Belongs to the glycosyl hydrolase 31 family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Gal_mutarotas_2 | PF13802 | 85 - 249 | 3.3e-11 | Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain |
| Glyco_hydro_31_2nd | PF01055 | 294 - 642 | 1.3e-124 | Glycosyl hydrolases family 31 TIM-barrel domain |
| Glyco_hydro_31_3rd | PF21365 | 651 - 739 | 8.1e-25 | Glycosyl hydrolase family 31 C-terminal domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 126, 789 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1721 |
| AccBSI | CCGCTC | 1 cut(s) | 426 |
| AccI | GTMKAC | 2 cut(s) | 877, 1248 |
| AccII | CGCG | 1 cut(s) | 713 |
| AccIII | TCCGGA | 2 cut(s) | 637, 2147 |
| AclWI | GGATC | 7 cut(s) | 146, 1043, 1127, 1140, 1400, 1477, 2471 |
| AcsI | RAATTY | 7 cut(s) | 270, 1363, 1390, 1693, 1828, 2074, 2441 |
| AcuI | CTGAAG | 2 cut(s) | 2010, 2579 |
| AdeI | CACNNNGTG | 1 cut(s) | 1277 |
| AfaI | GTAC | 6 cut(s) | 548, 860, 1266, 1622, 1673, 2305 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 777, 1223, 1331, 1721, 2487, 2491 |
| AgeI | ACCGGT | 2 cut(s) | 770, 1902 |
| AgsI | TTSAA | 9 cut(s) | 580, 742, 995, 1078, 1693, 1809, 1833, 2122, 2432 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 682 |
| AjiI | CACGTC | 1 cut(s) | 679 |
| AjnI | CCWGG | 2 cut(s) | 1221, 1465 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 467, 499, 560, 592, 2147, 2179 |
| AleI | CACNNNNGTG | 1 cut(s) | 2132 |
| Alw21I | GWGCWC | 5 cut(s) | 870, 1125, 1848, 2398, 2423 |
| Alw26I | GTCTC | 4 cut(s) | 191, 245, 557, 1635 |
| AlwI | GGATC | 7 cut(s) | 146, 1043, 1127, 1140, 1400, 1477, 2471 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 601, 2026 |
| Ama87I | CYCGRG | 2 cut(s) | 157, 1591 |
| Aor13HI | TCCGGA | 2 cut(s) | 637, 2147 |
| AoxI | GGCC | 9 cut(s) | 829, 1235, 1333, 1435, 1551, 1791, 1967, 2217, 2232 |
| ApaI | GGGCCC | 1 cut(s) | 833 |
| ApeKI | GCWGC | 6 cut(s) | 307, 627, 1868, 1894, 2062, 2249 |
| ApoI | RAATTY | 7 cut(s) | 270, 1363, 1390, 1693, 1828, 2074, 2441 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 428, 460, 2392, 2424, 2466, 2498 |
| AsiGI | ACCGGT | 2 cut(s) | 770, 1902 |
| AsuC2I | CCSGG | 3 cut(s) | 1137, 1239, 1683 |
| AsuII | TTCGAA | 2 cut(s) | 274, 2268 |
| AvaI | CYCGRG | 2 cut(s) | 157, 1591 |
| AvaII | GGWCC | 7 cut(s) | 212, 496, 537, 612, 640, 1635, 1953 |
| BaeGI | GKGCMC | 1 cut(s) | 833 |
| BamHI | GGATCC | 1 cut(s) | 1132 |
| BanII | GRGCYC | 4 cut(s) | 833, 870, 1848, 2423 |
| BauI | CACGAG | 1 cut(s) | 2391 |
| BbsI | GAAGAC | 2 cut(s) | 67, 170 |
| Bbv12I | GWGCWC | 5 cut(s) | 870, 1125, 1848, 2398, 2423 |
| BbvI | GCAGC | 6 cut(s) | 294, 639, 1880, 1881, 2074, 2261 |
| BccI | CCATC | 8 cut(s) | 51, 294, 422, 844, 1202, 1325, 2038, 2321 |
| BciT130I | CCWGG | 2 cut(s) | 1223, 1467 |
| BciVI | GTATCC | 2 cut(s) | 995, 1744 |
| BclI | TGATCA | 1 cut(s) | 2179 |
| BcnI | CCSGG | 3 cut(s) | 1137, 1239, 1683 |
| BcoDI | GTCTC | 4 cut(s) | 191, 245, 557, 1635 |
| BfaI | CTAG | 7 cut(s) | 587, 981, 1067, 1173, 1313, 1787, 2411 |
| BfmI | CTRYAG | 1 cut(s) | 2532 |
| BfuI | GTATCC | 2 cut(s) | 995, 1744 |
| BisI | GCNGC | 7 cut(s) | 308, 424, 628, 1869, 1895, 2063, 2250 |
| BlsI | GCNGC | 7 cut(s) | 309, 425, 629, 1870, 1896, 2064, 2251 |
| Bme1390I | CCNGG | 5 cut(s) | 1137, 1223, 1239, 1467, 1683 |
| Bme18I | GGWCC | 7 cut(s) | 212, 496, 537, 612, 640, 1635, 1953 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 682 |
| BmeT110I | CYCGRG | 2 cut(s) | 157, 1591 |
| BmgBI | CACGTC | 1 cut(s) | 679 |
| BmiI | GGNNCC | 7 cut(s) | 718, 831, 1134, 1165, 1792, 1954, 1955 |
| BmrFI | CCNGG | 5 cut(s) | 1137, 1223, 1239, 1467, 1683 |
| BmsI | GCATC | 4 cut(s) | 879, 1015, 1880, 2158 |
| BpiI | GAAGAC | 2 cut(s) | 67, 170 |
| BpmI | CTGGAG | 1 cut(s) | 1452 |
| Bpu14I | TTCGAA | 2 cut(s) | 274, 2268 |
| BpuEI | CTTGAG | 1 cut(s) | 971 |
| BpuMI | CCSGG | 3 cut(s) | 1137, 1239, 1683 |
| BsaAI | YACGTR | 1 cut(s) | 1120 |
| BsaI | GGTCTC | 2 cut(s) | 191, 557 |
| BsaJI | CCNNGG | 5 cut(s) | 156, 181, 711, 1222, 2485 |
| BsaWI | WCCGGW | 6 cut(s) | 637, 770, 1637, 1902, 2147, 2306 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 524, 554, 2322, 2352, 2534, 2564 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 777, 1223, 1331, 1721, 2487, 2491 |
| Bse118I | RCCGGY | 3 cut(s) | 770, 1770, 1902 |
| Bse1I | ACTGG | 5 cut(s) | 916, 1573, 1637, 2111, 2290 |
| Bse3DI | GCAATG | 3 cut(s) | 754, 1249, 2302 |
| BseAI | TCCGGA | 2 cut(s) | 637, 2147 |
| BseBI | CCWGG | 2 cut(s) | 1223, 1467 |
| BseDI | CCNNGG | 5 cut(s) | 156, 181, 711, 1222, 2485 |
| BseGI | GGATG | 7 cut(s) | 504, 763, 1030, 1267, 1530, 2173, 2329 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 777, 1223, 1331, 1721, 2487, 2491 |
| BseMI | GCAATG | 3 cut(s) | 754, 1249, 2302 |
| BseMII | CTCAG | 3 cut(s) | 216, 855, 1833 |
| BseNI | ACTGG | 5 cut(s) | 916, 1573, 1637, 2111, 2290 |
| BseRI | GAGGAG | 3 cut(s) | 190, 418, 2364 |
| BseSI | GKGCMC | 1 cut(s) | 833 |
| BseXI | GCAGC | 6 cut(s) | 294, 639, 1880, 1881, 2074, 2261 |
| BseYI | CCCAGC | 1 cut(s) | 1888 |
| BsgI | GTGCAG | 3 cut(s) | 940, 2373, 2473 |
| Bsh1236I | CGCG | 1 cut(s) | 713 |
| BshFI | GGCC | 9 cut(s) | 831, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234 |
| BshTI | ACCGGT | 2 cut(s) | 770, 1902 |
| BsiHKAI | GWGCWC | 5 cut(s) | 870, 1125, 1848, 2398, 2423 |
| BsiHKCI | CYCGRG | 2 cut(s) | 157, 1591 |
| BslFI | GGGAC | 1 cut(s) | 1966 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 777, 1223, 1331, 1721, 2487, 2491 |
| BsmAI | GTCTC | 4 cut(s) | 191, 245, 557, 1635 |
| BsmFI | GGGAC | 1 cut(s) | 1966 |
| BsnI | GGCC | 9 cut(s) | 831, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234 |
| Bso31I | GGTCTC | 2 cut(s) | 191, 557 |
| BsoBI | CYCGRG | 2 cut(s) | 157, 1591 |
| Bsp119I | TTCGAA | 2 cut(s) | 274, 2268 |
| Bsp120I | GGGCCC | 1 cut(s) | 829 |
| Bsp1286I | GDGCHC | 6 cut(s) | 833, 870, 1125, 1848, 2398, 2423 |
| Bsp13I | TCCGGA | 2 cut(s) | 637, 2147 |
| Bsp1407I | TGTACA | 1 cut(s) | 1264 |
| BspANI | GGCC | 9 cut(s) | 831, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234 |
| BspCNI | CTCAG | 3 cut(s) | 215, 856, 1832 |
| BspEI | TCCGGA | 2 cut(s) | 637, 2147 |
| BspFNI | CGCG | 1 cut(s) | 713 |
| BspLI | GGNNCC | 7 cut(s) | 718, 831, 1134, 1165, 1792, 1954, 1955 |
| BspPI | GGATC | 7 cut(s) | 146, 1043, 1127, 1140, 1400, 1477, 2471 |
| BspT104I | TTCGAA | 2 cut(s) | 274, 2268 |
| BspTNI | GGTCTC | 2 cut(s) | 191, 557 |
| BsrBI | CCGCTC | 1 cut(s) | 426 |
| BsrDI | GCAATG | 3 cut(s) | 754, 1249, 2302 |
| BsrFI | RCCGGY | 3 cut(s) | 770, 1770, 1902 |
| BsrGI | TGTACA | 1 cut(s) | 1264 |
| BsrI | ACTGG | 5 cut(s) | 916, 1573, 1637, 2111, 2290 |
| BssAI | RCCGGY | 3 cut(s) | 770, 1770, 1902 |
| BssECI | CCNNGG | 5 cut(s) | 156, 181, 711, 1222, 2485 |
| BssSI | CACGAG | 1 cut(s) | 2391 |
| BssT1I | CCWWGG | 2 cut(s) | 181, 2485 |
| Bst2BI | CACGAG | 1 cut(s) | 2391 |
| Bst2UI | CCWGG | 2 cut(s) | 1223, 1467 |
| Bst6I | CTCTTC | 2 cut(s) | 520, 2123 |
| BstAUI | TGTACA | 1 cut(s) | 1264 |
| BstBAI | YACGTR | 1 cut(s) | 1120 |
| BstBI | TTCGAA | 2 cut(s) | 274, 2268 |
| BstC8I | GCNNGC | 2 cut(s) | 967, 1772 |
| BstDEI | CTNAG | 5 cut(s) | 202, 266, 864, 1451, 1819 |
| BstDSI | CCRYGG | 1 cut(s) | 711 |
| BstEII | GGTNACC | 1 cut(s) | 684 |
| BstF5I | GGATG | 7 cut(s) | 504, 763, 1030, 1267, 1530, 2173, 2329 |
| BstFNI | CGCG | 1 cut(s) | 713 |
| BstMAI | GTCTC | 4 cut(s) | 191, 245, 557, 1635 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 971, 1874, 1894 |
| BstNI | CCWGG | 2 cut(s) | 1223, 1467 |
| BstNSI | RCATGY | 1 cut(s) | 2198 |
| BstPI | GGTNACC | 1 cut(s) | 684 |
| BstSCI | CCNGG | 5 cut(s) | 1135, 1221, 1237, 1465, 1681 |
| BstSFI | CTRYAG | 1 cut(s) | 2532 |
| BstSLI | GKGCMC | 1 cut(s) | 833 |
| BstUI | CGCG | 1 cut(s) | 713 |
| BstV1I | GCAGC | 6 cut(s) | 294, 639, 1880, 1881, 2074, 2261 |
| BstV2I | GAAGAC | 2 cut(s) | 67, 170 |
| BstX2I | RGATCY | 1 cut(s) | 1132 |
| BstYI | RGATCY | 1 cut(s) | 1132 |
| BsuI | GTATCC | 2 cut(s) | 995, 1744 |
| BsuRI | GGCC | 9 cut(s) | 831, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234 |
| BtgI | CCRYGG | 1 cut(s) | 711 |
| BtrI | CACGTC | 1 cut(s) | 679 |
| BtsCI | GGATG | 7 cut(s) | 504, 763, 1030, 1267, 1530, 2173, 2329 |
| BtsIMutI | CAGTG | 6 cut(s) | 861, 923, 1630, 2139, 2283, 2524 |
| Cac8I | GCNNGC | 2 cut(s) | 967, 1772 |
| CaiI | CAGNNNCTG | 2 cut(s) | 601, 2026 |
| Cfr10I | RCCGGY | 3 cut(s) | 770, 1770, 1902 |
| Cfr42I | CCGCGG | 1 cut(s) | 714 |
| Csp6I | GTAC | 6 cut(s) | 547, 859, 1265, 1621, 1672, 2304 |
| CspAI | ACCGGT | 2 cut(s) | 770, 1902 |
| CviAII | CATG | 5 cut(s) | 673, 725, 754, 1021, 2195 |
| CviQI | GTAC | 6 cut(s) | 547, 859, 1265, 1621, 1672, 2304 |
| DdeI | CTNAG | 5 cut(s) | 202, 266, 864, 1451, 1819 |
| DraIII | CACNNNGTG | 1 cut(s) | 1277 |
| DriI | GACNNNNNGTC | 1 cut(s) | 682 |
| Eam1104I | CTCTTC | 2 cut(s) | 520, 2123 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 682 |
| EarI | CTCTTC | 2 cut(s) | 520, 2123 |
| EciI | GGCGGA | 2 cut(s) | 816, 2595 |
| Ecl136II | GAGCTC | 3 cut(s) | 868, 1846, 2421 |
| Eco130I | CCWWGG | 2 cut(s) | 181, 2485 |
| Eco147I | AGGCCT | 2 cut(s) | 1553, 2234 |
| Eco24I | GRGCYC | 4 cut(s) | 833, 870, 1848, 2423 |
| Eco31I | GGTCTC | 2 cut(s) | 191, 557 |
| Eco47I | GGWCC | 7 cut(s) | 212, 496, 537, 612, 640, 1635, 1953 |
| Eco53kI | GAGCTC | 3 cut(s) | 868, 1846, 2421 |
| Eco57I | CTGAAG | 2 cut(s) | 2010, 2579 |
| Eco88I | CYCGRG | 2 cut(s) | 157, 1591 |
| Eco91I | GGTNACC | 1 cut(s) | 684 |
| EcoICRI | GAGCTC | 3 cut(s) | 868, 1846, 2421 |
| EcoO109I | RGGNCCY | 3 cut(s) | 537, 1791, 1953 |
| EcoO65I | GGTNACC | 1 cut(s) | 684 |
| EcoRI | GAATTC | 4 cut(s) | 270, 1390, 1828, 2441 |
| EcoRII | CCWGG | 2 cut(s) | 1221, 1465 |
| EcoT14I | CCWWGG | 2 cut(s) | 181, 2485 |
| EcoT38I | GRGCYC | 4 cut(s) | 833, 870, 1848, 2423 |
| ErhI | CCWWGG | 2 cut(s) | 181, 2485 |
| FaeI | CATG | 5 cut(s) | 676, 728, 757, 1024, 2198 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 560, 592 |
| FaqI | GGGAC | 1 cut(s) | 1966 |
| FatI | CATG | 5 cut(s) | 672, 724, 753, 1020, 2194 |
| FauI | CCCGC | 4 cut(s) | 335, 428, 706, 889 |
| FbaI | TGATCA | 1 cut(s) | 2179 |
| FblI | GTMKAC | 2 cut(s) | 877, 1248 |
| Fnu4HI | GCNGC | 7 cut(s) | 308, 424, 628, 1869, 1895, 2063, 2250 |
| FokI | GGATG | 7 cut(s) | 491, 770, 1037, 1254, 1537, 2180, 2336 |
| FriOI | GRGCYC | 4 cut(s) | 833, 870, 1848, 2423 |
| Fsp4HI | GCNGC | 7 cut(s) | 308, 424, 628, 1869, 1895, 2063, 2250 |
| FspBI | CTAG | 7 cut(s) | 587, 981, 1067, 1173, 1313, 1787, 2411 |
| GluI | GCNGC | 7 cut(s) | 308, 424, 628, 1869, 1895, 2063, 2250 |
| GsaI | CCCAGC | 1 cut(s) | 1892 |
| GsuI | CTGGAG | 1 cut(s) | 1452 |
| HaeIII | GGCC | 9 cut(s) | 831, 1237, 1335, 1437, 1553, 1793, 1969, 2219, 2234 |
| Hin1II | CATG | 5 cut(s) | 676, 728, 757, 1024, 2198 |
| HincII | GTYRAC | 3 cut(s) | 178, 878, 1057 |
| HindII | GTYRAC | 3 cut(s) | 178, 878, 1057 |
| HpaI | GTTAAC | 1 cut(s) | 178 |
| Hpy99I | CGWCG | 1 cut(s) | 694 |
| HpyAV | CCTTC | 6 cut(s) | 493, 676, 1106, 1174, 1876, 2198 |
| HpyCH4IV | ACGT | 4 cut(s) | 678, 1119, 2162, 2190 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 971, 1874, 1894 |
| HpyF3I | CTNAG | 5 cut(s) | 202, 266, 864, 1451, 1819 |
| HpySE526I | ACGT | 4 cut(s) | 678, 1119, 2162, 2190 |
| Hsp92II | CATG | 5 cut(s) | 676, 728, 757, 1024, 2198 |
| KflI | GGGWCCC | 1 cut(s) | 1953 |
| Kpn2I | TCCGGA | 2 cut(s) | 637, 2147 |
| KroI | GCCGGC | 1 cut(s) | 1770 |
| KroNI | GCCGGC | 1 cut(s) | 1772 |
| Ksp22I | TGATCA | 1 cut(s) | 2179 |
| KspAI | GTTAAC | 1 cut(s) | 178 |
| KspI | CCGCGG | 1 cut(s) | 714 |
| LmnI | GCTCC | 8 cut(s) | 203, 304, 431, 588, 853, 1724, 2069, 2554 |
| Lsp1109I | GCAGC | 6 cut(s) | 294, 639, 1880, 1881, 2074, 2261 |
| LweI | GCATC | 4 cut(s) | 879, 1015, 1880, 2158 |
| MaeI | CTAG | 7 cut(s) | 587, 981, 1067, 1173, 1313, 1787, 2411 |
| MaeII | ACGT | 4 cut(s) | 678, 1119, 2162, 2190 |
| MbiI | CCGCTC | 1 cut(s) | 426 |
| MfeI | CAATTG | 1 cut(s) | 1959 |
| MflI | RGATCY | 1 cut(s) | 1132 |
| MhlI | GDGCHC | 6 cut(s) | 833, 870, 1125, 1848, 2398, 2423 |
| MlyI | GAGTC | 4 cut(s) | 143, 278, 592, 1695 |
| MmeI | TCCRAC | 7 cut(s) | 492, 1702, 1757, 2145, 2441, 2472, 2532 |
| MroI | TCCGGA | 2 cut(s) | 637, 2147 |
| MroNI | GCCGGC | 1 cut(s) | 1770 |
| MseI | TTAA | 4 cut(s) | 177, 492, 1302, 2502 |
| MslI | CAYNNNNRTG | 4 cut(s) | 677, 1928, 2132, 2189 |
| MspA1I | CMGCKG | 1 cut(s) | 713 |
| MspR9I | CCNGG | 5 cut(s) | 1137, 1223, 1239, 1467, 1683 |
| MunI | CAATTG | 1 cut(s) | 1959 |
| MvaI | CCWGG | 2 cut(s) | 1223, 1467 |
| MvnI | CGCG | 1 cut(s) | 713 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 971, 1874, 1894 |
| NaeI | GCCGGC | 1 cut(s) | 1772 |
| NciI | CCSGG | 3 cut(s) | 1137, 1239, 1683 |
| NgoMIV | GCCGGC | 1 cut(s) | 1770 |
| NlaIII | CATG | 5 cut(s) | 676, 728, 757, 1024, 2198 |
| NlaIV | GGNNCC | 7 cut(s) | 718, 831, 1134, 1165, 1792, 1954, 1955 |
| NmuCI | GTSAC | 5 cut(s) | 358, 684, 695, 773, 2281 |
| NspI | RCATGY | 1 cut(s) | 2198 |
| NspV | TTCGAA | 2 cut(s) | 274, 2268 |
| OliI | CACNNNNGTG | 1 cut(s) | 2132 |
| PaeR7I | CTCGAG | 1 cut(s) | 1591 |
| PceI | AGGCCT | 2 cut(s) | 1553, 2234 |
| PdiI | GCCGGC | 1 cut(s) | 1772 |
| PfeI | GAWTC | 6 cut(s) | 634, 909, 1780, 1859, 2144, 2566 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1721 |
| PfoI | TCCNGGA | 1 cut(s) | 1465 |
| PinAI | ACCGGT | 2 cut(s) | 770, 1902 |
| PkrI | GCNGC | 7 cut(s) | 309, 425, 629, 1870, 1896, 2064, 2251 |
| PleI | GAGTC | 4 cut(s) | 142, 278, 592, 1695 |
| PpsI | GAGTC | 4 cut(s) | 142, 278, 592, 1695 |
| Ppu21I | YACGTR | 1 cut(s) | 1120 |
| PpuMI | RGGWCCY | 2 cut(s) | 537, 1953 |
| PsiI | TTATAA | 2 cut(s) | 126, 789 |
| Psp124BI | GAGCTC | 3 cut(s) | 870, 1848, 2423 |
| Psp5II | RGGWCCY | 2 cut(s) | 537, 1953 |
| Psp6I | CCWGG | 2 cut(s) | 1221, 1465 |
| PspEI | GGTNACC | 1 cut(s) | 684 |
| PspFI | CCCAGC | 1 cut(s) | 1888 |
| PspGI | CCWGG | 2 cut(s) | 1221, 1465 |
| PspN4I | GGNNCC | 7 cut(s) | 718, 831, 1134, 1165, 1792, 1954, 1955 |
| PspOMI | GGGCCC | 1 cut(s) | 829 |
| PspPPI | RGGWCCY | 2 cut(s) | 537, 1953 |
| PstNI | CAGNNNCTG | 2 cut(s) | 601, 2026 |
| PsuI | RGATCY | 1 cut(s) | 1132 |
| RsaI | GTAC | 6 cut(s) | 548, 860, 1266, 1622, 1673, 2305 |
| RsaNI | GTAC | 6 cut(s) | 547, 859, 1265, 1621, 1672, 2304 |
| RseI | CAYNNNNRTG | 4 cut(s) | 677, 1928, 2132, 2189 |
| SacI | GAGCTC | 3 cut(s) | 870, 1848, 2423 |
| SacII | CCGCGG | 1 cut(s) | 714 |
| SalI | GTCGAC | 1 cut(s) | 876 |
| SaqAI | TTAA | 4 cut(s) | 177, 492, 1302, 2502 |
| SatI | GCNGC | 7 cut(s) | 308, 424, 628, 1869, 1895, 2063, 2250 |
| SchI | GAGTC | 4 cut(s) | 143, 278, 592, 1695 |
| ScrFI | CCNGG | 5 cut(s) | 1137, 1223, 1239, 1467, 1683 |
| SduI | GDGCHC | 6 cut(s) | 833, 870, 1125, 1848, 2398, 2423 |
| SfaNI | GCATC | 4 cut(s) | 879, 1015, 1880, 2158 |
| SfcI | CTRYAG | 1 cut(s) | 2532 |
| Sfr274I | CTCGAG | 1 cut(s) | 1591 |
| Sfr303I | CCGCGG | 1 cut(s) | 714 |
| SfuI | TTCGAA | 2 cut(s) | 274, 2268 |
| SgrAI | CRCCGGYG | 1 cut(s) | 770 |
| SgrBI | CCGCGG | 1 cut(s) | 714 |
| SinI | GGWCC | 7 cut(s) | 212, 496, 537, 612, 640, 1635, 1953 |
| SlaI | CTCGAG | 1 cut(s) | 1591 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 677, 1928, 2132, 2189 |
| SmlI | CTYRAG | 2 cut(s) | 950, 1591 |
| SmoI | CTYRAG | 2 cut(s) | 950, 1591 |
| SseBI | AGGCCT | 2 cut(s) | 1553, 2234 |
| SspMI | CTAG | 7 cut(s) | 587, 981, 1067, 1173, 1313, 1787, 2411 |
| SstI | GAGCTC | 3 cut(s) | 870, 1848, 2423 |
| StuI | AGGCCT | 2 cut(s) | 1553, 2234 |
| StyD4I | CCNGG | 5 cut(s) | 1135, 1221, 1237, 1465, 1681 |
| StyI | CCWWGG | 2 cut(s) | 181, 2485 |
| TaiI | ACGT | 4 cut(s) | 681, 1122, 2165, 2193 |
| TaqI | TCGA | 8 cut(s) | 132, 274, 877, 1592, 1598, 2112, 2268, 2340 |
| TatI | WGTACW | 3 cut(s) | 858, 1264, 1620 |
| TauI | GCSGC | 1 cut(s) | 426 |
| TfiI | GAWTC | 6 cut(s) | 634, 909, 1780, 1859, 2144, 2566 |
| Tru1I | TTAA | 4 cut(s) | 177, 492, 1302, 2502 |
| Tru9I | TTAA | 4 cut(s) | 177, 492, 1302, 2502 |
| TscAI | CASTG | 6 cut(s) | 868, 923, 1637, 2139, 2290, 2524 |
| TseFI | GTSAC | 5 cut(s) | 358, 684, 695, 773, 2281 |
| TseI | GCWGC | 6 cut(s) | 307, 627, 1868, 1894, 2062, 2249 |
| Tsp45I | GTSAC | 5 cut(s) | 358, 684, 695, 773, 2281 |
| TspDTI | ATGAA | 7 cut(s) | 475, 1186, 1364, 1368, 1499, 1773, 2372 |
| TspGWI | ACGGA | 4 cut(s) | 296, 346, 714, 1022 |
| TspRI | CASTG | 6 cut(s) | 868, 923, 1637, 2139, 2290, 2524 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1721 |
| VpaK11BI | GGWCC | 7 cut(s) | 212, 496, 537, 612, 640, 1635, 1953 |
| XapI | RAATTY | 7 cut(s) | 270, 1363, 1390, 1693, 1828, 2074, 2441 |
| XbaI | TCTAGA | 1 cut(s) | 1312 |
| XceI | RCATGY | 1 cut(s) | 2198 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 923 |
| XhoI | CTCGAG | 1 cut(s) | 1591 |
| XmiI | GTMKAC | 2 cut(s) | 877, 1248 |
| XspI | CTAG | 7 cut(s) | 587, 981, 1067, 1173, 1313, 1787, 2411 |