Belongs to the glycosyl hydrolase 31 family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Gal_mutarotas_2 | PF13802 | 87 - 247 | 2e-10 | Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain |
| Glyco_hydro_31_2nd | PF01055 | 292 - 640 | 6.9e-120 | Glycosyl hydrolases family 31 TIM-barrel domain |
| Glyco_hydro_31_3rd | PF21365 | 649 - 738 | 4.4e-24 | Glycosyl hydrolase family 31 C-terminal domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 783, 1364 |
| AatII | GACGTC | 1 cut(s) | 435 |
| Acc36I | ACCTGC | 1 cut(s) | 883 |
| AccI | GTMKAC | 2 cut(s) | 2139, 2516 |
| AccII | CGCG | 1 cut(s) | 707 |
| AccIII | TCCGGA | 2 cut(s) | 1424, 2144 |
| AciI | CCGC | 7 cut(s) | 415, 459, 705, 707, 1831, 2171, 2292 |
| AclWI | GGATC | 4 cut(s) | 1037, 1394, 1822, 2468 |
| AcoI | YGGCCR | 2 cut(s) | 868, 1102 |
| AcsI | RAATTY | 4 cut(s) | 264, 1687, 2068, 2438 |
| AcuI | CTGAAG | 3 cut(s) | 1773, 2004, 2672 |
| AcyI | GRCGYC | 2 cut(s) | 432, 830 |
| AdeI | CACNNNGTG | 1 cut(s) | 1271 |
| AfaI | GTAC | 8 cut(s) | 116, 497, 542, 854, 1260, 1616, 2190, 2383 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 295, 1174, 1217, 1715, 1959, 2484, 2488 |
| AflIII | ACRYGT | 1 cut(s) | 2191 |
| AgeI | ACCGGT | 1 cut(s) | 1896 |
| AgsI | TTSAA | 9 cut(s) | 487, 574, 736, 989, 1307, 1687, 1744, 2116, 2548 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 676 |
| AjiI | CACGTC | 1 cut(s) | 673 |
| AjnI | CCWGG | 4 cut(s) | 1129, 1231, 1542, 1675 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 93, 125, 554, 586 |
| Alw21I | GWGCWC | 5 cut(s) | 864, 1119, 2303, 2420, 2528 |
| Alw26I | GTCTC | 5 cut(s) | 81, 1334, 1564, 1629, 1800 |
| AlwI | GGATC | 4 cut(s) | 1037, 1394, 1822, 2468 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 595, 1862, 2668 |
| Ama87I | CYCGRG | 2 cut(s) | 820, 2483 |
| Aor13HI | TCCGGA | 2 cut(s) | 1424, 2144 |
| ApeKI | GCWGC | 4 cut(s) | 1178, 1643, 1862, 2246 |
| ApoI | RAATTY | 4 cut(s) | 264, 1687, 2068, 2438 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 249, 281, 422, 454, 2389, 2421 |
| AsiGI | ACCGGT | 1 cut(s) | 1896 |
| AspLEI | GCGC | 1 cut(s) | 626 |
| AsuC2I | CCSGG | 4 cut(s) | 154, 521, 529, 1217 |
| AsuII | TTCGAA | 2 cut(s) | 268, 2265 |
| AsuNHI | GCTAGC | 1 cut(s) | 974 |
| AvaI | CYCGRG | 2 cut(s) | 820, 2483 |
| AvaII | GGWCC | 8 cut(s) | 206, 490, 531, 651, 824, 1126, 1947, 2607 |
| BalI | TGGCCA | 1 cut(s) | 1104 |
| BanII | GRGCYC | 2 cut(s) | 864, 2420 |
| BauI | CACGAG | 1 cut(s) | 1803 |
| BbsI | GAAGAC | 3 cut(s) | 164, 475, 2261 |
| Bbv12I | GWGCWC | 5 cut(s) | 864, 1119, 2303, 2420, 2528 |
| BbvI | GCAGC | 4 cut(s) | 1190, 1655, 1849, 2258 |
| BceAI | ACGGC | 4 cut(s) | 183, 402, 559, 2017 |
| BciT130I | CCWGG | 4 cut(s) | 1131, 1233, 1544, 1677 |
| BciVI | GTATCC | 3 cut(s) | 113, 1363, 2626 |
| BclI | TGATCA | 3 cut(s) | 183, 1551, 2176 |
| BcnI | CCSGG | 4 cut(s) | 154, 521, 529, 1217 |
| BcoDI | GTCTC | 5 cut(s) | 81, 1334, 1564, 1629, 1800 |
| BfaI | CTAG | 8 cut(s) | 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683 |
| BfmI | CTRYAG | 1 cut(s) | 2625 |
| BfoI | RGCGCY | 1 cut(s) | 627 |
| BfuAI | ACCTGC | 1 cut(s) | 883 |
| BfuI | GTATCC | 3 cut(s) | 113, 1363, 2626 |
| BisI | GCNGC | 4 cut(s) | 1179, 1644, 1863, 2247 |
| BlsI | GCNGC | 4 cut(s) | 1180, 1645, 1864, 2248 |
| Bme1390I | CCNGG | 8 cut(s) | 154, 521, 529, 1131, 1217, 1233, 1544, 1677 |
| Bme18I | GGWCC | 8 cut(s) | 206, 490, 531, 651, 824, 1126, 1947, 2607 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 676 |
| BmeT110I | CYCGRG | 2 cut(s) | 820, 2483 |
| BmgBI | CACGTC | 1 cut(s) | 673 |
| BmrFI | CCNGG | 8 cut(s) | 154, 521, 529, 1131, 1217, 1233, 1544, 1677 |
| BmsI | GCATC | 7 cut(s) | 987, 1009, 1059, 1472, 1874, 2013, 2155 |
| BmtI | GCTAGC | 1 cut(s) | 978 |
| BpiI | GAAGAC | 3 cut(s) | 164, 475, 2261 |
| BpmI | CTGGAG | 3 cut(s) | 1653, 2107, 2325 |
| Bpu14I | TTCGAA | 2 cut(s) | 268, 2265 |
| BpuEI | CTTGAG | 1 cut(s) | 209 |
| BpuMI | CCSGG | 4 cut(s) | 154, 521, 529, 1217 |
| BsaHI | GRCGYC | 2 cut(s) | 432, 830 |
| BsaI | GGTCTC | 3 cut(s) | 1334, 1564, 1800 |
| BsaJI | CCNNGG | 8 cut(s) | 547, 705, 1129, 1168, 1216, 1543, 2452, 2482 |
| BsaWI | WCCGGW | 4 cut(s) | 686, 1424, 1896, 2144 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 295, 1174, 1217, 1715, 1959, 2484, 2488 |
| Bse118I | RCCGGY | 2 cut(s) | 1764, 1896 |
| Bse3DI | GCAATG | 2 cut(s) | 615, 1953 |
| BseAI | TCCGGA | 2 cut(s) | 1424, 2144 |
| BseBI | CCWGG | 4 cut(s) | 1131, 1233, 1544, 1677 |
| BseDI | CCNNGG | 8 cut(s) | 547, 705, 1129, 1168, 1216, 1543, 2452, 2482 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 295, 1174, 1217, 1715, 1959, 2484, 2488 |
| BseMI | GCAATG | 2 cut(s) | 615, 1953 |
| BseMII | CTCAG | 1 cut(s) | 210 |
| BseRI | GAGGAG | 4 cut(s) | 184, 502, 1362, 2361 |
| BseXI | GCAGC | 4 cut(s) | 1190, 1655, 1849, 2258 |
| BsgI | GTGCAG | 1 cut(s) | 2370 |
| Bsh1236I | CGCG | 1 cut(s) | 707 |
| BshTI | ACCGGT | 1 cut(s) | 1896 |
| BsiHKAI | GWGCWC | 5 cut(s) | 864, 1119, 2303, 2420, 2528 |
| BsiHKCI | CYCGRG | 2 cut(s) | 820, 2483 |
| BslFI | GGGAC | 2 cut(s) | 637, 1960 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 295, 1174, 1217, 1715, 1959, 2484, 2488 |
| BsmAI | GTCTC | 5 cut(s) | 81, 1334, 1564, 1629, 1800 |
| BsmFI | GGGAC | 2 cut(s) | 637, 1960 |
| BsmI | GAATGC | 1 cut(s) | 2261 |
| Bso31I | GGTCTC | 3 cut(s) | 1334, 1564, 1800 |
| BsoBI | CYCGRG | 2 cut(s) | 820, 2483 |
| Bsp119I | TTCGAA | 2 cut(s) | 268, 2265 |
| Bsp1286I | GDGCHC | 5 cut(s) | 864, 1119, 2303, 2420, 2528 |
| Bsp13I | TCCGGA | 2 cut(s) | 1424, 2144 |
| Bsp1407I | TGTACA | 2 cut(s) | 1258, 2188 |
| BspACI | CCGC | 7 cut(s) | 415, 459, 705, 707, 1831, 2171, 2292 |
| BspCNI | CTCAG | 1 cut(s) | 209 |
| BspEI | TCCGGA | 2 cut(s) | 1424, 2144 |
| BspFNI | CGCG | 1 cut(s) | 707 |
| BspMI | ACCTGC | 1 cut(s) | 883 |
| BspOI | GCTAGC | 1 cut(s) | 978 |
| BspPI | GGATC | 4 cut(s) | 1037, 1394, 1822, 2468 |
| BspT104I | TTCGAA | 2 cut(s) | 268, 2265 |
| BspTNI | GGTCTC | 3 cut(s) | 1334, 1564, 1800 |
| BsrDI | GCAATG | 2 cut(s) | 615, 1953 |
| BsrFI | RCCGGY | 2 cut(s) | 1764, 1896 |
| BsrGI | TGTACA | 2 cut(s) | 1258, 2188 |
| BssAI | RCCGGY | 2 cut(s) | 1764, 1896 |
| BssECI | CCNNGG | 8 cut(s) | 547, 705, 1129, 1168, 1216, 1543, 2452, 2482 |
| BssNAI | GTATAC | 1 cut(s) | 2517 |
| BssNI | GRCGYC | 2 cut(s) | 432, 830 |
| BssSI | CACGAG | 1 cut(s) | 1803 |
| BssT1I | CCWWGG | 1 cut(s) | 2452 |
| Bst1107I | GTATAC | 1 cut(s) | 2517 |
| Bst2BI | CACGAG | 1 cut(s) | 1803 |
| Bst2UI | CCWGG | 4 cut(s) | 1131, 1233, 1544, 1677 |
| Bst4CI | ACNGT | 6 cut(s) | 409, 684, 1456, 2016, 2479, 2582 |
| Bst6I | CTCTTC | 2 cut(s) | 1703, 2117 |
| BstACI | GRCGYC | 2 cut(s) | 432, 830 |
| BstAUI | TGTACA | 2 cut(s) | 1258, 2188 |
| BstBI | TTCGAA | 2 cut(s) | 268, 2265 |
| BstC8I | GCNNGC | 4 cut(s) | 238, 622, 976, 1766 |
| BstDEI | CTNAG | 2 cut(s) | 196, 260 |
| BstDSI | CCRYGG | 1 cut(s) | 705 |
| BstEII | GGTNACC | 1 cut(s) | 678 |
| BstFNI | CGCG | 1 cut(s) | 707 |
| BstH2I | RGCGCY | 1 cut(s) | 627 |
| BstHHI | GCGC | 1 cut(s) | 626 |
| BstMAI | GTCTC | 5 cut(s) | 81, 1334, 1564, 1629, 1800 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 617, 965, 1868, 2298 |
| BstNI | CCWGG | 4 cut(s) | 1131, 1233, 1544, 1677 |
| BstNSI | RCATGY | 1 cut(s) | 2195 |
| BstPI | GGTNACC | 1 cut(s) | 678 |
| BstSCI | CCNGG | 8 cut(s) | 152, 519, 527, 1129, 1215, 1231, 1542, 1675 |
| BstSFI | CTRYAG | 1 cut(s) | 2625 |
| BstUI | CGCG | 1 cut(s) | 707 |
| BstV1I | GCAGC | 4 cut(s) | 1190, 1655, 1849, 2258 |
| BstV2I | GAAGAC | 3 cut(s) | 164, 475, 2261 |
| BstZ17I | GTATAC | 1 cut(s) | 2517 |
| BsuI | GTATCC | 3 cut(s) | 113, 1363, 2626 |
| BtgI | CCRYGG | 1 cut(s) | 705 |
| BtgZI | GCGATG | 1 cut(s) | 1783 |
| BtrI | CACGTC | 1 cut(s) | 673 |
| BtsIMutI | CAGTG | 5 cut(s) | 917, 1624, 2136, 2301, 2617 |
| BveI | ACCTGC | 1 cut(s) | 883 |
| Cac8I | GCNNGC | 4 cut(s) | 238, 622, 976, 1766 |
| CaiI | CAGNNNCTG | 3 cut(s) | 595, 1862, 2668 |
| CfoI | GCGC | 1 cut(s) | 626 |
| Cfr10I | RCCGGY | 2 cut(s) | 1764, 1896 |
| Cfr42I | CCGCGG | 1 cut(s) | 708 |
| CseI | GACGC | 2 cut(s) | 838, 1424 |
| Csp6I | GTAC | 8 cut(s) | 115, 496, 541, 853, 1259, 1615, 2189, 2382 |
| CspAI | ACCGGT | 1 cut(s) | 1896 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 379, 414 |
| CviAII | CATG | 7 cut(s) | 667, 719, 748, 883, 1015, 2192, 2198 |
| CviQI | GTAC | 8 cut(s) | 115, 496, 541, 853, 1259, 1615, 2189, 2382 |
| DdeI | CTNAG | 2 cut(s) | 196, 260 |
| DraIII | CACNNNGTG | 1 cut(s) | 1271 |
| DriI | GACNNNNNGTC | 1 cut(s) | 676 |
| EaeI | YGGCCR | 2 cut(s) | 868, 1102 |
| Eam1104I | CTCTTC | 2 cut(s) | 1703, 2117 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 676 |
| EarI | CTCTTC | 2 cut(s) | 1703, 2117 |
| EciI | GGCGGA | 1 cut(s) | 1820 |
| Ecl136II | GAGCTC | 2 cut(s) | 862, 2418 |
| Eco130I | CCWWGG | 1 cut(s) | 2452 |
| Eco147I | AGGCCT | 2 cut(s) | 1887, 2231 |
| Eco24I | GRGCYC | 2 cut(s) | 864, 2420 |
| Eco31I | GGTCTC | 3 cut(s) | 1334, 1564, 1800 |
| Eco47I | GGWCC | 8 cut(s) | 206, 490, 531, 651, 824, 1126, 1947, 2607 |
| Eco53kI | GAGCTC | 2 cut(s) | 862, 2418 |
| Eco57I | CTGAAG | 3 cut(s) | 1773, 2004, 2672 |
| Eco88I | CYCGRG | 2 cut(s) | 820, 2483 |
| Eco91I | GGTNACC | 1 cut(s) | 678 |
| EcoICRI | GAGCTC | 2 cut(s) | 862, 2418 |
| EcoO109I | RGGNCCY | 4 cut(s) | 651, 1785, 1947, 1962 |
| EcoO65I | GGTNACC | 1 cut(s) | 678 |
| EcoRI | GAATTC | 1 cut(s) | 264 |
| EcoRII | CCWGG | 4 cut(s) | 1129, 1231, 1542, 1675 |
| EcoT14I | CCWWGG | 1 cut(s) | 2452 |
| EcoT22I | ATGCAT | 1 cut(s) | 2083 |
| EcoT38I | GRGCYC | 2 cut(s) | 864, 2420 |
| ErhI | CCWWGG | 1 cut(s) | 2452 |
| FaeI | CATG | 7 cut(s) | 670, 722, 751, 886, 1018, 2195, 2201 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2558, 2590 |
| FaqI | GGGAC | 2 cut(s) | 637, 1960 |
| FatI | CATG | 7 cut(s) | 666, 718, 747, 882, 1014, 2191, 2197 |
| FauI | CCCGC | 3 cut(s) | 422, 466, 700 |
| FbaI | TGATCA | 3 cut(s) | 183, 1551, 2176 |
| FblI | GTMKAC | 2 cut(s) | 2139, 2516 |
| Fnu4HI | GCNGC | 4 cut(s) | 1179, 1644, 1863, 2247 |
| FriOI | GRGCYC | 2 cut(s) | 864, 2420 |
| Fsp4HI | GCNGC | 4 cut(s) | 1179, 1644, 1863, 2247 |
| FspBI | CTAG | 8 cut(s) | 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683 |
| GlaI | GCGC | 1 cut(s) | 625 |
| GluI | GCNGC | 4 cut(s) | 1179, 1644, 1863, 2247 |
| GsuI | CTGGAG | 3 cut(s) | 1653, 2107, 2325 |
| HaeII | RGCGCY | 1 cut(s) | 627 |
| HgaI | GACGC | 2 cut(s) | 838, 1424 |
| HhaI | GCGC | 1 cut(s) | 626 |
| Hin1I | GRCGYC | 2 cut(s) | 432, 830 |
| Hin1II | CATG | 7 cut(s) | 670, 722, 751, 886, 1018, 2195, 2201 |
| Hin6I | GCGC | 1 cut(s) | 624 |
| HinP1I | GCGC | 1 cut(s) | 624 |
| HindIII | AAGCTT | 1 cut(s) | 227 |
| Hpy99I | CGWCG | 3 cut(s) | 434, 437, 832 |
| HpyCH4III | ACNGT | 6 cut(s) | 409, 684, 1456, 2016, 2479, 2582 |
| HpyCH4IV | ACGT | 4 cut(s) | 432, 672, 1198, 2159 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 617, 965, 1868, 2298 |
| HpyF3I | CTNAG | 2 cut(s) | 196, 260 |
| HpySE526I | ACGT | 4 cut(s) | 432, 672, 1198, 2159 |
| Hsp92I | GRCGYC | 2 cut(s) | 432, 830 |
| Hsp92II | CATG | 7 cut(s) | 670, 722, 751, 886, 1018, 2195, 2201 |
| HspAI | GCGC | 1 cut(s) | 624 |
| KflI | GGGWCCC | 2 cut(s) | 651, 1947 |
| Kpn2I | TCCGGA | 2 cut(s) | 1424, 2144 |
| KroI | GCCGGC | 1 cut(s) | 1764 |
| KroNI | GCCGGC | 1 cut(s) | 1766 |
| Ksp22I | TGATCA | 3 cut(s) | 183, 1551, 2176 |
| KspI | CCGCGG | 1 cut(s) | 708 |
| LmnI | GCTCC | 9 cut(s) | 197, 582, 847, 859, 1718, 1908, 2063, 2537, 2647 |
| Lsp1109I | GCAGC | 4 cut(s) | 1190, 1655, 1849, 2258 |
| LweI | GCATC | 7 cut(s) | 987, 1009, 1059, 1472, 1874, 2013, 2155 |
| MaeI | CTAG | 8 cut(s) | 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683 |
| MaeII | ACGT | 4 cut(s) | 432, 672, 1198, 2159 |
| MhlI | GDGCHC | 5 cut(s) | 864, 1119, 2303, 2420, 2528 |
| MlsI | TGGCCA | 1 cut(s) | 1104 |
| MluNI | TGGCCA | 1 cut(s) | 1104 |
| MlyI | GAGTC | 6 cut(s) | 140, 272, 322, 1437, 1689, 2135 |
| MmeI | TCCRAC | 8 cut(s) | 450, 604, 851, 1696, 2142, 2438, 2469, 2625 |
| Mox20I | TGGCCA | 1 cut(s) | 1104 |
| Mph1103I | ATGCAT | 1 cut(s) | 2083 |
| MroI | TCCGGA | 2 cut(s) | 1424, 2144 |
| MroNI | GCCGGC | 1 cut(s) | 1764 |
| MscI | TGGCCA | 1 cut(s) | 1104 |
| MseI | TTAA | 4 cut(s) | 2076, 2447, 2499, 2565 |
| MslI | CAYNNNNRTG | 6 cut(s) | 671, 1098, 1922, 2184, 2196, 2583 |
| Msp20I | TGGCCA | 1 cut(s) | 1104 |
| MspA1I | CMGCKG | 1 cut(s) | 707 |
| MspR9I | CCNGG | 8 cut(s) | 154, 521, 529, 1131, 1217, 1233, 1544, 1677 |
| Mva1269I | GAATGC | 1 cut(s) | 2261 |
| MvaI | CCWGG | 4 cut(s) | 1131, 1233, 1544, 1677 |
| MvnI | CGCG | 1 cut(s) | 707 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 617, 965, 1868, 2298 |
| NaeI | GCCGGC | 1 cut(s) | 1766 |
| NciI | CCSGG | 4 cut(s) | 154, 521, 529, 1217 |
| NgoMIV | GCCGGC | 1 cut(s) | 1764 |
| NheI | GCTAGC | 1 cut(s) | 974 |
| NlaIII | CATG | 7 cut(s) | 670, 722, 751, 886, 1018, 2195, 2201 |
| NmuCI | GTSAC | 6 cut(s) | 409, 678, 689, 767, 1655, 2043 |
| NsiI | ATGCAT | 1 cut(s) | 2083 |
| NspI | RCATGY | 1 cut(s) | 2195 |
| NspV | TTCGAA | 2 cut(s) | 268, 2265 |
| PceI | AGGCCT | 2 cut(s) | 1887, 2231 |
| PciI | ACATGT | 1 cut(s) | 2191 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 438 |
| PctI | GAATGC | 1 cut(s) | 2261 |
| PdiI | GCCGGC | 1 cut(s) | 1766 |
| PfeI | GAWTC | 7 cut(s) | 146, 903, 1421, 1526, 1774, 1853, 2659 |
| PfoI | TCCNGGA | 2 cut(s) | 152, 519 |
| PinAI | ACCGGT | 1 cut(s) | 1896 |
| PkrI | GCNGC | 4 cut(s) | 1180, 1645, 1864, 2248 |
| PleI | GAGTC | 6 cut(s) | 139, 272, 322, 1436, 1689, 2135 |
| PpsI | GAGTC | 6 cut(s) | 139, 272, 322, 1436, 1689, 2135 |
| PpuMI | RGGWCCY | 2 cut(s) | 651, 1947 |
| PscI | ACATGT | 1 cut(s) | 2191 |
| PsiI | TTATAA | 2 cut(s) | 783, 1364 |
| Psp124BI | GAGCTC | 2 cut(s) | 864, 2420 |
| Psp5II | RGGWCCY | 2 cut(s) | 651, 1947 |
| Psp6I | CCWGG | 4 cut(s) | 1129, 1231, 1542, 1675 |
| PspEI | GGTNACC | 1 cut(s) | 678 |
| PspGI | CCWGG | 4 cut(s) | 1129, 1231, 1542, 1675 |
| PspPPI | RGGWCCY | 2 cut(s) | 651, 1947 |
| PstNI | CAGNNNCTG | 3 cut(s) | 595, 1862, 2668 |
| RsaI | GTAC | 8 cut(s) | 116, 497, 542, 854, 1260, 1616, 2190, 2383 |
| RsaNI | GTAC | 8 cut(s) | 115, 496, 541, 853, 1259, 1615, 2189, 2382 |
| RseI | CAYNNNNRTG | 6 cut(s) | 671, 1098, 1922, 2184, 2196, 2583 |
| SacI | GAGCTC | 2 cut(s) | 864, 2420 |
| SacII | CCGCGG | 1 cut(s) | 708 |
| SaqAI | TTAA | 4 cut(s) | 2076, 2447, 2499, 2565 |
| SatI | GCNGC | 4 cut(s) | 1179, 1644, 1863, 2247 |
| SchI | GAGTC | 6 cut(s) | 140, 272, 322, 1437, 1689, 2135 |
| ScrFI | CCNGG | 8 cut(s) | 154, 521, 529, 1131, 1217, 1233, 1544, 1677 |
| SduI | GDGCHC | 5 cut(s) | 864, 1119, 2303, 2420, 2528 |
| SfaNI | GCATC | 7 cut(s) | 987, 1009, 1059, 1472, 1874, 2013, 2155 |
| SfcI | CTRYAG | 1 cut(s) | 2625 |
| Sfr303I | CCGCGG | 1 cut(s) | 708 |
| SfuI | TTCGAA | 2 cut(s) | 268, 2265 |
| SgrBI | CCGCGG | 1 cut(s) | 708 |
| SinI | GGWCC | 8 cut(s) | 206, 490, 531, 651, 824, 1126, 1947, 2607 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 671, 1098, 1922, 2184, 2196, 2583 |
| SmlI | CTYRAG | 1 cut(s) | 224 |
| SmoI | CTYRAG | 1 cut(s) | 224 |
| SseBI | AGGCCT | 2 cut(s) | 1887, 2231 |
| SsiI | CCGC | 7 cut(s) | 415, 459, 705, 707, 1831, 2171, 2292 |
| SspI | AATATT | 1 cut(s) | 39 |
| SspMI | CTAG | 8 cut(s) | 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683 |
| SstI | GAGCTC | 2 cut(s) | 864, 2420 |
| StuI | AGGCCT | 2 cut(s) | 1887, 2231 |
| StyD4I | CCNGG | 8 cut(s) | 152, 519, 527, 1129, 1215, 1231, 1542, 1675 |
| StyI | CCWWGG | 1 cut(s) | 2452 |
| TaaI | ACNGT | 6 cut(s) | 409, 684, 1456, 2016, 2479, 2582 |
| TaiI | ACGT | 4 cut(s) | 435, 675, 1201, 2162 |
| TaqI | TCGA | 7 cut(s) | 129, 268, 441, 633, 2265, 2337, 2427 |
| TatI | WGTACW | 5 cut(s) | 495, 852, 1258, 1614, 2188 |
| TfiI | GAWTC | 7 cut(s) | 146, 903, 1421, 1526, 1774, 1853, 2659 |
| Tru1I | TTAA | 4 cut(s) | 2076, 2447, 2499, 2565 |
| Tru9I | TTAA | 4 cut(s) | 2076, 2447, 2499, 2565 |
| TscAI | CASTG | 5 cut(s) | 917, 1631, 2136, 2308, 2617 |
| TseFI | GTSAC | 6 cut(s) | 409, 678, 689, 767, 1655, 2043 |
| TseI | GCWGC | 4 cut(s) | 1178, 1643, 1862, 2246 |
| Tsp45I | GTSAC | 6 cut(s) | 409, 678, 689, 767, 1655, 2043 |
| TspDTI | ATGAA | 4 cut(s) | 258, 1358, 1493, 2214 |
| TspGWI | ACGGA | 5 cut(s) | 132, 290, 708, 872, 1880 |
| TspRI | CASTG | 5 cut(s) | 917, 1631, 2136, 2308, 2617 |
| VpaK11BI | GGWCC | 8 cut(s) | 206, 490, 531, 651, 824, 1126, 1947, 2607 |
| XapI | RAATTY | 4 cut(s) | 264, 1687, 2068, 2438 |
| XceI | RCATGY | 1 cut(s) | 2195 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 917 |
| XmiI | GTMKAC | 2 cut(s) | 2139, 2516 |
| XspI | CTAG | 8 cut(s) | 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683 |
| ZraI | GACGTC | 1 cut(s) | 433 |
| Zsp2I | ATGCAT | 1 cut(s) | 2083 |