Rh5DG136700

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000882
Physical Location & Seq
Forward (+)
13712919 .. 13717890
4972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG136700.1

Sequence Viewer

Length: 2523 bp
ATGGCTAATCTTGGCCTGATCAATAGCTCCTCAATCTATGGACCTGACATCCCAAATCTAAAGCTTTATGCCAGCTATGAAACCAAAGAAAGACTAAGAATTCGAATAACAGACTCGAGTAACCAAAGATGGGAAATCCCACAAGACCTTCTCCCTCGACAGACTCCACAAACATCCCACCATTCGATCCCCGAAAACGATCTCATTTTCACCCTCCACACCACCACCCCGTTTGGCTTCACGGTCAACCGCCGCTTCTCCAATGATGTCGTATTCGACGCCACCCCAAACCCCTCCAACCCTTCCTCCATCCTCGTCTTCAAGGACCAGTACATCCAGCTTTCCTCCTCTCTCCCGGAAACCCGATCTTCCCTGTACGGCCTCGGTGAGCACACCAAAGCCTCCTTCAAGCTCCAATCCAATCAGACTCTAACACTCTGGACTGCCGACATTTTCAGCTCCATCCCCGACCTCAATCTCTATGGGTCCCACCCATTTTACATGGACGTGAGGTCACCGTCCGGTGATGAAAAAGTTACTGCAGGAACCACTCATGGAGTTTTGCTATTGAATAGTAATGGCATGGATGTAAATTACGACGGCGACAGGATTACATATAAAGTGATTGGCGGAGTTGTTGACTTGTATTTTTTTTCTGGGCCGACGCCGGAATTGGTGATGGAGCAATACACGGAGTTCATTGGCCGACCTGCCCCGATGCCCTACTGGTCTTTTGGATTCCACCAATGTAGATATAGTTACAAGGATGTTGCTGACCTTGAGGAGGTGGTTGCTGGTTATGCAAATGCTAGCATCCCTCTTGAAGGTATTTGGACAGACATTGACTACATGGATGCTTACAAGGATTTCACTCTTGATCCCATCAACTTTCCATTAAGTAAGATGCAGAACTTTACAAACACCCTTCACCAAAATGGCCAGAAATATGTGCCGATCTTGGACCCTGGTATTAGTATAAATGAGAGCTATGGAAGTTACATCAGAGGGAAGGAAGCAGATATATACATAAAACGTGATGGTATTCCTTACCAGGGACATGTATGGCCTGGTGATGTTAACTATCCTGATTTTGTACATCCACAAAGTGAACAATTTTGGGCTAATGAGATCAAACTATTTCAAGACCAACTAGCTTTTGATGGTCTTTGGCTTGATATGAATGAGCCATGCAATTTGATAACATCTGCTCCCACTCCATATTCTACACTAGATGATCCACCTTACAAAATCAATGATTCCGGAGTCCAGCGTCCGACCATTAATAAGACTGTTCCGGCATCAGCTCTACACTTTGGTAATATAACAGAGTATAATGTTCATAACCTATATGGGTTCTTGGAATCTAAAGCAACACACCAGGGGTTAATCAATGTCACTGGTAAGAGACCATTTATACTCACTAGATCAACCTTTGTAAGCTCTGGTAAGTACACAGCACACTGGACTGGAGACAATGCAGCAAGATGGAGTGACTTGGCATACACAATTCCAGGCATTTTGAATTTTGGACTCTTCGGAGTTCCGATGGTTGGAGCTGATATATGTGGATTTACTTTGAATACTACTGAAGAACTCTGCAGGCGATGGATCCAGCTAGGGGCCTTTTATCCTTTTGCAAGAGATCATTCAGATATATTTACAATCCGCCAAGAGCTCTATGTTTGGGATTCAGTTGCTGCATCAGCAAGGAAGGCGCTAGGCCTCCGTTACCGGTTGCTCCCCTTGTTCTACACATCAATGTATCAGGCACACAAAAAAGGGACCCCCATTGCAAGGCCTCTTTTCTTCTCGTTTCCTGAAGATACCAATACATACAACATCAATTCACAGTTCTTGATTGGTAAAGGTGTGATGGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTTATGCATATGTTCCTGCTGGAAACTGGTTTGATCTCTTCAACTACTCCAGATCAGTGAGTGTAGACTCCGGAGAGTATGTTATGTTGGACGCACCGCCTGATCATATCAATGTACATGTCCGCGAAGGAAACATTTTGGCCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCTCGGAAGACTGCATTCGAACTTTTGGTGGTCATTAGTAGTAGCGGAGAGAGCACTGGAGAAGTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTAGTGAAATTCTATTGTGGAGCAGCAAACGGAAGTGTACTTTTGAGCTCAAAAGTTGAAAATGGAGGATTTGCTTTGAGCCAAAAATGGATCATTGATAAAGTTACCTTCATTGGGTTGGAAAAAGTTGATGGATTAGAAGGGTTTGCAGTCAACTTTATTAAGGGAACAAATTTGAAAGGAATGTGGGATGTCAATGCCAACTTTCACGGTGATAAGCGTTTTGTCATGGTAAAAATATCCAGTGTATCCATTCCTATAGGAAAAGAGTTTGAGTTGGAGCTGAGCCTGAAGCGTGACACCGGTGAAAACACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

840

Amino Acids

93.96

Weight (kDa)

5.24

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 32 - 192 1.6e-09 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 237 - 585 2.1e-118 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 594 - 683 2e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 718
AccI GTMKAC 1 cut(s) 1974
AccII CGCG 1 cut(s) 2034
AccIII TCCGGA 2 cut(s) 1259, 1979
AciI CCGC 7 cut(s) 250, 253, 630, 1666, 2006, 2032, 2127
AclWI GGATC 6 cut(s) 181, 872, 1229, 1603, 1616, 2300
AcoI YGGCCR 2 cut(s) 703, 937
AcsI RAATTY 5 cut(s) 99, 1522, 1903, 2210, 2374
AcuI CTGAAG 3 cut(s) 1608, 1839, 2513
AcyI GRCGYC 2 cut(s) 279, 665
AdeI CACNNNGTG 1 cut(s) 1106
AfaI GTAC 6 cut(s) 332, 377, 1095, 1451, 2025, 2241
AfiI CCNNNNNNNGG 8 cut(s) 130, 784, 824, 1052, 1550, 1618, 1794, 2316
AflIII ACRYGT 2 cut(s) 1057, 2026
AgeI ACCGGT 2 cut(s) 1731, 2504
AhdI GACNNNNNGTC 1 cut(s) 511
AjiI CACGTC 1 cut(s) 508
AjnI CCWGG 5 cut(s) 964, 1050, 1066, 1377, 1510
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
Alw21I GWGCWC 4 cut(s) 393, 1677, 2138, 2252
Alw26I GTCTC 2 cut(s) 1399, 1464
AlwI GGATC 6 cut(s) 181, 872, 1229, 1603, 1616, 2300
AlwNI CAGNNNCTG 1 cut(s) 1697
Ama87I CYCGRG 1 cut(s) 115
Aor13HI TCCGGA 2 cut(s) 1259, 1979
ApeKI GCWGC 5 cut(s) 1478, 1697, 1891, 2081, 2225
ApoI RAATTY 5 cut(s) 99, 1522, 1903, 2210, 2374
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AseI ATTAAT 1 cut(s) 1281
AsiGI ACCGGT 2 cut(s) 1731, 2504
AspLEI GCGC 1 cut(s) 1717
AspS9I GGNCC 7 cut(s) 41, 325, 486, 659, 961, 1620, 1782
AsuC2I CCSGG 1 cut(s) 356
AsuII TTCGAA 2 cut(s) 103, 2100
AsuNHI GCTAGC 1 cut(s) 809
AvaI CYCGRG 1 cut(s) 115
AvaII GGWCC 5 cut(s) 41, 325, 486, 961, 1782
BalI TGGCCA 1 cut(s) 939
BamHI GGATCC 1 cut(s) 1608
BanII GRGCYC 2 cut(s) 1677, 2252
BbsI GAAGAC 2 cut(s) 310, 2096
Bbv12I GWGCWC 4 cut(s) 393, 1677, 2138, 2252
BbvI GCAGC 5 cut(s) 1490, 1684, 1903, 2093, 2237
BceAI ACGGC 2 cut(s) 394, 616
BciT130I CCWGG 5 cut(s) 966, 1052, 1068, 1379, 1512
BciVI GTATCC 1 cut(s) 2461
BclI TGATCA 2 cut(s) 18, 2011
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 2 cut(s) 1399, 1464
BfaI CTAG 6 cut(s) 810, 1151, 1229, 1422, 1616, 1718
BfmI CTRYAG 3 cut(s) 540, 1597, 2460
BfoI RGCGCY 1 cut(s) 1718
BfuAI ACCTGC 1 cut(s) 718
BfuI GTATCC 1 cut(s) 2461
BisI GCNGC 6 cut(s) 253, 1479, 1698, 1892, 2082, 2226
BlpI GCTNAGC 1 cut(s) 2486
BlsI GCNGC 6 cut(s) 254, 1480, 1699, 1893, 2083, 2227
Bme1390I CCNGG 6 cut(s) 356, 966, 1052, 1068, 1379, 1512
Bme18I GGWCC 5 cut(s) 41, 325, 486, 961, 1782
BmeRI GACNNNNNGTC 1 cut(s) 511
BmeT110I CYCGRG 1 cut(s) 115
BmgBI CACGTC 1 cut(s) 508
BmgT120I GGNCC 7 cut(s) 41, 325, 486, 659, 961, 1620, 1782
BmiI GGNNCC 8 cut(s) 487, 488, 547, 963, 1610, 1621, 1783, 1784
BmrFI CCNGG 6 cut(s) 356, 966, 1052, 1068, 1379, 1512
BmsI GCATC 6 cut(s) 708, 822, 844, 894, 1307, 1709
BmtI GCTAGC 1 cut(s) 813
BpiI GAAGAC 2 cut(s) 310, 2096
BpmI CTGGAG 3 cut(s) 1488, 1942, 2160
Bpu1102I GCTNAGC 1 cut(s) 2486
Bpu14I TTCGAA 2 cut(s) 103, 2100
BpuEI CTTGAG 1 cut(s) 800
BpuMI CCSGG 1 cut(s) 356
BsaHI GRCGYC 2 cut(s) 279, 665
BsaI GGTCTC 1 cut(s) 1399
BsaJI CCNNGG 4 cut(s) 382, 964, 1051, 1378
BsaWI WCCGGW 5 cut(s) 521, 1259, 1731, 1979, 2504
BsaXI ACNNNNNCTCC 8 cut(s) 290, 320, 1192, 1222, 1974, 2004, 2154, 2184
Bsc4I CCNNNNNNNGG 8 cut(s) 130, 784, 824, 1052, 1550, 1618, 1794, 2316
Bse118I RCCGGY 2 cut(s) 1731, 2504
Bse1I ACTGG 8 cut(s) 328, 731, 1402, 1466, 1471, 1940, 2143, 2445
Bse3DI GCAATG 1 cut(s) 1788
BseAI TCCGGA 2 cut(s) 1259, 1979
BseBI CCWGG 5 cut(s) 966, 1052, 1068, 1379, 1512
BseDI CCNNGG 4 cut(s) 382, 964, 1051, 1378
BseLI CCNNNNNNNGG 8 cut(s) 130, 784, 824, 1052, 1550, 1618, 1794, 2316
BseMI GCAATG 1 cut(s) 1788
BseMII CTCAG 1 cut(s) 2477
BseNI ACTGG 8 cut(s) 328, 731, 1402, 1466, 1471, 1940, 2143, 2445
BseRI GAGGAG 4 cut(s) 19, 337, 797, 2196
BseXI GCAGC 5 cut(s) 1490, 1684, 1903, 2093, 2237
Bsh1236I CGCG 1 cut(s) 2034
BshTI ACCGGT 2 cut(s) 1731, 2504
BsiHKAI GWGCWC 4 cut(s) 393, 1677, 2138, 2252
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 8 cut(s) 356, 522, 668, 1260, 1295, 1732, 1980, 2505
BslFI GGGAC 3 cut(s) 472, 1068, 1795
BslI CCNNNNNNNGG 8 cut(s) 130, 784, 824, 1052, 1550, 1618, 1794, 2316
BsmAI GTCTC 2 cut(s) 1399, 1464
BsmFI GGGAC 3 cut(s) 472, 1068, 1795
BsmI GAATGC 1 cut(s) 2096
Bso31I GGTCTC 1 cut(s) 1399
BsoBI CYCGRG 1 cut(s) 115
Bsp119I TTCGAA 2 cut(s) 103, 2100
Bsp1286I GDGCHC 4 cut(s) 393, 1677, 2138, 2252
Bsp13I TCCGGA 2 cut(s) 1259, 1979
Bsp1407I TGTACA 2 cut(s) 1093, 2023
Bsp1720I GCTNAGC 1 cut(s) 2486
BspACI CCGC 7 cut(s) 250, 253, 630, 1666, 2006, 2032, 2127
BspCNI CTCAG 1 cut(s) 2478
BspEI TCCGGA 2 cut(s) 1259, 1979
BspFNI CGCG 1 cut(s) 2034
BspLI GGNNCC 8 cut(s) 487, 488, 547, 963, 1610, 1621, 1783, 1784
BspMAI CTGCAG 2 cut(s) 544, 1601
BspMI ACCTGC 1 cut(s) 718
BspOI GCTAGC 1 cut(s) 813
BspPI GGATC 6 cut(s) 181, 872, 1229, 1603, 1616, 2300
BspT104I TTCGAA 2 cut(s) 103, 2100
BspTNI GGTCTC 1 cut(s) 1399
BsrDI GCAATG 1 cut(s) 1788
BsrFI RCCGGY 2 cut(s) 1731, 2504
BsrGI TGTACA 2 cut(s) 1093, 2023
BsrI ACTGG 8 cut(s) 328, 731, 1402, 1466, 1471, 1940, 2143, 2445
BssAI RCCGGY 2 cut(s) 1731, 2504
BssECI CCNNGG 4 cut(s) 382, 964, 1051, 1378
BssNI GRCGYC 2 cut(s) 279, 665
Bst2UI CCWGG 5 cut(s) 966, 1052, 1068, 1379, 1512
Bst4CI ACNGT 5 cut(s) 244, 519, 1291, 1851, 2414
Bst6I CTCTTC 2 cut(s) 1538, 1952
BstACI GRCGYC 2 cut(s) 279, 665
BstAUI TGTACA 2 cut(s) 1093, 2023
BstBI TTCGAA 2 cut(s) 103, 2100
BstC8I GCNNGC 3 cut(s) 73, 811, 1601
BstDEI CTNAG 2 cut(s) 95, 2486
BstEII GGTNACC 1 cut(s) 513
BstENI CCTNNNNNAGG 2 cut(s) 782, 822
BstFNI CGCG 1 cut(s) 2034
BstH2I RGCGCY 1 cut(s) 1718
BstHHI GCGC 1 cut(s) 1717
BstMAI GTCTC 2 cut(s) 1399, 1464
BstMWI GCNNNNNNNGC 4 cut(s) 800, 1703, 1712, 2133
BstNI CCWGG 5 cut(s) 966, 1052, 1068, 1379, 1512
BstNSI RCATGY 2 cut(s) 1061, 2030
BstPI GGTNACC 1 cut(s) 513
BstSCI CCNGG 6 cut(s) 354, 964, 1050, 1066, 1377, 1510
BstSFI CTRYAG 3 cut(s) 540, 1597, 2460
BstUI CGCG 1 cut(s) 2034
BstV1I GCAGC 5 cut(s) 1490, 1684, 1903, 2093, 2237
BstV2I GAAGAC 2 cut(s) 310, 2096
BstX2I RGATCY 1 cut(s) 1608
BstYI RGATCY 1 cut(s) 1608
BsuI GTATCC 1 cut(s) 2461
BtgZI GCGATG 1 cut(s) 1618
BtrI CACGTC 1 cut(s) 508
BtsIMutI CAGTG 5 cut(s) 1395, 1459, 1971, 2136, 2452
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 3 cut(s) 73, 811, 1601
CaiI CAGNNNCTG 1 cut(s) 1697
CfoI GCGC 1 cut(s) 1717
Cfr10I RCCGGY 2 cut(s) 1731, 2504
Cfr13I GGNCC 7 cut(s) 41, 325, 486, 659, 961, 1620, 1782
CseI GACGC 4 cut(s) 287, 673, 1259, 2009
Csp6I GTAC 6 cut(s) 331, 376, 1094, 1450, 2024, 2240
CspAI ACCGGT 2 cut(s) 1731, 2504
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 8 cut(s) 502, 554, 583, 850, 1058, 1188, 2027, 2431
CviQI GTAC 6 cut(s) 331, 376, 1094, 1450, 2024, 2240
DdeI CTNAG 2 cut(s) 95, 2486
DraIII CACNNNGTG 1 cut(s) 1106
DriI GACNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 703, 937
Eam1104I CTCTTC 2 cut(s) 1538, 1952
Eam1105I GACNNNNNGTC 1 cut(s) 511
EarI CTCTTC 2 cut(s) 1538, 1952
EciI GGCGGA 2 cut(s) 645, 1655
Ecl136II GAGCTC 2 cut(s) 1675, 2250
Eco147I AGGCCT 3 cut(s) 1722, 1798, 2066
Eco24I GRGCYC 2 cut(s) 1677, 2252
Eco31I GGTCTC 1 cut(s) 1399
Eco47I GGWCC 5 cut(s) 41, 325, 486, 961, 1782
Eco53kI GAGCTC 2 cut(s) 1675, 2250
Eco57I CTGAAG 3 cut(s) 1608, 1839, 2513
Eco88I CYCGRG 1 cut(s) 115
Eco91I GGTNACC 1 cut(s) 513
EcoICRI GAGCTC 2 cut(s) 1675, 2250
EcoNI CCTNNNNNAGG 2 cut(s) 782, 822
EcoO109I RGGNCCY 3 cut(s) 486, 1620, 1782
EcoO65I GGTNACC 1 cut(s) 513
EcoRI GAATTC 1 cut(s) 99
EcoRII CCWGG 5 cut(s) 964, 1050, 1066, 1377, 1510
EcoT22I ATGCAT 1 cut(s) 1918
EcoT38I GRGCYC 2 cut(s) 1677, 2252
FaeI CATG 8 cut(s) 505, 557, 586, 853, 1061, 1191, 2030, 2434
FaqI GGGAC 3 cut(s) 472, 1068, 1795
FatI CATG 8 cut(s) 501, 553, 582, 849, 1057, 1187, 2026, 2430
FauNDI CATATG 1 cut(s) 1918
FbaI TGATCA 2 cut(s) 18, 2011
FblI GTMKAC 1 cut(s) 1974
Fnu4HI GCNGC 6 cut(s) 253, 1479, 1698, 1892, 2082, 2226
FriOI GRGCYC 2 cut(s) 1677, 2252
Fsp4HI GCNGC 6 cut(s) 253, 1479, 1698, 1892, 2082, 2226
FspBI CTAG 6 cut(s) 810, 1151, 1229, 1422, 1616, 1718
GlaI GCGC 1 cut(s) 1716
GluI GCNGC 6 cut(s) 253, 1479, 1698, 1892, 2082, 2226
GsuI CTGGAG 3 cut(s) 1488, 1942, 2160
HaeII RGCGCY 1 cut(s) 1718
HapII CCGG 8 cut(s) 356, 522, 668, 1260, 1295, 1732, 1980, 2505
HgaI GACGC 4 cut(s) 287, 673, 1259, 2009
HhaI GCGC 1 cut(s) 1717
Hin1I GRCGYC 2 cut(s) 279, 665
Hin1II CATG 8 cut(s) 505, 557, 586, 853, 1061, 1191, 2030, 2434
Hin6I GCGC 1 cut(s) 1715
HinP1I GCGC 1 cut(s) 1715
HincII GTYRAC 4 cut(s) 247, 640, 1078, 2356
HindII GTYRAC 4 cut(s) 247, 640, 1078, 2356
HindIII AAGCTT 1 cut(s) 62
HpaI GTTAAC 1 cut(s) 1078
HpaII CCGG 8 cut(s) 356, 522, 668, 1260, 1295, 1732, 1980, 2505
Hpy166II GTNNAC 8 cut(s) 247, 640, 1078, 1109, 1452, 1975, 2240, 2356
Hpy188I TCNGA 7 cut(s) 426, 1004, 1275, 1538, 1545, 1651, 2088
Hpy8I GTNNAC 8 cut(s) 247, 640, 1078, 1109, 1452, 1975, 2240, 2356
Hpy99I CGWCG 3 cut(s) 281, 602, 667
HpyCH4III ACNGT 5 cut(s) 244, 519, 1291, 1851, 2414
HpyCH4IV ACGT 2 cut(s) 507, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 800, 1703, 1712, 2133
HpyF3I CTNAG 2 cut(s) 95, 2486
HpySE526I ACGT 2 cut(s) 507, 1033
Hsp92I GRCGYC 2 cut(s) 279, 665
Hsp92II CATG 8 cut(s) 505, 557, 586, 853, 1061, 1191, 2030, 2434
HspAI GCGC 1 cut(s) 1715
KflI GGGWCCC 2 cut(s) 486, 1782
Kpn2I TCCGGA 2 cut(s) 1259, 1979
Ksp22I TGATCA 2 cut(s) 18, 2011
KspAI GTTAAC 1 cut(s) 1078
Lsp1109I GCAGC 5 cut(s) 1490, 1684, 1903, 2093, 2237
LweI GCATC 6 cut(s) 708, 822, 844, 894, 1307, 1709
MaeI CTAG 6 cut(s) 810, 1151, 1229, 1422, 1616, 1718
MaeII ACGT 2 cut(s) 507, 1033
MboII GAAGA 8 cut(s) 310, 360, 1525, 1601, 1798, 1832, 1939, 2101
MflI RGATCY 1 cut(s) 1608
MhlI GDGCHC 4 cut(s) 393, 1677, 2138, 2252
MlsI TGGCCA 1 cut(s) 939
MluNI TGGCCA 1 cut(s) 939
MlyI GAGTC 6 cut(s) 107, 157, 421, 1272, 1524, 1970
MmeI TCCRAC 6 cut(s) 321, 1298, 1531, 1977, 2301, 2460
Mox20I TGGCCA 1 cut(s) 939
Mph1103I ATGCAT 1 cut(s) 1918
MroI TCCGGA 2 cut(s) 1259, 1979
MscI TGGCCA 1 cut(s) 939
MseI TTAA 6 cut(s) 896, 1077, 1281, 1385, 2364, 2521
MslI CAYNNNNRTG 4 cut(s) 506, 933, 1757, 2019
Msp20I TGGCCA 1 cut(s) 939
MspI CCGG 8 cut(s) 356, 522, 668, 1260, 1295, 1732, 1980, 2505
MspR9I CCNGG 6 cut(s) 356, 966, 1052, 1068, 1379, 1512
Mva1269I GAATGC 1 cut(s) 2096
MvaI CCWGG 5 cut(s) 966, 1052, 1068, 1379, 1512
MvnI CGCG 1 cut(s) 2034
MwoI GCNNNNNNNGC 4 cut(s) 800, 1703, 1712, 2133
NciI CCSGG 1 cut(s) 356
NdeI CATATG 1 cut(s) 1918
NheI GCTAGC 1 cut(s) 809
NlaIII CATG 8 cut(s) 505, 557, 586, 853, 1061, 1191, 2030, 2434
NlaIV GGNNCC 8 cut(s) 487, 488, 547, 963, 1610, 1621, 1783, 1784
NmuCI GTSAC 5 cut(s) 513, 1393, 1490, 1878, 2498
NsiI ATGCAT 1 cut(s) 1918
NspI RCATGY 2 cut(s) 1061, 2030
NspV TTCGAA 2 cut(s) 103, 2100
PaeR7I CTCGAG 1 cut(s) 115
PceI AGGCCT 3 cut(s) 1722, 1798, 2066
PciI ACATGT 2 cut(s) 1057, 2026
PctI GAATGC 1 cut(s) 2096
PfeI GAWTC 4 cut(s) 738, 1256, 1361, 1688
PfoI TCCNGGA 1 cut(s) 354
PinAI ACCGGT 2 cut(s) 1731, 2504
PkrI GCNGC 6 cut(s) 254, 1480, 1699, 1893, 2083, 2227
PleI GAGTC 6 cut(s) 107, 157, 421, 1271, 1524, 1970
PpsI GAGTC 6 cut(s) 107, 157, 421, 1271, 1524, 1970
PpuMI RGGWCCY 2 cut(s) 486, 1782
PscI ACATGT 2 cut(s) 1057, 2026
PshBI ATTAAT 1 cut(s) 1281
Psp124BI GAGCTC 2 cut(s) 1677, 2252
Psp5II RGGWCCY 2 cut(s) 486, 1782
Psp6I CCWGG 5 cut(s) 964, 1050, 1066, 1377, 1510
PspEI GGTNACC 1 cut(s) 513
PspGI CCWGG 5 cut(s) 964, 1050, 1066, 1377, 1510
PspN4I GGNNCC 8 cut(s) 487, 488, 547, 963, 1610, 1621, 1783, 1784
PspPI GGNCC 7 cut(s) 41, 325, 486, 659, 961, 1620, 1782
PspPPI RGGWCCY 2 cut(s) 486, 1782
PspXI VCTCGAGB 1 cut(s) 115
PstI CTGCAG 2 cut(s) 544, 1601
PstNI CAGNNNCTG 1 cut(s) 1697
PsuI RGATCY 1 cut(s) 1608
RsaI GTAC 6 cut(s) 332, 377, 1095, 1451, 2025, 2241
RsaNI GTAC 6 cut(s) 331, 376, 1094, 1450, 2024, 2240
RseI CAYNNNNRTG 4 cut(s) 506, 933, 1757, 2019
SacI GAGCTC 2 cut(s) 1677, 2252
SaqAI TTAA 6 cut(s) 896, 1077, 1281, 1385, 2364, 2521
SatI GCNGC 6 cut(s) 253, 1479, 1698, 1892, 2082, 2226
Sau96I GGNCC 7 cut(s) 41, 325, 486, 659, 961, 1620, 1782
SchI GAGTC 6 cut(s) 107, 157, 421, 1272, 1524, 1970
ScrFI CCNGG 6 cut(s) 356, 966, 1052, 1068, 1379, 1512
SduI GDGCHC 4 cut(s) 393, 1677, 2138, 2252
SfaNI GCATC 6 cut(s) 708, 822, 844, 894, 1307, 1709
SfcI CTRYAG 3 cut(s) 540, 1597, 2460
Sfr274I CTCGAG 1 cut(s) 115
SfuI TTCGAA 2 cut(s) 103, 2100
SgrAI CRCCGGYG 1 cut(s) 2504
SinI GGWCC 5 cut(s) 41, 325, 486, 961, 1782
SlaI CTCGAG 1 cut(s) 115
SmiMI CAYNNNNRTG 4 cut(s) 506, 933, 1757, 2019
SmlI CTYRAG 2 cut(s) 115, 779
SmoI CTYRAG 2 cut(s) 115, 779
SseBI AGGCCT 3 cut(s) 1722, 1798, 2066
SsiI CCGC 7 cut(s) 250, 253, 630, 1666, 2006, 2032, 2127
SspMI CTAG 6 cut(s) 810, 1151, 1229, 1422, 1616, 1718
SstI GAGCTC 2 cut(s) 1677, 2252
StuI AGGCCT 3 cut(s) 1722, 1798, 2066
StyD4I CCNGG 6 cut(s) 354, 964, 1050, 1066, 1377, 1510
TaaI ACNGT 5 cut(s) 244, 519, 1291, 1851, 2414
TaiI ACGT 2 cut(s) 510, 1036
TaqI TCGA 7 cut(s) 103, 116, 157, 185, 276, 2100, 2172
TatI WGTACW 5 cut(s) 330, 1093, 1449, 2023, 2239
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 4 cut(s) 738, 1256, 1361, 1688
Tru1I TTAA 6 cut(s) 896, 1077, 1281, 1385, 2364, 2521
Tru9I TTAA 6 cut(s) 896, 1077, 1281, 1385, 2364, 2521
TscAI CASTG 5 cut(s) 1402, 1466, 1971, 2143, 2452
TseFI GTSAC 5 cut(s) 513, 1393, 1490, 1878, 2498
TseI GCWGC 5 cut(s) 1478, 1697, 1891, 2081, 2225
Tsp45I GTSAC 5 cut(s) 513, 1393, 1490, 1878, 2498
TspDTI ATGAA 6 cut(s) 93, 543, 688, 1193, 1328, 2302
TspGWI ACGGA 3 cut(s) 707, 1715, 2247
TspRI CASTG 5 cut(s) 1402, 1466, 1971, 2143, 2452
VpaK11BI GGWCC 5 cut(s) 41, 325, 486, 961, 1782
VspI ATTAAT 1 cut(s) 1281
XagI CCTNNNNNAGG 2 cut(s) 782, 822
XapI RAATTY 5 cut(s) 99, 1522, 1903, 2210, 2374
XceI RCATGY 2 cut(s) 1061, 2030
XhoI CTCGAG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 1974
XspI CTAG 6 cut(s) 810, 1151, 1229, 1422, 1616, 1718
Zsp2I ATGCAT 1 cut(s) 1918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.