Belongs to the glycosyl hydrolase 31 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 188 |
| AclWI | GGATC | 1 cut(s) | 140 |
| AcsI | RAATTY | 1 cut(s) | 110 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 156, 160 |
| AgsI | TTSAA | 1 cut(s) | 220 |
| AluBI | AGCT | 1 cut(s) | 90 |
| AluI | AGCT | 1 cut(s) | 90 |
| Alw21I | GWGCWC | 1 cut(s) | 92 |
| AlwI | GGATC | 1 cut(s) | 140 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 340 |
| AoxI | GGCC | 1 cut(s) | 240 |
| ApoI | RAATTY | 1 cut(s) | 110 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 61, 93 |
| BanII | GRGCYC | 1 cut(s) | 92 |
| Bbv12I | GWGCWC | 1 cut(s) | 92 |
| BccI | CCATC | 1 cut(s) | 176 |
| BciVI | GTATCC | 1 cut(s) | 298 |
| BfaI | CTAG | 3 cut(s) | 62, 80, 355 |
| BfmI | CTRYAG | 1 cut(s) | 297 |
| BfuI | GTATCC | 1 cut(s) | 298 |
| BsaJI | CCNNGG | 2 cut(s) | 124, 154 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 299, 329 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 156, 160 |
| Bse1I | ACTGG | 2 cut(s) | 282, 345 |
| BseDI | CCNNGG | 2 cut(s) | 124, 154 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 156, 160 |
| BseNI | ACTGG | 2 cut(s) | 282, 345 |
| BseRI | GAGGAG | 1 cut(s) | 33 |
| BsgI | GTGCAG | 1 cut(s) | 42 |
| BshFI | GGCC | 1 cut(s) | 242 |
| BsiHKAI | GWGCWC | 1 cut(s) | 92 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 156, 160 |
| BsnI | GGCC | 1 cut(s) | 242 |
| Bsp1286I | GDGCHC | 1 cut(s) | 92 |
| Bsp143I | GATC | 1 cut(s) | 132 |
| BspANI | GGCC | 1 cut(s) | 242 |
| BspPI | GGATC | 1 cut(s) | 140 |
| BsrI | ACTGG | 2 cut(s) | 282, 345 |
| BssECI | CCNNGG | 2 cut(s) | 124, 154 |
| BssMI | GATC | 1 cut(s) | 132 |
| BssNAI | GTATAC | 1 cut(s) | 189 |
| BssT1I | CCWWGG | 2 cut(s) | 124, 154 |
| Bst1107I | GTATAC | 1 cut(s) | 189 |
| Bst4CI | ACNGT | 2 cut(s) | 151, 254 |
| BstKTI | GATC | 1 cut(s) | 135 |
| BstMBI | GATC | 1 cut(s) | 132 |
| BstSFI | CTRYAG | 1 cut(s) | 297 |
| BstZ17I | GTATAC | 1 cut(s) | 189 |
| BsuI | GTATCC | 1 cut(s) | 298 |
| BsuRI | GGCC | 1 cut(s) | 242 |
| BtsIMutI | CAGTG | 1 cut(s) | 289 |
| CaiI | CAGNNNCTG | 1 cut(s) | 340 |
| CviJI | RGCY | 4 cut(s) | 90, 123, 242, 328 |
| CviKI_1 | RGCY | 4 cut(s) | 90, 123, 242, 328 |
| DpnI | GATC | 1 cut(s) | 134 |
| DpnII | GATC | 1 cut(s) | 132 |
| Ecl136II | GAGCTC | 1 cut(s) | 90 |
| Eco130I | CCWWGG | 2 cut(s) | 124, 154 |
| Eco24I | GRGCYC | 1 cut(s) | 92 |
| Eco53kI | GAGCTC | 1 cut(s) | 90 |
| EcoICRI | GAGCTC | 1 cut(s) | 90 |
| EcoT14I | CCWWGG | 2 cut(s) | 124, 154 |
| EcoT38I | GRGCYC | 1 cut(s) | 92 |
| ErhI | CCWWGG | 2 cut(s) | 124, 154 |
| FaiI | YATR | 3 cut(s) | 189, 258, 299 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 230, 262 |
| FblI | GTMKAC | 1 cut(s) | 188 |
| FriOI | GRGCYC | 1 cut(s) | 92 |
| FspBI | CTAG | 3 cut(s) | 62, 80, 355 |
| HaeIII | GGCC | 1 cut(s) | 242 |
| HinfI | GANTC | 2 cut(s) | 278, 331 |
| Hpy166II | GTNNAC | 1 cut(s) | 189 |
| Hpy188I | TCNGA | 1 cut(s) | 336 |
| Hpy188III | TCNNGA | 1 cut(s) | 43 |
| Hpy8I | GTNNAC | 1 cut(s) | 189 |
| HpyAV | CCTTC | 1 cut(s) | 302 |
| HpyCH4III | ACNGT | 2 cut(s) | 151, 254 |
| HpyCH4V | TGCA | 3 cut(s) | 59, 116, 176 |
| Kzo9I | GATC | 1 cut(s) | 132 |
| LmnI | GCTCC | 1 cut(s) | 209 |
| LpnPI | CCDG | 2 cut(s) | 295, 326 |
| MaeI | CTAG | 3 cut(s) | 62, 80, 355 |
| MalI | GATC | 1 cut(s) | 134 |
| MboI | GATC | 1 cut(s) | 132 |
| MhlI | GDGCHC | 1 cut(s) | 92 |
| MluCI | AATT | 3 cut(s) | 110, 144, 198 |
| MlyI | GAGTC | 1 cut(s) | 287 |
| MmeI | TCCRAC | 3 cut(s) | 110, 141, 297 |
| MnlI | CCTC | 4 cut(s) | 11, 16, 20, 39 |
| MseI | TTAA | 3 cut(s) | 119, 171, 237 |
| MslI | CAYNNNNRTG | 1 cut(s) | 255 |
| NdeII | GATC | 1 cut(s) | 132 |
| PfeI | GAWTC | 1 cut(s) | 331 |
| PleI | GAGTC | 1 cut(s) | 286 |
| PpsI | GAGTC | 1 cut(s) | 286 |
| Psp124BI | GAGCTC | 1 cut(s) | 92 |
| PstNI | CAGNNNCTG | 1 cut(s) | 340 |
| RseI | CAYNNNNRTG | 1 cut(s) | 255 |
| SacI | GAGCTC | 1 cut(s) | 92 |
| SaqAI | TTAA | 3 cut(s) | 119, 171, 237 |
| Sau3AI | GATC | 1 cut(s) | 132 |
| SchI | GAGTC | 1 cut(s) | 287 |
| SduI | GDGCHC | 1 cut(s) | 92 |
| SetI | ASST | 5 cut(s) | 50, 92, 99, 130, 276 |
| SfcI | CTRYAG | 1 cut(s) | 297 |
| SgeI | CNNG | 8 cut(s) | 55, 74, 92, 137, 167, 283, 294, 353 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 255 |
| Sse9I | AATT | 3 cut(s) | 110, 144, 198 |
| SspMI | CTAG | 3 cut(s) | 62, 80, 355 |
| SstI | GAGCTC | 1 cut(s) | 92 |
| StyI | CCWWGG | 2 cut(s) | 124, 154 |
| TaaI | ACNGT | 2 cut(s) | 151, 254 |
| TaqI | TCGA | 2 cut(s) | 9, 99 |
| TasI | AATT | 3 cut(s) | 110, 144, 198 |
| TfiI | GAWTC | 1 cut(s) | 331 |
| Tru1I | TTAA | 3 cut(s) | 119, 171, 237 |
| Tru9I | TTAA | 3 cut(s) | 119, 171, 237 |
| TscAI | CASTG | 1 cut(s) | 289 |
| TspDTI | ATGAA | 1 cut(s) | 338 |
| TspRI | CASTG | 1 cut(s) | 289 |
| XapI | RAATTY | 1 cut(s) | 110 |
| XmiI | GTMKAC | 1 cut(s) | 188 |
| XspI | CTAG | 3 cut(s) | 62, 80, 355 |