Prupe.4G103500_v2.0.a1

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000882
Physical Location & Seq
Forward (+)
5293273 .. 5299176
5904 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G103500.3

Sequence Viewer

Length: 2727 bp
ATGAAGATGAAAAGAAGCAGACCAAACAAACCAAAACCAATATATCATCTTCCACTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTCTGCTGTCTTTCACTGTGGTTTAGTTTCATCATCAAATACCAAGGGAACAAAAGAAGCTGCAGTTGCTGGGTTTGGATACAAAATTCAGTCAGTCAACTATGAATCTTCTGGGAACTCATTGACTGCTAATCTTGGCCTCATCAAGAAATCCTCCCTCTACGGACCTGACATCCCAAATCTCAACCTTCACGCCAGCTGTGAAACCAAAGATCGGCTAAGAATTCGAATAACAGACTCAAAGCACCAAAGATGGGAAATCCCACAACAAATAATTCCCCGCCAAACAACTTCACAGCATCCACAACAATGTCAAACCCACAACAAACACCTCGTGATCTCCAACGACCTTGTCTTCACTCTCCACAACACCACCCCATTCGGCTTCACGGTCACCCGCCAATCCTCCAACGACGTCATTTTCGACGCCTCCCCAAACCCATCAAACCCCGACACCTTCTTGGTCTTCAAGGACCAATACATCCAGCTCTCCTCCTCCCTCCCCGAAGCCAGGTCCTCCCTTTTTGGCCTCGGCGAGCACACTTCCTCCTTCAAGCTCACCCCCAACCAGACCTTGACACTCTGGAACGCTGACACTGCCAGCGCCAATGCTGACATCAACCTCTACGGGTCCCACCCCTTCTACCTGGACGTCAGGTCAGCTTCTCCAGATGGCAAGGCCAACGGCGCCGGAACATCTCATGGAGTGTTGCTGCTGAATAGCAATGGCATGGACATAACATACGGCGGTGATAGAATTACTTATAAGGCCATTGGTGGAATTGTGGATTTGTATTTCTTTTCTGGGCCAACACCGGAGTTGGTGGTGGAGCAGTATACAGAGCTCATTGGTCGCCCTACCCCTATGCCCTATTGGTCTTTTGGATTCCACCAGTGTCGATGGGGTTACAAGAATGTTTCCGATCTTGAGGGTGTGGTTGCTGGCTATGAAAAAGCCGCTATCCCTCTTGAAGTTATGTGGACAGACATTGATTACATGGATGCATACAAGGATTTTACTCTTGATCCCATCAACTTCCCATTGGATAAGATGAAGAAATTTGTCAACACCCTTCACCAAAATGATCAGAAATATGTGCTCATCTTGGACCCTGGTATCAGTGTGAATGAGAGCTATGGAACCTACAACAGAGGGCTGAAAGCAGATATATTTATAAAACGTGACGGAATTCCTTACCTGGGAAGTGTTTGGCCTGGCCCTGTCTATTTCCCTGACTTTGCACATCCACAAAGTGAAAAAATTTGGGCTAATGAGATAAAAATATTTCAAGATGCTCTGCCTTTCGATGGTCTTTGGCTTGATATGAATGAGCTATCCAATTTCATCACGTCGCCTGCCACTCCATCTTCTACACTTGATGACCCTCCTTACAAGATTAACAATGCCGGGGTCCTGCGTCCCATCAATAACAGTACTGTTCCGGCATCAGCGCTACACTTTGGTAACATCACAGAATATGATGCTCATAACTTATATGGACTCTTGGAAACCAAAGCAACAAACAAGGCCTTAGTCAATGTGACTGGTAAAAGACCATTTATACTTTCTAGATCAACTTTTGTAAGCTCTGGTACGTACACAGCACACTGGACTGGAGACAATGCTGCAAAATGGAGTGATCTGGCATACACAATTCCAGCAATTCTGAATTTTGGCCTCTTCGGAGTTCCGATGGTTGGAGCTGATATATGTGGATTCTCTGGAAATACTACTGAAGAGCTCTGCAGGCGGTGGATTCAGCTAGGGGCCTTTTACCCTTTTGCAAGAGATCACTCAGAGAAGTTTACAATCCGTCAAGAGCTCTATCTTTGGGACTCGGTGGCTGCAACTGCAAGGAAGGTGCTTGGCCTGCGCTATCGGTTGCTCCCCTTGTTTTACACATCAATGTATGAGGCACATAAAAAGGGCACCCCCATTGCAAGACCCCTTTTCTTCTCATTTCCTCAAGATATCAGAACTTATGAGATCAACACTCAGTTTCTGATTGGCAGAGGTGTAATGGTGTCACCTGTGTTGAAGCCAGGAGTAAGTTCAGTTGATGCATATTTTCCTGCAGGAAACTGGTTTAATCTCTTCAACTACTCAAACTCAGTAAGCGTAAAATCGGGAGAGCACGTTACACTGGAAGCACCACCTGATCATATCAACGTACATGTCTGCGAAGGAAACATTTTGGCCTTACAAGGAAAGGCCTTGACAACAGAAGCAGCCCGAAAGACTGCGTTCGAACTTTTGGTTGTTAGCAGCAGTGGACAGAGCACCGGAGAAGTTTTCTTGGATGATGGAGAGGAAGTGGAAATGGGAGGAGAGGGAGGCAAGTGGAGTTTAGTGAGATTTTACGGTGGAAAAAAAAATGGAAGTGTATCTGTAAGATCAACGGTTGTCAATGGAGGATTTGCTTTAAGCCAAAAATGGATCATTGATAAAGTTACCATCATTGGATTGGAAAAGGTTGATGGATTGGAAGGGTATGCATTGAACATTACTAAGGGAGCAAACTTGAAGCGGGGACACTCAGATATCAGGGCCAGCTTTGACAGCAATAAGCGATTCATCACAGTAGAGATTTCCAAATTATCCATTCTTATAGGAGCAGATTTTAACTTGGAGCTGAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

909

Amino Acids

100.97

Weight (kDa)

7.1

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 849, 1259
AatII GACGTC 2 cut(s) 501, 738
AccB1I GGYRCC 2 cut(s) 770, 2012
AccI GTMKAC 1 cut(s) 920
AciI CCGC 6 cut(s) 364, 481, 831, 1041, 1834, 2611
AclWI GGATC 2 cut(s) 1103, 2528
AcsI RAATTY 6 cut(s) 168, 306, 1142, 1272, 1344, 1753
AcuI CTGAAG 1 cut(s) 1839
AcyI GRCGYC 4 cut(s) 498, 510, 735, 771
AdeI CACNNNGTG 2 cut(s) 418, 1337
AfaI GTAC 4 cut(s) 1519, 1678, 1682, 2256
AfeI AGCGCT 1 cut(s) 1536
AfiI CCNNNNNNNGG 6 cut(s) 297, 337, 594, 1283, 1391, 1781
AflIII ACRYGT 1 cut(s) 2257
AgsI TTSAA 8 cut(s) 553, 637, 1055, 1373, 2122, 2182, 2584, 2608
AhdI GACNNNNNGTC 1 cut(s) 739
AjiI CACGTC 1 cut(s) 1434
AjnI CCWGG 6 cut(s) 593, 729, 1195, 1281, 1297, 2125
Alw21I GWGCWC 7 cut(s) 624, 930, 1185, 1827, 1908, 2220, 2366
Alw26I GTCTC 1 cut(s) 1695
AlwI GGATC 2 cut(s) 1103, 2528
AlwNI CAGNNNCTG 2 cut(s) 152, 2086
Aor51HI AGCGCT 1 cut(s) 1536
ApeKI GCWGC 6 cut(s) 143, 796, 1709, 1928, 2312, 2349
ApoI RAATTY 6 cut(s) 168, 306, 1142, 1272, 1344, 1753
ArsI GACNNNNNNTTYG 2 cut(s) 488, 520
AspLEI GCGC 4 cut(s) 689, 773, 1537, 1959
AsuC2I CCSGG 1 cut(s) 1492
AsuHPI GGTGA 5 cut(s) 469, 634, 845, 1151, 2103
AsuII TTCGAA 2 cut(s) 310, 2331
AvaII GGWCC 6 cut(s) 248, 556, 597, 714, 1192, 1495
BaeGI GKGCMC 1 cut(s) 2015
BaeI ACNNNNGTAYC 2 cut(s) 1183, 1216
BanI GGYRCC 2 cut(s) 770, 2012
BanII GRGCYC 3 cut(s) 930, 1827, 1908
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 2 cut(s) 430, 541
Bbv12I GWGCWC 7 cut(s) 624, 930, 1185, 1827, 1908, 2220, 2366
BbvI GCAGC 6 cut(s) 130, 783, 1696, 1915, 2324, 2361
BceAI ACGGC 2 cut(s) 784, 844
BciT130I CCWGG 6 cut(s) 595, 731, 1197, 1283, 1299, 2127
BciVI GTATCC 1 cut(s) 155
BclI TGATCA 2 cut(s) 1168, 2242
BcnI CCSGG 1 cut(s) 1492
BcoDI GTCTC 1 cut(s) 1695
BfaI CTAG 2 cut(s) 1653, 1847
BfmI CTRYAG 3 cut(s) 144, 1828, 2157
BfoI RGCGCY 3 cut(s) 690, 774, 1538
BfuI GTATCC 1 cut(s) 155
BisI GCNGC 7 cut(s) 144, 797, 1041, 1710, 1929, 2313, 2350
BlsI GCNGC 7 cut(s) 145, 798, 1042, 1711, 1930, 2314, 2351
BmcAI AGTACT 1 cut(s) 1519
Bme1390I CCNGG 7 cut(s) 595, 731, 1197, 1283, 1299, 1492, 2127
Bme18I GGWCC 6 cut(s) 248, 556, 597, 714, 1192, 1495
BmeRI GACNNNNNGTC 1 cut(s) 739
BmgBI CACGTC 1 cut(s) 1434
BmiI GGNNCC 8 cut(s) 715, 716, 772, 1194, 1225, 1496, 1852, 2014
BmrFI CCNGG 7 cut(s) 595, 731, 1197, 1283, 1299, 1492, 2127
BmsI GCATC 6 cut(s) 391, 1075, 1366, 1538, 1555, 2134
BpiI GAAGAC 2 cut(s) 430, 541
BpmI CTGGAG 2 cut(s) 735, 1719
Bpu14I TTCGAA 2 cut(s) 310, 2331
BpuEI CTTGAG 2 cut(s) 1031, 2034
BpuMI CCSGG 1 cut(s) 1492
BsaAI YACGTR 1 cut(s) 1680
BsaHI GRCGYC 4 cut(s) 498, 510, 735, 771
BsaJI CCNNGG 5 cut(s) 126, 613, 1195, 1282, 1491
BsaWI WCCGGW 2 cut(s) 898, 2366
BsaXI ACNNNNNCTCC 7 cut(s) 614, 644, 2205, 2235, 2382, 2412, 2705
Bsc4I CCNNNNNNNGG 6 cut(s) 297, 337, 594, 1283, 1391, 1781
Bse1I ACTGG 6 cut(s) 976, 1633, 1697, 1702, 2171, 2232
Bse3DI GCAATG 2 cut(s) 814, 2019
BseBI CCWGG 6 cut(s) 595, 731, 1197, 1283, 1299, 2127
BseDI CCNNGG 5 cut(s) 126, 613, 1195, 1282, 1491
BseGI GGATG 6 cut(s) 255, 382, 564, 1090, 1327, 2389
BseLI CCNNNNNNNGG 6 cut(s) 297, 337, 594, 1283, 1391, 1781
BseMI GCAATG 2 cut(s) 814, 2019
BseMII CTCAG 4 cut(s) 1893, 2093, 2208, 2634
BseNI ACTGG 6 cut(s) 976, 1633, 1697, 1702, 2171, 2232
BseRI GAGGAG 3 cut(s) 565, 568, 2424
BseSI GKGCMC 1 cut(s) 2015
BseXI GCAGC 6 cut(s) 130, 783, 1696, 1915, 2324, 2361
BseYI CCCAGC 1 cut(s) 152
BshNI GGYRCC 2 cut(s) 770, 2012
BsiHKAI GWGCWC 7 cut(s) 624, 930, 1185, 1827, 1908, 2220, 2366
BsiSI CCGG 5 cut(s) 774, 899, 1491, 1526, 2367
BslFI GGGAC 4 cut(s) 700, 1488, 1931, 2628
BslI CCNNNNNNNGG 6 cut(s) 297, 337, 594, 1283, 1391, 1781
BsmAI GTCTC 1 cut(s) 1695
BsmFI GGGAC 4 cut(s) 700, 1488, 1931, 2628
Bsp119I TTCGAA 2 cut(s) 310, 2331
Bsp1286I GDGCHC 8 cut(s) 624, 930, 1185, 1827, 1908, 2015, 2220, 2366
BspACI CCGC 6 cut(s) 364, 481, 831, 1041, 1834, 2611
BspCNI CTCAG 4 cut(s) 1892, 2092, 2207, 2633
BspLI GGNNCC 8 cut(s) 715, 716, 772, 1194, 1225, 1496, 1852, 2014
BspMAI CTGCAG 3 cut(s) 148, 1832, 2161
BspPI GGATC 2 cut(s) 1103, 2528
BspQI GCTCTTC 1 cut(s) 1815
BspT104I TTCGAA 2 cut(s) 310, 2331
BspT107I GGYRCC 2 cut(s) 770, 2012
BsrDI GCAATG 2 cut(s) 814, 2019
BsrI ACTGG 6 cut(s) 976, 1633, 1697, 1702, 2171, 2232
BssECI CCNNGG 5 cut(s) 126, 613, 1195, 1282, 1491
BssNAI GTATAC 1 cut(s) 921
BssNI GRCGYC 4 cut(s) 498, 510, 735, 771
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 126
Bst1107I GTATAC 1 cut(s) 921
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 6 cut(s) 595, 731, 1197, 1283, 1299, 2127
Bst4CI ACNGT 7 cut(s) 101, 475, 1517, 1522, 2447, 2485, 2665
Bst6I CTCTTC 4 cut(s) 62, 1769, 1815, 2183
BstACI GRCGYC 4 cut(s) 498, 510, 735, 771
BstBAI YACGTR 1 cut(s) 1680
BstBI TTCGAA 2 cut(s) 310, 2331
BstC8I GCNNGC 8 cut(s) 280, 620, 685, 1027, 1440, 1832, 1955, 2635
BstDEI CTNAG 7 cut(s) 302, 1615, 1879, 2079, 2194, 2592, 2620
BstEII GGTNACC 1 cut(s) 475
BstF5I GGATG 6 cut(s) 255, 382, 564, 1090, 1327, 2389
BstH2I RGCGCY 3 cut(s) 690, 774, 1538
BstHHI GCGC 4 cut(s) 689, 773, 1537, 1959
BstMAI GTCTC 1 cut(s) 1695
BstMWI GCNNNNNNNGC 7 cut(s) 149, 680, 770, 1831, 1934, 1954, 2643
BstNI CCWGG 6 cut(s) 595, 731, 1197, 1283, 1299, 2127
BstNSI RCATGY 1 cut(s) 2261
BstPI GGTNACC 1 cut(s) 475
BstSCI CCNGG 7 cut(s) 593, 729, 1195, 1281, 1297, 1490, 2125
BstSFI CTRYAG 3 cut(s) 144, 1828, 2157
BstSLI GKGCMC 1 cut(s) 2015
BstSNI TACGTA 1 cut(s) 1680
BstV1I GCAGC 6 cut(s) 130, 783, 1696, 1915, 2324, 2361
BstV2I GAAGAC 2 cut(s) 430, 541
BstZ17I GTATAC 1 cut(s) 921
BsuI GTATCC 1 cut(s) 155
BtrI CACGTC 1 cut(s) 1434
BtsCI GGATG 6 cut(s) 255, 382, 564, 1090, 1327, 2389
BtsI GCAGTG 2 cut(s) 678, 2359
BtsIMutI CAGTG 7 cut(s) 97, 678, 983, 1210, 1690, 2225, 2359
Cac8I GCNNGC 8 cut(s) 280, 620, 685, 1027, 1440, 1832, 1955, 2635
CaiI CAGNNNCTG 2 cut(s) 152, 2086
CfoI GCGC 4 cut(s) 689, 773, 1537, 1959
CseI GACGC 2 cut(s) 518, 1490
Csp6I GTAC 4 cut(s) 1518, 1677, 1681, 2255
CviAII CATG 4 cut(s) 785, 814, 1081, 2258
CviQI GTAC 4 cut(s) 1518, 1677, 1681, 2255
DdeI CTNAG 7 cut(s) 302, 1615, 1879, 2079, 2194, 2592, 2620
DinI GGCGCC 1 cut(s) 772
DraIII CACNNNGTG 2 cut(s) 418, 1337
DriI GACNNNNNGTC 1 cut(s) 739
Eam1104I CTCTTC 4 cut(s) 62, 1769, 1815, 2183
Eam1105I GACNNNNNGTC 1 cut(s) 739
EarI CTCTTC 4 cut(s) 62, 1769, 1815, 2183
Ecl136II GAGCTC 3 cut(s) 928, 1825, 1906
Eco105I TACGTA 1 cut(s) 1680
Eco130I CCWWGG 1 cut(s) 126
Eco147I AGGCCT 2 cut(s) 1613, 2297
Eco24I GRGCYC 3 cut(s) 930, 1827, 1908
Eco32I GATATC 2 cut(s) 2056, 2626
Eco47I GGWCC 6 cut(s) 248, 556, 597, 714, 1192, 1495
Eco47III AGCGCT 1 cut(s) 1536
Eco53kI GAGCTC 3 cut(s) 928, 1825, 1906
Eco57I CTGAAG 1 cut(s) 1839
Eco91I GGTNACC 1 cut(s) 475
EcoICRI GAGCTC 3 cut(s) 928, 1825, 1906
EcoO109I RGGNCCY 4 cut(s) 597, 714, 1495, 1851
EcoO65I GGTNACC 1 cut(s) 475
EcoRI GAATTC 2 cut(s) 306, 1272
EcoRII CCWGG 6 cut(s) 593, 729, 1195, 1281, 1297, 2125
EcoRV GATATC 2 cut(s) 2056, 2626
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 3 cut(s) 1090, 2149, 2581
EcoT38I GRGCYC 3 cut(s) 930, 1827, 1908
EgeI GGCGCC 1 cut(s) 772
EheI GGCGCC 1 cut(s) 772
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 4 cut(s) 788, 817, 1084, 2261
FaqI GGGAC 4 cut(s) 700, 1488, 1931, 2628
FatI CATG 4 cut(s) 784, 813, 1080, 2257
FauI CCCGC 3 cut(s) 371, 488, 2604
FbaI TGATCA 2 cut(s) 1168, 2242
FblI GTMKAC 1 cut(s) 920
Fnu4HI GCNGC 7 cut(s) 144, 797, 1041, 1710, 1929, 2313, 2350
FokI GGATG 6 cut(s) 242, 369, 551, 1097, 1314, 2396
FriOI GRGCYC 3 cut(s) 930, 1827, 1908
Fsp4HI GCNGC 7 cut(s) 144, 797, 1041, 1710, 1929, 2313, 2350
FspBI CTAG 2 cut(s) 1653, 1847
GlaI GCGC 4 cut(s) 688, 772, 1536, 1958
GluI GCNGC 7 cut(s) 144, 797, 1041, 1710, 1929, 2313, 2350
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 2 cut(s) 735, 1719
HaeII RGCGCY 3 cut(s) 690, 774, 1538
HapII CCGG 5 cut(s) 774, 899, 1491, 1526, 2367
HgaI GACGC 2 cut(s) 518, 1490
HhaI GCGC 4 cut(s) 689, 773, 1537, 1959
Hin1I GRCGYC 4 cut(s) 498, 510, 735, 771
Hin1II CATG 4 cut(s) 788, 817, 1084, 2261
Hin6I GCGC 4 cut(s) 687, 771, 1535, 1957
HinP1I GCGC 4 cut(s) 687, 771, 1535, 1957
HincII GTYRAC 2 cut(s) 181, 1150
HindII GTYRAC 2 cut(s) 181, 1150
HinfI GANTC 8 cut(s) 188, 320, 969, 1584, 1800, 1840, 1919, 2655
HpaII CCGG 5 cut(s) 774, 899, 1491, 1526, 2367
HphI GGTGA 5 cut(s) 469, 634, 845, 1151, 2103
Hpy166II GTNNAC 7 cut(s) 181, 921, 1065, 1150, 1683, 1890, 2357
Hpy188I TCNGA 9 cut(s) 1006, 1173, 1752, 1769, 1776, 1882, 2060, 2088, 2623
Hpy8I GTNNAC 7 cut(s) 181, 921, 1065, 1150, 1683, 1890, 2357
Hpy99I CGWCG 3 cut(s) 500, 512, 1438
HpyAV CCTTC 8 cut(s) 281, 550, 643, 733, 1166, 1936, 2261, 2564
HpyCH4III ACNGT 7 cut(s) 101, 475, 1517, 1522, 2447, 2485, 2665
HpyCH4IV ACGT 7 cut(s) 498, 735, 1264, 1433, 1679, 2220, 2253
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 680, 770, 1831, 1934, 1954, 2643
HpyF3I CTNAG 7 cut(s) 302, 1615, 1879, 2079, 2194, 2592, 2620
HpySE526I ACGT 7 cut(s) 498, 735, 1264, 1433, 1679, 2220, 2253
Hsp92I GRCGYC 4 cut(s) 498, 510, 735, 771
Hsp92II CATG 4 cut(s) 788, 817, 1084, 2261
HspAI GCGC 4 cut(s) 687, 771, 1535, 1957
KasI GGCGCC 1 cut(s) 770
KflI GGGWCCC 1 cut(s) 714
Ksp22I TGATCA 2 cut(s) 1168, 2242
LguI GCTCTTC 1 cut(s) 1815
LmnI GCTCC 6 cut(s) 913, 1784, 1974, 2597, 2696, 2713
Lsp1109I GCAGC 6 cut(s) 130, 783, 1696, 1915, 2324, 2361
LweI GCATC 6 cut(s) 391, 1075, 1366, 1538, 1555, 2134
MaeI CTAG 2 cut(s) 1653, 1847
MaeII ACGT 7 cut(s) 498, 735, 1264, 1433, 1679, 2220, 2253
MaeIII GTNAC 8 cut(s) 475, 989, 1265, 1547, 1624, 2109, 2221, 2533
MhlI GDGCHC 8 cut(s) 624, 930, 1185, 1827, 1908, 2015, 2220, 2366
Mly113I GGCGCC 1 cut(s) 771
MlyI GAGTC 3 cut(s) 314, 1578, 1913
MmeI TCCRAC 3 cut(s) 450, 516, 1762
Mph1103I ATGCAT 3 cut(s) 1090, 2149, 2581
MseI TTAA 4 cut(s) 1482, 2172, 2507, 2706
MslI CAYNNNNRTG 3 cut(s) 391, 1164, 1988
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 5 cut(s) 774, 899, 1491, 1526, 2367
MspR9I CCNGG 7 cut(s) 595, 731, 1197, 1283, 1299, 1492, 2127
MvaI CCWGG 6 cut(s) 595, 731, 1197, 1283, 1299, 2127
MwoI GCNNNNNNNGC 7 cut(s) 149, 680, 770, 1831, 1934, 1954, 2643
NarI GGCGCC 1 cut(s) 771
NciI CCSGG 1 cut(s) 1492
NlaIII CATG 4 cut(s) 788, 817, 1084, 2261
NlaIV GGNNCC 8 cut(s) 715, 716, 772, 1194, 1225, 1496, 1852, 2014
NmeAIII GCCGAG 1 cut(s) 594
NmuCI GTSAC 4 cut(s) 475, 1265, 1624, 2109
NsiI ATGCAT 3 cut(s) 1090, 2149, 2581
NspI RCATGY 1 cut(s) 2261
NspV TTCGAA 2 cut(s) 310, 2331
PceI AGGCCT 2 cut(s) 1613, 2297
PciI ACATGT 1 cut(s) 2257
PciSI GCTCTTC 1 cut(s) 1815
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PfeI GAWTC 5 cut(s) 188, 969, 1800, 1840, 2655
PflFI GACNNNGTC 1 cut(s) 434
PkrI GCNGC 7 cut(s) 145, 798, 1042, 1711, 1930, 2314, 2351
PleI GAGTC 3 cut(s) 314, 1578, 1913
PluTI GGCGCC 1 cut(s) 774
PpsI GAGTC 3 cut(s) 314, 1578, 1913
Ppu21I YACGTR 1 cut(s) 1680
PpuMI RGGWCCY 3 cut(s) 597, 714, 1495
PscI ACATGT 1 cut(s) 2257
PsiI TTATAA 2 cut(s) 849, 1259
Psp124BI GAGCTC 3 cut(s) 930, 1827, 1908
Psp5II RGGWCCY 3 cut(s) 597, 714, 1495
Psp6I CCWGG 6 cut(s) 593, 729, 1195, 1281, 1297, 2125
PspEI GGTNACC 1 cut(s) 475
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 6 cut(s) 593, 729, 1195, 1281, 1297, 2125
PspN4I GGNNCC 8 cut(s) 715, 716, 772, 1194, 1225, 1496, 1852, 2014
PspPPI RGGWCCY 3 cut(s) 597, 714, 1495
PstI CTGCAG 3 cut(s) 148, 1832, 2161
PstNI CAGNNNCTG 2 cut(s) 152, 2086
PsyI GACNNNGTC 1 cut(s) 434
PvuII CAGCTG 1 cut(s) 282
RsaI GTAC 4 cut(s) 1519, 1678, 1682, 2256
RsaNI GTAC 4 cut(s) 1518, 1677, 1681, 2255
RseI CAYNNNNRTG 3 cut(s) 391, 1164, 1988
SacI GAGCTC 3 cut(s) 930, 1827, 1908
SapI GCTCTTC 1 cut(s) 1815
SaqAI TTAA 4 cut(s) 1482, 2172, 2507, 2706
SatI GCNGC 7 cut(s) 144, 797, 1041, 1710, 1929, 2313, 2350
SbfI CCTGCAGG 1 cut(s) 2161
ScaI AGTACT 1 cut(s) 1519
SchI GAGTC 3 cut(s) 314, 1578, 1913
ScrFI CCNGG 7 cut(s) 595, 731, 1197, 1283, 1299, 1492, 2127
SdaI CCTGCAGG 1 cut(s) 2161
SduI GDGCHC 8 cut(s) 624, 930, 1185, 1827, 1908, 2015, 2220, 2366
SfaNI GCATC 6 cut(s) 391, 1075, 1366, 1538, 1555, 2134
SfcI CTRYAG 3 cut(s) 144, 1828, 2157
SfoI GGCGCC 1 cut(s) 772
SfuI TTCGAA 2 cut(s) 310, 2331
SinI GGWCC 6 cut(s) 248, 556, 597, 714, 1192, 1495
SmiMI CAYNNNNRTG 3 cut(s) 391, 1164, 1988
SmlI CTYRAG 2 cut(s) 1010, 2049
SmoI CTYRAG 2 cut(s) 1010, 2049
SnaBI TACGTA 1 cut(s) 1680
Sse8387I CCTGCAGG 1 cut(s) 2161
SseBI AGGCCT 2 cut(s) 1613, 2297
SsiI CCGC 6 cut(s) 364, 481, 831, 1041, 1834, 2611
SspDI GGCGCC 1 cut(s) 770
SspI AATATT 1 cut(s) 1368
SspMI CTAG 2 cut(s) 1653, 1847
SstI GAGCTC 3 cut(s) 930, 1827, 1908
StuI AGGCCT 2 cut(s) 1613, 2297
StyD4I CCNGG 7 cut(s) 593, 729, 1195, 1281, 1297, 1490, 2125
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 7 cut(s) 101, 475, 1517, 1522, 2447, 2485, 2665
TaiI ACGT 7 cut(s) 501, 738, 1267, 1436, 1682, 2223, 2256
TaqI TCGA 5 cut(s) 310, 507, 982, 1389, 2331
TatI WGTACW 1 cut(s) 1517
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 5 cut(s) 188, 969, 1800, 1840, 2655
Tru1I TTAA 4 cut(s) 1482, 2172, 2507, 2706
Tru9I TTAA 4 cut(s) 1482, 2172, 2507, 2706
TscAI CASTG 7 cut(s) 104, 685, 983, 1210, 1697, 2232, 2359
TseFI GTSAC 4 cut(s) 475, 1265, 1624, 2109
TseI GCWGC 6 cut(s) 143, 796, 1709, 1928, 2312, 2349
Tsp45I GTSAC 4 cut(s) 475, 1265, 1624, 2109
TspDTI ATGAA 9 cut(s) 17, 23, 102, 201, 1047, 1151, 1417, 1424, 2647
TspGWI ACGGA 3 cut(s) 261, 1284, 1886
TspRI CASTG 7 cut(s) 104, 685, 983, 1210, 1697, 2232, 2359
Tth111I GACNNNGTC 1 cut(s) 434
VpaK11BI GGWCC 6 cut(s) 248, 556, 597, 714, 1192, 1495
XapI RAATTY 6 cut(s) 168, 306, 1142, 1272, 1344, 1753
XbaI TCTAGA 1 cut(s) 1652
XceI RCATGY 1 cut(s) 2261
XcmI CCANNNNNNNNNTGG 1 cut(s) 2545
XmiI GTMKAC 1 cut(s) 920
XspI CTAG 2 cut(s) 1653, 1847
ZraI GACGTC 2 cut(s) 499, 736
ZrmI AGTACT 1 cut(s) 1519
Zsp2I ATGCAT 3 cut(s) 1090, 2149, 2581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.