Rmu_sc0009745.1_g000015

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009745.1
OG0000882
Physical Location & Seq
Forward (+)
45817 .. 50701
4885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009745.1_g000015.1.cds

Sequence Viewer

Length: 2556 bp
atggccgccggcatggagaacaataattggggtttcgggtcgatctggattgttgcacaagagttagttgggtatggatataaagtcgagtctgtgaataattctgattcctcggggaagacattgaccgttaaccttggtctcatcaacagctcctcagtttatggacctgacatatcaaatcttaccctctatgtcagctatgagaccaaagaccgtctaagagttcgaataacagactcaacgcaacaaagatgggagctcccacaagacattctcccccgccaaaaccatacttcacagaacttttccgtcactgacaacgacctcgttttcaccctccgcaacccccccccgttcggcttcaccatcccccgccgctcctctaacgacaccgttttcgacaccaccccgaacccctccgaccccaacaccaccttcatcttcaaggaccaatacttccagctctcctcctctcttcccaacaacaggtcctccctgtacggcctcggagagcacaccaagagcaccttcaagctcctagccaatcagactctgacactctggagcgccgacatcggcagcgtgagtcccgacctcaatctctatgggtcccaccccttctacatggacgtaagatcgccgtccgatgatggcaaggtcaaggcgggaaccactcacggagtgctgcttttgaacagcaatggcatggatgtaaattacacaggtgacagggttacttataatgtgattggcggagttgtggacttgtatttcttttcgggtccgacacccgaattggtgatggagcagtacactgagctcattggtcgacccgcccctatgccatattggtctttcggattccaccagtgtagatatggttacaaggatgtttctgatcttgagggggtcgttgctggttatgcaaatgctagtatccctcttgaggttatgtggacagacattgattacatggatgcttacaaggatttcactcttgatcccgtcaactttccattagacaggatgaagaactttaccaacaaccttcaccaaaatggtcagaaatacgtgcttatcttggatcccggtattagtatcaatgatagctatggaacctacaccagagggaaggaagcagatatatttataaaacgtgatggcattccttaccagggaaatgtttggcctggcaatgtttactttcctgattttgtaaatccacaaagtgaaccatattgggagaatgagattaaactatttctagaccagctacctgttgatggcctttggcttgatatgaatgaagtatcaaatttccaaacttctaatcccactccgaattccacacttgatgatccaccttacaaaatcaacgatgctggaggccaccgtccgatccttagtaggactattccaggatcagctctacactttggtaatgttacagagtataatgttcataacctatttggcatgttagaagccaaagcaacaaacaaggccttgaccaatttgactggtaagagaccttttgttctctcgagatcgacttttgtaagctctggtaagtacacagcacactggactggagacaatgcagcaagatggagtgacttggcatacacaattccaggcattttgaattttggactcttcggagttccaatggttggagctgatatatgtggtttctctggggatactaatgaagaactctgccggcgttggattcagctaggggccttttaccccttttcaagagatcattcggataagaattcaatccggcaggagctctatgtttgggaatcagtggctgcatcagcaaaaaaagtgctggggctacgttaccggttgcttcccttgttctatacatcaatgtatcaagcacacaagaacgggactccaattgcaaggcccgtattcttctcatttcctgaagataccaatacatacagtatctattcacagtttctgattggtaaaggcgtaatggtgtcacctgtgttgcagcaaggagcaaattcagttgaggcatattttcctgcaggaaattggttcgatctcttcaactacacatcagtcagtgtagactctggaaagaatgttacgttggatgcaccgcctgatcatatcaatgtgcatgtccacgaaggaaacattttggccttgcaaggagaggccttgacaacacaagcagcccgaaacactacattcgaacttttggtggtcactggtagtagcaatgagagtaccggagaattgttcttggatgatggagaggaagtcgaaatgggaggagagggagggaagtggagttttgtgaggtttcgttctgcacgagcacaaaatggaagtctagttttgagctcaaaggtcgaaaatggagaatttgcattaagccaaggttggatcattgataaaattaccgtccttgggttggacaaagttaatgcaacacagtccagtgtatcccttcctataggaaaggagtttgagttggagctgaagccgaatcagacactggcgaacccaaaaacatatttatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

851

Amino Acids

94.76

Weight (kDa)

5.05

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 744, 1154
AccB7I CCANNNNNTGG 1 cut(s) 1676
AccBSI CCGCTC 1 cut(s) 381
AccI GTMKAC 2 cut(s) 832, 2097
AciI CCGC 9 cut(s) 6, 283, 343, 376, 379, 668, 756, 837, 2129
AclWI GGATC 7 cut(s) 998, 1082, 1095, 1355, 1396, 1432, 2426
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 6 cut(s) 1318, 1345, 1648, 1783, 2029, 2396
AcuI CTGAAG 2 cut(s) 1965, 2534
AdeI CACNNNGTG 2 cut(s) 685, 1232
AfaI GTAC 4 cut(s) 503, 815, 1577, 2260
AfiI CCNNNNNNNGG 8 cut(s) 359, 489, 949, 1178, 1286, 1676, 2442, 2446
AgeI ACCGGT 1 cut(s) 1857
AgsI TTSAA 7 cut(s) 448, 535, 697, 1648, 1764, 1788, 2077
AjnI CCWGG 4 cut(s) 1176, 1192, 1420, 1636
AjuI GAANNNNNNNTTGG 6 cut(s) 422, 454, 515, 547, 2102, 2134
Alw21I GWGCWC 7 cut(s) 264, 519, 530, 825, 1803, 2353, 2378
Alw26I GTCTC 4 cut(s) 146, 200, 1525, 1590
AlwI GGATC 7 cut(s) 998, 1082, 1095, 1355, 1396, 1432, 2426
AlwNI CAGNNNCTG 4 cut(s) 556, 1823, 1981, 2530
Ama87I CYCGRG 2 cut(s) 112, 1546
ApeKI GCWGC 6 cut(s) 582, 688, 1604, 1823, 2017, 2204
ApoI RAATTY 6 cut(s) 1318, 1345, 1648, 1783, 2029, 2396
ArsI GACNNNNNNTTYG 4 cut(s) 383, 415, 2347, 2379
AsiGI ACCGGT 1 cut(s) 1857
AspLEI GCGC 1 cut(s) 572
AspS9I GGNCC 7 cut(s) 167, 451, 492, 612, 785, 1746, 1923
AsuC2I CCSGG 1 cut(s) 1092
AsuHPI GGTGA 6 cut(s) 328, 358, 740, 814, 1046, 1998
AsuII TTCGAA 2 cut(s) 229, 2223
AvaI CYCGRG 2 cut(s) 112, 1546
AvaII GGWCC 5 cut(s) 167, 451, 492, 612, 785
BamHI GGATCC 1 cut(s) 1087
BanII GRGCYC 4 cut(s) 264, 825, 1803, 2378
BauI CACGAG 1 cut(s) 2346
BbsI GAAGAC 1 cut(s) 125
Bbv12I GWGCWC 7 cut(s) 264, 519, 530, 825, 1803, 2353, 2378
BbvI GCAGC 6 cut(s) 594, 675, 1616, 1810, 2029, 2216
BccI CCATC 8 cut(s) 249, 377, 647, 799, 1157, 1280, 1605, 2276
BceAI ACGGC 2 cut(s) 520, 628
BciT130I CCWGG 4 cut(s) 1178, 1194, 1422, 1638
BciVI GTATCC 3 cut(s) 950, 1699, 2488
BclI TGATCA 1 cut(s) 2134
BcnI CCSGG 1 cut(s) 1092
BcoDI GTCTC 4 cut(s) 146, 200, 1525, 1590
BfaI CTAG 5 cut(s) 542, 936, 1268, 1742, 2366
BfmI CTRYAG 2 cut(s) 2052, 2487
BfoI RGCGCY 1 cut(s) 573
BfuI GTATCC 3 cut(s) 950, 1699, 2488
BisI GCNGC 8 cut(s) 6, 379, 583, 689, 1605, 1824, 2018, 2205
BlsI GCNGC 8 cut(s) 7, 380, 584, 690, 1606, 1825, 2019, 2206
Bme1390I CCNGG 5 cut(s) 1092, 1178, 1194, 1422, 1638
Bme18I GGWCC 5 cut(s) 167, 451, 492, 612, 785
BmeT110I CYCGRG 2 cut(s) 112, 1546
BmgT120I GGNCC 7 cut(s) 167, 451, 492, 612, 785, 1746, 1923
BmiI GGNNCC 7 cut(s) 613, 614, 673, 786, 1089, 1120, 1747
BmrFI CCNGG 5 cut(s) 1092, 1178, 1194, 1422, 1638
BmsI GCATC 4 cut(s) 970, 1372, 1835, 2113
BpiI GAAGAC 1 cut(s) 125
BpmI CTGGAG 3 cut(s) 586, 1407, 1614
Bpu14I TTCGAA 2 cut(s) 229, 2223
BpuEI CTTGAG 2 cut(s) 926, 968
BpuMI CCSGG 1 cut(s) 1092
BsaAI YACGTR 1 cut(s) 1075
BsaI GGTCTC 3 cut(s) 146, 200, 1525
BsaJI CCNNGG 6 cut(s) 111, 136, 508, 1177, 2410, 2440
BsaWI WCCGGW 2 cut(s) 1857, 2261
BsaXI ACNNNNNCTCC 6 cut(s) 479, 509, 2277, 2307, 2489, 2519
Bsc4I CCNNNNNNNGG 8 cut(s) 359, 489, 949, 1178, 1286, 1676, 2442, 2446
Bse118I RCCGGY 3 cut(s) 8, 1725, 1857
Bse1I ACTGG 7 cut(s) 871, 1528, 1592, 1597, 2245, 2472, 2535
Bse3DI GCAATG 3 cut(s) 709, 1204, 2257
BseBI CCWGG 4 cut(s) 1178, 1194, 1422, 1638
BseDI CCNNGG 6 cut(s) 111, 136, 508, 1177, 2410, 2440
BseGI GGATG 7 cut(s) 369, 718, 898, 985, 1035, 2128, 2284
BseLI CCNNNNNNNGG 8 cut(s) 359, 489, 949, 1178, 1286, 1676, 2442, 2446
BseMI GCAATG 3 cut(s) 709, 1204, 2257
BseMII CTCAG 2 cut(s) 171, 810
BseNI ACTGG 7 cut(s) 871, 1528, 1592, 1597, 2245, 2472, 2535
BseRI GAGGAG 5 cut(s) 145, 373, 460, 463, 2319
BseXI GCAGC 6 cut(s) 594, 675, 1616, 1810, 2029, 2216
BseYI CCCAGC 1 cut(s) 1843
BsgI GTGCAG 1 cut(s) 2328
BshTI ACCGGT 1 cut(s) 1857
BsiHKAI GWGCWC 7 cut(s) 264, 519, 530, 825, 1803, 2353, 2378
BsiHKCI CYCGRG 2 cut(s) 112, 1546
BsiSI CCGG 6 cut(s) 9, 1092, 1726, 1792, 1858, 2262
BslFI GGGAC 3 cut(s) 576, 598, 1921
BslI CCNNNNNNNGG 8 cut(s) 359, 489, 949, 1178, 1286, 1676, 2442, 2446
BsmAI GTCTC 4 cut(s) 146, 200, 1525, 1590
BsmFI GGGAC 3 cut(s) 576, 598, 1921
BsmI GAATGC 1 cut(s) 1167
Bso31I GGTCTC 3 cut(s) 146, 200, 1525
BsoBI CYCGRG 2 cut(s) 112, 1546
Bsp119I TTCGAA 2 cut(s) 229, 2223
Bsp1286I GDGCHC 7 cut(s) 264, 519, 530, 825, 1803, 2353, 2378
BspACI CCGC 9 cut(s) 6, 283, 343, 376, 379, 668, 756, 837, 2129
BspCNI CTCAG 2 cut(s) 170, 811
BspLI GGNNCC 7 cut(s) 613, 614, 673, 786, 1089, 1120, 1747
BspMAI CTGCAG 1 cut(s) 2056
BspPI GGATC 7 cut(s) 998, 1082, 1095, 1355, 1396, 1432, 2426
BspT104I TTCGAA 2 cut(s) 229, 2223
BspTNI GGTCTC 3 cut(s) 146, 200, 1525
BsrBI CCGCTC 1 cut(s) 381
BsrDI GCAATG 3 cut(s) 709, 1204, 2257
BsrFI RCCGGY 3 cut(s) 8, 1725, 1857
BsrI ACTGG 7 cut(s) 871, 1528, 1592, 1597, 2245, 2472, 2535
BssAI RCCGGY 3 cut(s) 8, 1725, 1857
BssECI CCNNGG 6 cut(s) 111, 136, 508, 1177, 2410, 2440
BssSI CACGAG 1 cut(s) 2346
BssT1I CCWWGG 3 cut(s) 136, 2410, 2440
Bst2BI CACGAG 1 cut(s) 2346
Bst2UI CCWGG 4 cut(s) 1178, 1194, 1422, 1638
Bst4CI ACNGT 8 cut(s) 130, 218, 397, 1397, 1964, 1977, 2437, 2469
Bst6I CTCTTC 3 cut(s) 483, 1664, 2078
BstBAI YACGTR 1 cut(s) 1075
BstBI TTCGAA 2 cut(s) 229, 2223
BstC8I GCNNGC 2 cut(s) 10, 1727
BstDEI CTNAG 4 cut(s) 157, 221, 819, 1406
BstF5I GGATG 7 cut(s) 369, 718, 898, 985, 1035, 2128, 2284
BstH2I RGCGCY 1 cut(s) 573
BstHHI GCGC 1 cut(s) 572
BstMAI GTCTC 4 cut(s) 146, 200, 1525, 1590
BstMWI GCNNNNNNNGC 2 cut(s) 926, 1829
BstNI CCWGG 4 cut(s) 1178, 1194, 1422, 1638
BstNSI RCATGY 2 cut(s) 1483, 2153
BstSCI CCNGG 5 cut(s) 1090, 1176, 1192, 1420, 1636
BstSFI CTRYAG 2 cut(s) 2052, 2487
BstV1I GCAGC 6 cut(s) 594, 675, 1616, 1810, 2029, 2216
BstV2I GAAGAC 1 cut(s) 125
BstX2I RGATCY 1 cut(s) 1087
BstYI RGATCY 1 cut(s) 1087
BsuI GTATCC 3 cut(s) 950, 1699, 2488
BtsCI GGATG 7 cut(s) 369, 718, 898, 985, 1035, 2128, 2284
BtsIMutI CAGTG 9 cut(s) 315, 816, 878, 1585, 1824, 2098, 2238, 2479, 2528
Cac8I GCNNGC 2 cut(s) 10, 1727
CaiI CAGNNNCTG 4 cut(s) 556, 1823, 1981, 2530
CfoI GCGC 1 cut(s) 572
Cfr10I RCCGGY 3 cut(s) 8, 1725, 1857
Cfr13I GGNCC 7 cut(s) 167, 451, 492, 612, 785, 1746, 1923
Csp6I GTAC 4 cut(s) 502, 814, 1576, 2259
CspAI ACCGGT 1 cut(s) 1857
CviAII CATG 6 cut(s) 13, 628, 709, 976, 1480, 2150
CviQI GTAC 4 cut(s) 502, 814, 1576, 2259
DdeI CTNAG 4 cut(s) 157, 221, 819, 1406
DraIII CACNNNGTG 2 cut(s) 685, 1232
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 483, 1664, 2078
EarI CTCTTC 3 cut(s) 483, 1664, 2078
EciI GGCGGA 1 cut(s) 771
Ecl136II GAGCTC 4 cut(s) 262, 823, 1801, 2376
Eco130I CCWWGG 3 cut(s) 136, 2410, 2440
Eco147I AGGCCT 2 cut(s) 1508, 2189
Eco24I GRGCYC 4 cut(s) 264, 825, 1803, 2378
Eco31I GGTCTC 3 cut(s) 146, 200, 1525
Eco47I GGWCC 5 cut(s) 167, 451, 492, 612, 785
Eco53kI GAGCTC 4 cut(s) 262, 823, 1801, 2376
Eco57I CTGAAG 2 cut(s) 1965, 2534
Eco88I CYCGRG 2 cut(s) 112, 1546
EcoICRI GAGCTC 4 cut(s) 262, 823, 1801, 2376
EcoO109I RGGNCCY 3 cut(s) 492, 612, 1746
EcoRI GAATTC 2 cut(s) 1345, 1783
EcoRII CCWGG 4 cut(s) 1176, 1192, 1420, 1636
EcoT14I CCWWGG 3 cut(s) 136, 2410, 2440
EcoT38I GRGCYC 4 cut(s) 264, 825, 1803, 2378
ErhI CCWWGG 3 cut(s) 136, 2410, 2440
FaeI CATG 6 cut(s) 16, 631, 712, 979, 1483, 2153
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 3 cut(s) 576, 598, 1921
FatI CATG 6 cut(s) 12, 627, 708, 975, 1479, 2149
FauI CCCGC 4 cut(s) 290, 383, 661, 844
FbaI TGATCA 1 cut(s) 2134
FblI GTMKAC 2 cut(s) 832, 2097
Fnu4HI GCNGC 8 cut(s) 6, 379, 583, 689, 1605, 1824, 2018, 2205
FokI GGATG 7 cut(s) 356, 725, 905, 992, 1042, 2135, 2291
FriOI GRGCYC 4 cut(s) 264, 825, 1803, 2378
Fsp4HI GCNGC 8 cut(s) 6, 379, 583, 689, 1605, 1824, 2018, 2205
FspBI CTAG 5 cut(s) 542, 936, 1268, 1742, 2366
GlaI GCGC 1 cut(s) 571
GluI GCNGC 8 cut(s) 6, 379, 583, 689, 1605, 1824, 2018, 2205
GsaI CCCAGC 1 cut(s) 1847
GsuI CTGGAG 3 cut(s) 586, 1407, 1614
HaeII RGCGCY 1 cut(s) 573
HapII CCGG 6 cut(s) 9, 1092, 1726, 1792, 1858, 2262
HhaI GCGC 1 cut(s) 572
Hin1II CATG 6 cut(s) 16, 631, 712, 979, 1483, 2153
Hin6I GCGC 1 cut(s) 570
HinP1I GCGC 1 cut(s) 570
HincII GTYRAC 3 cut(s) 133, 833, 1012
HindII GTYRAC 3 cut(s) 133, 833, 1012
HpaI GTTAAC 1 cut(s) 133
HpaII CCGG 6 cut(s) 9, 1092, 1726, 1792, 1858, 2262
HphI GGTGA 6 cut(s) 328, 358, 740, 814, 1046, 1998
HpyAV CCTTC 7 cut(s) 448, 541, 631, 1061, 1129, 2153, 2492
HpyCH4III ACNGT 8 cut(s) 130, 218, 397, 1397, 1964, 1977, 2437, 2469
HpyCH4IV ACGT 5 cut(s) 633, 1074, 1159, 1852, 2117
HpyF10VI GCNNNNNNNGC 2 cut(s) 926, 1829
HpyF3I CTNAG 4 cut(s) 157, 221, 819, 1406
HpySE526I ACGT 5 cut(s) 633, 1074, 1159, 1852, 2117
Hsp92II CATG 6 cut(s) 16, 631, 712, 979, 1483, 2153
HspAI GCGC 1 cut(s) 570
KflI GGGWCCC 1 cut(s) 612
KroI GCCGGC 2 cut(s) 8, 1725
KroNI GCCGGC 2 cut(s) 10, 1727
Ksp22I TGATCA 1 cut(s) 2134
KspAI GTTAAC 1 cut(s) 133
Lsp1109I GCAGC 6 cut(s) 594, 675, 1616, 1810, 2029, 2216
LweI GCATC 4 cut(s) 970, 1372, 1835, 2113
MaeI CTAG 5 cut(s) 542, 936, 1268, 1742, 2366
MaeII ACGT 5 cut(s) 633, 1074, 1159, 1852, 2117
MbiI CCGCTC 1 cut(s) 381
MboII GAAGA 9 cut(s) 130, 436, 470, 1045, 1651, 1727, 1924, 1958, 2065
MfeI CAATTG 1 cut(s) 1914
MflI RGATCY 1 cut(s) 1087
MhlI GDGCHC 7 cut(s) 264, 519, 530, 825, 1803, 2353, 2378
MlyI GAGTC 7 cut(s) 98, 233, 547, 598, 1650, 1903, 2093
MmeI TCCRAC 8 cut(s) 447, 812, 1657, 1712, 2100, 2396, 2427, 2487
MroNI GCCGGC 2 cut(s) 8, 1725
MseI TTAA 4 cut(s) 132, 1257, 2405, 2457
MslI CAYNNNNRTG 3 cut(s) 1059, 2142, 2551
MspI CCGG 6 cut(s) 9, 1092, 1726, 1792, 1858, 2262
MspR9I CCNGG 5 cut(s) 1092, 1178, 1194, 1422, 1638
MunI CAATTG 1 cut(s) 1914
Mva1269I GAATGC 1 cut(s) 1167
MvaI CCWGG 4 cut(s) 1178, 1194, 1422, 1638
MwoI GCNNNNNNNGC 2 cut(s) 926, 1829
NaeI GCCGGC 2 cut(s) 10, 1727
NciI CCSGG 1 cut(s) 1092
NgoMIV GCCGGC 2 cut(s) 8, 1725
NlaIII CATG 6 cut(s) 16, 631, 712, 979, 1483, 2153
NlaIV GGNNCC 7 cut(s) 613, 614, 673, 786, 1089, 1120, 1747
NmuCI GTSAC 5 cut(s) 313, 728, 1616, 2004, 2236
NspI RCATGY 2 cut(s) 1483, 2153
NspV TTCGAA 2 cut(s) 229, 2223
PaeR7I CTCGAG 1 cut(s) 1546
PceI AGGCCT 2 cut(s) 1508, 2189
PcsI WCGNNNNNNNCGW 1 cut(s) 2344
PctI GAATGC 1 cut(s) 1167
PdiI GCCGGC 2 cut(s) 10, 1727
PfeI GAWTC 5 cut(s) 107, 864, 1735, 1814, 2521
PflMI CCANNNNNTGG 1 cut(s) 1676
PfoI TCCNGGA 1 cut(s) 1420
PinAI ACCGGT 1 cut(s) 1857
PkrI GCNGC 8 cut(s) 7, 380, 584, 690, 1606, 1825, 2019, 2206
PleI GAGTC 7 cut(s) 97, 233, 547, 597, 1650, 1903, 2093
PpsI GAGTC 7 cut(s) 97, 233, 547, 597, 1650, 1903, 2093
Ppu21I YACGTR 1 cut(s) 1075
PpuMI RGGWCCY 2 cut(s) 492, 612
PsiI TTATAA 2 cut(s) 744, 1154
Psp124BI GAGCTC 4 cut(s) 264, 825, 1803, 2378
Psp5II RGGWCCY 2 cut(s) 492, 612
Psp6I CCWGG 4 cut(s) 1176, 1192, 1420, 1636
PspFI CCCAGC 1 cut(s) 1843
PspGI CCWGG 4 cut(s) 1176, 1192, 1420, 1636
PspN4I GGNNCC 7 cut(s) 613, 614, 673, 786, 1089, 1120, 1747
PspPI GGNCC 7 cut(s) 167, 451, 492, 612, 785, 1746, 1923
PspPPI RGGWCCY 2 cut(s) 492, 612
PstI CTGCAG 1 cut(s) 2056
PstNI CAGNNNCTG 4 cut(s) 556, 1823, 1981, 2530
PsuI RGATCY 1 cut(s) 1087
RsaI GTAC 4 cut(s) 503, 815, 1577, 2260
RsaNI GTAC 4 cut(s) 502, 814, 1576, 2259
RseI CAYNNNNRTG 3 cut(s) 1059, 2142, 2551
SacI GAGCTC 4 cut(s) 264, 825, 1803, 2378
SalI GTCGAC 1 cut(s) 831
SaqAI TTAA 4 cut(s) 132, 1257, 2405, 2457
SatI GCNGC 8 cut(s) 6, 379, 583, 689, 1605, 1824, 2018, 2205
Sau96I GGNCC 7 cut(s) 167, 451, 492, 612, 785, 1746, 1923
SbfI CCTGCAGG 1 cut(s) 2056
SchI GAGTC 7 cut(s) 98, 233, 547, 598, 1650, 1903, 2093
ScrFI CCNGG 5 cut(s) 1092, 1178, 1194, 1422, 1638
SdaI CCTGCAGG 1 cut(s) 2056
SduI GDGCHC 7 cut(s) 264, 519, 530, 825, 1803, 2353, 2378
SfaNI GCATC 4 cut(s) 970, 1372, 1835, 2113
SfcI CTRYAG 2 cut(s) 2052, 2487
Sfr274I CTCGAG 1 cut(s) 1546
SfuI TTCGAA 2 cut(s) 229, 2223
SinI GGWCC 5 cut(s) 167, 451, 492, 612, 785
SlaI CTCGAG 1 cut(s) 1546
SmiMI CAYNNNNRTG 3 cut(s) 1059, 2142, 2551
SmlI CTYRAG 3 cut(s) 905, 947, 1546
SmoI CTYRAG 3 cut(s) 905, 947, 1546
Sse8387I CCTGCAGG 1 cut(s) 2056
SseBI AGGCCT 2 cut(s) 1508, 2189
SsiI CCGC 9 cut(s) 6, 283, 343, 376, 379, 668, 756, 837, 2129
SspMI CTAG 5 cut(s) 542, 936, 1268, 1742, 2366
SstI GAGCTC 4 cut(s) 264, 825, 1803, 2378
StuI AGGCCT 2 cut(s) 1508, 2189
StyD4I CCNGG 5 cut(s) 1090, 1176, 1192, 1420, 1636
StyI CCWWGG 3 cut(s) 136, 2410, 2440
TaaI ACNGT 8 cut(s) 130, 218, 397, 1397, 1964, 1977, 2437, 2469
TaiI ACGT 5 cut(s) 636, 1077, 1162, 1855, 2120
TatI WGTACW 2 cut(s) 813, 1575
TauI GCSGC 2 cut(s) 8, 381
TfiI GAWTC 5 cut(s) 107, 864, 1735, 1814, 2521
Tru1I TTAA 4 cut(s) 132, 1257, 2405, 2457
Tru9I TTAA 4 cut(s) 132, 1257, 2405, 2457
TscAI CASTG 9 cut(s) 322, 823, 878, 1592, 1824, 2098, 2245, 2479, 2535
TseFI GTSAC 5 cut(s) 313, 728, 1616, 2004, 2236
TseI GCWGC 6 cut(s) 582, 688, 1604, 1823, 2017, 2204
Tsp45I GTSAC 5 cut(s) 313, 728, 1616, 2004, 2236
TspDTI ATGAA 6 cut(s) 430, 1046, 1319, 1323, 1454, 1728
TspGWI ACGGA 2 cut(s) 301, 696
TspRI CASTG 9 cut(s) 322, 823, 878, 1592, 1824, 2098, 2245, 2479, 2535
Van91I CCANNNNNTGG 1 cut(s) 1676
VpaK11BI GGWCC 5 cut(s) 167, 451, 492, 612, 785
XapI RAATTY 6 cut(s) 1318, 1345, 1648, 1783, 2029, 2396
XbaI TCTAGA 1 cut(s) 1267
XceI RCATGY 2 cut(s) 1483, 2153
XcmI CCANNNNNNNNNTGG 1 cut(s) 878
XhoI CTCGAG 1 cut(s) 1546
XmiI GTMKAC 2 cut(s) 832, 2097
XspI CTAG 5 cut(s) 542, 936, 1268, 1742, 2366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.