Prupe.4G103600_v2.0.a1

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000882
Physical Location & Seq
Forward (+)
5302424 .. 5306500
4077 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G103600.2

Sequence Viewer

Length: 2730 bp
ATGAAGATGCAAAGAAGCAGACCAAACAGACCAAAACCAATATATCATCTTCCACTCTTCTTCTTCTTCTTCTTCTTCTTGTCTGCTGTCTTTCACTGTGGTTTAGTTTCATCATCAAATACCAAGGGAGCAAAAGAAGCTGCAGTTGCTGGGTTTGGATACAAAATTCAGTCAGTCAACTATGATTCTTCTGGGAACTCATTGACTGCTAATCTTGGCCTCATCAAGAAATCCTCCCTCTACGGACCCGACATCCCAAATCTCAACCTTCGCGCCAGCTATGAAACCAAAGATCGGCTAAGAATTCGAATAACAGACTCAAAGCACCAAAGATGGGAAATCCCACAACAAATAATCCCCCGCCAGACAACTTCACAACATCCACAACAATGTCAAACCCGCAACAAACACCTCGTGATCTCCAACGACCTTGTCTTCACTCTCCACAACACCACCCCATTCGGCTTCACCGTCACCCGCCAATCCTCCAAAGATGTCATTTTCGACTCCTCCCCCAACCCATCAAACCCCGACACCTTCTTGGTCTTCAAGGACCAATACATCCAGCTCTCCTCCTCCCTCCCCAAAGCCAGGTCCTCCCTTTTCGGCCTCGGCGAGCACACCAAGTCCTCCTTCAAGCTCACCCCCAACCAGACCTTGACACTCTGGACAGCTGACATTGGCAGCGCCAATGCTGACGTCAACCTCTACGGGTCCCACCCCTTCTACCTGGACGTCAGGTCAGCTTCTCCAGATGGCAAGGCCGACAGCGCCGGAACATCTCATGGAGTGTTGCTGCTGAATAGCAATGGCATGGACATAACATACGGCGGTGATAGAATTACTTATAAGGCCATTGGTGGAATTGTGGATTTGTATTTCTTTTCTGGGCCTACGCCGGAGTTGGTGGTGGAGCAGTATACAGAGCTCATTGGTCGCCCCACCCCTATGCCCTATTGGTCTTTTGGATTCCACCAGTGTCGATATGGTTACAAGAATGTTTCCGATCTTGAGGGTGTGGTTGCTGGCTACGCAAAAGCCGCTATCCCTCTTGAAGTTATGTGGACAGACATTGATTACATGGATGCATACAAGGATTTTACTCTTGATCCCATCAACTTCCCATTGGATAAGATGAAGAAATTTGTCAACACCCTTCACCAAAATGATCAGAAATATGTGCTCATCTTGGACCCTGGTATCAGTGTGAATAAGAGCTATGGAACCTACAACAGAGGGCTGAAAGCAGATATATTTATAAAACGTGACGGAATTCCTTACCTGGGAAGTGTTTGGCCTGGCCCTGTCTATTTCCCTGACTTTGCACATCCACAAAGTGAAAAATTTTGGGCTAATGAGATAAAAATATTTCAAGATGCTCTGCCTTTCGATGGTCTTTGGCTTGATATGAATGAGCTATCCAATTTCATCACGTCCCCTCCCACTCCATCTTCTACACTTGATGACCCTCCTTACAAGATTAACAATGCCGGGGTCCTGCGTCCCATCAATAACAATACTATTCCGGCATCAGCGCTACACTTTGGTAACATCACAGAATATGATGCTCATAACTTATATGGACTCTTGGAATCCAAAGCAACAAACAAGGCCTTAGTCAATGTGACTGGTAAAAGACCATTTATACTTTCTAGATCAACTTTTGTAAGCTCTGGTACGTACACAGCACACTGGACTGGAGACAATGCTGCAAAATGGAGTGATCTGGCATACACAATTCCAGCAATTCTGAATTTTGGCCTCTTCGGAGTTCCGATGGTTGGAGCTGATATATGTGGATTCTCTGGAAATACTACTGAAGAGCTCTGCAGGCGGTGGATTCAGCTAGGGGCCTTTTACCCTTTTGCAAGAGATCACTCAGAGAAGTTTACAATCCGTCAAGAGCTCTATCTTTGGGACTCAGTGGCTGCAACAGCAAGGAAGGTTCTTGGCCTGCGCTATCGGTTGCTCCCCATGTTTTACACATCAATGTATGAGGCACACAAAAAGGGGACCCCCATTGCAAGACCCCTTTTCTTCTCATTTCCTCAAGATATCAGAACTTATGAGATCAACACTCAGTTTCTGATTGGCAGAGGTGTAATGGTGTCACCTGTGTTGAAGCCAGGAGTAAGTTCAGTTGATGCATATTTTCCTGCAGGAAACTGGTTTGATCTCTTCAACTACTCAAACTCGGTAAGTGTAAAATCGGGAGAGCACGTTACACTGGATGCACCACCTGATCATATCAACGTACATGTCCGTGAAGGAAACATTTTGGCCTTACAAGGAGAGGCCTTGACAACAGAAGCAGCCCGAAAGACTGCATTCGAACTTTTGGTTGTTAGCAGCAGCAATGGACAGAGCACTGGAGAAGTTTTCTTGGATGATGGAGAGGAAGTGGAAATGGGAGGAAAGGGAGGCAAGTGGAGTTTAGTGAGATTTTACTGTGGAACAGCAAATGGAAGTGTATCTGTAAGATCAACGGTTGTAAATGGAGGATTTGCTTTAAGCCAAAAATGGATCATTGATAAAGTTACCATCATTGGGTTGGACAAGGTTGATGGATTGGAAAGGTATGCATTGAACATTACTAAGGGAGCAAATTTGAAGGGAGGACACTCAGATATCAGGGCCAGCTTTGACAGCAATAAGCGATTCGTGATGGTAGAGATTTCCAAATTATCCATTCTTATAGGAGCAGATTTTAACTTGGAGCTGAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

910

Amino Acids

101.21

Weight (kDa)

8.36

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 849, 1259
AatII GACGTC 2 cut(s) 702, 738
AccI GTMKAC 1 cut(s) 920
AccII CGCG 1 cut(s) 273
AciI CCGC 6 cut(s) 361, 400, 478, 831, 1041, 1834
AclWI GGATC 2 cut(s) 1103, 2531
AcsI RAATTY 7 cut(s) 165, 303, 1142, 1272, 1343, 1753, 2605
AcuI CTGAAG 1 cut(s) 1839
AcyI GRCGYC 2 cut(s) 699, 735
AdeI CACNNNGTG 2 cut(s) 415, 1337
AfaI GTAC 3 cut(s) 1678, 1682, 2256
AfeI AGCGCT 1 cut(s) 1536
AfiI CCNNNNNNNGG 7 cut(s) 294, 334, 591, 1283, 1391, 1781, 2547
AflIII ACRYGT 1 cut(s) 2257
AgsI TTSAA 8 cut(s) 550, 637, 1055, 1373, 2122, 2182, 2587, 2611
AhdI GACNNNNNGTC 1 cut(s) 739
AjiI CACGTC 1 cut(s) 1434
AjnI CCWGG 6 cut(s) 590, 729, 1195, 1281, 1297, 2125
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
Alw21I GWGCWC 7 cut(s) 621, 930, 1185, 1827, 1908, 2220, 2369
Alw26I GTCTC 1 cut(s) 1695
AlwI GGATC 2 cut(s) 1103, 2531
AlwNI CAGNNNCTG 3 cut(s) 149, 1928, 2086
Aor51HI AGCGCT 1 cut(s) 1536
ApeKI GCWGC 8 cut(s) 140, 684, 796, 1709, 1928, 2312, 2349, 2352
ApoI RAATTY 7 cut(s) 165, 303, 1142, 1272, 1343, 1753, 2605
AspLEI GCGC 5 cut(s) 275, 689, 773, 1537, 1959
AsuC2I CCSGG 1 cut(s) 1492
AsuHPI GGTGA 6 cut(s) 460, 466, 634, 845, 1151, 2103
AsuII TTCGAA 2 cut(s) 307, 2331
AvaII GGWCC 7 cut(s) 245, 553, 594, 714, 1192, 1495, 2013
BaeI ACNNNNGTAYC 2 cut(s) 1183, 1216
BanII GRGCYC 3 cut(s) 930, 1827, 1908
BauI CACGAG 1 cut(s) 413
BbsI GAAGAC 2 cut(s) 427, 538
Bbv12I GWGCWC 7 cut(s) 621, 930, 1185, 1827, 1908, 2220, 2369
BbvI GCAGC 8 cut(s) 127, 696, 783, 1696, 1915, 2324, 2361, 2364
BceAI ACGGC 1 cut(s) 844
BciT130I CCWGG 6 cut(s) 592, 731, 1197, 1283, 1299, 2127
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 2 cut(s) 1168, 2242
BcnI CCSGG 1 cut(s) 1492
BcoDI GTCTC 1 cut(s) 1695
BfaI CTAG 2 cut(s) 1653, 1847
BfmI CTRYAG 3 cut(s) 141, 1828, 2157
BfoI RGCGCY 3 cut(s) 690, 774, 1538
BfuI GTATCC 1 cut(s) 152
BisI GCNGC 9 cut(s) 141, 685, 797, 1041, 1710, 1929, 2313, 2350, 2353
BlsI GCNGC 9 cut(s) 142, 686, 798, 1042, 1711, 1930, 2314, 2351, 2354
Bme1390I CCNGG 7 cut(s) 592, 731, 1197, 1283, 1299, 1492, 2127
Bme18I GGWCC 7 cut(s) 245, 553, 594, 714, 1192, 1495, 2013
BmeRI GACNNNNNGTC 1 cut(s) 739
BmgBI CACGTC 1 cut(s) 1434
BmiI GGNNCC 9 cut(s) 247, 715, 716, 1194, 1225, 1496, 1852, 2014, 2015
BmrFI CCNGG 7 cut(s) 592, 731, 1197, 1283, 1299, 1492, 2127
BmsI GCATC 6 cut(s) 1075, 1366, 1538, 1555, 2134, 2221
BpiI GAAGAC 2 cut(s) 427, 538
BpmI CTGGAG 3 cut(s) 735, 1719, 2391
Bpu14I TTCGAA 2 cut(s) 307, 2331
BpuEI CTTGAG 2 cut(s) 1031, 2034
BpuMI CCSGG 1 cut(s) 1492
BsaAI YACGTR 1 cut(s) 1680
BsaHI GRCGYC 2 cut(s) 699, 735
BsaJI CCNNGG 5 cut(s) 123, 610, 1195, 1282, 1491
BsaXI ACNNNNNCTCC 7 cut(s) 1423, 1453, 2205, 2235, 2385, 2415, 2708
Bsc4I CCNNNNNNNGG 7 cut(s) 294, 334, 591, 1283, 1391, 1781, 2547
Bse1I ACTGG 7 cut(s) 976, 1633, 1697, 1702, 2171, 2232, 2374
Bse3DI GCAATG 3 cut(s) 814, 2019, 2362
BseBI CCWGG 6 cut(s) 592, 731, 1197, 1283, 1299, 2127
BseDI CCNNGG 5 cut(s) 123, 610, 1195, 1282, 1491
BseGI GGATG 7 cut(s) 252, 379, 561, 1090, 1327, 2236, 2392
BseLI CCNNNNNNNGG 7 cut(s) 294, 334, 591, 1283, 1391, 1781, 2547
BseMI GCAATG 3 cut(s) 814, 2019, 2362
BseMII CTCAG 4 cut(s) 1893, 1935, 2093, 2637
BseNI ACTGG 7 cut(s) 976, 1633, 1697, 1702, 2171, 2232, 2374
BseRI GAGGAG 3 cut(s) 499, 562, 565
BseXI GCAGC 8 cut(s) 127, 696, 783, 1696, 1915, 2324, 2361, 2364
BseYI CCCAGC 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 273
BsiHKAI GWGCWC 7 cut(s) 621, 930, 1185, 1827, 1908, 2220, 2369
BsiSI CCGG 4 cut(s) 774, 899, 1491, 1526
BslFI GGGAC 5 cut(s) 700, 1420, 1488, 1931, 2026
BslI CCNNNNNNNGG 7 cut(s) 294, 334, 591, 1283, 1391, 1781, 2547
BsmAI GTCTC 1 cut(s) 1695
BsmFI GGGAC 5 cut(s) 700, 1420, 1488, 1931, 2026
BsmI GAATGC 1 cut(s) 2327
Bsp119I TTCGAA 2 cut(s) 307, 2331
Bsp1286I GDGCHC 7 cut(s) 621, 930, 1185, 1827, 1908, 2220, 2369
BspACI CCGC 6 cut(s) 361, 400, 478, 831, 1041, 1834
BspCNI CTCAG 4 cut(s) 1892, 1934, 2092, 2636
BspFNI CGCG 1 cut(s) 273
BspLI GGNNCC 9 cut(s) 247, 715, 716, 1194, 1225, 1496, 1852, 2014, 2015
BspMAI CTGCAG 3 cut(s) 145, 1832, 2161
BspPI GGATC 2 cut(s) 1103, 2531
BspQI GCTCTTC 1 cut(s) 1815
BspT104I TTCGAA 2 cut(s) 307, 2331
BsrDI GCAATG 3 cut(s) 814, 2019, 2362
BsrI ACTGG 7 cut(s) 976, 1633, 1697, 1702, 2171, 2232, 2374
BssECI CCNNGG 5 cut(s) 123, 610, 1195, 1282, 1491
BssNAI GTATAC 1 cut(s) 921
BssNI GRCGYC 2 cut(s) 699, 735
BssSI CACGAG 1 cut(s) 413
BssT1I CCWWGG 1 cut(s) 123
Bst1107I GTATAC 1 cut(s) 921
Bst2BI CACGAG 1 cut(s) 413
Bst2UI CCWGG 6 cut(s) 592, 731, 1197, 1283, 1299, 2127
Bst4CI ACNGT 4 cut(s) 98, 472, 2450, 2488
Bst6I CTCTTC 4 cut(s) 62, 1769, 1815, 2183
BstACI GRCGYC 2 cut(s) 699, 735
BstBAI YACGTR 1 cut(s) 1680
BstBI TTCGAA 2 cut(s) 307, 2331
BstC8I GCNNGC 6 cut(s) 277, 617, 1027, 1832, 1955, 2638
BstDEI CTNAG 7 cut(s) 299, 1615, 1879, 1921, 2079, 2595, 2623
BstF5I GGATG 7 cut(s) 252, 379, 561, 1090, 1327, 2236, 2392
BstFNI CGCG 1 cut(s) 273
BstH2I RGCGCY 3 cut(s) 690, 774, 1538
BstHHI GCGC 5 cut(s) 275, 689, 773, 1537, 1959
BstMAI GTCTC 1 cut(s) 1695
BstMWI GCNNNNNNNGC 8 cut(s) 137, 146, 770, 1031, 1040, 1831, 1934, 2646
BstNI CCWGG 6 cut(s) 592, 731, 1197, 1283, 1299, 2127
BstNSI RCATGY 1 cut(s) 2261
BstSCI CCNGG 7 cut(s) 590, 729, 1195, 1281, 1297, 1490, 2125
BstSFI CTRYAG 3 cut(s) 141, 1828, 2157
BstSNI TACGTA 1 cut(s) 1680
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 8 cut(s) 127, 696, 783, 1696, 1915, 2324, 2361, 2364
BstV2I GAAGAC 2 cut(s) 427, 538
BstZ17I GTATAC 1 cut(s) 921
BsuI GTATCC 1 cut(s) 152
BtrI CACGTC 1 cut(s) 1434
BtsCI GGATG 7 cut(s) 252, 379, 561, 1090, 1327, 2236, 2392
BtsIMutI CAGTG 7 cut(s) 94, 983, 1210, 1690, 1929, 2225, 2367
Cac8I GCNNGC 6 cut(s) 277, 617, 1027, 1832, 1955, 2638
CaiI CAGNNNCTG 3 cut(s) 149, 1928, 2086
CfoI GCGC 5 cut(s) 275, 689, 773, 1537, 1959
CseI GACGC 1 cut(s) 1490
Csp6I GTAC 3 cut(s) 1677, 1681, 2255
CviAII CATG 5 cut(s) 785, 814, 1081, 1975, 2258
CviQI GTAC 3 cut(s) 1677, 1681, 2255
DdeI CTNAG 7 cut(s) 299, 1615, 1879, 1921, 2079, 2595, 2623
DraIII CACNNNGTG 2 cut(s) 415, 1337
DriI GACNNNNNGTC 1 cut(s) 739
Eam1104I CTCTTC 4 cut(s) 62, 1769, 1815, 2183
Eam1105I GACNNNNNGTC 1 cut(s) 739
EarI CTCTTC 4 cut(s) 62, 1769, 1815, 2183
Ecl136II GAGCTC 3 cut(s) 928, 1825, 1906
Eco105I TACGTA 1 cut(s) 1680
Eco130I CCWWGG 1 cut(s) 123
Eco147I AGGCCT 2 cut(s) 1613, 2297
Eco24I GRGCYC 3 cut(s) 930, 1827, 1908
Eco32I GATATC 2 cut(s) 2056, 2629
Eco47I GGWCC 7 cut(s) 245, 553, 594, 714, 1192, 1495, 2013
Eco47III AGCGCT 1 cut(s) 1536
Eco53kI GAGCTC 3 cut(s) 928, 1825, 1906
Eco57I CTGAAG 1 cut(s) 1839
EcoICRI GAGCTC 3 cut(s) 928, 1825, 1906
EcoO109I RGGNCCY 5 cut(s) 594, 714, 1495, 1851, 2013
EcoRI GAATTC 2 cut(s) 303, 1272
EcoRII CCWGG 6 cut(s) 590, 729, 1195, 1281, 1297, 2125
EcoRV GATATC 2 cut(s) 2056, 2629
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 3 cut(s) 1090, 2149, 2584
EcoT38I GRGCYC 3 cut(s) 930, 1827, 1908
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 5 cut(s) 788, 817, 1084, 1978, 2261
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 5 cut(s) 700, 1420, 1488, 1931, 2026
FatI CATG 5 cut(s) 784, 813, 1080, 1974, 2257
FauI CCCGC 3 cut(s) 368, 407, 485
FbaI TGATCA 2 cut(s) 1168, 2242
FblI GTMKAC 1 cut(s) 920
Fnu4HI GCNGC 9 cut(s) 141, 685, 797, 1041, 1710, 1929, 2313, 2350, 2353
FokI GGATG 7 cut(s) 239, 366, 548, 1097, 1314, 2243, 2399
FriOI GRGCYC 3 cut(s) 930, 1827, 1908
Fsp4HI GCNGC 9 cut(s) 141, 685, 797, 1041, 1710, 1929, 2313, 2350, 2353
FspBI CTAG 2 cut(s) 1653, 1847
GlaI GCGC 5 cut(s) 274, 688, 772, 1536, 1958
GluI GCNGC 9 cut(s) 141, 685, 797, 1041, 1710, 1929, 2313, 2350, 2353
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 3 cut(s) 735, 1719, 2391
HaeII RGCGCY 3 cut(s) 690, 774, 1538
HapII CCGG 4 cut(s) 774, 899, 1491, 1526
HgaI GACGC 1 cut(s) 1490
HhaI GCGC 5 cut(s) 275, 689, 773, 1537, 1959
Hin1I GRCGYC 2 cut(s) 699, 735
Hin1II CATG 5 cut(s) 788, 817, 1084, 1978, 2261
Hin6I GCGC 5 cut(s) 273, 687, 771, 1535, 1957
HinP1I GCGC 5 cut(s) 273, 687, 771, 1535, 1957
HincII GTYRAC 3 cut(s) 178, 703, 1150
HindII GTYRAC 3 cut(s) 178, 703, 1150
HpaII CCGG 4 cut(s) 774, 899, 1491, 1526
HphI GGTGA 6 cut(s) 460, 466, 634, 845, 1151, 2103
Hpy166II GTNNAC 7 cut(s) 178, 703, 921, 1065, 1150, 1683, 1890
Hpy188I TCNGA 9 cut(s) 1006, 1173, 1752, 1769, 1776, 1882, 2060, 2088, 2626
Hpy8I GTNNAC 7 cut(s) 178, 703, 921, 1065, 1150, 1683, 1890
HpyAV CCTTC 8 cut(s) 278, 547, 643, 733, 1166, 1936, 2261, 2605
HpyCH4III ACNGT 4 cut(s) 98, 472, 2450, 2488
HpyCH4IV ACGT 7 cut(s) 699, 735, 1264, 1433, 1679, 2220, 2253
HpyF10VI GCNNNNNNNGC 8 cut(s) 137, 146, 770, 1031, 1040, 1831, 1934, 2646
HpyF3I CTNAG 7 cut(s) 299, 1615, 1879, 1921, 2079, 2595, 2623
HpySE526I ACGT 7 cut(s) 699, 735, 1264, 1433, 1679, 2220, 2253
Hsp92I GRCGYC 2 cut(s) 699, 735
Hsp92II CATG 5 cut(s) 788, 817, 1084, 1978, 2261
HspAI GCGC 5 cut(s) 273, 687, 771, 1535, 1957
KflI GGGWCCC 2 cut(s) 714, 2013
Ksp22I TGATCA 2 cut(s) 1168, 2242
LguI GCTCTTC 1 cut(s) 1815
LmnI GCTCC 7 cut(s) 128, 913, 1784, 1974, 2600, 2699, 2716
Lsp1109I GCAGC 8 cut(s) 127, 696, 783, 1696, 1915, 2324, 2361, 2364
LweI GCATC 6 cut(s) 1075, 1366, 1538, 1555, 2134, 2221
MaeI CTAG 2 cut(s) 1653, 1847
MaeII ACGT 7 cut(s) 699, 735, 1264, 1433, 1679, 2220, 2253
MaeIII GTNAC 8 cut(s) 472, 989, 1265, 1547, 1624, 2109, 2221, 2536
MhlI GDGCHC 7 cut(s) 621, 930, 1185, 1827, 1908, 2220, 2369
MlyI GAGTC 4 cut(s) 311, 500, 1578, 1913
MmeI TCCRAC 3 cut(s) 447, 1762, 2532
Mph1103I ATGCAT 3 cut(s) 1090, 2149, 2584
MseI TTAA 3 cut(s) 1482, 2510, 2709
MslI CAYNNNNRTG 5 cut(s) 388, 947, 1164, 1988, 2262
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 4 cut(s) 774, 899, 1491, 1526
MspR9I CCNGG 7 cut(s) 592, 731, 1197, 1283, 1299, 1492, 2127
Mva1269I GAATGC 1 cut(s) 2327
MvaI CCWGG 6 cut(s) 592, 731, 1197, 1283, 1299, 2127
MvnI CGCG 1 cut(s) 273
MwoI GCNNNNNNNGC 8 cut(s) 137, 146, 770, 1031, 1040, 1831, 1934, 2646
NciI CCSGG 1 cut(s) 1492
NlaIII CATG 5 cut(s) 788, 817, 1084, 1978, 2261
NlaIV GGNNCC 9 cut(s) 247, 715, 716, 1194, 1225, 1496, 1852, 2014, 2015
NmeAIII GCCGAG 1 cut(s) 591
NmuCI GTSAC 4 cut(s) 472, 1265, 1624, 2109
NsiI ATGCAT 3 cut(s) 1090, 2149, 2584
NspI RCATGY 1 cut(s) 2261
NspV TTCGAA 2 cut(s) 307, 2331
PceI AGGCCT 2 cut(s) 1613, 2297
PciI ACATGT 1 cut(s) 2257
PciSI GCTCTTC 1 cut(s) 1815
PcsI WCGNNNNNNNCGW 2 cut(s) 468, 612
PctI GAATGC 1 cut(s) 2327
PfeI GAWTC 6 cut(s) 185, 969, 1592, 1800, 1840, 2658
PflFI GACNNNGTC 1 cut(s) 431
PkrI GCNGC 9 cut(s) 142, 686, 798, 1042, 1711, 1930, 2314, 2351, 2354
PleI GAGTC 4 cut(s) 311, 500, 1578, 1913
PpsI GAGTC 4 cut(s) 311, 500, 1578, 1913
Ppu21I YACGTR 1 cut(s) 1680
PpuMI RGGWCCY 4 cut(s) 594, 714, 1495, 2013
PscI ACATGT 1 cut(s) 2257
PsiI TTATAA 2 cut(s) 849, 1259
Psp124BI GAGCTC 3 cut(s) 930, 1827, 1908
Psp5II RGGWCCY 4 cut(s) 594, 714, 1495, 2013
Psp6I CCWGG 6 cut(s) 590, 729, 1195, 1281, 1297, 2125
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 6 cut(s) 590, 729, 1195, 1281, 1297, 2125
PspN4I GGNNCC 9 cut(s) 247, 715, 716, 1194, 1225, 1496, 1852, 2014, 2015
PspPPI RGGWCCY 4 cut(s) 594, 714, 1495, 2013
PstI CTGCAG 3 cut(s) 145, 1832, 2161
PstNI CAGNNNCTG 3 cut(s) 149, 1928, 2086
PsyI GACNNNGTC 1 cut(s) 431
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 3 cut(s) 1678, 1682, 2256
RsaNI GTAC 3 cut(s) 1677, 1681, 2255
RseI CAYNNNNRTG 5 cut(s) 388, 947, 1164, 1988, 2262
SacI GAGCTC 3 cut(s) 930, 1827, 1908
SapI GCTCTTC 1 cut(s) 1815
SaqAI TTAA 3 cut(s) 1482, 2510, 2709
SatI GCNGC 9 cut(s) 141, 685, 797, 1041, 1710, 1929, 2313, 2350, 2353
SbfI CCTGCAGG 1 cut(s) 2161
SchI GAGTC 4 cut(s) 311, 500, 1578, 1913
ScrFI CCNGG 7 cut(s) 592, 731, 1197, 1283, 1299, 1492, 2127
SdaI CCTGCAGG 1 cut(s) 2161
SduI GDGCHC 7 cut(s) 621, 930, 1185, 1827, 1908, 2220, 2369
SfaNI GCATC 6 cut(s) 1075, 1366, 1538, 1555, 2134, 2221
SfcI CTRYAG 3 cut(s) 141, 1828, 2157
SfuI TTCGAA 2 cut(s) 307, 2331
SinI GGWCC 7 cut(s) 245, 553, 594, 714, 1192, 1495, 2013
SmiMI CAYNNNNRTG 5 cut(s) 388, 947, 1164, 1988, 2262
SmlI CTYRAG 2 cut(s) 1010, 2049
SmoI CTYRAG 2 cut(s) 1010, 2049
SnaBI TACGTA 1 cut(s) 1680
Sse8387I CCTGCAGG 1 cut(s) 2161
SseBI AGGCCT 2 cut(s) 1613, 2297
SsiI CCGC 6 cut(s) 361, 400, 478, 831, 1041, 1834
SspI AATATT 1 cut(s) 1368
SspMI CTAG 2 cut(s) 1653, 1847
SstI GAGCTC 3 cut(s) 930, 1827, 1908
StuI AGGCCT 2 cut(s) 1613, 2297
StyD4I CCNGG 7 cut(s) 590, 729, 1195, 1281, 1297, 1490, 2125
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 4 cut(s) 98, 472, 2450, 2488
TaiI ACGT 7 cut(s) 702, 738, 1267, 1436, 1682, 2223, 2256
TaqI TCGA 5 cut(s) 307, 504, 982, 1389, 2331
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 6 cut(s) 185, 969, 1592, 1800, 1840, 2658
Tru1I TTAA 3 cut(s) 1482, 2510, 2709
Tru9I TTAA 3 cut(s) 1482, 2510, 2709
TscAI CASTG 7 cut(s) 101, 983, 1210, 1697, 1929, 2232, 2374
TseFI GTSAC 4 cut(s) 472, 1265, 1624, 2109
TseI GCWGC 8 cut(s) 140, 684, 796, 1709, 1928, 2312, 2349, 2352
Tsp45I GTSAC 4 cut(s) 472, 1265, 1624, 2109
TspDTI ATGAA 6 cut(s) 17, 99, 297, 1151, 1417, 1424
TspGWI ACGGA 4 cut(s) 258, 1284, 1886, 2252
TspRI CASTG 7 cut(s) 101, 983, 1210, 1697, 1929, 2232, 2374
Tth111I GACNNNGTC 1 cut(s) 431
VpaK11BI GGWCC 7 cut(s) 245, 553, 594, 714, 1192, 1495, 2013
XapI RAATTY 7 cut(s) 165, 303, 1142, 1272, 1343, 1753, 2605
XbaI TCTAGA 1 cut(s) 1652
XceI RCATGY 1 cut(s) 2261
XcmI CCANNNNNNNNNTGG 2 cut(s) 983, 2548
XmiI GTMKAC 1 cut(s) 920
XspI CTAG 2 cut(s) 1653, 1847
ZraI GACGTC 2 cut(s) 700, 736
Zsp2I ATGCAT 3 cut(s) 1090, 2149, 2584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.