FvH4_3g11611

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000882
Physical Location & Seq
Forward (+)
6854756 .. 6859890
5135 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11611.t1

Sequence Viewer

Length: 2694 bp
ATGGAAAGACACAGATCAAACTATACTCCAAAAGCAATATTGTTTCATCATCTTGTACCATTGTTCTTCTTCTTCTTTTCTCTGCTCGAGTGTTTTGTTGCAGAAGAGCCAGTTGGGTATGGATATAGAGTGGAGTCTGTGAACTCTGATCCCTCTGGGAAGACAGTGACAGCTAGTCTTGGCCTGATCAACAGCTCCTTGGTCTATGGACCTGACATCCCAAATCTCAAGCTTTATGCCAGCTATGAGACCAAGGAAAGACTAAGAATTCGAATAATAGACTCGGATAACCAAAGATGGGAAATCCCACAAGACATCCTCCCCCACCAAACTCCACAAACATCCCACCATTCCATCTCCGAAAACGACTTCATTTTCACCCTCCATAACACCACCCCCTTCGGCTTCACCGTCACCCGCCGCTCCTCCTCCGAAGTCGTTTTCGACACCACCCCAAACCCCTCCGACCCTTCCTCTATCTTTGTCTTCAAGGACCAGTACATCCAGCTTTCCTCGTCTCTCCCGGAAACCCGGTCCTCCCTCTACGGCCTAGGAGAACACACCAAACCCTCCTTCAAACTCCAACCCAATCAGACTCTCACACTCTGGACTGCCGACATTGGCAGCGCCAACCCCGACGTCAACCTCTATGGGTCCCACCCCTTCTACATGGACGTCAGGTCGCCGTCCGGCGACAATGGAAAAGTTACAGCGGGAGCTACTCATGGAGTGTTACTGTTGAATAGTAATGGCATGGATGTAAATTATGGCGGTGACAGGGTTACTTATAAGGTGATTGGTGGAGTTGTTGACTTGTATTTCTTCTCCGGTCCGACGCCGGAATTGGTGATGGAGCAGTACACGGAGCTCATTGGCCGACCTGCCCCCATGCCCTACTGGTCTTTTGGATTTCACCAGTGTAGATATGGTTACAAGGATGTTGCTGATCTTGAAGGAGTGGTTGCTGGTTATGCAAATGCTAGGATCCCTCTTGAAGTTATGTGGACTGACATTGACTACATGGATGCTTACAAGGATTTCACTCTTGATCCCATCAACTTTCCATTGGATAAGATGCAGAACTTCACAAACACCCTCCACCAAAATGGCCAGAAATATGTGCTCATCTTGGACCCTGGTATTAGCATAAATGAGAGCTATGCAACTTACATCAGAGGGAAGGCAGCAGATATATACATAAAACGTGATGGCATTCCTTACCAGGGAAATGTATGGCCTGGTGATGTTTACTATCCTGACTTTGTACATCCACAAAGTGAACAATTTTGGGCTAATGAGATCAAACTATTTCAAGACCAACTACCTTTTGATGGTCTCTGGCTTGATATGAATGAGGTATCCAATTTTATAACATCTCCACCCACTTTGAATTCCACACTTGATGATCCACCTTACAAAATTAATGATTCCGGAGTCCAGCGTCCGATCATTAGTAAGACTGTTCCGGCATCAGCTCTACACTTTGGTAATTTAACAGAGTATAATGTGCATAACCTTTATGGATTCTTGGAATCTAGAGCAACACATCAGGGGTTGATCAATGTTACTGGTAAGAGACCATTTATACTTACCAGATCAACCTTTGTGAGCTCTGGTAAGTACGCTGCACACTGGACTGGAGACAATGCAGCAAGATGGAGTGACTTGGCATACACAATTCCAGGCATTTTGAATTTTGGAATCTTTGGAGTTCCAATGGTTGGAGCTGATATATGTGGTTTCTCTTTGAATACTACTGAAGAACTCTGCCGGAGATGGATTCAGCTAGGGGCCTTTTACCCCTTTTCAAGAGATCATTCAGAGAAGTTTACGATCCGCCAAGAGCTCTATGTTTGGGATTCAGTGGCTGCATCAGCAAGGAAGGTACTAGGCCTCCGTTACAGGTTGCTCCCCTTGTTCTACACATCAATGTATCAAGCACACAAAAAAGGGACTCCCATAGCGAGGCCTCTTTTCTTCTCATTTCCTGAAGATACCAATACATACGACATCAGTTCACAGTTCTTGATTGGTAGAGGTGTAATGGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTGATGCATATTTTCCTACTGGAAACTGGTTTGATCTCTTTAACTACTCGAGATCAGTTAGTGTACACTCCGGAGAGTATGTTACATTAGACGCACCGCCTGATCATATCAATGTACATATCCGTGAAGGAAACATTCTGGCCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCTCGAAAGACTGCATTCGAACTTTTGGTGGTCATTAGTAGTAGCGGAGAGAGCAGTGGAGAAGTATTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTGTAGTGAAATTCTATTGTGGAGCAGCAAATGGAAGTGTATTTTTGAGATCAATGCTTGAAAATGGAGGGTTTGCATTGAGCCAAAAATGGATCATAGATAAAATTACCCTCATTGGGTTGGAAAACGTTGATGGATTAGAAGGGTTTGCAGTCAACATTACTGAAGGAACAAATTTGAAAGGAAAGTCGGTTGTCAAGGCCAACTTTCACAGTGATAAGCGTTTTTTCATGGTAGAAATATCCAGTGTCTCCATTCTTATAGGAAAAGAGTTTGAGTTGGAGCTGAGGCTAAAGCATGACACCGGTGAAGACACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

898

Amino Acids

100.51

Weight (kDa)

5.32

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 88 - 228 8.6e-11 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 294 - 642 5.6e-121 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 651 - 740 3.9e-25 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 789, 1370
AatII GACGTC 2 cut(s) 642, 678
Acc36I ACCTGC 1 cut(s) 889
AccB7I CCANNNNNTGG 1 cut(s) 1721
AccBSI CCGCTC 1 cut(s) 423
AccIII TCCGGA 2 cut(s) 1430, 2150
AciI CCGC 7 cut(s) 418, 421, 713, 771, 1837, 2177, 2298
AclI AACGTT 1 cut(s) 2499
AclWI GGATC 7 cut(s) 143, 979, 992, 1043, 1400, 1828, 2471
AcoI YGGCCR 2 cut(s) 874, 1108
AcsI RAATTY 6 cut(s) 267, 1390, 1693, 2074, 2381, 2545
AcuI CTGAAG 3 cut(s) 1779, 2010, 2556
AcyI GRCGYC 3 cut(s) 639, 675, 836
AdeI CACNNNGTG 1 cut(s) 1277
AfaI GTAC 8 cut(s) 57, 500, 860, 1266, 1622, 1887, 2145, 2196
AfiI CCNNNNNNNGG 6 cut(s) 298, 1223, 1331, 1721, 1965, 2487
AgeI ACCGGT 1 cut(s) 2675
AhdI GACNNNNNGTC 2 cut(s) 174, 679
AjnI CCWGG 4 cut(s) 1135, 1221, 1237, 1681
AjuI GAANNNNNNNTTGG 4 cut(s) 96, 128, 557, 589
Alw21I GWGCWC 4 cut(s) 870, 1125, 1613, 1848
Alw26I GTCTC 6 cut(s) 242, 522, 1340, 1570, 1635, 2626
AlwI GGATC 7 cut(s) 143, 979, 992, 1043, 1400, 1828, 2471
AlwNI CAGNNNCTG 1 cut(s) 1868
Ama87I CYCGRG 2 cut(s) 86, 2128
Aor13HI TCCGGA 2 cut(s) 1430, 2150
ApeKI GCWGC 8 cut(s) 624, 1184, 1625, 1649, 1868, 2062, 2252, 2396
ApoI RAATTY 6 cut(s) 267, 1390, 1693, 2074, 2381, 2545
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
AseI ATTAAT 1 cut(s) 1422
AsiGI ACCGGT 1 cut(s) 2675
AspA2I CCTAGG 1 cut(s) 550
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 7 cut(s) 209, 493, 534, 654, 830, 1132, 1791
AsuC2I CCSGG 2 cut(s) 524, 532
AsuII TTCGAA 2 cut(s) 271, 2271
AvaI CYCGRG 2 cut(s) 86, 2128
AvaII GGWCC 6 cut(s) 209, 493, 534, 654, 830, 1132
AvrII CCTAGG 1 cut(s) 550
BalI TGGCCA 1 cut(s) 1110
BamHI GGATCC 1 cut(s) 984
BanII GRGCYC 3 cut(s) 870, 1613, 1848
BbsI GAAGAC 3 cut(s) 167, 478, 2688
Bbv12I GWGCWC 4 cut(s) 870, 1125, 1613, 1848
BbvCI CCTCAGC 1 cut(s) 2657
BbvI GCAGC 8 cut(s) 636, 1196, 1612, 1661, 1855, 2074, 2264, 2408
BceAI ACGGC 2 cut(s) 562, 670
BciT130I CCWGG 4 cut(s) 1137, 1223, 1239, 1683
BciVI GTATCC 1 cut(s) 1369
BclI TGATCA 3 cut(s) 186, 1557, 2182
BcnI CCSGG 2 cut(s) 524, 532
BcoDI GTCTC 6 cut(s) 242, 522, 1340, 1570, 1635, 2626
BfaI CTAG 6 cut(s) 174, 551, 981, 1536, 1787, 1889
BfoI RGCGCY 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 889
BfuI GTATCC 1 cut(s) 1369
BisI GCNGC 9 cut(s) 421, 625, 1185, 1626, 1650, 1869, 2063, 2253, 2397
BlnI CCTAGG 1 cut(s) 550
BlsI GCNGC 9 cut(s) 422, 626, 1186, 1627, 1651, 1870, 2064, 2254, 2398
Bme1390I CCNGG 6 cut(s) 524, 532, 1137, 1223, 1239, 1683
Bme18I GGWCC 6 cut(s) 209, 493, 534, 654, 830, 1132
BmeRI GACNNNNNGTC 2 cut(s) 174, 679
BmeT110I CYCGRG 2 cut(s) 86, 2128
BmgT120I GGNCC 7 cut(s) 209, 493, 534, 654, 830, 1132, 1791
BmiI GGNNCC 5 cut(s) 655, 656, 986, 1134, 1792
BmrFI CCNGG 6 cut(s) 524, 532, 1137, 1223, 1239, 1683
BmsI GCATC 5 cut(s) 1015, 1065, 1478, 1880, 2074
BpiI GAAGAC 3 cut(s) 167, 478, 2688
BpmI CTGGAG 1 cut(s) 1659
Bpu10I CCTNAGC 1 cut(s) 2657
Bpu14I TTCGAA 2 cut(s) 271, 2271
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 2 cut(s) 524, 532
BsaHI GRCGYC 3 cut(s) 639, 675, 836
BsaI GGTCTC 3 cut(s) 242, 1340, 1570
BsaJI CCNNGG 5 cut(s) 198, 252, 550, 1135, 1222
BsaWI WCCGGW 4 cut(s) 827, 1430, 2150, 2675
BsaXI ACNNNNNCTCC 6 cut(s) 1878, 1908, 2145, 2175, 2325, 2355
Bsc4I CCNNNNNNNGG 6 cut(s) 298, 1223, 1331, 1721, 1965, 2487
Bse118I RCCGGY 1 cut(s) 2675
BseAI TCCGGA 2 cut(s) 1430, 2150
BseBI CCWGG 4 cut(s) 1137, 1223, 1239, 1683
BseDI CCNNGG 5 cut(s) 198, 252, 550, 1135, 1222
BseGI GGATG 9 cut(s) 216, 315, 341, 501, 763, 943, 1030, 1267, 2332
BseLI CCNNNNNNNGG 6 cut(s) 298, 1223, 1331, 1721, 1965, 2487
BseMII CTCAG 1 cut(s) 2648
BseRI GAGGAG 3 cut(s) 415, 418, 2367
BseXI GCAGC 8 cut(s) 636, 1196, 1612, 1661, 1855, 2074, 2264, 2408
BsgI GTGCAG 1 cut(s) 1611
BshTI ACCGGT 1 cut(s) 2675
BsiHKAI GWGCWC 4 cut(s) 870, 1125, 1613, 1848
BsiHKCI CYCGRG 2 cut(s) 86, 2128
BslFI GGGAC 2 cut(s) 640, 1966
BslI CCNNNNNNNGG 6 cut(s) 298, 1223, 1331, 1721, 1965, 2487
BsmAI GTCTC 6 cut(s) 242, 522, 1340, 1570, 1635, 2626
BsmBI CGTCTC 1 cut(s) 522
BsmFI GGGAC 2 cut(s) 640, 1966
BsmI GAATGC 2 cut(s) 1212, 2267
Bso31I GGTCTC 3 cut(s) 242, 1340, 1570
BsoBI CYCGRG 2 cut(s) 86, 2128
Bsp119I TTCGAA 2 cut(s) 271, 2271
Bsp1286I GDGCHC 4 cut(s) 870, 1125, 1613, 1848
Bsp13I TCCGGA 2 cut(s) 1430, 2150
Bsp1407I TGTACA 3 cut(s) 1264, 2143, 2194
BspACI CCGC 7 cut(s) 418, 421, 713, 771, 1837, 2177, 2298
BspCNI CTCAG 1 cut(s) 2649
BspEI TCCGGA 2 cut(s) 1430, 2150
BspLI GGNNCC 5 cut(s) 655, 656, 986, 1134, 1792
BspMI ACCTGC 1 cut(s) 889
BspPI GGATC 7 cut(s) 143, 979, 992, 1043, 1400, 1828, 2471
BspQI GCTCTTC 1 cut(s) 99
BspT104I TTCGAA 2 cut(s) 271, 2271
BspTNI GGTCTC 3 cut(s) 242, 1340, 1570
BsrBI CCGCTC 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 2675
BsrGI TGTACA 3 cut(s) 1264, 2143, 2194
BssAI RCCGGY 1 cut(s) 2675
BssECI CCNNGG 5 cut(s) 198, 252, 550, 1135, 1222
BssNI GRCGYC 3 cut(s) 639, 675, 836
BssT1I CCWWGG 3 cut(s) 198, 252, 550
Bst2UI CCWGG 4 cut(s) 1137, 1223, 1239, 1683
Bst4CI ACNGT 6 cut(s) 166, 412, 738, 1462, 2022, 2585
Bst6I CTCTTC 1 cut(s) 99
BstACI GRCGYC 3 cut(s) 639, 675, 836
BstAUI TGTACA 3 cut(s) 1264, 2143, 2194
BstBI TTCGAA 2 cut(s) 271, 2271
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 2 cut(s) 263, 2657
BstF5I GGATG 9 cut(s) 216, 315, 341, 501, 763, 943, 1030, 1267, 2332
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 1 cut(s) 629
BstMAI GTCTC 6 cut(s) 242, 522, 1340, 1570, 1635, 2626
BstMWI GCNNNNNNNGC 3 cut(s) 971, 1874, 2304
BstNI CCWGG 4 cut(s) 1137, 1223, 1239, 1683
BstSCI CCNGG 6 cut(s) 522, 530, 1135, 1221, 1237, 1681
BstV1I GCAGC 8 cut(s) 636, 1196, 1612, 1661, 1855, 2074, 2264, 2408
BstV2I GAAGAC 3 cut(s) 167, 478, 2688
BstX2I RGATCY 1 cut(s) 984
BstXI CCANNNNNNTGG 1 cut(s) 1106
BstYI RGATCY 1 cut(s) 984
BsuI GTATCC 1 cut(s) 1369
BtsCI GGATG 9 cut(s) 216, 315, 341, 501, 763, 943, 1030, 1267, 2332
BtsI GCAGTG 1 cut(s) 2314
BtsIMutI CAGTG 7 cut(s) 171, 923, 1630, 1869, 2314, 2590, 2623
BveI ACCTGC 1 cut(s) 889
Cac8I GCNNGC 1 cut(s) 241
CaiI CAGNNNCTG 1 cut(s) 1868
CfoI GCGC 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 2675
Cfr13I GGNCC 7 cut(s) 209, 493, 534, 654, 830, 1132, 1791
CpoI CGGWCCG 1 cut(s) 830
CseI GACGC 3 cut(s) 844, 1430, 2180
Csp6I GTAC 8 cut(s) 56, 499, 859, 1265, 1621, 1886, 2144, 2195
CspAI ACCGGT 1 cut(s) 2675
CspCI CAANNNNNGTGG 2 cut(s) 1368, 1403
CspI CGGWCCG 1 cut(s) 830
CviAII CATG 7 cut(s) 670, 725, 754, 889, 1021, 2602, 2669
CviQI GTAC 8 cut(s) 56, 499, 859, 1265, 1621, 1886, 2144, 2195
DdeI CTNAG 2 cut(s) 263, 2657
DraIII CACNNNGTG 1 cut(s) 1277
DriI GACNNNNNGTC 2 cut(s) 174, 679
EaeI YGGCCR 2 cut(s) 874, 1108
Eam1104I CTCTTC 1 cut(s) 99
Eam1105I GACNNNNNGTC 2 cut(s) 174, 679
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 1826
Ecl136II GAGCTC 3 cut(s) 868, 1611, 1846
Eco130I CCWWGG 3 cut(s) 198, 252, 550
Eco147I AGGCCT 3 cut(s) 1893, 1969, 2237
Eco24I GRGCYC 3 cut(s) 870, 1613, 1848
Eco31I GGTCTC 3 cut(s) 242, 1340, 1570
Eco47I GGWCC 6 cut(s) 209, 493, 534, 654, 830, 1132
Eco53kI GAGCTC 3 cut(s) 868, 1611, 1846
Eco57I CTGAAG 3 cut(s) 1779, 2010, 2556
Eco88I CYCGRG 2 cut(s) 86, 2128
EcoICRI GAGCTC 3 cut(s) 868, 1611, 1846
EcoO109I RGGNCCY 2 cut(s) 654, 1791
EcoRI GAATTC 2 cut(s) 267, 1390
EcoRII CCWGG 4 cut(s) 1135, 1221, 1237, 1681
EcoT14I CCWWGG 3 cut(s) 198, 252, 550
EcoT22I ATGCAT 1 cut(s) 2089
EcoT38I GRGCYC 3 cut(s) 870, 1613, 1848
ErhI CCWWGG 3 cut(s) 198, 252, 550
Esp3I CGTCTC 1 cut(s) 522
FaeI CATG 7 cut(s) 673, 728, 757, 892, 1024, 2605, 2672
FalI AAGNNNNNCTT 3 cut(s) 2561, 2593, 2673
FaqI GGGAC 2 cut(s) 640, 1966
FatI CATG 7 cut(s) 669, 724, 753, 888, 1020, 2601, 2668
FauI CCCGC 2 cut(s) 425, 706
FbaI TGATCA 3 cut(s) 186, 1557, 2182
Fnu4HI GCNGC 9 cut(s) 421, 625, 1185, 1626, 1650, 1869, 2063, 2253, 2397
FokI GGATG 9 cut(s) 203, 302, 328, 488, 770, 950, 1037, 1254, 2339
FriOI GRGCYC 3 cut(s) 870, 1613, 1848
Fsp4HI GCNGC 9 cut(s) 421, 625, 1185, 1626, 1650, 1869, 2063, 2253, 2397
FspBI CTAG 6 cut(s) 174, 551, 981, 1536, 1787, 1889
GlaI GCGC 1 cut(s) 628
GluI GCNGC 9 cut(s) 421, 625, 1185, 1626, 1650, 1869, 2063, 2253, 2397
GsuI CTGGAG 1 cut(s) 1659
HaeII RGCGCY 1 cut(s) 630
HgaI GACGC 3 cut(s) 844, 1430, 2180
HhaI GCGC 1 cut(s) 629
Hin1I GRCGYC 3 cut(s) 639, 675, 836
Hin1II CATG 7 cut(s) 673, 728, 757, 892, 1024, 2605, 2672
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 3 cut(s) 643, 811, 2527
HindII GTYRAC 3 cut(s) 643, 811, 2527
HindIII AAGCTT 1 cut(s) 230
Hpy99I CGWCG 2 cut(s) 641, 838
HpyCH4III ACNGT 6 cut(s) 166, 412, 738, 1462, 2022, 2585
HpyCH4IV ACGT 4 cut(s) 639, 675, 1204, 2499
HpyF10VI GCNNNNNNNGC 3 cut(s) 971, 1874, 2304
HpyF3I CTNAG 2 cut(s) 263, 2657
HpySE526I ACGT 4 cut(s) 639, 675, 1204, 2499
Hsp92I GRCGYC 3 cut(s) 639, 675, 836
Hsp92II CATG 7 cut(s) 673, 728, 757, 892, 1024, 2605, 2672
HspAI GCGC 1 cut(s) 627
KflI GGGWCCC 1 cut(s) 654
Kpn2I TCCGGA 2 cut(s) 1430, 2150
Ksp22I TGATCA 3 cut(s) 186, 1557, 2182
LguI GCTCTTC 1 cut(s) 99
Lsp1109I GCAGC 8 cut(s) 636, 1196, 1612, 1661, 1855, 2074, 2264, 2408
LweI GCATC 5 cut(s) 1015, 1065, 1478, 1880, 2074
MaeI CTAG 6 cut(s) 174, 551, 981, 1536, 1787, 1889
MaeII ACGT 4 cut(s) 639, 675, 1204, 2499
MbiI CCGCTC 1 cut(s) 423
MflI RGATCY 1 cut(s) 984
MhlI GDGCHC 4 cut(s) 870, 1125, 1613, 1848
MlsI TGGCCA 1 cut(s) 1110
MluNI TGGCCA 1 cut(s) 1110
MlyI GAGTC 5 cut(s) 143, 275, 589, 1443, 1948
MmeI TCCRAC 6 cut(s) 489, 607, 857, 1702, 2472, 2631
Mox20I TGGCCA 1 cut(s) 1110
Mph1103I ATGCAT 1 cut(s) 2089
MroI TCCGGA 2 cut(s) 1430, 2150
MscI TGGCCA 1 cut(s) 1110
MseI TTAA 4 cut(s) 1422, 1493, 2121, 2692
MslI CAYNNNNRTG 4 cut(s) 1104, 1928, 2190, 2202
Msp20I TGGCCA 1 cut(s) 1110
MspA1I CMGCKG 1 cut(s) 713
MspR9I CCNGG 6 cut(s) 524, 532, 1137, 1223, 1239, 1683
Mva1269I GAATGC 2 cut(s) 1212, 2267
MvaI CCWGG 4 cut(s) 1137, 1223, 1239, 1683
MwoI GCNNNNNNNGC 3 cut(s) 971, 1874, 2304
NciI CCSGG 2 cut(s) 524, 532
NlaIII CATG 7 cut(s) 673, 728, 757, 892, 1024, 2605, 2672
NlaIV GGNNCC 5 cut(s) 655, 656, 986, 1134, 1792
NmuCI GTSAC 5 cut(s) 166, 412, 773, 1661, 2049
NsiI ATGCAT 1 cut(s) 2089
NspV TTCGAA 2 cut(s) 271, 2271
PaeR7I CTCGAG 2 cut(s) 86, 2128
PceI AGGCCT 3 cut(s) 1893, 1969, 2237
PciSI GCTCTTC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PctI GAATGC 2 cut(s) 1212, 2267
PfeI GAWTC 6 cut(s) 1427, 1524, 1532, 1701, 1780, 1859
PflMI CCANNNNNTGG 1 cut(s) 1721
PfoI TCCNGGA 1 cut(s) 522
PinAI ACCGGT 1 cut(s) 2675
PkrI GCNGC 9 cut(s) 422, 626, 1186, 1627, 1651, 1870, 2064, 2254, 2398
PleI GAGTC 5 cut(s) 142, 275, 589, 1442, 1948
PpsI GAGTC 5 cut(s) 142, 275, 589, 1442, 1948
PpuMI RGGWCCY 1 cut(s) 654
PshBI ATTAAT 1 cut(s) 1422
PsiI TTATAA 2 cut(s) 789, 1370
Psp124BI GAGCTC 3 cut(s) 870, 1613, 1848
Psp1406I AACGTT 1 cut(s) 2499
Psp5II RGGWCCY 1 cut(s) 654
Psp6I CCWGG 4 cut(s) 1135, 1221, 1237, 1681
PspGI CCWGG 4 cut(s) 1135, 1221, 1237, 1681
PspN4I GGNNCC 5 cut(s) 655, 656, 986, 1134, 1792
PspPI GGNCC 7 cut(s) 209, 493, 534, 654, 830, 1132, 1791
PspPPI RGGWCCY 1 cut(s) 654
PspXI VCTCGAGB 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 1868
PsuI RGATCY 1 cut(s) 984
RsaI GTAC 8 cut(s) 57, 500, 860, 1266, 1622, 1887, 2145, 2196
RsaNI GTAC 8 cut(s) 56, 499, 859, 1265, 1621, 1886, 2144, 2195
RseI CAYNNNNRTG 4 cut(s) 1104, 1928, 2190, 2202
Rsr2I CGGWCCG 1 cut(s) 830
RsrII CGGWCCG 1 cut(s) 830
SacI GAGCTC 3 cut(s) 870, 1613, 1848
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 4 cut(s) 1422, 1493, 2121, 2692
SatI GCNGC 9 cut(s) 421, 625, 1185, 1626, 1650, 1869, 2063, 2253, 2397
Sau96I GGNCC 7 cut(s) 209, 493, 534, 654, 830, 1132, 1791
SchI GAGTC 5 cut(s) 143, 275, 589, 1443, 1948
ScrFI CCNGG 6 cut(s) 524, 532, 1137, 1223, 1239, 1683
SduI GDGCHC 4 cut(s) 870, 1125, 1613, 1848
SfaNI GCATC 5 cut(s) 1015, 1065, 1478, 1880, 2074
Sfr274I CTCGAG 2 cut(s) 86, 2128
SfuI TTCGAA 2 cut(s) 271, 2271
SgrAI CRCCGGYG 1 cut(s) 2675
SinI GGWCC 6 cut(s) 209, 493, 534, 654, 830, 1132
SlaI CTCGAG 2 cut(s) 86, 2128
SmiMI CAYNNNNRTG 4 cut(s) 1104, 1928, 2190, 2202
SmlI CTYRAG 3 cut(s) 86, 227, 2128
SmoI CTYRAG 3 cut(s) 86, 227, 2128
SseBI AGGCCT 3 cut(s) 1893, 1969, 2237
SsiI CCGC 7 cut(s) 418, 421, 713, 771, 1837, 2177, 2298
SspI AATATT 1 cut(s) 39
SspMI CTAG 6 cut(s) 174, 551, 981, 1536, 1787, 1889
SstI GAGCTC 3 cut(s) 870, 1613, 1848
StuI AGGCCT 3 cut(s) 1893, 1969, 2237
StyD4I CCNGG 6 cut(s) 522, 530, 1135, 1221, 1237, 1681
StyI CCWWGG 3 cut(s) 198, 252, 550
TaaI ACNGT 6 cut(s) 166, 412, 738, 1462, 2022, 2585
TaiI ACGT 4 cut(s) 642, 678, 1207, 2502
TaqI TCGA 7 cut(s) 87, 271, 444, 2129, 2257, 2271, 2343
TatI WGTACW 5 cut(s) 498, 858, 1264, 2143, 2194
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 6 cut(s) 1427, 1524, 1532, 1701, 1780, 1859
Tru1I TTAA 4 cut(s) 1422, 1493, 2121, 2692
Tru9I TTAA 4 cut(s) 1422, 1493, 2121, 2692
TscAI CASTG 7 cut(s) 171, 923, 1637, 1869, 2314, 2590, 2623
TseFI GTSAC 5 cut(s) 166, 412, 773, 1661, 2049
TseI GCWGC 8 cut(s) 624, 1184, 1625, 1649, 1868, 2062, 2252, 2396
Tsp45I GTSAC 5 cut(s) 166, 412, 773, 1661, 2049
TspDTI ATGAA 4 cut(s) 35, 361, 1364, 2590
TspGWI ACGGA 3 cut(s) 878, 1886, 2192
TspRI CASTG 7 cut(s) 171, 923, 1637, 1869, 2314, 2590, 2623
Van91I CCANNNNNTGG 1 cut(s) 1721
VpaK11BI GGWCC 6 cut(s) 209, 493, 534, 654, 830, 1132
VspI ATTAAT 1 cut(s) 1422
XapI RAATTY 6 cut(s) 267, 1390, 1693, 2074, 2381, 2545
XbaI TCTAGA 1 cut(s) 1535
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XhoI CTCGAG 2 cut(s) 86, 2128
XmaJI CCTAGG 1 cut(s) 550
XspI CTAG 6 cut(s) 174, 551, 981, 1536, 1787, 1889
ZraI GACGTC 2 cut(s) 640, 676
Zsp2I ATGCAT 1 cut(s) 2089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.