Rorug05G0045400

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000882
Physical Location & Seq
Forward (+)
3960372 .. 3961726
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0045400.1

Sequence Viewer

Length: 753 bp
ATGTTTAAATCCTGGTTCCGCCACTGGCAGGGGAAGACAAAATTTTTTAGTGTGTTGATGCTCGCATGGGGCTTAGTAGCAGATGTTGACATTGAGTCTGAAAAGTATAGGTGGATGGGAAGTGCTCGTCTAGATTTCTATGCCATTCAACGAATTGTGCATTTGAGGCAATATAACGGATCTATTTCTTTTGTGCCTGCACCTGGCTTCGAAGGGTTTGGAGAGCCTACTAGTTACAATGACGATGATGAATCTACTAACACTGATCCAAGTCAAGAAAAGCCTACTAAGGTTCGACAACTCGGCTATGAGGGACCTGATATTAATCTAAAGAACATGAACTGGAGAACAATCAAAGGACCGTTTGTTTCAGTGTGGCTTCACAATGTACCTTGGGGAGGTGAAGACACAAGGGCAGCTCCTGATGCCAAGTTCTCAGATGGTTATCTAGACGTTATAATTATGAGGGCTTGCCCAAAATTGTCCTTGCTCTCGTTAATGACGGGGTTGAGCACTGGGACTCATGTCAAGTCACCATATGTGCTATACTTCAAGGTGAAAGCTTCCATTCTAGAGCCTGGTGCTCTCGCTGATGATTCAACAAAGGAAGGGATAGTGGACTCAGATGGTGAGGTCCTTGCTAGGGGCAAAGGAGCATACAAATGTCATCAGAAAACACTAATGGTGTACGATAAACTTCAAATAACTTTGGATCAAGGTTTAGCCACTCTGTTTTCCCCTTCCCCGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

250

Amino Acids

28.03

Weight (kDa)

6.52

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YegS_C PF19279 123 - 227 2.6e-12 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 458
AciI CCGC 1 cut(s) 19
AclWI GGATC 3 cut(s) 187, 260, 720
AcsI RAATTY 1 cut(s) 41
AfaI GTAC 2 cut(s) 390, 689
AfiI CCNNNNNNNGG 5 cut(s) 28, 203, 398, 643, 745
AgsI TTSAA 4 cut(s) 149, 553, 600, 701
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 230
AjnI CCWGG 3 cut(s) 11, 202, 577
AluBI AGCT 2 cut(s) 419, 563
AluI AGCT 2 cut(s) 419, 563
Alw21I GWGCWC 3 cut(s) 127, 515, 586
AlwI GGATC 3 cut(s) 187, 260, 720
AlwNI CAGNNNCTG 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 416
ApoI RAATTY 1 cut(s) 41
AseI ATTAAT 1 cut(s) 324
AspS9I GGNCC 3 cut(s) 314, 359, 634
AsuC2I CCSGG 1 cut(s) 746
AsuHPI GGTGA 4 cut(s) 413, 525, 568, 641
AsuII TTCGAA 1 cut(s) 210
AvaII GGWCC 3 cut(s) 314, 359, 634
BbsI GAAGAC 2 cut(s) 41, 411
Bbv12I GWGCWC 3 cut(s) 127, 515, 586
BbvI GCAGC 1 cut(s) 428
BccI CCATC 3 cut(s) 109, 434, 620
BciT130I CCWGG 3 cut(s) 13, 204, 579
BcnI CCSGG 1 cut(s) 746
BcuI ACTAGT 1 cut(s) 230
BfaI CTAG 5 cut(s) 131, 231, 449, 572, 642
BisI GCNGC 1 cut(s) 417
BlsI GCNGC 1 cut(s) 418
Bme1390I CCNGG 4 cut(s) 13, 204, 579, 746
Bme18I GGWCC 3 cut(s) 314, 359, 634
BmeRI GACNNNNNGTC 1 cut(s) 94
BmgT120I GGNCC 3 cut(s) 314, 359, 634
BmiI GGNNCC 2 cut(s) 17, 315
BmrFI CCNGG 4 cut(s) 13, 204, 579, 746
BmrI ACTGGG 1 cut(s) 525
BmsI GCATC 2 cut(s) 48, 415
BmuI ACTGGG 1 cut(s) 525
BoxI GACNNNNGTC 1 cut(s) 524
BpiI GAAGAC 2 cut(s) 41, 411
BpmI CTGGAG 1 cut(s) 364
Bpu14I TTCGAA 1 cut(s) 210
BpuMI CCSGG 1 cut(s) 746
BsaBI GATNNNNATC 2 cut(s) 324, 444
BsaJI CCNNGG 2 cut(s) 392, 744
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 203, 398, 643, 745
Bse1I ACTGG 3 cut(s) 29, 347, 520
Bse8I GATNNNNATC 2 cut(s) 324, 444
BseBI CCWGG 3 cut(s) 13, 204, 579
BseDI CCNNGG 2 cut(s) 392, 744
BseGI GGATG 1 cut(s) 120
BseJI GATNNNNATC 2 cut(s) 324, 444
BseLI CCNNNNNNNGG 5 cut(s) 28, 203, 398, 643, 745
BseMII CTCAG 2 cut(s) 450, 636
BseNI ACTGG 3 cut(s) 29, 347, 520
BseXI GCAGC 1 cut(s) 428
BsgI GTGCAG 1 cut(s) 183
BsiHKAI GWGCWC 3 cut(s) 127, 515, 586
BsiSI CCGG 1 cut(s) 746
BslFI GGGAC 2 cut(s) 327, 532
BslI CCNNNNNNNGG 5 cut(s) 28, 203, 398, 643, 745
BsmFI GGGAC 2 cut(s) 327, 532
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 3 cut(s) 127, 515, 586
Bsp143I GATC 3 cut(s) 179, 265, 712
BspACI CCGC 1 cut(s) 19
BspCNI CTCAG 2 cut(s) 449, 635
BspLI GGNNCC 2 cut(s) 17, 315
BspPI GGATC 3 cut(s) 187, 260, 720
BspT104I TTCGAA 1 cut(s) 210
BsrI ACTGG 3 cut(s) 29, 347, 520
BssECI CCNNGG 2 cut(s) 392, 744
BssMI GATC 3 cut(s) 179, 265, 712
BssT1I CCWWGG 1 cut(s) 392
Bst2UI CCWGG 3 cut(s) 13, 204, 579
Bst4CI ACNGT 1 cut(s) 363
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 63, 198, 472
BstDEI CTNAG 4 cut(s) 73, 288, 436, 622
BstENI CCTNNNNNAGG 1 cut(s) 396
BstF5I GGATG 1 cut(s) 120
BstKTI GATC 3 cut(s) 182, 268, 715
BstMBI GATC 3 cut(s) 179, 265, 712
BstMWI GCNNNNNNNGC 2 cut(s) 166, 425
BstNI CCWGG 3 cut(s) 13, 204, 579
BstPAI GACNNNNGTC 1 cut(s) 524
BstSCI CCNGG 4 cut(s) 11, 202, 577, 744
BstV1I GCAGC 1 cut(s) 428
BstV2I GAAGAC 2 cut(s) 41, 411
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BtsCI GGATG 1 cut(s) 120
BtsIMutI CAGTG 4 cut(s) 22, 261, 378, 513
Cac8I GCNNGC 3 cut(s) 63, 198, 472
CaiI CAGNNNCTG 1 cut(s) 422
Cfr13I GGNCC 3 cut(s) 314, 359, 634
Csp6I GTAC 2 cut(s) 389, 688
CviAII CATG 3 cut(s) 66, 337, 524
CviQI GTAC 2 cut(s) 389, 688
DdeI CTNAG 4 cut(s) 73, 288, 436, 622
DpnI GATC 3 cut(s) 181, 267, 714
DpnII GATC 3 cut(s) 179, 265, 712
DraI TTTAAA 1 cut(s) 7
DriI GACNNNNNGTC 1 cut(s) 94
Eam1105I GACNNNNNGTC 1 cut(s) 94
EciI GGCGGA 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 392
Eco47I GGWCC 3 cut(s) 314, 359, 634
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 314, 634
EcoRII CCWGG 3 cut(s) 11, 202, 577
EcoT14I CCWWGG 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 392
FaeI CATG 3 cut(s) 69, 340, 527
FaqI GGGAC 2 cut(s) 327, 532
FatI CATG 3 cut(s) 65, 336, 523
FauNDI CATATG 1 cut(s) 538
Fnu4HI GCNGC 1 cut(s) 417
FokI GGATG 1 cut(s) 127
Fsp4HI GCNGC 1 cut(s) 417
FspBI CTAG 5 cut(s) 131, 231, 449, 572, 642
GluI GCNGC 1 cut(s) 417
GsuI CTGGAG 1 cut(s) 364
HapII CCGG 1 cut(s) 746
Hin1II CATG 3 cut(s) 69, 340, 527
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 561
HinfI GANTC 5 cut(s) 95, 251, 520, 596, 620
HpaII CCGG 1 cut(s) 746
HphI GGTGA 4 cut(s) 413, 525, 568, 641
Hpy166II GTNNAC 3 cut(s) 88, 619, 688
Hpy188I TCNGA 4 cut(s) 100, 439, 625, 672
Hpy188III TCNNGA 5 cut(s) 131, 275, 422, 449, 572
Hpy8I GTNNAC 3 cut(s) 88, 619, 688
HpyAV CCTTC 3 cut(s) 206, 602, 750
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 2 cut(s) 160, 200
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 425
HpyF3I CTNAG 4 cut(s) 73, 288, 436, 622
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 3 cut(s) 69, 340, 527
Kzo9I GATC 3 cut(s) 179, 265, 712
LmnI GCTCC 2 cut(s) 424, 653
Lsp1109I GCAGC 1 cut(s) 428
LweI GCATC 2 cut(s) 48, 415
MaeI CTAG 5 cut(s) 131, 231, 449, 572, 642
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 2 cut(s) 233, 531
MalI GATC 3 cut(s) 181, 267, 714
MboI GATC 3 cut(s) 179, 265, 712
MboII GAAGA 2 cut(s) 46, 416
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 3 cut(s) 127, 515, 586
MluCI AATT 4 cut(s) 41, 153, 459, 479
MlyI GAGTC 3 cut(s) 104, 514, 614
MnlI CCTC 5 cut(s) 159, 304, 392, 459, 625
MseI TTAA 3 cut(s) 6, 324, 497
MslI CAYNNNNRTG 1 cut(s) 661
MspI CCGG 1 cut(s) 746
MspR9I CCNGG 4 cut(s) 13, 204, 579, 746
MvaI CCWGG 3 cut(s) 13, 204, 579
MwoI GCNNNNNNNGC 2 cut(s) 166, 425
NciI CCSGG 1 cut(s) 746
NdeI CATATG 1 cut(s) 538
NdeII GATC 3 cut(s) 179, 265, 712
NlaIII CATG 3 cut(s) 69, 340, 527
NlaIV GGNNCC 2 cut(s) 17, 315
NmeAIII GCCGAG 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 531
NspV TTCGAA 1 cut(s) 210
PfeI GAWTC 2 cut(s) 251, 596
PkrI GCNGC 1 cut(s) 418
PleI GAGTC 3 cut(s) 103, 514, 614
PpsI GAGTC 3 cut(s) 103, 514, 614
PpuMI RGGWCCY 2 cut(s) 314, 634
PshAI GACNNNNGTC 1 cut(s) 524
PshBI ATTAAT 1 cut(s) 324
PsiI TTATAA 1 cut(s) 458
Psp5II RGGWCCY 2 cut(s) 314, 634
Psp6I CCWGG 3 cut(s) 11, 202, 577
PspGI CCWGG 3 cut(s) 11, 202, 577
PspN4I GGNNCC 2 cut(s) 17, 315
PspPI GGNCC 3 cut(s) 314, 359, 634
PspPPI RGGWCCY 2 cut(s) 314, 634
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 179
RsaI GTAC 2 cut(s) 390, 689
RsaNI GTAC 2 cut(s) 389, 688
RseI CAYNNNNRTG 1 cut(s) 661
SaqAI TTAA 3 cut(s) 6, 324, 497
SatI GCNGC 1 cut(s) 417
Sau3AI GATC 3 cut(s) 179, 265, 712
Sau96I GGNCC 3 cut(s) 314, 359, 634
SchI GAGTC 3 cut(s) 104, 514, 614
ScrFI CCNGG 4 cut(s) 13, 204, 579, 746
SduI GDGCHC 3 cut(s) 127, 515, 586
SfaNI GCATC 2 cut(s) 48, 415
SfuI TTCGAA 1 cut(s) 210
SinI GGWCC 3 cut(s) 314, 359, 634
SmiMI CAYNNNNRTG 1 cut(s) 661
SpeI ACTAGT 1 cut(s) 230
Sse9I AATT 4 cut(s) 41, 153, 459, 479
SsiI CCGC 1 cut(s) 19
SspMI CTAG 5 cut(s) 131, 231, 449, 572, 642
StyD4I CCNGG 4 cut(s) 11, 202, 577, 744
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 1 cut(s) 363
TaiI ACGT 1 cut(s) 456
TaqI TCGA 2 cut(s) 210, 295
TasI AATT 4 cut(s) 41, 153, 459, 479
TfiI GAWTC 2 cut(s) 251, 596
Tru1I TTAA 3 cut(s) 6, 324, 497
Tru9I TTAA 3 cut(s) 6, 324, 497
TscAI CASTG 4 cut(s) 29, 268, 378, 520
TseFI GTSAC 1 cut(s) 531
TseI GCWGC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 531
TspDTI ATGAA 2 cut(s) 264, 353
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 4 cut(s) 29, 268, 378, 520
VpaK11BI GGWCC 3 cut(s) 314, 359, 634
VspI ATTAAT 1 cut(s) 324
XagI CCTNNNNNAGG 1 cut(s) 396
XapI RAATTY 1 cut(s) 41
XbaI TCTAGA 3 cut(s) 130, 448, 571
XspI CTAG 5 cut(s) 131, 231, 449, 572, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.