Rh5BG135500

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000882
Physical Location & Seq
Forward (+)
13449814 .. 13463699
13886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG135500.1

Sequence Viewer

Length: 2373 bp
ATGAAAGGGTCTGATTTCAAAGCTGGAGCGAGATGCAAAAAGGAAGAACCAGTTGGGTATGGATATAAAGTTGAGTATGTGAATTCTGATTCCTCTGGGAAGACATTGACGGCTAATCTTGGCCTGATCAATAGCTCCTCAATCTATGGACCTGACATCCCAAATCTAAAGCTTTATGCCAGCTATGAAACCAAAGAAAGACTAAGAATTCGAATAACAGACTCGAGTAACCAAAGATGGGAAATCCCACAAGACCTTCTCCCTCGACAGACTCCACAAACATCCCACCATTCGATCCCCGAAAACGATCTCATTTTCACCCTCCACACCACCACCCCGTTCGGCTTCACGGTCACCCGCCGCTTCTCCAATGATGTCGTATTCGACGCCACCCCAAACCCCTCCAACCCTTCCTCCATCCTCGTCTTCAAGGACCAGTACATCCAGCTTTCCTCCTCTCTCCCGGAAACTCGATCTTCCCTGTACGGCCTCGGTGAGCACACCAAAGCCTCCTTCAAGCTCCAATCCAATCAGACTCTAACACTCTGGACCGCCGACATTTTCAGCTCCATCCCCGACCTCAATCTCTATGGGTCCCACCCATTTTACATGGACGTGAGGTCACCGTCCGGTGATGGAAAAGTTACTGCAGGAACCACTCATGGAGTTTTGCTATTGAATAGTAATGGCATGGATGTAAATTACGACGGTGACAGGATTACATATAAAGTGATTGGCGGAGTTGTTGACTTGTATTTTTTTTCGGGTCCGACGCCGGAATTGGTGATGGAGCAGTACACGGAGTTCATTGGCCGACCTGCCCCGATGCCCTACTGGTCTTTTGGATTCCACCAATGTAGATATAGTTACAAGGATGTTGCTGATCTTGAGGAGGTGGTTGCTGGTTATGCAAATGCTAGCATCCCTCTTGAAGGTATTTGGACAGACATTGACTACATGGATGCTTACAAGGATTTCACTCTTGATCCCATCAACTTTCCATTAAGTAAGATGCAGAAGTTTACAAACACCCTTCACCAAAATGGTCAGAAATATGTGCCGATCTTGGACCCTGGTATTAGTATAAATGAGAGCTATGGAAGTTACATCAGAGGGAAGGAAGCAGATATATACATAAAACGTGATGGTATTCCTTACCAGGGACATGTATGGCCTGGTGATGTTAACTATCCTGATTTTGTACATCCACAAAGTGAACAATTTTGGGCTAATGAGATCAAACTATTTCAAGACCAACTAGCTTTTGATGGTCTTTGGCTTGATATGAATGAGCCATGCAATTTGATAACATCTGCTCCCACTCCATATTCTACACTAGATGATCCACCTTACAAAATCAATGATTCCGGAGTCCAGCGTCCGACCATTAATAAGACTGTTCCGGCATCAGCTCTACACTTTGGTAATATAACAGAGTATAATGTTCATAACCTATATGGGTTCTTGGAATCTAAAGCAACACACCAGGGGTTAATCAATGTCACTGGTAAGAGACCATTTATACTCACTAGATCAACCTTTGTAAGCTCTGGTAAGTACACAGCACACTGGACTGGAGACAATGCAGCAAGATGGAGTGACTTAGCATACACAATTCCAGGCATTTTGAATTTTGGACTCTTCGGAGTTCCGATGGTTGGAGCTGATATATGTGGATTCACTTTGAATACTACTGAAGAACTCTGCAGGCGATGGATCCAGCTAGGGGCCTTTTACCCGTTTGCAAGAGATCATTCAGATATATTTACAATCCGCCAAGAGCTCTATGTTTGGGATTCAGTTGCTGCATCAGCAAGGAAGGTGCTAGGCCTCCGTTACCGGTTGCTCCCCTTGTTCTACACATCAATGTATCAGGCACACAAAAAAGGGACCCCCATTGCAAGGCCCCTTTTCTTCTCGTTTCCTGAAGATACCAATACATACAACATCAATTCACAGTTCTTGATTGGTAAAGGTGTGATGGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTTATGCATATTTTCCTGCTGGAAACTGGTTTGATCTCTTCAACTACTCGAGATCAGTGAGTGTAGACTCCGGAGAGTATGTTATGTTGGACGCACCGCCTGATCATATCAATGTACATGTCCGCCAAGGAAACATTTTGGCCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCTCGGAAGACTGCATTCGAACTTTTGGTGGTCATTAGTAGTAGCGGAGAGAGCACCGGAGAAGTGTTCTTGGATGATGCATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTAGTGAAATTCTATTGTGGAGCAGCAAATGGAAGTGTATTTTTGAGCTCAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

790

Amino Acids

88.9

Weight (kDa)

5.74

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 68 - 228 1.2e-09 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 273 - 621 2.1e-118 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 630 - 719 6.2e-25 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 826
AccI GTMKAC 1 cut(s) 2082
AccIII TCCGGA 2 cut(s) 1367, 2087
AciI CCGC 8 cut(s) 358, 361, 552, 738, 1774, 2114, 2140, 2235
AclWI GGATC 5 cut(s) 289, 980, 1337, 1711, 1724
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 5 cut(s) 82, 207, 1630, 2011, 2322
AcuI CTGAAG 2 cut(s) 1716, 1947
AcyI GRCGYC 2 cut(s) 387, 773
AdeI CACNNNGTG 1 cut(s) 1214
AfaI GTAC 6 cut(s) 440, 485, 797, 1203, 1559, 2133
AfiI CCNNNNNNNGG 6 cut(s) 238, 932, 1160, 1658, 1726, 1902
AflIII ACRYGT 2 cut(s) 1165, 2134
AgeI ACCGGT 1 cut(s) 1839
AgsI TTSAA 9 cut(s) 19, 430, 517, 679, 932, 1250, 1630, 1687, 2059
AhdI GACNNNNNGTC 1 cut(s) 619
AjiI CACGTC 1 cut(s) 616
AjnI CCWGG 5 cut(s) 1072, 1158, 1174, 1485, 1618
AjuI GAANNNNNNNTTGG 4 cut(s) 36, 68, 497, 529
Alw21I GWGCWC 4 cut(s) 501, 1785, 2246, 2364
Alw26I GTCTC 2 cut(s) 1507, 1572
AlwI GGATC 5 cut(s) 289, 980, 1337, 1711, 1724
AlwNI CAGNNNCTG 1 cut(s) 1805
Ama87I CYCGRG 2 cut(s) 223, 2065
Aor13HI TCCGGA 2 cut(s) 1367, 2087
AoxI GGCC 9 cut(s) 121, 487, 811, 1172, 1728, 1828, 1904, 2157, 2172
ApeKI GCWGC 5 cut(s) 1586, 1805, 1999, 2189, 2337
ApoI RAATTY 5 cut(s) 82, 207, 1630, 2011, 2322
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AseI ATTAAT 1 cut(s) 1389
AsiGI ACCGGT 1 cut(s) 1839
AspS9I GGNCC 9 cut(s) 149, 433, 549, 594, 767, 1069, 1728, 1890, 1905
AsuC2I CCSGG 1 cut(s) 464
AsuII TTCGAA 2 cut(s) 211, 2208
AsuNHI GCTAGC 1 cut(s) 917
AvaI CYCGRG 2 cut(s) 223, 2065
AvaII GGWCC 7 cut(s) 149, 433, 549, 594, 767, 1069, 1890
BamHI GGATCC 1 cut(s) 1716
BanII GRGCYC 2 cut(s) 1785, 2364
BbsI GAAGAC 3 cut(s) 107, 418, 2204
Bbv12I GWGCWC 4 cut(s) 501, 1785, 2246, 2364
BbvI GCAGC 5 cut(s) 1598, 1792, 2011, 2201, 2349
BceAI ACGGC 2 cut(s) 126, 502
BciT130I CCWGG 5 cut(s) 1074, 1160, 1176, 1487, 1620
BclI TGATCA 2 cut(s) 126, 2119
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 2 cut(s) 1507, 1572
BfaI CTAG 6 cut(s) 918, 1259, 1337, 1530, 1724, 1826
BfmI CTRYAG 2 cut(s) 648, 1705
BfuAI ACCTGC 1 cut(s) 826
BisI GCNGC 6 cut(s) 361, 1587, 1806, 2000, 2190, 2338
BlsI GCNGC 6 cut(s) 362, 1588, 1807, 2001, 2191, 2339
Bme1390I CCNGG 6 cut(s) 464, 1074, 1160, 1176, 1487, 1620
Bme18I GGWCC 7 cut(s) 149, 433, 549, 594, 767, 1069, 1890
BmeRI GACNNNNNGTC 1 cut(s) 619
BmeT110I CYCGRG 2 cut(s) 223, 2065
BmgBI CACGTC 1 cut(s) 616
BmgT120I GGNCC 9 cut(s) 149, 433, 549, 594, 767, 1069, 1728, 1890, 1905
BmrFI CCNGG 6 cut(s) 464, 1074, 1160, 1176, 1487, 1620
BmsI GCATC 8 cut(s) 23, 816, 930, 952, 1002, 1415, 1817, 2257
BmtI GCTAGC 1 cut(s) 921
BpiI GAAGAC 3 cut(s) 107, 418, 2204
BpmI CTGGAG 2 cut(s) 45, 1596
Bpu14I TTCGAA 2 cut(s) 211, 2208
BpuEI CTTGAG 1 cut(s) 908
BpuMI CCSGG 1 cut(s) 464
BsaHI GRCGYC 2 cut(s) 387, 773
BsaI GGTCTC 1 cut(s) 1507
BsaJI CCNNGG 5 cut(s) 490, 1072, 1159, 1486, 2143
BsaWI WCCGGW 5 cut(s) 629, 1367, 1839, 2087, 2246
BsaXI ACNNNNNCTCC 8 cut(s) 398, 428, 1300, 1330, 2082, 2112, 2266, 2296
Bsc4I CCNNNNNNNGG 6 cut(s) 238, 932, 1160, 1658, 1726, 1902
Bse118I RCCGGY 1 cut(s) 1839
Bse1I ACTGG 7 cut(s) 50, 436, 839, 1510, 1574, 1579, 2048
Bse3DI GCAATG 1 cut(s) 1896
BseAI TCCGGA 2 cut(s) 1367, 2087
BseBI CCWGG 5 cut(s) 1074, 1160, 1176, 1487, 1620
BseDI CCNNGG 5 cut(s) 490, 1072, 1159, 1486, 2143
BseLI CCNNNNNNNGG 6 cut(s) 238, 932, 1160, 1658, 1726, 1902
BseMI GCAATG 1 cut(s) 1896
BseNI ACTGG 7 cut(s) 50, 436, 839, 1510, 1574, 1579, 2048
BseRI GAGGAG 4 cut(s) 127, 445, 905, 2308
BseXI GCAGC 5 cut(s) 1598, 1792, 2011, 2201, 2349
BshFI GGCC 9 cut(s) 123, 489, 813, 1174, 1730, 1830, 1906, 2159, 2174
BshTI ACCGGT 1 cut(s) 1839
BsiHKAI GWGCWC 4 cut(s) 501, 1785, 2246, 2364
BsiHKCI CYCGRG 2 cut(s) 223, 2065
BsiSI CCGG 8 cut(s) 464, 630, 776, 1368, 1403, 1840, 2088, 2247
BslFI GGGAC 3 cut(s) 580, 1176, 1903
BslI CCNNNNNNNGG 6 cut(s) 238, 932, 1160, 1658, 1726, 1902
BsmAI GTCTC 2 cut(s) 1507, 1572
BsmFI GGGAC 3 cut(s) 580, 1176, 1903
BsmI GAATGC 1 cut(s) 2204
BsnI GGCC 9 cut(s) 123, 489, 813, 1174, 1730, 1830, 1906, 2159, 2174
Bso31I GGTCTC 1 cut(s) 1507
BsoBI CYCGRG 2 cut(s) 223, 2065
Bsp119I TTCGAA 2 cut(s) 211, 2208
Bsp1286I GDGCHC 4 cut(s) 501, 1785, 2246, 2364
Bsp13I TCCGGA 2 cut(s) 1367, 2087
Bsp1407I TGTACA 2 cut(s) 1201, 2131
BspACI CCGC 8 cut(s) 358, 361, 552, 738, 1774, 2114, 2140, 2235
BspANI GGCC 9 cut(s) 123, 489, 813, 1174, 1730, 1830, 1906, 2159, 2174
BspEI TCCGGA 2 cut(s) 1367, 2087
BspMAI CTGCAG 2 cut(s) 652, 1709
BspMI ACCTGC 1 cut(s) 826
BspOI GCTAGC 1 cut(s) 921
BspPI GGATC 5 cut(s) 289, 980, 1337, 1711, 1724
BspT104I TTCGAA 2 cut(s) 211, 2208
BspTNI GGTCTC 1 cut(s) 1507
BsrDI GCAATG 1 cut(s) 1896
BsrFI RCCGGY 1 cut(s) 1839
BsrGI TGTACA 2 cut(s) 1201, 2131
BsrI ACTGG 7 cut(s) 50, 436, 839, 1510, 1574, 1579, 2048
BssAI RCCGGY 1 cut(s) 1839
BssECI CCNNGG 5 cut(s) 490, 1072, 1159, 1486, 2143
BssNI GRCGYC 2 cut(s) 387, 773
BssT1I CCWWGG 1 cut(s) 2143
Bst2UI CCWGG 5 cut(s) 1074, 1160, 1176, 1487, 1620
Bst4CI ACNGT 5 cut(s) 352, 627, 710, 1399, 1959
Bst6I CTCTTC 2 cut(s) 1646, 2060
BstACI GRCGYC 2 cut(s) 387, 773
BstAUI TGTACA 2 cut(s) 1201, 2131
BstBI TTCGAA 2 cut(s) 211, 2208
BstC8I GCNNGC 3 cut(s) 181, 919, 1709
BstDEI CTNAG 2 cut(s) 203, 1603
BstEII GGTNACC 2 cut(s) 352, 621
BstENI CCTNNNNNAGG 1 cut(s) 930
BstMAI GTCTC 2 cut(s) 1507, 1572
BstMWI GCNNNNNNNGC 3 cut(s) 908, 1811, 2241
BstNI CCWGG 5 cut(s) 1074, 1160, 1176, 1487, 1620
BstNSI RCATGY 2 cut(s) 1169, 2138
BstPI GGTNACC 2 cut(s) 352, 621
BstSCI CCNGG 6 cut(s) 462, 1072, 1158, 1174, 1485, 1618
BstSFI CTRYAG 2 cut(s) 648, 1705
BstV1I GCAGC 5 cut(s) 1598, 1792, 2011, 2201, 2349
BstV2I GAAGAC 3 cut(s) 107, 418, 2204
BstX2I RGATCY 1 cut(s) 1716
BstYI RGATCY 1 cut(s) 1716
BsuRI GGCC 9 cut(s) 123, 489, 813, 1174, 1730, 1830, 1906, 2159, 2174
BtgZI GCGATG 1 cut(s) 1726
BtrI CACGTC 1 cut(s) 616
BtsIMutI CAGTG 3 cut(s) 1503, 1567, 2079
BveI ACCTGC 1 cut(s) 826
Cac8I GCNNGC 3 cut(s) 181, 919, 1709
CaiI CAGNNNCTG 1 cut(s) 1805
Cfr10I RCCGGY 1 cut(s) 1839
Cfr13I GGNCC 9 cut(s) 149, 433, 549, 594, 767, 1069, 1728, 1890, 1905
CseI GACGC 4 cut(s) 395, 781, 1367, 2117
Csp6I GTAC 6 cut(s) 439, 484, 796, 1202, 1558, 2132
CspAI ACCGGT 1 cut(s) 1839
CviAII CATG 8 cut(s) 610, 662, 691, 958, 1166, 1296, 2135, 2271
CviQI GTAC 6 cut(s) 439, 484, 796, 1202, 1558, 2132
DdeI CTNAG 2 cut(s) 203, 1603
DraIII CACNNNGTG 1 cut(s) 1214
DriI GACNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 2 cut(s) 1646, 2060
Eam1105I GACNNNNNGTC 1 cut(s) 619
EarI CTCTTC 2 cut(s) 1646, 2060
EciI GGCGGA 3 cut(s) 753, 1763, 2129
Ecl136II GAGCTC 2 cut(s) 1783, 2362
Eco130I CCWWGG 1 cut(s) 2143
Eco147I AGGCCT 2 cut(s) 1830, 2174
Eco24I GRGCYC 2 cut(s) 1785, 2364
Eco31I GGTCTC 1 cut(s) 1507
Eco47I GGWCC 7 cut(s) 149, 433, 549, 594, 767, 1069, 1890
Eco53kI GAGCTC 2 cut(s) 1783, 2362
Eco57I CTGAAG 2 cut(s) 1716, 1947
Eco88I CYCGRG 2 cut(s) 223, 2065
Eco91I GGTNACC 2 cut(s) 352, 621
EcoICRI GAGCTC 2 cut(s) 1783, 2362
EcoNI CCTNNNNNAGG 1 cut(s) 930
EcoO109I RGGNCCY 4 cut(s) 594, 1728, 1890, 1905
EcoO65I GGTNACC 2 cut(s) 352, 621
EcoRI GAATTC 2 cut(s) 82, 207
EcoRII CCWGG 5 cut(s) 1072, 1158, 1174, 1485, 1618
EcoT14I CCWWGG 1 cut(s) 2143
EcoT22I ATGCAT 2 cut(s) 2026, 2272
EcoT38I GRGCYC 2 cut(s) 1785, 2364
ErhI CCWWGG 1 cut(s) 2143
FaeI CATG 8 cut(s) 613, 665, 694, 961, 1169, 1299, 2138, 2274
FaqI GGGAC 3 cut(s) 580, 1176, 1903
FatI CATG 8 cut(s) 609, 661, 690, 957, 1165, 1295, 2134, 2270
FauI CCCGC 1 cut(s) 365
FbaI TGATCA 2 cut(s) 126, 2119
FblI GTMKAC 1 cut(s) 2082
Fnu4HI GCNGC 6 cut(s) 361, 1587, 1806, 2000, 2190, 2338
FriOI GRGCYC 2 cut(s) 1785, 2364
Fsp4HI GCNGC 6 cut(s) 361, 1587, 1806, 2000, 2190, 2338
FspBI CTAG 6 cut(s) 918, 1259, 1337, 1530, 1724, 1826
GluI GCNGC 6 cut(s) 361, 1587, 1806, 2000, 2190, 2338
GsuI CTGGAG 2 cut(s) 45, 1596
HaeIII GGCC 9 cut(s) 123, 489, 813, 1174, 1730, 1830, 1906, 2159, 2174
HapII CCGG 8 cut(s) 464, 630, 776, 1368, 1403, 1840, 2088, 2247
HgaI GACGC 4 cut(s) 395, 781, 1367, 2117
Hin1I GRCGYC 2 cut(s) 387, 773
Hin1II CATG 8 cut(s) 613, 665, 694, 961, 1169, 1299, 2138, 2274
HincII GTYRAC 2 cut(s) 748, 1186
HindII GTYRAC 2 cut(s) 748, 1186
HindIII AAGCTT 1 cut(s) 170
HpaI GTTAAC 1 cut(s) 1186
HpaII CCGG 8 cut(s) 464, 630, 776, 1368, 1403, 1840, 2088, 2247
Hpy166II GTNNAC 7 cut(s) 748, 798, 1023, 1186, 1217, 1560, 2083
Hpy8I GTNNAC 7 cut(s) 748, 798, 1023, 1186, 1217, 1560, 2083
Hpy99I CGWCG 3 cut(s) 389, 710, 775
HpyAV CCTTC 7 cut(s) 266, 420, 523, 926, 1043, 1111, 1813
HpyCH4III ACNGT 5 cut(s) 352, 627, 710, 1399, 1959
HpyCH4IV ACGT 2 cut(s) 615, 1141
HpyF10VI GCNNNNNNNGC 3 cut(s) 908, 1811, 2241
HpyF3I CTNAG 2 cut(s) 203, 1603
HpySE526I ACGT 2 cut(s) 615, 1141
Hsp92I GRCGYC 2 cut(s) 387, 773
Hsp92II CATG 8 cut(s) 613, 665, 694, 961, 1169, 1299, 2138, 2274
KflI GGGWCCC 2 cut(s) 594, 1890
Kpn2I TCCGGA 2 cut(s) 1367, 2087
Ksp22I TGATCA 2 cut(s) 126, 2119
KspAI GTTAAC 1 cut(s) 1186
Lsp1109I GCAGC 5 cut(s) 1598, 1792, 2011, 2201, 2349
LweI GCATC 8 cut(s) 23, 816, 930, 952, 1002, 1415, 1817, 2257
MaeI CTAG 6 cut(s) 918, 1259, 1337, 1530, 1724, 1826
MaeII ACGT 2 cut(s) 615, 1141
MflI RGATCY 1 cut(s) 1716
MhlI GDGCHC 4 cut(s) 501, 1785, 2246, 2364
MlyI GAGTC 6 cut(s) 215, 265, 529, 1380, 1632, 2078
MmeI TCCRAC 5 cut(s) 429, 794, 1406, 1639, 2085
Mph1103I ATGCAT 2 cut(s) 2026, 2272
MroI TCCGGA 2 cut(s) 1367, 2087
MseI TTAA 4 cut(s) 1004, 1185, 1389, 1493
MslI CAYNNNNRTG 4 cut(s) 614, 1041, 1865, 2127
MspI CCGG 8 cut(s) 464, 630, 776, 1368, 1403, 1840, 2088, 2247
MspR9I CCNGG 6 cut(s) 464, 1074, 1160, 1176, 1487, 1620
Mva1269I GAATGC 1 cut(s) 2204
MvaI CCWGG 5 cut(s) 1074, 1160, 1176, 1487, 1620
MwoI GCNNNNNNNGC 3 cut(s) 908, 1811, 2241
NciI CCSGG 1 cut(s) 464
NheI GCTAGC 1 cut(s) 917
NlaIII CATG 8 cut(s) 613, 665, 694, 961, 1169, 1299, 2138, 2274
NmuCI GTSAC 6 cut(s) 352, 621, 710, 1501, 1598, 1986
NsiI ATGCAT 2 cut(s) 2026, 2272
NspI RCATGY 2 cut(s) 1169, 2138
NspV TTCGAA 2 cut(s) 211, 2208
PaeR7I CTCGAG 2 cut(s) 223, 2065
PceI AGGCCT 2 cut(s) 1830, 2174
PciI ACATGT 2 cut(s) 1165, 2134
PctI GAATGC 1 cut(s) 2204
PfeI GAWTC 6 cut(s) 89, 846, 1364, 1469, 1677, 1796
PfoI TCCNGGA 1 cut(s) 462
PinAI ACCGGT 1 cut(s) 1839
PkrI GCNGC 6 cut(s) 362, 1588, 1807, 2001, 2191, 2339
PleI GAGTC 6 cut(s) 215, 265, 529, 1379, 1632, 2078
PpsI GAGTC 6 cut(s) 215, 265, 529, 1379, 1632, 2078
PpuMI RGGWCCY 2 cut(s) 594, 1890
PscI ACATGT 2 cut(s) 1165, 2134
PshBI ATTAAT 1 cut(s) 1389
Psp124BI GAGCTC 2 cut(s) 1785, 2364
Psp5II RGGWCCY 2 cut(s) 594, 1890
Psp6I CCWGG 5 cut(s) 1072, 1158, 1174, 1485, 1618
PspEI GGTNACC 2 cut(s) 352, 621
PspGI CCWGG 5 cut(s) 1072, 1158, 1174, 1485, 1618
PspPI GGNCC 9 cut(s) 149, 433, 549, 594, 767, 1069, 1728, 1890, 1905
PspPPI RGGWCCY 2 cut(s) 594, 1890
PspXI VCTCGAGB 1 cut(s) 223
PsrI GAACNNNNNNTAC 2 cut(s) 788, 820
PstI CTGCAG 2 cut(s) 652, 1709
PstNI CAGNNNCTG 1 cut(s) 1805
PsuI RGATCY 1 cut(s) 1716
RsaI GTAC 6 cut(s) 440, 485, 797, 1203, 1559, 2133
RsaNI GTAC 6 cut(s) 439, 484, 796, 1202, 1558, 2132
RseI CAYNNNNRTG 4 cut(s) 614, 1041, 1865, 2127
SacI GAGCTC 2 cut(s) 1785, 2364
SaqAI TTAA 4 cut(s) 1004, 1185, 1389, 1493
SatI GCNGC 6 cut(s) 361, 1587, 1806, 2000, 2190, 2338
Sau96I GGNCC 9 cut(s) 149, 433, 549, 594, 767, 1069, 1728, 1890, 1905
SchI GAGTC 6 cut(s) 215, 265, 529, 1380, 1632, 2078
ScrFI CCNGG 6 cut(s) 464, 1074, 1160, 1176, 1487, 1620
SduI GDGCHC 4 cut(s) 501, 1785, 2246, 2364
SfaNI GCATC 8 cut(s) 23, 816, 930, 952, 1002, 1415, 1817, 2257
SfcI CTRYAG 2 cut(s) 648, 1705
Sfr274I CTCGAG 2 cut(s) 223, 2065
SfuI TTCGAA 2 cut(s) 211, 2208
SinI GGWCC 7 cut(s) 149, 433, 549, 594, 767, 1069, 1890
SlaI CTCGAG 2 cut(s) 223, 2065
SmiMI CAYNNNNRTG 4 cut(s) 614, 1041, 1865, 2127
SmlI CTYRAG 3 cut(s) 223, 887, 2065
SmoI CTYRAG 3 cut(s) 223, 887, 2065
SseBI AGGCCT 2 cut(s) 1830, 2174
SsiI CCGC 8 cut(s) 358, 361, 552, 738, 1774, 2114, 2140, 2235
SspMI CTAG 6 cut(s) 918, 1259, 1337, 1530, 1724, 1826
SstI GAGCTC 2 cut(s) 1785, 2364
StuI AGGCCT 2 cut(s) 1830, 2174
StyD4I CCNGG 6 cut(s) 462, 1072, 1158, 1174, 1485, 1618
StyI CCWWGG 1 cut(s) 2143
TaaI ACNGT 5 cut(s) 352, 627, 710, 1399, 1959
TaiI ACGT 2 cut(s) 618, 1144
TaqI TCGA 9 cut(s) 211, 224, 265, 293, 384, 472, 2066, 2208, 2284
TatI WGTACW 5 cut(s) 438, 795, 1201, 1557, 2131
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 6 cut(s) 89, 846, 1364, 1469, 1677, 1796
Tru1I TTAA 4 cut(s) 1004, 1185, 1389, 1493
Tru9I TTAA 4 cut(s) 1004, 1185, 1389, 1493
TscAI CASTG 3 cut(s) 1510, 1574, 2079
TseFI GTSAC 6 cut(s) 352, 621, 710, 1501, 1598, 1986
TseI GCWGC 5 cut(s) 1586, 1805, 1999, 2189, 2337
Tsp45I GTSAC 6 cut(s) 352, 621, 710, 1501, 1598, 1986
TspDTI ATGAA 5 cut(s) 17, 201, 796, 1301, 1436
TspGWI ACGGA 2 cut(s) 815, 1823
TspRI CASTG 3 cut(s) 1510, 1574, 2079
VpaK11BI GGWCC 7 cut(s) 149, 433, 549, 594, 767, 1069, 1890
VspI ATTAAT 1 cut(s) 1389
XagI CCTNNNNNAGG 1 cut(s) 930
XapI RAATTY 5 cut(s) 82, 207, 1630, 2011, 2322
XceI RCATGY 2 cut(s) 1169, 2138
XhoI CTCGAG 2 cut(s) 223, 2065
XmiI GTMKAC 1 cut(s) 2082
XspI CTAG 6 cut(s) 918, 1259, 1337, 1530, 1724, 1826
Zsp2I ATGCAT 2 cut(s) 2026, 2272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.