pycom10g20100

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000882
Physical Location & Seq
Reverse (-)
23073116 .. 23076079
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20100.3

Sequence Viewer

Length: 2382 bp
ATGATCCCCCGCCAAACCAAAACCCCCACCCCCCACCACCACTGCATCTCCCACGACCTTTTCTTCACTCTCCGCAACACCACCCCATTCGGCTTCACCGTCGCCCGCTGCTCCTCCAAGGACGTCATCTTCGACACCTCCCCCCACTCGTCAGACCCCAACACCTTCTTGGTCTTCAAGGACCAATACATCCAACTCTCCTCCTCTCTCCCCCATAACAGATCCTCCATTTTCGGCCTCGGCGAGCACACCAAGTCCTCCTTCACGATCATTCCCAATCAGACATTGACTCTCTGGAATGCCGACATTGGTAGCGTCAATCCCGACGTCAACCTTTACGGGTCCCACCCCTTCTACCTGGACGTCAGGTCGGCGTCTCAAGCTGGCGGCAAGACCGGTGCTGGAACATCTCATGGAGTGCTGCTGCTGAATAGCAATGGCATGGACATAACATACAGCGGTGATAGGATTACTTATAAGGTCATCGGTGGAATTGTGGACTTGTACTTCTTTGCCGGGCCGACACCTGAGTCGGTAGTGGAGCAGTATACAGAACTAATCGGCCGCCCTGCCCCTATGCCCTACTGGTCTTTTGGATTCCACCAGTGTCGATGGGGTTACAAGAATGTTTCGGATCTTGAGGGCGTGGTTGCTGGTTATACAAAAGCCGGCATCCCTCTTGAAGTTATGTGGACGGACATCGATTACATGGATGCTTACAAGGACTTCACTCTCGATCCCGTCAACTTTCCATTGGATAAGATGAAGAAATTTGTAAGCACTCTTCACCAAAATGGTCAGAAATATGTCCTCATCTTGGACCCTGGTATTAGTAATAATGACACCTATGAAACCTACATCAGAGGGAAGGAAGCAGATATTTTCATCAAACGCGACGGAATTCCTTACCTGGGAAATGTATGGCCCGGCGATGTGTACTACCCAGACTTTGCACATCCAGAAAGTGAAAAATATTGGGCTAATGAGATACAAATATTTCAAAAGGCTCTGCCTTTTGACGGTCTTTGGCTCGATATGAATGAGCTATCCAATTTCAGAACCTCCCCTCCCACTCCATCTTCTACACTTGATGACCCTCCTTACAAGGTTAACAATTCTGGAGTCCTGCGTCCGATCGATAACAATACTGTTCCGGCATCAGCGCTGCACTTTGGTAACATTACAGAATATAATGCACATAACTTGTATGGACTCTTGGAAGCCAAGGCAACAAGCAACGCCTTAATCAATGTAACTGGTAAAAGACCGTTTATACTTTCTAGATCGACTTTTGTAAGCTCCGGTAAGTACACAGCACACTGGACTGGTGACAATTCTGCACGATGGAGTGATCTGGCATATACAATTCCTGGCATTTTAAATTTTGGACTTTTCGGAGTTCCAATGGTTGGAGCTGACATATGTGGATTCTCTGGAGATACTACCGAAGAACTCTGCCGGCGATGGATTCAGCTTGGGGCCTTTTACCCCTTTTCAAGAGATCACTCCGAAAAGTTTACAATCCGTCAAGAGCTCTATCTATGGGACTCAGTGGCAGCAACATCCAGGAAGGTGCTTGGGCTGCGCTACCGGTTGCTCCCCTTATTTTACACATCAATGTATGAGGCACACAAAAAGGGAACCCCAATTGCAAGACCCCTTTTCTTTTCATTTCCTCAAGATATCACAACTTACGGGATCAATACGCAGTTTCTAATTGGTCGAGGTGTAATGGTCTCACCTGTGCTGAAACCGGGAGTCAATTCAGTTGATGCATATTTTCCTGCTGGAAACTGGTTTGATCTCTTCAACTACTCAAAATCTGTAAGTGTAAGTTCAGGACAGCACGTTAGGTTGGATGCGCCGCCTGATCATATCAATGTGCATGTTTGTGAAGGAAATATTTTGGCATTGCAAGGAGAGGCCTTGACAACACAAGCAGCCCGAAAGACTGCTTTCGAGCTTTTGGTGGTCGTTAGCAGTAACAGACAAAGTACAGGAGAAGTTTTCGTGGACGACGGAGAGGAAATTGAAATGGGAGGAGAGGGAGGCAAGTGGAGTTTAGTGAGATTTTATAGTGGAACAGAAAATGGAACTGTATTTGTAAGCTCAAAGGTTGTAAATGGAGGATTCGCTTTAAGTGAAAAATGGATCATTGATAAAGTTACCTTCATTGGGTTGGAAAAGGTTGGTCATGGATTGGAAGGGTGTGCATTCAACATTACTAAGGGAGCAAATTTGAAAGCAAACTCGGATATCAGAGCCAGCTTTGACAGTAACAAGCAGTTTGTCACAGCAGTGATTTCCGACATATCGATTCTTATAGGATCAGAGTTTAAGTTGGAGCTGAAGCCGAAGCACGCCACTGAAAGCACTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

794

Amino Acids

88.03

Weight (kDa)

6.01

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AasI GACNNNNNNGTC 1 cut(s) 529
AatII GACGTC 3 cut(s) 126, 330, 366
AccB7I CCANNNNNTGG 1 cut(s) 1409
AccI GTMKAC 1 cut(s) 548
AccII CGCG 1 cut(s) 894
AciI CCGC 7 cut(s) 10, 73, 106, 387, 459, 565, 1865
AclWI GGATC 6 cut(s) 216, 642, 731, 1706, 2159, 2335
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 4 cut(s) 770, 900, 1381, 2236
AcuI CTGAAG 1 cut(s) 2369
AcyI GRCGYC 4 cut(s) 123, 327, 363, 374
AfaI GTAC 4 cut(s) 506, 938, 1310, 1996
AfeI AGCGCT 1 cut(s) 1164
AfiI CCNNNNNNNGG 5 cut(s) 817, 911, 1019, 1409, 2175
AgeI ACCGGT 2 cut(s) 395, 1590
AgsI TTSAA 8 cut(s) 178, 683, 1001, 1497, 1810, 2033, 2218, 2242
AhdI GACNNNNNGTC 1 cut(s) 367
AjnI CCWGG 5 cut(s) 357, 823, 909, 1369, 1565
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AleI CACNNNNGTG 1 cut(s) 2297
AloI GAACNNNNNNTCC 2 cut(s) 1051, 1083
Alw21I GWGCWC 2 cut(s) 249, 1536
Alw26I GTCTC 2 cut(s) 381, 1741
AlwI GGATC 6 cut(s) 216, 642, 731, 1706, 2159, 2335
Aor51HI AGCGCT 1 cut(s) 1164
AoxI GGCC 6 cut(s) 235, 518, 562, 923, 1479, 1923
ApeKI GCWGC 7 cut(s) 108, 421, 424, 1165, 1556, 1582, 1940
ApoI RAATTY 4 cut(s) 770, 900, 1381, 2236
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AsiGI ACCGGT 2 cut(s) 395, 1590
AspLEI GCGC 3 cut(s) 1165, 1587, 1864
AspS9I GGNCC 6 cut(s) 181, 342, 518, 820, 924, 1479
AsuC2I CCSGG 3 cut(s) 517, 927, 1755
AsuHPI GGTGA 5 cut(s) 88, 473, 779, 1340, 1731
AvaII GGWCC 3 cut(s) 181, 342, 820
BanII GRGCYC 1 cut(s) 1536
BarI GAAGNNNNNNTAC 2 cut(s) 768, 800
BbsI GAAGAC 1 cut(s) 166
Bbv12I GWGCWC 2 cut(s) 249, 1536
BbvI GCAGC 7 cut(s) 95, 408, 411, 1152, 1568, 1569, 1952
BccI CCATC 4 cut(s) 606, 1084, 1338, 1458
BciT130I CCWGG 5 cut(s) 359, 825, 911, 1371, 1567
BclI TGATCA 1 cut(s) 1870
BcnI CCSGG 3 cut(s) 517, 927, 1755
BcoDI GTCTC 2 cut(s) 381, 1741
BfaI CTAG 1 cut(s) 1281
BfoI RGCGCY 1 cut(s) 1166
Bme1390I CCNGG 8 cut(s) 359, 517, 825, 911, 927, 1371, 1567, 1755
Bme18I GGWCC 3 cut(s) 181, 342, 820
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgT120I GGNCC 6 cut(s) 181, 342, 518, 820, 924, 1479
BmiI GGNNCC 5 cut(s) 343, 344, 822, 1480, 1642
BmrFI CCNGG 8 cut(s) 359, 517, 825, 911, 927, 1371, 1567, 1755
BmsI GCATC 6 cut(s) 54, 681, 703, 1166, 1762, 1849
BpiI GAAGAC 1 cut(s) 166
BpmI CTGGAG 2 cut(s) 1140, 1455
BpuEI CTTGAG 3 cut(s) 363, 659, 1662
BpuMI CCSGG 3 cut(s) 517, 927, 1755
Bsa29I ATCGAT 3 cut(s) 702, 1137, 2315
BsaHI GRCGYC 4 cut(s) 123, 327, 363, 374
BsaI GGTCTC 1 cut(s) 1741
BsaJI CCNNGG 5 cut(s) 117, 238, 823, 910, 1224
BsaWI WCCGGW 3 cut(s) 395, 1301, 1590
BsaXI ACNNNNNCTCC 6 cut(s) 32, 62, 209, 239, 1051, 1081
Bsc4I CCNNNNNNNGG 5 cut(s) 817, 911, 1019, 1409, 2175
Bse118I RCCGGY 4 cut(s) 395, 668, 1458, 1590
Bse1I ACTGG 6 cut(s) 590, 604, 1261, 1325, 1330, 1799
Bse3DI GCAATG 2 cut(s) 442, 1910
BseBI CCWGG 5 cut(s) 359, 825, 911, 1371, 1567
BseCI ATCGAT 3 cut(s) 702, 1137, 2315
BseDI CCNNGG 5 cut(s) 117, 238, 823, 910, 1224
BseGI GGATG 6 cut(s) 189, 672, 718, 955, 1562, 1864
BseLI CCNNNNNNNGG 5 cut(s) 817, 911, 1019, 1409, 2175
BseMI GCAATG 2 cut(s) 442, 1910
BseMII CTCAG 2 cut(s) 519, 1563
BseNI ACTGG 6 cut(s) 590, 604, 1261, 1325, 1330, 1799
BseRI GAGGAG 4 cut(s) 103, 190, 193, 2055
BseX3I CGGCCG 1 cut(s) 562
BseXI GCAGC 7 cut(s) 95, 408, 411, 1152, 1568, 1569, 1952
BsgI GTGCAG 2 cut(s) 1151, 1323
Bsh1236I CGCG 1 cut(s) 894
Bsh1285I CGRYCG 2 cut(s) 565, 1137
BshFI GGCC 6 cut(s) 237, 520, 564, 925, 1481, 1925
BshTI ACCGGT 2 cut(s) 395, 1590
BshVI ATCGAT 3 cut(s) 702, 1137, 2315
BsiEI CGRYCG 2 cut(s) 565, 1137
BsiHKAI GWGCWC 2 cut(s) 249, 1536
BsiSI CCGG 9 cut(s) 396, 516, 669, 927, 1154, 1302, 1459, 1591, 1754
BslFI GGGAC 2 cut(s) 328, 1559
BslI CCNNNNNNNGG 5 cut(s) 817, 911, 1019, 1409, 2175
BsmAI GTCTC 2 cut(s) 381, 1741
BsmBI CGTCTC 1 cut(s) 381
BsmFI GGGAC 2 cut(s) 328, 1559
BsmI GAATGC 2 cut(s) 304, 2213
BsnI GGCC 6 cut(s) 237, 520, 564, 925, 1481, 1925
Bso31I GGTCTC 1 cut(s) 1741
Bsp1286I GDGCHC 2 cut(s) 249, 1536
BspACI CCGC 7 cut(s) 10, 73, 106, 387, 459, 565, 1865
BspANI GGCC 6 cut(s) 237, 520, 564, 925, 1481, 1925
BspCNI CTCAG 2 cut(s) 520, 1562
BspDI ATCGAT 3 cut(s) 702, 1137, 2315
BspFNI CGCG 1 cut(s) 894
BspLI GGNNCC 5 cut(s) 343, 344, 822, 1480, 1642
BspPI GGATC 6 cut(s) 216, 642, 731, 1706, 2159, 2335
BspTNI GGTCTC 1 cut(s) 1741
BsrDI GCAATG 2 cut(s) 442, 1910
BsrFI RCCGGY 4 cut(s) 395, 668, 1458, 1590
BsrI ACTGG 6 cut(s) 590, 604, 1261, 1325, 1330, 1799
BssAI RCCGGY 4 cut(s) 395, 668, 1458, 1590
BssECI CCNNGG 5 cut(s) 117, 238, 823, 910, 1224
BssNAI GTATAC 1 cut(s) 549
BssNI GRCGYC 4 cut(s) 123, 327, 363, 374
BssT1I CCWWGG 2 cut(s) 117, 1224
Bst1107I GTATAC 1 cut(s) 549
Bst2UI CCWGG 5 cut(s) 359, 825, 911, 1371, 1567
Bst4CI ACNGT 6 cut(s) 100, 1022, 1150, 1269, 2098, 2276
Bst6I CTCTTC 2 cut(s) 789, 1811
BstACI GRCGYC 4 cut(s) 123, 327, 363, 374
BstC8I GCNNGC 7 cut(s) 106, 245, 385, 670, 1460, 2266, 2361
BstDEI CTNAG 3 cut(s) 528, 1549, 2226
BstF5I GGATG 6 cut(s) 189, 672, 718, 955, 1562, 1864
BstFNI CGCG 1 cut(s) 894
BstH2I RGCGCY 1 cut(s) 1166
BstHHI GCGC 3 cut(s) 1165, 1587, 1864
BstMAI GTCTC 2 cut(s) 381, 1741
BstMCI CGRYCG 2 cut(s) 565, 1137
BstMWI GCNNNNNNNGC 2 cut(s) 380, 1582
BstNI CCWGG 5 cut(s) 359, 825, 911, 1371, 1567
BstNSI RCATGY 1 cut(s) 1889
BstSCI CCNGG 8 cut(s) 357, 515, 823, 909, 925, 1369, 1565, 1753
BstUI CGCG 1 cut(s) 894
BstV1I GCAGC 7 cut(s) 95, 408, 411, 1152, 1568, 1569, 1952
BstV2I GAAGAC 1 cut(s) 166
BstX2I RGATCY 2 cut(s) 221, 634
BstYI RGATCY 2 cut(s) 221, 634
BstZ17I GTATAC 1 cut(s) 549
BstZI CGGCCG 1 cut(s) 562
Bsu15I ATCGAT 3 cut(s) 702, 1137, 2315
BsuRI GGCC 6 cut(s) 237, 520, 564, 925, 1481, 1925
BsuTUI ATCGAT 3 cut(s) 702, 1137, 2315
BtgZI GCGATG 2 cut(s) 945, 1477
BtsCI GGATG 6 cut(s) 189, 672, 718, 955, 1562, 1864
BtsI GCAGTG 2 cut(s) 40, 2304
BtsIMutI CAGTG 6 cut(s) 40, 611, 1318, 1557, 2304, 2364
Cac8I GCNNGC 7 cut(s) 106, 245, 385, 670, 1460, 2266, 2361
CfoI GCGC 3 cut(s) 1165, 1587, 1864
Cfr10I RCCGGY 4 cut(s) 395, 668, 1458, 1590
Cfr13I GGNCC 6 cut(s) 181, 342, 518, 820, 924, 1479
ClaI ATCGAT 3 cut(s) 702, 1137, 2315
CseI GACGC 3 cut(s) 304, 363, 1118
Csp6I GTAC 4 cut(s) 505, 937, 1309, 1995
CspAI ACCGGT 2 cut(s) 395, 1590
CviAII CATG 5 cut(s) 413, 442, 709, 1886, 2195
CviQI GTAC 4 cut(s) 505, 937, 1309, 1995
DdeI CTNAG 3 cut(s) 528, 1549, 2226
DraI TTTAAA 1 cut(s) 1380
DrdI GACNNNNNNGTC 1 cut(s) 529
DriI GACNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 562
EagI CGGCCG 1 cut(s) 562
Eam1104I CTCTTC 2 cut(s) 789, 1811
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 2 cut(s) 789, 1811
Ecl136II GAGCTC 1 cut(s) 1534
EclXI CGGCCG 1 cut(s) 562
Eco130I CCWWGG 2 cut(s) 117, 1224
Eco147I AGGCCT 1 cut(s) 1925
Eco24I GRGCYC 1 cut(s) 1536
Eco31I GGTCTC 1 cut(s) 1741
Eco32I GATATC 2 cut(s) 1684, 2257
Eco47I GGWCC 3 cut(s) 181, 342, 820
Eco47III AGCGCT 1 cut(s) 1164
Eco52I CGGCCG 1 cut(s) 562
Eco53kI GAGCTC 1 cut(s) 1534
Eco57I CTGAAG 1 cut(s) 2369
EcoICRI GAGCTC 1 cut(s) 1534
EcoO109I RGGNCCY 2 cut(s) 342, 1479
EcoRI GAATTC 1 cut(s) 900
EcoRII CCWGG 5 cut(s) 357, 823, 909, 1369, 1565
EcoRV GATATC 2 cut(s) 1684, 2257
EcoT14I CCWWGG 2 cut(s) 117, 1224
EcoT22I ATGCAT 1 cut(s) 1777
EcoT38I GRGCYC 1 cut(s) 1536
ErhI CCWWGG 2 cut(s) 117, 1224
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 5 cut(s) 416, 445, 712, 1889, 2198
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 2 cut(s) 328, 1559
FatI CATG 5 cut(s) 412, 441, 708, 1885, 2194
FauI CCCGC 2 cut(s) 17, 113
FauNDI CATATG 1 cut(s) 1421
FbaI TGATCA 1 cut(s) 1870
FblI GTMKAC 1 cut(s) 548
FokI GGATG 6 cut(s) 176, 659, 725, 942, 1549, 1871
FriOI GRGCYC 1 cut(s) 1536
FspBI CTAG 1 cut(s) 1281
GlaI GCGC 3 cut(s) 1164, 1586, 1863
GsuI CTGGAG 2 cut(s) 1140, 1455
HaeII RGCGCY 1 cut(s) 1166
HaeIII GGCC 6 cut(s) 237, 520, 564, 925, 1481, 1925
HapII CCGG 9 cut(s) 396, 516, 669, 927, 1154, 1302, 1459, 1591, 1754
HgaI GACGC 3 cut(s) 304, 363, 1118
HhaI GCGC 3 cut(s) 1165, 1587, 1864
Hin1I GRCGYC 4 cut(s) 123, 327, 363, 374
Hin1II CATG 5 cut(s) 416, 445, 712, 1889, 2198
Hin6I GCGC 3 cut(s) 1163, 1585, 1862
HinP1I GCGC 3 cut(s) 1163, 1585, 1862
HincII GTYRAC 3 cut(s) 331, 745, 1111
HindII GTYRAC 3 cut(s) 331, 745, 1111
HpaI GTTAAC 1 cut(s) 1111
HpaII CCGG 9 cut(s) 396, 516, 669, 927, 1154, 1302, 1459, 1591, 1754
HphI GGTGA 5 cut(s) 88, 473, 779, 1340, 1731
Hpy99I CGWCG 4 cut(s) 104, 329, 899, 2021
HpyAV CCTTC 8 cut(s) 175, 271, 361, 862, 1564, 1889, 2179, 2198
HpyCH4III ACNGT 6 cut(s) 100, 1022, 1150, 1269, 2098, 2276
HpyCH4IV ACGT 4 cut(s) 123, 327, 363, 1848
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 1582
HpyF3I CTNAG 3 cut(s) 528, 1549, 2226
HpySE526I ACGT 4 cut(s) 123, 327, 363, 1848
Hsp92I GRCGYC 4 cut(s) 123, 327, 363, 374
Hsp92II CATG 5 cut(s) 416, 445, 712, 1889, 2198
HspAI GCGC 3 cut(s) 1163, 1585, 1862
KflI GGGWCCC 1 cut(s) 342
KroI GCCGGC 2 cut(s) 668, 1458
KroNI GCCGGC 2 cut(s) 670, 1460
Ksp22I TGATCA 1 cut(s) 1870
KspAI GTTAAC 1 cut(s) 1111
LmnI GCTCC 7 cut(s) 116, 541, 1304, 1412, 1602, 2231, 2344
Lsp1109I GCAGC 7 cut(s) 95, 408, 411, 1152, 1568, 1569, 1952
LweI GCATC 6 cut(s) 54, 681, 703, 1166, 1762, 1849
MaeI CTAG 1 cut(s) 1281
MaeII ACGT 4 cut(s) 123, 327, 363, 1848
MaeIII GTNAC 8 cut(s) 617, 1175, 1252, 1328, 1982, 2164, 2276, 2290
MboII GAAGA 8 cut(s) 55, 121, 166, 776, 778, 1071, 1460, 1798
MfeI CAATTG 1 cut(s) 1647
MflI RGATCY 2 cut(s) 221, 634
MhlI GDGCHC 2 cut(s) 249, 1536
MlyI GAGTC 6 cut(s) 283, 539, 1131, 1206, 1541, 1767
MmeI TCCRAC 6 cut(s) 217, 1390, 1836, 2160, 2322, 2331
Mph1103I ATGCAT 1 cut(s) 1777
MroNI GCCGGC 2 cut(s) 668, 1458
MseI TTAA 5 cut(s) 1110, 1244, 1379, 2138, 2337
MslI CAYNNNNRTG 5 cut(s) 792, 1616, 1878, 1890, 2297
MspA1I CMGCKG 2 cut(s) 108, 459
MspI CCGG 9 cut(s) 396, 516, 669, 927, 1154, 1302, 1459, 1591, 1754
MspR9I CCNGG 8 cut(s) 359, 517, 825, 911, 927, 1371, 1567, 1755
MunI CAATTG 1 cut(s) 1647
Mva1269I GAATGC 2 cut(s) 304, 2213
MvaI CCWGG 5 cut(s) 359, 825, 911, 1371, 1567
MvnI CGCG 1 cut(s) 894
MwoI GCNNNNNNNGC 2 cut(s) 380, 1582
NaeI GCCGGC 2 cut(s) 670, 1460
NciI CCSGG 3 cut(s) 517, 927, 1755
NdeI CATATG 1 cut(s) 1421
NgoMIV GCCGGC 2 cut(s) 668, 1458
NlaIII CATG 5 cut(s) 416, 445, 712, 1889, 2198
NlaIV GGNNCC 5 cut(s) 343, 344, 822, 1480, 1642
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 2 cut(s) 1328, 2290
NsiI ATGCAT 1 cut(s) 1777
NspI RCATGY 1 cut(s) 1889
OliI CACNNNNGTG 1 cut(s) 2297
PceI AGGCCT 1 cut(s) 1925
PcsI WCGNNNNNNNCGW 3 cut(s) 96, 129, 240
PctI GAATGC 2 cut(s) 304, 2213
PdiI GCCGGC 2 cut(s) 670, 1460
PfeI GAWTC 5 cut(s) 597, 1428, 1468, 2130, 2317
PflMI CCANNNNNTGG 1 cut(s) 1409
PfoI TCCNGGA 1 cut(s) 1565
PinAI ACCGGT 2 cut(s) 395, 1590
Ple19I CGATCG 1 cut(s) 1137
PleI GAGTC 6 cut(s) 283, 538, 1130, 1206, 1541, 1766
PpsI GAGTC 6 cut(s) 283, 538, 1130, 1206, 1541, 1766
PpuMI RGGWCCY 1 cut(s) 342
PsiI TTATAA 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 1536
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 5 cut(s) 357, 823, 909, 1369, 1565
PspGI CCWGG 5 cut(s) 357, 823, 909, 1369, 1565
PspN4I GGNNCC 5 cut(s) 343, 344, 822, 1480, 1642
PspPI GGNCC 6 cut(s) 181, 342, 518, 820, 924, 1479
PspPPI RGGWCCY 1 cut(s) 342
PsuI RGATCY 2 cut(s) 221, 634
PvuI CGATCG 1 cut(s) 1137
RsaI GTAC 4 cut(s) 506, 938, 1310, 1996
RsaNI GTAC 4 cut(s) 505, 937, 1309, 1995
RseI CAYNNNNRTG 5 cut(s) 792, 1616, 1878, 1890, 2297
SacI GAGCTC 1 cut(s) 1536
SaqAI TTAA 5 cut(s) 1110, 1244, 1379, 2138, 2337
Sau96I GGNCC 6 cut(s) 181, 342, 518, 820, 924, 1479
SchI GAGTC 6 cut(s) 283, 539, 1131, 1206, 1541, 1767
ScrFI CCNGG 8 cut(s) 359, 517, 825, 911, 927, 1371, 1567, 1755
SduI GDGCHC 2 cut(s) 249, 1536
SfaNI GCATC 6 cut(s) 54, 681, 703, 1166, 1762, 1849
SinI GGWCC 3 cut(s) 181, 342, 820
SmiMI CAYNNNNRTG 5 cut(s) 792, 1616, 1878, 1890, 2297
SmlI CTYRAG 3 cut(s) 378, 638, 1677
SmoI CTYRAG 3 cut(s) 378, 638, 1677
SseBI AGGCCT 1 cut(s) 1925
SsiI CCGC 7 cut(s) 10, 73, 106, 387, 459, 565, 1865
SspI AATATT 3 cut(s) 974, 996, 1903
SspMI CTAG 1 cut(s) 1281
SstI GAGCTC 1 cut(s) 1536
StuI AGGCCT 1 cut(s) 1925
StyD4I CCNGG 8 cut(s) 357, 515, 823, 909, 925, 1369, 1565, 1753
StyI CCWWGG 2 cut(s) 117, 1224
TaaI ACNGT 6 cut(s) 100, 1022, 1150, 1269, 2098, 2276
TaiI ACGT 4 cut(s) 126, 330, 366, 1851
TatI WGTACW 4 cut(s) 504, 936, 1308, 1994
TauI GCSGC 3 cut(s) 390, 567, 1867
TfiI GAWTC 5 cut(s) 597, 1428, 1468, 2130, 2317
Tru1I TTAA 5 cut(s) 1110, 1244, 1379, 2138, 2337
Tru9I TTAA 5 cut(s) 1110, 1244, 1379, 2138, 2337
TscAI CASTG 6 cut(s) 47, 611, 1325, 1557, 2304, 2371
TseFI GTSAC 2 cut(s) 1328, 2290
TseI GCWGC 7 cut(s) 108, 421, 424, 1165, 1556, 1582, 1940
Tsp45I GTSAC 2 cut(s) 1328, 2290
TspDTI ATGAA 6 cut(s) 779, 864, 874, 1052, 1659, 2161
TspGWI ACGGA 4 cut(s) 710, 912, 1514, 2034
TspRI CASTG 6 cut(s) 47, 611, 1325, 1557, 2304, 2371
Van91I CCANNNNNTGG 1 cut(s) 1409
VpaK11BI GGWCC 3 cut(s) 181, 342, 820
XapI RAATTY 4 cut(s) 770, 900, 1381, 2236
XbaI TCTAGA 1 cut(s) 1280
XceI RCATGY 1 cut(s) 1889
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 548
XspI CTAG 1 cut(s) 1281
ZraI GACGTC 3 cut(s) 124, 328, 364
Zsp2I ATGCAT 1 cut(s) 1777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.