Belongs to the glycosyl hydrolase 31 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 36, 2280, 2861 |
| AatII | GACGTC | 1 cut(s) | 1932 |
| Acc36I | ACCTGC | 1 cut(s) | 2380 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 968 |
| AccI | GTMKAC | 4 cut(s) | 124, 495, 3636, 4013 |
| AccII | CGCG | 1 cut(s) | 2204 |
| AccIII | TCCGGA | 3 cut(s) | 1394, 2921, 3641 |
| AcoI | YGGCCR | 2 cut(s) | 2365, 2599 |
| AcuI | CTGAAG | 5 cut(s) | 1257, 3270, 3501, 4050, 4169 |
| AcyI | GRCGYC | 2 cut(s) | 1929, 2327 |
| AdeI | CACNNNGTG | 2 cut(s) | 524, 2768 |
| AflIII | ACRYGT | 1 cut(s) | 3688 |
| AgeI | ACCGGT | 3 cut(s) | 17, 1149, 3393 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2173 |
| AjiI | CACGTC | 1 cut(s) | 2170 |
| AjnI | CCWGG | 6 cut(s) | 468, 712, 2626, 2728, 3039, 3172 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1394, 1426, 2051, 2083 |
| AleI | CACNNNNGTG | 1 cut(s) | 1379 |
| Alw21I | GWGCWC | 9 cut(s) | 117, 372, 1095, 1645, 1670, 2361, 2616, 3800, 3917 |
| Alw26I | GTCTC | 5 cut(s) | 882, 2831, 3061, 3126, 3297 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 1273, 2092, 3359, 4165 |
| Ama87I | CYCGRG | 3 cut(s) | 838, 2317, 3980 |
| Aor13HI | TCCGGA | 3 cut(s) | 1394, 2921, 3641 |
| ApaI | GGGCCC | 1 cut(s) | 80 |
| ApeKI | GCWGC | 8 cut(s) | 1115, 1141, 1309, 1496, 2675, 3140, 3359, 3743 |
| ArsI | GACNNNNNNTTYG | 8 cut(s) | 1639, 1671, 1746, 1778, 1919, 1951, 3886, 3918 |
| AsiGI | ACCGGT | 3 cut(s) | 17, 1149, 3393 |
| AspLEI | GCGC | 1 cut(s) | 2123 |
| AsuC2I | CCSGG | 6 cut(s) | 384, 486, 930, 2018, 2026, 2714 |
| AsuII | TTCGAA | 3 cut(s) | 1515, 1765, 3762 |
| AsuNHI | GCTAGC | 1 cut(s) | 2471 |
| AvaI | CYCGRG | 3 cut(s) | 838, 2317, 3980 |
| AvaII | GGWCC | 9 cut(s) | 882, 1200, 1987, 2028, 2148, 2321, 2623, 3444, 4104 |
| BaeGI | GKGCMC | 1 cut(s) | 80 |
| BalI | TGGCCA | 1 cut(s) | 2601 |
| BamHI | GGATCC | 1 cut(s) | 379 |
| BanII | GRGCYC | 6 cut(s) | 80, 117, 1095, 1670, 2361, 3917 |
| BauI | CACGAG | 2 cut(s) | 1638, 3300 |
| BbsI | GAAGAC | 2 cut(s) | 1972, 3758 |
| Bbv12I | GWGCWC | 9 cut(s) | 117, 372, 1095, 1645, 1670, 2361, 2616, 3800, 3917 |
| BbvI | GCAGC | 8 cut(s) | 1127, 1128, 1321, 1508, 2687, 3152, 3346, 3755 |
| BceAI | ACGGC | 3 cut(s) | 1899, 2056, 3514 |
| BciT130I | CCWGG | 6 cut(s) | 470, 714, 2628, 2730, 3041, 3174 |
| BciVI | GTATCC | 4 cut(s) | 242, 991, 2860, 4123 |
| BclI | TGATCA | 3 cut(s) | 1426, 3048, 3673 |
| BcnI | CCSGG | 6 cut(s) | 384, 486, 930, 2018, 2026, 2714 |
| BcoDI | GTCTC | 5 cut(s) | 882, 2831, 3061, 3126, 3297 |
| BfmI | CTRYAG | 1 cut(s) | 4122 |
| BfoI | RGCGCY | 1 cut(s) | 2124 |
| BfuAI | ACCTGC | 1 cut(s) | 2380 |
| BfuI | GTATCC | 4 cut(s) | 242, 991, 2860, 4123 |
| BisI | GCNGC | 8 cut(s) | 1116, 1142, 1310, 1497, 2676, 3141, 3360, 3744 |
| BlsI | GCNGC | 8 cut(s) | 1117, 1143, 1311, 1498, 2677, 3142, 3361, 3745 |
| Bme18I | GGWCC | 9 cut(s) | 882, 1200, 1987, 2028, 2148, 2321, 2623, 3444, 4104 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2173 |
| BmeT110I | CYCGRG | 3 cut(s) | 838, 2317, 3980 |
| BmgBI | CACGTC | 1 cut(s) | 2170 |
| BmtI | GCTAGC | 1 cut(s) | 2475 |
| BpiI | GAAGAC | 2 cut(s) | 1972, 3758 |
| BpmI | CTGGAG | 4 cut(s) | 699, 3150, 3604, 3822 |
| Bpu14I | TTCGAA | 3 cut(s) | 1515, 1765, 3762 |
| BpuEI | CTTGAG | 1 cut(s) | 218 |
| BpuMI | CCSGG | 6 cut(s) | 384, 486, 930, 2018, 2026, 2714 |
| BsaAI | YACGTR | 1 cut(s) | 367 |
| BsaHI | GRCGYC | 2 cut(s) | 1929, 2327 |
| BsaI | GGTCTC | 3 cut(s) | 2831, 3061, 3297 |
| BsaWI | WCCGGW | 9 cut(s) | 17, 884, 1149, 1394, 1553, 2183, 2921, 3393, 3641 |
| Bse118I | RCCGGY | 5 cut(s) | 17, 1017, 1149, 3261, 3393 |
| Bse3DI | GCAATG | 4 cut(s) | 496, 1549, 2112, 3450 |
| BseAI | TCCGGA | 3 cut(s) | 1394, 2921, 3641 |
| BseBI | CCWGG | 6 cut(s) | 470, 714, 2628, 2730, 3041, 3174 |
| BseMI | GCAATG | 4 cut(s) | 496, 1549, 2112, 3450 |
| BseMII | CTCAG | 2 cut(s) | 102, 1080 |
| BseRI | GAGGAG | 3 cut(s) | 1611, 2859, 3858 |
| BseSI | GKGCMC | 1 cut(s) | 80 |
| BseXI | GCAGC | 8 cut(s) | 1127, 1128, 1321, 1508, 2687, 3152, 3346, 3755 |
| BseYI | CCCAGC | 1 cut(s) | 1135 |
| BsgI | GTGCAG | 3 cut(s) | 187, 1720, 3867 |
| Bsh1236I | CGCG | 1 cut(s) | 2204 |
| BshTI | ACCGGT | 3 cut(s) | 17, 1149, 3393 |
| BsiHKAI | GWGCWC | 9 cut(s) | 117, 372, 1095, 1645, 1670, 2361, 2616, 3800, 3917 |
| BsiHKCI | CYCGRG | 3 cut(s) | 838, 2317, 3980 |
| BslFI | GGGAC | 3 cut(s) | 1213, 2134, 3457 |
| BsmAI | GTCTC | 5 cut(s) | 882, 2831, 3061, 3126, 3297 |
| BsmFI | GGGAC | 3 cut(s) | 1213, 2134, 3457 |
| BsmI | GAATGC | 1 cut(s) | 3758 |
| Bso31I | GGTCTC | 3 cut(s) | 2831, 3061, 3297 |
| BsoBI | CYCGRG | 3 cut(s) | 838, 2317, 3980 |
| Bsp119I | TTCGAA | 3 cut(s) | 1515, 1765, 3762 |
| Bsp120I | GGGCCC | 1 cut(s) | 76 |
| Bsp13I | TCCGGA | 3 cut(s) | 1394, 2921, 3641 |
| Bsp1407I | TGTACA | 3 cut(s) | 511, 2755, 3685 |
| BspCNI | CTCAG | 2 cut(s) | 103, 1079 |
| BspEI | TCCGGA | 3 cut(s) | 1394, 2921, 3641 |
| BspFNI | CGCG | 1 cut(s) | 2204 |
| BspMI | ACCTGC | 1 cut(s) | 2380 |
| BspOI | GCTAGC | 1 cut(s) | 2475 |
| BspT104I | TTCGAA | 3 cut(s) | 1515, 1765, 3762 |
| BspTNI | GGTCTC | 3 cut(s) | 2831, 3061, 3297 |
| BsrDI | GCAATG | 4 cut(s) | 496, 1549, 2112, 3450 |
| BsrFI | RCCGGY | 5 cut(s) | 17, 1017, 1149, 3261, 3393 |
| BsrGI | TGTACA | 3 cut(s) | 511, 2755, 3685 |
| BssAI | RCCGGY | 5 cut(s) | 17, 1017, 1149, 3261, 3393 |
| BssNAI | GTATAC | 1 cut(s) | 4014 |
| BssNI | GRCGYC | 2 cut(s) | 1929, 2327 |
| BssSI | CACGAG | 2 cut(s) | 1638, 3300 |
| BssT1I | CCWWGG | 2 cut(s) | 1732, 3949 |
| Bst1107I | GTATAC | 1 cut(s) | 4014 |
| Bst2BI | CACGAG | 2 cut(s) | 1638, 3300 |
| Bst2UI | CCWGG | 6 cut(s) | 470, 714, 2628, 2730, 3041, 3174 |
| Bst6I | CTCTTC | 3 cut(s) | 1370, 3200, 3614 |
| BstACI | GRCGYC | 2 cut(s) | 1929, 2327 |
| BstAUI | TGTACA | 3 cut(s) | 511, 2755, 3685 |
| BstBAI | YACGTR | 1 cut(s) | 367 |
| BstBI | TTCGAA | 3 cut(s) | 1515, 1765, 3762 |
| BstC8I | GCNNGC | 5 cut(s) | 214, 1019, 2119, 2473, 3263 |
| BstDEI | CTNAG | 4 cut(s) | 111, 698, 1066, 1757 |
| BstDSI | CCRYGG | 1 cut(s) | 2202 |
| BstEII | GGTNACC | 1 cut(s) | 2175 |
| BstFNI | CGCG | 1 cut(s) | 2204 |
| BstH2I | RGCGCY | 1 cut(s) | 2124 |
| BstHHI | GCGC | 1 cut(s) | 2123 |
| BstMAI | GTCTC | 5 cut(s) | 882, 2831, 3061, 3126, 3297 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 218, 1121, 1141, 2114, 2462, 3365, 3795 |
| BstNI | CCWGG | 6 cut(s) | 470, 714, 2628, 2730, 3041, 3174 |
| BstNSI | RCATGY | 2 cut(s) | 1445, 3692 |
| BstPI | GGTNACC | 1 cut(s) | 2175 |
| BstSFI | CTRYAG | 1 cut(s) | 4122 |
| BstSLI | GKGCMC | 1 cut(s) | 80 |
| BstUI | CGCG | 1 cut(s) | 2204 |
| BstV1I | GCAGC | 8 cut(s) | 1127, 1128, 1321, 1508, 2687, 3152, 3346, 3755 |
| BstV2I | GAAGAC | 2 cut(s) | 1972, 3758 |
| BstX2I | RGATCY | 1 cut(s) | 379 |
| BstYI | RGATCY | 1 cut(s) | 379 |
| BstZ17I | GTATAC | 1 cut(s) | 4014 |
| BsuI | GTATCC | 4 cut(s) | 242, 991, 2860, 4123 |
| BtgI | CCRYGG | 1 cut(s) | 2202 |
| BtgZI | GCGATG | 1 cut(s) | 3280 |
| BtrI | CACGTC | 1 cut(s) | 2170 |
| BveI | ACCTGC | 1 cut(s) | 2380 |
| Cac8I | GCNNGC | 5 cut(s) | 214, 1019, 2119, 2473, 3263 |
| CaiI | CAGNNNCTG | 4 cut(s) | 1273, 2092, 3359, 4165 |
| CfoI | GCGC | 1 cut(s) | 2123 |
| Cfr10I | RCCGGY | 5 cut(s) | 17, 1017, 1149, 3261, 3393 |
| Cfr42I | CCGCGG | 1 cut(s) | 2205 |
| CseI | GACGC | 2 cut(s) | 2335, 2921 |
| CspAI | ACCGGT | 3 cut(s) | 17, 1149, 3393 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1876, 1911 |
| CviAII | CATG | 9 cut(s) | 268, 1442, 2164, 2216, 2245, 2380, 2512, 3689, 3695 |
| DdeI | CTNAG | 4 cut(s) | 111, 698, 1066, 1757 |
| DraIII | CACNNNGTG | 2 cut(s) | 524, 2768 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2173 |
| EaeI | YGGCCR | 2 cut(s) | 2365, 2599 |
| Eam1104I | CTCTTC | 3 cut(s) | 1370, 3200, 3614 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2173 |
| EarI | CTCTTC | 3 cut(s) | 1370, 3200, 3614 |
| EciI | GGCGGA | 2 cut(s) | 63, 3317 |
| Ecl136II | GAGCTC | 5 cut(s) | 115, 1093, 1668, 2359, 3915 |
| Eco130I | CCWWGG | 2 cut(s) | 1732, 3949 |
| Eco147I | AGGCCT | 4 cut(s) | 800, 1481, 3384, 3728 |
| Eco24I | GRGCYC | 6 cut(s) | 80, 117, 1095, 1670, 2361, 3917 |
| Eco31I | GGTCTC | 3 cut(s) | 2831, 3061, 3297 |
| Eco47I | GGWCC | 9 cut(s) | 882, 1200, 1987, 2028, 2148, 2321, 2623, 3444, 4104 |
| Eco53kI | GAGCTC | 5 cut(s) | 115, 1093, 1668, 2359, 3915 |
| Eco57I | CTGAAG | 5 cut(s) | 1257, 3270, 3501, 4050, 4169 |
| Eco88I | CYCGRG | 3 cut(s) | 838, 2317, 3980 |
| Eco91I | GGTNACC | 1 cut(s) | 2175 |
| EcoICRI | GAGCTC | 5 cut(s) | 115, 1093, 1668, 2359, 3915 |
| EcoO109I | RGGNCCY | 6 cut(s) | 1038, 1200, 2148, 3282, 3444, 3459 |
| EcoO65I | GGTNACC | 1 cut(s) | 2175 |
| EcoRI | GAATTC | 4 cut(s) | 637, 1075, 1688, 1761 |
| EcoRII | CCWGG | 6 cut(s) | 468, 712, 2626, 2728, 3039, 3172 |
| EcoT14I | CCWWGG | 2 cut(s) | 1732, 3949 |
| EcoT22I | ATGCAT | 1 cut(s) | 3580 |
| EcoT38I | GRGCYC | 6 cut(s) | 80, 117, 1095, 1670, 2361, 3917 |
| ErhI | CCWWGG | 2 cut(s) | 1732, 3949 |
| FaeI | CATG | 9 cut(s) | 271, 1445, 2167, 2219, 2248, 2383, 2515, 3692, 3698 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 4055, 4087 |
| FaqI | GGGAC | 3 cut(s) | 1213, 2134, 3457 |
| FatI | CATG | 9 cut(s) | 267, 1441, 2163, 2215, 2244, 2379, 2511, 3688, 3694 |
| FauI | CCCGC | 3 cut(s) | 1919, 1963, 2197 |
| FbaI | TGATCA | 3 cut(s) | 1426, 3048, 3673 |
| FblI | GTMKAC | 4 cut(s) | 124, 495, 3636, 4013 |
| Fnu4HI | GCNGC | 8 cut(s) | 1116, 1142, 1310, 1497, 2676, 3141, 3360, 3744 |
| FriOI | GRGCYC | 6 cut(s) | 80, 117, 1095, 1670, 2361, 3917 |
| Fsp4HI | GCNGC | 8 cut(s) | 1116, 1142, 1310, 1497, 2676, 3141, 3360, 3744 |
| GlaI | GCGC | 1 cut(s) | 2122 |
| GluI | GCNGC | 8 cut(s) | 1116, 1142, 1310, 1497, 2676, 3141, 3360, 3744 |
| GsaI | CCCAGC | 1 cut(s) | 1139 |
| GsuI | CTGGAG | 4 cut(s) | 699, 3150, 3604, 3822 |
| HaeII | RGCGCY | 1 cut(s) | 2124 |
| HgaI | GACGC | 2 cut(s) | 2335, 2921 |
| HhaI | GCGC | 1 cut(s) | 2123 |
| Hin1I | GRCGYC | 2 cut(s) | 1929, 2327 |
| Hin1II | CATG | 9 cut(s) | 271, 1445, 2167, 2219, 2248, 2383, 2515, 3692, 3698 |
| Hin6I | GCGC | 1 cut(s) | 2121 |
| HinP1I | GCGC | 1 cut(s) | 2121 |
| HincII | GTYRAC | 2 cut(s) | 125, 304 |
| HindII | GTYRAC | 2 cut(s) | 125, 304 |
| Hpy99I | CGWCG | 3 cut(s) | 1931, 1934, 2329 |
| HpyCH4IV | ACGT | 7 cut(s) | 366, 1409, 1437, 1929, 2169, 2695, 3656 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 218, 1121, 1141, 2114, 2462, 3365, 3795 |
| HpyF3I | CTNAG | 4 cut(s) | 111, 698, 1066, 1757 |
| HpySE526I | ACGT | 7 cut(s) | 366, 1409, 1437, 1929, 2169, 2695, 3656 |
| Hsp92I | GRCGYC | 2 cut(s) | 1929, 2327 |
| Hsp92II | CATG | 9 cut(s) | 271, 1445, 2167, 2219, 2248, 2383, 2515, 3692, 3698 |
| HspAI | GCGC | 1 cut(s) | 2121 |
| KflI | GGGWCCC | 3 cut(s) | 1200, 2148, 3444 |
| Kpn2I | TCCGGA | 3 cut(s) | 1394, 2921, 3641 |
| KroI | GCCGGC | 2 cut(s) | 1017, 3261 |
| KroNI | GCCGGC | 2 cut(s) | 1019, 3263 |
| Ksp22I | TGATCA | 3 cut(s) | 1426, 3048, 3673 |
| KspI | CCGCGG | 1 cut(s) | 2205 |
| Lsp1109I | GCAGC | 8 cut(s) | 1127, 1128, 1321, 1508, 2687, 3152, 3346, 3755 |
| MaeII | ACGT | 7 cut(s) | 366, 1409, 1437, 1929, 2169, 2695, 3656 |
| MfeI | CAATTG | 1 cut(s) | 1206 |
| MflI | RGATCY | 1 cut(s) | 379 |
| MlsI | TGGCCA | 1 cut(s) | 2601 |
| MluNI | TGGCCA | 1 cut(s) | 2601 |
| MlyI | GAGTC | 6 cut(s) | 942, 1769, 1819, 2934, 3186, 3632 |
| Mox20I | TGGCCA | 1 cut(s) | 2601 |
| Mph1103I | ATGCAT | 1 cut(s) | 3580 |
| MroI | TCCGGA | 3 cut(s) | 1394, 2921, 3641 |
| MroNI | GCCGGC | 2 cut(s) | 1017, 3261 |
| MscI | TGGCCA | 1 cut(s) | 2601 |
| MseI | TTAA | 5 cut(s) | 549, 3573, 3944, 3996, 4062 |
| MslI | CAYNNNNRTG | 9 cut(s) | 1175, 1379, 1436, 2168, 2595, 3419, 3681, 3693, 4080 |
| Msp20I | TGGCCA | 1 cut(s) | 2601 |
| MspA1I | CMGCKG | 1 cut(s) | 2204 |
| MunI | CAATTG | 1 cut(s) | 1206 |
| Mva1269I | GAATGC | 1 cut(s) | 3758 |
| MvaI | CCWGG | 6 cut(s) | 470, 714, 2628, 2730, 3041, 3174 |
| MvnI | CGCG | 1 cut(s) | 2204 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 218, 1121, 1141, 2114, 2462, 3365, 3795 |
| NaeI | GCCGGC | 2 cut(s) | 1019, 3263 |
| NciI | CCSGG | 6 cut(s) | 384, 486, 930, 2018, 2026, 2714 |
| NgoMIV | GCCGGC | 2 cut(s) | 1017, 3261 |
| NheI | GCTAGC | 1 cut(s) | 2471 |
| NlaIII | CATG | 9 cut(s) | 271, 1445, 2167, 2219, 2248, 2383, 2515, 3692, 3698 |
| NmuCI | GTSAC | 8 cut(s) | 20, 1528, 1906, 2175, 2186, 2264, 3152, 3540 |
| NsiI | ATGCAT | 1 cut(s) | 3580 |
| NspI | RCATGY | 2 cut(s) | 1445, 3692 |
| NspV | TTCGAA | 3 cut(s) | 1515, 1765, 3762 |
| OliI | CACNNNNGTG | 1 cut(s) | 1379 |
| PaeR7I | CTCGAG | 1 cut(s) | 838 |
| PceI | AGGCCT | 4 cut(s) | 800, 1481, 3384, 3728 |
| PciI | ACATGT | 1 cut(s) | 3688 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1935 |
| PctI | GAATGC | 1 cut(s) | 3758 |
| PdiI | GCCGGC | 2 cut(s) | 1019, 3263 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 968 |
| PfoI | TCCNGGA | 2 cut(s) | 712, 2016 |
| PinAI | ACCGGT | 3 cut(s) | 17, 1149, 3393 |
| PkrI | GCNGC | 8 cut(s) | 1117, 1143, 1311, 1498, 2677, 3142, 3361, 3745 |
| PleI | GAGTC | 6 cut(s) | 942, 1769, 1819, 2933, 3186, 3632 |
| PpsI | GAGTC | 6 cut(s) | 942, 1769, 1819, 2933, 3186, 3632 |
| Ppu21I | YACGTR | 1 cut(s) | 367 |
| PpuMI | RGGWCCY | 3 cut(s) | 1200, 2148, 3444 |
| PscI | ACATGT | 1 cut(s) | 3688 |
| PsiI | TTATAA | 3 cut(s) | 36, 2280, 2861 |
| Psp124BI | GAGCTC | 5 cut(s) | 117, 1095, 1670, 2361, 3917 |
| Psp5II | RGGWCCY | 3 cut(s) | 1200, 2148, 3444 |
| Psp6I | CCWGG | 6 cut(s) | 468, 712, 2626, 2728, 3039, 3172 |
| PspEI | GGTNACC | 1 cut(s) | 2175 |
| PspFI | CCCAGC | 1 cut(s) | 1135 |
| PspGI | CCWGG | 6 cut(s) | 468, 712, 2626, 2728, 3039, 3172 |
| PspOMI | GGGCCC | 1 cut(s) | 76 |
| PspPPI | RGGWCCY | 3 cut(s) | 1200, 2148, 3444 |
| PstNI | CAGNNNCTG | 4 cut(s) | 1273, 2092, 3359, 4165 |
| PsuI | RGATCY | 1 cut(s) | 379 |
| RseI | CAYNNNNRTG | 9 cut(s) | 1175, 1379, 1436, 2168, 2595, 3419, 3681, 3693, 4080 |
| SacI | GAGCTC | 5 cut(s) | 117, 1095, 1670, 2361, 3917 |
| SacII | CCGCGG | 1 cut(s) | 2205 |
| SalI | GTCGAC | 1 cut(s) | 123 |
| SaqAI | TTAA | 5 cut(s) | 549, 3573, 3944, 3996, 4062 |
| SatI | GCNGC | 8 cut(s) | 1116, 1142, 1310, 1497, 2676, 3141, 3360, 3744 |
| SchI | GAGTC | 6 cut(s) | 942, 1769, 1819, 2934, 3186, 3632 |
| SfcI | CTRYAG | 1 cut(s) | 4122 |
| Sfr274I | CTCGAG | 1 cut(s) | 838 |
| Sfr303I | CCGCGG | 1 cut(s) | 2205 |
| SfuI | TTCGAA | 3 cut(s) | 1515, 1765, 3762 |
| SgrAI | CRCCGGYG | 1 cut(s) | 17 |
| SgrBI | CCGCGG | 1 cut(s) | 2205 |
| SinI | GGWCC | 9 cut(s) | 882, 1200, 1987, 2028, 2148, 2321, 2623, 3444, 4104 |
| SlaI | CTCGAG | 1 cut(s) | 838 |
| SmiMI | CAYNNNNRTG | 9 cut(s) | 1175, 1379, 1436, 2168, 2595, 3419, 3681, 3693, 4080 |
| SmlI | CTYRAG | 2 cut(s) | 197, 838 |
| SmoI | CTYRAG | 2 cut(s) | 197, 838 |
| SseBI | AGGCCT | 4 cut(s) | 800, 1481, 3384, 3728 |
| SstI | GAGCTC | 5 cut(s) | 117, 1095, 1670, 2361, 3917 |
| StuI | AGGCCT | 4 cut(s) | 800, 1481, 3384, 3728 |
| StyI | CCWWGG | 2 cut(s) | 1732, 3949 |
| TaiI | ACGT | 7 cut(s) | 369, 1412, 1440, 1932, 2172, 2698, 3659 |
| TatI | WGTACW | 8 cut(s) | 105, 511, 867, 1992, 2349, 2755, 3111, 3685 |
| Tru1I | TTAA | 5 cut(s) | 549, 3573, 3944, 3996, 4062 |
| Tru9I | TTAA | 5 cut(s) | 549, 3573, 3944, 3996, 4062 |
| TseFI | GTSAC | 8 cut(s) | 20, 1528, 1906, 2175, 2186, 2264, 3152, 3540 |
| TseI | GCWGC | 8 cut(s) | 1115, 1141, 1309, 1496, 2675, 3140, 3359, 3743 |
| Tsp45I | GTSAC | 8 cut(s) | 20, 1528, 1906, 2175, 2186, 2264, 3152, 3540 |
| TspGWI | ACGGA | 5 cut(s) | 269, 1787, 2205, 2369, 3377 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 968 |
| VpaK11BI | GGWCC | 9 cut(s) | 882, 1200, 1987, 2028, 2148, 2321, 2623, 3444, 4104 |
| XbaI | TCTAGA | 1 cut(s) | 559 |
| XceI | RCATGY | 2 cut(s) | 1445, 3692 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 170, 2414 |
| XhoI | CTCGAG | 1 cut(s) | 838 |
| XmiI | GTMKAC | 4 cut(s) | 124, 495, 3636, 4013 |
| ZraI | GACGTC | 1 cut(s) | 1930 |
| Zsp2I | ATGCAT | 1 cut(s) | 3580 |