MD05G1262300.v1.1

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000882
Physical Location & Seq
Reverse (-)
39729391 .. 39733637
4247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1262300.v1.1.491

Sequence Viewer

Length: 2700 bp
ATGGATCGTCGTCTGCATCTGCCATTCTTCTTCTTCTTCTTGTCTGTTTTCTATCTTGCATCATCTGCAAAAGCAATTGCAGAAGCAGAAGCAGCAGTTGGGTATGGATACAGAATTCTATCTGTCAACTACTACGATTCCTCCAGGAACTCATTGACCGCTAATCTTGGCCTCATCAAGCCCTCCTCCCTCTACGGACCTGACATCCCAAATCTCAATCTTCACGCCAGCTGTGAAACCAAAGATCGTCTAAGAATTCGAATTACAGACTCAAAGCAACAACGATGGGAAATCCCACAAAACATGATTCCCCATCAAACCAAAACGCCCACCCCCACCCCCCACTGCATCTCCTCCAGCGACTTTGTCTTCACTCTTCGCAACACCACCCCATTCGGCTTCACTATCACCCGCCGCTCCTCCAACGACGTCATCTTCGACACCTCCCCCAAATCATCAGACCCCAACACCTTCTTGGTCTTCAAGGACCAATACATCCAACTCTCCTCTTCTCTCCCCCAAAACAGATCCTCCATTTTCGGCCTCGGCGAGCACACCAAGTCCTCCTTCAAGATCATTCCCAACCAAACATTGACTCTCTGGAATGCTGACATTGGTAGCGTCAATCCCGACCTCAACCTTTACGGGTCCCACCCTTTCTACCTGGACGTCAGGTCCGCGTCTCAAGATGGCGGCAAGACCGGTGCAGGAACATCTCATGGGGTGTTGCTGCTGAATAGTAATGGCATGGACATAACATACAGCGGTGCTAGGATTACTTATAAGGTAATTGGTGGAATTGTGGACTTGTACTTCTTTTCTGGACCGACACCTGAGTTGGTGGTAGAGCAATATACAGAACTCATTGGTCGCCCTACCCCTATGCCCTACTGGTCTTTTGGATTCCACCAGTGTCGATATGGTTACAAGAATGTTTCCGATGTTGAGGGCGTGGTTGCTGGTTATGCAAAAGCCAGTATCCCTCTTGAAGTTATGTGGACGGACATTGATTACATGGATGCTTACAAGGACTTCACTCTCGATCCCGTCAACTTTCCATTGGATAGGATGAAGAAATTTGTTAACACTCTTCACCAAAATGGTCAGAAATATGTCCTCATCTTGGATCCTGGTATCAGTAACAATGATACCTATGAAACCTACATCAGAGGGAAGAAAGCAGATATATTCATCAAGCGCAATGGAATTCCTTACCTGGGAAGTGTATGGCCTGGTGATGTCTACTTCCCTGACTTTGCACATCCACGTACTGAAAAATATTGGGCTAATGAGATACAAATATTTCAAGATGCTCTGCCTTTTGACGGTCTTTGGCTCGACATGAATGAGATATCCAATTTCATAACCTCTCCTCCCACTCCATCTTCTACACTTGATGACCCTCCTTTCAAGATTAACAATTCTGGAGTCCTGCGTCCCATCAATAACAAGACTGTTCCGGCATCAACGCTGCACTTTGGTAACATTACAGAATATAATGCACATAACCTGTATGGACTCTTGGAAGCCAAGGCAACAAACGAGGCCTTAATCAATGTAACTGGTAAAAGACCGTTTATACTTTCTAGATCAACTTTTGTAAGCTCCGGTAAGTACACAGCACACTGGACTGGAGATAATTCTGCACAATGGAGTGATCTAGCATACACAATTCCAGGCATTCTAAATTTTGGACTTTTCGGAGTTCCGATGGTTGGAGCTGATATATGTGGATTCTCTGGAAATACTACTGAAGAACTCTGCCGGCGATGGATTCAGCTAGGGGCCTTTTACCCCTTTTCAAGAGATCACTCAGACAAGTTTACAATCCGTCAAGAGCTCTATCTATGGGATTCAGTGGCAGAAACATCCAGGAAGGTTCTTGGGCTGCGCTATCGGTTGCTCCCCTTATTTTACACATCAATGTATGAGGCACACAAAAAGGGAACCCCAATTGCAAGACCCCTTTTCTTCTCATTTCCTCAAGATACCACAACTTACGAGGTCAATACGCAGTTTCTGATTGGTAGAGGTGTAATGGTATCACCTGTGTTGAAGCCAGGAGTCAATTCAGTTGATGCATATTTTCCCGCTGGAAACTGGTTTGATCTCTTCAACTACTCGAAATCAGTAAGTGTAAGTTCAGGACAGTACATTAGGCTGGATGCGCCGCCTGATCACATCAACGTGCATGTCCACGAAGGAAATATTTTGGCGTTGCAAGGGGAGGCCTTGACAACACAAGCGGCCCGAAAGACTGCATTCGAGCTCTTGGTAGTTTTTAGTAGCAGCGGACAAATTACAGGAGAAGTTTTCTTGGACGACGGAGAGGAAATTGAAATGGGAGGAGAGGGAGGTAAGTGGAGTTTAGTGAGATTTAATAGTGGAACAGAAAAGGGAAGTGTATTTGTAAGCTCAAATGTTGTAAATGGAGGATTCGCTTTAAGCGAAAAATGGATCATTGATAAAGTTACCTTCATTGGGTTGGAAAAGGTTGGTCATGGATTTGAAGGGTATGCGCTCAACATTGCTAAGGGAGCGAATTTGAAGGGAAACTCAGACATGAGAGCAAGCTTTGACAGGAATAAGCAGTTTGTCACAGTAGTGATTTCCGACCTTTCGATTCTTATAGGAGCAGAGTTTAAGTTGGAGTTGAAGCCGAAACATACTGCTGAACGCACTTTGCAGAGAACAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

900

Amino Acids

100.43

Weight (kDa)

6.49

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 83 - 226 1.7e-10 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 293 - 640 1.1e-122 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 649 - 738 6.2e-24 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 783
AatII GACGTC 2 cut(s) 432, 672
AccBSI CCGCTC 1 cut(s) 417
AccI GTMKAC 1 cut(s) 1242
AccII CGCG 1 cut(s) 680
AclWI GGATC 6 cut(s) 12, 522, 1037, 1121, 1134, 2465
AcsI RAATTY 6 cut(s) 114, 255, 1076, 1206, 1687, 2542
AcuI CTGAAG 1 cut(s) 1773
AcyI GRCGYC 2 cut(s) 429, 669
AfaI GTAC 4 cut(s) 812, 1270, 1616, 2153
AfiI CCNNNNNNNGG 5 cut(s) 1123, 1217, 1325, 1715, 2481
AgeI ACCGGT 1 cut(s) 701
AhdI GACNNNNNGTC 1 cut(s) 673
AjnI CCWGG 8 cut(s) 143, 663, 1129, 1215, 1231, 1675, 1871, 2059
AjuI GAANNNNNNNTTGG 4 cut(s) 81, 113, 551, 583
AleI CACNNNNGTG 1 cut(s) 2603
AluBI AGCT 8 cut(s) 231, 1605, 1721, 1780, 1840, 2269, 2415, 2574
AluI AGCT 8 cut(s) 231, 1605, 1721, 1780, 1840, 2269, 2415, 2574
Alw21I GWGCWC 3 cut(s) 555, 1842, 2271
Alw26I GTCTC 1 cut(s) 687
AlwI GGATC 6 cut(s) 12, 522, 1037, 1121, 1134, 2465
AlwNI CAGNNNCTG 1 cut(s) 2020
AoxI GGCC 7 cut(s) 169, 541, 1229, 1545, 1785, 2229, 2247
ApeKI GCWGC 5 cut(s) 92, 730, 1471, 1888, 2289
ApoI RAATTY 6 cut(s) 114, 255, 1076, 1206, 1687, 2542
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
AsiGI ACCGGT 1 cut(s) 701
AspLEI GCGC 4 cut(s) 1200, 1893, 2170, 2521
AspS9I GGNCC 7 cut(s) 197, 487, 648, 675, 824, 1785, 2248
AsuHPI GGTGA 4 cut(s) 400, 1085, 1247, 2037
AsuII TTCGAA 1 cut(s) 259
AvaII GGWCC 5 cut(s) 197, 487, 648, 675, 824
BamHI GGATCC 1 cut(s) 1126
BanII GRGCYC 2 cut(s) 1842, 2271
BbsI GAAGAC 2 cut(s) 361, 472
Bbv12I GWGCWC 3 cut(s) 555, 1842, 2271
BbvI GCAGC 5 cut(s) 104, 717, 1458, 1875, 2301
BccI CCATC 7 cut(s) 279, 321, 683, 1390, 1448, 1705, 1764
BciT130I CCWGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
BciVI GTATCC 2 cut(s) 101, 989
BclI TGATCA 1 cut(s) 2176
BcoDI GTCTC 1 cut(s) 687
BfaI CTAG 4 cut(s) 771, 1587, 1661, 1781
BfuI GTATCC 2 cut(s) 101, 989
BisI GCNGC 9 cut(s) 93, 415, 694, 731, 1472, 1889, 2171, 2247, 2290
BlsI GCNGC 9 cut(s) 94, 416, 695, 732, 1473, 1890, 2172, 2248, 2291
Bme1390I CCNGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
Bme18I GGWCC 5 cut(s) 197, 487, 648, 675, 824
BmeRI GACNNNNNGTC 1 cut(s) 673
BmgT120I GGNCC 7 cut(s) 197, 487, 648, 675, 824, 1785, 2248
BmiI GGNNCC 5 cut(s) 649, 650, 1128, 1786, 1948
BmrFI CCNGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
BmsI GCATC 8 cut(s) 25, 68, 357, 1009, 1300, 1472, 2068, 2155
BpiI GAAGAC 2 cut(s) 361, 472
BpmI CTGGAG 4 cut(s) 127, 340, 1446, 1653
Bpu10I CCTNAGC 1 cut(s) 2532
Bpu14I TTCGAA 1 cut(s) 259
BpuEI CTTGAG 2 cut(s) 669, 1968
BsaAI YACGTR 1 cut(s) 1268
BsaHI GRCGYC 2 cut(s) 429, 669
BsaJI CCNNGG 3 cut(s) 544, 1216, 1530
BsaWI WCCGGW 2 cut(s) 701, 1607
BsaXI ACNNNNNCTCC 8 cut(s) 125, 155, 335, 365, 515, 545, 1357, 1387
Bsc4I CCNNNNNNNGG 5 cut(s) 1123, 1217, 1325, 1715, 2481
Bse118I RCCGGY 2 cut(s) 701, 1764
Bse1I ACTGG 7 cut(s) 896, 910, 975, 1567, 1631, 1636, 2105
Bse3DI GCAATG 2 cut(s) 1207, 2526
BseBI CCWGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
BseDI CCNNGG 3 cut(s) 544, 1216, 1530
BseGI GGATG 7 cut(s) 204, 495, 1024, 1074, 1261, 1868, 2170
BseLI CCNNNNNNNGG 5 cut(s) 1123, 1217, 1325, 1715, 2481
BseMI GCAATG 2 cut(s) 1207, 2526
BseMII CTCAG 3 cut(s) 825, 1827, 2571
BseNI ACTGG 7 cut(s) 896, 910, 975, 1567, 1631, 1636, 2105
BseRI GAGGAG 6 cut(s) 175, 343, 409, 496, 1362, 2361
BseXI GCAGC 5 cut(s) 104, 717, 1458, 1875, 2301
BsgI GTGCAG 3 cut(s) 726, 1457, 1629
Bsh1236I CGCG 1 cut(s) 680
BshFI GGCC 7 cut(s) 171, 543, 1231, 1547, 1787, 2231, 2249
BshTI ACCGGT 1 cut(s) 701
BsiHKAI GWGCWC 3 cut(s) 555, 1842, 2271
BsiSI CCGG 4 cut(s) 702, 1460, 1608, 1765
BslFI GGGAC 2 cut(s) 634, 1422
BslI CCNNNNNNNGG 5 cut(s) 1123, 1217, 1325, 1715, 2481
BsmAI GTCTC 1 cut(s) 687
BsmBI CGTCTC 1 cut(s) 687
BsmFI GGGAC 2 cut(s) 634, 1422
BsmI GAATGC 3 cut(s) 610, 1680, 2261
BsnI GGCC 7 cut(s) 171, 543, 1231, 1547, 1787, 2231, 2249
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 555, 1842, 2271
BspANI GGCC 7 cut(s) 171, 543, 1231, 1547, 1787, 2231, 2249
BspCNI CTCAG 3 cut(s) 826, 1826, 2570
BspFNI CGCG 1 cut(s) 680
BspLI GGNNCC 5 cut(s) 649, 650, 1128, 1786, 1948
BspPI GGATC 6 cut(s) 12, 522, 1037, 1121, 1134, 2465
BspT104I TTCGAA 1 cut(s) 259
BsrBI CCGCTC 1 cut(s) 417
BsrDI GCAATG 2 cut(s) 1207, 2526
BsrFI RCCGGY 2 cut(s) 701, 1764
BsrI ACTGG 7 cut(s) 896, 910, 975, 1567, 1631, 1636, 2105
BssAI RCCGGY 2 cut(s) 701, 1764
BssECI CCNNGG 3 cut(s) 544, 1216, 1530
BssNI GRCGYC 2 cut(s) 429, 669
BssT1I CCWWGG 1 cut(s) 1530
Bst2UI CCWGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
Bst4CI ACNGT 5 cut(s) 1328, 1456, 1575, 2151, 2602
Bst6I CTCTTC 4 cut(s) 381, 514, 1095, 2117
BstACI GRCGYC 2 cut(s) 429, 669
BstAPI GCANNNNNTGC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 1268
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 4 cut(s) 229, 551, 1766, 2572
BstDEI CTNAG 5 cut(s) 251, 834, 1813, 2532, 2557
BstF5I GGATG 7 cut(s) 204, 495, 1024, 1074, 1261, 1868, 2170
BstFNI CGCG 1 cut(s) 680
BstHHI GCGC 4 cut(s) 1200, 1893, 2170, 2521
BstMAI GTCTC 1 cut(s) 687
BstMWI GCNNNNNNNGC 5 cut(s) 65, 92, 965, 2167, 2537
BstNI CCWGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
BstNSI RCATGY 1 cut(s) 2195
BstSCI CCNGG 8 cut(s) 143, 663, 1129, 1215, 1231, 1675, 1871, 2059
BstUI CGCG 1 cut(s) 680
BstV1I GCAGC 5 cut(s) 104, 717, 1458, 1875, 2301
BstV2I GAAGAC 2 cut(s) 361, 472
BstX2I RGATCY 2 cut(s) 527, 1126
BstYI RGATCY 2 cut(s) 527, 1126
BsuI GTATCC 2 cut(s) 101, 989
BsuRI GGCC 7 cut(s) 171, 543, 1231, 1547, 1787, 2231, 2249
BtgZI GCGATG 1 cut(s) 1783
BtsCI GGATG 7 cut(s) 204, 495, 1024, 1074, 1261, 1868, 2170
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 4 cut(s) 343, 917, 1624, 1863
Cac8I GCNNGC 4 cut(s) 229, 551, 1766, 2572
CaiI CAGNNNCTG 1 cut(s) 2020
CfoI GCGC 4 cut(s) 1200, 1893, 2170, 2521
Cfr10I RCCGGY 2 cut(s) 701, 1764
Cfr13I GGNCC 7 cut(s) 197, 487, 648, 675, 824, 1785, 2248
CseI GACGC 3 cut(s) 610, 669, 1424
Csp6I GTAC 4 cut(s) 811, 1269, 1615, 2152
CspAI ACCGGT 1 cut(s) 701
CviAII CATG 8 cut(s) 304, 719, 748, 1015, 1342, 2192, 2501, 2563
CviQI GTAC 4 cut(s) 811, 1269, 1615, 2152
DdeI CTNAG 5 cut(s) 251, 834, 1813, 2532, 2557
DriI GACNNNNNGTC 1 cut(s) 673
Eam1104I CTCTTC 4 cut(s) 381, 514, 1095, 2117
Eam1105I GACNNNNNGTC 1 cut(s) 673
EarI CTCTTC 4 cut(s) 381, 514, 1095, 2117
Ecl136II GAGCTC 2 cut(s) 1840, 2269
Eco130I CCWWGG 1 cut(s) 1530
Eco147I AGGCCT 2 cut(s) 1547, 2231
Eco24I GRGCYC 2 cut(s) 1842, 2271
Eco32I GATATC 1 cut(s) 1353
Eco47I GGWCC 5 cut(s) 197, 487, 648, 675, 824
Eco53kI GAGCTC 2 cut(s) 1840, 2269
Eco57I CTGAAG 1 cut(s) 1773
EcoICRI GAGCTC 2 cut(s) 1840, 2269
EcoO109I RGGNCCY 2 cut(s) 648, 1785
EcoRI GAATTC 3 cut(s) 114, 255, 1206
EcoRII CCWGG 8 cut(s) 143, 663, 1129, 1215, 1231, 1675, 1871, 2059
EcoRV GATATC 1 cut(s) 1353
EcoT14I CCWWGG 1 cut(s) 1530
EcoT22I ATGCAT 1 cut(s) 2083
EcoT38I GRGCYC 2 cut(s) 1842, 2271
ErhI CCWWGG 1 cut(s) 1530
Esp3I CGTCTC 1 cut(s) 687
FaeI CATG 8 cut(s) 307, 722, 751, 1018, 1345, 2195, 2504, 2566
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 2 cut(s) 634, 1422
FatI CATG 8 cut(s) 303, 718, 747, 1014, 1341, 2191, 2500, 2562
FauI CCCGC 2 cut(s) 419, 2098
FbaI TGATCA 1 cut(s) 2176
FblI GTMKAC 1 cut(s) 1242
Fnu4HI GCNGC 9 cut(s) 93, 415, 694, 731, 1472, 1889, 2171, 2247, 2290
FokI GGATG 7 cut(s) 191, 482, 1031, 1081, 1248, 1855, 2177
FriOI GRGCYC 2 cut(s) 1842, 2271
Fsp4HI GCNGC 9 cut(s) 93, 415, 694, 731, 1472, 1889, 2171, 2247, 2290
FspBI CTAG 4 cut(s) 771, 1587, 1661, 1781
GlaI GCGC 4 cut(s) 1199, 1892, 2169, 2520
GluI GCNGC 9 cut(s) 93, 415, 694, 731, 1472, 1889, 2171, 2247, 2290
GsuI CTGGAG 4 cut(s) 127, 340, 1446, 1653
HaeIII GGCC 7 cut(s) 171, 543, 1231, 1547, 1787, 2231, 2249
HapII CCGG 4 cut(s) 702, 1460, 1608, 1765
HgaI GACGC 3 cut(s) 610, 669, 1424
HhaI GCGC 4 cut(s) 1200, 1893, 2170, 2521
Hin1I GRCGYC 2 cut(s) 429, 669
Hin1II CATG 8 cut(s) 307, 722, 751, 1018, 1345, 2195, 2504, 2566
Hin6I GCGC 4 cut(s) 1198, 1891, 2168, 2519
HinP1I GCGC 4 cut(s) 1198, 1891, 2168, 2519
HincII GTYRAC 3 cut(s) 127, 1051, 1084
HindII GTYRAC 3 cut(s) 127, 1051, 1084
HindIII AAGCTT 1 cut(s) 2572
HpaI GTTAAC 1 cut(s) 1084
HpaII CCGG 4 cut(s) 702, 1460, 1608, 1765
HphI GGTGA 4 cut(s) 400, 1085, 1247, 2037
Hpy166II GTNNAC 9 cut(s) 127, 805, 999, 1051, 1084, 1243, 1617, 1824, 2197
Hpy8I GTNNAC 9 cut(s) 127, 805, 999, 1051, 1084, 1243, 1617, 1824, 2197
Hpy99I CGWCG 3 cut(s) 12, 431, 2327
HpyAV CCTTC 7 cut(s) 481, 577, 1870, 2195, 2485, 2504, 2542
HpyCH4III ACNGT 5 cut(s) 1328, 1456, 1575, 2151, 2602
HpyCH4IV ACGT 4 cut(s) 429, 669, 1267, 2187
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 92, 965, 2167, 2537
HpyF3I CTNAG 5 cut(s) 251, 834, 1813, 2532, 2557
HpySE526I ACGT 4 cut(s) 429, 669, 1267, 2187
Hsp92I GRCGYC 2 cut(s) 429, 669
Hsp92II CATG 8 cut(s) 307, 722, 751, 1018, 1345, 2195, 2504, 2566
HspAI GCGC 4 cut(s) 1198, 1891, 2168, 2519
KflI GGGWCCC 1 cut(s) 648
KroI GCCGGC 1 cut(s) 1764
KroNI GCCGGC 1 cut(s) 1766
Ksp22I TGATCA 1 cut(s) 2176
KspAI GTTAAC 1 cut(s) 1084
LmnI GCTCC 6 cut(s) 422, 1610, 1718, 1908, 2537, 2633
Lsp1109I GCAGC 5 cut(s) 104, 717, 1458, 1875, 2301
LweI GCATC 8 cut(s) 25, 68, 357, 1009, 1300, 1472, 2068, 2155
MaeI CTAG 4 cut(s) 771, 1587, 1661, 1781
MaeII ACGT 4 cut(s) 429, 669, 1267, 2187
MaeIII GTNAC 6 cut(s) 923, 1139, 1481, 1558, 2470, 2596
MbiI CCGCTC 1 cut(s) 417
MfeI CAATTG 2 cut(s) 75, 1953
MflI RGATCY 2 cut(s) 527, 1126
MhlI GDGCHC 3 cut(s) 555, 1842, 2271
MlyI GAGTC 5 cut(s) 263, 589, 1437, 1512, 2073
MmeI TCCRAC 6 cut(s) 447, 523, 1696, 2466, 2628, 2637
Mph1103I ATGCAT 1 cut(s) 2083
MroNI GCCGGC 1 cut(s) 1764
MseI TTAA 6 cut(s) 1083, 1416, 1550, 2379, 2444, 2643
MslI CAYNNNNRTG 4 cut(s) 1098, 1922, 2186, 2603
MspA1I CMGCKG 4 cut(s) 231, 765, 2093, 2292
MspI CCGG 4 cut(s) 702, 1460, 1608, 1765
MspR9I CCNGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
MunI CAATTG 2 cut(s) 75, 1953
Mva1269I GAATGC 3 cut(s) 610, 1680, 2261
MvaI CCWGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
MvnI CGCG 1 cut(s) 680
MwoI GCNNNNNNNGC 5 cut(s) 65, 92, 965, 2167, 2537
NaeI GCCGGC 1 cut(s) 1766
NgoMIV GCCGGC 1 cut(s) 1764
NlaIII CATG 8 cut(s) 307, 722, 751, 1018, 1345, 2195, 2504, 2566
NlaIV GGNNCC 5 cut(s) 649, 650, 1128, 1786, 1948
NmeAIII GCCGAG 1 cut(s) 525
NmuCI GTSAC 1 cut(s) 2596
NsiI ATGCAT 1 cut(s) 2083
NspI RCATGY 1 cut(s) 2195
NspV TTCGAA 1 cut(s) 259
OliI CACNNNNGTG 1 cut(s) 2603
PceI AGGCCT 2 cut(s) 1547, 2231
PcsI WCGNNNNNNNCGW 3 cut(s) 435, 546, 2445
PctI GAATGC 3 cut(s) 610, 1680, 2261
PdiI GCCGGC 1 cut(s) 1766
PfeI GAWTC 8 cut(s) 137, 307, 903, 1734, 1774, 1853, 2436, 2623
PflFI GACNNNGTC 1 cut(s) 365
PfoI TCCNGGA 2 cut(s) 143, 1871
PinAI ACCGGT 1 cut(s) 701
PkrI GCNGC 9 cut(s) 94, 416, 695, 732, 1473, 1890, 2172, 2248, 2291
PleI GAGTC 5 cut(s) 263, 589, 1436, 1512, 2072
PpsI GAGTC 5 cut(s) 263, 589, 1436, 1512, 2072
Ppu21I YACGTR 1 cut(s) 1268
PpuMI RGGWCCY 1 cut(s) 648
PsiI TTATAA 1 cut(s) 783
Psp124BI GAGCTC 2 cut(s) 1842, 2271
Psp5II RGGWCCY 1 cut(s) 648
Psp6I CCWGG 8 cut(s) 143, 663, 1129, 1215, 1231, 1675, 1871, 2059
PspGI CCWGG 8 cut(s) 143, 663, 1129, 1215, 1231, 1675, 1871, 2059
PspN4I GGNNCC 5 cut(s) 649, 650, 1128, 1786, 1948
PspPI GGNCC 7 cut(s) 197, 487, 648, 675, 824, 1785, 2248
PspPPI RGGWCCY 1 cut(s) 648
PstNI CAGNNNCTG 1 cut(s) 2020
PsuI RGATCY 2 cut(s) 527, 1126
PsyI GACNNNGTC 1 cut(s) 365
PvuII CAGCTG 1 cut(s) 231
RsaI GTAC 4 cut(s) 812, 1270, 1616, 2153
RsaNI GTAC 4 cut(s) 811, 1269, 1615, 2152
RseI CAYNNNNRTG 4 cut(s) 1098, 1922, 2186, 2603
SacI GAGCTC 2 cut(s) 1842, 2271
SaqAI TTAA 6 cut(s) 1083, 1416, 1550, 2379, 2444, 2643
SatI GCNGC 9 cut(s) 93, 415, 694, 731, 1472, 1889, 2171, 2247, 2290
Sau96I GGNCC 7 cut(s) 197, 487, 648, 675, 824, 1785, 2248
SchI GAGTC 5 cut(s) 263, 589, 1437, 1512, 2073
ScrFI CCNGG 8 cut(s) 145, 665, 1131, 1217, 1233, 1677, 1873, 2061
SduI GDGCHC 3 cut(s) 555, 1842, 2271
SfaNI GCATC 8 cut(s) 25, 68, 357, 1009, 1300, 1472, 2068, 2155
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 5 cut(s) 197, 487, 648, 675, 824
SmiMI CAYNNNNRTG 4 cut(s) 1098, 1922, 2186, 2603
SmlI CTYRAG 2 cut(s) 684, 1983
SmoI CTYRAG 2 cut(s) 684, 1983
SseBI AGGCCT 2 cut(s) 1547, 2231
SspI AATATT 3 cut(s) 1280, 1302, 2209
SspMI CTAG 4 cut(s) 771, 1587, 1661, 1781
SstI GAGCTC 2 cut(s) 1842, 2271
StuI AGGCCT 2 cut(s) 1547, 2231
StyD4I CCNGG 8 cut(s) 143, 663, 1129, 1215, 1231, 1675, 1871, 2059
StyI CCWWGG 1 cut(s) 1530
TaaI ACNGT 5 cut(s) 1328, 1456, 1575, 2151, 2602
TaiI ACGT 4 cut(s) 432, 672, 1270, 2190
TaqI TCGA 8 cut(s) 259, 438, 916, 1041, 1338, 2123, 2265, 2621
TaqII GACCGA 1 cut(s) 841
TatI WGTACW 3 cut(s) 810, 1614, 2151
TauI GCSGC 4 cut(s) 417, 696, 2173, 2249
TfiI GAWTC 8 cut(s) 137, 307, 903, 1734, 1774, 1853, 2436, 2623
Tru1I TTAA 6 cut(s) 1083, 1416, 1550, 2379, 2444, 2643
Tru9I TTAA 6 cut(s) 1083, 1416, 1550, 2379, 2444, 2643
TscAI CASTG 4 cut(s) 350, 917, 1631, 1863
TseFI GTSAC 1 cut(s) 2596
TseI GCWGC 5 cut(s) 92, 730, 1471, 1888, 2289
Tsp45I GTSAC 1 cut(s) 2596
TspDTI ATGAA 6 cut(s) 1085, 1170, 1180, 1351, 1358, 2467
TspGWI ACGGA 4 cut(s) 210, 1016, 1820, 2340
TspRI CASTG 4 cut(s) 350, 917, 1631, 1863
Tth111I GACNNNGTC 1 cut(s) 365
VpaK11BI GGWCC 5 cut(s) 197, 487, 648, 675, 824
XapI RAATTY 6 cut(s) 114, 255, 1076, 1206, 1687, 2542
XbaI TCTAGA 1 cut(s) 1586
XceI RCATGY 1 cut(s) 2195
XcmI CCANNNNNNNNNTGG 2 cut(s) 472, 917
XmiI GTMKAC 1 cut(s) 1242
XspI CTAG 4 cut(s) 771, 1587, 1661, 1781
ZraI GACGTC 2 cut(s) 430, 670
Zsp2I ATGCAT 1 cut(s) 2083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.