FvH4_4g06480

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000839
Physical Location & Seq
Forward (+)
5766151 .. 5770300
4150 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06480.t1

Sequence Viewer

Length: 1497 bp
ATGTCTCTTCTGCAACTATATATAAACCAATTTGAAGATATGGAGTTTGTTTCTCATTTGGTGCCCACAATGACAATGACGGGTTTTCTTCTTCATGTAATGTTGGTCTTCACTTTGATACTCACTTCAACTTCAGCTGCCAACATCACTACAACCAACGTTACCAGTCTACCGGGTTATTATTCCGGTGACCTTCCCTTCGCGCTAGAATCGGGATATATCGGTATAGGTGACAATGAAGAAGTGCAGATATTCTACCTTTTCGTTGAGTCTGAGGGAGCTCCCGCAGACGATCCTTTGTTGCTATGGTTCAATGGAGGCCCAGGTTGCTCTGGTCTTGCTGGATTTTTCTTTGAGAGTGGTCCAGTAACTTTCAAGTTTGGAAATTACAATGGGAGCCTACCAGCAGTTCTAGACAATCCATTTCGATGGACAAAGGACGTCAACATGATATACATAGATGCACCTGTTGGTGCTGGCTTCTCCTACTCGAAAACTCAAAGCGGTTATGTTATGGATGACTACAAATATGTTGCACAGAGCTATGAATTTCTGCATAAGTGGCTGGAGGCACACCCTGCATATTTGGAAAATGTGCTCTACATAGGTGGTGACTCCTTCTCTGGCATAATTGTGCCAATGATTGTGCAACAAATATTGGATGGTAATGAAAATGGAACTTTGCCTCTAATGAATCTCAAGGGATATGTAATTGGGAACCCAGTAACAGATACAGTCATTGATACAAATGCAAGAATTCCATATGCCCATCGGCTAACTCTTATATCAGATCAACTGTATCAGGCGGCGAGAACAAGTTGCAGTGGAGATTACGTAAACGTAAATTCAAGCAATGATGCATGCATTTCAGATATTGATGCTATTGATGCACTCACAAATGATATAAATATGTTACACATCTTGGAACCAGTCTGTAGCGATTCATTGCCAAGACCAAAGGAGAAGGAGATAGGTACAGCCCGAAGATCTCTGAATGATTTTCCCGAAGGTTTACTAAGCTCAGCAACCAATGGACCTGCATATTGGTGTAGGAGTTATAATCATATGCTAGCCTACATTTGGGCAAACCACCCGAGTGTCCAAGATGCTCTAGGTGTCCGAAGGGGCACGAAAAGATTTTGGCAATACTGTAATTCAACCCTGGCATACACTCAAGAAGTCACTAGTGTTATTAGTCTTCATCAAAGTCTCGCGACAGTTGGCCTACGTGCTCTGATATACAGTGGCGATCACGACATGACAATTCCCCACATTGCTACACAAAAGTGGATAAACTTGCTCAATTTGACTCTGAATGAGACCTGGAGGGCATGGTCAGTTGATAACCAAACTGCAGGGTACACCAAGAAGTTTGTTAATGAAGTTTTCAATTTGACGTTTGCGACTGTAAAGGGAGGAGGCCACATTGCTGCAGAGTACAAAGTCAAAGAATGCTCTGAAATGATTGATAGATGGATGGCTCAAACCCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

499

Amino Acids

55.36

Weight (kDa)

4.87

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 63 - 494 3.1e-115 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
AatII GACGTC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 1045
AccB1I GGYRCC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 169
AccII CGCG 2 cut(s) 203, 1214
AciI CCGC 3 cut(s) 285, 504, 806
AclI AACGTT 1 cut(s) 159
AclWI GGATC 1 cut(s) 287
AcsI RAATTY 3 cut(s) 548, 756, 844
AcuI CTGAAG 1 cut(s) 117
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 3 cut(s) 976, 1361, 1439
AfiI CCNNNNNNNGG 1 cut(s) 1080
AgsI TTSAA 7 cut(s) 35, 129, 313, 376, 849, 1158, 1390
AhlI ACTAGT 1 cut(s) 1184
AjnI CCWGG 3 cut(s) 322, 1161, 1322
AleI CACNNNNGTG 2 cut(s) 1095, 1285
AluBI AGCT 4 cut(s) 137, 281, 543, 1020
AluI AGCT 4 cut(s) 137, 281, 543, 1020
Alw21I GWGCWC 3 cut(s) 283, 600, 1234
Alw26I GTCTC 3 cut(s) 9, 1214, 1313
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 1093
AoxI GGCC 3 cut(s) 319, 1222, 1420
ApeKI GCWGC 2 cut(s) 137, 1430
ApoI RAATTY 3 cut(s) 548, 756, 844
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 3 cut(s) 320, 362, 1034
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 3 cut(s) 200, 242, 623
AsuNHI GCTAGC 1 cut(s) 1069
AvaI CYCGRG 1 cut(s) 1093
AvaII GGWCC 2 cut(s) 362, 1034
BaeGI GKGCMC 2 cut(s) 66, 1130
BanI GGYRCC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 283
BbsI GAAGAC 2 cut(s) 100, 1190
Bbv12I GWGCWC 3 cut(s) 283, 600, 1234
BbvI GCAGC 2 cut(s) 124, 1417
BccI CCATC 5 cut(s) 423, 656, 777, 1467, 1471
BciT130I CCWGG 3 cut(s) 324, 1163, 1324
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 3 cut(s) 9, 1214, 1313
BcuI ACTAGT 1 cut(s) 1184
BfaI CTAG 5 cut(s) 206, 413, 1070, 1112, 1185
BfmI CTRYAG 3 cut(s) 934, 1353, 1431
BfuAI ACCTGC 1 cut(s) 1045
BglII AGATCT 1 cut(s) 986
BisI GCNGC 3 cut(s) 138, 807, 1431
BlpI GCTNAGC 1 cut(s) 1021
BlsI GCNGC 3 cut(s) 139, 808, 1432
Bme1390I CCNGG 4 cut(s) 174, 324, 1163, 1324
Bme18I GGWCC 2 cut(s) 362, 1034
BmeT110I CYCGRG 1 cut(s) 1093
BmgT120I GGNCC 3 cut(s) 320, 362, 1034
BmiI GGNNCC 4 cut(s) 63, 398, 719, 927
BmrFI CCNGG 4 cut(s) 174, 324, 1163, 1324
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 5 cut(s) 451, 847, 868, 877, 1096
BmtI GCTAGC 1 cut(s) 1073
BmuI ACTGGG 1 cut(s) 716
BpiI GAAGAC 2 cut(s) 100, 1190
BpmI CTGGAG 2 cut(s) 587, 1345
Bpu1102I GCTNAGC 1 cut(s) 1021
BpuEI CTTGAG 2 cut(s) 683, 1158
BpuMI CCSGG 1 cut(s) 174
BsaAI YACGTR 2 cut(s) 835, 1229
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 1313
BsaJI CCNNGG 2 cut(s) 322, 1161
BsaWI WCCGGW 1 cut(s) 185
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 1080
Bse1I ACTGG 4 cut(s) 165, 365, 722, 929
Bse3DI GCAATG 4 cut(s) 859, 944, 1272, 1425
BseBI CCWGG 3 cut(s) 324, 1163, 1324
BseDI CCNNGG 2 cut(s) 322, 1161
BseGI GGATG 3 cut(s) 523, 667, 1482
BseLI CCNNNNNNNGG 1 cut(s) 1080
BseMI GCAATG 4 cut(s) 859, 944, 1272, 1425
BseMII CTCAG 2 cut(s) 264, 1035
BseNI ACTGG 4 cut(s) 165, 365, 722, 929
BseRI GAGGAG 1 cut(s) 1431
BseSI GKGCMC 2 cut(s) 66, 1130
BseXI GCAGC 2 cut(s) 124, 1417
BsgI GTGCAG 1 cut(s) 266
Bsh1236I CGCG 2 cut(s) 203, 1214
BshFI GGCC 3 cut(s) 321, 1224, 1422
BshNI GGYRCC 1 cut(s) 61
BsiHKAI GWGCWC 3 cut(s) 283, 600, 1234
BsiHKCI CYCGRG 1 cut(s) 1093
BsiSI CCGG 2 cut(s) 173, 186
BslI CCNNNNNNNGG 1 cut(s) 1080
BsmAI GTCTC 3 cut(s) 9, 1214, 1313
BsmI GAATGC 1 cut(s) 1457
BsnI GGCC 3 cut(s) 321, 1224, 1422
Bso31I GGTCTC 1 cut(s) 1313
BsoBI CYCGRG 1 cut(s) 1093
Bsp1286I GDGCHC 5 cut(s) 66, 283, 600, 1130, 1234
Bsp143I GATC 4 cut(s) 292, 790, 986, 1249
Bsp1720I GCTNAGC 1 cut(s) 1021
Bsp68I TCGCGA 1 cut(s) 1214
BspACI CCGC 3 cut(s) 285, 504, 806
BspANI GGCC 3 cut(s) 321, 1224, 1422
BspCNI CTCAG 2 cut(s) 265, 1034
BspFNI CGCG 2 cut(s) 203, 1214
BspLI GGNNCC 4 cut(s) 63, 398, 719, 927
BspMAI CTGCAG 2 cut(s) 1357, 1435
BspMI ACCTGC 1 cut(s) 1045
BspOI GCTAGC 1 cut(s) 1073
BspPI GGATC 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 61
BspTNI GGTCTC 1 cut(s) 1313
BsrDI GCAATG 4 cut(s) 859, 944, 1272, 1425
BsrI ACTGG 4 cut(s) 165, 365, 722, 929
BssECI CCNNGG 2 cut(s) 322, 1161
BssMI GATC 4 cut(s) 292, 790, 986, 1249
BssNI GRCGYC 1 cut(s) 441
Bst2UI CCWGG 3 cut(s) 324, 1163, 1324
Bst4CI ACNGT 6 cut(s) 736, 798, 1151, 1219, 1244, 1408
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 441
BstAPI GCANNNNNTGC 1 cut(s) 578
BstBAI YACGTR 2 cut(s) 835, 1229
BstC8I GCNNGC 3 cut(s) 478, 862, 1071
BstDEI CTNAG 3 cut(s) 273, 1016, 1021
BstEII GGTNACC 1 cut(s) 188
BstF5I GGATG 3 cut(s) 523, 667, 1482
BstFNI CGCG 2 cut(s) 203, 1214
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 4 cut(s) 295, 793, 989, 1252
BstMAI GTCTC 3 cut(s) 9, 1214, 1313
BstMBI GATC 4 cut(s) 292, 790, 986, 1249
BstMWI GCNNNNNNNGC 4 cut(s) 327, 562, 578, 887
BstNI CCWGG 3 cut(s) 324, 1163, 1324
BstNSI RCATGY 1 cut(s) 864
BstPI GGTNACC 1 cut(s) 188
BstSCI CCNGG 4 cut(s) 172, 322, 1161, 1322
BstSFI CTRYAG 3 cut(s) 934, 1353, 1431
BstSLI GKGCMC 2 cut(s) 66, 1130
BstSNI TACGTA 1 cut(s) 835
BstUI CGCG 2 cut(s) 203, 1214
BstV1I GCAGC 2 cut(s) 124, 1417
BstV2I GAAGAC 2 cut(s) 100, 1190
BstX2I RGATCY 1 cut(s) 986
BstXI CCANNNNNNTGG 1 cut(s) 429
BstYI RGATCY 1 cut(s) 986
BsuRI GGCC 3 cut(s) 321, 1224, 1422
BtsCI GGATG 3 cut(s) 523, 667, 1482
BtsI GCAGTG 1 cut(s) 829
BtsIMutI CAGTG 2 cut(s) 829, 1249
BtuMI TCGCGA 1 cut(s) 1214
BveI ACCTGC 1 cut(s) 1045
Cac8I GCNNGC 3 cut(s) 478, 862, 1071
CfoI GCGC 1 cut(s) 205
Cfr13I GGNCC 3 cut(s) 320, 362, 1034
Csp6I GTAC 3 cut(s) 975, 1360, 1438
CviAII CATG 5 cut(s) 95, 448, 861, 1258, 1332
CviQI GTAC 3 cut(s) 975, 1360, 1438
DdeI CTNAG 3 cut(s) 273, 1016, 1021
DpnI GATC 4 cut(s) 294, 792, 988, 1251
DpnII GATC 4 cut(s) 292, 790, 986, 1249
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 281
Eco105I TACGTA 1 cut(s) 835
Eco24I GRGCYC 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 1313
Eco47I GGWCC 2 cut(s) 362, 1034
Eco53kI GAGCTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 1093
Eco91I GGTNACC 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 281
EcoO65I GGTNACC 1 cut(s) 188
EcoRI GAATTC 1 cut(s) 756
EcoRII CCWGG 3 cut(s) 322, 1161, 1322
EcoT22I ATGCAT 2 cut(s) 862, 866
EcoT38I GRGCYC 1 cut(s) 283
FaeI CATG 5 cut(s) 98, 451, 864, 1261, 1335
FatI CATG 5 cut(s) 94, 447, 860, 1257, 1331
FauI CCCGC 1 cut(s) 292
FauNDI CATATG 2 cut(s) 763, 1065
FblI GTMKAC 1 cut(s) 169
Fnu4HI GCNGC 3 cut(s) 138, 807, 1431
FokI GGATG 3 cut(s) 530, 674, 1489
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 3 cut(s) 138, 807, 1431
FspBI CTAG 5 cut(s) 206, 413, 1070, 1112, 1185
GlaI GCGC 1 cut(s) 204
GluI GCNGC 3 cut(s) 138, 807, 1431
GsuI CTGGAG 2 cut(s) 587, 1345
HaeIII GGCC 3 cut(s) 321, 1224, 1422
HapII CCGG 2 cut(s) 173, 186
HhaI GCGC 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 5 cut(s) 98, 451, 864, 1261, 1335
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HinfI GANTC 6 cut(s) 209, 269, 614, 694, 941, 1309
HpaII CCGG 2 cut(s) 173, 186
HphI GGTGA 3 cut(s) 200, 242, 623
Hpy166II GTNNAC 5 cut(s) 170, 445, 838, 1013, 1362
Hpy188I TCNGA 9 cut(s) 274, 790, 871, 993, 1121, 1236, 1314, 1459, 1496
Hpy188III TCNNGA 6 cut(s) 213, 413, 1004, 1175, 1213, 1253
Hpy8I GTNNAC 5 cut(s) 170, 445, 838, 1013, 1362
HpyAV CCTTC 6 cut(s) 203, 208, 628, 958, 1001, 1116
HpyCH4III ACNGT 6 cut(s) 736, 798, 1151, 1219, 1244, 1408
HpyCH4IV ACGT 6 cut(s) 159, 441, 834, 840, 1228, 1397
HpyF10VI GCNNNNNNNGC 4 cut(s) 327, 562, 578, 887
HpyF3I CTNAG 3 cut(s) 273, 1016, 1021
HpySE526I ACGT 6 cut(s) 159, 441, 834, 840, 1228, 1397
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 5 cut(s) 98, 451, 864, 1261, 1335
HspAI GCGC 1 cut(s) 203
Kzo9I GATC 4 cut(s) 292, 790, 986, 1249
LmnI GCTCC 3 cut(s) 278, 286, 396
Lsp1109I GCAGC 2 cut(s) 124, 1417
LweI GCATC 5 cut(s) 451, 847, 868, 877, 1096
MaeI CTAG 5 cut(s) 206, 413, 1070, 1112, 1185
MaeII ACGT 6 cut(s) 159, 441, 834, 840, 1228, 1397
MaeIII GTNAC 8 cut(s) 160, 188, 230, 367, 611, 724, 912, 1180
MalI GATC 4 cut(s) 294, 792, 988, 1251
MboI GATC 4 cut(s) 292, 790, 986, 1249
MboII GAAGA 7 cut(s) 47, 80, 83, 100, 251, 996, 1190
MflI RGATCY 1 cut(s) 986
MhlI GDGCHC 5 cut(s) 66, 283, 600, 1130, 1234
MlyI GAGTC 3 cut(s) 278, 608, 1303
MnlI CCTC 7 cut(s) 268, 311, 562, 696, 1320, 1409, 1412
Mph1103I ATGCAT 2 cut(s) 862, 866
MseI TTAA 1 cut(s) 1377
MslI CAYNNNNRTG 5 cut(s) 427, 632, 1045, 1095, 1285
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 2 cut(s) 173, 186
MspR9I CCNGG 4 cut(s) 174, 324, 1163, 1324
Mva1269I GAATGC 1 cut(s) 1457
MvaI CCWGG 3 cut(s) 324, 1163, 1324
MvnI CGCG 2 cut(s) 203, 1214
MwoI GCNNNNNNNGC 4 cut(s) 327, 562, 578, 887
NciI CCSGG 1 cut(s) 174
NdeI CATATG 2 cut(s) 763, 1065
NdeII GATC 4 cut(s) 292, 790, 986, 1249
NheI GCTAGC 1 cut(s) 1069
NlaIII CATG 5 cut(s) 98, 451, 864, 1261, 1335
NlaIV GGNNCC 4 cut(s) 63, 398, 719, 927
NmuCI GTSAC 4 cut(s) 188, 230, 611, 1180
NruI TCGCGA 1 cut(s) 1214
NsiI ATGCAT 2 cut(s) 862, 866
NspI RCATGY 1 cut(s) 864
OliI CACNNNNGTG 2 cut(s) 1095, 1285
PaeI GCATGC 1 cut(s) 864
PctI GAATGC 1 cut(s) 1457
PfeI GAWTC 3 cut(s) 209, 694, 941
PkrI GCNGC 3 cut(s) 139, 808, 1432
PleI GAGTC 3 cut(s) 277, 608, 1303
PpsI GAGTC 3 cut(s) 277, 608, 1303
Ppu21I YACGTR 2 cut(s) 835, 1229
PsiI TTATAA 1 cut(s) 1059
Psp124BI GAGCTC 1 cut(s) 283
Psp1406I AACGTT 1 cut(s) 159
Psp6I CCWGG 3 cut(s) 322, 1161, 1322
PspEI GGTNACC 1 cut(s) 188
PspGI CCWGG 3 cut(s) 322, 1161, 1322
PspN4I GGNNCC 4 cut(s) 63, 398, 719, 927
PspPI GGNCC 3 cut(s) 320, 362, 1034
PstI CTGCAG 2 cut(s) 1357, 1435
PsuI RGATCY 1 cut(s) 986
PvuII CAGCTG 1 cut(s) 137
RruI TCGCGA 1 cut(s) 1214
RsaI GTAC 3 cut(s) 976, 1361, 1439
RsaNI GTAC 3 cut(s) 975, 1360, 1438
RseI CAYNNNNRTG 5 cut(s) 427, 632, 1045, 1095, 1285
SacI GAGCTC 1 cut(s) 283
SaqAI TTAA 1 cut(s) 1377
SatI GCNGC 3 cut(s) 138, 807, 1431
Sau3AI GATC 4 cut(s) 292, 790, 986, 1249
Sau96I GGNCC 3 cut(s) 320, 362, 1034
SchI GAGTC 3 cut(s) 278, 608, 1303
ScrFI CCNGG 4 cut(s) 174, 324, 1163, 1324
SduI GDGCHC 5 cut(s) 66, 283, 600, 1130, 1234
SfaNI GCATC 5 cut(s) 451, 847, 868, 877, 1096
SfcI CTRYAG 3 cut(s) 934, 1353, 1431
SinI GGWCC 2 cut(s) 362, 1034
SmiMI CAYNNNNRTG 5 cut(s) 427, 632, 1045, 1095, 1285
SmlI CTYRAG 2 cut(s) 698, 1173
SmoI CTYRAG 2 cut(s) 698, 1173
SnaBI TACGTA 1 cut(s) 835
SpeI ACTAGT 1 cut(s) 1184
SphI GCATGC 1 cut(s) 864
SsiI CCGC 3 cut(s) 285, 504, 806
SspI AATATT 1 cut(s) 657
SspMI CTAG 5 cut(s) 206, 413, 1070, 1112, 1185
SstI GAGCTC 1 cut(s) 283
StyD4I CCNGG 4 cut(s) 172, 322, 1161, 1322
TaaI ACNGT 6 cut(s) 736, 798, 1151, 1219, 1244, 1408
TaiI ACGT 6 cut(s) 162, 444, 837, 843, 1231, 1400
TaqI TCGA 2 cut(s) 427, 491
TatI WGTACW 1 cut(s) 1437
TauI GCSGC 1 cut(s) 809
TfiI GAWTC 3 cut(s) 209, 694, 941
Tru1I TTAA 1 cut(s) 1377
Tru9I TTAA 1 cut(s) 1377
TscAI CASTG 2 cut(s) 829, 1249
TseFI GTSAC 4 cut(s) 188, 230, 611, 1180
TseI GCWGC 2 cut(s) 137, 1430
Tsp45I GTSAC 4 cut(s) 188, 230, 611, 1180
TspDTI ATGAA 8 cut(s) 83, 252, 561, 684, 707, 933, 1190, 1395
TspRI CASTG 2 cut(s) 829, 1249
VpaK11BI GGWCC 2 cut(s) 362, 1034
XapI RAATTY 3 cut(s) 548, 756, 844
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 1 cut(s) 864
XmiI GTMKAC 1 cut(s) 169
XspI CTAG 5 cut(s) 206, 413, 1070, 1112, 1185
ZraI GACGTC 1 cut(s) 442
Zsp2I ATGCAT 2 cut(s) 862, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.