Rroxscaffold_1G00057780

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000882
Physical Location & Seq
Forward (+)
79772987 .. 79776508
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00057780.1

Sequence Viewer

Length: 2388 bp
ATGGAAAGAAACCGATCAAAATACACACCAAAAGCCACATTCCATCATCTTCTACCATTCTTCTTCTATATCTTCTTCTCTCTTGATTGCCTAGTTGCACAAGAGTTAGTTGGCTATGAGACCAAAGACCGTCTAAGAGTTCGAATAACAGACTCAACGCAACAAAGATGGGAGCTCCCACAAGACATTCTCCCCCGCCAAAACCAAACTTCACAGAACTTTTCCGTCACTGACAACGACCTCGTTTTCACCCTCCGCAACACCACCCCGTTCGGCTTCACCATCACCCGCCGCTCCTCTAACGACACCGTTTTCGACACCACCCCAAACCCCTCCGACCCCAACACCACCTTCATTTTTAAGGACCAATACATCCAGCTCTCCTCCTCTCTCCCGAACAACAGGTCCTCCCTGTACGGCCTCGGAGAGCATACCAAGAGCACCTTAAAGCTCCTAGCCAATCAGACTCTGACACTCTGGACCGCCGACATTGGCAGCGTGAATCCCGACCTCAATCTCTATGGGTCCCACCCCTTCTACATGGACGTAAGGTCGCCGTCCGGTGATGGCAAGGTCAAGGCGGGAACCACTCACGGAGTCCTGCTTTTGAACAGCAATGGCATGGATGTAAATTACACCGGTGACAGGATTACTTATAATGTGATTGGCGGAGTTGTGGACTTGTATTTCTTTTCGGGATTCCACCAGTGTAGATATGGTTACAAGAATGTTTCTGATCTTGAGGGGGTCGTTGCTGGTTATGCAAATGCTAGTATCCCTCTTGAGGTTATGTGGACAGACATTGATTACATGGATGCTTACAAGGATTTCACTCTTGATCCCGTCAACTTTCCATTAGACAGGATGAAGAACTTTACCAACAACCTTCACCAAAATGGTCAGAAATACGTGCTCATCTTGGATCCCGGTATTAGTATCAATGATAGCTATGGAGCCTACACCAGAGGGAAGGAAGCAGATATATTTATAAAACGTGATGGCATTCCTTACCAGGGAAATGTTTGGCCTGGCAATGTCTACTTTCCTGATTTTGTACATCCACAAAGTGAACCATATTGGGAGAATGAGATTAAACTATTTCTAGACCAGCTACCTGTTGATGGCCTTTGGCTTGATATGAATGAAGTATCAAATTTCCAAACTTCCAATCCCACTCCGAATTCCACACTTGATGATCCACCTTACAAAATCAACGATGCTGGAGGCCACCGTCCGATCCTTAGTAGGACTGTTCCAGGATCAGCTCTGCACTTTGGTAATGTTACAGAGTATAATGTTCATAACCTATTTGGCATGTTACAAGCCAAAGCAACAAACAAGGCCTTGACCAATTTGACTGGTAAGAGACCTTTTGTTCTCTCGAGATCGACTTTTGTAAGCTCTGGTAAGTACACAGCACACTGGACCGGAGACAATGGTGCAAAATGGAGTGATTTGGCATACTCAATTCCGGGCATTTTGAATTTTGGACTCTTTGGAGTTCCAATGGTTGGAGCTGATATATGTGGTTTCTCTGGGGATACTAATGAAGAACTCTGCCGGCGTTGGATTCAGCTAGGGGCCTTTTACCCCTTTTCAAGAGATCATTCGGACAAGAATTCAATCCGGCAGGAGCTCTATGTTTGGGAATCAGTGGCTGCATCAGCAAAAAAAGTGCTGGGGCTACGTTACCGGTTGCTTCCCTTGTTCTATACATCAATGTATCAAGCACACAAGAACGGGACTCCAATTGCAAGGCCCGTATTCTTCTCATTTCCTGAAGATACCAATACATACAGTATCTATTCACAGTTTCTGATTGGTAAAGGCGTAATGGTGTCACCTGTGTTGCAGCATGGAGCAAATTCAGTTGAGGCATATTTTCCTGCAGGAAATTGGTTCGATCTCTTCAACTACACATCAGTTAGTGTAGACTCTGGAAAGAATGTTACGTTGGATGCACCGCCTGATCATATCAATGTGCATGTCCACGAAGGAAACATTTTGGCCTTGCAAGGAGAGGCCTTGACTACACAAGCAGCCCGAAACACTACATTCGAACTTTTGGTGGTCATTGGTAGTAGTAATGAGAGTACCGGAGAAGTGTTCTTGGACAATGGAGAGGATGTTGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTTGTGAGGTTTCATTCTGCACGAGCACAAAATGGAAGTCTAGTTTTGAGCTCAAAGGTCGAAAATGGAGAATTTGCATTAAGCCAAGGTTGGATCATTGATAAAATTACCGTTCTTGGGTTGGACAAAGTTAATGCAACAGAGTCCAGTGTATCCCTTCCTATAGGAAAGGAGTTTGAGTTGGAGCTGAAGCCGAATCAAACACTGGCGAAAACACTCTTTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

795

Amino Acids

88.89

Weight (kDa)

5.54

Isoelectric Point (pI)

29.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 45 - 205 2.1e-11 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 230 - 572 6e-118 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 581 - 669 4.9e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 657, 989
AccB7I CCANNNNNTGG 1 cut(s) 1511
AccBSI CCGCTC 1 cut(s) 294
AccI GTMKAC 2 cut(s) 1038, 1932
AciI CCGC 8 cut(s) 196, 256, 289, 292, 483, 581, 669, 1964
AclWI GGATC 7 cut(s) 833, 917, 930, 1190, 1231, 1267, 2261
AcsI RAATTY 6 cut(s) 1153, 1180, 1483, 1618, 1864, 2231
AcuI CTGAAG 2 cut(s) 1800, 2369
AdeI CACNNNGTG 1 cut(s) 1067
AfaI GTAC 4 cut(s) 416, 1056, 1412, 2095
AfiI CCNNNNNNNGG 6 cut(s) 645, 784, 1013, 1121, 1511, 2277
AgeI ACCGGT 2 cut(s) 638, 1692
AgsI TTSAA 6 cut(s) 610, 1483, 1599, 1623, 1912, 2132
AhdI GACNNNNNGTC 1 cut(s) 550
AjnI CCWGG 3 cut(s) 1011, 1027, 1255
AjuI GAANNNNNNNTTGG 4 cut(s) 335, 367, 1937, 1969
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
Alw21I GWGCWC 6 cut(s) 177, 443, 915, 1638, 2188, 2213
Alw26I GTCTC 3 cut(s) 113, 1360, 1425
AlwI GGATC 7 cut(s) 833, 917, 930, 1190, 1231, 1267, 2261
AlwNI CAGNNNCTG 3 cut(s) 469, 1658, 1816
Ama87I CYCGRG 1 cut(s) 1381
AoxI GGCC 9 cut(s) 418, 1025, 1123, 1225, 1341, 1581, 1757, 2007, 2022
ApeKI GCWGC 4 cut(s) 495, 1658, 1852, 2039
ApoI RAATTY 6 cut(s) 1153, 1180, 1483, 1618, 1864, 2231
ArsI GACNNNNNNTTYG 6 cut(s) 296, 328, 389, 421, 2182, 2214
AsiGI ACCGGT 2 cut(s) 638, 1692
AspS9I GGNCC 7 cut(s) 364, 405, 480, 525, 1425, 1581, 1758
AsuC2I CCSGG 2 cut(s) 927, 1473
AsuHPI GGTGA 7 cut(s) 241, 271, 277, 575, 653, 881, 1833
AsuII TTCGAA 2 cut(s) 142, 2058
AvaI CYCGRG 1 cut(s) 1381
AvaII GGWCC 5 cut(s) 364, 405, 480, 525, 1425
BamHI GGATCC 1 cut(s) 922
BanII GRGCYC 3 cut(s) 177, 1638, 2213
BauI CACGAG 1 cut(s) 2181
Bbv12I GWGCWC 6 cut(s) 177, 443, 915, 1638, 2188, 2213
BbvI GCAGC 4 cut(s) 507, 1645, 1864, 2051
BccI CCATC 6 cut(s) 51, 162, 290, 560, 992, 1115
BceAI ACGGC 2 cut(s) 433, 541
BciT130I CCWGG 3 cut(s) 1013, 1029, 1257
BciVI GTATCC 3 cut(s) 785, 1534, 2323
BclI TGATCA 1 cut(s) 1969
BcnI CCSGG 2 cut(s) 927, 1473
BcoDI GTCTC 3 cut(s) 113, 1360, 1425
BfaI CTAG 7 cut(s) 92, 455, 771, 1103, 1577, 2201, 2386
BfmI CTRYAG 2 cut(s) 1887, 2322
BfuI GTATCC 3 cut(s) 785, 1534, 2323
BisI GCNGC 5 cut(s) 292, 496, 1659, 1853, 2040
BlsI GCNGC 5 cut(s) 293, 497, 1660, 1854, 2041
Bme1390I CCNGG 5 cut(s) 927, 1013, 1029, 1257, 1473
Bme18I GGWCC 5 cut(s) 364, 405, 480, 525, 1425
BmeRI GACNNNNNGTC 1 cut(s) 550
BmeT110I CYCGRG 1 cut(s) 1381
BmgT120I GGNCC 7 cut(s) 364, 405, 480, 525, 1425, 1581, 1758
BmiI GGNNCC 6 cut(s) 526, 527, 586, 924, 955, 1582
BmrFI CCNGG 5 cut(s) 927, 1013, 1029, 1257, 1473
BmsI GCATC 4 cut(s) 805, 1207, 1670, 1948
BpmI CTGGAG 1 cut(s) 1242
Bpu14I TTCGAA 2 cut(s) 142, 2058
BpuEI CTTGAG 2 cut(s) 761, 803
BpuMI CCSGG 2 cut(s) 927, 1473
BsaAI YACGTR 1 cut(s) 910
BsaI GGTCTC 2 cut(s) 113, 1360
BsaJI CCNNGG 3 cut(s) 421, 1012, 2245
BsaWI WCCGGW 5 cut(s) 560, 638, 1427, 1692, 2096
BsaXI ACNNNNNCTCC 6 cut(s) 392, 422, 2112, 2142, 2324, 2354
Bsc4I CCNNNNNNNGG 6 cut(s) 645, 784, 1013, 1121, 1511, 2277
Bse118I RCCGGY 3 cut(s) 638, 1560, 1692
Bse1I ACTGG 5 cut(s) 706, 1363, 1427, 2307, 2370
Bse3DI GCAATG 2 cut(s) 622, 1039
BseBI CCWGG 3 cut(s) 1013, 1029, 1257
BseDI CCNNGG 3 cut(s) 421, 1012, 2245
BseGI GGATG 7 cut(s) 372, 631, 820, 870, 1057, 1963, 2131
BseLI CCNNNNNNNGG 6 cut(s) 645, 784, 1013, 1121, 1511, 2277
BseMI GCAATG 2 cut(s) 622, 1039
BseNI ACTGG 5 cut(s) 706, 1363, 1427, 2307, 2370
BseRI GAGGAG 4 cut(s) 286, 373, 376, 2154
BseXI GCAGC 4 cut(s) 507, 1645, 1864, 2051
BseYI CCCAGC 1 cut(s) 1678
BsgI GTGCAG 2 cut(s) 1253, 2163
BshFI GGCC 9 cut(s) 420, 1027, 1125, 1227, 1343, 1583, 1759, 2009, 2024
BshTI ACCGGT 2 cut(s) 638, 1692
BsiHKAI GWGCWC 6 cut(s) 177, 443, 915, 1638, 2188, 2213
BsiHKCI CYCGRG 1 cut(s) 1381
BsiSI CCGG 9 cut(s) 561, 639, 927, 1428, 1472, 1561, 1627, 1693, 2097
BslFI GGGAC 2 cut(s) 511, 1756
BslI CCNNNNNNNGG 6 cut(s) 645, 784, 1013, 1121, 1511, 2277
BsmAI GTCTC 3 cut(s) 113, 1360, 1425
BsmFI GGGAC 2 cut(s) 511, 1756
BsmI GAATGC 1 cut(s) 1002
BsnI GGCC 9 cut(s) 420, 1027, 1125, 1227, 1343, 1583, 1759, 2009, 2024
Bso31I GGTCTC 2 cut(s) 113, 1360
BsoBI CYCGRG 1 cut(s) 1381
Bsp119I TTCGAA 2 cut(s) 142, 2058
Bsp1286I GDGCHC 6 cut(s) 177, 443, 915, 1638, 2188, 2213
Bsp1407I TGTACA 1 cut(s) 1054
BspACI CCGC 8 cut(s) 196, 256, 289, 292, 483, 581, 669, 1964
BspANI GGCC 9 cut(s) 420, 1027, 1125, 1227, 1343, 1583, 1759, 2009, 2024
BspLI GGNNCC 6 cut(s) 526, 527, 586, 924, 955, 1582
BspMAI CTGCAG 1 cut(s) 1891
BspPI GGATC 7 cut(s) 833, 917, 930, 1190, 1231, 1267, 2261
BspT104I TTCGAA 2 cut(s) 142, 2058
BspTNI GGTCTC 2 cut(s) 113, 1360
BsrBI CCGCTC 1 cut(s) 294
BsrDI GCAATG 2 cut(s) 622, 1039
BsrFI RCCGGY 3 cut(s) 638, 1560, 1692
BsrGI TGTACA 1 cut(s) 1054
BsrI ACTGG 5 cut(s) 706, 1363, 1427, 2307, 2370
BssAI RCCGGY 3 cut(s) 638, 1560, 1692
BssECI CCNNGG 3 cut(s) 421, 1012, 2245
BssSI CACGAG 1 cut(s) 2181
BssT1I CCWWGG 1 cut(s) 2245
Bst2BI CACGAG 1 cut(s) 2181
Bst2UI CCWGG 3 cut(s) 1013, 1029, 1257
Bst4CI ACNGT 7 cut(s) 131, 310, 1232, 1252, 1799, 1812, 2272
Bst6I CTCTTC 1 cut(s) 1913
BstAUI TGTACA 1 cut(s) 1054
BstBAI YACGTR 1 cut(s) 910
BstBI TTCGAA 2 cut(s) 142, 2058
BstC8I GCNNGC 1 cut(s) 1562
BstDEI CTNAG 2 cut(s) 134, 1241
BstF5I GGATG 7 cut(s) 372, 631, 820, 870, 1057, 1963, 2131
BstMAI GTCTC 3 cut(s) 113, 1360, 1425
BstMWI GCNNNNNNNGC 2 cut(s) 761, 1664
BstNI CCWGG 3 cut(s) 1013, 1029, 1257
BstNSI RCATGY 2 cut(s) 1318, 1988
BstSCI CCNGG 5 cut(s) 925, 1011, 1027, 1255, 1471
BstSFI CTRYAG 2 cut(s) 1887, 2322
BstV1I GCAGC 4 cut(s) 507, 1645, 1864, 2051
BstX2I RGATCY 1 cut(s) 922
BstYI RGATCY 1 cut(s) 922
BsuI GTATCC 3 cut(s) 785, 1534, 2323
BsuRI GGCC 9 cut(s) 420, 1027, 1125, 1227, 1343, 1583, 1759, 2009, 2024
BtsCI GGATG 7 cut(s) 372, 631, 820, 870, 1057, 1963, 2131
BtsIMutI CAGTG 6 cut(s) 228, 713, 1420, 1659, 2314, 2363
Cac8I GCNNGC 1 cut(s) 1562
CaiI CAGNNNCTG 3 cut(s) 469, 1658, 1816
Cfr10I RCCGGY 3 cut(s) 638, 1560, 1692
Cfr13I GGNCC 7 cut(s) 364, 405, 480, 525, 1425, 1581, 1758
Csp6I GTAC 4 cut(s) 415, 1055, 1411, 2094
CspAI ACCGGT 2 cut(s) 638, 1692
CviAII CATG 6 cut(s) 541, 622, 811, 1315, 1856, 1985
CviQI GTAC 4 cut(s) 415, 1055, 1411, 2094
DdeI CTNAG 2 cut(s) 134, 1241
DraIII CACNNNGTG 1 cut(s) 1067
DriI GACNNNNNGTC 1 cut(s) 550
Eam1104I CTCTTC 1 cut(s) 1913
Eam1105I GACNNNNNGTC 1 cut(s) 550
EarI CTCTTC 1 cut(s) 1913
EciI GGCGGA 1 cut(s) 684
Ecl136II GAGCTC 3 cut(s) 175, 1636, 2211
Eco130I CCWWGG 1 cut(s) 2245
Eco147I AGGCCT 2 cut(s) 1343, 2024
Eco24I GRGCYC 3 cut(s) 177, 1638, 2213
Eco31I GGTCTC 2 cut(s) 113, 1360
Eco47I GGWCC 5 cut(s) 364, 405, 480, 525, 1425
Eco53kI GAGCTC 3 cut(s) 175, 1636, 2211
Eco57I CTGAAG 2 cut(s) 1800, 2369
Eco88I CYCGRG 1 cut(s) 1381
EcoICRI GAGCTC 3 cut(s) 175, 1636, 2211
EcoO109I RGGNCCY 3 cut(s) 405, 525, 1581
EcoRI GAATTC 2 cut(s) 1180, 1618
EcoRII CCWGG 3 cut(s) 1011, 1027, 1255
EcoT14I CCWWGG 1 cut(s) 2245
EcoT38I GRGCYC 3 cut(s) 177, 1638, 2213
ErhI CCWWGG 1 cut(s) 2245
FaeI CATG 6 cut(s) 544, 625, 814, 1318, 1859, 1988
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FaqI GGGAC 2 cut(s) 511, 1756
FatI CATG 6 cut(s) 540, 621, 810, 1314, 1855, 1984
FauI CCCGC 3 cut(s) 203, 296, 574
FbaI TGATCA 1 cut(s) 1969
FblI GTMKAC 2 cut(s) 1038, 1932
Fnu4HI GCNGC 5 cut(s) 292, 496, 1659, 1853, 2040
FokI GGATG 7 cut(s) 359, 638, 827, 877, 1044, 1970, 2138
FriOI GRGCYC 3 cut(s) 177, 1638, 2213
Fsp4HI GCNGC 5 cut(s) 292, 496, 1659, 1853, 2040
FspBI CTAG 7 cut(s) 92, 455, 771, 1103, 1577, 2201, 2386
GluI GCNGC 5 cut(s) 292, 496, 1659, 1853, 2040
GsaI CCCAGC 1 cut(s) 1682
GsuI CTGGAG 1 cut(s) 1242
HaeIII GGCC 9 cut(s) 420, 1027, 1125, 1227, 1343, 1583, 1759, 2009, 2024
HapII CCGG 9 cut(s) 561, 639, 927, 1428, 1472, 1561, 1627, 1693, 2097
Hin1II CATG 6 cut(s) 544, 625, 814, 1318, 1859, 1988
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HpaII CCGG 9 cut(s) 561, 639, 927, 1428, 1472, 1561, 1627, 1693, 2097
HphI GGTGA 7 cut(s) 241, 271, 277, 575, 653, 881, 1833
Hpy166II GTNNAC 8 cut(s) 679, 795, 847, 1039, 1070, 1413, 1933, 1990
Hpy8I GTNNAC 8 cut(s) 679, 795, 847, 1039, 1070, 1413, 1933, 1990
HpyAV CCTTC 6 cut(s) 361, 544, 896, 964, 1988, 2327
HpyCH4III ACNGT 7 cut(s) 131, 310, 1232, 1252, 1799, 1812, 2272
HpyCH4IV ACGT 5 cut(s) 546, 909, 994, 1687, 1952
HpyF10VI GCNNNNNNNGC 2 cut(s) 761, 1664
HpyF3I CTNAG 2 cut(s) 134, 1241
HpySE526I ACGT 5 cut(s) 546, 909, 994, 1687, 1952
Hsp92II CATG 6 cut(s) 544, 625, 814, 1318, 1859, 1988
KflI GGGWCCC 1 cut(s) 525
KroI GCCGGC 1 cut(s) 1560
KroNI GCCGGC 1 cut(s) 1562
Ksp22I TGATCA 1 cut(s) 1969
LmnI GCTCC 9 cut(s) 172, 180, 299, 456, 953, 1514, 1633, 1859, 2344
Lsp1109I GCAGC 4 cut(s) 507, 1645, 1864, 2051
LweI GCATC 4 cut(s) 805, 1207, 1670, 1948
MaeI CTAG 7 cut(s) 92, 455, 771, 1103, 1577, 2201, 2386
MaeII ACGT 5 cut(s) 546, 909, 994, 1687, 1952
MaeIII GTNAC 8 cut(s) 226, 641, 719, 1282, 1317, 1688, 1839, 1948
MbiI CCGCTC 1 cut(s) 294
MfeI CAATTG 1 cut(s) 1749
MflI RGATCY 1 cut(s) 922
MhlI GDGCHC 6 cut(s) 177, 443, 915, 1638, 2188, 2213
MlyI GAGTC 7 cut(s) 146, 460, 606, 1485, 1738, 1928, 2312
MmeI TCCRAC 7 cut(s) 360, 1492, 1547, 1935, 2231, 2262, 2322
MroNI GCCGGC 1 cut(s) 1560
MseI TTAA 5 cut(s) 360, 446, 1092, 2240, 2292
MslI CAYNNNNRTG 2 cut(s) 894, 1977
MspI CCGG 9 cut(s) 561, 639, 927, 1428, 1472, 1561, 1627, 1693, 2097
MspR9I CCNGG 5 cut(s) 927, 1013, 1029, 1257, 1473
MunI CAATTG 1 cut(s) 1749
Mva1269I GAATGC 1 cut(s) 1002
MvaI CCWGG 3 cut(s) 1013, 1029, 1257
MwoI GCNNNNNNNGC 2 cut(s) 761, 1664
NaeI GCCGGC 1 cut(s) 1562
NciI CCSGG 2 cut(s) 927, 1473
NgoMIV GCCGGC 1 cut(s) 1560
NlaIII CATG 6 cut(s) 544, 625, 814, 1318, 1859, 1988
NlaIV GGNNCC 6 cut(s) 526, 527, 586, 924, 955, 1582
NmuCI GTSAC 3 cut(s) 226, 641, 1839
NspI RCATGY 2 cut(s) 1318, 1988
NspV TTCGAA 2 cut(s) 142, 2058
PaeR7I CTCGAG 1 cut(s) 1381
PceI AGGCCT 2 cut(s) 1343, 2024
PctI GAATGC 1 cut(s) 1002
PdiI GCCGGC 1 cut(s) 1562
PfeI GAWTC 5 cut(s) 502, 699, 1570, 1649, 2356
PflMI CCANNNNNTGG 1 cut(s) 1511
PfoI TCCNGGA 1 cut(s) 1255
PinAI ACCGGT 2 cut(s) 638, 1692
PkrI GCNGC 5 cut(s) 293, 497, 1660, 1854, 2041
PleI GAGTC 7 cut(s) 146, 460, 605, 1485, 1738, 1928, 2311
PpsI GAGTC 7 cut(s) 146, 460, 605, 1485, 1738, 1928, 2311
Ppu21I YACGTR 1 cut(s) 910
PpuMI RGGWCCY 2 cut(s) 405, 525
PsiI TTATAA 2 cut(s) 657, 989
Psp124BI GAGCTC 3 cut(s) 177, 1638, 2213
Psp5II RGGWCCY 2 cut(s) 405, 525
Psp6I CCWGG 3 cut(s) 1011, 1027, 1255
PspFI CCCAGC 1 cut(s) 1678
PspGI CCWGG 3 cut(s) 1011, 1027, 1255
PspN4I GGNNCC 6 cut(s) 526, 527, 586, 924, 955, 1582
PspPI GGNCC 7 cut(s) 364, 405, 480, 525, 1425, 1581, 1758
PspPPI RGGWCCY 2 cut(s) 405, 525
PstI CTGCAG 1 cut(s) 1891
PstNI CAGNNNCTG 3 cut(s) 469, 1658, 1816
PsuI RGATCY 1 cut(s) 922
RsaI GTAC 4 cut(s) 416, 1056, 1412, 2095
RsaNI GTAC 4 cut(s) 415, 1055, 1411, 2094
RseI CAYNNNNRTG 2 cut(s) 894, 1977
SacI GAGCTC 3 cut(s) 177, 1638, 2213
SaqAI TTAA 5 cut(s) 360, 446, 1092, 2240, 2292
SatI GCNGC 5 cut(s) 292, 496, 1659, 1853, 2040
Sau96I GGNCC 7 cut(s) 364, 405, 480, 525, 1425, 1581, 1758
SbfI CCTGCAGG 1 cut(s) 1891
SchI GAGTC 7 cut(s) 146, 460, 606, 1485, 1738, 1928, 2312
ScrFI CCNGG 5 cut(s) 927, 1013, 1029, 1257, 1473
SdaI CCTGCAGG 1 cut(s) 1891
SduI GDGCHC 6 cut(s) 177, 443, 915, 1638, 2188, 2213
SfaNI GCATC 4 cut(s) 805, 1207, 1670, 1948
SfcI CTRYAG 2 cut(s) 1887, 2322
Sfr274I CTCGAG 1 cut(s) 1381
SfuI TTCGAA 2 cut(s) 142, 2058
SgrAI CRCCGGYG 1 cut(s) 638
SinI GGWCC 5 cut(s) 364, 405, 480, 525, 1425
SlaI CTCGAG 1 cut(s) 1381
SmiMI CAYNNNNRTG 2 cut(s) 894, 1977
SmlI CTYRAG 3 cut(s) 740, 782, 1381
SmoI CTYRAG 3 cut(s) 740, 782, 1381
Sse8387I CCTGCAGG 1 cut(s) 1891
SseBI AGGCCT 2 cut(s) 1343, 2024
SsiI CCGC 8 cut(s) 196, 256, 289, 292, 483, 581, 669, 1964
SspMI CTAG 7 cut(s) 92, 455, 771, 1103, 1577, 2201, 2386
SstI GAGCTC 3 cut(s) 177, 1638, 2213
StuI AGGCCT 2 cut(s) 1343, 2024
StyD4I CCNGG 5 cut(s) 925, 1011, 1027, 1255, 1471
StyI CCWWGG 1 cut(s) 2245
TaaI ACNGT 7 cut(s) 131, 310, 1232, 1252, 1799, 1812, 2272
TaiI ACGT 5 cut(s) 549, 912, 997, 1690, 1955
TaqI TCGA 7 cut(s) 142, 315, 1382, 1388, 1902, 2058, 2220
TatI WGTACW 2 cut(s) 1054, 1410
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 5 cut(s) 502, 699, 1570, 1649, 2356
Tru1I TTAA 5 cut(s) 360, 446, 1092, 2240, 2292
Tru9I TTAA 5 cut(s) 360, 446, 1092, 2240, 2292
TscAI CASTG 6 cut(s) 235, 713, 1427, 1659, 2314, 2370
TseFI GTSAC 3 cut(s) 226, 641, 1839
TseI GCWGC 4 cut(s) 495, 1658, 1852, 2039
Tsp45I GTSAC 3 cut(s) 226, 641, 1839
TspDTI ATGAA 7 cut(s) 343, 881, 1154, 1158, 1289, 1563, 2162
TspGWI ACGGA 2 cut(s) 214, 609
TspRI CASTG 6 cut(s) 235, 713, 1427, 1659, 2314, 2370
Van91I CCANNNNNTGG 1 cut(s) 1511
VpaK11BI GGWCC 5 cut(s) 364, 405, 480, 525, 1425
XapI RAATTY 6 cut(s) 1153, 1180, 1483, 1618, 1864, 2231
XbaI TCTAGA 1 cut(s) 1102
XceI RCATGY 2 cut(s) 1318, 1988
XcmI CCANNNNNNNNNTGG 1 cut(s) 713
XhoI CTCGAG 1 cut(s) 1381
XmiI GTMKAC 2 cut(s) 1038, 1932
XspI CTAG 7 cut(s) 92, 455, 771, 1103, 1577, 2201, 2386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.