Rh5DG149500

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000882
Physical Location & Seq
Reverse (-)
15164145 .. 15167641
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG149500.1

Sequence Viewer

Length: 2601 bp
ATGGAAAGAAACCGATCAAAATACACACCAAAAGCCACATTCCATCATCTTCTACCATTCTTCTTCTTCTATATCTTCTTCTCTCTTGATTGCGTAGTTGCACAAGAGTTAGTTGGGTATGGATATAAAGTCGAGTCTGTGAATAATTCTGATTCCTCGGGGAAGACATTGACCGTTAACCTTGGTCTCATCAACAGCTCCTCAGTTTATGGACCTGACATATCAAATCTTACCCTCTATGTCAGCTATGAGACCAAAGACCGTCTAAGAGTTCGAATAACAGACTCAACGCAACAAAGATGGGAGCTCCCACAAGACATTCTCCCCCGCCAAAACCAAACTTCACAGAACTTTTCCGTCACTGACAACGACCTCGTTTTCACCCTCCGCAACACCACCCCGTTCGGCTTCACCATCACCCGCCGCTCCTCTAACGACACCGTTTTCGACACCACCCCGAACCCCTCCGACCCCAACACCACCTTCATCTTCAAGGACCAATACTTCCAGCTCTCCTCCTCTCTTCCCAACAACAGGTCCTCCCTGTACGGACTCGGAGAGCACACCAAGAGCACCTTCAAGCTCCTAGCCAATCAGACTCTGACGCTCTGGAGCGCCGACATCGGCAGCGTGAGTCCCGACCTCAATCTCTATGGGTCCCACCCCTTCTACATGGACGTAAGATCGCCGTCCAGTGATGGCAAGGTCAAGGCGGGAACCACTCACGGAGTGCTGCTTTTGAACAGCAATGGTATGGATGTAAATTACACCGGTGACAGGGTTACTTATAATGTGATTGGCGGAGTTGTGGACTTGTATTTCTTTTCGGGTCCGACACCCGAATTGGTGATGGAGCAGTACACTGAGCTCATTGGTCGACCCGCCCCTATGCCATATTGGTCTTTCGGATTCCACCAGTGTAGATATGGTTACAAGGATGTTTCTGATCTTGAGGGGGTCGTTGCTGGTTATGCAAATGCTAGTATCCCTCTTGAGGTTATGTGGACCGACATTGATTACATGGATGCTTACAAGGATTTCACTCTTGATCCCGTCAACTTTCCATTAGACAGGATGAAGAACTTTACCAACAACCTTCACCAAAATGGTCAGAAATACGTGCTCATCTTGGATCCCGGTATTAGTATCAATGATAGCTATGGAACCTACACCAGAGGGAAGGAAGCAGATATATTTATAAAACGTGATGGCATTCCTTACCAGGGAAATGTTTGGCCTGGCAATGTTTACTTTCCTGATTTTGTAAATCCACAAAGTGAACCATATTGGGAGAATGAGATTAAACTATTTCTAGACCAGCTACCTGTTGATGGCCTTTGGCTTGATATGAATGAAGTATCAAATTTCCAAACTTCTAATCCCACTCCGAATTCCACACTTGATGATCCACCTTACAAAATCAACGATGCTGGAGGCCACCGTCCGATCCTTAGTAGGACTATTCCAGGATCAGCTCTACACTTTGGTAATGTTACAGAGTATAATGTTCATAACCTATTTGGCATGTTAGAAGCCAAAGCAACAAACAAGGCCTTGACCAATTTGACTGGTAAGAGACCCTTTGTTCTCTCGAGATCGACTTTTGTAAGCTCTGGTAAGTACACAGCACACTGGACCGGAGACAATGGTGCAAAATGGAGTGATTTGGCATACTCAATTCCGGGCATTTTGAATTTTGGACTCTTTGGAGTTCCAATGGTTGGAGCTGATATATGTGGTTTCTCTGGGGATACTAATGAAGAACTCTGCCGGCGTTGGATTCAGCTAGGGGCCTTTTACCCCTTTTCAAGAGATCATTCGGATAAGAATTCAATCCGGCAGGAGCTCTATGTTTGGGAATCAGTGGCTGCATCAGCAAAAAAAGTGCTGGGGCTACGTTACCGGTTGCTTCCCTTGTTCTATACATCAATGTATCAAGCACACAAGAACGGGACTCCAATTGCAAGGCCCGTATTCTTCTCATTTCCTGAAGATACCAATACATACAGTATCTATTCACAGTTTCTGATTGGTAAAGGCGTAATGGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTGAGGCATATTTTCCTGCAGGAAATTGGTTCGATCTCTTCAACTACACATCAGTTAGTGTAGACTCTGGAAAGAATGTTACGTTGGATGCACCGCCTGATCATATCAATGTGCATGTCCACGAAGGAAACATTTTGGCCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCCCGAAACACTACATTCGAACTTTTGGTGGTCACTGGTAGTAGCAATGAGAGTACCGGAGAATTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTTGTGAGGTTTCGTTCTGCACGAGCACAAAATGGAAGTCTAGTTTTGAGCTCAAAGGTCGAAAATGGAGAATTTGCATTAAGCCAAGGTTGGATCATTGATAAAATTACCGTCCTTGGGTTGGACAAAGTTAATGCAACACAGTCCAGTGTATCCCTTCCTATAGGAAAGGAGTTTGAGTTGGAGCTGAAGCCGAATCAGACACTGGCGAAACCAAAAACATATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

866

Amino Acids

96.8

Weight (kDa)

5.29

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 82 - 249 2.4e-10 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 294 - 642 7.9e-123 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 651 - 739 2.9e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 789, 1199
AccB7I CCANNNNNTGG 1 cut(s) 1721
AccBSI CCGCTC 1 cut(s) 426
AccI GTMKAC 2 cut(s) 877, 2142
AciI CCGC 8 cut(s) 328, 388, 421, 424, 713, 801, 882, 2174
AclWI GGATC 7 cut(s) 1043, 1127, 1140, 1400, 1441, 1477, 2471
AcsI RAATTY 6 cut(s) 1363, 1390, 1693, 1828, 2074, 2441
AcuI CTGAAG 2 cut(s) 2010, 2579
AdeI CACNNNGTG 2 cut(s) 730, 1277
AfaI GTAC 4 cut(s) 548, 860, 1622, 2305
AfiI CCNNNNNNNGG 8 cut(s) 534, 777, 994, 1223, 1331, 1721, 2487, 2491
AgeI ACCGGT 2 cut(s) 770, 1902
AgsI TTSAA 7 cut(s) 493, 580, 742, 1693, 1809, 1833, 2122
AjnI CCWGG 3 cut(s) 1221, 1237, 1465
AjuI GAANNNNNNNTTGG 6 cut(s) 467, 499, 560, 592, 2147, 2179
Alw21I GWGCWC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
Alw26I GTCTC 4 cut(s) 191, 245, 1570, 1635
AlwI GGATC 7 cut(s) 1043, 1127, 1140, 1400, 1441, 1477, 2471
AlwNI CAGNNNCTG 4 cut(s) 601, 1868, 2026, 2575
Ama87I CYCGRG 2 cut(s) 157, 1591
AoxI GGCC 8 cut(s) 1235, 1333, 1435, 1551, 1791, 1967, 2217, 2232
ApeKI GCWGC 5 cut(s) 627, 733, 1868, 2062, 2249
ApoI RAATTY 6 cut(s) 1363, 1390, 1693, 1828, 2074, 2441
ArsI GACNNNNNNTTYG 4 cut(s) 428, 460, 2392, 2424
AsiGI ACCGGT 2 cut(s) 770, 1902
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 9 cut(s) 212, 496, 537, 657, 830, 1005, 1635, 1791, 1968
AsuC2I CCSGG 2 cut(s) 1137, 1683
AsuHPI GGTGA 7 cut(s) 373, 403, 409, 785, 859, 1091, 2043
AsuII TTCGAA 2 cut(s) 274, 2268
AvaI CYCGRG 2 cut(s) 157, 1591
AvaII GGWCC 7 cut(s) 212, 496, 537, 657, 830, 1005, 1635
BamHI GGATCC 1 cut(s) 1132
BanII GRGCYC 4 cut(s) 309, 870, 1848, 2423
BauI CACGAG 1 cut(s) 2391
BbsI GAAGAC 1 cut(s) 170
Bbv12I GWGCWC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
BbvI GCAGC 5 cut(s) 639, 720, 1855, 2074, 2261
BccI CCATC 8 cut(s) 51, 294, 422, 692, 844, 1202, 1325, 2321
BceAI ACGGC 1 cut(s) 673
BciT130I CCWGG 3 cut(s) 1223, 1239, 1467
BciVI GTATCC 3 cut(s) 995, 1744, 2533
BclI TGATCA 1 cut(s) 2179
BcnI CCSGG 2 cut(s) 1137, 1683
BcoDI GTCTC 4 cut(s) 191, 245, 1570, 1635
BfaI CTAG 5 cut(s) 587, 981, 1313, 1787, 2411
BfmI CTRYAG 2 cut(s) 2097, 2532
BfoI RGCGCY 1 cut(s) 618
BfuI GTATCC 3 cut(s) 995, 1744, 2533
BisI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
BlsI GCNGC 6 cut(s) 425, 629, 735, 1870, 2064, 2251
Bme1390I CCNGG 5 cut(s) 1137, 1223, 1239, 1467, 1683
Bme18I GGWCC 7 cut(s) 212, 496, 537, 657, 830, 1005, 1635
BmeT110I CYCGRG 2 cut(s) 157, 1591
BmgT120I GGNCC 9 cut(s) 212, 496, 537, 657, 830, 1005, 1635, 1791, 1968
BmiI GGNNCC 7 cut(s) 658, 659, 718, 831, 1134, 1165, 1792
BmrFI CCNGG 5 cut(s) 1137, 1223, 1239, 1467, 1683
BmsI GCATC 4 cut(s) 1015, 1417, 1880, 2158
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 2 cut(s) 631, 1452
Bpu14I TTCGAA 2 cut(s) 274, 2268
BpuEI CTTGAG 2 cut(s) 971, 1013
BpuMI CCSGG 2 cut(s) 1137, 1683
BsaAI YACGTR 1 cut(s) 1120
BsaI GGTCTC 3 cut(s) 191, 245, 1570
BsaJI CCNNGG 5 cut(s) 156, 181, 1222, 2455, 2485
BsaWI WCCGGW 4 cut(s) 770, 1637, 1902, 2306
BsaXI ACNNNNNCTCC 6 cut(s) 524, 554, 2322, 2352, 2534, 2564
Bsc4I CCNNNNNNNGG 8 cut(s) 534, 777, 994, 1223, 1331, 1721, 2487, 2491
Bse118I RCCGGY 3 cut(s) 770, 1770, 1902
Bse1I ACTGG 7 cut(s) 693, 916, 1573, 1637, 2290, 2517, 2580
Bse3DI GCAATG 3 cut(s) 754, 1249, 2302
BseBI CCWGG 3 cut(s) 1223, 1239, 1467
BseDI CCNNGG 5 cut(s) 156, 181, 1222, 2455, 2485
BseGI GGATG 6 cut(s) 763, 943, 1030, 1080, 2173, 2329
BseLI CCNNNNNNNGG 8 cut(s) 534, 777, 994, 1223, 1331, 1721, 2487, 2491
BseMI GCAATG 3 cut(s) 754, 1249, 2302
BseMII CTCAG 2 cut(s) 216, 855
BseNI ACTGG 7 cut(s) 693, 916, 1573, 1637, 2290, 2517, 2580
BseRI GAGGAG 5 cut(s) 190, 418, 505, 508, 2364
BseXI GCAGC 5 cut(s) 639, 720, 1855, 2074, 2261
BseYI CCCAGC 1 cut(s) 1888
BsgI GTGCAG 1 cut(s) 2373
BshFI GGCC 8 cut(s) 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
BshTI ACCGGT 2 cut(s) 770, 1902
BsiHKAI GWGCWC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
BsiHKCI CYCGRG 2 cut(s) 157, 1591
BsiSI CCGG 8 cut(s) 771, 1137, 1638, 1682, 1771, 1837, 1903, 2307
BslFI GGGAC 3 cut(s) 621, 643, 1966
BslI CCNNNNNNNGG 8 cut(s) 534, 777, 994, 1223, 1331, 1721, 2487, 2491
BsmAI GTCTC 4 cut(s) 191, 245, 1570, 1635
BsmFI GGGAC 3 cut(s) 621, 643, 1966
BsmI GAATGC 1 cut(s) 1212
BsnI GGCC 8 cut(s) 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
Bso31I GGTCTC 3 cut(s) 191, 245, 1570
BsoBI CYCGRG 2 cut(s) 157, 1591
Bsp119I TTCGAA 2 cut(s) 274, 2268
Bsp1286I GDGCHC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
BspACI CCGC 8 cut(s) 328, 388, 421, 424, 713, 801, 882, 2174
BspANI GGCC 8 cut(s) 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
BspCNI CTCAG 2 cut(s) 215, 856
BspLI GGNNCC 7 cut(s) 658, 659, 718, 831, 1134, 1165, 1792
BspMAI CTGCAG 1 cut(s) 2101
BspPI GGATC 7 cut(s) 1043, 1127, 1140, 1400, 1441, 1477, 2471
BspT104I TTCGAA 2 cut(s) 274, 2268
BspTNI GGTCTC 3 cut(s) 191, 245, 1570
BsrBI CCGCTC 1 cut(s) 426
BsrDI GCAATG 3 cut(s) 754, 1249, 2302
BsrFI RCCGGY 3 cut(s) 770, 1770, 1902
BsrI ACTGG 7 cut(s) 693, 916, 1573, 1637, 2290, 2517, 2580
BssAI RCCGGY 3 cut(s) 770, 1770, 1902
BssECI CCNNGG 5 cut(s) 156, 181, 1222, 2455, 2485
BssSI CACGAG 1 cut(s) 2391
BssT1I CCWWGG 3 cut(s) 181, 2455, 2485
Bst2BI CACGAG 1 cut(s) 2391
Bst2UI CCWGG 3 cut(s) 1223, 1239, 1467
Bst4CI ACNGT 8 cut(s) 175, 263, 442, 1442, 2009, 2022, 2482, 2514
Bst6I CTCTTC 2 cut(s) 528, 2123
BstBAI YACGTR 1 cut(s) 1120
BstBI TTCGAA 2 cut(s) 274, 2268
BstC8I GCNNGC 1 cut(s) 1772
BstDEI CTNAG 4 cut(s) 202, 266, 864, 1451
BstF5I GGATG 6 cut(s) 763, 943, 1030, 1080, 2173, 2329
BstH2I RGCGCY 1 cut(s) 618
BstHHI GCGC 1 cut(s) 617
BstMAI GTCTC 4 cut(s) 191, 245, 1570, 1635
BstMWI GCNNNNNNNGC 2 cut(s) 971, 1874
BstNI CCWGG 3 cut(s) 1223, 1239, 1467
BstNSI RCATGY 2 cut(s) 1528, 2198
BstSCI CCNGG 5 cut(s) 1135, 1221, 1237, 1465, 1681
BstSFI CTRYAG 2 cut(s) 2097, 2532
BstV1I GCAGC 5 cut(s) 639, 720, 1855, 2074, 2261
BstV2I GAAGAC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 1132
BstYI RGATCY 1 cut(s) 1132
BsuI GTATCC 3 cut(s) 995, 1744, 2533
BsuRI GGCC 8 cut(s) 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
BtsCI GGATG 6 cut(s) 763, 943, 1030, 1080, 2173, 2329
BtsIMutI CAGTG 9 cut(s) 360, 700, 861, 923, 1630, 1869, 2283, 2524, 2573
Cac8I GCNNGC 1 cut(s) 1772
CaiI CAGNNNCTG 4 cut(s) 601, 1868, 2026, 2575
CfoI GCGC 1 cut(s) 617
Cfr10I RCCGGY 3 cut(s) 770, 1770, 1902
Cfr13I GGNCC 9 cut(s) 212, 496, 537, 657, 830, 1005, 1635, 1791, 1968
CseI GACGC 1 cut(s) 613
Csp6I GTAC 4 cut(s) 547, 859, 1621, 2304
CspAI ACCGGT 2 cut(s) 770, 1902
CviAII CATG 4 cut(s) 673, 1021, 1525, 2195
CviQI GTAC 4 cut(s) 547, 859, 1621, 2304
DdeI CTNAG 4 cut(s) 202, 266, 864, 1451
DraIII CACNNNGTG 2 cut(s) 730, 1277
Eam1104I CTCTTC 2 cut(s) 528, 2123
EarI CTCTTC 2 cut(s) 528, 2123
EciI GGCGGA 1 cut(s) 816
Ecl136II GAGCTC 4 cut(s) 307, 868, 1846, 2421
Eco130I CCWWGG 3 cut(s) 181, 2455, 2485
Eco147I AGGCCT 2 cut(s) 1553, 2234
Eco24I GRGCYC 4 cut(s) 309, 870, 1848, 2423
Eco31I GGTCTC 3 cut(s) 191, 245, 1570
Eco47I GGWCC 7 cut(s) 212, 496, 537, 657, 830, 1005, 1635
Eco53kI GAGCTC 4 cut(s) 307, 868, 1846, 2421
Eco57I CTGAAG 2 cut(s) 2010, 2579
Eco88I CYCGRG 2 cut(s) 157, 1591
EcoICRI GAGCTC 4 cut(s) 307, 868, 1846, 2421
EcoO109I RGGNCCY 3 cut(s) 537, 657, 1791
EcoRI GAATTC 2 cut(s) 1390, 1828
EcoRII CCWGG 3 cut(s) 1221, 1237, 1465
EcoT14I CCWWGG 3 cut(s) 181, 2455, 2485
EcoT38I GRGCYC 4 cut(s) 309, 870, 1848, 2423
ErhI CCWWGG 3 cut(s) 181, 2455, 2485
FaeI CATG 4 cut(s) 676, 1024, 1528, 2198
FalI AAGNNNNNCTT 4 cut(s) 560, 592, 1565, 1597
FaqI GGGAC 3 cut(s) 621, 643, 1966
FatI CATG 4 cut(s) 672, 1020, 1524, 2194
FauI CCCGC 4 cut(s) 335, 428, 706, 889
FbaI TGATCA 1 cut(s) 2179
FblI GTMKAC 2 cut(s) 877, 2142
Fnu4HI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
FokI GGATG 6 cut(s) 770, 950, 1037, 1087, 2180, 2336
FriOI GRGCYC 4 cut(s) 309, 870, 1848, 2423
Fsp4HI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
FspBI CTAG 5 cut(s) 587, 981, 1313, 1787, 2411
GlaI GCGC 1 cut(s) 616
GluI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
GsaI CCCAGC 1 cut(s) 1892
GsuI CTGGAG 2 cut(s) 631, 1452
HaeII RGCGCY 1 cut(s) 618
HaeIII GGCC 8 cut(s) 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234
HapII CCGG 8 cut(s) 771, 1137, 1638, 1682, 1771, 1837, 1903, 2307
HgaI GACGC 1 cut(s) 613
HhaI GCGC 1 cut(s) 617
Hin1II CATG 4 cut(s) 676, 1024, 1528, 2198
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HincII GTYRAC 3 cut(s) 178, 878, 1057
HindII GTYRAC 3 cut(s) 178, 878, 1057
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 8 cut(s) 771, 1137, 1638, 1682, 1771, 1837, 1903, 2307
HphI GGTGA 7 cut(s) 373, 403, 409, 785, 859, 1091, 2043
HpyAV CCTTC 7 cut(s) 493, 586, 676, 1106, 1174, 2198, 2537
HpyCH4III ACNGT 8 cut(s) 175, 263, 442, 1442, 2009, 2022, 2482, 2514
HpyCH4IV ACGT 5 cut(s) 678, 1119, 1204, 1897, 2162
HpyF10VI GCNNNNNNNGC 2 cut(s) 971, 1874
HpyF3I CTNAG 4 cut(s) 202, 266, 864, 1451
HpySE526I ACGT 5 cut(s) 678, 1119, 1204, 1897, 2162
Hsp92II CATG 4 cut(s) 676, 1024, 1528, 2198
HspAI GCGC 1 cut(s) 615
KflI GGGWCCC 1 cut(s) 657
KroI GCCGGC 1 cut(s) 1770
KroNI GCCGGC 1 cut(s) 1772
Ksp22I TGATCA 1 cut(s) 2179
KspAI GTTAAC 1 cut(s) 178
Lsp1109I GCAGC 5 cut(s) 639, 720, 1855, 2074, 2261
LweI GCATC 4 cut(s) 1015, 1417, 1880, 2158
MaeI CTAG 5 cut(s) 587, 981, 1313, 1787, 2411
MaeII ACGT 5 cut(s) 678, 1119, 1204, 1897, 2162
MaeIII GTNAC 9 cut(s) 358, 773, 781, 929, 1492, 1898, 2049, 2158, 2281
MbiI CCGCTC 1 cut(s) 426
MfeI CAATTG 1 cut(s) 1959
MflI RGATCY 1 cut(s) 1132
MhlI GDGCHC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
MlyI GAGTC 8 cut(s) 143, 278, 546, 592, 643, 1695, 1948, 2138
MmeI TCCRAC 8 cut(s) 492, 857, 1702, 1757, 2145, 2441, 2472, 2532
MroNI GCCGGC 1 cut(s) 1770
MseI TTAA 4 cut(s) 177, 1302, 2450, 2502
MslI CAYNNNNRTG 3 cut(s) 1104, 2187, 2596
MspI CCGG 8 cut(s) 771, 1137, 1638, 1682, 1771, 1837, 1903, 2307
MspR9I CCNGG 5 cut(s) 1137, 1223, 1239, 1467, 1683
MunI CAATTG 1 cut(s) 1959
Mva1269I GAATGC 1 cut(s) 1212
MvaI CCWGG 3 cut(s) 1223, 1239, 1467
MwoI GCNNNNNNNGC 2 cut(s) 971, 1874
NaeI GCCGGC 1 cut(s) 1772
NciI CCSGG 2 cut(s) 1137, 1683
NgoMIV GCCGGC 1 cut(s) 1770
NlaIII CATG 4 cut(s) 676, 1024, 1528, 2198
NlaIV GGNNCC 7 cut(s) 658, 659, 718, 831, 1134, 1165, 1792
NmuCI GTSAC 4 cut(s) 358, 773, 2049, 2281
NspI RCATGY 2 cut(s) 1528, 2198
NspV TTCGAA 2 cut(s) 274, 2268
PaeR7I CTCGAG 1 cut(s) 1591
PceI AGGCCT 2 cut(s) 1553, 2234
PcsI WCGNNNNNNNCGW 1 cut(s) 2389
PctI GAATGC 1 cut(s) 1212
PdiI GCCGGC 1 cut(s) 1772
PfeI GAWTC 5 cut(s) 152, 909, 1780, 1859, 2566
PflMI CCANNNNNTGG 1 cut(s) 1721
PfoI TCCNGGA 1 cut(s) 1465
PinAI ACCGGT 2 cut(s) 770, 1902
PkrI GCNGC 6 cut(s) 425, 629, 735, 1870, 2064, 2251
PleI GAGTC 8 cut(s) 142, 278, 546, 592, 642, 1695, 1948, 2138
PpsI GAGTC 8 cut(s) 142, 278, 546, 592, 642, 1695, 1948, 2138
Ppu21I YACGTR 1 cut(s) 1120
PpuMI RGGWCCY 2 cut(s) 537, 657
PsiI TTATAA 2 cut(s) 789, 1199
Psp124BI GAGCTC 4 cut(s) 309, 870, 1848, 2423
Psp5II RGGWCCY 2 cut(s) 537, 657
Psp6I CCWGG 3 cut(s) 1221, 1237, 1465
PspFI CCCAGC 1 cut(s) 1888
PspGI CCWGG 3 cut(s) 1221, 1237, 1465
PspN4I GGNNCC 7 cut(s) 658, 659, 718, 831, 1134, 1165, 1792
PspPI GGNCC 9 cut(s) 212, 496, 537, 657, 830, 1005, 1635, 1791, 1968
PspPPI RGGWCCY 2 cut(s) 537, 657
PstI CTGCAG 1 cut(s) 2101
PstNI CAGNNNCTG 4 cut(s) 601, 1868, 2026, 2575
PsuI RGATCY 1 cut(s) 1132
RsaI GTAC 4 cut(s) 548, 860, 1622, 2305
RsaNI GTAC 4 cut(s) 547, 859, 1621, 2304
RseI CAYNNNNRTG 3 cut(s) 1104, 2187, 2596
SacI GAGCTC 4 cut(s) 309, 870, 1848, 2423
SalI GTCGAC 1 cut(s) 876
SaqAI TTAA 4 cut(s) 177, 1302, 2450, 2502
SatI GCNGC 6 cut(s) 424, 628, 734, 1869, 2063, 2250
Sau96I GGNCC 9 cut(s) 212, 496, 537, 657, 830, 1005, 1635, 1791, 1968
SbfI CCTGCAGG 1 cut(s) 2101
SchI GAGTC 8 cut(s) 143, 278, 546, 592, 643, 1695, 1948, 2138
ScrFI CCNGG 5 cut(s) 1137, 1223, 1239, 1467, 1683
SdaI CCTGCAGG 1 cut(s) 2101
SduI GDGCHC 8 cut(s) 309, 564, 575, 870, 1125, 1848, 2398, 2423
SfaNI GCATC 4 cut(s) 1015, 1417, 1880, 2158
SfcI CTRYAG 2 cut(s) 2097, 2532
Sfr274I CTCGAG 1 cut(s) 1591
SfuI TTCGAA 2 cut(s) 274, 2268
SgrAI CRCCGGYG 1 cut(s) 770
SinI GGWCC 7 cut(s) 212, 496, 537, 657, 830, 1005, 1635
SlaI CTCGAG 1 cut(s) 1591
SmiMI CAYNNNNRTG 3 cut(s) 1104, 2187, 2596
SmlI CTYRAG 3 cut(s) 950, 992, 1591
SmoI CTYRAG 3 cut(s) 950, 992, 1591
Sse8387I CCTGCAGG 1 cut(s) 2101
SseBI AGGCCT 2 cut(s) 1553, 2234
SsiI CCGC 8 cut(s) 328, 388, 421, 424, 713, 801, 882, 2174
SspMI CTAG 5 cut(s) 587, 981, 1313, 1787, 2411
SstI GAGCTC 4 cut(s) 309, 870, 1848, 2423
StuI AGGCCT 2 cut(s) 1553, 2234
StyD4I CCNGG 5 cut(s) 1135, 1221, 1237, 1465, 1681
StyI CCWWGG 3 cut(s) 181, 2455, 2485
TaaI ACNGT 8 cut(s) 175, 263, 442, 1442, 2009, 2022, 2482, 2514
TaiI ACGT 5 cut(s) 681, 1122, 1207, 1900, 2165
TaqII GACCGA 1 cut(s) 1022
TatI WGTACW 2 cut(s) 858, 1620
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 5 cut(s) 152, 909, 1780, 1859, 2566
Tru1I TTAA 4 cut(s) 177, 1302, 2450, 2502
Tru9I TTAA 4 cut(s) 177, 1302, 2450, 2502
TscAI CASTG 9 cut(s) 367, 700, 868, 923, 1637, 1869, 2290, 2524, 2580
TseFI GTSAC 4 cut(s) 358, 773, 2049, 2281
TseI GCWGC 5 cut(s) 627, 733, 1868, 2062, 2249
Tsp45I GTSAC 4 cut(s) 358, 773, 2049, 2281
TspDTI ATGAA 6 cut(s) 475, 1091, 1364, 1368, 1499, 1773
TspGWI ACGGA 3 cut(s) 346, 564, 741
TspRI CASTG 9 cut(s) 367, 700, 868, 923, 1637, 1869, 2290, 2524, 2580
Van91I CCANNNNNTGG 1 cut(s) 1721
VpaK11BI GGWCC 7 cut(s) 212, 496, 537, 657, 830, 1005, 1635
XapI RAATTY 6 cut(s) 1363, 1390, 1693, 1828, 2074, 2441
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 2 cut(s) 1528, 2198
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XhoI CTCGAG 1 cut(s) 1591
XmiI GTMKAC 2 cut(s) 877, 2142
XspI CTAG 5 cut(s) 587, 981, 1313, 1787, 2411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.