Rroxscaffold_1G00059170

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000882
Physical Location & Seq
Reverse (-)
81137509 .. 81142668
5160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059170.1

Sequence Viewer

Length: 2709 bp
ATGTCCTCAAAGAGGTCGTCGAGATCTCATAGGTTGCTTTGGAAAGACCCGGATGCACTCCCTCAAGTACCCGGCGGCGATCATCTACTCGGCGATGTTGCGGAGGTCAATCATTGCATCGGAGGGGATGAGATCGAGCTCCGGGATGCTACTTATGGACCCGTAGTTCGAGGTGTTCAACGAATAGCCTCGGCGGAGGAGGACTTGGGAGTGGAACCGCCATCATCGTCGGCGGAGAAGTCGTTGATTTCGAATTCGGCGCCGTGGTTGTTGTTGTGCTATGAAACCAAAAAAAGACTAAGAATTCGAATAACGGACTCCAATAACCAAAGATGGGAAATCCCACAAGACCTTCTCCCACGCCAAACGCCACAAACATCCCACCATTCCATCCCCGAAAGCGACCTCATTTTCACGCTCTACAACACCACCCCATTCGGCTTCACCGTCACCCGCCGTTCCTCCAAAGACGTCGTATTCGACACCACCCCAAACTCCTCCAACCCTTCCTCCATCCTCGTCTTCAAGGACCAGTACATCCAGCTTTCCTCCTCTCTTCCGGAAACCCGGTCCTCCTTGTACGGCCTCGGTGAACACACCAAACCCTCCTTCAAGCTCCAACCCTATCAGACTCTGACACTCTGGACGGCCGACATTGGCAGCGCCAACCTCGACCTCAACCTCTATGGGTCCCACCCATTTTACATGGACGTGAGGTCACCGTCAGGTGACGGAAAAGTTACCGCCGGAACCACTCATGGAGTGTTGCTGTTGAATAGTAATGGCATGGATGTAAATTACGACGGTGACAGGGTTACTTATAAGGTGATTGGCGGAGTTGTTGACTTGTATTTGTTTTCGGGTCCGACGCCGGAATTGGTGATGGAGCAGTACACAGAGCTCATTGGCCGACCTGCCCCGATGCCCTACTGGTCTTTTGATATGGTTACAAGAATGTTGCTGATATTGAGGGGGGTGGTTGCTGGTTATGCAAATGCTAGCATCCCTCTTGAAGTTATGTGGACAGACATTGACTACATGGATGCTTACAAGGATTTCACCCTTGATCCCATCAACTTTCCATTGGATAAGATGCAGAACTTTACAAACACCCTTCACCAAAATGGCCAGAAATATGTGCTCATCTTGGACCCTGGTATTAGTATAAATGAGAGCTATGGAACTTACATCCGAGGGAAGGAAGCAGATATATACATAAAACGTGATGGTATTCCTTACCAGGGAAATGTATGGCCTGGTGATGTTTACTATCCTGATTTTGTACATCCACAAAGTGAACAATTTTGGGCTAATGAGATCAAACTATTTCAAGACCAACTAGCTTTTGATGGTCTCTGGCTTGATATGAATGAGGTATCCAATTTTATAACATCTCCTCCCACTCCGAATTCCACACTTGATGATCCACCTTACAAAATCAATGATTCCGGAGTCCAGCGTCCGATCATTAGTAAGACTGTTCCGGCATCAGCTCTACACTTTGGTAATATAACAGAGTATAATGTTCATAACCTATATGGGTTCTTGGAATCTAAAGCAACTCACCAGGGGTTGATCAATGTCACTGGTAAGAGACCATTCATACTCGCTAGGTCAACCTTTGTAAGCTCTGGTAAGTACACAGCACATTGGACTGGAGACAATGCAGCAAGATGGAGTGACTTGGCATACACAATTCCAGGCATTTTGAATTTTGGACTCTTCGGAGTTCCAATGGTTGGAGCTGATATATGTGGTTTCTCTTTGAATACTACTGAAGAACTTTGCCGGCGATGGATTCAGCTAGGGGCCTTTTACCCGTTTTCACGAGATCATTCAGATAAGTTTACGATTCGCCAAGAGCTCTATCTTTGGGATTCAGTTGCTGCATCAGCAAGGAAGGTGCTAGGCCTCCGTTACCGGTTGCTCCCTTTGTTCTACACATCAATGTATCAGGCACACAAAAATGGGACCCCCATTGCAAGGCCCCTTTTCTTCTCGTTTCCTGAAGATACCAATACATACGGCACCAATTCACAGTTCTTGATTGGTAAATGTGTGATGGTGTCACCTGTGTTGCAGGAAGGAGCAAATTCAGTTAATGCATATTTTCCTGCAGGAAACTGGTTTGATCTCTTCAACTACTCCAGATCAGTGAGTGTAGACTCCGGCGAGTATGTTATGTTGGATGCACCGCCTGATCATATCAATGTACATGTCCATGAAGGAAACATATTGGCCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCTCGGAAGACTGCATTCGAACTCTTGGTGGTCATTAGTGTTAGCGGAGAGAGCACTGGAGAAGTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTCGTGAGGTTTCGTTCTGCACTAGCACAAAATGGAAGTCTAGGTTTGAGCTCAAAGGTCGAAAATGGAGAATTTGCATTAAGCCAAGGTTGGATCATTGATAAAATTACCGTCCTTGGGTGGGACAATGTTAATGGAACAGATGGGTATACATTGTGCATTACTAAAGGAGCAAAGTTGAAAGGAAACACACATGTTAAGGCCAACTTTCACAGTTATGGGCGTTTTGTCAAGGTAGAGTCCAGTGTATCCCTTCCTATAGGAAAGGAGTTTGCGTTGGAGCTGAAGCCGAATCAGATACTGGCGAAAACACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

902

Amino Acids

100.45

Weight (kDa)

5.5

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 101 - 260 4.8e-10 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 306 - 648 3.1e-109 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 657 - 746 3.1e-23 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 822, 1388
AatII GACGTC 1 cut(s) 474
Acc36I ACCTGC 1 cut(s) 922
AccB1I GGYRCC 2 cut(s) 259, 2027
AccB7I CCANNNNNTGG 1 cut(s) 1739
AccI GTMKAC 2 cut(s) 2163, 2540
AccIII TCCGGA 2 cut(s) 559, 1448
AclWI GGATC 3 cut(s) 1061, 1418, 2492
AcoI YGGCCR 3 cut(s) 648, 907, 1126
AcsI RAATTY 6 cut(s) 253, 303, 1408, 1711, 2092, 2462
AcuI CTGAAG 3 cut(s) 1797, 2028, 2696
AcyI GRCGYC 3 cut(s) 260, 471, 869
AdeI CACNNNGTG 1 cut(s) 1295
AfaI GTAC 7 cut(s) 69, 536, 581, 893, 1284, 1640, 2214
AfiI CCNNNNNNNGG 8 cut(s) 12, 334, 1198, 1241, 1739, 1983, 2508, 2512
AflIII ACRYGT 2 cut(s) 2215, 2584
AgeI ACCGGT 1 cut(s) 1920
AhdI GACNNNNNGTC 1 cut(s) 715
AjiI CACGTC 1 cut(s) 712
AjnI CCWGG 5 cut(s) 1153, 1239, 1255, 1566, 1699
AjuI GAANNNNNNNTTGG 4 cut(s) 593, 625, 2024, 2056
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
Alw21I GWGCWC 6 cut(s) 141, 903, 1143, 1866, 2327, 2444
Alw26I GTCTC 3 cut(s) 1358, 1588, 1653
AlwI GGATC 3 cut(s) 1061, 1418, 2492
AlwNI CAGNNNCTG 3 cut(s) 634, 1886, 2692
Aor13HI TCCGGA 2 cut(s) 559, 1448
ApeKI GCWGC 4 cut(s) 660, 1667, 1886, 2270
ApoI RAATTY 6 cut(s) 253, 303, 1408, 1711, 2092, 2462
ArsI GACNNNNNNTTYG 9 cut(s) 34, 150, 182, 288, 320, 461, 493, 2413, 2445
AsiGI ACCGGT 1 cut(s) 1920
AspLEI GCGC 2 cut(s) 262, 665
AspS9I GGNCC 9 cut(s) 158, 529, 570, 690, 863, 1150, 1809, 1971, 1986
AsuC2I CCSGG 4 cut(s) 50, 72, 143, 568
AsuII TTCGAA 3 cut(s) 251, 307, 2289
AsuNHI GCTAGC 1 cut(s) 998
AvaII GGWCC 7 cut(s) 158, 529, 570, 690, 863, 1150, 1971
BalI TGGCCA 1 cut(s) 1128
BanI GGYRCC 2 cut(s) 259, 2027
BanII GRGCYC 4 cut(s) 141, 903, 1866, 2444
BauI CACGAG 1 cut(s) 1827
BbsI GAAGAC 2 cut(s) 514, 2285
Bbv12I GWGCWC 6 cut(s) 141, 903, 1143, 1866, 2327, 2444
BbvI GCAGC 4 cut(s) 672, 1679, 1873, 2282
BceAI ACGGC 5 cut(s) 247, 441, 598, 663, 2041
BciT130I CCWGG 5 cut(s) 1155, 1241, 1257, 1568, 1701
BciVI GTATCC 2 cut(s) 1387, 2650
BclI TGATCA 2 cut(s) 1575, 2200
BcnI CCSGG 4 cut(s) 50, 72, 143, 568
BcoDI GTCTC 3 cut(s) 1358, 1588, 1653
BfaI CTAG 8 cut(s) 999, 1340, 1611, 1805, 1907, 2414, 2432, 2707
BfmI CTRYAG 2 cut(s) 2115, 2649
BfoI RGCGCY 2 cut(s) 263, 666
BfuAI ACCTGC 1 cut(s) 922
BfuI GTATCC 2 cut(s) 1387, 2650
BglII AGATCT 1 cut(s) 23
BisI GCNGC 5 cut(s) 76, 661, 1668, 1887, 2271
BlsI GCNGC 5 cut(s) 77, 662, 1669, 1888, 2272
Bme1390I CCNGG 9 cut(s) 50, 72, 143, 568, 1155, 1241, 1257, 1568, 1701
Bme18I GGWCC 7 cut(s) 158, 529, 570, 690, 863, 1150, 1971
BmeRI GACNNNNNGTC 1 cut(s) 715
BmgBI CACGTC 1 cut(s) 712
BmgT120I GGNCC 9 cut(s) 158, 529, 570, 690, 863, 1150, 1809, 1971, 1986
BmrFI CCNGG 9 cut(s) 50, 72, 143, 568, 1155, 1241, 1257, 1568, 1701
BmtI GCTAGC 1 cut(s) 1002
BpiI GAAGAC 2 cut(s) 514, 2285
BpmI CTGGAG 3 cut(s) 1677, 2131, 2349
Bpu14I TTCGAA 3 cut(s) 251, 307, 2289
BpuEI CTTGAG 1 cut(s) 48
BpuMI CCSGG 4 cut(s) 50, 72, 143, 568
BsaHI GRCGYC 3 cut(s) 260, 471, 869
BsaI GGTCTC 2 cut(s) 1358, 1588
BsaJI CCNNGG 9 cut(s) 189, 263, 586, 1153, 1192, 1240, 1567, 2476, 2506
BsaWI WCCGGW 3 cut(s) 559, 1448, 1920
Bsc4I CCNNNNNNNGG 8 cut(s) 12, 334, 1198, 1241, 1739, 1983, 2508, 2512
Bse118I RCCGGY 2 cut(s) 1788, 1920
Bse1I ACTGG 8 cut(s) 532, 935, 1591, 1660, 2129, 2332, 2634, 2697
Bse3DI GCAATG 2 cut(s) 112, 1977
BseAI TCCGGA 2 cut(s) 559, 1448
BseBI CCWGG 5 cut(s) 1155, 1241, 1257, 1568, 1701
BseDI CCNNGG 9 cut(s) 189, 263, 586, 1153, 1192, 1240, 1567, 2476, 2506
BseLI CCNNNNNNNGG 8 cut(s) 12, 334, 1198, 1241, 1739, 1983, 2508, 2512
BseMI GCAATG 2 cut(s) 112, 1977
BseNI ACTGG 8 cut(s) 532, 935, 1591, 1660, 2129, 2332, 2634, 2697
BseRI GAGGAG 5 cut(s) 212, 487, 541, 1386, 2385
BseX3I CGGCCG 1 cut(s) 648
BseXI GCAGC 4 cut(s) 672, 1679, 1873, 2282
BsgI GTGCAG 1 cut(s) 2394
Bsh1285I CGRYCG 1 cut(s) 651
BshNI GGYRCC 2 cut(s) 259, 2027
BshTI ACCGGT 1 cut(s) 1920
BsiEI CGRYCG 1 cut(s) 651
BsiHKAI GWGCWC 6 cut(s) 141, 903, 1143, 1866, 2327, 2444
BslFI GGGAC 3 cut(s) 676, 1984, 2528
BslI CCNNNNNNNGG 8 cut(s) 12, 334, 1198, 1241, 1739, 1983, 2508, 2512
BsmAI GTCTC 3 cut(s) 1358, 1588, 1653
BsmFI GGGAC 3 cut(s) 676, 1984, 2528
BsmI GAATGC 1 cut(s) 2285
Bso31I GGTCTC 2 cut(s) 1358, 1588
Bsp119I TTCGAA 3 cut(s) 251, 307, 2289
Bsp1286I GDGCHC 6 cut(s) 141, 903, 1143, 1866, 2327, 2444
Bsp13I TCCGGA 2 cut(s) 559, 1448
Bsp1407I TGTACA 2 cut(s) 1282, 2212
BspEI TCCGGA 2 cut(s) 559, 1448
BspMAI CTGCAG 1 cut(s) 2119
BspMI ACCTGC 1 cut(s) 922
BspOI GCTAGC 1 cut(s) 1002
BspPI GGATC 3 cut(s) 1061, 1418, 2492
BspT104I TTCGAA 3 cut(s) 251, 307, 2289
BspT107I GGYRCC 2 cut(s) 259, 2027
BspTNI GGTCTC 2 cut(s) 1358, 1588
BsrDI GCAATG 2 cut(s) 112, 1977
BsrFI RCCGGY 2 cut(s) 1788, 1920
BsrGI TGTACA 2 cut(s) 1282, 2212
BsrI ACTGG 8 cut(s) 532, 935, 1591, 1660, 2129, 2332, 2634, 2697
BssAI RCCGGY 2 cut(s) 1788, 1920
BssECI CCNNGG 9 cut(s) 189, 263, 586, 1153, 1192, 1240, 1567, 2476, 2506
BssNAI GTATAC 1 cut(s) 2541
BssNI GRCGYC 3 cut(s) 260, 471, 869
BssSI CACGAG 1 cut(s) 1827
BssT1I CCWWGG 2 cut(s) 2476, 2506
Bst1107I GTATAC 1 cut(s) 2541
Bst2BI CACGAG 1 cut(s) 1827
Bst2UI CCWGG 5 cut(s) 1155, 1241, 1257, 1568, 1701
Bst4CI ACNGT 7 cut(s) 448, 723, 806, 1480, 2040, 2503, 2606
Bst6I CTCTTC 3 cut(s) 561, 1727, 2141
BstACI GRCGYC 3 cut(s) 260, 471, 869
BstAUI TGTACA 2 cut(s) 1282, 2212
BstBI TTCGAA 3 cut(s) 251, 307, 2289
BstC8I GCNNGC 2 cut(s) 1000, 1790
BstDEI CTNAG 1 cut(s) 299
BstDSI CCRYGG 1 cut(s) 263
BstEII GGTNACC 1 cut(s) 717
BstENI CCTNNNNNAGG 1 cut(s) 10
BstH2I RGCGCY 2 cut(s) 263, 666
BstHHI GCGC 2 cut(s) 262, 665
BstMAI GTCTC 3 cut(s) 1358, 1588, 1653
BstMCI CGRYCG 1 cut(s) 651
BstMWI GCNNNNNNNGC 3 cut(s) 989, 1892, 2322
BstNI CCWGG 5 cut(s) 1155, 1241, 1257, 1568, 1701
BstNSI RCATGY 2 cut(s) 2219, 2588
BstPI GGTNACC 1 cut(s) 717
BstSCI CCNGG 9 cut(s) 48, 70, 141, 566, 1153, 1239, 1255, 1566, 1699
BstSFI CTRYAG 2 cut(s) 2115, 2649
BstV1I GCAGC 4 cut(s) 672, 1679, 1873, 2282
BstV2I GAAGAC 2 cut(s) 514, 2285
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
BstZ17I GTATAC 1 cut(s) 2541
BstZI CGGCCG 1 cut(s) 648
BsuI GTATCC 2 cut(s) 1387, 2650
BtgI CCRYGG 1 cut(s) 263
BtgZI GCGATG 2 cut(s) 108, 1807
BtrI CACGTC 1 cut(s) 712
BtsIMutI CAGTG 4 cut(s) 1584, 2160, 2325, 2641
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 2 cut(s) 1000, 1790
CaiI CAGNNNCTG 3 cut(s) 634, 1886, 2692
CfoI GCGC 2 cut(s) 262, 665
Cfr10I RCCGGY 2 cut(s) 1788, 1920
Cfr13I GGNCC 9 cut(s) 158, 529, 570, 690, 863, 1150, 1809, 1971, 1986
CseI GACGC 2 cut(s) 877, 1448
Csp6I GTAC 7 cut(s) 68, 535, 580, 892, 1283, 1639, 2213
CspAI ACCGGT 1 cut(s) 1920
CviAII CATG 7 cut(s) 706, 758, 787, 1039, 2216, 2222, 2585
CviQI GTAC 7 cut(s) 68, 535, 580, 892, 1283, 1639, 2213
DdeI CTNAG 1 cut(s) 299
DinI GGCGCC 1 cut(s) 261
DraIII CACNNNGTG 1 cut(s) 1295
DriI GACNNNNNGTC 1 cut(s) 715
EaeI YGGCCR 3 cut(s) 648, 907, 1126
EagI CGGCCG 1 cut(s) 648
Eam1104I CTCTTC 3 cut(s) 561, 1727, 2141
Eam1105I GACNNNNNGTC 1 cut(s) 715
EarI CTCTTC 3 cut(s) 561, 1727, 2141
EciI GGCGGA 3 cut(s) 209, 248, 849
Ecl136II GAGCTC 4 cut(s) 139, 901, 1864, 2442
EclXI CGGCCG 1 cut(s) 648
Eco130I CCWWGG 2 cut(s) 2476, 2506
Eco147I AGGCCT 2 cut(s) 1911, 2255
Eco24I GRGCYC 4 cut(s) 141, 903, 1866, 2444
Eco31I GGTCTC 2 cut(s) 1358, 1588
Eco47I GGWCC 7 cut(s) 158, 529, 570, 690, 863, 1150, 1971
Eco52I CGGCCG 1 cut(s) 648
Eco53kI GAGCTC 4 cut(s) 139, 901, 1864, 2442
Eco57I CTGAAG 3 cut(s) 1797, 2028, 2696
Eco91I GGTNACC 1 cut(s) 717
EcoICRI GAGCTC 4 cut(s) 139, 901, 1864, 2442
EcoNI CCTNNNNNAGG 1 cut(s) 10
EcoO109I RGGNCCY 4 cut(s) 690, 1809, 1971, 1986
EcoO65I GGTNACC 1 cut(s) 717
EcoRI GAATTC 3 cut(s) 253, 303, 1408
EcoRII CCWGG 5 cut(s) 1153, 1239, 1255, 1566, 1699
EcoT14I CCWWGG 2 cut(s) 2476, 2506
EcoT22I ATGCAT 1 cut(s) 2107
EcoT38I GRGCYC 4 cut(s) 141, 903, 1866, 2444
EgeI GGCGCC 1 cut(s) 261
EheI GGCGCC 1 cut(s) 261
ErhI CCWWGG 2 cut(s) 2476, 2506
FaeI CATG 7 cut(s) 709, 761, 790, 1042, 2219, 2225, 2588
FalI AAGNNNNNCTT 2 cut(s) 2582, 2614
FaqI GGGAC 3 cut(s) 676, 1984, 2528
FatI CATG 7 cut(s) 705, 757, 786, 1038, 2215, 2221, 2584
FauI CCCGC 1 cut(s) 461
FbaI TGATCA 2 cut(s) 1575, 2200
FblI GTMKAC 2 cut(s) 2163, 2540
Fnu4HI GCNGC 5 cut(s) 76, 661, 1668, 1887, 2271
FriOI GRGCYC 4 cut(s) 141, 903, 1866, 2444
Fsp4HI GCNGC 5 cut(s) 76, 661, 1668, 1887, 2271
FspBI CTAG 8 cut(s) 999, 1340, 1611, 1805, 1907, 2414, 2432, 2707
GlaI GCGC 2 cut(s) 261, 664
GluI GCNGC 5 cut(s) 76, 661, 1668, 1887, 2271
GsuI CTGGAG 3 cut(s) 1677, 2131, 2349
HaeII RGCGCY 2 cut(s) 263, 666
HgaI GACGC 2 cut(s) 877, 1448
HhaI GCGC 2 cut(s) 262, 665
Hin1I GRCGYC 3 cut(s) 260, 471, 869
Hin1II CATG 7 cut(s) 709, 761, 790, 1042, 2219, 2225, 2588
Hin6I GCGC 2 cut(s) 260, 663
HinP1I GCGC 2 cut(s) 260, 663
HincII GTYRAC 2 cut(s) 844, 1617
HindII GTYRAC 2 cut(s) 844, 1617
Hpy99I CGWCG 5 cut(s) 22, 232, 476, 806, 871
HpyAV CCTTC 9 cut(s) 362, 516, 619, 1124, 1192, 1894, 2078, 2219, 2654
HpyCH4III ACNGT 7 cut(s) 448, 723, 806, 1480, 2040, 2503, 2606
HpyCH4IV ACGT 3 cut(s) 471, 711, 1222
HpyF10VI GCNNNNNNNGC 3 cut(s) 989, 1892, 2322
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 3 cut(s) 471, 711, 1222
Hsp92I GRCGYC 3 cut(s) 260, 471, 869
Hsp92II CATG 7 cut(s) 709, 761, 790, 1042, 2219, 2225, 2588
HspAI GCGC 2 cut(s) 260, 663
KasI GGCGCC 1 cut(s) 259
KflI GGGWCCC 2 cut(s) 690, 1971
Kpn2I TCCGGA 2 cut(s) 559, 1448
KroI GCCGGC 1 cut(s) 1788
KroNI GCCGGC 1 cut(s) 1790
Ksp22I TGATCA 2 cut(s) 1575, 2200
LmnI GCTCC 8 cut(s) 144, 621, 886, 1742, 1932, 2087, 2561, 2671
Lsp1109I GCAGC 4 cut(s) 672, 1679, 1873, 2282
MaeI CTAG 8 cut(s) 999, 1340, 1611, 1805, 1907, 2414, 2432, 2707
MaeII ACGT 3 cut(s) 471, 711, 1222
MboII GAAGA 8 cut(s) 514, 548, 1714, 1790, 1987, 2021, 2128, 2290
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 6 cut(s) 141, 903, 1143, 1866, 2327, 2444
MlsI TGGCCA 1 cut(s) 1128
MluNI TGGCCA 1 cut(s) 1128
Mly113I GGCGCC 1 cut(s) 260
MlyI GAGTC 6 cut(s) 311, 625, 1461, 1713, 2159, 2639
MmeI TCCRAC 7 cut(s) 525, 643, 890, 1720, 2166, 2462, 2649
Mox20I TGGCCA 1 cut(s) 1128
Mph1103I ATGCAT 1 cut(s) 2107
MroI TCCGGA 2 cut(s) 559, 1448
MroNI GCCGGC 1 cut(s) 1788
MscI TGGCCA 1 cut(s) 1128
MseI TTAA 4 cut(s) 2100, 2471, 2523, 2589
MslI CAYNNNNRTG 7 cut(s) 710, 1122, 1946, 1965, 2208, 2220, 2607
Msp20I TGGCCA 1 cut(s) 1128
MspR9I CCNGG 9 cut(s) 50, 72, 143, 568, 1155, 1241, 1257, 1568, 1701
Mva1269I GAATGC 1 cut(s) 2285
MvaI CCWGG 5 cut(s) 1155, 1241, 1257, 1568, 1701
MwoI GCNNNNNNNGC 3 cut(s) 989, 1892, 2322
NaeI GCCGGC 1 cut(s) 1790
NarI GGCGCC 1 cut(s) 260
NciI CCSGG 4 cut(s) 50, 72, 143, 568
NgoMIV GCCGGC 1 cut(s) 1788
NheI GCTAGC 1 cut(s) 998
NlaIII CATG 7 cut(s) 709, 761, 790, 1042, 2219, 2225, 2588
NmeAIII GCCGAG 2 cut(s) 69, 170
NmuCI GTSAC 7 cut(s) 448, 717, 728, 806, 1582, 1679, 2067
NsiI ATGCAT 1 cut(s) 2107
NspI RCATGY 2 cut(s) 2219, 2588
NspV TTCGAA 3 cut(s) 251, 307, 2289
PceI AGGCCT 2 cut(s) 1911, 2255
PciI ACATGT 2 cut(s) 2215, 2584
PcsI WCGNNNNNNNCGW 3 cut(s) 248, 444, 477
PctI GAATGC 1 cut(s) 2285
PdiI GCCGGC 1 cut(s) 1790
PfeI GAWTC 6 cut(s) 1445, 1550, 1798, 1852, 1877, 2683
PflMI CCANNNNNTGG 1 cut(s) 1739
PfoI TCCNGGA 1 cut(s) 141
PinAI ACCGGT 1 cut(s) 1920
PkrI GCNGC 5 cut(s) 77, 662, 1669, 1888, 2272
PleI GAGTC 6 cut(s) 311, 625, 1460, 1713, 2159, 2638
PluTI GGCGCC 1 cut(s) 263
PpsI GAGTC 6 cut(s) 311, 625, 1460, 1713, 2159, 2638
PpuMI RGGWCCY 2 cut(s) 690, 1971
PscI ACATGT 2 cut(s) 2215, 2584
PsiI TTATAA 2 cut(s) 822, 1388
Psp124BI GAGCTC 4 cut(s) 141, 903, 1866, 2444
Psp5II RGGWCCY 2 cut(s) 690, 1971
Psp6I CCWGG 5 cut(s) 1153, 1239, 1255, 1566, 1699
PspEI GGTNACC 1 cut(s) 717
PspGI CCWGG 5 cut(s) 1153, 1239, 1255, 1566, 1699
PspPI GGNCC 9 cut(s) 158, 529, 570, 690, 863, 1150, 1809, 1971, 1986
PspPPI RGGWCCY 2 cut(s) 690, 1971
PstI CTGCAG 1 cut(s) 2119
PstNI CAGNNNCTG 3 cut(s) 634, 1886, 2692
PsuI RGATCY 1 cut(s) 23
RsaI GTAC 7 cut(s) 69, 536, 581, 893, 1284, 1640, 2214
RsaNI GTAC 7 cut(s) 68, 535, 580, 892, 1283, 1639, 2213
RseI CAYNNNNRTG 7 cut(s) 710, 1122, 1946, 1965, 2208, 2220, 2607
SacI GAGCTC 4 cut(s) 141, 903, 1866, 2444
SaqAI TTAA 4 cut(s) 2100, 2471, 2523, 2589
SatI GCNGC 5 cut(s) 76, 661, 1668, 1887, 2271
Sau96I GGNCC 9 cut(s) 158, 529, 570, 690, 863, 1150, 1809, 1971, 1986
SbfI CCTGCAGG 1 cut(s) 2119
SchI GAGTC 6 cut(s) 311, 625, 1461, 1713, 2159, 2639
ScrFI CCNGG 9 cut(s) 50, 72, 143, 568, 1155, 1241, 1257, 1568, 1701
SdaI CCTGCAGG 1 cut(s) 2119
SduI GDGCHC 6 cut(s) 141, 903, 1143, 1866, 2327, 2444
SfcI CTRYAG 2 cut(s) 2115, 2649
SfoI GGCGCC 1 cut(s) 261
SfuI TTCGAA 3 cut(s) 251, 307, 2289
SinI GGWCC 7 cut(s) 158, 529, 570, 690, 863, 1150, 1971
SmiMI CAYNNNNRTG 7 cut(s) 710, 1122, 1946, 1965, 2208, 2220, 2607
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
Sse8387I CCTGCAGG 1 cut(s) 2119
SseBI AGGCCT 2 cut(s) 1911, 2255
SspDI GGCGCC 1 cut(s) 259
SspMI CTAG 8 cut(s) 999, 1340, 1611, 1805, 1907, 2414, 2432, 2707
SstI GAGCTC 4 cut(s) 141, 903, 1866, 2444
StuI AGGCCT 2 cut(s) 1911, 2255
StyD4I CCNGG 9 cut(s) 48, 70, 141, 566, 1153, 1239, 1255, 1566, 1699
StyI CCWWGG 2 cut(s) 2476, 2506
TaaI ACNGT 7 cut(s) 448, 723, 806, 1480, 2040, 2503, 2606
TaiI ACGT 3 cut(s) 474, 714, 1225
TatI WGTACW 5 cut(s) 534, 891, 1282, 1638, 2212
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 6 cut(s) 1445, 1550, 1798, 1852, 1877, 2683
Tru1I TTAA 4 cut(s) 2100, 2471, 2523, 2589
Tru9I TTAA 4 cut(s) 2100, 2471, 2523, 2589
TscAI CASTG 4 cut(s) 1591, 2160, 2332, 2641
TseFI GTSAC 7 cut(s) 448, 717, 728, 806, 1582, 1679, 2067
TseI GCWGC 4 cut(s) 660, 1667, 1886, 2270
Tsp45I GTSAC 7 cut(s) 448, 717, 728, 806, 1582, 1679, 2067
TspDTI ATGAA 5 cut(s) 297, 1382, 1517, 1591, 2238
TspGWI ACGGA 3 cut(s) 329, 747, 1904
TspRI CASTG 4 cut(s) 1591, 2160, 2332, 2641
Van91I CCANNNNNTGG 1 cut(s) 1739
VpaK11BI GGWCC 7 cut(s) 158, 529, 570, 690, 863, 1150, 1971
XagI CCTNNNNNAGG 1 cut(s) 10
XapI RAATTY 6 cut(s) 253, 303, 1408, 1711, 2092, 2462
XceI RCATGY 2 cut(s) 2219, 2588
XmiI GTMKAC 2 cut(s) 2163, 2540
XspI CTAG 8 cut(s) 999, 1340, 1611, 1805, 1907, 2414, 2432, 2707
ZraI GACGTC 1 cut(s) 472
Zsp2I ATGCAT 1 cut(s) 2107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.