Rroxscaffold_1G00059120

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000882
Physical Location & Seq
Reverse (-)
81114914 .. 81116119
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059120.1

Sequence Viewer

Length: 990 bp
ATGGTTGGAGCTGATATATGTGGATTCACTTTGAATACTACTGAAGAACTCTGCAGGCGATGGATCCAGCTAGGGGCCTTTTACCCGTTTGAAAGAGATCATTCAGATATATTTACAATCCGCCAAGAGCTCTATGTTTGGGATTCAGTTGCTGCATCAGCAAGGAAGGTGCTAGGCCTCCGTTACCGGTTGCTCCCCTTGTTCTACACATCAATGTATCAGGCACACAAAAAAGGGACCCCCATTGCAAGGCCCCTTTTCTTCTCTTTTCCTGAAGATACCAATACATACAACATCAATTCACAGTTCTTGATTGGTAAAGGTGTGATAGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTTATGCATATTTTCCTGCTGGAAACTGGTTTGATCTCTTCAACTACTCGAGATCAGTGAGTGTAGACTCCGGAGAGAATGTTATGTTGGACGCACCGCCTGATCATATTAATGTACATGTCCGCGAAGGAAACATTTTGGCCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCTCGGAAGACTGCATTCGAACTTTTGGTGGTCATTAGTAGATGCGGAGAGAGCACCGGAGAAGTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTAGTGAAATTCTATTGTGGAGCAGCAAATGGAAGTGTATTTTTGAGCTCAAAAGTTGGAAATGGAGGATTTGCTTTGAGCCAAAAATGGATCATTGATAAAGTTACCTTCATTGGGTTGGAAAAAGTTGATGGATTAGAAGGGTTTGCAGTCAACTTTATTAAGGGAACAAATTTGAAAGGAATGTGGGATGTCAATGCCAAATTTCACGGTGATAAGCGTTTTGTCATGGTAAAAATATCCAGCGTATCCATTCCTATAGGAAAAGAGTTTGAGTTGGAGCTGAGCCTAAAGCGTGACACCGGTGAAAACACTCTTGAAGGGAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

329

Amino Acids

36.5

Weight (kDa)

5.51

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_31_2nd PF01055 1 - 70 2.1e-25 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 79 - 168 3.8e-25 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 429
AccII CGCG 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 434
AciI CCGC 4 cut(s) 121, 461, 487, 582
AclWI GGATC 3 cut(s) 58, 71, 755
AcsI RAATTY 4 cut(s) 358, 665, 829, 860
AcuI CTGAAG 2 cut(s) 63, 294
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 3 cut(s) 73, 249, 771
AflIII ACRYGT 1 cut(s) 481
AgeI ACCGGT 2 cut(s) 186, 959
AgsI TTSAA 5 cut(s) 34, 92, 406, 835, 977
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 6 cut(s) 11, 70, 130, 539, 705, 940
AluI AGCT 6 cut(s) 11, 70, 130, 539, 705, 940
Alw21I GWGCWC 3 cut(s) 132, 593, 707
AlwI GGATC 3 cut(s) 58, 71, 755
AlwNI CAGNNNCTG 1 cut(s) 152
Ama87I CYCGRG 1 cut(s) 412
Aor13HI TCCGGA 1 cut(s) 434
AoxI GGCC 5 cut(s) 75, 175, 251, 504, 519
ApeKI GCWGC 4 cut(s) 152, 346, 536, 680
ApoI RAATTY 4 cut(s) 358, 665, 829, 860
AseI ATTAAT 1 cut(s) 474
AsiGI ACCGGT 2 cut(s) 186, 959
AspS9I GGNCC 3 cut(s) 75, 237, 252
AsuHPI GGTGA 3 cut(s) 327, 881, 974
AsuII TTCGAA 1 cut(s) 555
AvaI CYCGRG 1 cut(s) 412
AvaII GGWCC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 63
BanII GRGCYC 2 cut(s) 132, 707
BbsI GAAGAC 1 cut(s) 551
Bbv12I GWGCWC 3 cut(s) 132, 593, 707
BbvI GCAGC 4 cut(s) 139, 358, 548, 692
BccI CCATC 3 cut(s) 54, 608, 782
BciVI GTATCC 1 cut(s) 916
BclI TGATCA 1 cut(s) 466
BfaI CTAG 2 cut(s) 71, 173
BfmI CTRYAG 2 cut(s) 52, 915
BfuI GTATCC 1 cut(s) 916
BisI GCNGC 4 cut(s) 153, 347, 537, 681
BlpI GCTNAGC 1 cut(s) 941
BlsI GCNGC 4 cut(s) 154, 348, 538, 682
Bme18I GGWCC 1 cut(s) 237
BmeT110I CYCGRG 1 cut(s) 412
BmgT120I GGNCC 3 cut(s) 75, 237, 252
BmiI GGNNCC 5 cut(s) 65, 76, 238, 239, 254
BmsI GCATC 2 cut(s) 164, 569
BpiI GAAGAC 1 cut(s) 551
Bpu1102I GCTNAGC 1 cut(s) 941
Bpu14I TTCGAA 1 cut(s) 555
BsaWI WCCGGW 4 cut(s) 186, 434, 593, 959
BsaXI ACNNNNNCTCC 4 cut(s) 429, 459, 609, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 249, 771
Bse118I RCCGGY 2 cut(s) 186, 959
Bse1I ACTGG 1 cut(s) 395
Bse3DI GCAATG 1 cut(s) 243
BseAI TCCGGA 1 cut(s) 434
BseGI GGATG 2 cut(s) 616, 853
BseLI CCNNNNNNNGG 3 cut(s) 73, 249, 771
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 932
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 651
BseXI GCAGC 4 cut(s) 139, 358, 548, 692
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 5 cut(s) 77, 177, 253, 506, 521
BshTI ACCGGT 2 cut(s) 186, 959
BsiHKAI GWGCWC 3 cut(s) 132, 593, 707
BsiHKCI CYCGRG 1 cut(s) 412
BsiSI CCGG 4 cut(s) 187, 435, 594, 960
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 3 cut(s) 73, 249, 771
BsmFI GGGAC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 551
BsnI GGCC 5 cut(s) 77, 177, 253, 506, 521
BsoBI CYCGRG 1 cut(s) 412
Bsp119I TTCGAA 1 cut(s) 555
Bsp1286I GDGCHC 3 cut(s) 132, 593, 707
Bsp13I TCCGGA 1 cut(s) 434
Bsp1407I TGTACA 1 cut(s) 478
Bsp143I GATC 6 cut(s) 63, 97, 397, 416, 466, 747
Bsp1720I GCTNAGC 1 cut(s) 941
BspACI CCGC 4 cut(s) 121, 461, 487, 582
BspANI GGCC 5 cut(s) 77, 177, 253, 506, 521
BspCNI CTCAG 1 cut(s) 933
BspEI TCCGGA 1 cut(s) 434
BspFNI CGCG 1 cut(s) 489
BspLI GGNNCC 5 cut(s) 65, 76, 238, 239, 254
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 3 cut(s) 58, 71, 755
BspT104I TTCGAA 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 243
BsrFI RCCGGY 2 cut(s) 186, 959
BsrGI TGTACA 1 cut(s) 478
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 2 cut(s) 186, 959
BssMI GATC 6 cut(s) 63, 97, 397, 416, 466, 747
Bst4CI ACNGT 2 cut(s) 306, 869
Bst6I CTCTTC 1 cut(s) 407
BstAUI TGTACA 1 cut(s) 478
BstBI TTCGAA 1 cut(s) 555
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 1 cut(s) 941
BstF5I GGATG 2 cut(s) 616, 853
BstFNI CGCG 1 cut(s) 489
BstKTI GATC 6 cut(s) 66, 100, 400, 419, 469, 750
BstMBI GATC 6 cut(s) 63, 97, 397, 416, 466, 747
BstMWI GCNNNNNNNGC 2 cut(s) 158, 588
BstNSI RCATGY 1 cut(s) 485
BstSFI CTRYAG 2 cut(s) 52, 915
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 4 cut(s) 139, 358, 548, 692
BstV2I GAAGAC 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
BsuI GTATCC 1 cut(s) 916
BsuRI GGCC 5 cut(s) 77, 177, 253, 506, 521
BtgZI GCGATG 1 cut(s) 73
BtsCI GGATG 2 cut(s) 616, 853
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 1 cut(s) 56
CaiI CAGNNNCTG 1 cut(s) 152
Cfr10I RCCGGY 2 cut(s) 186, 959
Cfr13I GGNCC 3 cut(s) 75, 237, 252
CseI GACGC 1 cut(s) 464
Csp6I GTAC 1 cut(s) 479
CspAI ACCGGT 2 cut(s) 186, 959
CviAII CATG 2 cut(s) 482, 886
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 1 cut(s) 941
DpnI GATC 6 cut(s) 65, 99, 399, 418, 468, 749
DpnII GATC 6 cut(s) 63, 97, 397, 416, 466, 747
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
EciI GGCGGA 1 cut(s) 110
Ecl136II GAGCTC 2 cut(s) 130, 705
Eco147I AGGCCT 2 cut(s) 177, 521
Eco24I GRGCYC 2 cut(s) 132, 707
Eco47I GGWCC 1 cut(s) 237
Eco53kI GAGCTC 2 cut(s) 130, 705
Eco57I CTGAAG 2 cut(s) 63, 294
Eco88I CYCGRG 1 cut(s) 412
EcoICRI GAGCTC 2 cut(s) 130, 705
EcoO109I RGGNCCY 3 cut(s) 75, 237, 252
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 2 cut(s) 132, 707
FaeI CATG 2 cut(s) 485, 889
FaqI GGGAC 1 cut(s) 250
FatI CATG 2 cut(s) 481, 885
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 4 cut(s) 153, 347, 537, 681
FokI GGATG 2 cut(s) 623, 860
FriOI GRGCYC 2 cut(s) 132, 707
Fsp4HI GCNGC 4 cut(s) 153, 347, 537, 681
FspBI CTAG 2 cut(s) 71, 173
GluI GCNGC 4 cut(s) 153, 347, 537, 681
HaeIII GGCC 5 cut(s) 77, 177, 253, 506, 521
HapII CCGG 4 cut(s) 187, 435, 594, 960
HgaI GACGC 1 cut(s) 464
Hin1II CATG 2 cut(s) 485, 889
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HinfI GANTC 3 cut(s) 24, 143, 431
HpaII CCGG 4 cut(s) 187, 435, 594, 960
HphI GGTGA 3 cut(s) 327, 881, 974
Hpy166II GTNNAC 2 cut(s) 430, 811
Hpy188I TCNGA 2 cut(s) 106, 543
Hpy188III TCNNGA 5 cut(s) 272, 310, 414, 435, 974
Hpy8I GTNNAC 2 cut(s) 430, 811
HpyAV CCTTC 5 cut(s) 160, 485, 775, 791, 971
HpyCH4III ACNGT 2 cut(s) 306, 869
HpyCH4V TGCA 8 cut(s) 54, 155, 248, 346, 371, 511, 551, 806
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 588
HpyF3I CTNAG 1 cut(s) 941
Hsp92II CATG 2 cut(s) 485, 889
KflI GGGWCCC 1 cut(s) 237
Kpn2I TCCGGA 1 cut(s) 434
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 6 cut(s) 63, 97, 397, 416, 466, 747
LmnI GCTCC 5 cut(s) 8, 198, 353, 677, 937
Lsp1109I GCAGC 4 cut(s) 139, 358, 548, 692
LweI GCATC 2 cut(s) 164, 569
MaeI CTAG 2 cut(s) 71, 173
MaeIII GTNAC 4 cut(s) 182, 333, 760, 953
MalI GATC 6 cut(s) 65, 99, 399, 418, 468, 749
MboI GATC 6 cut(s) 63, 97, 397, 416, 466, 747
MboII GAAGA 5 cut(s) 56, 253, 287, 394, 556
MflI RGATCY 1 cut(s) 63
MhlI GDGCHC 3 cut(s) 132, 593, 707
MluCI AATT 6 cut(s) 298, 358, 665, 829, 860, 985
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 4 cut(s) 432, 694, 756, 915
MnlI CCTC 7 cut(s) 188, 511, 613, 629, 634, 638, 716
Mph1103I ATGCAT 1 cut(s) 373
MroI TCCGGA 1 cut(s) 434
MseI TTAA 2 cut(s) 474, 819
MslI CAYNNNNRTG 2 cut(s) 212, 474
MspI CCGG 4 cut(s) 187, 435, 594, 960
Mva1269I GAATGC 1 cut(s) 551
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 2 cut(s) 158, 588
NdeII GATC 6 cut(s) 63, 97, 397, 416, 466, 747
NlaIII CATG 2 cut(s) 485, 889
NlaIV GGNNCC 5 cut(s) 65, 76, 238, 239, 254
NmuCI GTSAC 2 cut(s) 333, 953
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 1 cut(s) 485
NspV TTCGAA 1 cut(s) 555
PaeR7I CTCGAG 1 cut(s) 412
PceI AGGCCT 2 cut(s) 177, 521
PciI ACATGT 1 cut(s) 481
PctI GAATGC 1 cut(s) 551
PfeI GAWTC 2 cut(s) 24, 143
PinAI ACCGGT 2 cut(s) 186, 959
PkrI GCNGC 4 cut(s) 154, 348, 538, 682
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
PpuMI RGGWCCY 1 cut(s) 237
PscI ACATGT 1 cut(s) 481
PshBI ATTAAT 1 cut(s) 474
Psp124BI GAGCTC 2 cut(s) 132, 707
Psp5II RGGWCCY 1 cut(s) 237
PspN4I GGNNCC 5 cut(s) 65, 76, 238, 239, 254
PspPI GGNCC 3 cut(s) 75, 237, 252
PspPPI RGGWCCY 1 cut(s) 237
PstI CTGCAG 1 cut(s) 56
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 63
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 2 cut(s) 212, 474
SacI GAGCTC 2 cut(s) 132, 707
SaqAI TTAA 2 cut(s) 474, 819
SatI GCNGC 4 cut(s) 153, 347, 537, 681
Sau3AI GATC 6 cut(s) 63, 97, 397, 416, 466, 747
Sau96I GGNCC 3 cut(s) 75, 237, 252
SchI GAGTC 1 cut(s) 425
SduI GDGCHC 3 cut(s) 132, 593, 707
SfaNI GCATC 2 cut(s) 164, 569
SfcI CTRYAG 2 cut(s) 52, 915
Sfr274I CTCGAG 1 cut(s) 412
SfuI TTCGAA 1 cut(s) 555
SgrAI CRCCGGYG 1 cut(s) 959
SinI GGWCC 1 cut(s) 237
SlaI CTCGAG 1 cut(s) 412
SmiMI CAYNNNNRTG 2 cut(s) 212, 474
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
Sse9I AATT 6 cut(s) 298, 358, 665, 829, 860, 985
SseBI AGGCCT 2 cut(s) 177, 521
SsiI CCGC 4 cut(s) 121, 461, 487, 582
SspMI CTAG 2 cut(s) 71, 173
SstI GAGCTC 2 cut(s) 132, 707
StuI AGGCCT 2 cut(s) 177, 521
TaaI ACNGT 2 cut(s) 306, 869
TaqI TCGA 3 cut(s) 413, 555, 627
TasI AATT 6 cut(s) 298, 358, 665, 829, 860, 985
TatI WGTACW 1 cut(s) 478
TfiI GAWTC 2 cut(s) 24, 143
Tru1I TTAA 2 cut(s) 474, 819
Tru9I TTAA 2 cut(s) 474, 819
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 2 cut(s) 333, 953
TseI GCWGC 4 cut(s) 152, 346, 536, 680
Tsp45I GTSAC 2 cut(s) 333, 953
TspDTI ATGAA 1 cut(s) 757
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 237
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 4 cut(s) 358, 665, 829, 860
XceI RCATGY 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 412
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 2 cut(s) 71, 173
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.