Rroxscaffold_5G00343260

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000839
Physical Location & Seq
Forward (+)
12552828 .. 12556955
4128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343260.1

Sequence Viewer

Length: 1458 bp
ATGGATTTTGTTCATCATTTGGTACCCACAATGAAAATGATGGATTTTCTTCTACTGTTAATGTTGGCTTTCACTTCGATACTCACTTCAACTTCAGCTGCCAGCATTACTACAACCAACATTACGATTCTACCGGGTTATTCCGGTGACCTTCCTTTTGCACTGGAATCCGGATATATCGGTGTAGGTGACAATGAAGAAGTGCAGCTATTTTATGTTTTCGTTGAGTCTGAGAGAGCTCCTGCAGACGATCCTCTGTTGCTATGGTTCAATGGAGGCCCAGGTTGCTCTGGTCTTGCTGGATTCTTCTTTGAGAGTGGTCCAGTAACTTTTAAGTCTGAAAATTACAATGGGAGTCTCCCAGCAGTTCTTGACAATCCATTTCGATGGACAAAGGACGTCAGCATGATATACATAGATGCACCTGTTGGTGCTGGATTCTCCTACTCAAAAACTCAAAGCGGTTATGTTATGGATGACTATAAATATGTTGCACAAAGCTATGAATTTCTGCAGAAGTGGCTGGACGCACACCCTGCATATTTGGAAAATTTGCTCTACATTGGTGGTGACTCCTTCTCTGGAATAATTGTGCCAATGATTGTACAACAAATATTGGATGGTATTGAAAACGCAACTTTGCCTCTAATGAATCTCAAGGGATATGTAATTGGGAACCCAGTAACGGATTCATTCATTGATGCGAATGCAAGAATTCCTTATGCCCATCGGCTAACTCTTATATCAGATCAACTGTATCAGGCGGCGAAAACAAGTTGCAGTGGAGATTACGTAAACGTAGATTCAAGCAATGACGCATGCATTTCAGACATCGATGCTATTGATGCACTCATAAATGACGTAAATACCTTACACGTCTTGGAACCAGTGTGTAGTGAAGGCACTGCCCGAAGATATCTCAAGGATTTGTCCGAAGGTTTACTAAGTTCAGCAACTACTGGTCCTGCATATTGGTGTAGGAGTTATAACCATATGCTCGCCTACAATTGGGCAAATCACCAAAGTGTTCAAGATGCTCTTGGCGTCCGAAGGGGCACAAAAAGATTTTGGCAATACTGTAATTCAACCCTGGCGTACACTCAAGAGGTCACTAGTGTTATTAGTCTTCATCAAAGTTTCGCGGATGTTGGCCTACGTGCTCTGATCTACAGTGGTGATCACGACATGACAATTCCTCACATTGCTACACAAAAATGGATAAACTTTCTCAATTTGACTCTGAATGAGGCCTGGAGGGCATGGTCAGTTGATAACCAAACTGCAGGGTACACAAAGAAGTATGCAAATGTAGCTTTCAATTTGACGTTTGCGACTATAAAGGCAAGCATAACTTTTTACTTTGCTCTCCCTCTGGGAGGAGGCCACATTGCTGCAGAGTACAAGGTCAAAGAATGCTCTGCAATGATTGATAGATGGATGGCTCAGTACCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

485

Amino Acids

53.81

Weight (kDa)

4.87

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 45 - 481 3.7e-110 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 987
AatII GACGTC 1 cut(s) 402
Acc65I GGTACC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 22
AccII CGCG 1 cut(s) 1142
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 3 cut(s) 462, 764, 1142
AclWI GGATC 1 cut(s) 245
AcsI RAATTY 3 cut(s) 506, 550, 714
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 399, 1044
AfaI GTAC 6 cut(s) 24, 606, 1097, 1289, 1400, 1448
AfiI CCNNNNNNNGG 3 cut(s) 685, 1008, 1376
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 7 cut(s) 90, 271, 629, 807, 1031, 1086, 1318
AhlI ACTAGT 1 cut(s) 1112
AjiI CACGTC 1 cut(s) 877
AjnI CCWGG 3 cut(s) 280, 1089, 1250
AleI CACNNNNGTG 1 cut(s) 1023
AluBI AGCT 5 cut(s) 98, 208, 239, 501, 1313
AluI AGCT 5 cut(s) 98, 208, 239, 501, 1313
Alw21I GWGCWC 2 cut(s) 241, 1162
Alw26I GTCTC 1 cut(s) 362
AlwI GGATC 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 4 cut(s) 277, 1150, 1248, 1381
ApeKI GCWGC 3 cut(s) 98, 205, 1391
ApoI RAATTY 3 cut(s) 506, 550, 714
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
Asp718I GGTACC 1 cut(s) 22
AspS9I GGNCC 3 cut(s) 278, 320, 962
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 5 cut(s) 158, 200, 581, 1010, 1187
AvaII GGWCC 2 cut(s) 320, 962
BaeGI GKGCMC 1 cut(s) 1058
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 1118
Bbv12I GWGCWC 2 cut(s) 241, 1162
BbvI GCAGC 3 cut(s) 85, 217, 1378
BccI CCATC 6 cut(s) 34, 381, 614, 735, 1428, 1432
BciT130I CCWGG 3 cut(s) 282, 1091, 1252
BclI TGATCA 1 cut(s) 1177
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 362
BcuI ACTAGT 1 cut(s) 1112
BfaI CTAG 1 cut(s) 1113
BfmI CTRYAG 5 cut(s) 243, 512, 1168, 1281, 1392
BglI GCCNNNNNGGC 1 cut(s) 1256
BisI GCNGC 4 cut(s) 99, 206, 765, 1392
BlsI GCNGC 4 cut(s) 100, 207, 766, 1393
Bme1390I CCNGG 4 cut(s) 135, 282, 1091, 1252
Bme18I GGWCC 2 cut(s) 320, 962
BmgBI CACGTC 1 cut(s) 877
BmgT120I GGNCC 3 cut(s) 278, 320, 962
BmiI GGNNCC 3 cut(s) 24, 677, 885
BmrFI CCNGG 4 cut(s) 135, 282, 1091, 1252
BmrI ACTGGG 1 cut(s) 674
BmsI GCATC 5 cut(s) 409, 691, 826, 835, 1024
BmuI ACTGGG 1 cut(s) 674
BpiI GAAGAC 1 cut(s) 1118
BpmI CTGGAG 1 cut(s) 1273
BpuEI CTTGAG 3 cut(s) 641, 905, 1086
BpuMI CCSGG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 834
BsaAI YACGTR 2 cut(s) 793, 1157
BsaHI GRCGYC 2 cut(s) 399, 1044
BsaJI CCNNGG 2 cut(s) 280, 1089
BsaWI WCCGGW 2 cut(s) 143, 170
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 3 cut(s) 685, 1008, 1376
Bse1I ACTGG 5 cut(s) 168, 323, 680, 887, 964
Bse3DI GCAATG 4 cut(s) 817, 1200, 1386, 1428
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 3 cut(s) 282, 1091, 1252
BseCI ATCGAT 1 cut(s) 834
BseDI CCNNGG 2 cut(s) 280, 1089
BseGI GGATG 4 cut(s) 481, 625, 1150, 1443
BseLI CCNNNNNNNGG 3 cut(s) 685, 1008, 1376
BseMI GCAATG 4 cut(s) 817, 1200, 1386, 1428
BseMII CTCAG 2 cut(s) 222, 1457
BseNI ACTGG 5 cut(s) 168, 323, 680, 887, 964
BseRI GAGGAG 1 cut(s) 1392
BseSI GKGCMC 1 cut(s) 1058
BseXI GCAGC 3 cut(s) 85, 217, 1378
BseYI CCCAGC 1 cut(s) 361
BsgI GTGCAG 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 1142
BshFI GGCC 4 cut(s) 279, 1152, 1250, 1383
BshNI GGYRCC 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 834
BsiHKAI GWGCWC 2 cut(s) 241, 1162
BsiSI CCGG 3 cut(s) 134, 144, 171
BslI CCNNNNNNNGG 3 cut(s) 685, 1008, 1376
BsmAI GTCTC 1 cut(s) 362
BsmI GAATGC 2 cut(s) 712, 1418
BsnI GGCC 4 cut(s) 279, 1152, 1250, 1383
Bsp1286I GDGCHC 3 cut(s) 241, 1058, 1162
Bsp13I TCCGGA 1 cut(s) 170
Bsp1407I TGTACA 1 cut(s) 604
Bsp143I GATC 4 cut(s) 250, 748, 1164, 1177
BspACI CCGC 3 cut(s) 462, 764, 1142
BspANI GGCC 4 cut(s) 279, 1152, 1250, 1383
BspCNI CTCAG 2 cut(s) 223, 1456
BspDI ATCGAT 1 cut(s) 834
BspEI TCCGGA 1 cut(s) 170
BspFNI CGCG 1 cut(s) 1142
BspLI GGNNCC 3 cut(s) 24, 677, 885
BspMAI CTGCAG 4 cut(s) 247, 516, 1285, 1396
BspPI GGATC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 4 cut(s) 817, 1200, 1386, 1428
BsrGI TGTACA 1 cut(s) 604
BsrI ACTGG 5 cut(s) 168, 323, 680, 887, 964
BssECI CCNNGG 2 cut(s) 280, 1089
BssMI GATC 4 cut(s) 250, 748, 1164, 1177
BssNI GRCGYC 2 cut(s) 399, 1044
Bst2UI CCWGG 3 cut(s) 282, 1091, 1252
Bst4CI ACNGT 4 cut(s) 57, 756, 1079, 1172
BstACI GRCGYC 2 cut(s) 399, 1044
BstAPI GCANNNNNTGC 1 cut(s) 536
BstAUI TGTACA 1 cut(s) 604
BstBAI YACGTR 2 cut(s) 793, 1157
BstC8I GCNNGC 4 cut(s) 103, 820, 999, 1345
BstDEI CTNAG 3 cut(s) 231, 944, 1443
BstEII GGTNACC 1 cut(s) 146
BstENI CCTNNNNNAGG 1 cut(s) 1374
BstF5I GGATG 4 cut(s) 481, 625, 1150, 1443
BstFNI CGCG 1 cut(s) 1142
BstKTI GATC 4 cut(s) 253, 751, 1167, 1180
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 4 cut(s) 250, 748, 1164, 1177
BstMWI GCNNNNNNNGC 6 cut(s) 285, 520, 536, 845, 1256, 1310
BstNI CCWGG 3 cut(s) 282, 1091, 1252
BstNSI RCATGY 1 cut(s) 822
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 4 cut(s) 133, 280, 1089, 1250
BstSFI CTRYAG 5 cut(s) 243, 512, 1168, 1281, 1392
BstSLI GKGCMC 1 cut(s) 1058
BstSNI TACGTA 1 cut(s) 793
BstUI CGCG 1 cut(s) 1142
BstV1I GCAGC 3 cut(s) 85, 217, 1378
BstV2I GAAGAC 1 cut(s) 1118
BstXI CCANNNNNNTGG 1 cut(s) 387
Bsu15I ATCGAT 1 cut(s) 834
BsuRI GGCC 4 cut(s) 279, 1152, 1250, 1383
BsuTUI ATCGAT 1 cut(s) 834
BtrI CACGTC 1 cut(s) 877
BtsCI GGATG 4 cut(s) 481, 625, 1150, 1443
BtsI GCAGTG 2 cut(s) 787, 903
BtsIMutI CAGTG 5 cut(s) 161, 787, 894, 903, 1177
Cac8I GCNNGC 4 cut(s) 103, 820, 999, 1345
Cfr13I GGNCC 3 cut(s) 278, 320, 962
ClaI ATCGAT 1 cut(s) 834
CseI GACGC 3 cut(s) 536, 824, 1033
Csp6I GTAC 6 cut(s) 23, 605, 1096, 1288, 1399, 1447
CviAII CATG 4 cut(s) 406, 819, 1186, 1260
CviQI GTAC 6 cut(s) 23, 605, 1096, 1288, 1399, 1447
DdeI CTNAG 3 cut(s) 231, 944, 1443
DpnI GATC 4 cut(s) 252, 750, 1166, 1179
DpnII GATC 4 cut(s) 250, 748, 1164, 1177
Ecl136II GAGCTC 1 cut(s) 239
Eco105I TACGTA 1 cut(s) 793
Eco147I AGGCCT 1 cut(s) 1250
Eco24I GRGCYC 1 cut(s) 241
Eco32I GATATC 1 cut(s) 917
Eco47I GGWCC 2 cut(s) 320, 962
Eco53kI GAGCTC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 239
EcoNI CCTNNNNNAGG 1 cut(s) 1374
EcoO65I GGTNACC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 3 cut(s) 280, 1089, 1250
EcoRV GATATC 1 cut(s) 917
EcoT22I ATGCAT 1 cut(s) 824
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 4 cut(s) 409, 822, 1189, 1263
FalI AAGNNNNNCTT 6 cut(s) 703, 735, 1023, 1055, 1336, 1368
FatI CATG 4 cut(s) 405, 818, 1185, 1259
FauNDI CATATG 1 cut(s) 993
FbaI TGATCA 1 cut(s) 1177
Fnu4HI GCNGC 4 cut(s) 99, 206, 765, 1392
FokI GGATG 4 cut(s) 488, 632, 1157, 1450
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 4 cut(s) 99, 206, 765, 1392
FspBI CTAG 1 cut(s) 1113
GluI GCNGC 4 cut(s) 99, 206, 765, 1392
GsaI CCCAGC 1 cut(s) 365
GsuI CTGGAG 1 cut(s) 1273
HaeIII GGCC 4 cut(s) 279, 1152, 1250, 1383
HapII CCGG 3 cut(s) 134, 144, 171
HgaI GACGC 3 cut(s) 536, 824, 1033
Hin1I GRCGYC 2 cut(s) 399, 1044
Hin1II CATG 4 cut(s) 409, 822, 1189, 1263
HpaII CCGG 3 cut(s) 134, 144, 171
HphI GGTGA 5 cut(s) 158, 200, 581, 1010, 1187
Hpy166II GTNNAC 4 cut(s) 796, 941, 1098, 1290
Hpy188I TCNGA 8 cut(s) 232, 340, 748, 829, 934, 1049, 1164, 1242
Hpy188III TCNNGA 6 cut(s) 171, 371, 582, 1031, 1103, 1181
Hpy8I GTNNAC 4 cut(s) 796, 941, 1098, 1290
HpyAV CCTTC 5 cut(s) 161, 586, 893, 929, 1044
HpyCH4III ACNGT 4 cut(s) 57, 756, 1079, 1172
HpyCH4IV ACGT 7 cut(s) 399, 792, 798, 861, 876, 1156, 1325
HpyF10VI GCNNNNNNNGC 6 cut(s) 285, 520, 536, 845, 1256, 1310
HpyF3I CTNAG 3 cut(s) 231, 944, 1443
HpySE526I ACGT 7 cut(s) 399, 792, 798, 861, 876, 1156, 1325
Hsp92I GRCGYC 2 cut(s) 399, 1044
Hsp92II CATG 4 cut(s) 409, 822, 1189, 1263
Kpn2I TCCGGA 1 cut(s) 170
KpnI GGTACC 1 cut(s) 26
Ksp22I TGATCA 1 cut(s) 1177
Kzo9I GATC 4 cut(s) 250, 748, 1164, 1177
LmnI GCTCC 1 cut(s) 244
Lsp1109I GCAGC 3 cut(s) 85, 217, 1378
LweI GCATC 5 cut(s) 409, 691, 826, 835, 1024
MaeI CTAG 1 cut(s) 1113
MaeII ACGT 7 cut(s) 399, 792, 798, 861, 876, 1156, 1325
MaeIII GTNAC 6 cut(s) 146, 188, 325, 569, 682, 1108
MalI GATC 4 cut(s) 252, 750, 1166, 1179
MboI GATC 4 cut(s) 250, 748, 1164, 1177
MboII GAAGA 5 cut(s) 41, 209, 298, 924, 1118
MfeI CAATTG 1 cut(s) 1006
MhlI GDGCHC 3 cut(s) 241, 1058, 1162
MlyI GAGTC 4 cut(s) 236, 364, 566, 1231
Mph1103I ATGCAT 1 cut(s) 824
MroI TCCGGA 1 cut(s) 170
MseI TTAA 2 cut(s) 59, 333
MslI CAYNNNNRTG 4 cut(s) 385, 973, 1023, 1213
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 3 cut(s) 134, 144, 171
MspR9I CCNGG 4 cut(s) 135, 282, 1091, 1252
MunI CAATTG 1 cut(s) 1006
Mva1269I GAATGC 2 cut(s) 712, 1418
MvaI CCWGG 3 cut(s) 282, 1091, 1252
MvnI CGCG 1 cut(s) 1142
MwoI GCNNNNNNNGC 6 cut(s) 285, 520, 536, 845, 1256, 1310
NciI CCSGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 993
NdeII GATC 4 cut(s) 250, 748, 1164, 1177
NlaIII CATG 4 cut(s) 409, 822, 1189, 1263
NlaIV GGNNCC 3 cut(s) 24, 677, 885
NmuCI GTSAC 4 cut(s) 146, 188, 569, 1108
NsiI ATGCAT 1 cut(s) 824
NspI RCATGY 1 cut(s) 822
OliI CACNNNNGTG 1 cut(s) 1023
PaeI GCATGC 1 cut(s) 822
PceI AGGCCT 1 cut(s) 1250
PctI GAATGC 2 cut(s) 712, 1418
PfeI GAWTC 7 cut(s) 127, 167, 303, 438, 652, 689, 803
PkrI GCNGC 4 cut(s) 100, 207, 766, 1393
PleI GAGTC 4 cut(s) 235, 363, 566, 1231
PpsI GAGTC 4 cut(s) 235, 363, 566, 1231
Ppu21I YACGTR 2 cut(s) 793, 1157
PsiI TTATAA 1 cut(s) 987
Psp124BI GAGCTC 1 cut(s) 241
Psp6I CCWGG 3 cut(s) 280, 1089, 1250
PspEI GGTNACC 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 361
PspGI CCWGG 3 cut(s) 280, 1089, 1250
PspN4I GGNNCC 3 cut(s) 24, 677, 885
PspPI GGNCC 3 cut(s) 278, 320, 962
PstI CTGCAG 4 cut(s) 247, 516, 1285, 1396
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 6 cut(s) 24, 606, 1097, 1289, 1400, 1448
RsaNI GTAC 6 cut(s) 23, 605, 1096, 1288, 1399, 1447
RseI CAYNNNNRTG 4 cut(s) 385, 973, 1023, 1213
SacI GAGCTC 1 cut(s) 241
SaqAI TTAA 2 cut(s) 59, 333
SatI GCNGC 4 cut(s) 99, 206, 765, 1392
Sau3AI GATC 4 cut(s) 250, 748, 1164, 1177
Sau96I GGNCC 3 cut(s) 278, 320, 962
SchI GAGTC 4 cut(s) 236, 364, 566, 1231
ScrFI CCNGG 4 cut(s) 135, 282, 1091, 1252
SduI GDGCHC 3 cut(s) 241, 1058, 1162
SfaNI GCATC 5 cut(s) 409, 691, 826, 835, 1024
SfcI CTRYAG 5 cut(s) 243, 512, 1168, 1281, 1392
SinI GGWCC 2 cut(s) 320, 962
SmiMI CAYNNNNRTG 4 cut(s) 385, 973, 1023, 1213
SmlI CTYRAG 3 cut(s) 656, 920, 1101
SmoI CTYRAG 3 cut(s) 656, 920, 1101
SnaBI TACGTA 1 cut(s) 793
SpeI ACTAGT 1 cut(s) 1112
SphI GCATGC 1 cut(s) 822
SseBI AGGCCT 1 cut(s) 1250
SsiI CCGC 3 cut(s) 462, 764, 1142
SspI AATATT 1 cut(s) 615
SspMI CTAG 1 cut(s) 1113
SstI GAGCTC 1 cut(s) 241
StuI AGGCCT 1 cut(s) 1250
StyD4I CCNGG 4 cut(s) 133, 280, 1089, 1250
TaaI ACNGT 4 cut(s) 57, 756, 1079, 1172
TaiI ACGT 7 cut(s) 402, 795, 801, 864, 879, 1159, 1328
TaqI TCGA 3 cut(s) 77, 385, 834
TatI WGTACW 2 cut(s) 604, 1398
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 7 cut(s) 127, 167, 303, 438, 652, 689, 803
Tru1I TTAA 2 cut(s) 59, 333
Tru9I TTAA 2 cut(s) 59, 333
TscAI CASTG 5 cut(s) 168, 787, 894, 910, 1177
TseFI GTSAC 4 cut(s) 146, 188, 569, 1108
TseI GCWGC 3 cut(s) 98, 205, 1391
Tsp45I GTSAC 4 cut(s) 146, 188, 569, 1108
TspDTI ATGAA 7 cut(s) 47, 210, 519, 665, 681, 685, 1118
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 5 cut(s) 168, 787, 894, 910, 1177
VpaK11BI GGWCC 2 cut(s) 320, 962
XagI CCTNNNNNAGG 1 cut(s) 1374
XapI RAATTY 3 cut(s) 506, 550, 714
XceI RCATGY 1 cut(s) 822
XspI CTAG 1 cut(s) 1113
ZraI GACGTC 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.