RLG00000032382

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000882
Physical Location & Seq
Forward (+)
14230801 .. 14236884
6084 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032382

Sequence Viewer

Length: 2685 bp
ATGGAAAGAAACAGATCAAATTATACACCAAAAGCAATATTGTTTCGTCATCTTCTACCATTCTTCTTCTTCTTGTCTCTGCTTGATTGTATAGTTGCAGAAGAACCAGTTGGGTATGGATACAAAGTCAAGTCTGTCAATTCTGATTCCTCCGGGAAGACATTGACGGCTAATCTTGGCCTGATCAAGAGCTCGTCAGTCTATGAGCCTGACATCCCAAATCTCAAGCTTTATGCCAGCTATGAAACCAAAGAAAGACTAAGAATTCGAATAACGGACTCCAATAACCAAAGATGGGAAATCCCTCAAGACCTTCTCCCACGCCAAACGCCACAAACATCCCACCATTCCATCCCCGAAAGCGACCTCATTTTCACACTCCACAACACCACCCCATTCGGCTTCACCGTCACCCGCCGTTCCTCCAACGACGTCGTATTCGACACCACCCCAAACCCCTCCAACCCTTCCTCCATCCTCATCTTCAAGGACCAGTACATCCAGCTTTCCTCCTCACTTCCGAAAACCCGGTCCTCCTTGTACGGCCTCGGTGAACACACCAAACCCTCCTTCAAGCTCCAACCCCATCAGACTCTGACACTCTGGACGGCCGACATTGCCAGTGCCAACCTCGACCTCAACCTCTATGGGTCCCACCCGTTTTACATGGACGTGAGGTCACCGTCAGGTGACGCAAAAGTTACCGCGGGAACCACTCACGGAGTGTTGCTGTTGAATAGTAATGGCATGGATGTAAGTTACGACGGTGACAGGGTTACTTATAAGGTGATTGGCGGAGTTGTTGACTTGTATTTGTTTTCGGGTCCAACGCCGGAATTGGTGATGAAGCAGTATACAGAGCTCATTGGCCGACCTGCCCCGATGCCCTACTGGTCTTTTGGATTCCACCAGTGTAGATATGGTTACAAGAATGTTGCTGATATTGAGGGGGTGGTTTCTGGTTATGCAAATGCTAGCATCCCTCTTGAAGTTATGTGGACAGACATTGACTACATGGATGCTTACAAGGATTTCACCCTTGATCCCATCAACTTTCCATTGGATAAGATGCAGAACTTTACAAACACCCTTCACCAAAATGGCCAGAAATATGTGCTCATCTTGGACCCTGGTATTAGTATAAATGAGAGCTATGGAACTTACATCCGAGGGAAGGAAGCAGATATATACATAAAACGTGATGGTATTCCTTACCAGGGAAATGTATGGCCTGGTGATGTTTACTTTCCTGATTTTGTACATCCACAAAGTGAACAATTTTGGGCTAATGAGATCAAACTATTTCAAGACCAACTAGCTTTTGATGGTCTCTGGCTTGATATGAATGAGGTATCCAATTTTATAACATCTCCTCCCACTCCGAATTCCACACTTGATGATCCACCTTACAAAATCAATGATTCCGGAGTCCAGCGTCCGATCATTAGTAAGACTGTTCCGGCATCAGCTCTACACTTTGGTAATATAACAGAGTATAACGTTCATAACCTATATGGGTTCTTGGAATCTAAAGCAACACACCAGGGGTTGATCAATGTCACTGGTAAGAGACCATTTATACTCGCTAGGTCAACCTTTGTAAGCTCTGGTAAGTACACAGCACACTGGACTGGCGACAATGCAGCAAGATGGAGTGACTTGGCATACACAATTCCAGGCATTTTGAATTTTGGACTCTTCGGAGTTCCGATGGTTGGAGCTGATATATGTGGTTTCTCTTTGAATACTACTGAAGAACTTTGCCGGCGATGGATTCAGCTAGGGGCCTTTTACCCGTTTTCACGAGATCATTCAGATAAGTTTACGATTCGCCAAGAGCTCTATCTTTGGGATTCAGTTGCTGCATCAGCAAGGAAGGTGCTAGGCCTCCGTTACCGGTTGCTTCCTTTGTTCTACACATCAATGTATCAGGCACACAAAAAAGGGACCCCCATTGCAAGGCCCCTTTTCTTCTCGTTTCCTGAAGATACCAATACATACGGCATCAATTCACAGTTCTTGATTGGTAAATGTGTGATGGTGTCACCTGTGTTGCAGGAAGGAGCAAATTCAGTTAATGCATATTTTCCTGCAGGAAACTGGTTTGATCTCTTCAACTTCTCCAGATCAGTGAGTGTAGACTCTGGCAAGTATGTTACGTTGGATGCACCGCCTGATCATATCAATGTACATGTCCATGAAGGAAACATATTGGCCTTGCAAGGAGAGGCCTTGACAACCCAAGCAGCTCGGAAGACTGCATTCGAACTCTTGGTCGTCATTAGTGTTAGAGGAGAGAGTACTGGAGAAGTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTCGTGAGGTTTCGTTCTGCACTAGCACAAAATGGAAGTCTAGTTTTGAGCTCAAAGGTCGAAAATGGAAAATTTGCATTAAGCCAAGGTTGGATCATTGATAAAATTACTGTCCTTGGGTGGGACAAAGTTAATGGAACAGATGGGTATACATTGAGCATTACTAAAGGAGCAAAGTTGAAAGGAAACACAAATGTTAAGGCCAACTTTCACAGTTATGAGCGTTTTGTCAAGGTAGAGTCCAGTGTATCCCTTCCTATAGGAAAGGAGTTTGAGTTAGAGCTTAAGCCGAACCAGATACTGGCGAAAACACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

895

Amino Acids

100.13

Weight (kDa)

6.14

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 87 - 246 1.7e-10 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 292 - 640 1.6e-120 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 649 - 738 6.7e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 783, 1364
AatII GACGTC 1 cut(s) 435
Acc36I ACCTGC 1 cut(s) 883
AccB7I CCANNNNNTGG 1 cut(s) 2668
AccI GTMKAC 3 cut(s) 854, 2139, 2516
AccII CGCG 1 cut(s) 707
AccIII TCCGGA 1 cut(s) 1424
AciI CCGC 5 cut(s) 415, 705, 707, 795, 2171
AclI AACGTT 1 cut(s) 1500
AclWI GGATC 3 cut(s) 1037, 1394, 2468
AcoI YGGCCR 3 cut(s) 609, 868, 1102
AcsI RAATTY 5 cut(s) 264, 1384, 1687, 2068, 2438
AcuI CTGAAG 2 cut(s) 1773, 2004
AcyI GRCGYC 1 cut(s) 432
AdeI CACNNNGTG 2 cut(s) 724, 1271
AfaI GTAC 6 cut(s) 497, 542, 1260, 1616, 2190, 2302
AfiI CCNNNNNNNGG 7 cut(s) 295, 1174, 1217, 1715, 1959, 2488, 2668
AflII CTTAAG 1 cut(s) 2651
AflIII ACRYGT 1 cut(s) 2191
AgeI ACCGGT 1 cut(s) 1896
AgsI TTSAA 9 cut(s) 487, 574, 736, 989, 1307, 1687, 1744, 2116, 2548
AhdI GACNNNNNGTC 1 cut(s) 676
AjiI CACGTC 1 cut(s) 673
AjnI CCWGG 5 cut(s) 1129, 1215, 1231, 1542, 1675
AjuI GAANNNNNNNTTGG 4 cut(s) 93, 125, 554, 586
Alw21I GWGCWC 5 cut(s) 194, 864, 1119, 1842, 2420
Alw26I GTCTC 3 cut(s) 81, 1334, 1564
AlwI GGATC 3 cut(s) 1037, 1394, 2468
AlwNI CAGNNNCTG 3 cut(s) 595, 1862, 2668
Aor13HI TCCGGA 1 cut(s) 1424
ApeKI GCWGC 3 cut(s) 1643, 1862, 2246
ApoI RAATTY 5 cut(s) 264, 1384, 1687, 2068, 2438
AsiGI ACCGGT 1 cut(s) 1896
AspS9I GGNCC 8 cut(s) 490, 531, 651, 824, 1126, 1785, 1947, 1962
AsuC2I CCSGG 2 cut(s) 154, 529
AsuII TTCGAA 2 cut(s) 268, 2265
AsuNHI GCTAGC 1 cut(s) 974
AvaII GGWCC 6 cut(s) 490, 531, 651, 824, 1126, 1947
BalI TGGCCA 1 cut(s) 1104
BanII GRGCYC 4 cut(s) 194, 864, 1842, 2420
BauI CACGAG 1 cut(s) 1803
BbsI GAAGAC 2 cut(s) 164, 2261
Bbv12I GWGCWC 5 cut(s) 194, 864, 1119, 1842, 2420
BbvI GCAGC 3 cut(s) 1655, 1849, 2258
BceAI ACGGC 5 cut(s) 183, 402, 559, 624, 2017
BciT130I CCWGG 5 cut(s) 1131, 1217, 1233, 1544, 1677
BciVI GTATCC 3 cut(s) 113, 1363, 2626
BclI TGATCA 3 cut(s) 183, 1551, 2176
BcnI CCSGG 2 cut(s) 154, 529
BcoDI GTCTC 3 cut(s) 81, 1334, 1564
BfaI CTAG 8 cut(s) 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683
BfmI CTRYAG 2 cut(s) 2091, 2625
BfrI CTTAAG 1 cut(s) 2651
BfuAI ACCTGC 1 cut(s) 883
BfuI GTATCC 3 cut(s) 113, 1363, 2626
BisI GCNGC 3 cut(s) 1644, 1863, 2247
BlsI GCNGC 3 cut(s) 1645, 1864, 2248
BmcAI AGTACT 1 cut(s) 2302
Bme1390I CCNGG 7 cut(s) 154, 529, 1131, 1217, 1233, 1544, 1677
Bme18I GGWCC 6 cut(s) 490, 531, 651, 824, 1126, 1947
BmeRI GACNNNNNGTC 1 cut(s) 676
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 8 cut(s) 490, 531, 651, 824, 1126, 1785, 1947, 1962
BmiI GGNNCC 9 cut(s) 652, 653, 712, 825, 1128, 1786, 1948, 1949, 1964
BmrFI CCNGG 7 cut(s) 154, 529, 1131, 1217, 1233, 1544, 1677
BmsI GCATC 8 cut(s) 873, 987, 1009, 1059, 1472, 1874, 2013, 2155
BmtI GCTAGC 1 cut(s) 978
BpiI GAAGAC 2 cut(s) 164, 2261
BpmI CTGGAG 2 cut(s) 2107, 2325
Bpu14I TTCGAA 2 cut(s) 268, 2265
BpuEI CTTGAG 2 cut(s) 209, 291
BpuMI CCSGG 2 cut(s) 154, 529
BsaHI GRCGYC 1 cut(s) 432
BsaI GGTCTC 2 cut(s) 1334, 1564
BsaJI CCNNGG 8 cut(s) 547, 705, 1129, 1168, 1216, 1543, 2452, 2482
BsaWI WCCGGW 2 cut(s) 1424, 1896
BsaXI ACNNNNNCTCC 8 cut(s) 455, 485, 1357, 1387, 2319, 2349, 2627, 2657
Bsc4I CCNNNNNNNGG 7 cut(s) 295, 1174, 1217, 1715, 1959, 2488, 2668
Bse118I RCCGGY 2 cut(s) 1764, 1896
Bse3DI GCAATG 2 cut(s) 615, 1953
BseAI TCCGGA 1 cut(s) 1424
BseBI CCWGG 5 cut(s) 1131, 1217, 1233, 1544, 1677
BseDI CCNNGG 8 cut(s) 547, 705, 1129, 1168, 1216, 1543, 2452, 2482
BseLI CCNNNNNNNGG 7 cut(s) 295, 1174, 1217, 1715, 1959, 2488, 2668
BseMI GCAATG 2 cut(s) 615, 1953
BseRI GAGGAG 4 cut(s) 502, 1362, 2307, 2361
BseX3I CGGCCG 1 cut(s) 609
BseXI GCAGC 3 cut(s) 1655, 1849, 2258
BsgI GTGCAG 1 cut(s) 2370
Bsh1236I CGCG 1 cut(s) 707
Bsh1285I CGRYCG 1 cut(s) 612
BshTI ACCGGT 1 cut(s) 1896
BsiEI CGRYCG 1 cut(s) 612
BsiHKAI GWGCWC 5 cut(s) 194, 864, 1119, 1842, 2420
BsiSI CCGG 7 cut(s) 153, 529, 833, 1425, 1460, 1765, 1897
BslFI GGGAC 3 cut(s) 637, 1960, 2504
BslI CCNNNNNNNGG 7 cut(s) 295, 1174, 1217, 1715, 1959, 2488, 2668
BsmAI GTCTC 3 cut(s) 81, 1334, 1564
BsmFI GGGAC 3 cut(s) 637, 1960, 2504
BsmI GAATGC 1 cut(s) 2261
Bso31I GGTCTC 2 cut(s) 1334, 1564
Bsp119I TTCGAA 2 cut(s) 268, 2265
Bsp1286I GDGCHC 5 cut(s) 194, 864, 1119, 1842, 2420
Bsp13I TCCGGA 1 cut(s) 1424
Bsp1407I TGTACA 2 cut(s) 1258, 2188
BspACI CCGC 5 cut(s) 415, 705, 707, 795, 2171
BspEI TCCGGA 1 cut(s) 1424
BspFNI CGCG 1 cut(s) 707
BspLI GGNNCC 9 cut(s) 652, 653, 712, 825, 1128, 1786, 1948, 1949, 1964
BspMAI CTGCAG 1 cut(s) 2095
BspMI ACCTGC 1 cut(s) 883
BspOI GCTAGC 1 cut(s) 978
BspPI GGATC 3 cut(s) 1037, 1394, 2468
BspT104I TTCGAA 2 cut(s) 268, 2265
BspTI CTTAAG 1 cut(s) 2651
BspTNI GGTCTC 2 cut(s) 1334, 1564
BsrDI GCAATG 2 cut(s) 615, 1953
BsrFI RCCGGY 2 cut(s) 1764, 1896
BsrGI TGTACA 2 cut(s) 1258, 2188
BssAI RCCGGY 2 cut(s) 1764, 1896
BssECI CCNNGG 8 cut(s) 547, 705, 1129, 1168, 1216, 1543, 2452, 2482
BssNAI GTATAC 2 cut(s) 855, 2517
BssNI GRCGYC 1 cut(s) 432
BssSI CACGAG 1 cut(s) 1803
BssT1I CCWWGG 2 cut(s) 2452, 2482
Bst1107I GTATAC 2 cut(s) 855, 2517
Bst2BI CACGAG 1 cut(s) 1803
Bst2UI CCWGG 5 cut(s) 1131, 1217, 1233, 1544, 1677
Bst4CI ACNGT 7 cut(s) 409, 684, 767, 1456, 2016, 2479, 2582
Bst6I CTCTTC 2 cut(s) 1703, 2117
BstACI GRCGYC 1 cut(s) 432
BstAFI CTTAAG 1 cut(s) 2651
BstAUI TGTACA 2 cut(s) 1258, 2188
BstBI TTCGAA 2 cut(s) 268, 2265
BstC8I GCNNGC 3 cut(s) 238, 976, 1766
BstDEI CTNAG 1 cut(s) 260
BstDSI CCRYGG 1 cut(s) 705
BstEII GGTNACC 1 cut(s) 678
BstFNI CGCG 1 cut(s) 707
BstMAI GTCTC 3 cut(s) 81, 1334, 1564
BstMCI CGRYCG 1 cut(s) 612
BstMWI GCNNNNNNNGC 2 cut(s) 617, 1868
BstNI CCWGG 5 cut(s) 1131, 1217, 1233, 1544, 1677
BstNSI RCATGY 1 cut(s) 2195
BstPI GGTNACC 1 cut(s) 678
BstSCI CCNGG 7 cut(s) 152, 527, 1129, 1215, 1231, 1542, 1675
BstSFI CTRYAG 2 cut(s) 2091, 2625
BstUI CGCG 1 cut(s) 707
BstV1I GCAGC 3 cut(s) 1655, 1849, 2258
BstV2I GAAGAC 2 cut(s) 164, 2261
BstZ17I GTATAC 2 cut(s) 855, 2517
BstZI CGGCCG 1 cut(s) 609
BsuI GTATCC 3 cut(s) 113, 1363, 2626
BtgI CCRYGG 1 cut(s) 705
BtgZI GCGATG 1 cut(s) 1783
BtrI CACGTC 1 cut(s) 673
BtsIMutI CAGTG 6 cut(s) 628, 917, 1560, 1624, 2136, 2617
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 3 cut(s) 238, 976, 1766
CaiI CAGNNNCTG 3 cut(s) 595, 1862, 2668
Cfr10I RCCGGY 2 cut(s) 1764, 1896
Cfr13I GGNCC 8 cut(s) 490, 531, 651, 824, 1126, 1785, 1947, 1962
Cfr42I CCGCGG 1 cut(s) 708
CseI GACGC 2 cut(s) 701, 1424
Csp6I GTAC 6 cut(s) 496, 541, 1259, 1615, 2189, 2301
CspAI ACCGGT 1 cut(s) 1896
CviAII CATG 5 cut(s) 667, 748, 1015, 2192, 2198
CviQI GTAC 6 cut(s) 496, 541, 1259, 1615, 2189, 2301
DdeI CTNAG 1 cut(s) 260
DraIII CACNNNGTG 2 cut(s) 724, 1271
DriI GACNNNNNGTC 1 cut(s) 676
EaeI YGGCCR 3 cut(s) 609, 868, 1102
EagI CGGCCG 1 cut(s) 609
Eam1104I CTCTTC 2 cut(s) 1703, 2117
Eam1105I GACNNNNNGTC 1 cut(s) 676
EarI CTCTTC 2 cut(s) 1703, 2117
EciI GGCGGA 1 cut(s) 810
Ecl136II GAGCTC 4 cut(s) 192, 862, 1840, 2418
EclXI CGGCCG 1 cut(s) 609
Eco130I CCWWGG 2 cut(s) 2452, 2482
Eco147I AGGCCT 2 cut(s) 1887, 2231
Eco24I GRGCYC 4 cut(s) 194, 864, 1842, 2420
Eco31I GGTCTC 2 cut(s) 1334, 1564
Eco47I GGWCC 6 cut(s) 490, 531, 651, 824, 1126, 1947
Eco52I CGGCCG 1 cut(s) 609
Eco53kI GAGCTC 4 cut(s) 192, 862, 1840, 2418
Eco57I CTGAAG 2 cut(s) 1773, 2004
Eco91I GGTNACC 1 cut(s) 678
EcoICRI GAGCTC 4 cut(s) 192, 862, 1840, 2418
EcoO109I RGGNCCY 4 cut(s) 651, 1785, 1947, 1962
EcoO65I GGTNACC 1 cut(s) 678
EcoRI GAATTC 2 cut(s) 264, 1384
EcoRII CCWGG 5 cut(s) 1129, 1215, 1231, 1542, 1675
EcoT14I CCWWGG 2 cut(s) 2452, 2482
EcoT22I ATGCAT 1 cut(s) 2083
EcoT38I GRGCYC 4 cut(s) 194, 864, 1842, 2420
ErhI CCWWGG 2 cut(s) 2452, 2482
FaeI CATG 5 cut(s) 670, 751, 1018, 2195, 2201
FalI AAGNNNNNCTT 2 cut(s) 2558, 2590
FaqI GGGAC 3 cut(s) 637, 1960, 2504
FatI CATG 5 cut(s) 666, 747, 1014, 2191, 2197
FauI CCCGC 2 cut(s) 422, 700
FbaI TGATCA 3 cut(s) 183, 1551, 2176
FblI GTMKAC 3 cut(s) 854, 2139, 2516
Fnu4HI GCNGC 3 cut(s) 1644, 1863, 2247
FriOI GRGCYC 4 cut(s) 194, 864, 1842, 2420
Fsp4HI GCNGC 3 cut(s) 1644, 1863, 2247
FspBI CTAG 8 cut(s) 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683
GluI GCNGC 3 cut(s) 1644, 1863, 2247
GsuI CTGGAG 2 cut(s) 2107, 2325
HapII CCGG 7 cut(s) 153, 529, 833, 1425, 1460, 1765, 1897
HgaI GACGC 2 cut(s) 701, 1424
Hin1I GRCGYC 1 cut(s) 432
Hin1II CATG 5 cut(s) 670, 751, 1018, 2195, 2201
HincII GTYRAC 2 cut(s) 805, 1593
HindII GTYRAC 2 cut(s) 805, 1593
HindIII AAGCTT 1 cut(s) 227
HpaII CCGG 7 cut(s) 153, 529, 833, 1425, 1460, 1765, 1897
Hpy99I CGWCG 3 cut(s) 434, 437, 767
HpyAV CCTTC 9 cut(s) 323, 477, 580, 1100, 1168, 1870, 2054, 2195, 2630
HpyCH4III ACNGT 7 cut(s) 409, 684, 767, 1456, 2016, 2479, 2582
HpyCH4IV ACGT 5 cut(s) 432, 672, 1198, 1500, 2159
HpyF10VI GCNNNNNNNGC 2 cut(s) 617, 1868
HpyF3I CTNAG 1 cut(s) 260
HpySE526I ACGT 5 cut(s) 432, 672, 1198, 1500, 2159
Hsp92I GRCGYC 1 cut(s) 432
Hsp92II CATG 5 cut(s) 670, 751, 1018, 2195, 2201
KflI GGGWCCC 2 cut(s) 651, 1947
Kpn2I TCCGGA 1 cut(s) 1424
KroI GCCGGC 1 cut(s) 1764
KroNI GCCGGC 1 cut(s) 1766
Ksp22I TGATCA 3 cut(s) 183, 1551, 2176
KspI CCGCGG 1 cut(s) 708
LmnI GCTCC 4 cut(s) 582, 1718, 2063, 2537
Lsp1109I GCAGC 3 cut(s) 1655, 1849, 2258
LweI GCATC 8 cut(s) 873, 987, 1009, 1059, 1472, 1874, 2013, 2155
MaeI CTAG 8 cut(s) 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683
MaeII ACGT 5 cut(s) 432, 672, 1198, 1500, 2159
MhlI GDGCHC 5 cut(s) 194, 864, 1119, 1842, 2420
MlsI TGGCCA 1 cut(s) 1104
MluNI TGGCCA 1 cut(s) 1104
MlyI GAGTC 6 cut(s) 272, 586, 1437, 1689, 2135, 2615
MmeI TCCRAC 7 cut(s) 450, 486, 604, 851, 1696, 2142, 2438
Mox20I TGGCCA 1 cut(s) 1104
Mph1103I ATGCAT 1 cut(s) 2083
MroI TCCGGA 1 cut(s) 1424
MroNI GCCGGC 1 cut(s) 1764
MscI TGGCCA 1 cut(s) 1104
MseI TTAA 5 cut(s) 2076, 2447, 2499, 2565, 2652
MslI CAYNNNNRTG 6 cut(s) 671, 1098, 1922, 2184, 2196, 2583
Msp20I TGGCCA 1 cut(s) 1104
MspA1I CMGCKG 1 cut(s) 707
MspCI CTTAAG 1 cut(s) 2651
MspI CCGG 7 cut(s) 153, 529, 833, 1425, 1460, 1765, 1897
MspR9I CCNGG 7 cut(s) 154, 529, 1131, 1217, 1233, 1544, 1677
Mva1269I GAATGC 1 cut(s) 2261
MvaI CCWGG 5 cut(s) 1131, 1217, 1233, 1544, 1677
MvnI CGCG 1 cut(s) 707
MwoI GCNNNNNNNGC 2 cut(s) 617, 1868
NaeI GCCGGC 1 cut(s) 1766
NciI CCSGG 2 cut(s) 154, 529
NgoMIV GCCGGC 1 cut(s) 1764
NheI GCTAGC 1 cut(s) 974
NlaIII CATG 5 cut(s) 670, 751, 1018, 2195, 2201
NlaIV GGNNCC 9 cut(s) 652, 653, 712, 825, 1128, 1786, 1948, 1949, 1964
NmuCI GTSAC 7 cut(s) 409, 678, 689, 767, 1558, 1655, 2043
NsiI ATGCAT 1 cut(s) 2083
NspI RCATGY 1 cut(s) 2195
NspV TTCGAA 2 cut(s) 268, 2265
PceI AGGCCT 2 cut(s) 1887, 2231
PciI ACATGT 1 cut(s) 2191
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 438
PctI GAATGC 1 cut(s) 2261
PdiI GCCGGC 1 cut(s) 1766
PfeI GAWTC 7 cut(s) 146, 903, 1421, 1526, 1774, 1828, 1853
PflMI CCANNNNNTGG 1 cut(s) 2668
PfoI TCCNGGA 1 cut(s) 152
PinAI ACCGGT 1 cut(s) 1896
PkrI GCNGC 3 cut(s) 1645, 1864, 2248
PleI GAGTC 6 cut(s) 272, 586, 1436, 1689, 2135, 2614
PpsI GAGTC 6 cut(s) 272, 586, 1436, 1689, 2135, 2614
PpuMI RGGWCCY 2 cut(s) 651, 1947
PscI ACATGT 1 cut(s) 2191
PsiI TTATAA 2 cut(s) 783, 1364
Psp124BI GAGCTC 4 cut(s) 194, 864, 1842, 2420
Psp1406I AACGTT 1 cut(s) 1500
Psp5II RGGWCCY 2 cut(s) 651, 1947
Psp6I CCWGG 5 cut(s) 1129, 1215, 1231, 1542, 1675
PspEI GGTNACC 1 cut(s) 678
PspGI CCWGG 5 cut(s) 1129, 1215, 1231, 1542, 1675
PspN4I GGNNCC 9 cut(s) 652, 653, 712, 825, 1128, 1786, 1948, 1949, 1964
PspPI GGNCC 8 cut(s) 490, 531, 651, 824, 1126, 1785, 1947, 1962
PspPPI RGGWCCY 2 cut(s) 651, 1947
PstI CTGCAG 1 cut(s) 2095
PstNI CAGNNNCTG 3 cut(s) 595, 1862, 2668
RsaI GTAC 6 cut(s) 497, 542, 1260, 1616, 2190, 2302
RsaNI GTAC 6 cut(s) 496, 541, 1259, 1615, 2189, 2301
RseI CAYNNNNRTG 6 cut(s) 671, 1098, 1922, 2184, 2196, 2583
SacI GAGCTC 4 cut(s) 194, 864, 1842, 2420
SacII CCGCGG 1 cut(s) 708
SaqAI TTAA 5 cut(s) 2076, 2447, 2499, 2565, 2652
SatI GCNGC 3 cut(s) 1644, 1863, 2247
Sau96I GGNCC 8 cut(s) 490, 531, 651, 824, 1126, 1785, 1947, 1962
SbfI CCTGCAGG 1 cut(s) 2095
ScaI AGTACT 1 cut(s) 2302
SchI GAGTC 6 cut(s) 272, 586, 1437, 1689, 2135, 2615
ScrFI CCNGG 7 cut(s) 154, 529, 1131, 1217, 1233, 1544, 1677
SdaI CCTGCAGG 1 cut(s) 2095
SduI GDGCHC 5 cut(s) 194, 864, 1119, 1842, 2420
SfaNI GCATC 8 cut(s) 873, 987, 1009, 1059, 1472, 1874, 2013, 2155
SfcI CTRYAG 2 cut(s) 2091, 2625
Sfr303I CCGCGG 1 cut(s) 708
SfuI TTCGAA 2 cut(s) 268, 2265
SgrBI CCGCGG 1 cut(s) 708
SinI GGWCC 6 cut(s) 490, 531, 651, 824, 1126, 1947
SmiMI CAYNNNNRTG 6 cut(s) 671, 1098, 1922, 2184, 2196, 2583
SmlI CTYRAG 3 cut(s) 224, 306, 2651
SmoI CTYRAG 3 cut(s) 224, 306, 2651
Sse8387I CCTGCAGG 1 cut(s) 2095
SseBI AGGCCT 2 cut(s) 1887, 2231
SsiI CCGC 5 cut(s) 415, 705, 707, 795, 2171
SspI AATATT 1 cut(s) 39
SspMI CTAG 8 cut(s) 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683
SstI GAGCTC 4 cut(s) 194, 864, 1842, 2420
StuI AGGCCT 2 cut(s) 1887, 2231
StyD4I CCNGG 7 cut(s) 152, 527, 1129, 1215, 1231, 1542, 1675
StyI CCWWGG 2 cut(s) 2452, 2482
TaaI ACNGT 7 cut(s) 409, 684, 767, 1456, 2016, 2479, 2582
TaiI ACGT 5 cut(s) 435, 675, 1201, 1503, 2162
TaqI TCGA 6 cut(s) 268, 441, 633, 2265, 2337, 2427
TatI WGTACW 5 cut(s) 495, 1258, 1614, 2188, 2300
TfiI GAWTC 7 cut(s) 146, 903, 1421, 1526, 1774, 1828, 1853
Tru1I TTAA 5 cut(s) 2076, 2447, 2499, 2565, 2652
Tru9I TTAA 5 cut(s) 2076, 2447, 2499, 2565, 2652
TscAI CASTG 6 cut(s) 628, 917, 1567, 1631, 2136, 2617
TseFI GTSAC 7 cut(s) 409, 678, 689, 767, 1558, 1655, 2043
TseI GCWGC 3 cut(s) 1643, 1862, 2246
Tsp45I GTSAC 7 cut(s) 409, 678, 689, 767, 1558, 1655, 2043
TspDTI ATGAA 5 cut(s) 258, 860, 1358, 1493, 2214
TspGWI ACGGA 3 cut(s) 290, 735, 1880
TspRI CASTG 6 cut(s) 628, 917, 1567, 1631, 2136, 2617
Van91I CCANNNNNTGG 1 cut(s) 2668
Vha464I CTTAAG 1 cut(s) 2651
VpaK11BI GGWCC 6 cut(s) 490, 531, 651, 824, 1126, 1947
XapI RAATTY 5 cut(s) 264, 1384, 1687, 2068, 2438
XceI RCATGY 1 cut(s) 2195
XcmI CCANNNNNNNNNTGG 1 cut(s) 917
XmiI GTMKAC 3 cut(s) 854, 2139, 2516
XspI CTAG 8 cut(s) 975, 1316, 1587, 1781, 1883, 2390, 2408, 2683
ZraI GACGTC 1 cut(s) 433
ZrmI AGTACT 1 cut(s) 2302
Zsp2I ATGCAT 1 cut(s) 2083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.