Rh5DG136600

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000882
Physical Location & Seq
Forward (+)
13687827 .. 13694192
6366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG136600.1

Sequence Viewer

Length: 2457 bp
ATGAACAGCTATGAAACCAAAGAAAGACTAAGAATTCGAATAACGGACTCCAATAACCAAAGATGGGAAATCCCACAAGACCTTCTCCCACGCCAGACTCCACAAACATCCCACCATCCCATTCCTGAAAGCGACCTCATTTTCACACTCCACAACACCACCCCATTCGGCTTCACCGTCACCCGCCGTTCCTCCAACGACGTCGTATTCGACACCACCCCAAACCCCTCCAACCCTTCCTCCATCCTCGTGTTCAAGGACCAGCACATCCAGCTTTCCTCCTCTCTCCCGGAAACCCGGTCCTCCTTGTACGGCCTCGGTGAACACACCAAACCCTCCTTCAAGCTCCAACCCAATCAGACCCTGACACTCTGGACTGCCGACATTGCCAGCGCCAACCTCGACCTCAACCTCTATGGGTCCCACCCGTTTCACATGGACGTGAGGTCACCGTCCGGTGACGGAAAAGTTACCGCGGGAACCACTCATGGAGTGTTGCTGTTGAATAGTAATGGCATGGATGTAAATTACGAAGGTGACAGGATTACTTATAAGGTGATTGGTGGAGTTCTGGACTTGTATTTCTTCTCGGGTCCGACGCCCGAATTGGTGATGGAGCAGTACACGGAGCTCATTGGCCGACCTGCCCCCATGCCCTACTGGTCTTTTGGATTCCACCAATGTAGATGGGGATACAAGGATGTTGCTGATGTTGAGGGGGTGGTTGCTGGTTATGCAAATGCTAGCATCCCTCTTGAAGTTATGTGGACAGACATTGACTACATGGATGCTTACAAGGATTTCACTCTTGATCCCATCAACTTTCCATTGGACAAGATGCAGAACTTTACAAACACCCTTCACCAAAATGGCCAGAAATATGTGCTCATCTTGGACCCTGGTATTAGTATAAATGAGAGCTATGGAACTTACATCAGAGGGAAGGAAGCAGATATATACATAAAACGTGATGGCATTCCTTACCAGGGAAATGTATGGCCTGGTGATGTTTACTATCCTGATTTTGTACATCCACAAAGTGAACAATTTTGGGCTAATGAGATCAAACTATTTCAAGACCAACTAGCTTTTGATGGTCTCTGGCTTGATATGAATGAGGTATCCAATTTTATAACATCTCCTCCTACTCCGAACTCCACACTTGATGATCCACCTTACAAAATCAATGATTCCGGAGTCCAGCGTCCGATCATTAATAAGACTGTTCCGGCATCAGCTCTACACTTTGGTAATATAACAGAGTATAACGTCCATAACCTATACGGGTTCTTGGAATCTAAAGCAACACACCAGGGGTTGATCAATGTTACTGGTAAGAGACCATTTATACTTGCTAGATCAACCTTTATAAGCTCTGGTAAGTACACAGCACACTGGACTGGAGACAATGCAGCAAGATGGAGTGACTTGGCATACACAACTCCAGGCATTTTGAATTTTGGACTCTTCGGAGTTCCCATGGTTGGAGCTGATATATGTGGTTTCTTTGGGGATACTAATGAAGAACTCTGCCGGCGTTGGATTCAGCTAGGGGCCTTTTACCCGTTTTCAAGAGATCATTCAGAAAAGTCTACGATCCGCCAAGAGCTCTATGTTTGGGATTCAGTCGCTGCATCAGCAAGGAAGGTGCTAGGCCTCCGTTACCGGTTGCTCCCCTTGTTCTACACATCAATGTATCAGGCACACAAAAAAGGGACCCCCATTGCAAGGCCCCTTTTCTTCTCGTTTCCTGAAGATACCAATACATACGGCATCAATTCACAGTTCTTGATTGGTAAATGTGTGATGGTGTCACCTGTGTTGCAGGAAGGAGCAAATTCAGTTCATGCATATTTTCCTGCTGGAAACTGGTTTGATCTCTTCAACTACTCCAGATCAGTGAGTGTAGACTCCGGAGAGTATGTTACGTTGGATGCACCGCCTGATCATATCAATGTACATGTCCATGAAGGAAACATATTGACCTTGCAAGGAGAGGCCTTGACAACACAAGCAGCTCGGAAGACTGCATTCGAACTCTTGGTGGTCATTAGTGTTAGCGGAGAGAGCACTGGAGAAGTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTCGTGAGGTTTCGTTCTGCACTAGCACAAAATGGAAGTCTAGTCTTGAGCTCAAAGGTCGAAAATGGAGAATTTGCATTAAGCCAAGGTTGGATCATTGATAAAATTACCGTCCTCGGGTTGGACAAAGTTAATGCAACAGATGGGTATACATTGAGCATTACTAATAAAGGAGCAAAGTTGAAAGAAAACACAAATGTTAAGGCCAACTTTCACAGTTATGAGCGTTTTGTCAAGGTAGAGTCCAGTGTATCCCTTCCTATAGGAAAGGAGTTTGAGTTGGAGCTGAAGCCGAATCAGATACTGGAGAGAACACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

818

Amino Acids

91.68

Weight (kDa)

5.35

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 11 - 170 1e-08 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 215 - 563 3.3e-121 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 572 - 661 5e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 552, 1133, 1370
AatII GACGTC 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 652
AccI GTMKAC 3 cut(s) 1592, 1908, 2285
AccII CGCG 1 cut(s) 476
AccIII TCCGGA 2 cut(s) 1193, 1913
AciI CCGC 6 cut(s) 184, 474, 476, 1600, 1940, 2061
AclWI GGATC 4 cut(s) 806, 1163, 1591, 2237
AcoI YGGCCR 2 cut(s) 637, 871
AcsI RAATTY 4 cut(s) 33, 1456, 1837, 2207
AcuI CTGAAG 2 cut(s) 1773, 2444
AcyI GRCGYC 2 cut(s) 201, 599
AdeI CACNNNGTG 1 cut(s) 1040
AfaI GTAC 5 cut(s) 311, 623, 1029, 1385, 1959
AfiI CCNNNNNNNGG 6 cut(s) 64, 986, 1484, 1728, 2253, 2257
AflIII ACRYGT 1 cut(s) 1960
AgeI ACCGGT 1 cut(s) 1665
AgsI TTSAA 9 cut(s) 256, 343, 505, 758, 1076, 1456, 1572, 1885, 2320
AhdI GACNNNNNGTC 1 cut(s) 445
AjiI CACGTC 1 cut(s) 442
AjnI CCWGG 5 cut(s) 898, 984, 1000, 1311, 1444
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
Alw21I GWGCWC 5 cut(s) 633, 888, 1611, 2072, 2189
Alw26I GTCTC 3 cut(s) 1103, 1333, 1398
AlwI GGATC 4 cut(s) 806, 1163, 1591, 2237
AlwNI CAGNNNCTG 3 cut(s) 364, 1631, 2440
Ama87I CYCGRG 2 cut(s) 589, 2252
Aor13HI TCCGGA 2 cut(s) 1193, 1913
AoxI GGCC 9 cut(s) 313, 637, 871, 998, 1554, 1654, 1730, 1998, 2340
ApeKI GCWGC 3 cut(s) 1412, 1631, 2015
ApoI RAATTY 4 cut(s) 33, 1456, 1837, 2207
ArsI GACNNNNNNTTYG 6 cut(s) 18, 50, 191, 223, 2158, 2190
AseI ATTAAT 1 cut(s) 1215
AsiGI ACCGGT 1 cut(s) 1665
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 8 cut(s) 259, 300, 420, 593, 895, 1554, 1716, 1731
AsuC2I CCSGG 2 cut(s) 290, 298
AsuII TTCGAA 2 cut(s) 37, 2034
AsuNHI GCTAGC 1 cut(s) 743
AvaI CYCGRG 2 cut(s) 589, 2252
AvaII GGWCC 6 cut(s) 259, 300, 420, 593, 895, 1716
BalI TGGCCA 1 cut(s) 873
BanII GRGCYC 3 cut(s) 633, 1611, 2189
BauI CACGAG 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 2030
Bbv12I GWGCWC 5 cut(s) 633, 888, 1611, 2072, 2189
BbvI GCAGC 3 cut(s) 1424, 1618, 2027
BceAI ACGGC 3 cut(s) 171, 328, 1786
BciT130I CCWGG 5 cut(s) 900, 986, 1002, 1313, 1446
BciVI GTATCC 4 cut(s) 686, 1132, 1507, 2398
BclI TGATCA 2 cut(s) 1320, 1945
BcnI CCSGG 2 cut(s) 290, 298
BcoDI GTCTC 3 cut(s) 1103, 1333, 1398
BfaI CTAG 8 cut(s) 744, 1085, 1356, 1550, 1652, 2159, 2177, 2455
BfmI CTRYAG 1 cut(s) 2397
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 652
BfuI GTATCC 4 cut(s) 686, 1132, 1507, 2398
BisI GCNGC 3 cut(s) 1413, 1632, 2016
BlsI GCNGC 3 cut(s) 1414, 1633, 2017
Bme1390I CCNGG 7 cut(s) 290, 298, 900, 986, 1002, 1313, 1446
Bme18I GGWCC 6 cut(s) 259, 300, 420, 593, 895, 1716
BmeRI GACNNNNNGTC 1 cut(s) 445
BmeT110I CYCGRG 2 cut(s) 589, 2252
BmgBI CACGTC 1 cut(s) 442
BmgT120I GGNCC 8 cut(s) 259, 300, 420, 593, 895, 1554, 1716, 1731
BmiI GGNNCC 9 cut(s) 421, 422, 481, 594, 897, 1555, 1717, 1718, 1733
BmrFI CCNGG 7 cut(s) 290, 298, 900, 986, 1002, 1313, 1446
BmsI GCATC 7 cut(s) 756, 778, 828, 1241, 1643, 1782, 1924
BmtI GCTAGC 1 cut(s) 747
BpiI GAAGAC 1 cut(s) 2030
BpmI CTGGAG 4 cut(s) 1422, 1428, 1876, 2094
Bpu14I TTCGAA 2 cut(s) 37, 2034
BpuEI CTTGAG 1 cut(s) 2203
BpuMI CCSGG 2 cut(s) 290, 298
BsaHI GRCGYC 2 cut(s) 201, 599
BsaI GGTCTC 2 cut(s) 1103, 1333
BsaJI CCNNGG 8 cut(s) 316, 474, 898, 985, 1312, 1479, 2221, 2251
BsaWI WCCGGW 4 cut(s) 455, 1193, 1665, 1913
Bsc4I CCNNNNNNNGG 6 cut(s) 64, 986, 1484, 1728, 2253, 2257
Bse118I RCCGGY 2 cut(s) 1533, 1665
Bse1I ACTGG 8 cut(s) 665, 1336, 1400, 1405, 1874, 2077, 2382, 2445
Bse3DI GCAATG 2 cut(s) 384, 1722
BseAI TCCGGA 2 cut(s) 1193, 1913
BseBI CCWGG 5 cut(s) 900, 986, 1002, 1313, 1446
BseDI CCNNGG 8 cut(s) 316, 474, 898, 985, 1312, 1479, 2221, 2251
BseLI CCNNNNNNNGG 6 cut(s) 64, 986, 1484, 1728, 2253, 2257
BseMI GCAATG 2 cut(s) 384, 1722
BseNI ACTGG 8 cut(s) 665, 1336, 1400, 1405, 1874, 2077, 2382, 2445
BseRI GAGGAG 3 cut(s) 271, 1131, 2130
BseXI GCAGC 3 cut(s) 1424, 1618, 2027
BsgI GTGCAG 1 cut(s) 2139
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 9 cut(s) 315, 639, 873, 1000, 1556, 1656, 1732, 2000, 2342
BshTI ACCGGT 1 cut(s) 1665
BsiHKAI GWGCWC 5 cut(s) 633, 888, 1611, 2072, 2189
BsiHKCI CYCGRG 2 cut(s) 589, 2252
BsiSI CCGG 8 cut(s) 290, 298, 456, 1194, 1229, 1534, 1666, 1914
BslFI GGGAC 2 cut(s) 406, 1729
BslI CCNNNNNNNGG 6 cut(s) 64, 986, 1484, 1728, 2253, 2257
BsmAI GTCTC 3 cut(s) 1103, 1333, 1398
BsmFI GGGAC 2 cut(s) 406, 1729
BsmI GAATGC 2 cut(s) 975, 2030
BsnI GGCC 9 cut(s) 315, 639, 873, 1000, 1556, 1656, 1732, 2000, 2342
Bso31I GGTCTC 2 cut(s) 1103, 1333
BsoBI CYCGRG 2 cut(s) 589, 2252
Bsp119I TTCGAA 2 cut(s) 37, 2034
Bsp1286I GDGCHC 5 cut(s) 633, 888, 1611, 2072, 2189
Bsp13I TCCGGA 2 cut(s) 1193, 1913
Bsp1407I TGTACA 2 cut(s) 1027, 1957
Bsp19I CCATGG 1 cut(s) 1479
BspACI CCGC 6 cut(s) 184, 474, 476, 1600, 1940, 2061
BspANI GGCC 9 cut(s) 315, 639, 873, 1000, 1556, 1656, 1732, 2000, 2342
BspEI TCCGGA 2 cut(s) 1193, 1913
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 9 cut(s) 421, 422, 481, 594, 897, 1555, 1717, 1718, 1733
BspMI ACCTGC 1 cut(s) 652
BspOI GCTAGC 1 cut(s) 747
BspPI GGATC 4 cut(s) 806, 1163, 1591, 2237
BspT104I TTCGAA 2 cut(s) 37, 2034
BspTNI GGTCTC 2 cut(s) 1103, 1333
BsrDI GCAATG 2 cut(s) 384, 1722
BsrFI RCCGGY 2 cut(s) 1533, 1665
BsrGI TGTACA 2 cut(s) 1027, 1957
BsrI ACTGG 8 cut(s) 665, 1336, 1400, 1405, 1874, 2077, 2382, 2445
BssAI RCCGGY 2 cut(s) 1533, 1665
BssECI CCNNGG 8 cut(s) 316, 474, 898, 985, 1312, 1479, 2221, 2251
BssNAI GTATAC 1 cut(s) 2286
BssNI GRCGYC 2 cut(s) 201, 599
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 2 cut(s) 1479, 2221
Bst1107I GTATAC 1 cut(s) 2286
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 5 cut(s) 900, 986, 1002, 1313, 1446
Bst4CI ACNGT 6 cut(s) 178, 453, 1225, 1785, 2248, 2354
Bst6I CTCTTC 2 cut(s) 1472, 1886
BstACI GRCGYC 2 cut(s) 201, 599
BstAUI TGTACA 2 cut(s) 1027, 1957
BstBI TTCGAA 2 cut(s) 37, 2034
BstC8I GCNNGC 3 cut(s) 391, 745, 1535
BstDEI CTNAG 1 cut(s) 29
BstDSI CCRYGG 2 cut(s) 474, 1479
BstEII GGTNACC 1 cut(s) 447
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstMAI GTCTC 3 cut(s) 1103, 1333, 1398
BstMWI GCNNNNNNNGC 5 cut(s) 271, 386, 734, 1637, 2067
BstNI CCWGG 5 cut(s) 900, 986, 1002, 1313, 1446
BstNSI RCATGY 1 cut(s) 1964
BstPI GGTNACC 1 cut(s) 447
BstSCI CCNGG 7 cut(s) 288, 296, 898, 984, 1000, 1311, 1444
BstSFI CTRYAG 1 cut(s) 2397
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 3 cut(s) 1424, 1618, 2027
BstV2I GAAGAC 1 cut(s) 2030
BstZ17I GTATAC 1 cut(s) 2286
BsuI GTATCC 4 cut(s) 686, 1132, 1507, 2398
BsuRI GGCC 9 cut(s) 315, 639, 873, 1000, 1556, 1656, 1732, 2000, 2342
BtgI CCRYGG 2 cut(s) 474, 1479
BtrI CACGTC 1 cut(s) 442
BtsIMutI CAGTG 4 cut(s) 1393, 1905, 2070, 2389
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 3 cut(s) 391, 745, 1535
CaiI CAGNNNCTG 3 cut(s) 364, 1631, 2440
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 2 cut(s) 1533, 1665
Cfr13I GGNCC 8 cut(s) 259, 300, 420, 593, 895, 1554, 1716, 1731
Cfr42I CCGCGG 1 cut(s) 477
CseI GACGC 2 cut(s) 607, 1193
Csp6I GTAC 5 cut(s) 310, 622, 1028, 1384, 1958
CspAI ACCGGT 1 cut(s) 1665
CviAII CATG 9 cut(s) 436, 488, 517, 652, 784, 1480, 1847, 1961, 1967
CviQI GTAC 5 cut(s) 310, 622, 1028, 1384, 1958
DdeI CTNAG 1 cut(s) 29
DraIII CACNNNGTG 1 cut(s) 1040
DriI GACNNNNNGTC 1 cut(s) 445
EaeI YGGCCR 2 cut(s) 637, 871
Eam1104I CTCTTC 2 cut(s) 1472, 1886
Eam1105I GACNNNNNGTC 1 cut(s) 445
EarI CTCTTC 2 cut(s) 1472, 1886
EciI GGCGGA 1 cut(s) 1589
Ecl136II GAGCTC 3 cut(s) 631, 1609, 2187
Eco130I CCWWGG 2 cut(s) 1479, 2221
Eco147I AGGCCT 2 cut(s) 1656, 2000
Eco24I GRGCYC 3 cut(s) 633, 1611, 2189
Eco31I GGTCTC 2 cut(s) 1103, 1333
Eco47I GGWCC 6 cut(s) 259, 300, 420, 593, 895, 1716
Eco53kI GAGCTC 3 cut(s) 631, 1609, 2187
Eco57I CTGAAG 2 cut(s) 1773, 2444
Eco88I CYCGRG 2 cut(s) 589, 2252
Eco91I GGTNACC 1 cut(s) 447
EcoICRI GAGCTC 3 cut(s) 631, 1609, 2187
EcoO109I RGGNCCY 4 cut(s) 420, 1554, 1716, 1731
EcoO65I GGTNACC 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 33
EcoRII CCWGG 5 cut(s) 898, 984, 1000, 1311, 1444
EcoT14I CCWWGG 2 cut(s) 1479, 2221
EcoT22I ATGCAT 1 cut(s) 1852
EcoT38I GRGCYC 3 cut(s) 633, 1611, 2189
ErhI CCWWGG 2 cut(s) 1479, 2221
FaeI CATG 9 cut(s) 439, 491, 520, 655, 787, 1483, 1850, 1964, 1970
FalI AAGNNNNNCTT 2 cut(s) 2330, 2362
FaqI GGGAC 2 cut(s) 406, 1729
FatI CATG 9 cut(s) 435, 487, 516, 651, 783, 1479, 1846, 1960, 1966
FauI CCCGC 2 cut(s) 191, 469
FbaI TGATCA 2 cut(s) 1320, 1945
FblI GTMKAC 3 cut(s) 1592, 1908, 2285
Fnu4HI GCNGC 3 cut(s) 1413, 1632, 2016
FriOI GRGCYC 3 cut(s) 633, 1611, 2189
Fsp4HI GCNGC 3 cut(s) 1413, 1632, 2016
FspBI CTAG 8 cut(s) 744, 1085, 1356, 1550, 1652, 2159, 2177, 2455
GlaI GCGC 1 cut(s) 394
GluI GCNGC 3 cut(s) 1413, 1632, 2016
GsuI CTGGAG 4 cut(s) 1422, 1428, 1876, 2094
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 9 cut(s) 315, 639, 873, 1000, 1556, 1656, 1732, 2000, 2342
HapII CCGG 8 cut(s) 290, 298, 456, 1194, 1229, 1534, 1666, 1914
HgaI GACGC 2 cut(s) 607, 1193
HhaI GCGC 1 cut(s) 395
Hin1I GRCGYC 2 cut(s) 201, 599
Hin1II CATG 9 cut(s) 439, 491, 520, 655, 787, 1483, 1850, 1964, 1970
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HpaII CCGG 8 cut(s) 290, 298, 456, 1194, 1229, 1534, 1666, 1914
Hpy166II GTNNAC 9 cut(s) 323, 624, 768, 1012, 1043, 1386, 1593, 1909, 2286
Hpy188I TCNGA 9 cut(s) 360, 597, 938, 1152, 1209, 1472, 1585, 2022, 2436
Hpy8I GTNNAC 9 cut(s) 323, 624, 768, 1012, 1043, 1386, 1593, 1909, 2286
Hpy99I CGWCG 3 cut(s) 203, 206, 601
HpyCH4III ACNGT 6 cut(s) 178, 453, 1225, 1785, 2248, 2354
HpyCH4IV ACGT 5 cut(s) 201, 441, 967, 1269, 1928
HpyF10VI GCNNNNNNNGC 5 cut(s) 271, 386, 734, 1637, 2067
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 5 cut(s) 201, 441, 967, 1269, 1928
Hsp92I GRCGYC 2 cut(s) 201, 599
Hsp92II CATG 9 cut(s) 439, 491, 520, 655, 787, 1483, 1850, 1964, 1970
HspAI GCGC 1 cut(s) 393
KflI GGGWCCC 2 cut(s) 420, 1716
Kpn2I TCCGGA 2 cut(s) 1193, 1913
KroI GCCGGC 1 cut(s) 1533
KroNI GCCGGC 1 cut(s) 1535
Ksp22I TGATCA 2 cut(s) 1320, 1945
KspI CCGCGG 1 cut(s) 477
LmnI GCTCC 8 cut(s) 351, 616, 628, 1487, 1677, 1832, 2309, 2419
Lsp1109I GCAGC 3 cut(s) 1424, 1618, 2027
LweI GCATC 7 cut(s) 756, 778, 828, 1241, 1643, 1782, 1924
MaeI CTAG 8 cut(s) 744, 1085, 1356, 1550, 1652, 2159, 2177, 2455
MaeII ACGT 5 cut(s) 201, 441, 967, 1269, 1928
MboII GAAGA 7 cut(s) 577, 1459, 1535, 1732, 1766, 1873, 2035
MhlI GDGCHC 5 cut(s) 633, 888, 1611, 2072, 2189
MlsI TGGCCA 1 cut(s) 873
MluNI TGGCCA 1 cut(s) 873
MlyI GAGTC 6 cut(s) 41, 91, 1206, 1458, 1904, 2387
Mox20I TGGCCA 1 cut(s) 873
Mph1103I ATGCAT 1 cut(s) 1852
MroI TCCGGA 2 cut(s) 1193, 1913
MroNI GCCGGC 1 cut(s) 1533
MscI TGGCCA 1 cut(s) 873
MseI TTAA 4 cut(s) 1215, 2216, 2268, 2337
MslI CAYNNNNRTG 7 cut(s) 248, 440, 867, 1691, 1953, 1965, 2355
Msp20I TGGCCA 1 cut(s) 873
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 8 cut(s) 290, 298, 456, 1194, 1229, 1534, 1666, 1914
MspR9I CCNGG 7 cut(s) 290, 298, 900, 986, 1002, 1313, 1446
Mva1269I GAATGC 2 cut(s) 975, 2030
MvaI CCWGG 5 cut(s) 900, 986, 1002, 1313, 1446
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 5 cut(s) 271, 386, 734, 1637, 2067
NaeI GCCGGC 1 cut(s) 1535
NciI CCSGG 2 cut(s) 290, 298
NcoI CCATGG 1 cut(s) 1479
NgoMIV GCCGGC 1 cut(s) 1533
NheI GCTAGC 1 cut(s) 743
NlaIII CATG 9 cut(s) 439, 491, 520, 655, 787, 1483, 1850, 1964, 1970
NlaIV GGNNCC 9 cut(s) 421, 422, 481, 594, 897, 1555, 1717, 1718, 1733
NmuCI GTSAC 6 cut(s) 178, 447, 458, 536, 1424, 1812
NsiI ATGCAT 1 cut(s) 1852
NspI RCATGY 1 cut(s) 1964
NspV TTCGAA 2 cut(s) 37, 2034
PceI AGGCCT 2 cut(s) 1656, 2000
PciI ACATGT 1 cut(s) 1960
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 207
PctI GAATGC 2 cut(s) 975, 2030
PdiI GCCGGC 1 cut(s) 1535
PfeI GAWTC 6 cut(s) 672, 1190, 1295, 1543, 1622, 2431
PfoI TCCNGGA 1 cut(s) 288
PinAI ACCGGT 1 cut(s) 1665
PkrI GCNGC 3 cut(s) 1414, 1633, 2017
PleI GAGTC 6 cut(s) 41, 91, 1205, 1458, 1904, 2386
PpsI GAGTC 6 cut(s) 41, 91, 1205, 1458, 1904, 2386
PpuMI RGGWCCY 2 cut(s) 420, 1716
PscI ACATGT 1 cut(s) 1960
PshBI ATTAAT 1 cut(s) 1215
PsiI TTATAA 3 cut(s) 552, 1133, 1370
Psp124BI GAGCTC 3 cut(s) 633, 1611, 2189
Psp5II RGGWCCY 2 cut(s) 420, 1716
Psp6I CCWGG 5 cut(s) 898, 984, 1000, 1311, 1444
PspEI GGTNACC 1 cut(s) 447
PspGI CCWGG 5 cut(s) 898, 984, 1000, 1311, 1444
PspN4I GGNNCC 9 cut(s) 421, 422, 481, 594, 897, 1555, 1717, 1718, 1733
PspPI GGNCC 8 cut(s) 259, 300, 420, 593, 895, 1554, 1716, 1731
PspPPI RGGWCCY 2 cut(s) 420, 1716
PstNI CAGNNNCTG 3 cut(s) 364, 1631, 2440
RsaI GTAC 5 cut(s) 311, 623, 1029, 1385, 1959
RsaNI GTAC 5 cut(s) 310, 622, 1028, 1384, 1958
RseI CAYNNNNRTG 7 cut(s) 248, 440, 867, 1691, 1953, 1965, 2355
SacI GAGCTC 3 cut(s) 633, 1611, 2189
SacII CCGCGG 1 cut(s) 477
SaqAI TTAA 4 cut(s) 1215, 2216, 2268, 2337
SatI GCNGC 3 cut(s) 1413, 1632, 2016
Sau96I GGNCC 8 cut(s) 259, 300, 420, 593, 895, 1554, 1716, 1731
SchI GAGTC 6 cut(s) 41, 91, 1206, 1458, 1904, 2387
ScrFI CCNGG 7 cut(s) 290, 298, 900, 986, 1002, 1313, 1446
SduI GDGCHC 5 cut(s) 633, 888, 1611, 2072, 2189
SfaNI GCATC 7 cut(s) 756, 778, 828, 1241, 1643, 1782, 1924
SfcI CTRYAG 1 cut(s) 2397
Sfr303I CCGCGG 1 cut(s) 477
SfuI TTCGAA 2 cut(s) 37, 2034
SgrBI CCGCGG 1 cut(s) 477
SinI GGWCC 6 cut(s) 259, 300, 420, 593, 895, 1716
SmiMI CAYNNNNRTG 7 cut(s) 248, 440, 867, 1691, 1953, 1965, 2355
SmlI CTYRAG 1 cut(s) 2182
SmoI CTYRAG 1 cut(s) 2182
SseBI AGGCCT 2 cut(s) 1656, 2000
SsiI CCGC 6 cut(s) 184, 474, 476, 1600, 1940, 2061
SspMI CTAG 8 cut(s) 744, 1085, 1356, 1550, 1652, 2159, 2177, 2455
SstI GAGCTC 3 cut(s) 633, 1611, 2189
StuI AGGCCT 2 cut(s) 1656, 2000
StyD4I CCNGG 7 cut(s) 288, 296, 898, 984, 1000, 1311, 1444
StyI CCWWGG 2 cut(s) 1479, 2221
TaaI ACNGT 6 cut(s) 178, 453, 1225, 1785, 2248, 2354
TaiI ACGT 5 cut(s) 204, 444, 970, 1272, 1931
TaqI TCGA 6 cut(s) 37, 210, 402, 2034, 2106, 2196
TatI WGTACW 4 cut(s) 621, 1027, 1383, 1957
TfiI GAWTC 6 cut(s) 672, 1190, 1295, 1543, 1622, 2431
Tru1I TTAA 4 cut(s) 1215, 2216, 2268, 2337
Tru9I TTAA 4 cut(s) 1215, 2216, 2268, 2337
TscAI CASTG 4 cut(s) 1400, 1905, 2077, 2389
TseFI GTSAC 6 cut(s) 178, 447, 458, 536, 1424, 1812
TseI GCWGC 3 cut(s) 1412, 1631, 2015
Tsp45I GTSAC 6 cut(s) 178, 447, 458, 536, 1424, 1812
TspDTI ATGAA 6 cut(s) 17, 27, 1127, 1536, 1835, 1983
TspGWI ACGGA 4 cut(s) 59, 477, 641, 1649
TspRI CASTG 4 cut(s) 1400, 1905, 2077, 2389
VpaK11BI GGWCC 6 cut(s) 259, 300, 420, 593, 895, 1716
VspI ATTAAT 1 cut(s) 1215
XapI RAATTY 4 cut(s) 33, 1456, 1837, 2207
XceI RCATGY 1 cut(s) 1964
XmiI GTMKAC 3 cut(s) 1592, 1908, 2285
XspI CTAG 8 cut(s) 744, 1085, 1356, 1550, 1652, 2159, 2177, 2455
ZraI GACGTC 1 cut(s) 202
Zsp2I ATGCAT 1 cut(s) 1852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.