s) 840, 2168, 2419
BsmFI GGGAC 1 cut(s) 2827
BsmI GAATGC 2 cut(s) 1081, 2972
BsnI GGCC 2 cut(s) 1703, 3059
Bso31I GGTCTC 1 cut(s) 2419
Bsp119I TTCGAA 1 cut(s) 2036
Bsp120I GGGCCC 1 cut(s) 3057
Bsp1286I GDGCHC 5 cut(s) 397, 1423, 1518, 2450, 3061
BspANI GGCC 2 cut(s) 1703, 3059
BspCNI CTCAG 7 cut(s) 490, 637, 1488, 1948, 2626, 2966, 3100
BspDI ATCGAT 1 cut(s) 562
BspFNI CGCG 1 cut(s) 2314
BspLI GGNNCC 8 cut(s) 1344, 1515, 1594, 1740, 1981, 3040, 3059, 3060
BspMAI CTGCAG 1 cut(s) 1141
BspPI GGATC 9 cut(s) 968, 1343, 1424, 1974, 1987, 1988, 2544, 3033, 3046
BspQI GCTCTTC 1 cut(s) 583
BspT104I TTCGAA 1 cut(s) 2036
BspT107I GGYRCC 1 cut(s) 1592
BspTNI GGTCTC 1 cut(s) 2419
BsrBI CCGCTC 1 cut(s) 2856
BsrDI GCAATG 2 cut(s) 39, 2028
BsrFI RCCGGY 1 cut(s) 2800
BsrI ACTGG 7 cut(s) 203, 412, 730, 817, 900, 1050, 1518
BssAI RCCGGY 1 cut(s) 2800
BssT1I CCWWGG 5 cut(s) 849, 2399, 2702, 2841, 3101
Bst2UI CCWGG 8 cut(s) 1119, 1507, 1699, 2276, 2296, 2920, 2987, 3210
Bst4CI ACNGT 6 cut(s) 65, 148, 164, 255, 486, 1758
Bst6I CTCTTC 2 cut(s) 583, 2822
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBAI YACGTR 2 cut(s) 375, 2186
BstBI TTCGAA 1 cut(s) 2036
BstC8I GCNNGC 5 cut(s) 267, 1333, 2479, 3174, 3178
BstDEI CTNAG 9 cut(s) 477, 624, 1496, 1683, 1956, 2387, 2613, 2953, 3108
BstDSI CCRYGG 1 cut(s) 2312
BstEII GGTNACC 1 cut(s) 2279
BstFNI CGCG 1 cut(s) 2314
BstH2I RGCGCY 2 cut(s) 370, 2678
BstHHI GCGC 4 cut(s) 369, 573, 2138, 2677
BstMAI GTCTC 3 cut(s) 840, 2168, 2419
BstMCI CGRYCG 1 cut(s) 3225
BstNI CCWGG 8 cut(s) 1119, 1507, 1699, 2276, 2296, 2920, 2987, 3210
BstNSI RCATGY 2 cut(s) 513, 1754
BstPAI GACNNNNGTC 1 cut(s) 2495
BstPI GGTNACC 1 cut(s) 2279
BstSFI CTRYAG 1 cut(s) 1137
BstSLI GKGCMC 2 cut(s) 2450, 3061
BstSNI TACGTA 1 cut(s) 375
BstUI CGCG 1 cut(s) 2314
BstV2I GAAGAC 2 cut(s) 2045, 2265
BstX2I RGATCY 3 cut(s) 1979, 2189, 3038
BstYI RGATCY 3 cut(s) 1979, 2189, 3038
Bsu15I ATCGAT 1 cut(s) 562
BsuI GTATCC 1 cut(s) 1375
BsuRI GGCC 2 cut(s) 1703, 3059
BsuTUI ATCGAT 1 cut(s) 562
BtgI CCRYGG 1 cut(s) 2312
BtsI GCAGTG 3 cut(s) 1146, 1639, 1660
BtsIMutI CAGTG 9 cut(s) 70, 160, 798, 907, 1057, 1146, 1596, 1639, 1660
Cac8I GCNNGC 5 cut(s) 267, 1333, 2479, 3174, 3178
CaiI CAGNNNCTG 3 cut(s) 314, 644, 1955
CfoI GCGC 4 cut(s) 369, 573, 2138, 2677
Cfr10I RCCGGY 1 cut(s) 2800
Cfr13I GGNCC 6 cut(s) 824, 1301, 1701, 2640, 3057, 3058
Cfr42I CCGCGG 1 cut(s) 2315
ClaI ATCGAT 1 cut(s) 562
CsiI ACCWGGT 2 cut(s) 1117, 1505
CspAI ACCGGT 1 cut(s) 2800
CspCI CAANNNNNGTGG 2 cut(s) 1999, 2034
DdeI CTNAG 9 cut(s) 477, 624, 1496, 1683, 1956, 2387, 2613, 2953, 3108
DraI TTTAAA 1 cut(s) 2821
DriI GACNNNNNGTC 3 cut(s) 513, 1038, 1276
Eam1104I CTCTTC 2 cut(s) 583, 2822
Eam1105I GACNNNNNGTC 3 cut(s) 513, 1038, 1276
EarI CTCTTC 2 cut(s) 583, 2822
EciI GGCGGA 3 cut(s) 765, 1008, 2446
Ecl136II GAGCTC 1 cut(s) 395
Eco105I TACGTA 1 cut(s) 375
Eco130I CCWWGG 5 cut(s) 849, 2399, 2702, 2841, 3101
Eco24I GRGCYC 4 cut(s) 397, 1423, 1518, 3061
Eco31I GGTCTC 1 cut(s) 2419
Eco47I GGWCC 3 cut(s) 824, 1301, 2640
Eco47III AGCGCT 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 395
Eco57I CTGAAG 5 cut(s) 197, 849, 1620, 2085, 2277
Eco91I GGTNACC 1 cut(s) 2279
EcoICRI GAGCTC 1 cut(s) 395
EcoO109I RGGNCCY 2 cut(s) 3057, 3058
EcoO65I GGTNACC 1 cut(s) 2279
EcoRI GAATTC 5 cut(s) 207, 1226, 1355, 1929, 2221
EcoRII CCWGG 8 cut(s) 1117, 1505, 1697, 2274, 2294, 2918, 2985, 3208
EcoT14I CCWWGG 5 cut(s) 849, 2399, 2702, 2841, 3101
EcoT38I GRGCYC 4 cut(s) 397, 1423, 1518, 3061
ErhI CCWWGG 5 cut(s) 849, 2399, 2702, 2841, 3101
FalI AAGNNNNNCTT 2 cut(s) 2161, 2193
FaqI GGGAC 1 cut(s) 2827
FauI CCCGC 2 cut(s) 781, 2861
FbaI TGATCA 2 cut(s) 2662, 3193
FblI GTMKAC 1 cut(s) 1031
FriOI GRGCYC 4 cut(s) 397, 1423, 1518, 3061
FspBI CTAG 5 cut(s) 1187, 1436, 2234, 2511, 2703
GlaI GCGC 4 cut(s) 368, 572, 2137, 2676
GsaI CCCAGC 2 cut(s) 1206, 3187
HaeII RGCGCY 2 cut(s) 370, 2678
HaeIII GGCC 2 cut(s) 1703, 3059
HapII CCGG 5 cut(s) 116, 494, 2801, 3137, 3153
HhaI GCGC 4 cut(s) 369, 573, 2138, 2677
Hin6I GCGC 4 cut(s) 367, 571, 2136, 2675
HinP1I GCGC 4 cut(s) 367, 571, 2136, 2675
HincII GTYRAC 4 cut(s) 1032, 1115, 2500, 3034
HindII GTYRAC 4 cut(s) 1032, 1115, 2500, 3034
HpaI GTTAAC 1 cut(s) 3034
HpaII CCGG 5 cut(s) 116, 494, 2801, 3137, 3153
HphI GGTGA 9 cut(s) 422, 932, 1172, 1186, 1495, 2388, 2939, 2975, 3119
Hpy166II GTNNAC 9 cut(s) 160, 914, 1032, 1115, 1148, 1584, 2500, 2798, 3034
Hpy8I GTNNAC 9 cut(s) 160, 914, 1032, 1115, 1148, 1584, 2500, 2798, 3034
Hpy99I CGWCG 4 cut(s) 1036, 2573, 2863, 3002
HpyCH4III ACNGT 6 cut(s) 65, 148, 164, 255, 486, 1758
HpyCH4IV ACGT 6 cut(s) 374, 1385, 1415, 1534, 2185, 3030
HpyF3I CTNAG 9 cut(s) 477, 624, 1496, 1683, 1956, 2387, 2613, 2953, 3108
HpySE526I ACGT 6 cut(s) 374, 1385, 1415, 1534, 2185, 3030
HspAI GCGC 4 cut(s) 367, 571, 2136, 2675
Ksp22I TGATCA 2 cut(s) 2662, 3193
KspAI GTTAAC 1 cut(s) 3034
KspI CCGCGG 1 cut(s) 2315
LguI GCTCTTC 1 cut(s) 583
LmnI GCTCC 5 cut(s) 77, 1513, 1738, 1867, 3139
LweI GCATC 7 cut(s) 45, 619, 1626, 2359, 2688, 2779, 3096
MabI ACCWGGT 2 cut(s) 1117, 1505
MaeI CTAG 5 cut(s) 1187, 1436, 2234, 2511, 2703
MaeII ACGT 6 cut(s) 374, 1385, 1415, 1534, 2185, 3030
MaeIII GTNAC 9 cut(s) 238, 273, 480, 1174, 1315, 1550, 1606, 2084, 2279
MbiI CCGCTC 1 cut(s) 2856
MflI RGATCY 3 cut(s) 1979, 2189, 3038
MhlI GDGCHC 5 cut(s) 397, 1423, 1518, 2450, 3061
MlyI GAGTC 2 cut(s) 1024, 1171
MmeI TCCRAC 4 cut(s) 1491, 1767, 1792, 2513
MroXI GAANNNNTTC 1 cut(s) 1475
MseI TTAA 7 cut(s) 321, 807, 1491, 2229, 2721, 2820, 3033
MslI CAYNNNNRTG 1 cut(s) 2526
MspA1I CMGCKG 3 cut(s) 1082, 2141, 2314
MspI CCGG 5 cut(s) 116, 494, 2801, 3137, 3153
Mva1269I GAATGC 2 cut(s) 1081, 2972
MvaI CCWGG 8 cut(s) 1119, 1507, 1699, 2276, 2296, 2920, 2987, 3210
MvnI CGCG 1 cut(s) 2314
NciI CCSGG 2 cut(s) 117, 3154
NlaIV GGNNCC 8 cut(s) 1344, 1515, 1594, 1740, 1981, 3040, 3059, 3060
NmeAIII GCCGAG 1 cut(s) 1806
NmuCI GTSAC 4 cut(s) 480, 1174, 1315, 2084
NspI RCATGY 2 cut(s) 513, 1754
NspV TTCGAA 1 cut(s) 2036
PasI CCCWGGG 1 cut(s) 2295
PciI ACATGT 2 cut(s) 509, 1750
PciSI GCTCTTC 1 cut(s) 583
PctI GAATGC 2 cut(s) 1081, 2972
PdmI GAANNNNTTC 1 cut(s) 1475
PflMI CCANNNNNTGG 1 cut(s) 1439
PfoI TCCNGGA 2 cut(s) 115, 2985
PinAI ACCGGT 1 cut(s) 2800
Ple19I CGATCG 1 cut(s) 3225
PleI GAGTC 2 cut(s) 1023, 1171
PpsI GAGTC 2 cut(s) 1023, 1171
Ppu21I YACGTR 2 cut(s) 375, 2186
PscI ACATGT 2 cut(s) 509, 1750
PshAI GACNNNNGTC 1 cut(s) 2495
Psp124BI GAGCTC 1 cut(s) 397
Psp6I CCWGG 8 cut(s) 1117, 1505, 1697, 2274, 2294, 2918, 2985, 3208
PspEI GGTNACC 1 cut(s) 2279
PspFI CCCAGC 2 cut(s) 1202, 3183
PspGI CCWGG 8 cut(s) 1117, 1505, 1697, 2274, 2294, 2918, 2985, 3208
PspN4I GGNNCC 8 cut(s) 1344, 1515, 1594, 1740, 1981, 3040, 3059, 3060
PspOMI GGGCCC 1 cut(s) 3057
PspPI GGNCC 6 cut(s) 824, 1301, 1701, 2640, 3057, 3058
PsrI GAACNNNNNNTAC 4 cut(s) 2044, 2076, 2763, 2795
PstI CTGCAG 1 cut(s) 1141
PstNI CAGNNNCTG 3 cut(s) 314, 644, 1955
PsuI RGATCY 3 cut(s) 1979, 2189, 3038
PvuI CGATCG 1 cut(s) 3225
PvuII CAGCTG 1 cut(s) 1082
RseI CAYNNNNRTG 1 cut(s) 2526
SacI GAGCTC 1 cut(s) 397
SacII CCGCGG 1 cut(s) 2315
SalI GTCGAC 1 cut(s) 1030
SapI GCTCTTC 1 cut(s) 583
SaqAI TTAA 7 cut(s) 321, 807, 1491, 2229, 2721, 2820, 3033
Sau96I GGNCC 6 cut(s) 824, 1301, 1701, 2640, 3057, 3058
SchI GAGTC 2 cut(s) 1024, 1171
SduI GDGCHC 5 cut(s) 397, 1423, 1518, 2450, 3061
SexAI ACCWGGT 2 cut(s) 1117, 1505
SfaNI GCATC 7 cut(s) 45, 619, 1626, 2359, 2688, 2779, 3096
SfcI CTRYAG 1 cut(s) 1137
Sfr303I CCGCGG 1 cut(s) 2315
SfuI TTCGAA 1 cut(s) 2036
SgrBI CCGCGG 1 cut(s) 2315
SinI GGWCC 3 cut(s) 824, 1301, 2640
SmiMI CAYNNNNRTG 1 cut(s) 2526
SmlI CTYRAG 3 cut(s) 396, 603, 2244
SmoI CTYRAG 3 cut(s) 396, 603, 2244
SnaBI TACGTA 1 cut(s) 375
SspMI CTAG 5 cut(s) 1187, 1436, 2234, 2511, 2703
SstI GAGCTC 1 cut(s) 397
StyI CCWWGG 5 cut(s) 849, 2399, 2702, 2841, 3101
TaaI ACNGT 6 cut(s) 65, 148, 164, 255, 486, 1758
TaiI ACGT 6 cut(s) 377, 1388, 1418, 1537, 2188, 3033
TatI WGTACW 2 cut(s) 255, 1581
TauI GCSGC 2 cut(s) 2141, 3028
Tru1I TTAA 7 cut(s) 321, 807, 1491, 2229, 2721, 2820, 3033
Tru9I TTAA 7 cut(s) 321, 807, 1491, 2229, 2721, 2820, 3033
TscAI CASTG 9 cut(s) 70, 167, 805, 907, 1057, 1146, 1603, 1646, 1660
TseFI GTSAC 4 cut(s) 480, 1174, 1315, 2084
Tsp45I GTSAC 4 cut(s) 480, 1174, 1315, 2084
TspGWI ACGGA 3 cut(s) 1049, 2057, 3051
TspRI CASTG 9 cut(s) 70, 167, 805, 907, 1057, 1146, 1603, 1646, 1660
Van91I CCANNNNNTGG 1 cut(s) 1439
VpaK11BI GGWCC 3 cut(s) 824, 1301, 2640
XapI RAATTY 8 cut(s) 207, 1226, 1306, 1355, 1929, 2221, 2651, 2743
XbaI TCTAGA 1 cut(s) 1186
XceI RCATGY 2 cut(s) 513, 1754
XmaJI CCTAGG 1 cut(s) 2702
XmiI GTMKAC 1 cut(s) 1031
XmnI GAANNNNTTC 1 cut(s) 1475
XspI CTAG 5 cut(s) 1187, 1436, 2234, 2511, 2703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.