Domain of unknown function (DUF3444)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2641 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 2227, 2501 |
| Acc36I | ACCTGC | 2 cut(s) | 781, 2641 |
| AccI | GTMKAC | 1 cut(s) | 3244 |
| AccII | CGCG | 1 cut(s) | 3178 |
| AciI | CCGC | 8 cut(s) | 23, 458, 674, 1311, 3061, 3176, 3178, 3228 |
| AclI | AACGTT | 1 cut(s) | 370 |
| AcoI | YGGCCR | 1 cut(s) | 3030 |
| AcuI | CTGAAG | 2 cut(s) | 200, 2591 |
| AfaI | GTAC | 7 cut(s) | 166, 1769, 1956, 2146, 2449, 3174, 3289 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 471, 683, 1748, 1777, 2459, 2887, 2972, 3034 |
| AjnI | CCWGG | 5 cut(s) | 409, 1543, 2035, 3027, 3283 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1860, 1892, 2013, 2045 |
| Alw26I | GTCTC | 4 cut(s) | 100, 549, 2213, 2597 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2483 |
| Ama87I | CYCGRG | 1 cut(s) | 2321 |
| AoxI | GGCC | 6 cut(s) | 1051, 1159, 1575, 1761, 2780, 3030 |
| ApeKI | GCWGC | 5 cut(s) | 317, 761, 1010, 1175, 1930 |
| AspA2I | CCTAGG | 2 cut(s) | 1061, 1742 |
| AspLEI | GCGC | 1 cut(s) | 2286 |
| AspS9I | GGNCC | 9 cut(s) | 416, 686, 887, 1159, 1208, 1534, 1608, 1761, 2843 |
| AsuC2I | CCSGG | 1 cut(s) | 1389 |
| AvaI | CYCGRG | 1 cut(s) | 2321 |
| AvaII | GGWCC | 7 cut(s) | 416, 686, 887, 1208, 1534, 1608, 2843 |
| AvrII | CCTAGG | 2 cut(s) | 1061, 1742 |
| BalI | TGGCCA | 1 cut(s) | 3032 |
| BamHI | GGATCC | 2 cut(s) | 188, 2039 |
| BanII | GRGCYC | 3 cut(s) | 1483, 2719, 2989 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1431, 1463 |
| BbsI | GAAGAC | 2 cut(s) | 980, 2884 |
| BbvCI | CCTCAGC | 1 cut(s) | 1179 |
| BbvI | GCAGC | 5 cut(s) | 304, 773, 997, 1162, 1942 |
| BccI | CCATC | 7 cut(s) | 82, 1560, 1782, 2135, 2461, 2629, 3280 |
| BceAI | ACGGC | 1 cut(s) | 1038 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2312, 2346 |
| BciT130I | CCWGG | 5 cut(s) | 411, 1545, 2037, 3029, 3285 |
| BciVI | GTATCC | 1 cut(s) | 2245 |
| BcnI | CCSGG | 1 cut(s) | 1389 |
| BcoDI | GTCTC | 4 cut(s) | 100, 549, 2213, 2597 |
| BfmI | CTRYAG | 7 cut(s) | 550, 990, 1359, 1702, 1819, 2607, 2638 |
| BfuAI | ACCTGC | 2 cut(s) | 781, 2641 |
| BfuI | GTATCC | 1 cut(s) | 2245 |
| BglII | AGATCT | 2 cut(s) | 1067, 2234 |
| BisI | GCNGC | 8 cut(s) | 318, 459, 674, 762, 1011, 1176, 1311, 1931 |
| BlnI | CCTAGG | 2 cut(s) | 1061, 1742 |
| BlsI | GCNGC | 8 cut(s) | 319, 460, 675, 763, 1012, 1177, 1312, 1932 |
| Bme1390I | CCNGG | 6 cut(s) | 411, 1389, 1545, 2037, 3029, 3285 |
| Bme18I | GGWCC | 7 cut(s) | 416, 686, 887, 1208, 1534, 1608, 2843 |
| BmeT110I | CYCGRG | 1 cut(s) | 2321 |
| BmgT120I | GGNCC | 9 cut(s) | 416, 686, 887, 1159, 1208, 1534, 1608, 1761, 2843 |
| BmiI | GGNNCC | 7 cut(s) | 190, 687, 1309, 1393, 2041, 2814, 3135 |
| BmrFI | CCNGG | 6 cut(s) | 411, 1389, 1545, 2037, 3029, 3285 |
| BmrI | ACTGGG | 2 cut(s) | 2583, 3168 |
| BmsI | GCATC | 9 cut(s) | 45, 578, 640, 748, 1388, 1686, 2386, 2547, 2730 |
| BmuI | ACTGGG | 2 cut(s) | 2583, 3168 |
| BpiI | GAAGAC | 2 cut(s) | 980, 2884 |
| BpmI | CTGGAG | 1 cut(s) | 1592 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1179, 3009 |
| BpuEI | CTTGAG | 3 cut(s) | 823, 1644, 2742 |
| BpuMI | CCSGG | 1 cut(s) | 1389 |
| BsaBI | GATNNNNATC | 1 cut(s) | 3118 |
| BsaWI | WCCGGW | 1 cut(s) | 2827 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 3084, 3114 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 471, 683, 1748, 1777, 2459, 2887, 2972, 3034 |
| Bse1I | ACTGG | 9 cut(s) | 890, 982, 1625, 2014, 2073, 2542, 2589, 2846, 3163 |
| Bse3DI | GCAATG | 2 cut(s) | 39, 87 |
| Bse8I | GATNNNNATC | 1 cut(s) | 3118 |
| BseBI | CCWGG | 5 cut(s) | 411, 1545, 2037, 3029, 3285 |
| BseJI | GATNNNNATC | 1 cut(s) | 3118 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 471, 683, 1748, 1777, 2459, 2887, 2972, 3034 |
| BseMI | GCAATG | 2 cut(s) | 39, 87 |
| BseNI | ACTGG | 9 cut(s) | 890, 982, 1625, 2014, 2073, 2542, 2589, 2846, 3163 |
| BseRI | GAGGAG | 3 cut(s) | 32, 1196, 2607 |
| BseXI | GCAGC | 5 cut(s) | 304, 773, 997, 1162, 1942 |
| Bsh1236I | CGCG | 1 cut(s) | 3178 |
| BshFI | GGCC | 6 cut(s) | 1053, 1161, 1577, 1763, 2782, 3032 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2321 |
| BsiSI | CCGG | 4 cut(s) | 592, 1388, 1802, 2828 |
| BslFI | GGGAC | 3 cut(s) | 1119, 2872, 3006 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 471, 683, 1748, 1777, 2459, 2887, 2972, 3034 |
| BsmAI | GTCTC | 4 cut(s) | 100, 549, 2213, 2597 |
| BsmFI | GGGAC | 3 cut(s) | 1119, 2872, 3006 |
| BsmI | GAATGC | 1 cut(s) | 2560 |
| BsnI | GGCC | 6 cut(s) | 1053, 1161, 1577, 1763, 2782, 3032 |
| BsoBI | CYCGRG | 1 cut(s) | 2321 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1483, 2719, 2989 |
| Bsp1407I | TGTACA | 1 cut(s) | 164 |
| Bsp19I | CCATGG | 1 cut(s) | 2302 |
| BspACI | CCGC | 8 cut(s) | 23, 458, 674, 1311, 3061, 3176, 3178, 3228 |
| BspANI | GGCC | 6 cut(s) | 1053, 1161, 1577, 1763, 2782, 3032 |
| BspFNI | CGCG | 1 cut(s) | 3178 |
| BspHI | TCATGA | 4 cut(s) | 610, 1324, 1963, 2595 |
| BspLI | GGNNCC | 7 cut(s) | 190, 687, 1309, 1393, 2041, 2814, 3135 |
| BspMAI | CTGCAG | 1 cut(s) | 2642 |
| BspMI | ACCTGC | 2 cut(s) | 781, 2641 |
| BsrDI | GCAATG | 2 cut(s) | 39, 87 |
| BsrGI | TGTACA | 1 cut(s) | 164 |
| BsrI | ACTGG | 9 cut(s) | 890, 982, 1625, 2014, 2073, 2542, 2589, 2846, 3163 |
| BssT1I | CCWWGG | 9 cut(s) | 1061, 1073, 1591, 1742, 2302, 2426, 2487, 2967, 3033 |
| Bst2UI | CCWGG | 5 cut(s) | 411, 1545, 2037, 3029, 3285 |
| Bst4CI | ACNGT | 9 cut(s) | 148, 164, 247, 258, 519, 1201, 1300, 2447, 3242 |
| Bst6I | CTCTTC | 3 cut(s) | 116, 1265, 2908 |
| BstAUI | TGTACA | 1 cut(s) | 164 |
| BstC8I | GCNNGC | 3 cut(s) | 2179, 2638, 3253 |
| BstDSI | CCRYGG | 2 cut(s) | 2302, 3176 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3032 |
| BstFNI | CGCG | 1 cut(s) | 3178 |
| BstHHI | GCGC | 1 cut(s) | 2286 |
| BstMAI | GTCTC | 4 cut(s) | 100, 549, 2213, 2597 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 29, 98, 605, 1927, 2505 |
| BstNI | CCWGG | 5 cut(s) | 411, 1545, 2037, 3029, 3285 |
| BstNSI | RCATGY | 4 cut(s) | 1010, 1507, 1816, 2181 |
| BstSCI | CCNGG | 6 cut(s) | 409, 1387, 1543, 2035, 3027, 3283 |
| BstSFI | CTRYAG | 7 cut(s) | 550, 990, 1359, 1702, 1819, 2607, 2638 |
| BstUI | CGCG | 1 cut(s) | 3178 |
| BstV1I | GCAGC | 5 cut(s) | 304, 773, 997, 1162, 1942 |
| BstV2I | GAAGAC | 2 cut(s) | 980, 2884 |
| BstX2I | RGATCY | 6 cut(s) | 188, 1067, 2021, 2039, 2092, 2234 |
| BstYI | RGATCY | 6 cut(s) | 188, 1067, 2021, 2039, 2092, 2234 |
| BsuI | GTATCC | 1 cut(s) | 2245 |
| BsuRI | GGCC | 6 cut(s) | 1053, 1161, 1577, 1763, 2782, 3032 |
| BtgI | CCRYGG | 2 cut(s) | 2302, 3176 |
| BtsI | GCAGTG | 2 cut(s) | 2811, 2914 |
| BtsIMutI | CAGTG | 5 cut(s) | 160, 524, 897, 2811, 2914 |
| BveI | ACCTGC | 2 cut(s) | 781, 2641 |
| Cac8I | GCNNGC | 3 cut(s) | 2179, 2638, 3253 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2483 |
| CciI | TCATGA | 4 cut(s) | 610, 1324, 1963, 2595 |
| CfoI | GCGC | 1 cut(s) | 2286 |
| Cfr13I | GGNCC | 9 cut(s) | 416, 686, 887, 1159, 1208, 1534, 1608, 1761, 2843 |
| Cfr42I | CCGCGG | 1 cut(s) | 3179 |
| CseI | GACGC | 2 cut(s) | 765, 827 |
| CsiI | ACCWGGT | 2 cut(s) | 409, 3283 |
| Csp6I | GTAC | 7 cut(s) | 165, 1768, 1955, 2145, 2448, 3173, 3288 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 166, 201 |
| CviQI | GTAC | 7 cut(s) | 165, 1768, 1955, 2145, 2448, 3173, 3288 |
| DraI | TTTAAA | 1 cut(s) | 3118 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 2227, 2501 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 2227, 2501 |
| EaeI | YGGCCR | 1 cut(s) | 3030 |
| Eam1104I | CTCTTC | 3 cut(s) | 116, 1265, 2908 |
| EarI | CTCTTC | 3 cut(s) | 116, 1265, 2908 |
| Eco130I | CCWWGG | 9 cut(s) | 1061, 1073, 1591, 1742, 2302, 2426, 2487, 2967, 3033 |
| Eco24I | GRGCYC | 3 cut(s) | 1483, 2719, 2989 |
| Eco47I | GGWCC | 7 cut(s) | 416, 686, 887, 1208, 1534, 1608, 2843 |
| Eco57I | CTGAAG | 2 cut(s) | 200, 2591 |
| Eco88I | CYCGRG | 1 cut(s) | 2321 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3032 |
| EcoO109I | RGGNCCY | 2 cut(s) | 686, 1534 |
| EcoRI | GAATTC | 3 cut(s) | 210, 943, 2657 |
| EcoRII | CCWGG | 5 cut(s) | 409, 1543, 2035, 3027, 3283 |
| EcoT14I | CCWWGG | 9 cut(s) | 1061, 1073, 1591, 1742, 2302, 2426, 2487, 2967, 3033 |
| EcoT22I | ATGCAT | 1 cut(s) | 2562 |
| EcoT38I | GRGCYC | 3 cut(s) | 1483, 2719, 2989 |
| ErhI | CCWWGG | 9 cut(s) | 1061, 1073, 1591, 1742, 2302, 2426, 2487, 2967, 3033 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1662, 1694, 2206, 2238 |
| FaqI | GGGAC | 3 cut(s) | 1119, 2872, 3006 |
| FauI | CCCGC | 2 cut(s) | 3054, 3221 |
| FauNDI | CATATG | 1 cut(s) | 2799 |
| FblI | GTMKAC | 1 cut(s) | 3244 |
| Fnu4HI | GCNGC | 8 cut(s) | 318, 459, 674, 762, 1011, 1176, 1311, 1931 |
| FriOI | GRGCYC | 3 cut(s) | 1483, 2719, 2989 |
| Fsp4HI | GCNGC | 8 cut(s) | 318, 459, 674, 762, 1011, 1176, 1311, 1931 |
| GlaI | GCGC | 1 cut(s) | 2285 |
| GluI | GCNGC | 8 cut(s) | 318, 459, 674, 762, 1011, 1176, 1311, 1931 |
| GsuI | CTGGAG | 1 cut(s) | 1592 |
| HaeIII | GGCC | 6 cut(s) | 1053, 1161, 1577, 1763, 2782, 3032 |
| HapII | CCGG | 4 cut(s) | 592, 1388, 1802, 2828 |
| HgaI | GACGC | 2 cut(s) | 765, 827 |
| HhaI | GCGC | 1 cut(s) | 2286 |
| Hin6I | GCGC | 1 cut(s) | 2284 |
| HinP1I | GCGC | 1 cut(s) | 2284 |
| HincII | GTYRAC | 1 cut(s) | 3245 |
| HindII | GTYRAC | 1 cut(s) | 3245 |
| HindIII | AAGCTT | 2 cut(s) | 1860, 2204 |
| HpaII | CCGG | 4 cut(s) | 592, 1388, 1802, 2828 |
| Hpy166II | GTNNAC | 6 cut(s) | 160, 416, 1208, 1448, 2527, 3245 |
| Hpy8I | GTNNAC | 6 cut(s) | 160, 416, 1208, 1448, 2527, 3245 |
| HpyCH4III | ACNGT | 9 cut(s) | 148, 164, 247, 258, 519, 1201, 1300, 2447, 3242 |
| HpyCH4IV | ACGT | 3 cut(s) | 370, 696, 2147 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 29, 98, 605, 1927, 2505 |
| HpySE526I | ACGT | 3 cut(s) | 370, 696, 2147 |
| HspAI | GCGC | 1 cut(s) | 2284 |
| KspI | CCGCGG | 1 cut(s) | 3179 |
| LmnI | GCTCC | 7 cut(s) | 77, 579, 1307, 1391, 2462, 3139, 3262 |
| Lsp1109I | GCAGC | 5 cut(s) | 304, 773, 997, 1162, 1942 |
| LweI | GCATC | 9 cut(s) | 45, 578, 640, 748, 1388, 1686, 2386, 2547, 2730 |
| MabI | ACCWGGT | 2 cut(s) | 409, 3283 |
| MaeII | ACGT | 3 cut(s) | 370, 696, 2147 |
| MfeI | CAATTG | 1 cut(s) | 1163 |
| MflI | RGATCY | 6 cut(s) | 188, 1067, 2021, 2039, 2092, 2234 |
| MhlI | GDGCHC | 3 cut(s) | 1483, 2719, 2989 |
| MlsI | TGGCCA | 1 cut(s) | 3032 |
| MluNI | TGGCCA | 1 cut(s) | 3032 |
| MlyI | GAGTC | 3 cut(s) | 102, 1141, 2255 |
| MmeI | TCCRAC | 3 cut(s) | 336, 2229, 3070 |
| Mox20I | TGGCCA | 1 cut(s) | 3032 |
| Mph1103I | ATGCAT | 1 cut(s) | 2562 |
| MscI | TGGCCA | 1 cut(s) | 3032 |
| MseI | TTAA | 8 cut(s) | 324, 387, 1551, 1668, 2435, 2763, 2921, 3117 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2186 |
| Msp20I | TGGCCA | 1 cut(s) | 3032 |
| MspA1I | CMGCKG | 2 cut(s) | 995, 3178 |
| MspI | CCGG | 4 cut(s) | 592, 1388, 1802, 2828 |
| MspR9I | CCNGG | 6 cut(s) | 411, 1389, 1545, 2037, 3029, 3285 |
| MunI | CAATTG | 1 cut(s) | 1163 |
| Mva1269I | GAATGC | 1 cut(s) | 2560 |
| MvaI | CCWGG | 5 cut(s) | 411, 1545, 2037, 3029, 3285 |
| MvnI | CGCG | 1 cut(s) | 3178 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 29, 98, 605, 1927, 2505 |
| NciI | CCSGG | 1 cut(s) | 1389 |
| NcoI | CCATGG | 1 cut(s) | 2302 |
| NdeI | CATATG | 1 cut(s) | 2799 |
| NlaIV | GGNNCC | 7 cut(s) | 190, 687, 1309, 1393, 2041, 2814, 3135 |
| NmuCI | GTSAC | 8 cut(s) | 241, 486, 736, 1055, 2170, 2646, 2726, 3236 |
| NsiI | ATGCAT | 1 cut(s) | 2562 |
| NspI | RCATGY | 4 cut(s) | 1010, 1507, 1816, 2181 |
| PaeI | GCATGC | 1 cut(s) | 2181 |
| PagI | TCATGA | 4 cut(s) | 610, 1324, 1963, 2595 |
| PaqCI | CACCTGC | 1 cut(s) | 2641 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 574 |
| PctI | GAATGC | 1 cut(s) | 2560 |
| PfoI | TCCNGGA | 1 cut(s) | 1387 |
| PkrI | GCNGC | 8 cut(s) | 319, 460, 675, 763, 1012, 1177, 1312, 1932 |
| PleI | GAGTC | 3 cut(s) | 102, 1140, 2254 |
| PpsI | GAGTC | 3 cut(s) | 102, 1140, 2254 |
| PpuMI | RGGWCCY | 2 cut(s) | 686, 1534 |
| Psp1406I | AACGTT | 1 cut(s) | 370 |
| Psp5II | RGGWCCY | 2 cut(s) | 686, 1534 |
| Psp6I | CCWGG | 5 cut(s) | 409, 1543, 2035, 3027, 3283 |
| PspGI | CCWGG | 5 cut(s) | 409, 1543, 2035, 3027, 3283 |
| PspN4I | GGNNCC | 7 cut(s) | 190, 687, 1309, 1393, 2041, 2814, 3135 |
| PspPI | GGNCC | 9 cut(s) | 416, 686, 887, 1159, 1208, 1534, 1608, 1761, 2843 |
| PspPPI | RGGWCCY | 2 cut(s) | 686, 1534 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 141, 173 |
| PstI | CTGCAG | 1 cut(s) | 2642 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2483 |
| PsuI | RGATCY | 6 cut(s) | 188, 1067, 2021, 2039, 2092, 2234 |
| PvuII | CAGCTG | 1 cut(s) | 995 |
| RsaI | GTAC | 7 cut(s) | 166, 1769, 1956, 2146, 2449, 3174, 3289 |
| RsaNI | GTAC | 7 cut(s) | 165, 1768, 1955, 2145, 2448, 3173, 3288 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2186 |
| SacII | CCGCGG | 1 cut(s) | 3179 |
| SalI | GTCGAC | 1 cut(s) | 3243 |
| SaqAI | TTAA | 8 cut(s) | 324, 387, 1551, 1668, 2435, 2763, 2921, 3117 |
| SatI | GCNGC | 8 cut(s) | 318, 459, 674, 762, 1011, 1176, 1311, 1931 |
| Sau96I | GGNCC | 9 cut(s) | 416, 686, 887, 1159, 1208, 1534, 1608, 1761, 2843 |
| SchI | GAGTC | 3 cut(s) | 102, 1141, 2255 |
| ScrFI | CCNGG | 6 cut(s) | 411, 1389, 1545, 2037, 3029, 3285 |
| SduI | GDGCHC | 3 cut(s) | 1483, 2719, 2989 |
| SexAI | ACCWGGT | 2 cut(s) | 409, 3283 |
| SfaNI | GCATC | 9 cut(s) | 45, 578, 640, 748, 1388, 1686, 2386, 2547, 2730 |
| SfcI | CTRYAG | 7 cut(s) | 550, 990, 1359, 1702, 1819, 2607, 2638 |
| Sfr303I | CCGCGG | 1 cut(s) | 3179 |
| SgrBI | CCGCGG | 1 cut(s) | 3179 |
| SinI | GGWCC | 7 cut(s) | 416, 686, 887, 1208, 1534, 1608, 2843 |
| SmiI | ATTTAAAT | 1 cut(s) | 3118 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2186 |
| SmlI | CTYRAG | 3 cut(s) | 802, 1659, 2757 |
| SmoI | CTYRAG | 3 cut(s) | 802, 1659, 2757 |
| SphI | GCATGC | 1 cut(s) | 2181 |
| SsiI | CCGC | 8 cut(s) | 23, 458, 674, 1311, 3061, 3176, 3178, 3228 |
| SspI | AATATT | 2 cut(s) | 445, 3126 |
| StyD4I | CCNGG | 6 cut(s) | 409, 1387, 1543, 2035, 3027, 3283 |
| StyI | CCWWGG | 9 cut(s) | 1061, 1073, 1591, 1742, 2302, 2426, 2487, 2967, 3033 |
| SwaI | ATTTAAAT | 1 cut(s) | 3118 |
| TaaI | ACNGT | 9 cut(s) | 148, 164, 247, 258, 519, 1201, 1300, 2447, 3242 |
| TaiI | ACGT | 3 cut(s) | 373, 699, 2150 |
| TaqI | TCGA | 2 cut(s) | 1857, 3244 |
| TatI | WGTACW | 1 cut(s) | 164 |
| TauI | GCSGC | 3 cut(s) | 461, 676, 1313 |
| Tru1I | TTAA | 8 cut(s) | 324, 387, 1551, 1668, 2435, 2763, 2921, 3117 |
| Tru9I | TTAA | 8 cut(s) | 324, 387, 1551, 1668, 2435, 2763, 2921, 3117 |
| TscAI | CASTG | 5 cut(s) | 167, 524, 897, 2811, 2921 |
| TseFI | GTSAC | 8 cut(s) | 241, 486, 736, 1055, 2170, 2646, 2726, 3236 |
| TseI | GCWGC | 5 cut(s) | 317, 761, 1010, 1175, 1930 |
| Tsp45I | GTSAC | 8 cut(s) | 241, 486, 736, 1055, 2170, 2646, 2726, 3236 |
| TspGWI | ACGGA | 3 cut(s) | 566, 2475, 2745 |
| TspRI | CASTG | 5 cut(s) | 167, 524, 897, 2811, 2921 |
| VpaK11BI | GGWCC | 7 cut(s) | 416, 686, 887, 1208, 1534, 1608, 2843 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3032 |
| XbaI | TCTAGA | 3 cut(s) | 917, 1246, 2231 |
| XceI | RCATGY | 4 cut(s) | 1010, 1507, 1816, 2181 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 507 |
| XmaJI | CCTAGG | 2 cut(s) | 1061, 1742 |
| XmiI | GTMKAC | 1 cut(s) | 3244 |
| Zsp2I | ATGCAT | 1 cut(s) | 2562 |