FvH4_6g04681

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000587
Physical Location & Seq
Forward (+)
2586130 .. 2588111
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04681.t1

Sequence Viewer

Length: 1008 bp
ATGGAGAGGAACAAAGGGGAGGCAATCAGGGCAATGCAACTTTCAAAATTGAAGATTCAAGAGAATGATTTTGCTGGAGCAAGGAAAATGGCACGAGTAGCTCACCGACTCTTCCCTGAGGATGAGAAAATTTCTAAATTACTTGCACTCTCTGAAGTCTACTATTCAATTTTTGTGCTCGGTAAAGCTGTGGAACAGCGGCCTTTTAAAGCCGATCAAACATGGGTTCTTTATGATCCAGCAGATGGCATGCCAAGACGCTATGCATGTATCAGGAAAGTATTAGGCCCTGGATTTAAATTGAAAATCACCTGGCTGGAGCCTGATCCCGATGACCAAGGTGAGGTTGATTGGTGTAGCAAAAAGTTGCCAGTTGCATGTGGTAAGTTCGGACTTGCGTGCACTGAAGAAATTACAGATCATACTGTGTTTTCTCACGAGTATATACACCCCACAAAAAGCAAGGATAAAAACTCTTTTTGGATATATCCTAGGAAGGGAGAAACCTGGGCCCTCTACCAGGACTGGGATTTGGGGTGGAGTTCTGAGCCTGAAAAGCATTCACAATACAAGTTTGATTTTGTGGAACTCCTGTCAGATTTTTCTGAAAGGGGTGGTGCTAGAGTTTCTTATTTAGGAAAAGTAATAGGATTTGTTAGTTTGTTTGAGCCAGCTGAGCAGCATGGGGTTCTCAGGTTTCAAGTGTCACCTAATGAGCTGTATAGATTTTCTCATCAGATTCCCTCTTACAAGATGAGTGGTCGTGAAGGATATGGTGTTCCTAAAGGTTCTTTTAAGTTTGATCCTGCTTCTTTGCCTGCCAACCGAAGAAGCACCGTCACTGCAGCCAAACTATCTGGCTTTATACCTTCTGGTAGAATTCGCTCCGCTGTGAAACGGGTGAAGAAAAGTAGAGCTTGCCCTGCTGTGAAACAGGTGAAGAAAGATCCTGAAAGGGGGGAACGCAAGAACGGGAACATCAACAGTGAACCAATTGTGATAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

336

Amino Acids

38.15

Weight (kDa)

9.38

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 65 - 256 1.8e-66 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 245
AccI GTMKAC 1 cut(s) 159
AciI CCGC 2 cut(s) 199, 888
AclWI GGATC 4 cut(s) 230, 320, 797, 941
AcsI RAATTY 2 cut(s) 129, 879
AcuI CTGAAG 2 cut(s) 174, 426
AfiI CCNNNNNNNGG 6 cut(s) 245, 343, 497, 520, 526, 956
AgsI TTSAA 6 cut(s) 45, 52, 59, 168, 304, 701
AjnI CCWGG 4 cut(s) 289, 311, 506, 519
AloI GAACNNNNNNTCC 2 cut(s) 762, 794
AluBI AGCT 5 cut(s) 101, 188, 674, 718, 917
AluI AGCT 5 cut(s) 101, 188, 674, 718, 917
Alw21I GWGCWC 2 cut(s) 180, 404
Alw44I GTGCAC 1 cut(s) 400
AlwI GGATC 4 cut(s) 230, 320, 797, 941
AoxI GGCC 3 cut(s) 200, 286, 510
ApaI GGGCCC 1 cut(s) 514
ApaLI GTGCAC 1 cut(s) 400
ApeKI GCWGC 2 cut(s) 679, 845
ApoI RAATTY 2 cut(s) 129, 879
AspA2I CCTAGG 1 cut(s) 491
AspS9I GGNCC 3 cut(s) 287, 510, 511
AsuHPI GGTGA 6 cut(s) 95, 301, 353, 699, 913, 949
AvrII CCTAGG 1 cut(s) 491
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 2 cut(s) 404, 514
BanII GRGCYC 1 cut(s) 514
BauI CACGAG 2 cut(s) 93, 437
Bbv12I GWGCWC 2 cut(s) 180, 404
BbvI GCAGC 2 cut(s) 691, 857
BccI CCATC 1 cut(s) 239
BciT130I CCWGG 4 cut(s) 291, 313, 508, 521
BfaI CTAG 3 cut(s) 492, 621, 1006
BfmI CTRYAG 1 cut(s) 843
BisI GCNGC 3 cut(s) 200, 680, 846
BlnI CCTAGG 1 cut(s) 491
BlpI GCTNAGC 1 cut(s) 675
BlsI GCNGC 3 cut(s) 201, 681, 847
Bme1390I CCNGG 4 cut(s) 291, 313, 508, 521
BmgT120I GGNCC 3 cut(s) 287, 510, 511
BmiI GGNNCC 2 cut(s) 321, 512
BmrFI CCNGG 4 cut(s) 291, 313, 508, 521
BmrI ACTGGG 1 cut(s) 535
BmuI ACTGGG 1 cut(s) 535
BpmI CTGGAG 2 cut(s) 96, 338
Bpu1102I GCTNAGC 1 cut(s) 675
BsaJI CCNNGG 4 cut(s) 289, 337, 491, 507
Bsc4I CCNNNNNNNGG 6 cut(s) 245, 343, 497, 520, 526, 956
Bse1I ACTGG 2 cut(s) 371, 530
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 39
BseBI CCWGG 4 cut(s) 291, 313, 508, 521
BseDI CCNNGG 4 cut(s) 289, 337, 491, 507
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 6 cut(s) 245, 343, 497, 520, 526, 956
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 4 cut(s) 108, 537, 666, 706
BseNI ACTGG 2 cut(s) 371, 530
BseSI GKGCMC 2 cut(s) 404, 514
BseXI GCAGC 2 cut(s) 691, 857
BshFI GGCC 3 cut(s) 202, 288, 512
BsiHKAI GWGCWC 2 cut(s) 180, 404
BslI CCNNNNNNNGG 6 cut(s) 245, 343, 497, 520, 526, 956
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 3 cut(s) 202, 288, 512
Bsp120I GGGCCC 1 cut(s) 510
Bsp1286I GDGCHC 3 cut(s) 180, 404, 514
Bsp143I GATC 6 cut(s) 214, 235, 325, 418, 802, 946
Bsp1720I GCTNAGC 1 cut(s) 675
BspACI CCGC 2 cut(s) 199, 888
BspANI GGCC 3 cut(s) 202, 288, 512
BspCNI CTCAG 4 cut(s) 109, 538, 667, 705
BspLI GGNNCC 2 cut(s) 321, 512
BspMAI CTGCAG 1 cut(s) 847
BspPI GGATC 4 cut(s) 230, 320, 797, 941
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 2 cut(s) 371, 530
BssECI CCNNGG 4 cut(s) 289, 337, 491, 507
BssMI GATC 6 cut(s) 214, 235, 325, 418, 802, 946
BssSI CACGAG 2 cut(s) 93, 437
BssT1I CCWWGG 2 cut(s) 337, 491
Bst2BI CACGAG 2 cut(s) 93, 437
Bst2UI CCWGG 4 cut(s) 291, 313, 508, 521
Bst4CI ACNGT 3 cut(s) 427, 838, 986
Bst6I CTCTTC 1 cut(s) 116
BstC8I GCNNGC 5 cut(s) 251, 400, 672, 819, 919
BstDEI CTNAG 4 cut(s) 117, 546, 675, 692
BstENI CCTNNNNNAGG 1 cut(s) 518
BstF5I GGATG 1 cut(s) 127
BstKTI GATC 6 cut(s) 217, 238, 328, 421, 805, 949
BstMBI GATC 6 cut(s) 214, 235, 325, 418, 802, 946
BstMWI GCNNNNNNNGC 5 cut(s) 29, 98, 556, 676, 923
BstNI CCWGG 4 cut(s) 291, 313, 508, 521
BstNSI RCATGY 3 cut(s) 253, 270, 381
BstSCI CCNGG 4 cut(s) 289, 311, 506, 519
BstSFI CTRYAG 1 cut(s) 843
BstSLI GKGCMC 2 cut(s) 404, 514
BstV1I GCAGC 2 cut(s) 691, 857
BstX2I RGATCY 1 cut(s) 946
BstYI RGATCY 1 cut(s) 946
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 3 cut(s) 202, 288, 512
BtsCI GGATG 1 cut(s) 127
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 3 cut(s) 402, 840, 991
Cac8I GCNNGC 5 cut(s) 251, 400, 672, 819, 919
Cfr13I GGNCC 3 cut(s) 287, 510, 511
CseI GACGC 1 cut(s) 267
CspCI CAANNNNNGTGG 2 cut(s) 739, 774
CviAII CATG 5 cut(s) 222, 250, 267, 378, 683
DdeI CTNAG 4 cut(s) 117, 546, 675, 692
DpnI GATC 6 cut(s) 216, 237, 327, 420, 804, 948
DpnII GATC 6 cut(s) 214, 235, 325, 418, 802, 946
DraI TTTAAA 2 cut(s) 208, 298
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco130I CCWWGG 2 cut(s) 337, 491
Eco24I GRGCYC 1 cut(s) 514
Eco57I CTGAAG 2 cut(s) 174, 426
Eco81I CCTNAGG 1 cut(s) 117
EcoNI CCTNNNNNAGG 1 cut(s) 518
EcoO109I RGGNCCY 2 cut(s) 287, 511
EcoRI GAATTC 1 cut(s) 879
EcoRII CCWGG 4 cut(s) 289, 311, 506, 519
EcoT14I CCWWGG 2 cut(s) 337, 491
EcoT22I ATGCAT 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 514
ErhI CCWWGG 2 cut(s) 337, 491
FaeI CATG 5 cut(s) 225, 253, 270, 381, 686
FalI AAGNNNNNCTT 2 cut(s) 901, 933
FatI CATG 5 cut(s) 221, 249, 266, 377, 682
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 3 cut(s) 200, 680, 846
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 3 cut(s) 200, 680, 846
FspBI CTAG 3 cut(s) 492, 621, 1006
GluI GCNGC 3 cut(s) 200, 680, 846
GsuI CTGGAG 2 cut(s) 96, 338
HaeIII GGCC 3 cut(s) 202, 288, 512
HgaI GACGC 1 cut(s) 267
Hin1II CATG 5 cut(s) 225, 253, 270, 381, 686
HinfI GANTC 3 cut(s) 55, 108, 739
HphI GGTGA 6 cut(s) 95, 301, 353, 699, 913, 949
Hpy166II GTNNAC 3 cut(s) 160, 402, 989
Hpy188I TCNGA 6 cut(s) 154, 392, 547, 598, 607, 738
Hpy188III TCNNGA 6 cut(s) 59, 274, 329, 437, 764, 950
Hpy8I GTNNAC 3 cut(s) 160, 402, 989
HpyAV CCTTC 3 cut(s) 490, 761, 879
HpyCH4III ACNGT 3 cut(s) 427, 838, 986
HpyCH4V TGCA 6 cut(s) 37, 146, 266, 377, 402, 845
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 98, 556, 676, 923
HpyF3I CTNAG 4 cut(s) 117, 546, 675, 692
Hsp92II CATG 5 cut(s) 225, 253, 270, 381, 686
Kzo9I GATC 6 cut(s) 214, 235, 325, 418, 802, 946
LmnI GCTCC 3 cut(s) 77, 319, 890
Lsp1109I GCAGC 2 cut(s) 691, 857
MaeI CTAG 3 cut(s) 492, 621, 1006
MaeIII GTNAC 2 cut(s) 705, 838
MalI GATC 6 cut(s) 216, 237, 327, 420, 804, 948
MboI GATC 6 cut(s) 214, 235, 325, 418, 802, 946
MboII GAAGA 6 cut(s) 64, 103, 419, 840, 916, 952
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 3 cut(s) 180, 404, 514
MluCI AATT 8 cut(s) 47, 129, 137, 168, 299, 411, 879, 993
MlyI GAGTC 1 cut(s) 102
MnlI CCTC 5 cut(s) 13, 112, 337, 524, 754
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 3 cut(s) 207, 297, 795
MspA1I CMGCKG 3 cut(s) 199, 674, 890
MspR9I CCNGG 4 cut(s) 291, 313, 508, 521
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 4 cut(s) 291, 313, 508, 521
MwoI GCNNNNNNNGC 5 cut(s) 29, 98, 556, 676, 923
NdeII GATC 6 cut(s) 214, 235, 325, 418, 802, 946
NlaIII CATG 5 cut(s) 225, 253, 270, 381, 686
NlaIV GGNNCC 2 cut(s) 321, 512
NmuCI GTSAC 2 cut(s) 705, 838
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 3 cut(s) 253, 270, 381
PaeI GCATGC 1 cut(s) 253
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 2 cut(s) 55, 739
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 3 cut(s) 201, 681, 847
PleI GAGTC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 102
Psp6I CCWGG 4 cut(s) 289, 311, 506, 519
PspGI CCWGG 4 cut(s) 289, 311, 506, 519
PspN4I GGNNCC 2 cut(s) 321, 512
PspOMI GGGCCC 1 cut(s) 510
PspPI GGNCC 3 cut(s) 287, 510, 511
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 946
PvuII CAGCTG 1 cut(s) 674
SaqAI TTAA 3 cut(s) 207, 297, 795
SatI GCNGC 3 cut(s) 200, 680, 846
Sau3AI GATC 6 cut(s) 214, 235, 325, 418, 802, 946
Sau96I GGNCC 3 cut(s) 287, 510, 511
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 4 cut(s) 291, 313, 508, 521
SduI GDGCHC 3 cut(s) 180, 404, 514
SfcI CTRYAG 1 cut(s) 843
SmiI ATTTAAAT 1 cut(s) 298
SphI GCATGC 1 cut(s) 253
Sse9I AATT 8 cut(s) 47, 129, 137, 168, 299, 411, 879, 993
SsiI CCGC 2 cut(s) 199, 888
SspMI CTAG 3 cut(s) 492, 621, 1006
StyD4I CCNGG 4 cut(s) 289, 311, 506, 519
StyI CCWWGG 2 cut(s) 337, 491
SwaI ATTTAAAT 1 cut(s) 298
TaaI ACNGT 3 cut(s) 427, 838, 986
TasI AATT 8 cut(s) 47, 129, 137, 168, 299, 411, 879, 993
TauI GCSGC 1 cut(s) 202
TfiI GAWTC 2 cut(s) 55, 739
Tru1I TTAA 3 cut(s) 207, 297, 795
Tru9I TTAA 3 cut(s) 207, 297, 795
TscAI CASTG 3 cut(s) 409, 847, 991
TseFI GTSAC 2 cut(s) 705, 838
TseI GCWGC 2 cut(s) 679, 845
Tsp45I GTSAC 2 cut(s) 705, 838
TspRI CASTG 3 cut(s) 409, 847, 991
Van91I CCANNNNNTGG 1 cut(s) 245
VneI GTGCAC 1 cut(s) 400
XagI CCTNNNNNAGG 1 cut(s) 518
XapI RAATTY 2 cut(s) 129, 879
XceI RCATGY 3 cut(s) 253, 270, 381
XmaJI CCTAGG 1 cut(s) 491
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 3 cut(s) 492, 621, 1006
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.