Belongs to the glycosyl hydrolase 31 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 789 |
| AatII | GACGTC | 1 cut(s) | 438 |
| AccBSI | CCGCTC | 1 cut(s) | 423 |
| AccI | GTMKAC | 1 cut(s) | 1248 |
| AccII | CGCG | 1 cut(s) | 686 |
| AciI | CCGC | 9 cut(s) | 313, 385, 418, 421, 684, 771, 1821, 2097, 2177 |
| AclWI | GGATC | 6 cut(s) | 12, 301, 1043, 1127, 1140, 2471 |
| AcsI | RAATTY | 5 cut(s) | 114, 255, 1082, 1212, 1693 |
| AcuI | CTGAAG | 1 cut(s) | 1779 |
| AcyI | GRCGYC | 2 cut(s) | 435, 836 |
| AfaI | GTAC | 4 cut(s) | 818, 1276, 1622, 2159 |
| AfeI | AGCGCT | 1 cut(s) | 1476 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 714, 1129, 1223, 1331, 1721, 2487 |
| AgeI | ACCGGT | 1 cut(s) | 1902 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 679 |
| AjnI | CCWGG | 9 cut(s) | 143, 669, 1135, 1221, 1237, 1681, 1877, 2065, 2322 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 81, 113, 557, 589 |
| AleI | CACNNNNGTG | 1 cut(s) | 2609 |
| AluBI | AGCT | 7 cut(s) | 231, 1611, 1727, 1786, 1846, 2275, 2580 |
| AluI | AGCT | 7 cut(s) | 231, 1611, 1727, 1786, 1846, 2275, 2580 |
| Alw21I | GWGCWC | 3 cut(s) | 561, 1848, 2277 |
| Alw26I | GTCTC | 1 cut(s) | 693 |
| AlwI | GGATC | 6 cut(s) | 12, 301, 1043, 1127, 1140, 2471 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2026 |
| Aor51HI | AGCGCT | 1 cut(s) | 1476 |
| AoxI | GGCC | 7 cut(s) | 169, 547, 830, 1235, 1551, 1791, 2235 |
| ApeKI | GCWGC | 7 cut(s) | 92, 277, 733, 736, 1477, 1894, 2252 |
| ApoI | RAATTY | 5 cut(s) | 114, 255, 1082, 1212, 1693 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 425, 457 |
| AsiGI | ACCGGT | 1 cut(s) | 1902 |
| AspLEI | GCGC | 5 cut(s) | 1206, 1477, 1899, 2176, 2527 |
| AspS9I | GGNCC | 6 cut(s) | 197, 493, 654, 681, 830, 1791 |
| AsuHPI | GGTGA | 4 cut(s) | 406, 1091, 1253, 2043 |
| AsuII | TTCGAA | 1 cut(s) | 259 |
| AvaII | GGWCC | 4 cut(s) | 197, 493, 654, 681 |
| BamHI | GGATCC | 1 cut(s) | 1132 |
| BanII | GRGCYC | 2 cut(s) | 1848, 2277 |
| BbsI | GAAGAC | 2 cut(s) | 367, 478 |
| Bbv12I | GWGCWC | 3 cut(s) | 561, 1848, 2277 |
| BbvI | GCAGC | 7 cut(s) | 104, 289, 720, 723, 1464, 1881, 2264 |
| BccI | CCATC | 5 cut(s) | 279, 689, 1396, 1711, 1770 |
| BciT130I | CCWGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| BciVI | GTATCC | 2 cut(s) | 101, 995 |
| BclI | TGATCA | 1 cut(s) | 2182 |
| BcoDI | GTCTC | 1 cut(s) | 693 |
| BfaI | CTAG | 4 cut(s) | 777, 1593, 1667, 1787 |
| BfoI | RGCGCY | 1 cut(s) | 1478 |
| BfuI | GTATCC | 2 cut(s) | 101, 995 |
| BisI | GCNGC | 9 cut(s) | 93, 278, 421, 734, 737, 1478, 1895, 2177, 2253 |
| BlsI | GCNGC | 9 cut(s) | 94, 279, 422, 735, 738, 1479, 1896, 2178, 2254 |
| Bme1390I | CCNGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| Bme18I | GGWCC | 4 cut(s) | 197, 493, 654, 681 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 679 |
| BmgT120I | GGNCC | 6 cut(s) | 197, 493, 654, 681, 830, 1791 |
| BmiI | GGNNCC | 5 cut(s) | 655, 656, 1134, 1792, 1954 |
| BmrFI | CCNGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| BmsI | GCATC | 8 cut(s) | 25, 363, 700, 1015, 1306, 1478, 2074, 2161 |
| BpiI | GAAGAC | 2 cut(s) | 367, 478 |
| BpmI | CTGGAG | 4 cut(s) | 127, 346, 1452, 1659 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2538 |
| Bpu14I | TTCGAA | 1 cut(s) | 259 |
| BpuEI | CTTGAG | 2 cut(s) | 675, 1974 |
| Bsa29I | ATCGAT | 1 cut(s) | 1014 |
| BsaAI | YACGTR | 1 cut(s) | 1274 |
| BsaBI | GATNNNNATC | 1 cut(s) | 9 |
| BsaHI | GRCGYC | 2 cut(s) | 435, 836 |
| BsaJI | CCNNGG | 3 cut(s) | 550, 1222, 1536 |
| BsaWI | WCCGGW | 2 cut(s) | 1613, 1902 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 125, 155, 341, 371, 1363, 1393 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 714, 1129, 1223, 1331, 1721, 2487 |
| Bse118I | RCCGGY | 2 cut(s) | 1770, 1902 |
| Bse1I | ACTGG | 7 cut(s) | 902, 916, 981, 1573, 1637, 1642, 2111 |
| Bse3DI | GCAATG | 2 cut(s) | 1213, 2532 |
| Bse8I | GATNNNNATC | 1 cut(s) | 9 |
| BseBI | CCWGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| BseCI | ATCGAT | 1 cut(s) | 1014 |
| BseDI | CCNNGG | 3 cut(s) | 550, 1222, 1536 |
| BseGI | GGATG | 8 cut(s) | 204, 501, 1030, 1080, 1267, 1874, 2176, 2332 |
| BseJI | GATNNNNATC | 1 cut(s) | 9 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 714, 1129, 1223, 1331, 1721, 2487 |
| BseMI | GCAATG | 2 cut(s) | 1213, 2532 |
| BseMII | CTCAG | 2 cut(s) | 831, 1875 |
| BseNI | ACTGG | 7 cut(s) | 902, 916, 981, 1573, 1637, 1642, 2111 |
| BseRI | GAGGAG | 6 cut(s) | 175, 349, 415, 502, 1368, 2367 |
| BseXI | GCAGC | 7 cut(s) | 104, 289, 720, 723, 1464, 1881, 2264 |
| BseYI | CCCAGC | 1 cut(s) | 827 |
| BsgI | GTGCAG | 2 cut(s) | 1463, 1635 |
| Bsh1236I | CGCG | 1 cut(s) | 686 |
| BshFI | GGCC | 7 cut(s) | 171, 549, 832, 1237, 1553, 1793, 2237 |
| BshTI | ACCGGT | 1 cut(s) | 1902 |
| BshVI | ATCGAT | 1 cut(s) | 1014 |
| BsiHKAI | GWGCWC | 3 cut(s) | 561, 1848, 2277 |
| BsiSI | CCGG | 4 cut(s) | 1466, 1614, 1771, 1903 |
| BslFI | GGGAC | 3 cut(s) | 640, 730, 1871 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 714, 1129, 1223, 1331, 1721, 2487 |
| BsmAI | GTCTC | 1 cut(s) | 693 |
| BsmBI | CGTCTC | 1 cut(s) | 693 |
| BsmFI | GGGAC | 3 cut(s) | 640, 730, 1871 |
| BsmI | GAATGC | 3 cut(s) | 616, 1686, 2267 |
| BsnI | GGCC | 7 cut(s) | 171, 549, 832, 1237, 1553, 1793, 2237 |
| Bsp119I | TTCGAA | 1 cut(s) | 259 |
| Bsp1286I | GDGCHC | 3 cut(s) | 561, 1848, 2277 |
| BspACI | CCGC | 9 cut(s) | 313, 385, 418, 421, 684, 771, 1821, 2097, 2177 |
| BspANI | GGCC | 7 cut(s) | 171, 549, 832, 1237, 1553, 1793, 2237 |
| BspCNI | CTCAG | 2 cut(s) | 832, 1874 |
| BspDI | ATCGAT | 1 cut(s) | 1014 |
| BspFNI | CGCG | 1 cut(s) | 686 |
| BspLI | GGNNCC | 5 cut(s) | 655, 656, 1134, 1792, 1954 |
| BspPI | GGATC | 6 cut(s) | 12, 301, 1043, 1127, 1140, 2471 |
| BspT104I | TTCGAA | 1 cut(s) | 259 |
| BsrBI | CCGCTC | 1 cut(s) | 423 |
| BsrDI | GCAATG | 2 cut(s) | 1213, 2532 |
| BsrFI | RCCGGY | 2 cut(s) | 1770, 1902 |
| BsrI | ACTGG | 7 cut(s) | 902, 916, 981, 1573, 1637, 1642, 2111 |
| BssAI | RCCGGY | 2 cut(s) | 1770, 1902 |
| BssECI | CCNNGG | 3 cut(s) | 550, 1222, 1536 |
| BssNI | GRCGYC | 2 cut(s) | 435, 836 |
| BssT1I | CCWWGG | 1 cut(s) | 1536 |
| Bst2UI | CCWGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| Bst4CI | ACNGT | 6 cut(s) | 412, 1334, 1462, 1581, 2157, 2608 |
| Bst6I | CTCTTC | 3 cut(s) | 520, 1101, 2123 |
| BstACI | GRCGYC | 2 cut(s) | 435, 836 |
| BstBAI | YACGTR | 1 cut(s) | 1274 |
| BstBI | TTCGAA | 1 cut(s) | 259 |
| BstC8I | GCNNGC | 3 cut(s) | 229, 557, 1772 |
| BstDEI | CTNAG | 4 cut(s) | 251, 840, 1861, 2538 |
| BstEII | GGTNACC | 1 cut(s) | 2476 |
| BstF5I | GGATG | 8 cut(s) | 204, 501, 1030, 1080, 1267, 1874, 2176, 2332 |
| BstFNI | CGCG | 1 cut(s) | 686 |
| BstH2I | RGCGCY | 1 cut(s) | 1478 |
| BstHHI | GCGC | 5 cut(s) | 1206, 1477, 1899, 2176, 2527 |
| BstMAI | GTCTC | 1 cut(s) | 693 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 92, 971, 1894, 2173, 2543 |
| BstNI | CCWGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| BstNSI | RCATGY | 1 cut(s) | 2201 |
| BstPI | GGTNACC | 1 cut(s) | 2476 |
| BstSCI | CCNGG | 9 cut(s) | 143, 669, 1135, 1221, 1237, 1681, 1877, 2065, 2322 |
| BstUI | CGCG | 1 cut(s) | 686 |
| BstV1I | GCAGC | 7 cut(s) | 104, 289, 720, 723, 1464, 1881, 2264 |
| BstV2I | GAAGAC | 2 cut(s) | 367, 478 |
| BstX2I | RGATCY | 1 cut(s) | 1132 |
| BstYI | RGATCY | 1 cut(s) | 1132 |
| Bsu15I | ATCGAT | 1 cut(s) | 1014 |
| BsuI | GTATCC | 2 cut(s) | 101, 995 |
| BsuRI | GGCC | 7 cut(s) | 171, 549, 832, 1237, 1553, 1793, 2237 |
| BsuTUI | ATCGAT | 1 cut(s) | 1014 |
| BtgZI | GCGATG | 1 cut(s) | 1789 |
| BtsCI | GGATG | 8 cut(s) | 204, 501, 1030, 1080, 1267, 1874, 2176, 2332 |
| BtsI | GCAGTG | 2 cut(s) | 349, 2673 |
| BtsIMutI | CAGTG | 6 cut(s) | 349, 408, 923, 1630, 1869, 2673 |
| Cac8I | GCNNGC | 3 cut(s) | 229, 557, 1772 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2026 |
| CfoI | GCGC | 5 cut(s) | 1206, 1477, 1899, 2176, 2527 |
| Cfr10I | RCCGGY | 2 cut(s) | 1770, 1902 |
| Cfr13I | GGNCC | 6 cut(s) | 197, 493, 654, 681, 830, 1791 |
| ClaI | ATCGAT | 1 cut(s) | 1014 |
| CseI | GACGC | 4 cut(s) | 616, 675, 844, 1430 |
| Csp6I | GTAC | 4 cut(s) | 817, 1275, 1621, 2158 |
| CspAI | ACCGGT | 1 cut(s) | 1902 |
| CviAII | CATG | 6 cut(s) | 304, 725, 754, 1021, 2198, 2507 |
| CviQI | GTAC | 4 cut(s) | 817, 1275, 1621, 2158 |
| DdeI | CTNAG | 4 cut(s) | 251, 840, 1861, 2538 |
| DriI | GACNNNNNGTC | 1 cut(s) | 679 |
| Eam1104I | CTCTTC | 3 cut(s) | 520, 1101, 2123 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 679 |
| EarI | CTCTTC | 3 cut(s) | 520, 1101, 2123 |
| EciI | GGCGGA | 1 cut(s) | 1810 |
| Ecl136II | GAGCTC | 2 cut(s) | 1846, 2275 |
| Eco130I | CCWWGG | 1 cut(s) | 1536 |
| Eco147I | AGGCCT | 2 cut(s) | 1553, 2237 |
| Eco24I | GRGCYC | 2 cut(s) | 1848, 2277 |
| Eco32I | GATATC | 1 cut(s) | 1359 |
| Eco47I | GGWCC | 4 cut(s) | 197, 493, 654, 681 |
| Eco47III | AGCGCT | 1 cut(s) | 1476 |
| Eco53kI | GAGCTC | 2 cut(s) | 1846, 2275 |
| Eco57I | CTGAAG | 1 cut(s) | 1779 |
| Eco91I | GGTNACC | 1 cut(s) | 2476 |
| EcoICRI | GAGCTC | 2 cut(s) | 1846, 2275 |
| EcoO109I | RGGNCCY | 2 cut(s) | 654, 1791 |
| EcoO65I | GGTNACC | 1 cut(s) | 2476 |
| EcoRI | GAATTC | 3 cut(s) | 114, 255, 1212 |
| EcoRII | CCWGG | 9 cut(s) | 143, 669, 1135, 1221, 1237, 1681, 1877, 2065, 2322 |
| EcoRV | GATATC | 1 cut(s) | 1359 |
| EcoT14I | CCWWGG | 1 cut(s) | 1536 |
| EcoT22I | ATGCAT | 1 cut(s) | 2089 |
| EcoT38I | GRGCYC | 2 cut(s) | 1848, 2277 |
| ErhI | CCWWGG | 1 cut(s) | 1536 |
| Esp3I | CGTCTC | 1 cut(s) | 693 |
| FaeI | CATG | 6 cut(s) | 307, 728, 757, 1024, 2201, 2510 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 557, 589, 2465, 2497 |
| FaqI | GGGAC | 3 cut(s) | 640, 730, 1871 |
| FatI | CATG | 6 cut(s) | 303, 724, 753, 1020, 2197, 2506 |
| FauI | CCCGC | 3 cut(s) | 320, 425, 2104 |
| FbaI | TGATCA | 1 cut(s) | 2182 |
| FblI | GTMKAC | 1 cut(s) | 1248 |
| Fnu4HI | GCNGC | 9 cut(s) | 93, 278, 421, 734, 737, 1478, 1895, 2177, 2253 |
| FokI | GGATG | 8 cut(s) | 191, 488, 1037, 1087, 1254, 1861, 2183, 2339 |
| FriOI | GRGCYC | 2 cut(s) | 1848, 2277 |
| Fsp4HI | GCNGC | 9 cut(s) | 93, 278, 421, 734, 737, 1478, 1895, 2177, 2253 |
| FspBI | CTAG | 4 cut(s) | 777, 1593, 1667, 1787 |
| GlaI | GCGC | 5 cut(s) | 1205, 1476, 1898, 2175, 2526 |
| GluI | GCNGC | 9 cut(s) | 93, 278, 421, 734, 737, 1478, 1895, 2177, 2253 |
| GsaI | CCCAGC | 1 cut(s) | 831 |
| GsuI | CTGGAG | 4 cut(s) | 127, 346, 1452, 1659 |
| HaeII | RGCGCY | 1 cut(s) | 1478 |
| HaeIII | GGCC | 7 cut(s) | 171, 549, 832, 1237, 1553, 1793, 2237 |
| HapII | CCGG | 4 cut(s) | 1466, 1614, 1771, 1903 |
| HgaI | GACGC | 4 cut(s) | 616, 675, 844, 1430 |
| HhaI | GCGC | 5 cut(s) | 1206, 1477, 1899, 2176, 2527 |
| Hin1I | GRCGYC | 2 cut(s) | 435, 836 |
| Hin1II | CATG | 6 cut(s) | 307, 728, 757, 1024, 2201, 2510 |
| Hin6I | GCGC | 5 cut(s) | 1204, 1475, 1897, 2174, 2525 |
| HinP1I | GCGC | 5 cut(s) | 1204, 1475, 1897, 2174, 2525 |
| HincII | GTYRAC | 4 cut(s) | 127, 156, 1057, 1090 |
| HindII | GTYRAC | 4 cut(s) | 127, 156, 1057, 1090 |
| HindIII | AAGCTT | 1 cut(s) | 2578 |
| HpaI | GTTAAC | 1 cut(s) | 1090 |
| HpaII | CCGG | 4 cut(s) | 1466, 1614, 1771, 1903 |
| HphI | GGTGA | 4 cut(s) | 406, 1091, 1253, 2043 |
| Hpy99I | CGWCG | 2 cut(s) | 437, 838 |
| HpyAV | CCTTC | 8 cut(s) | 487, 583, 891, 1876, 2201, 2491, 2510, 2548 |
| HpyCH4III | ACNGT | 6 cut(s) | 412, 1334, 1462, 1581, 2157, 2608 |
| HpyCH4IV | ACGT | 4 cut(s) | 435, 675, 1273, 2193 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 92, 971, 1894, 2173, 2543 |
| HpyF3I | CTNAG | 4 cut(s) | 251, 840, 1861, 2538 |
| HpySE526I | ACGT | 4 cut(s) | 435, 675, 1273, 2193 |
| Hsp92I | GRCGYC | 2 cut(s) | 435, 836 |
| Hsp92II | CATG | 6 cut(s) | 307, 728, 757, 1024, 2201, 2510 |
| HspAI | GCGC | 5 cut(s) | 1204, 1475, 1897, 2174, 2525 |
| KflI | GGGWCCC | 1 cut(s) | 654 |
| KroI | GCCGGC | 1 cut(s) | 1770 |
| KroNI | GCCGGC | 1 cut(s) | 1772 |
| Ksp22I | TGATCA | 1 cut(s) | 2182 |
| KspAI | GTTAAC | 1 cut(s) | 1090 |
| LmnI | GCTCC | 6 cut(s) | 428, 1616, 1724, 1914, 2543, 2639 |
| Lsp1109I | GCAGC | 7 cut(s) | 104, 289, 720, 723, 1464, 1881, 2264 |
| LweI | GCATC | 8 cut(s) | 25, 363, 700, 1015, 1306, 1478, 2074, 2161 |
| MaeI | CTAG | 4 cut(s) | 777, 1593, 1667, 1787 |
| MaeII | ACGT | 4 cut(s) | 435, 675, 1273, 2193 |
| MaeIII | GTNAC | 7 cut(s) | 412, 929, 1145, 1487, 1564, 2476, 2602 |
| MbiI | CCGCTC | 1 cut(s) | 423 |
| MfeI | CAATTG | 2 cut(s) | 75, 1959 |
| MflI | RGATCY | 1 cut(s) | 1132 |
| MhlI | GDGCHC | 3 cut(s) | 561, 1848, 2277 |
| MlyI | GAGTC | 6 cut(s) | 263, 595, 1443, 1518, 1853, 2079 |
| MmeI | TCCRAC | 6 cut(s) | 453, 529, 1702, 2472, 2634, 2643 |
| Mph1103I | ATGCAT | 1 cut(s) | 2089 |
| MroNI | GCCGGC | 1 cut(s) | 1770 |
| MseI | TTAA | 5 cut(s) | 1089, 1422, 1556, 2450, 2649 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1104, 1928, 2567, 2609 |
| MspA1I | CMGCKG | 2 cut(s) | 771, 2099 |
| MspI | CCGG | 4 cut(s) | 1466, 1614, 1771, 1903 |
| MspR9I | CCNGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| MunI | CAATTG | 2 cut(s) | 75, 1959 |
| Mva1269I | GAATGC | 3 cut(s) | 616, 1686, 2267 |
| MvaI | CCWGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| MvnI | CGCG | 1 cut(s) | 686 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 92, 971, 1894, 2173, 2543 |
| NaeI | GCCGGC | 1 cut(s) | 1772 |
| NgoMIV | GCCGGC | 1 cut(s) | 1770 |
| NlaIII | CATG | 6 cut(s) | 307, 728, 757, 1024, 2201, 2510 |
| NlaIV | GGNNCC | 5 cut(s) | 655, 656, 1134, 1792, 1954 |
| NmeAIII | GCCGAG | 1 cut(s) | 531 |
| NmuCI | GTSAC | 2 cut(s) | 412, 2602 |
| NsiI | ATGCAT | 1 cut(s) | 2089 |
| NspI | RCATGY | 1 cut(s) | 2201 |
| NspV | TTCGAA | 1 cut(s) | 259 |
| OliI | CACNNNNGTG | 1 cut(s) | 2609 |
| PceI | AGGCCT | 2 cut(s) | 1553, 2237 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 441, 552, 2451 |
| PctI | GAATGC | 3 cut(s) | 616, 1686, 2267 |
| PdiI | GCCGGC | 1 cut(s) | 1772 |
| PfeI | GAWTC | 7 cut(s) | 137, 909, 1427, 1740, 1780, 2442, 2629 |
| PflFI | GACNNNGTC | 1 cut(s) | 371 |
| PfoI | TCCNGGA | 3 cut(s) | 143, 1877, 2322 |
| PinAI | ACCGGT | 1 cut(s) | 1902 |
| PkrI | GCNGC | 9 cut(s) | 94, 279, 422, 735, 738, 1479, 1896, 2178, 2254 |
| PleI | GAGTC | 6 cut(s) | 263, 595, 1442, 1518, 1853, 2078 |
| PpsI | GAGTC | 6 cut(s) | 263, 595, 1442, 1518, 1853, 2078 |
| Ppu21I | YACGTR | 1 cut(s) | 1274 |
| PpuMI | RGGWCCY | 1 cut(s) | 654 |
| PsiI | TTATAA | 1 cut(s) | 789 |
| Psp124BI | GAGCTC | 2 cut(s) | 1848, 2277 |
| Psp5II | RGGWCCY | 1 cut(s) | 654 |
| Psp6I | CCWGG | 9 cut(s) | 143, 669, 1135, 1221, 1237, 1681, 1877, 2065, 2322 |
| PspEI | GGTNACC | 1 cut(s) | 2476 |
| PspFI | CCCAGC | 1 cut(s) | 827 |
| PspGI | CCWGG | 9 cut(s) | 143, 669, 1135, 1221, 1237, 1681, 1877, 2065, 2322 |
| PspN4I | GGNNCC | 5 cut(s) | 655, 656, 1134, 1792, 1954 |
| PspPI | GGNCC | 6 cut(s) | 197, 493, 654, 681, 830, 1791 |
| PspPPI | RGGWCCY | 1 cut(s) | 654 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2026 |
| PsuI | RGATCY | 1 cut(s) | 1132 |
| PsyI | GACNNNGTC | 1 cut(s) | 371 |
| RsaI | GTAC | 4 cut(s) | 818, 1276, 1622, 2159 |
| RsaNI | GTAC | 4 cut(s) | 817, 1275, 1621, 2158 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1104, 1928, 2567, 2609 |
| SacI | GAGCTC | 2 cut(s) | 1848, 2277 |
| SaqAI | TTAA | 5 cut(s) | 1089, 1422, 1556, 2450, 2649 |
| SatI | GCNGC | 9 cut(s) | 93, 278, 421, 734, 737, 1478, 1895, 2177, 2253 |
| Sau96I | GGNCC | 6 cut(s) | 197, 493, 654, 681, 830, 1791 |
| SchI | GAGTC | 6 cut(s) | 263, 595, 1443, 1518, 1853, 2079 |
| ScrFI | CCNGG | 9 cut(s) | 145, 671, 1137, 1223, 1239, 1683, 1879, 2067, 2324 |
| SduI | GDGCHC | 3 cut(s) | 561, 1848, 2277 |
| SfaNI | GCATC | 8 cut(s) | 25, 363, 700, 1015, 1306, 1478, 2074, 2161 |
| SfuI | TTCGAA | 1 cut(s) | 259 |
| SinI | GGWCC | 4 cut(s) | 197, 493, 654, 681 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1104, 1928, 2567, 2609 |
| SmlI | CTYRAG | 2 cut(s) | 690, 1989 |
| SmoI | CTYRAG | 2 cut(s) | 690, 1989 |
| SseBI | AGGCCT | 2 cut(s) | 1553, 2237 |
| SsiI | CCGC | 9 cut(s) | 313, 385, 418, 421, 684, 771, 1821, 2097, 2177 |
| SspI | AATATT | 3 cut(s) | 1286, 1308, 2215 |
| SspMI | CTAG | 4 cut(s) | 777, 1593, 1667, 1787 |
| SstI | GAGCTC | 2 cut(s) | 1848, 2277 |
| StuI | AGGCCT | 2 cut(s) | 1553, 2237 |
| StyD4I | CCNGG | 9 cut(s) | 143, 669, 1135, 1221, 1237, 1681, 1877, 2065, 2322 |
| StyI | CCWWGG | 1 cut(s) | 1536 |
| TaaI | ACNGT | 6 cut(s) | 412, 1334, 1462, 1581, 2157, 2608 |
| TaiI | ACGT | 4 cut(s) | 438, 678, 1276, 2196 |
| TaqI | TCGA | 8 cut(s) | 259, 444, 922, 1014, 1047, 2129, 2271, 2627 |
| TaqII | GACCGA | 1 cut(s) | 722 |
| TatI | WGTACW | 3 cut(s) | 816, 1620, 2157 |
| TauI | GCSGC | 2 cut(s) | 423, 2179 |
| TfiI | GAWTC | 7 cut(s) | 137, 909, 1427, 1740, 1780, 2442, 2629 |
| Tru1I | TTAA | 5 cut(s) | 1089, 1422, 1556, 2450, 2649 |
| Tru9I | TTAA | 5 cut(s) | 1089, 1422, 1556, 2450, 2649 |
| TscAI | CASTG | 6 cut(s) | 356, 415, 923, 1637, 1869, 2680 |
| TseFI | GTSAC | 2 cut(s) | 412, 2602 |
| TseI | GCWGC | 7 cut(s) | 92, 277, 733, 736, 1477, 1894, 2252 |
| Tsp45I | GTSAC | 2 cut(s) | 412, 2602 |
| TspDTI | ATGAA | 7 cut(s) | 249, 1091, 1176, 1186, 1357, 1364, 2473 |
| TspGWI | ACGGA | 6 cut(s) | 34, 210, 1022, 1826, 2313, 2346 |
| TspRI | CASTG | 6 cut(s) | 356, 415, 923, 1637, 1869, 2680 |
| Tth111I | GACNNNGTC | 1 cut(s) | 371 |
| VpaK11BI | GGWCC | 4 cut(s) | 197, 493, 654, 681 |
| XapI | RAATTY | 5 cut(s) | 114, 255, 1082, 1212, 1693 |
| XbaI | TCTAGA | 1 cut(s) | 1592 |
| XceI | RCATGY | 1 cut(s) | 2201 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 478, 923 |
| XmiI | GTMKAC | 1 cut(s) | 1248 |
| XspI | CTAG | 4 cut(s) | 777, 1593, 1667, 1787 |
| ZraI | GACGTC | 1 cut(s) | 436 |
| Zsp2I | ATGCAT | 1 cut(s) | 2089 |