Rw5G013340

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000882
Physical Location & Seq
Reverse (-)
15662304 .. 15665808
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013340.1

Sequence Viewer

Length: 2601 bp
ATGGAAAGAAACCGATCAAAATACACACCAAAAGCCACATTCCATCATCTTCTACCATTCTTCTTCTTCTATATCTTCTTCTCTCTTGATTGCGTAGTTGCACAAGAGTTAGTTGGGTATGGATATAAAGTCGAGTCTGTGAATAATTCTGATTCCTCGGGGAAGACATTGACCGTTAACCTTGGTCTCATCAACAGCTCCTCAGTTTATGGACCTGACATATCAAATCTTACCCTCTATGTCAGCTATGAGACCAAAGACCGTCTAAGAGTTCGAATAACAGACTCAACGCAACAAAGATGGGAGCTCCCACAAGACATTCTCCCCCGCCAAAACCAAACTTCACAGAACTTTTCCGTCACTGACAACGACCTCGTTTTCACCCTCCGCAACACCACCCCGTTCGGCTTCACCATCACCCGCCGCTCCTCTAACGACACCGTTTTCGACACCACCCCAAACCCCTCCGACCCCAACACCACCTTCATCTTCAAGGACCAATACTTCCAGCTCTCCTCCTCTCTTCCCAACAACAGGTCCTCCCTGTACGGCCTCGGAGAGCACACCAAGAGCACCTTCAAGCTCCTAGCCAATCAGACTCTGACACTCTGGAGCGCCGACATCGGCAGCGTGAGTCCCGACCTCAATCTCTATGGGTCCCACCCCTTCTACATGGACGTAAGATCGCCGTCCGATGATGGCAAGGTCAAGGCGGGAACCACTCACGGAGTGCTGCTTTTGAACAGTAATGGCATGGATGTAAATTACACAGGTGACAGGGTTACTTATAATGTGATTGGCGGAGTTGTGGACTTGTATTTCTTTTCGGGTCCGACACCCGAATTGGTGATGGAGCAGTACACTGAGCTCATTGGTCGACCCGCCCCTATGCCATATTGGTCTTTCGGATTCCACCAGTGTAGATATGGTTACAAGGATGTTTCTGATCTTGAGGGGGTCGTTGCTGGTTATGCAAATGCTAGTATCCCTCTTGAGGTTATGTGGACAGACATTGATTACATGGATGCTTACAAGGATTTCACTCTTGATCCCGTCAACTTTCCATTAGACAGGATGAAGAACTTTACCAACAACCTTCACCAAAATGGTCAGAAATACGTGCTCATCTTGGATCCCGGTATTAGTATCAATGATAGCTATGGAACCTACACCAGAGGGAAGGAAGCAGATATATTTATAAAACGTGATGGCATTCCTTACCAGGGAAATGTTTGGCCTGGCAATGTTTACTTTCCTGATTTTGTAAATCCACAAAGTGAACCATATTGGGAGAATGAGATTAAACTATTTCTAGACCAGCTACCTGTTGATGGCCTTTGGCTTGATATGAATGAAGTATCAAATTTCCAAACTTCTAATCCCACTCCGAATTCCACACTTGATGATCCACCTTACAAAATCAACGATGCTGGAGGCCACCGTCCGATCCTTAGTAGGACTATTCCAGGATCAGCTCTACACTTTGGTAATGTTACAGAGTATAATGTTCATAACCTATTTGGCATGTTAGAAGCCAAAGCAACAAACAAGGCCTTGACCAATTTGACTGGTAAGAGACCTTTTGTTCTCTCGAGATCGACTTTTGTAAGCTCTGGTAAGTACACAGCACACTGGACCGGAGACAATGGTGCAAAATGGATTGATTTGGCATACTCAATTCCGGGCATTTTGAATTTTGGACTCTTTGGAGTTCCAATGGTTGGAGCTGATATATGTGGTTTCTCTGGGGATACTAATGAAGAACTCTGCCGGCGTTGGATTCAGCTAGGGGCCTTTTACCCCTTTTCAAGAGATCATTCGGATAAGAATTCAATCCGGCAGGAGCTCTATGTTTGGGAATCAGTGGCTGCATCAGCAAAAAAAGTGCTGGGGCTACGTTACCGGTTGCTTCCCTTGTTCTATACATCAATGTATCAAGCACACAAGAACGGGACTCCAATTGCAAGGCCCGTATTCTTCTCATTTCCTGAAGATACCAATACATACAGTATCTATTCACAGTTTCTGATTGGTAAAGGCGTAATGGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTGAGGCATATTTTCCTGCAGGAAATTGGTTCGATCTCTTCAACTACACATCAGTTAGTGTAGACTCTGGAAAGAATGTTACGTTGGATGCACCGCCTGATCATATCAATGTGCATGTCCACGAAGGAAACATTTTGGCCTTGCAAGGAGAGGCCTTGACAACACTAGCAGCCCGAAACACTACATTCGAACTTTTGGTGGTCACTGGTAGTAGCAATGAGAGTACCGGAGAATTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTTGTGAGGTTTCGTTCTGCACGAGCACAAAATGGAAGTCTAGTTTTGAGCTCAAAGGTCGAAAATGGAGAATATGCATTAAGCCAAGGTTGGATCATTGATAAAATTACCGTCCTTGGGTTGGACAAAGTTAATGCAACACAGTCCAGTGTATCCCTTCCTATAGGAAAGGAGTTTGAGTTGGAGCTGAAGCCGAATCAGACACTGGCGAACCCAAAAACATATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

866

Amino Acids

96.84

Weight (kDa)

5.2

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 82 - 250 2.6e-10 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 294 - 642 4.1e-122 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 651 - 739 2.9e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 789, 1199
AccB7I CCANNNNNTGG 1 cut(s) 1721
AccBSI CCGCTC 1 cut(s) 426
AccI GTMKAC 2 cut(s) 877, 2142
AciI CCGC 8 cut(s) 328, 388, 421, 424, 713, 801, 882, 2174
AclWI GGATC 7 cut(s) 1043, 1127, 1140, 1400, 1441, 1477, 2471
AcsI RAATTY 5 cut(s) 1363, 1390, 1693, 1828, 2074
AcuI CTGAAG 2 cut(s) 2010, 2579
AdeI CACNNNGTG 2 cut(s) 730, 1277
AfaI GTAC 4 cut(s) 548, 860, 1622, 2305
AfiI CCNNNNNNNGG 7 cut(s) 534, 994, 1223, 1331, 1721, 2487, 2491
AgeI ACCGGT 1 cut(s) 1902
AgsI TTSAA 7 cut(s) 493, 580, 742, 1693, 1809, 1833, 2122
AjnI CCWGG 3 cut(s) 1221, 1237, 1465
AjuI GAANNNNNNNTTGG 6 cut(s) 467, 499, 560, 592, 2147, 2179
Alw21I GWGCWC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
Alw26I GTCTC 4 cut(s) 191, 245, 1570, 1635
AlwI GGATC 7 cut(s) 1043, 1127, 1140, 1400, 1441, 1477, 2471
AlwNI CAGNNNCTG 4 cut(s) 601, 1868, 2026, 2575
Ama87I CYCGRG 2 cut(s) 157, 1591
AoxI GGCC 9 cut(s) 550, 1235, 1333, 1435, 1551, 1791, 1967, 2217, 2232
ApeKI GCWGC 5 cut(s) 627, 733, 1868, 2062, 2249
ApoI RAATTY 5 cut(s) 1363, 1390, 1693, 1828, 2074
ArsI GACNNNNNNTTYG 4 cut(s) 428, 460, 2392, 2424
AsiGI ACCGGT 1 cut(s) 1902
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 8 cut(s) 212, 496, 537, 657, 830, 1635, 1791, 1968
AsuC2I CCSGG 2 cut(s) 1137, 1683
AsuHPI GGTGA 7 cut(s) 373, 403, 409, 785, 859, 1091, 2043
AsuII TTCGAA 2 cut(s) 274, 2268
AvaI CYCGRG 2 cut(s) 157, 1591
AvaII GGWCC 6 cut(s) 212, 496, 537, 657, 830, 1635
BamHI GGATCC 1 cut(s) 1132
BanII GRGCYC 4 cut(s) 309, 870, 1848, 2423
BauI CACGAG 1 cut(s) 2391
BbsI GAAGAC 1 cut(s) 170
Bbv12I GWGCWC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
BbvI GCAGC 5 cut(s) 639, 720, 1855, 2074, 2261
BccI CCATC 8 cut(s) 51, 294, 422, 692, 844, 1202, 1325, 2321
BceAI ACGGC 2 cut(s) 565, 673
BciT130I CCWGG 3 cut(s) 1223, 1239, 1467
BciVI GTATCC 3 cut(s) 995, 1744, 2533
BclI TGATCA 1 cut(s) 2179
BcnI CCSGG 2 cut(s) 1137, 1683
BcoDI GTCTC 4 cut(s) 191, 245, 1570, 1635
BfaI CTAG 6 cut(s) 587, 981, 1313, 1787, 2246, 2411
BfmI CTRYAG 2 cut(s) 2097, 2532
BfoI RGCGCY 1 cut(s) 618
BfuI GTATCC 3 cut(s) 995, 1744, 2533
BisI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
BlsI GCNGC 6 cut(s) 425, 629, 735, 1870, 2064, 2251
Bme1390I CCNGG 5 cut(s) 1137, 1223, 1239, 1467, 1683
Bme18I GGWCC 6 cut(s) 212, 496, 537, 657, 830, 1635
BmeT110I CYCGRG 2 cut(s) 157, 1591
BmgT120I GGNCC 8 cut(s) 212, 496, 537, 657, 830, 1635, 1791, 1968
BmiI GGNNCC 7 cut(s) 658, 659, 718, 831, 1134, 1165, 1792
BmrFI CCNGG 5 cut(s) 1137, 1223, 1239, 1467, 1683
BmsI GCATC 4 cut(s) 1015, 1417, 1880, 2158
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 2 cut(s) 631, 1452
Bpu14I TTCGAA 2 cut(s) 274, 2268
BpuEI CTTGAG 2 cut(s) 971, 1013
BpuMI CCSGG 2 cut(s) 1137, 1683
BsaAI YACGTR 1 cut(s) 1120
BsaI GGTCTC 3 cut(s) 191, 245, 1570
BsaJI CCNNGG 6 cut(s) 156, 181, 553, 1222, 2455, 2485
BsaWI WCCGGW 3 cut(s) 1637, 1902, 2306
BsaXI ACNNNNNCTCC 6 cut(s) 524, 554, 2322, 2352, 2534, 2564
Bsc4I CCNNNNNNNGG 7 cut(s) 534, 994, 1223, 1331, 1721, 2487, 2491
Bse118I RCCGGY 2 cut(s) 1770, 1902
Bse1I ACTGG 6 cut(s) 916, 1573, 1637, 2290, 2517, 2580
Bse3DI GCAATG 2 cut(s) 1249, 2302
BseBI CCWGG 3 cut(s) 1223, 1239, 1467
BseDI CCNNGG 6 cut(s) 156, 181, 553, 1222, 2455, 2485
BseGI GGATG 6 cut(s) 763, 943, 1030, 1080, 2173, 2329
BseLI CCNNNNNNNGG 7 cut(s) 534, 994, 1223, 1331, 1721, 2487, 2491
BseMI GCAATG 2 cut(s) 1249, 2302
BseMII CTCAG 2 cut(s) 216, 855
BseNI ACTGG 6 cut(s) 916, 1573, 1637, 2290, 2517, 2580
BseRI GAGGAG 5 cut(s) 190, 418, 505, 508, 2364
BseXI GCAGC 5 cut(s) 639, 720, 1855, 2074, 2261
BseYI CCCAGC 1 cut(s) 1888
BsgI GTGCAG 1 cut(s) 2373
BshFI GGCC 9 cut(s) 552, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
BshTI ACCGGT 1 cut(s) 1902
BsiHKAI GWGCWC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
BsiHKCI CYCGRG 2 cut(s) 157, 1591
BsiSI CCGG 7 cut(s) 1137, 1638, 1682, 1771, 1837, 1903, 2307
BslFI GGGAC 3 cut(s) 621, 643, 1966
BslI CCNNNNNNNGG 7 cut(s) 534, 994, 1223, 1331, 1721, 2487, 2491
BsmAI GTCTC 4 cut(s) 191, 245, 1570, 1635
BsmFI GGGAC 3 cut(s) 621, 643, 1966
BsmI GAATGC 1 cut(s) 1212
BsnI GGCC 9 cut(s) 552, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
Bso31I GGTCTC 3 cut(s) 191, 245, 1570
BsoBI CYCGRG 2 cut(s) 157, 1591
Bsp119I TTCGAA 2 cut(s) 274, 2268
Bsp1286I GDGCHC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
BspACI CCGC 8 cut(s) 328, 388, 421, 424, 713, 801, 882, 2174
BspANI GGCC 9 cut(s) 552, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
BspCNI CTCAG 2 cut(s) 215, 856
BspLI GGNNCC 7 cut(s) 658, 659, 718, 831, 1134, 1165, 1792
BspMAI CTGCAG 1 cut(s) 2101
BspPI GGATC 7 cut(s) 1043, 1127, 1140, 1400, 1441, 1477, 2471
BspT104I TTCGAA 2 cut(s) 274, 2268
BspTNI GGTCTC 3 cut(s) 191, 245, 1570
BsrBI CCGCTC 1 cut(s) 426
BsrDI GCAATG 2 cut(s) 1249, 2302
BsrFI RCCGGY 2 cut(s) 1770, 1902
BsrI ACTGG 6 cut(s) 916, 1573, 1637, 2290, 2517, 2580
BssAI RCCGGY 2 cut(s) 1770, 1902
BssECI CCNNGG 6 cut(s) 156, 181, 553, 1222, 2455, 2485
BssSI CACGAG 1 cut(s) 2391
BssT1I CCWWGG 3 cut(s) 181, 2455, 2485
Bst2BI CACGAG 1 cut(s) 2391
Bst2UI CCWGG 3 cut(s) 1223, 1239, 1467
Bst4CI ACNGT 9 cut(s) 175, 263, 442, 746, 1442, 2009, 2022, 2482, 2514
Bst6I CTCTTC 2 cut(s) 528, 2123
BstBAI YACGTR 1 cut(s) 1120
BstBI TTCGAA 2 cut(s) 274, 2268
BstC8I GCNNGC 1 cut(s) 1772
BstDEI CTNAG 4 cut(s) 202, 266, 864, 1451
BstF5I GGATG 6 cut(s) 763, 943, 1030, 1080, 2173, 2329
BstH2I RGCGCY 1 cut(s) 618
BstHHI GCGC 1 cut(s) 617
BstMAI GTCTC 4 cut(s) 191, 245, 1570, 1635
BstMWI GCNNNNNNNGC 2 cut(s) 971, 1874
BstNI CCWGG 3 cut(s) 1223, 1239, 1467
BstNSI RCATGY 2 cut(s) 1528, 2198
BstSCI CCNGG 5 cut(s) 1135, 1221, 1237, 1465, 1681
BstSFI CTRYAG 2 cut(s) 2097, 2532
BstV1I GCAGC 5 cut(s) 639, 720, 1855, 2074, 2261
BstV2I GAAGAC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 1132
BstYI RGATCY 1 cut(s) 1132
BsuI GTATCC 3 cut(s) 995, 1744, 2533
BsuRI GGCC 9 cut(s) 552, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
BtsCI GGATG 6 cut(s) 763, 943, 1030, 1080, 2173, 2329
BtsIMutI CAGTG 8 cut(s) 360, 861, 923, 1630, 1869, 2283, 2524, 2573
Cac8I GCNNGC 1 cut(s) 1772
CaiI CAGNNNCTG 4 cut(s) 601, 1868, 2026, 2575
CfoI GCGC 1 cut(s) 617
Cfr10I RCCGGY 2 cut(s) 1770, 1902
Cfr13I GGNCC 8 cut(s) 212, 496, 537, 657, 830, 1635, 1791, 1968
Csp6I GTAC 4 cut(s) 547, 859, 1621, 2304
CspAI ACCGGT 1 cut(s) 1902
CviAII CATG 5 cut(s) 673, 754, 1021, 1525, 2195
CviQI GTAC 4 cut(s) 547, 859, 1621, 2304
DdeI CTNAG 4 cut(s) 202, 266, 864, 1451
DraIII CACNNNGTG 2 cut(s) 730, 1277
Eam1104I CTCTTC 2 cut(s) 528, 2123
EarI CTCTTC 2 cut(s) 528, 2123
EciI GGCGGA 1 cut(s) 816
Ecl136II GAGCTC 4 cut(s) 307, 868, 1846, 2421
Eco130I CCWWGG 3 cut(s) 181, 2455, 2485
Eco147I AGGCCT 2 cut(s) 1553, 2234
Eco24I GRGCYC 4 cut(s) 309, 870, 1848, 2423
Eco31I GGTCTC 3 cut(s) 191, 245, 1570
Eco47I GGWCC 6 cut(s) 212, 496, 537, 657, 830, 1635
Eco53kI GAGCTC 4 cut(s) 307, 868, 1846, 2421
Eco57I CTGAAG 2 cut(s) 2010, 2579
Eco88I CYCGRG 2 cut(s) 157, 1591
EcoICRI GAGCTC 4 cut(s) 307, 868, 1846, 2421
EcoO109I RGGNCCY 3 cut(s) 537, 657, 1791
EcoRI GAATTC 2 cut(s) 1390, 1828
EcoRII CCWGG 3 cut(s) 1221, 1237, 1465
EcoT14I CCWWGG 3 cut(s) 181, 2455, 2485
EcoT22I ATGCAT 1 cut(s) 2449
EcoT38I GRGCYC 4 cut(s) 309, 870, 1848, 2423
ErhI CCWWGG 3 cut(s) 181, 2455, 2485
FaeI CATG 5 cut(s) 676, 757, 1024, 1528, 2198
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 3 cut(s) 621, 643, 1966
FatI CATG 5 cut(s) 672, 753, 1020, 1524, 2194
FauI CCCGC 4 cut(s) 335, 428, 706, 889
FbaI TGATCA 1 cut(s) 2179
FblI GTMKAC 2 cut(s) 877, 2142
Fnu4HI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
FokI GGATG 6 cut(s) 770, 950, 1037, 1087, 2180, 2336
FriOI GRGCYC 4 cut(s) 309, 870, 1848, 2423
Fsp4HI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
FspBI CTAG 6 cut(s) 587, 981, 1313, 1787, 2246, 2411
GlaI GCGC 1 cut(s) 616
GluI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
GsaI CCCAGC 1 cut(s) 1892
GsuI CTGGAG 2 cut(s) 631, 1452
HaeII RGCGCY 1 cut(s) 618
HaeIII GGCC 9 cut(s) 552, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
HapII CCGG 7 cut(s) 1137, 1638, 1682, 1771, 1837, 1903, 2307
HhaI GCGC 1 cut(s) 617
Hin1II CATG 5 cut(s) 676, 757, 1024, 1528, 2198
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HincII GTYRAC 3 cut(s) 178, 878, 1057
HindII GTYRAC 3 cut(s) 178, 878, 1057
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 7 cut(s) 1137, 1638, 1682, 1771, 1837, 1903, 2307
HphI GGTGA 7 cut(s) 373, 403, 409, 785, 859, 1091, 2043
HpyAV CCTTC 7 cut(s) 493, 586, 676, 1106, 1174, 2198, 2537
HpyCH4III ACNGT 9 cut(s) 175, 263, 442, 746, 1442, 2009, 2022, 2482, 2514
HpyCH4IV ACGT 5 cut(s) 678, 1119, 1204, 1897, 2162
HpyF10VI GCNNNNNNNGC 2 cut(s) 971, 1874
HpyF3I CTNAG 4 cut(s) 202, 266, 864, 1451
HpySE526I ACGT 5 cut(s) 678, 1119, 1204, 1897, 2162
Hsp92II CATG 5 cut(s) 676, 757, 1024, 1528, 2198
HspAI GCGC 1 cut(s) 615
KflI GGGWCCC 1 cut(s) 657
KroI GCCGGC 1 cut(s) 1770
KroNI GCCGGC 1 cut(s) 1772
Ksp22I TGATCA 1 cut(s) 2179
KspAI GTTAAC 1 cut(s) 178
Lsp1109I GCAGC 5 cut(s) 639, 720, 1855, 2074, 2261
LweI GCATC 4 cut(s) 1015, 1417, 1880, 2158
MaeI CTAG 6 cut(s) 587, 981, 1313, 1787, 2246, 2411
MaeII ACGT 5 cut(s) 678, 1119, 1204, 1897, 2162
MaeIII GTNAC 9 cut(s) 358, 773, 781, 929, 1492, 1898, 2049, 2158, 2281
MbiI CCGCTC 1 cut(s) 426
MfeI CAATTG 1 cut(s) 1959
MflI RGATCY 1 cut(s) 1132
MhlI GDGCHC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
MlyI GAGTC 7 cut(s) 143, 278, 592, 643, 1695, 1948, 2138
MmeI TCCRAC 8 cut(s) 492, 857, 1702, 1757, 2145, 2441, 2472, 2532
Mph1103I ATGCAT 1 cut(s) 2449
MroNI GCCGGC 1 cut(s) 1770
MseI TTAA 4 cut(s) 177, 1302, 2450, 2502
MslI CAYNNNNRTG 3 cut(s) 1104, 2187, 2596
MspI CCGG 7 cut(s) 1137, 1638, 1682, 1771, 1837, 1903, 2307
MspR9I CCNGG 5 cut(s) 1137, 1223, 1239, 1467, 1683
MunI CAATTG 1 cut(s) 1959
Mva1269I GAATGC 1 cut(s) 1212
MvaI CCWGG 3 cut(s) 1223, 1239, 1467
MwoI GCNNNNNNNGC 2 cut(s) 971, 1874
NaeI GCCGGC 1 cut(s) 1772
NciI CCSGG 2 cut(s) 1137, 1683
NgoMIV GCCGGC 1 cut(s) 1770
NlaIII CATG 5 cut(s) 676, 757, 1024, 1528, 2198
NlaIV GGNNCC 7 cut(s) 658, 659, 718, 831, 1134, 1165, 1792
NmuCI GTSAC 4 cut(s) 358, 773, 2049, 2281
NsiI ATGCAT 1 cut(s) 2449
NspI RCATGY 2 cut(s) 1528, 2198
NspV TTCGAA 2 cut(s) 274, 2268
PaeR7I CTCGAG 1 cut(s) 1591
PceI AGGCCT 2 cut(s) 1553, 2234
PcsI WCGNNNNNNNCGW 1 cut(s) 2389
PctI GAATGC 1 cut(s) 1212
PdiI GCCGGC 1 cut(s) 1772
PfeI GAWTC 5 cut(s) 152, 909, 1780, 1859, 2566
PflMI CCANNNNNTGG 1 cut(s) 1721
PfoI TCCNGGA 1 cut(s) 1465
PinAI ACCGGT 1 cut(s) 1902
PkrI GCNGC 6 cut(s) 425, 629, 735, 1870, 2064, 2251
PleI GAGTC 7 cut(s) 142, 278, 592, 642, 1695, 1948, 2138
PpsI GAGTC 7 cut(s) 142, 278, 592, 642, 1695, 1948, 2138
Ppu21I YACGTR 1 cut(s) 1120
PpuMI RGGWCCY 2 cut(s) 537, 657
PsiI TTATAA 2 cut(s) 789, 1199
Psp124BI GAGCTC 4 cut(s) 309, 870, 1848, 2423
Psp5II RGGWCCY 2 cut(s) 537, 657
Psp6I CCWGG 3 cut(s) 1221, 1237, 1465
PspFI CCCAGC 1 cut(s) 1888
PspGI CCWGG 3 cut(s) 1221, 1237, 1465
PspN4I GGNNCC 7 cut(s) 658, 659, 718, 831, 1134, 1165, 1792
PspPI GGNCC 8 cut(s) 212, 496, 537, 657, 830, 1635, 1791, 1968
PspPPI RGGWCCY 2 cut(s) 537, 657
PstI CTGCAG 1 cut(s) 2101
PstNI CAGNNNCTG 4 cut(s) 601, 1868, 2026, 2575
PsuI RGATCY 1 cut(s) 1132
RsaI GTAC 4 cut(s) 548, 860, 1622, 2305
RsaNI GTAC 4 cut(s) 547, 859, 1621, 2304
RseI CAYNNNNRTG 3 cut(s) 1104, 2187, 2596
SacI GAGCTC 4 cut(s) 309, 870, 1848, 2423
SalI GTCGAC 1 cut(s) 876
SaqAI TTAA 4 cut(s) 177, 1302, 2450, 2502
SatI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
Sau96I GGNCC 8 cut(s) 212, 496, 537, 657, 830, 1635, 1791, 1968
SbfI CCTGCAGG 1 cut(s) 2101
SchI GAGTC 7 cut(s) 143, 278, 592, 643, 1695, 1948, 2138
ScrFI CCNGG 5 cut(s) 1137, 1223, 1239, 1467, 1683
SdaI CCTGCAGG 1 cut(s) 2101
SduI GDGCHC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
SfaNI GCATC 4 cut(s) 1015, 1417, 1880, 2158
SfcI CTRYAG 2 cut(s) 2097, 2532
Sfr274I CTCGAG 1 cut(s) 1591
SfuI TTCGAA 2 cut(s) 274, 2268
SinI GGWCC 6 cut(s) 212, 496, 537, 657, 830, 1635
SlaI CTCGAG 1 cut(s) 1591
SmiMI CAYNNNNRTG 3 cut(s) 1104, 2187, 2596
SmlI CTYRAG 3 cut(s) 950, 992, 1591
SmoI CTYRAG 3 cut(s) 950, 992, 1591
Sse8387I CCTGCAGG 1 cut(s) 2101
SseBI AGGCCT 2 cut(s) 1553, 2234
SsiI CCGC 8 cut(s) 328, 388, 421, 424, 713, 801, 882, 2174
SspMI CTAG 6 cut(s) 587, 981, 1313, 1787, 2246, 2411
SstI GAGCTC 4 cut(s) 309, 870, 1848, 2423
StuI AGGCCT 2 cut(s) 1553, 2234
StyD4I CCNGG 5 cut(s) 1135, 1221, 1237, 1465, 1681
StyI CCWWGG 3 cut(s) 181, 2455, 2485
TaaI ACNGT 9 cut(s) 175, 263, 442, 746, 1442, 2009, 2022, 2482, 2514
TaiI ACGT 5 cut(s) 681, 1122, 1207, 1900, 2165
TatI WGTACW 2 cut(s) 858, 1620
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 5 cut(s) 152, 909, 1780, 1859, 2566
Tru1I TTAA 4 cut(s) 177, 1302, 2450, 2502
Tru9I TTAA 4 cut(s) 177, 1302, 2450, 2502
TscAI CASTG 8 cut(s) 367, 868, 923, 1637, 1869, 2290, 2524, 2580
TseFI GTSAC 4 cut(s) 358, 773, 2049, 2281
TseI GCWGC 5 cut(s) 627, 733, 1868, 2062, 2249
Tsp45I GTSAC 4 cut(s) 358, 773, 2049, 2281
TspDTI ATGAA 6 cut(s) 475, 1091, 1364, 1368, 1499, 1773
TspGWI ACGGA 2 cut(s) 346, 741
TspRI CASTG 8 cut(s) 367, 868, 923, 1637, 1869, 2290, 2524, 2580
Van91I CCANNNNNTGG 1 cut(s) 1721
VpaK11BI GGWCC 6 cut(s) 212, 496, 537, 657, 830, 1635
XapI RAATTY 5 cut(s) 1363, 1390, 1693, 1828, 2074
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 2 cut(s) 1528, 2198
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XhoI CTCGAG 1 cut(s) 1591
XmiI GTMKAC 2 cut(s) 877, 2142
XspI CTAG 6 cut(s) 587, 981, 1313, 1787, 2246, 2411
Zsp2I ATGCAT 1 cut(s) 2449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.