Rw5G012110

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000882
Physical Location & Seq
Forward (+)
14186867 .. 14192008
5142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012110.1

Sequence Viewer

Length: 2682 bp
ATGGAAAAAAATAGTTCAGATTATACATTTCATCATCTTCTACCATTCTTCTTCTTCTTCTTCTTCATTTCTCAGCTACATTGTTTAGTTGCAGAAGAACCAGTTGGGTATGGATATAAAGTTGAGTCTGTGAATTATGATTCCTCTGGGAAGACATTGATGGCTAATCTTGGCCTGATCAATAGCTCCTCAATCTATGGACCTGACATCCCAAATCTAAAGCTTTATGCCAGCTATGAAACCAAAGAAAGACTAAGAATTCGAATAACAGACTCGAGTAACCAAAGATGGGAAATCCCACAAGACCTTCTCCCACGACAAACTCCACAAACATCCCACCATTCGATCCCCGAAAACGACCTCATTTTCACCCTCCACACCACCACCCCGTTCGGCTTCACGGTCACCCGCCGCTTCTCCAATGATGTCGTATTCGACGCCACCCCAAACCCCTCCAACCCATCCTCCATCCTCGTCTTCAAGGACCAGTACATCCAGCTTTCCTCCTCTCTCCCGGAAACCCGATCTTCCCTGTACGGCCTCGGTGAGCACACCAAAGCCTCCTTCAAGCTCCAATCCAATCAGACTCTAACACTCTGGACTGCCGACATTTTCAGCTCCATCCCCGACCTCAATCTCTATGGGTCCCACCCATTTTACATGGACGTGAGGTCACCGTCCGGTGATGAAAAAGTTACTGCAGGAACCACTCATGGAGTTTTGCTATTGAATAGTAATGGCATGGATGTAAATTACGACGGCGACAGGATTACATATAAAGTGATTGGCGGAGTTGTTGACTTGTATTTTTTTTCTGGGCCGACGCCGGAATTGGTGATGGAGCAATACACGGAGTTCATTGGCCGACCTGCCCCGATGCCCTACTGGTCTTTTGGATTCCACCAATGTAGATATAGTTACAAGGATGTTGCTGACCTTGAGGAGGTGGTTGCTGGTTATGCAAATGCTAGCATCCCTCTTGAAGGTATTTGGACAGACATTGACTACATGGATGCTTACAAGGATTTCACTCTTGATCCCATCAACTTTCCATTAAGTAAGATGCAGAACTTTACAAGCACCCTTCACCAAAATGGCCAGAAATATGTGCCGATCTTGGACCCTGGTATTAGTATAAATGAGAGCTATGGAAGTTACATCAGAGGGAAGGAAGCAGATATATACATAAAACGTGATGGTATTCCTTACCAGGGACATGTATGGCCTGGTGATGTTAACTATCCTGATTTTGTACATCCACAAAGTGAACAATTTTGGGCTAATGAGATCAAACTATTTCAAGACCAACTAGCTTTTGATGGTCTTTGGCTTGATATGAATGAGCCATGCAATTTGATAACATCTGCTCCCACTCCATATTCTACACTAGATGATCCACCTTACAAAATCAATGATTCCGGAGTCCAGCGTCCGACCATTAATAAGACTGTTCCGGCATCAGCTCTACACTTTGGTAATATAACAGAGTATAATGTTCATAACCTATATGGGTTCTTGGAATCTAAAGCAACACACCAGGGGTTAATCAATGTCACTGGTAAGAGACCATTTATACTCACTAGATCAACCTTTGTAAGCTCTGGTAAGTACACAGCACACTGGACTGGAGACAATGCAGCAAGATGGAGTGACTTGGCATACACAATTCCAGGCATTTTGAATTTTGGACTCTTCGGAGTTCCGATGGTTGGAGCTGATATATGTGGATTCACTTTGAATACTACTGAAGAACTCTGTAGGCGATGGATCCAGCTAGGGGCCTTTTATCCTTTTGCAAGAGATCATTCAGATATATTTACAATCCGCCAAGAGCTCTATGTTTGGGATTCAGTTGCTGCATCAGCAAGGAAGGTGCTAGGCCTCCGTTACCGGTTGCTCCCCTTGTTCTACACATCAATGTATCAGGCACACAAAAAAGGGACCCCCATTGCAAGGCCTCTTTTCTTCTCGTTTCCTGAAGATACCAATACATACAACATCAATTCACAATTCTTGATTGGTAAAGGTGTGATGGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTTATGCATATGTTCCTGCTGGAAACTGGTTTGATCTCTTCAACTACTCGAGATCAGTGAGTGTAGACTCCGGAGAGTATGTTATGTTGGACGCACCGCCTGATCATATCAATGTACATGTCCGCGAAGGAAACATTTTGGCCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCTCGGAAGACTGCATTCGAACTTTTGGTGGTCATTAGTAGTAGCGGAGATAGCACTGGAGAAGTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTAGTGAAATTCTATTGTGGAGCAGCAAACGGAAGTGTACTTTTGAGCTCAAAAGTTGAAAATGGAGGATTTGCTTTGAGCCAAAAATGGATCATTGATAAAGTTACCTTCATTGGGTTGGAAAAAGTTGATGGATTAGAAGGGTTTGCAGTCAACTTTATTAAGGGAACAAATTTGAAAGGAATGTGGGATGTCAATGCCAACTTTCACGGTGATAAGCGTTTTGTCATGGTAAAAATATCCAGTGTATCCATTCCTATAGGAAAAGAGTTTGAGTTGGAGCTGAGCCTGAAGCGTGACACCGGTGAAAACACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

893

Amino Acids

100.14

Weight (kDa)

5.24

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 85 - 245 1.6e-09 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 290 - 638 5.3e-118 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 647 - 736 2.1e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 877
AccI GTMKAC 1 cut(s) 2133
AccII CGCG 1 cut(s) 2193
AccIII TCCGGA 2 cut(s) 1418, 2138
AciI CCGC 7 cut(s) 409, 412, 789, 1825, 2165, 2191, 2286
AclWI GGATC 6 cut(s) 340, 1031, 1388, 1762, 1775, 2459
AcoI YGGCCR 2 cut(s) 862, 1096
AcsI RAATTY 5 cut(s) 258, 1681, 2062, 2369, 2533
AcuI CTGAAG 3 cut(s) 1767, 1998, 2672
AcyI GRCGYC 2 cut(s) 438, 824
AdeI CACNNNGTG 1 cut(s) 1265
AfaI GTAC 6 cut(s) 491, 536, 1254, 1610, 2184, 2400
AfiI CCNNNNNNNGG 8 cut(s) 289, 943, 983, 1211, 1709, 1777, 1953, 2475
AflIII ACRYGT 2 cut(s) 1216, 2185
AgeI ACCGGT 2 cut(s) 1890, 2663
AhdI GACNNNNNGTC 1 cut(s) 670
AjiI CACGTC 1 cut(s) 667
AjnI CCWGG 5 cut(s) 1123, 1209, 1225, 1536, 1669
AjuI GAANNNNNNNTTGG 4 cut(s) 87, 119, 548, 580
Alw21I GWGCWC 3 cut(s) 552, 1836, 2411
Alw26I GTCTC 2 cut(s) 1558, 1623
AlwI GGATC 6 cut(s) 340, 1031, 1388, 1762, 1775, 2459
AlwNI CAGNNNCTG 1 cut(s) 1856
Ama87I CYCGRG 2 cut(s) 274, 2116
Aor13HI TCCGGA 2 cut(s) 1418, 2138
ApeKI GCWGC 5 cut(s) 1637, 1856, 2050, 2240, 2384
ApoI RAATTY 5 cut(s) 258, 1681, 2062, 2369, 2533
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AseI ATTAAT 1 cut(s) 1440
AsiGI ACCGGT 2 cut(s) 1890, 2663
AspS9I GGNCC 7 cut(s) 200, 484, 645, 818, 1120, 1779, 1941
AsuC2I CCSGG 1 cut(s) 515
AsuII TTCGAA 2 cut(s) 262, 2259
AsuNHI GCTAGC 1 cut(s) 968
AvaI CYCGRG 2 cut(s) 274, 2116
AvaII GGWCC 5 cut(s) 200, 484, 645, 1120, 1941
BalI TGGCCA 1 cut(s) 1098
BamHI GGATCC 1 cut(s) 1767
BanII GRGCYC 2 cut(s) 1836, 2411
BbsI GAAGAC 3 cut(s) 158, 469, 2255
Bbv12I GWGCWC 3 cut(s) 552, 1836, 2411
BbvI GCAGC 5 cut(s) 1649, 1843, 2062, 2252, 2396
BceAI ACGGC 2 cut(s) 553, 775
BciT130I CCWGG 5 cut(s) 1125, 1211, 1227, 1538, 1671
BciVI GTATCC 1 cut(s) 2620
BclI TGATCA 2 cut(s) 177, 2170
BcnI CCSGG 1 cut(s) 515
BcoDI GTCTC 2 cut(s) 1558, 1623
BfaI CTAG 6 cut(s) 969, 1310, 1388, 1581, 1775, 1877
BfmI CTRYAG 3 cut(s) 699, 1756, 2619
BfuAI ACCTGC 1 cut(s) 877
BfuI GTATCC 1 cut(s) 2620
BisI GCNGC 6 cut(s) 412, 1638, 1857, 2051, 2241, 2385
BlpI GCTNAGC 1 cut(s) 2645
BlsI GCNGC 6 cut(s) 413, 1639, 1858, 2052, 2242, 2386
Bme1390I CCNGG 6 cut(s) 515, 1125, 1211, 1227, 1538, 1671
Bme18I GGWCC 5 cut(s) 200, 484, 645, 1120, 1941
BmeRI GACNNNNNGTC 1 cut(s) 670
BmeT110I CYCGRG 2 cut(s) 274, 2116
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 7 cut(s) 200, 484, 645, 818, 1120, 1779, 1941
BmiI GGNNCC 8 cut(s) 646, 647, 706, 1122, 1769, 1780, 1942, 1943
BmrFI CCNGG 6 cut(s) 515, 1125, 1211, 1227, 1538, 1671
BmsI GCATC 6 cut(s) 867, 981, 1003, 1053, 1466, 1868
BmtI GCTAGC 1 cut(s) 972
BpiI GAAGAC 3 cut(s) 158, 469, 2255
BpmI CTGGAG 2 cut(s) 1647, 2319
Bpu1102I GCTNAGC 1 cut(s) 2645
Bpu14I TTCGAA 2 cut(s) 262, 2259
BpuEI CTTGAG 1 cut(s) 959
BpuMI CCSGG 1 cut(s) 515
BsaHI GRCGYC 2 cut(s) 438, 824
BsaI GGTCTC 1 cut(s) 1558
BsaJI CCNNGG 4 cut(s) 541, 1123, 1210, 1537
BsaWI WCCGGW 5 cut(s) 680, 1418, 1890, 2138, 2663
BsaXI ACNNNNNCTCC 8 cut(s) 449, 479, 1351, 1381, 2133, 2163, 2313, 2343
Bsc4I CCNNNNNNNGG 8 cut(s) 289, 943, 983, 1211, 1709, 1777, 1953, 2475
Bse118I RCCGGY 2 cut(s) 1890, 2663
Bse1I ACTGG 9 cut(s) 101, 487, 890, 1561, 1625, 1630, 2099, 2302, 2604
Bse3DI GCAATG 1 cut(s) 1947
BseAI TCCGGA 2 cut(s) 1418, 2138
BseBI CCWGG 5 cut(s) 1125, 1211, 1227, 1538, 1671
BseDI CCNNGG 4 cut(s) 541, 1123, 1210, 1537
BseLI CCNNNNNNNGG 8 cut(s) 289, 943, 983, 1211, 1709, 1777, 1953, 2475
BseMI GCAATG 1 cut(s) 1947
BseMII CTCAG 2 cut(s) 86, 2636
BseNI ACTGG 9 cut(s) 101, 487, 890, 1561, 1625, 1630, 2099, 2302, 2604
BseRI GAGGAG 4 cut(s) 178, 496, 956, 2355
BseXI GCAGC 5 cut(s) 1649, 1843, 2062, 2252, 2396
Bsh1236I CGCG 1 cut(s) 2193
BshTI ACCGGT 2 cut(s) 1890, 2663
BsiHKAI GWGCWC 3 cut(s) 552, 1836, 2411
BsiHKCI CYCGRG 2 cut(s) 274, 2116
BsiSI CCGG 8 cut(s) 515, 681, 827, 1419, 1454, 1891, 2139, 2664
BslFI GGGAC 3 cut(s) 631, 1227, 1954
BslI CCNNNNNNNGG 8 cut(s) 289, 943, 983, 1211, 1709, 1777, 1953, 2475
BsmAI GTCTC 2 cut(s) 1558, 1623
BsmFI GGGAC 3 cut(s) 631, 1227, 1954
BsmI GAATGC 1 cut(s) 2255
Bso31I GGTCTC 1 cut(s) 1558
BsoBI CYCGRG 2 cut(s) 274, 2116
Bsp119I TTCGAA 2 cut(s) 262, 2259
Bsp1286I GDGCHC 3 cut(s) 552, 1836, 2411
Bsp13I TCCGGA 2 cut(s) 1418, 2138
Bsp1407I TGTACA 2 cut(s) 1252, 2182
Bsp1720I GCTNAGC 1 cut(s) 2645
BspACI CCGC 7 cut(s) 409, 412, 789, 1825, 2165, 2191, 2286
BspCNI CTCAG 2 cut(s) 85, 2637
BspEI TCCGGA 2 cut(s) 1418, 2138
BspFNI CGCG 1 cut(s) 2193
BspLI GGNNCC 8 cut(s) 646, 647, 706, 1122, 1769, 1780, 1942, 1943
BspMAI CTGCAG 1 cut(s) 703
BspMI ACCTGC 1 cut(s) 877
BspOI GCTAGC 1 cut(s) 972
BspPI GGATC 6 cut(s) 340, 1031, 1388, 1762, 1775, 2459
BspT104I TTCGAA 2 cut(s) 262, 2259
BspTNI GGTCTC 1 cut(s) 1558
BsrDI GCAATG 1 cut(s) 1947
BsrFI RCCGGY 2 cut(s) 1890, 2663
BsrGI TGTACA 2 cut(s) 1252, 2182
BsrI ACTGG 9 cut(s) 101, 487, 890, 1561, 1625, 1630, 2099, 2302, 2604
BssAI RCCGGY 2 cut(s) 1890, 2663
BssECI CCNNGG 4 cut(s) 541, 1123, 1210, 1537
BssNI GRCGYC 2 cut(s) 438, 824
Bst2UI CCWGG 5 cut(s) 1125, 1211, 1227, 1538, 1671
Bst4CI ACNGT 4 cut(s) 403, 678, 1450, 2573
Bst6I CTCTTC 2 cut(s) 1697, 2111
BstACI GRCGYC 2 cut(s) 438, 824
BstAUI TGTACA 2 cut(s) 1252, 2182
BstBI TTCGAA 2 cut(s) 262, 2259
BstC8I GCNNGC 2 cut(s) 232, 970
BstDEI CTNAG 3 cut(s) 72, 254, 2645
BstEII GGTNACC 2 cut(s) 403, 672
BstENI CCTNNNNNAGG 2 cut(s) 941, 981
BstFNI CGCG 1 cut(s) 2193
BstMAI GTCTC 2 cut(s) 1558, 1623
BstMWI GCNNNNNNNGC 3 cut(s) 959, 1862, 2292
BstNI CCWGG 5 cut(s) 1125, 1211, 1227, 1538, 1671
BstNSI RCATGY 2 cut(s) 1220, 2189
BstPI GGTNACC 2 cut(s) 403, 672
BstSCI CCNGG 6 cut(s) 513, 1123, 1209, 1225, 1536, 1669
BstSFI CTRYAG 3 cut(s) 699, 1756, 2619
BstUI CGCG 1 cut(s) 2193
BstV1I GCAGC 5 cut(s) 1649, 1843, 2062, 2252, 2396
BstV2I GAAGAC 3 cut(s) 158, 469, 2255
BstX2I RGATCY 1 cut(s) 1767
BstYI RGATCY 1 cut(s) 1767
BsuI GTATCC 1 cut(s) 2620
BtgZI GCGATG 1 cut(s) 1777
BtrI CACGTC 1 cut(s) 667
BtsIMutI CAGTG 5 cut(s) 1554, 1618, 2130, 2295, 2611
BveI ACCTGC 1 cut(s) 877
Cac8I GCNNGC 2 cut(s) 232, 970
CaiI CAGNNNCTG 1 cut(s) 1856
Cfr10I RCCGGY 2 cut(s) 1890, 2663
Cfr13I GGNCC 7 cut(s) 200, 484, 645, 818, 1120, 1779, 1941
CseI GACGC 4 cut(s) 446, 832, 1418, 2168
Csp6I GTAC 6 cut(s) 490, 535, 1253, 1609, 2183, 2399
CspAI ACCGGT 2 cut(s) 1890, 2663
CviAII CATG 8 cut(s) 661, 713, 742, 1009, 1217, 1347, 2186, 2590
CviQI GTAC 6 cut(s) 490, 535, 1253, 1609, 2183, 2399
DdeI CTNAG 3 cut(s) 72, 254, 2645
DraIII CACNNNGTG 1 cut(s) 1265
DriI GACNNNNNGTC 1 cut(s) 670
EaeI YGGCCR 2 cut(s) 862, 1096
Eam1104I CTCTTC 2 cut(s) 1697, 2111
Eam1105I GACNNNNNGTC 1 cut(s) 670
EarI CTCTTC 2 cut(s) 1697, 2111
EciI GGCGGA 2 cut(s) 804, 1814
Ecl136II GAGCTC 2 cut(s) 1834, 2409
Eco147I AGGCCT 3 cut(s) 1881, 1957, 2225
Eco24I GRGCYC 2 cut(s) 1836, 2411
Eco31I GGTCTC 1 cut(s) 1558
Eco47I GGWCC 5 cut(s) 200, 484, 645, 1120, 1941
Eco53kI GAGCTC 2 cut(s) 1834, 2409
Eco57I CTGAAG 3 cut(s) 1767, 1998, 2672
Eco88I CYCGRG 2 cut(s) 274, 2116
Eco91I GGTNACC 2 cut(s) 403, 672
EcoICRI GAGCTC 2 cut(s) 1834, 2409
EcoNI CCTNNNNNAGG 2 cut(s) 941, 981
EcoO109I RGGNCCY 3 cut(s) 645, 1779, 1941
EcoO65I GGTNACC 2 cut(s) 403, 672
EcoRI GAATTC 1 cut(s) 258
EcoRII CCWGG 5 cut(s) 1123, 1209, 1225, 1536, 1669
EcoT22I ATGCAT 1 cut(s) 2077
EcoT38I GRGCYC 2 cut(s) 1836, 2411
FaeI CATG 8 cut(s) 664, 716, 745, 1012, 1220, 1350, 2189, 2593
FaqI GGGAC 3 cut(s) 631, 1227, 1954
FatI CATG 8 cut(s) 660, 712, 741, 1008, 1216, 1346, 2185, 2589
FauI CCCGC 1 cut(s) 416
FauNDI CATATG 1 cut(s) 2077
FbaI TGATCA 2 cut(s) 177, 2170
FblI GTMKAC 1 cut(s) 2133
Fnu4HI GCNGC 6 cut(s) 412, 1638, 1857, 2051, 2241, 2385
FriOI GRGCYC 2 cut(s) 1836, 2411
Fsp4HI GCNGC 6 cut(s) 412, 1638, 1857, 2051, 2241, 2385
FspBI CTAG 6 cut(s) 969, 1310, 1388, 1581, 1775, 1877
GluI GCNGC 6 cut(s) 412, 1638, 1857, 2051, 2241, 2385
GsuI CTGGAG 2 cut(s) 1647, 2319
HapII CCGG 8 cut(s) 515, 681, 827, 1419, 1454, 1891, 2139, 2664
HgaI GACGC 4 cut(s) 446, 832, 1418, 2168
Hin1I GRCGYC 2 cut(s) 438, 824
Hin1II CATG 8 cut(s) 664, 716, 745, 1012, 1220, 1350, 2189, 2593
HincII GTYRAC 3 cut(s) 799, 1237, 2515
HindII GTYRAC 3 cut(s) 799, 1237, 2515
HindIII AAGCTT 1 cut(s) 221
HpaI GTTAAC 1 cut(s) 1237
HpaII CCGG 8 cut(s) 515, 681, 827, 1419, 1454, 1891, 2139, 2664
Hpy166II GTNNAC 7 cut(s) 799, 1237, 1268, 1611, 2134, 2399, 2515
Hpy188I TCNGA 8 cut(s) 19, 585, 1163, 1434, 1697, 1704, 1810, 2247
Hpy8I GTNNAC 7 cut(s) 799, 1237, 1268, 1611, 2134, 2399, 2515
Hpy99I CGWCG 3 cut(s) 440, 761, 826
HpyAV CCTTC 9 cut(s) 317, 574, 977, 1094, 1162, 1864, 2189, 2479, 2495
HpyCH4III ACNGT 4 cut(s) 403, 678, 1450, 2573
HpyCH4IV ACGT 2 cut(s) 666, 1192
HpyF10VI GCNNNNNNNGC 3 cut(s) 959, 1862, 2292
HpyF3I CTNAG 3 cut(s) 72, 254, 2645
HpySE526I ACGT 2 cut(s) 666, 1192
Hsp92I GRCGYC 2 cut(s) 438, 824
Hsp92II CATG 8 cut(s) 664, 716, 745, 1012, 1220, 1350, 2189, 2593
KflI GGGWCCC 2 cut(s) 645, 1941
Kpn2I TCCGGA 2 cut(s) 1418, 2138
Ksp22I TGATCA 2 cut(s) 177, 2170
KspAI GTTAAC 1 cut(s) 1237
Lsp1109I GCAGC 5 cut(s) 1649, 1843, 2062, 2252, 2396
LweI GCATC 6 cut(s) 867, 981, 1003, 1053, 1466, 1868
MaeI CTAG 6 cut(s) 969, 1310, 1388, 1581, 1775, 1877
MaeII ACGT 2 cut(s) 666, 1192
MflI RGATCY 1 cut(s) 1767
MhlI GDGCHC 3 cut(s) 552, 1836, 2411
MlsI TGGCCA 1 cut(s) 1098
MluNI TGGCCA 1 cut(s) 1098
MlyI GAGTC 6 cut(s) 134, 266, 580, 1431, 1683, 2129
MmeI TCCRAC 6 cut(s) 480, 1457, 1690, 2136, 2460, 2619
Mox20I TGGCCA 1 cut(s) 1098
Mph1103I ATGCAT 1 cut(s) 2077
MroI TCCGGA 2 cut(s) 1418, 2138
MscI TGGCCA 1 cut(s) 1098
MseI TTAA 6 cut(s) 1055, 1236, 1440, 1544, 2523, 2680
MslI CAYNNNNRTG 4 cut(s) 665, 1092, 1916, 2178
Msp20I TGGCCA 1 cut(s) 1098
MspI CCGG 8 cut(s) 515, 681, 827, 1419, 1454, 1891, 2139, 2664
MspR9I CCNGG 6 cut(s) 515, 1125, 1211, 1227, 1538, 1671
Mva1269I GAATGC 1 cut(s) 2255
MvaI CCWGG 5 cut(s) 1125, 1211, 1227, 1538, 1671
MvnI CGCG 1 cut(s) 2193
MwoI GCNNNNNNNGC 3 cut(s) 959, 1862, 2292
NciI CCSGG 1 cut(s) 515
NdeI CATATG 1 cut(s) 2077
NheI GCTAGC 1 cut(s) 968
NlaIII CATG 8 cut(s) 664, 716, 745, 1012, 1220, 1350, 2189, 2593
NlaIV GGNNCC 8 cut(s) 646, 647, 706, 1122, 1769, 1780, 1942, 1943
NmuCI GTSAC 6 cut(s) 403, 672, 1552, 1649, 2037, 2657
NsiI ATGCAT 1 cut(s) 2077
NspI RCATGY 2 cut(s) 1220, 2189
NspV TTCGAA 2 cut(s) 262, 2259
PaeR7I CTCGAG 2 cut(s) 274, 2116
PceI AGGCCT 3 cut(s) 1881, 1957, 2225
PciI ACATGT 2 cut(s) 1216, 2185
PctI GAATGC 1 cut(s) 2255
PfeI GAWTC 6 cut(s) 140, 897, 1415, 1520, 1728, 1847
PfoI TCCNGGA 1 cut(s) 513
PinAI ACCGGT 2 cut(s) 1890, 2663
PkrI GCNGC 6 cut(s) 413, 1639, 1858, 2052, 2242, 2386
PleI GAGTC 6 cut(s) 133, 266, 580, 1430, 1683, 2129
PpsI GAGTC 6 cut(s) 133, 266, 580, 1430, 1683, 2129
PpuMI RGGWCCY 2 cut(s) 645, 1941
PscI ACATGT 2 cut(s) 1216, 2185
PshBI ATTAAT 1 cut(s) 1440
Psp124BI GAGCTC 2 cut(s) 1836, 2411
Psp5II RGGWCCY 2 cut(s) 645, 1941
Psp6I CCWGG 5 cut(s) 1123, 1209, 1225, 1536, 1669
PspEI GGTNACC 2 cut(s) 403, 672
PspGI CCWGG 5 cut(s) 1123, 1209, 1225, 1536, 1669
PspN4I GGNNCC 8 cut(s) 646, 647, 706, 1122, 1769, 1780, 1942, 1943
PspPI GGNCC 7 cut(s) 200, 484, 645, 818, 1120, 1779, 1941
PspPPI RGGWCCY 2 cut(s) 645, 1941
PspXI VCTCGAGB 1 cut(s) 274
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 1856
PsuI RGATCY 1 cut(s) 1767
RsaI GTAC 6 cut(s) 491, 536, 1254, 1610, 2184, 2400
RsaNI GTAC 6 cut(s) 490, 535, 1253, 1609, 2183, 2399
RseI CAYNNNNRTG 4 cut(s) 665, 1092, 1916, 2178
SacI GAGCTC 2 cut(s) 1836, 2411
SaqAI TTAA 6 cut(s) 1055, 1236, 1440, 1544, 2523, 2680
SatI GCNGC 6 cut(s) 412, 1638, 1857, 2051, 2241, 2385
Sau96I GGNCC 7 cut(s) 200, 484, 645, 818, 1120, 1779, 1941
SchI GAGTC 6 cut(s) 134, 266, 580, 1431, 1683, 2129
ScrFI CCNGG 6 cut(s) 515, 1125, 1211, 1227, 1538, 1671
SduI GDGCHC 3 cut(s) 552, 1836, 2411
SfaNI GCATC 6 cut(s) 867, 981, 1003, 1053, 1466, 1868
SfcI CTRYAG 3 cut(s) 699, 1756, 2619
Sfr274I CTCGAG 2 cut(s) 274, 2116
SfuI TTCGAA 2 cut(s) 262, 2259
SgrAI CRCCGGYG 1 cut(s) 2663
SinI GGWCC 5 cut(s) 200, 484, 645, 1120, 1941
SlaI CTCGAG 2 cut(s) 274, 2116
SmiMI CAYNNNNRTG 4 cut(s) 665, 1092, 1916, 2178
SmlI CTYRAG 3 cut(s) 274, 938, 2116
SmoI CTYRAG 3 cut(s) 274, 938, 2116
SseBI AGGCCT 3 cut(s) 1881, 1957, 2225
SsiI CCGC 7 cut(s) 409, 412, 789, 1825, 2165, 2191, 2286
SspMI CTAG 6 cut(s) 969, 1310, 1388, 1581, 1775, 1877
SstI GAGCTC 2 cut(s) 1836, 2411
StuI AGGCCT 3 cut(s) 1881, 1957, 2225
StyD4I CCNGG 6 cut(s) 513, 1123, 1209, 1225, 1536, 1669
TaaI ACNGT 4 cut(s) 403, 678, 1450, 2573
TaiI ACGT 2 cut(s) 669, 1195
TaqI TCGA 7 cut(s) 262, 275, 344, 435, 2117, 2259, 2331
TatI WGTACW 5 cut(s) 489, 1252, 1608, 2182, 2398
TauI GCSGC 1 cut(s) 414
TfiI GAWTC 6 cut(s) 140, 897, 1415, 1520, 1728, 1847
Tru1I TTAA 6 cut(s) 1055, 1236, 1440, 1544, 2523, 2680
Tru9I TTAA 6 cut(s) 1055, 1236, 1440, 1544, 2523, 2680
TscAI CASTG 5 cut(s) 1561, 1625, 2130, 2302, 2611
TseFI GTSAC 6 cut(s) 403, 672, 1552, 1649, 2037, 2657
TseI GCWGC 5 cut(s) 1637, 1856, 2050, 2240, 2384
Tsp45I GTSAC 6 cut(s) 403, 672, 1552, 1649, 2037, 2657
TspDTI ATGAA 8 cut(s) 20, 55, 252, 702, 847, 1352, 1487, 2461
TspGWI ACGGA 3 cut(s) 866, 1874, 2406
TspRI CASTG 5 cut(s) 1561, 1625, 2130, 2302, 2611
VpaK11BI GGWCC 5 cut(s) 200, 484, 645, 1120, 1941
VspI ATTAAT 1 cut(s) 1440
XagI CCTNNNNNAGG 2 cut(s) 941, 981
XapI RAATTY 5 cut(s) 258, 1681, 2062, 2369, 2533
XceI RCATGY 2 cut(s) 1220, 2189
XhoI CTCGAG 2 cut(s) 274, 2116
XmiI GTMKAC 1 cut(s) 2133
XspI CTAG 6 cut(s) 969, 1310, 1388, 1581, 1775, 1877
Zsp2I ATGCAT 1 cut(s) 2077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.