Rh5AG136700

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000882
Physical Location & Seq
Forward (+)
14007805 .. 14026201
18397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG136700.1

Sequence Viewer

Length: 2721 bp
ATGGGGAGGTTGGTGGGTGTGCTGGGGTTGCAACAGGGGGTTTGCAGGGGAGGGGCAAAGGATGATCAAGGGATGTGTCGGGATGTGCAGGTGGTGGGGGGCATGGTTGTGGAGCTACATTGTTTAGTTGCAGAAGAACCAATTGGGTATGGATATAAAGTTGAGTCTGTGAATTCTGATTCCTCTGGGAAGACATTGACGGCTAATCTTGGCCTGATCAATAGCTCCTCAATCTATGGACCTGACATCCCAAATCTAAAGCTTTATGCCAGCTATGAAACCAAAGAAAGACTAAGAATTCGAATAACAGACTCGAGTAACCAAAGATGGGAAATCCCACAAGACCTTCTCCCTCGACAAACTCCACAAACATCCCACCATTCGATCCCCGAAAACGATCTCATTTTCACCCTCCACACCACCACCCCGTTTGGCTTCACGATCACCCGCCGCTTCTCCAATGATGTCGTATTCGACGCCACCCCAAACCCCTCCAACCCTTCCTCCATCCTCGTCTTCAAGGACCAGTACATCCAGCTTTCCTCCTCTCTCCCGGAAACCCGATCTTCCCTGTACGGCCTCGGTGAGCACACCAAAGCCTCCTTCAAGCTCCAATCCAATCAGACTCTAACACTCTGGACTGCCGACATTTTCAGCTCCATCCCCGACCTCAATCTCTATGGGTCCCACCCATTTTACATGGACGTGAGGTCACCGTCCGGTGATGAGAAAGTTACTGCAGGAACCACTCATGGAGTTTTGCTATTGAATAGTAATGGCATGGATGTAAATTACGACGGCGACAGGATTACATATAAAGTGATTGGCGGAGTTGTTGACTTGTATTTTTTTTCTGGGCCGACGCCGGAGTTGGTGATGGAGCAATACACGGAGTTCATTGGCCGACCTGCCCCGATGCCCTACTGGTCTTTTGGATTCCACCAATGTAGATATAGCTACAAGGATGTTGCTGACCTTGAGGAGGTGGTTGCTGGTTATGCAAATGCTAGCATCCCTCTTGAAGGTATTTGGACAGACATTGACTACATGGATGCTTACAAGGATTTCACTCTTGATCCCATCAACTTTCCATTAAGTAAGATGCAGAACTTTACAAACACCCTTCACCAAAATGGCCAGAAATATGTGCCGATCTTGGACCCTGGTATTAGTATAAATGAGAGCTATGGAAGTTACATCAGAGGGAAGGAAGCAGATATATACATAAAACGTGATGGTATTCCTTACCAGGGACATGTATGGCCTGGTGATGTTAACTATCCTGATTTTGTACATCCACAAAGTGAACAATTTTGGGCTAATGAGATCAAACTATTTCAAGACCAACTAGCTTTTGATGGTCTTTGGCTTGATATGAATGAGCCATGCAATTTGATAACATCTGCTCCCACTCCATATTCTACACTAGATGATCCACCTTACAAAATCAATGATTCCGGAGTCCAGCGTCCGACCATTAATAAGACTGTTCCGGCATCAGCTCTACACTTTGGTAATATAACAGAGTATAATGTTCATAACCTATATGGGTTCTTGGAATCTAAAGCAACACACCAGGGGTTAATCAATGTCACTGGTAAGAGACCATTTATACTCACTAGATCAACCTTTGTAAGCTCTGGTAAGTACACAGCACACTGGACTGGAGACAATGCAGCAAGATGGAGTGACTTGGCATACACAATTCCAGGCATTTTGAATTTTGGACTCTTCGGAGTTCCGATGGTTGGAGCTGATATATGTGGATTCACTTTGAATACTACTGAAGAACTCTGCAGGCGATGGATCCAGCTAGGGGCCTTTTATCCTTTTGCAAGAGATCATTCAGATATATTTACAATCCGCCAAGAGCTCTATGTTTGGGATTCAGTTGCTGCATCAGCAAGGAAGGTGCTAGGCCTCCGTTACCGGTTGCTCCCCTTGTTCTACACATCAATGTATCAGGCACACAAAAAAGGGACCCCCATTGCAAGGCCTCTTTTCTTCTCGTTTCCTGAAGATACCAATACATACAACATCAATTCACAGTTCTTGATTGGTAAAGGTGTGATGGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTTATGCATATGTTCCTGCTGGAAACTGGTTTGATCTCTTCAACTACTCGAGATCAGTGAGTGTAGACTCCGGAGAGTATGTTATGTTGGACGCACCGCCTGATCATATCAATGTACATGTCCGCGAAGGAAACATTTTGGCCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCTCGGAAGACTGCATTCGAACTTTTGGTGGTCATTAGTAGTAGCGGAGATAGCACTGGAGAAGTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTAGTGAAATTCTATTGTGGAGCAGCAAACGGAAGTGTACTTTTGAGCTCAAAAGTTGAAAATGGAGGATTTGCTTTGAGCCAAAAATGGATCATTGATAAAGTTACCTTCATTGGGTTGGAAAAAGTTGATGGATTAGAAGGGTTTGCAGTCAACTTTATTAAGGGAACAAATTTGAAAGGAATGTGGGATGTCAATGCCAACTTTCACGGTGATAAGCGTTTTGTCATGGTAAAAATATCCAGTGTATCCATTCCTATAGGAAAAGAGTTTGAGTTGGAGCTGAGCCTGAAGCGTGACACCGGTGAAAACACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

906

Amino Acids

100.83

Weight (kDa)

5.22

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 98 - 258 1.6e-09 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 303 - 651 4.1e-118 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 660 - 749 2.2e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 79
Acc36I ACCTGC 2 cut(s) 79, 916
AccI GTMKAC 1 cut(s) 2172
AccII CGCG 1 cut(s) 2232
AccIII TCCGGA 2 cut(s) 1457, 2177
AciI CCGC 7 cut(s) 448, 451, 828, 1864, 2204, 2230, 2325
AclWI GGATC 6 cut(s) 379, 1070, 1427, 1801, 1814, 2498
AcoI YGGCCR 2 cut(s) 901, 1135
AcsI RAATTY 6 cut(s) 172, 297, 1720, 2101, 2408, 2572
AcuI CTGAAG 3 cut(s) 1806, 2037, 2711
AcyI GRCGYC 2 cut(s) 477, 863
AdeI CACNNNGTG 1 cut(s) 1304
AfaI GTAC 6 cut(s) 530, 575, 1293, 1649, 2223, 2439
AfiI CCNNNNNNNGG 8 cut(s) 328, 982, 1022, 1250, 1748, 1816, 1992, 2514
AflIII ACRYGT 2 cut(s) 1255, 2224
AgeI ACCGGT 2 cut(s) 1929, 2702
AhdI GACNNNNNGTC 1 cut(s) 709
AjiI CACGTC 1 cut(s) 706
AjnI CCWGG 5 cut(s) 1162, 1248, 1264, 1575, 1708
AjuI GAANNNNNNNTTGG 4 cut(s) 126, 158, 587, 619
Alw21I GWGCWC 3 cut(s) 591, 1875, 2450
Alw26I GTCTC 2 cut(s) 1597, 1662
AlwI GGATC 6 cut(s) 379, 1070, 1427, 1801, 1814, 2498
AlwNI CAGNNNCTG 1 cut(s) 1895
Ama87I CYCGRG 2 cut(s) 313, 2155
Aor13HI TCCGGA 2 cut(s) 1457, 2177
ApeKI GCWGC 5 cut(s) 1676, 1895, 2089, 2279, 2423
ApoI RAATTY 6 cut(s) 172, 297, 1720, 2101, 2408, 2572
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AseI ATTAAT 1 cut(s) 1479
AsiGI ACCGGT 2 cut(s) 1929, 2702
AspS9I GGNCC 7 cut(s) 239, 523, 684, 857, 1159, 1818, 1980
AsuC2I CCSGG 1 cut(s) 554
AsuII TTCGAA 2 cut(s) 301, 2298
AsuNHI GCTAGC 1 cut(s) 1007
AvaI CYCGRG 2 cut(s) 313, 2155
AvaII GGWCC 5 cut(s) 239, 523, 684, 1159, 1980
BalI TGGCCA 1 cut(s) 1137
BamHI GGATCC 1 cut(s) 1806
BanII GRGCYC 2 cut(s) 1875, 2450
BbsI GAAGAC 3 cut(s) 197, 508, 2294
Bbv12I GWGCWC 3 cut(s) 591, 1875, 2450
BbvI GCAGC 5 cut(s) 1688, 1882, 2101, 2291, 2435
BceAI ACGGC 3 cut(s) 216, 592, 814
BciT130I CCWGG 5 cut(s) 1164, 1250, 1266, 1577, 1710
BciVI GTATCC 1 cut(s) 2659
BclI TGATCA 3 cut(s) 64, 216, 2209
BcnI CCSGG 1 cut(s) 554
BcoDI GTCTC 2 cut(s) 1597, 1662
BfaI CTAG 6 cut(s) 1008, 1349, 1427, 1620, 1814, 1916
BfmI CTRYAG 3 cut(s) 738, 1795, 2658
BfuAI ACCTGC 2 cut(s) 79, 916
BfuI GTATCC 1 cut(s) 2659
BisI GCNGC 6 cut(s) 451, 1677, 1896, 2090, 2280, 2424
BlpI GCTNAGC 1 cut(s) 2684
BlsI GCNGC 6 cut(s) 452, 1678, 1897, 2091, 2281, 2425
Bme1390I CCNGG 6 cut(s) 554, 1164, 1250, 1266, 1577, 1710
Bme18I GGWCC 5 cut(s) 239, 523, 684, 1159, 1980
BmeRI GACNNNNNGTC 1 cut(s) 709
BmeT110I CYCGRG 2 cut(s) 313, 2155
BmgBI CACGTC 1 cut(s) 706
BmgT120I GGNCC 7 cut(s) 239, 523, 684, 857, 1159, 1818, 1980
BmiI GGNNCC 8 cut(s) 685, 686, 745, 1161, 1808, 1819, 1981, 1982
BmrFI CCNGG 6 cut(s) 554, 1164, 1250, 1266, 1577, 1710
BmsI GCATC 6 cut(s) 906, 1020, 1042, 1092, 1505, 1907
BmtI GCTAGC 1 cut(s) 1011
BpiI GAAGAC 3 cut(s) 197, 508, 2294
BpmI CTGGAG 2 cut(s) 1686, 2358
Bpu1102I GCTNAGC 1 cut(s) 2684
Bpu14I TTCGAA 2 cut(s) 301, 2298
BpuEI CTTGAG 1 cut(s) 998
BpuMI CCSGG 1 cut(s) 554
BsaHI GRCGYC 2 cut(s) 477, 863
BsaI GGTCTC 1 cut(s) 1597
BsaJI CCNNGG 4 cut(s) 580, 1162, 1249, 1576
BsaWI WCCGGW 5 cut(s) 719, 1457, 1929, 2177, 2702
BsaXI ACNNNNNCTCC 8 cut(s) 488, 518, 1390, 1420, 2172, 2202, 2352, 2382
Bsc4I CCNNNNNNNGG 8 cut(s) 328, 982, 1022, 1250, 1748, 1816, 1992, 2514
Bse118I RCCGGY 2 cut(s) 1929, 2702
Bse1I ACTGG 8 cut(s) 526, 929, 1600, 1664, 1669, 2138, 2341, 2643
Bse3DI GCAATG 1 cut(s) 1986
BseAI TCCGGA 2 cut(s) 1457, 2177
BseBI CCWGG 5 cut(s) 1164, 1250, 1266, 1577, 1710
BseDI CCNNGG 4 cut(s) 580, 1162, 1249, 1576
BseLI CCNNNNNNNGG 8 cut(s) 328, 982, 1022, 1250, 1748, 1816, 1992, 2514
BseMI GCAATG 1 cut(s) 1986
BseMII CTCAG 1 cut(s) 2675
BseNI ACTGG 8 cut(s) 526, 929, 1600, 1664, 1669, 2138, 2341, 2643
BseRI GAGGAG 4 cut(s) 217, 535, 995, 2394
BseXI GCAGC 5 cut(s) 1688, 1882, 2101, 2291, 2435
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 2232
BshTI ACCGGT 2 cut(s) 1929, 2702
BsiHKAI GWGCWC 3 cut(s) 591, 1875, 2450
BsiHKCI CYCGRG 2 cut(s) 313, 2155
BsiSI CCGG 8 cut(s) 554, 720, 866, 1458, 1493, 1930, 2178, 2703
BslFI GGGAC 3 cut(s) 670, 1266, 1993
BslI CCNNNNNNNGG 8 cut(s) 328, 982, 1022, 1250, 1748, 1816, 1992, 2514
BsmAI GTCTC 2 cut(s) 1597, 1662
BsmFI GGGAC 3 cut(s) 670, 1266, 1993
BsmI GAATGC 1 cut(s) 2294
Bso31I GGTCTC 1 cut(s) 1597
BsoBI CYCGRG 2 cut(s) 313, 2155
Bsp119I TTCGAA 2 cut(s) 301, 2298
Bsp1286I GDGCHC 3 cut(s) 591, 1875, 2450
Bsp13I TCCGGA 2 cut(s) 1457, 2177
Bsp1407I TGTACA 2 cut(s) 1291, 2221
Bsp1720I GCTNAGC 1 cut(s) 2684
BspACI CCGC 7 cut(s) 448, 451, 828, 1864, 2204, 2230, 2325
BspCNI CTCAG 1 cut(s) 2676
BspEI TCCGGA 2 cut(s) 1457, 2177
BspFNI CGCG 1 cut(s) 2232
BspLI GGNNCC 8 cut(s) 685, 686, 745, 1161, 1808, 1819, 1981, 1982
BspMAI CTGCAG 2 cut(s) 742, 1799
BspMI ACCTGC 2 cut(s) 79, 916
BspOI GCTAGC 1 cut(s) 1011
BspPI GGATC 6 cut(s) 379, 1070, 1427, 1801, 1814, 2498
BspT104I TTCGAA 2 cut(s) 301, 2298
BspTNI GGTCTC 1 cut(s) 1597
BsrDI GCAATG 1 cut(s) 1986
BsrFI RCCGGY 2 cut(s) 1929, 2702
BsrGI TGTACA 2 cut(s) 1291, 2221
BsrI ACTGG 8 cut(s) 526, 929, 1600, 1664, 1669, 2138, 2341, 2643
BssAI RCCGGY 2 cut(s) 1929, 2702
BssECI CCNNGG 4 cut(s) 580, 1162, 1249, 1576
BssNI GRCGYC 2 cut(s) 477, 863
Bst2UI CCWGG 5 cut(s) 1164, 1250, 1266, 1577, 1710
Bst4CI ACNGT 4 cut(s) 717, 1489, 2049, 2612
Bst6I CTCTTC 2 cut(s) 1736, 2150
BstACI GRCGYC 2 cut(s) 477, 863
BstAUI TGTACA 2 cut(s) 1291, 2221
BstBI TTCGAA 2 cut(s) 301, 2298
BstC8I GCNNGC 3 cut(s) 271, 1009, 1799
BstDEI CTNAG 2 cut(s) 293, 2684
BstEII GGTNACC 1 cut(s) 711
BstENI CCTNNNNNAGG 2 cut(s) 980, 1020
BstFNI CGCG 1 cut(s) 2232
BstMAI GTCTC 2 cut(s) 1597, 1662
BstMWI GCNNNNNNNGC 4 cut(s) 28, 998, 1901, 2331
BstNI CCWGG 5 cut(s) 1164, 1250, 1266, 1577, 1710
BstNSI RCATGY 2 cut(s) 1259, 2228
BstPI GGTNACC 1 cut(s) 711
BstSCI CCNGG 6 cut(s) 552, 1162, 1248, 1264, 1575, 1708
BstSFI CTRYAG 3 cut(s) 738, 1795, 2658
BstUI CGCG 1 cut(s) 2232
BstV1I GCAGC 5 cut(s) 1688, 1882, 2101, 2291, 2435
BstV2I GAAGAC 3 cut(s) 197, 508, 2294
BstX2I RGATCY 1 cut(s) 1806
BstYI RGATCY 1 cut(s) 1806
BsuI GTATCC 1 cut(s) 2659
BtgZI GCGATG 1 cut(s) 1816
BtrI CACGTC 1 cut(s) 706
BtsIMutI CAGTG 5 cut(s) 1593, 1657, 2169, 2334, 2650
BveI ACCTGC 2 cut(s) 79, 916
Cac8I GCNNGC 3 cut(s) 271, 1009, 1799
CaiI CAGNNNCTG 1 cut(s) 1895
Cfr10I RCCGGY 2 cut(s) 1929, 2702
Cfr13I GGNCC 7 cut(s) 239, 523, 684, 857, 1159, 1818, 1980
CseI GACGC 4 cut(s) 485, 871, 1457, 2207
Csp6I GTAC 6 cut(s) 529, 574, 1292, 1648, 2222, 2438
CspAI ACCGGT 2 cut(s) 1929, 2702
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 9 cut(s) 103, 700, 752, 781, 1048, 1256, 1386, 2225, 2629
CviQI GTAC 6 cut(s) 529, 574, 1292, 1648, 2222, 2438
DdeI CTNAG 2 cut(s) 293, 2684
DraIII CACNNNGTG 1 cut(s) 1304
DriI GACNNNNNGTC 1 cut(s) 709
EaeI YGGCCR 2 cut(s) 901, 1135
Eam1104I CTCTTC 2 cut(s) 1736, 2150
Eam1105I GACNNNNNGTC 1 cut(s) 709
EarI CTCTTC 2 cut(s) 1736, 2150
EciI GGCGGA 2 cut(s) 843, 1853
Ecl136II GAGCTC 2 cut(s) 1873, 2448
Eco147I AGGCCT 3 cut(s) 1920, 1996, 2264
Eco24I GRGCYC 2 cut(s) 1875, 2450
Eco31I GGTCTC 1 cut(s) 1597
Eco47I GGWCC 5 cut(s) 239, 523, 684, 1159, 1980
Eco53kI GAGCTC 2 cut(s) 1873, 2448
Eco57I CTGAAG 3 cut(s) 1806, 2037, 2711
Eco88I CYCGRG 2 cut(s) 313, 2155
Eco91I GGTNACC 1 cut(s) 711
EcoICRI GAGCTC 2 cut(s) 1873, 2448
EcoNI CCTNNNNNAGG 2 cut(s) 980, 1020
EcoO109I RGGNCCY 3 cut(s) 684, 1818, 1980
EcoO65I GGTNACC 1 cut(s) 711
EcoRI GAATTC 2 cut(s) 172, 297
EcoRII CCWGG 5 cut(s) 1162, 1248, 1264, 1575, 1708
EcoT22I ATGCAT 1 cut(s) 2116
EcoT38I GRGCYC 2 cut(s) 1875, 2450
FaeI CATG 9 cut(s) 106, 703, 755, 784, 1051, 1259, 1389, 2228, 2632
FaqI GGGAC 3 cut(s) 670, 1266, 1993
FatI CATG 9 cut(s) 102, 699, 751, 780, 1047, 1255, 1385, 2224, 2628
FauI CCCGC 1 cut(s) 455
FauNDI CATATG 1 cut(s) 2116
FbaI TGATCA 3 cut(s) 64, 216, 2209
FblI GTMKAC 1 cut(s) 2172
Fnu4HI GCNGC 6 cut(s) 451, 1677, 1896, 2090, 2280, 2424
FriOI GRGCYC 2 cut(s) 1875, 2450
Fsp4HI GCNGC 6 cut(s) 451, 1677, 1896, 2090, 2280, 2424
FspBI CTAG 6 cut(s) 1008, 1349, 1427, 1620, 1814, 1916
GluI GCNGC 6 cut(s) 451, 1677, 1896, 2090, 2280, 2424
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 2 cut(s) 1686, 2358
HapII CCGG 8 cut(s) 554, 720, 866, 1458, 1493, 1930, 2178, 2703
HgaI GACGC 4 cut(s) 485, 871, 1457, 2207
Hin1I GRCGYC 2 cut(s) 477, 863
Hin1II CATG 9 cut(s) 106, 703, 755, 784, 1051, 1259, 1389, 2228, 2632
HincII GTYRAC 3 cut(s) 838, 1276, 2554
HindII GTYRAC 3 cut(s) 838, 1276, 2554
HindIII AAGCTT 1 cut(s) 260
HpaI GTTAAC 1 cut(s) 1276
HpaII CCGG 8 cut(s) 554, 720, 866, 1458, 1493, 1930, 2178, 2703
Hpy166II GTNNAC 7 cut(s) 838, 1276, 1307, 1650, 2173, 2438, 2554
Hpy188I TCNGA 8 cut(s) 178, 624, 1202, 1473, 1736, 1743, 1849, 2286
Hpy8I GTNNAC 7 cut(s) 838, 1276, 1307, 1650, 2173, 2438, 2554
Hpy99I CGWCG 3 cut(s) 479, 800, 865
HpyCH4III ACNGT 4 cut(s) 717, 1489, 2049, 2612
HpyCH4IV ACGT 2 cut(s) 705, 1231
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 998, 1901, 2331
HpyF3I CTNAG 2 cut(s) 293, 2684
HpySE526I ACGT 2 cut(s) 705, 1231
Hsp92I GRCGYC 2 cut(s) 477, 863
Hsp92II CATG 9 cut(s) 106, 703, 755, 784, 1051, 1259, 1389, 2228, 2632
KflI GGGWCCC 2 cut(s) 684, 1980
Kpn2I TCCGGA 2 cut(s) 1457, 2177
Ksp22I TGATCA 3 cut(s) 64, 216, 2209
KspAI GTTAAC 1 cut(s) 1276
Lsp1109I GCAGC 5 cut(s) 1688, 1882, 2101, 2291, 2435
LweI GCATC 6 cut(s) 906, 1020, 1042, 1092, 1505, 1907
MaeI CTAG 6 cut(s) 1008, 1349, 1427, 1620, 1814, 1916
MaeII ACGT 2 cut(s) 705, 1231
MfeI CAATTG 1 cut(s) 141
MflI RGATCY 1 cut(s) 1806
MhlI GDGCHC 3 cut(s) 591, 1875, 2450
MlsI TGGCCA 1 cut(s) 1137
MluNI TGGCCA 1 cut(s) 1137
MlyI GAGTC 6 cut(s) 173, 305, 619, 1470, 1722, 2168
MmeI TCCRAC 6 cut(s) 519, 1496, 1729, 2175, 2499, 2658
Mox20I TGGCCA 1 cut(s) 1137
Mph1103I ATGCAT 1 cut(s) 2116
MroI TCCGGA 2 cut(s) 1457, 2177
MscI TGGCCA 1 cut(s) 1137
MseI TTAA 6 cut(s) 1094, 1275, 1479, 1583, 2562, 2719
MslI CAYNNNNRTG 5 cut(s) 107, 704, 1131, 1955, 2217
Msp20I TGGCCA 1 cut(s) 1137
MspI CCGG 8 cut(s) 554, 720, 866, 1458, 1493, 1930, 2178, 2703
MspR9I CCNGG 6 cut(s) 554, 1164, 1250, 1266, 1577, 1710
MunI CAATTG 1 cut(s) 141
Mva1269I GAATGC 1 cut(s) 2294
MvaI CCWGG 5 cut(s) 1164, 1250, 1266, 1577, 1710
MvnI CGCG 1 cut(s) 2232
MwoI GCNNNNNNNGC 4 cut(s) 28, 998, 1901, 2331
NciI CCSGG 1 cut(s) 554
NdeI CATATG 1 cut(s) 2116
NheI GCTAGC 1 cut(s) 1007
NlaIII CATG 9 cut(s) 106, 703, 755, 784, 1051, 1259, 1389, 2228, 2632
NlaIV GGNNCC 8 cut(s) 685, 686, 745, 1161, 1808, 1819, 1981, 1982
NmuCI GTSAC 5 cut(s) 711, 1591, 1688, 2076, 2696
NsiI ATGCAT 1 cut(s) 2116
NspI RCATGY 2 cut(s) 1259, 2228
NspV TTCGAA 2 cut(s) 301, 2298
PaeR7I CTCGAG 2 cut(s) 313, 2155
PaqCI CACCTGC 1 cut(s) 79
PceI AGGCCT 3 cut(s) 1920, 1996, 2264
PciI ACATGT 2 cut(s) 1255, 2224
PctI GAATGC 1 cut(s) 2294
PfeI GAWTC 6 cut(s) 179, 936, 1454, 1559, 1767, 1886
PfoI TCCNGGA 1 cut(s) 552
PinAI ACCGGT 2 cut(s) 1929, 2702
PkrI GCNGC 6 cut(s) 452, 1678, 1897, 2091, 2281, 2425
PleI GAGTC 6 cut(s) 172, 305, 619, 1469, 1722, 2168
PpsI GAGTC 6 cut(s) 172, 305, 619, 1469, 1722, 2168
PpuMI RGGWCCY 2 cut(s) 684, 1980
PscI ACATGT 2 cut(s) 1255, 2224
PshBI ATTAAT 1 cut(s) 1479
Psp124BI GAGCTC 2 cut(s) 1875, 2450
Psp5II RGGWCCY 2 cut(s) 684, 1980
Psp6I CCWGG 5 cut(s) 1162, 1248, 1264, 1575, 1708
PspEI GGTNACC 1 cut(s) 711
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 5 cut(s) 1162, 1248, 1264, 1575, 1708
PspN4I GGNNCC 8 cut(s) 685, 686, 745, 1161, 1808, 1819, 1981, 1982
PspPI GGNCC 7 cut(s) 239, 523, 684, 857, 1159, 1818, 1980
PspPPI RGGWCCY 2 cut(s) 684, 1980
PspXI VCTCGAGB 1 cut(s) 313
PstI CTGCAG 2 cut(s) 742, 1799
PstNI CAGNNNCTG 1 cut(s) 1895
PsuI RGATCY 1 cut(s) 1806
RsaI GTAC 6 cut(s) 530, 575, 1293, 1649, 2223, 2439
RsaNI GTAC 6 cut(s) 529, 574, 1292, 1648, 2222, 2438
RseI CAYNNNNRTG 5 cut(s) 107, 704, 1131, 1955, 2217
SacI GAGCTC 2 cut(s) 1875, 2450
SaqAI TTAA 6 cut(s) 1094, 1275, 1479, 1583, 2562, 2719
SatI GCNGC 6 cut(s) 451, 1677, 1896, 2090, 2280, 2424
Sau96I GGNCC 7 cut(s) 239, 523, 684, 857, 1159, 1818, 1980
SchI GAGTC 6 cut(s) 173, 305, 619, 1470, 1722, 2168
ScrFI CCNGG 6 cut(s) 554, 1164, 1250, 1266, 1577, 1710
SduI GDGCHC 3 cut(s) 591, 1875, 2450
SfaNI GCATC 6 cut(s) 906, 1020, 1042, 1092, 1505, 1907
SfcI CTRYAG 3 cut(s) 738, 1795, 2658
Sfr274I CTCGAG 2 cut(s) 313, 2155
SfuI TTCGAA 2 cut(s) 301, 2298
SgrAI CRCCGGYG 1 cut(s) 2702
SinI GGWCC 5 cut(s) 239, 523, 684, 1159, 1980
SlaI CTCGAG 2 cut(s) 313, 2155
SmiMI CAYNNNNRTG 5 cut(s) 107, 704, 1131, 1955, 2217
SmlI CTYRAG 3 cut(s) 313, 977, 2155
SmoI CTYRAG 3 cut(s) 313, 977, 2155
SseBI AGGCCT 3 cut(s) 1920, 1996, 2264
SsiI CCGC 7 cut(s) 448, 451, 828, 1864, 2204, 2230, 2325
SspMI CTAG 6 cut(s) 1008, 1349, 1427, 1620, 1814, 1916
SstI GAGCTC 2 cut(s) 1875, 2450
StuI AGGCCT 3 cut(s) 1920, 1996, 2264
StyD4I CCNGG 6 cut(s) 552, 1162, 1248, 1264, 1575, 1708
TaaI ACNGT 4 cut(s) 717, 1489, 2049, 2612
TaiI ACGT 2 cut(s) 708, 1234
TaqI TCGA 8 cut(s) 301, 314, 355, 383, 474, 2156, 2298, 2370
TatI WGTACW 5 cut(s) 528, 1291, 1647, 2221, 2437
TauI GCSGC 1 cut(s) 453
TfiI GAWTC 6 cut(s) 179, 936, 1454, 1559, 1767, 1886
Tru1I TTAA 6 cut(s) 1094, 1275, 1479, 1583, 2562, 2719
Tru9I TTAA 6 cut(s) 1094, 1275, 1479, 1583, 2562, 2719
TscAI CASTG 5 cut(s) 1600, 1664, 2169, 2341, 2650
TseFI GTSAC 5 cut(s) 711, 1591, 1688, 2076, 2696
TseI GCWGC 5 cut(s) 1676, 1895, 2089, 2279, 2423
Tsp45I GTSAC 5 cut(s) 711, 1591, 1688, 2076, 2696
TspDTI ATGAA 5 cut(s) 291, 886, 1391, 1526, 2500
TspGWI ACGGA 3 cut(s) 905, 1913, 2445
TspRI CASTG 5 cut(s) 1600, 1664, 2169, 2341, 2650
VpaK11BI GGWCC 5 cut(s) 239, 523, 684, 1159, 1980
VspI ATTAAT 1 cut(s) 1479
XagI CCTNNNNNAGG 2 cut(s) 980, 1020
XapI RAATTY 6 cut(s) 172, 297, 1720, 2101, 2408, 2572
XceI RCATGY 2 cut(s) 1259, 2228
XhoI CTCGAG 2 cut(s) 313, 2155
XmiI GTMKAC 1 cut(s) 2172
XspI CTAG 6 cut(s) 1008, 1349, 1427, 1620, 1814, 1916
Zsp2I ATGCAT 1 cut(s) 2116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.