Rmu_sc0008501.1_g000005

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008501.1
OG0000882
Physical Location & Seq
Reverse (-)
22534 .. 23187
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008501.1_g000005.1.cds

Sequence Viewer

Length: 654 bp
atgtcttgttcatcaggttttctaacagaaggagcaaattcagttaatgcatattttcctgctggaaactggtttgatctcttcaactactccagatcagtgagtgtagactccggagagtatgttacgttggatgcaccgcctgatcatatcaatgtacatgtccatgaaggaaacatattggccttgcaaggagaggccttgacaacacaagcagctcggaagactgcattcgaactcttggtggtcattagtgttagcggagagagcactggagaagtgttcttggatgatggagaggaagtcgaaatgggaggagagggagggaagtggagtttcgtgaggtttcgttctgcactagcacaaaatggaagtctagttttgagctcaaaggtcgaaaatggagaatttgcattaagccaaggttggatcattgataaaattaccgtcctcgggttggacaaagttaatgcaacagatgggtatacattgagcattactaaaggagcaaagttgaaagaaaacacaaatgttaaggccaactttcacagttatgagcgttttgtcaaggtagagtccagtgtatcccttcctataggaaaggagtttgagttggagctgaagccgaatcagatactggagagaacaccctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

217

Amino Acids

23.59

Weight (kDa)

4.83

Isoelectric Point (pI)

24.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 108, 485
AccIII TCCGGA 1 cut(s) 113
AciI CCGC 2 cut(s) 140, 261
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 2 cut(s) 37, 407
AcuI CTGAAG 1 cut(s) 641
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 2 cut(s) 453, 457
AflIII ACRYGT 1 cut(s) 160
AgsI TTSAA 2 cut(s) 85, 517
AluBI AGCT 3 cut(s) 218, 387, 619
AluI AGCT 3 cut(s) 218, 387, 619
Alw21I GWGCWC 2 cut(s) 272, 389
AlwI GGATC 1 cut(s) 437
AlwNI CAGNNNCTG 1 cut(s) 637
Ama87I CYCGRG 1 cut(s) 452
Aor13HI TCCGGA 1 cut(s) 113
AoxI GGCC 3 cut(s) 183, 198, 537
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 2 cut(s) 37, 407
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
AsuII TTCGAA 1 cut(s) 234
AvaI CYCGRG 1 cut(s) 452
BanII GRGCYC 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 2 cut(s) 272, 389
BbvI GCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 287, 473
BciVI GTATCC 1 cut(s) 595
BclI TGATCA 1 cut(s) 145
BfaI CTAG 3 cut(s) 359, 377, 652
BfmI CTRYAG 1 cut(s) 594
BfuI GTATCC 1 cut(s) 595
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 452
BmsI GCATC 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 2 cut(s) 76, 294
Bpu14I TTCGAA 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 421, 451
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 6 cut(s) 108, 138, 288, 318, 596, 626
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 457
Bse1I ACTGG 4 cut(s) 74, 277, 579, 642
BseAI TCCGGA 1 cut(s) 113
BseDI CCNNGG 2 cut(s) 421, 451
BseGI GGATG 2 cut(s) 139, 295
BseLI CCNNNNNNNGG 2 cut(s) 453, 457
BseNI ACTGG 4 cut(s) 74, 277, 579, 642
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 1 cut(s) 227
BsgI GTGCAG 1 cut(s) 339
BshFI GGCC 3 cut(s) 185, 200, 539
BsiHKAI GWGCWC 2 cut(s) 272, 389
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 1 cut(s) 114
BslI CCNNNNNNNGG 2 cut(s) 453, 457
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 3 cut(s) 185, 200, 539
BsoBI CYCGRG 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 272, 389
Bsp13I TCCGGA 1 cut(s) 113
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 4 cut(s) 76, 95, 145, 429
BspACI CCGC 2 cut(s) 140, 261
BspANI GGCC 3 cut(s) 185, 200, 539
BspEI TCCGGA 1 cut(s) 113
BspPI GGATC 1 cut(s) 437
BspT104I TTCGAA 1 cut(s) 234
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 4 cut(s) 74, 277, 579, 642
BssECI CCNNGG 2 cut(s) 421, 451
BssMI GATC 4 cut(s) 76, 95, 145, 429
BssNAI GTATAC 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 421
Bst1107I GTATAC 1 cut(s) 486
Bst4CI ACNGT 2 cut(s) 448, 551
Bst6I CTCTTC 1 cut(s) 86
BstAUI TGTACA 1 cut(s) 157
BstBI TTCGAA 1 cut(s) 234
BstF5I GGATG 2 cut(s) 139, 295
BstKTI GATC 4 cut(s) 79, 98, 148, 432
BstMBI GATC 4 cut(s) 76, 95, 145, 429
BstMWI GCNNNNNNNGC 1 cut(s) 267
BstNSI RCATGY 1 cut(s) 164
BstSFI CTRYAG 1 cut(s) 594
BstV1I GCAGC 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 230
BstZ17I GTATAC 1 cut(s) 486
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 3 cut(s) 185, 200, 539
BtsCI GGATG 2 cut(s) 139, 295
BtsIMutI CAGTG 3 cut(s) 105, 270, 586
CaiI CAGNNNCTG 1 cut(s) 637
Csp6I GTAC 1 cut(s) 158
CviAII CATG 2 cut(s) 161, 167
CviJI RGCY 8 cut(s) 185, 200, 218, 387, 420, 539, 619, 625
CviKI_1 RGCY 8 cut(s) 185, 200, 218, 387, 420, 539, 619, 625
CviQI GTAC 1 cut(s) 158
DpnI GATC 4 cut(s) 78, 97, 147, 431
DpnII GATC 4 cut(s) 76, 95, 145, 429
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Ecl136II GAGCTC 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 421
Eco147I AGGCCT 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 389
Eco53kI GAGCTC 1 cut(s) 387
Eco57I CTGAAG 1 cut(s) 641
Eco88I CYCGRG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 421
EcoT22I ATGCAT 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 389
ErhI CCWWGG 1 cut(s) 421
FaeI CATG 2 cut(s) 164, 170
FaiI YATR 9 cut(s) 52, 123, 150, 162, 168, 179, 486, 555, 596
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FatI CATG 2 cut(s) 160, 166
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 2 cut(s) 108, 485
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 146, 302
FriOI GRGCYC 1 cut(s) 389
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 3 cut(s) 359, 377, 652
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 2 cut(s) 76, 294
HaeIII GGCC 3 cut(s) 185, 200, 539
HapII CCGG 1 cut(s) 114
Hin1II CATG 2 cut(s) 164, 170
HinfI GANTC 3 cut(s) 110, 575, 628
HpaII CCGG 1 cut(s) 114
Hpy166II GTNNAC 2 cut(s) 109, 486
Hpy188I TCNGA 2 cut(s) 222, 633
Hpy188III TCNNGA 3 cut(s) 93, 114, 340
Hpy8I GTNNAC 2 cut(s) 109, 486
HpyAV CCTTC 3 cut(s) 23, 164, 599
HpyCH4III ACNGT 2 cut(s) 448, 551
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 7 cut(s) 50, 137, 190, 230, 356, 413, 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 267
HpySE526I ACGT 1 cut(s) 128
Hsp92II CATG 2 cut(s) 164, 170
Kpn2I TCCGGA 1 cut(s) 113
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 4 cut(s) 76, 95, 145, 429
LmnI GCTCC 3 cut(s) 32, 506, 616
LpnPI CCDG 9 cut(s) 48, 55, 72, 106, 127, 156, 258, 592, 623
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 1 cut(s) 124
MaeI CTAG 3 cut(s) 359, 377, 652
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 124
MalI GATC 4 cut(s) 78, 97, 147, 431
MboI GATC 4 cut(s) 76, 95, 145, 429
MboII GAAGA 2 cut(s) 73, 235
MhlI GDGCHC 2 cut(s) 272, 389
MluCI AATT 3 cut(s) 37, 407, 441
MlyI GAGTC 2 cut(s) 104, 584
MmeI TCCRAC 4 cut(s) 111, 407, 438, 594
MnlI CCTC 7 cut(s) 190, 292, 308, 313, 317, 336, 461
Mph1103I ATGCAT 1 cut(s) 52
MroI TCCGGA 1 cut(s) 113
MseI TTAA 4 cut(s) 45, 416, 468, 534
MslI CAYNNNNRTG 3 cut(s) 153, 165, 552
MspI CCGG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 267
NdeII GATC 4 cut(s) 76, 95, 145, 429
NlaIII CATG 2 cut(s) 164, 170
NsiI ATGCAT 1 cut(s) 52
NspI RCATGY 1 cut(s) 164
NspV TTCGAA 1 cut(s) 234
PceI AGGCCT 1 cut(s) 200
PciI ACATGT 1 cut(s) 160
PctI GAATGC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 628
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 2 cut(s) 104, 583
PpsI GAGTC 2 cut(s) 104, 583
PscI ACATGT 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 637
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
RseI CAYNNNNRTG 3 cut(s) 153, 165, 552
SacI GAGCTC 1 cut(s) 389
SaqAI TTAA 4 cut(s) 45, 416, 468, 534
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 4 cut(s) 76, 95, 145, 429
SchI GAGTC 2 cut(s) 104, 584
SduI GDGCHC 2 cut(s) 272, 389
SetI ASST 9 cut(s) 19, 131, 220, 347, 389, 396, 427, 573, 621
SfaNI GCATC 1 cut(s) 124
SfcI CTRYAG 1 cut(s) 594
SfuI TTCGAA 1 cut(s) 234
SmiMI CAYNNNNRTG 3 cut(s) 153, 165, 552
Sse9I AATT 3 cut(s) 37, 407, 441
SseBI AGGCCT 1 cut(s) 200
SsiI CCGC 2 cut(s) 140, 261
SspMI CTAG 3 cut(s) 359, 377, 652
SstI GAGCTC 1 cut(s) 389
StuI AGGCCT 1 cut(s) 200
StyI CCWWGG 1 cut(s) 421
TaaI ACNGT 2 cut(s) 448, 551
TaiI ACGT 1 cut(s) 131
TaqI TCGA 3 cut(s) 234, 306, 396
TasI AATT 3 cut(s) 37, 407, 441
TatI WGTACW 1 cut(s) 157
TfiI GAWTC 1 cut(s) 628
Tru1I TTAA 4 cut(s) 45, 416, 468, 534
Tru9I TTAA 4 cut(s) 45, 416, 468, 534
TscAI CASTG 3 cut(s) 105, 277, 586
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 183
TspRI CASTG 3 cut(s) 105, 277, 586
XapI RAATTY 2 cut(s) 37, 407
XceI RCATGY 1 cut(s) 164
XmiI GTMKAC 2 cut(s) 108, 485
XspI CTAG 3 cut(s) 359, 377, 652
Zsp2I ATGCAT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.