Rroxscaffold_5G00343300

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000839
Physical Location & Seq
Forward (+)
12626794 .. 12630340
3547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343300.1

Sequence Viewer

Length: 1434 bp
ATGTTGCCCAAAATGAATATGTCCATGGGTTTGCGTCTACTTGTGACATTATTGGGTTTAATCACTTCGACTTCAGCTTCAAACATCACTACGAAAATCGTTACCACTCTACCCGGTTACTCTGGAGATCTTCCTTTCACATTAGAAACCGGATATATCGGCGTAGGTGACAATGAAGAAGTGCAGCTATTTTACGTTTTTGTCGAGTCTGAGAGAAACCCTGCACAAGATCCACTTGTGTTACGATTTGGTGCCGGTCCGGGTTGCTCTGGTCTCCACGGCTTTTTCTTTGAGAGTGGTCCATTATCTTTCAATTCTTCACATTACAACGGGAGCCTACCAACTCTTGTGGACAACCCTTATTCATGGACCAAGGGACTCAACATCATATTCTTGGATGCACCAGCTGGTACTGGATTCTCCTATTCAACAACCCAAGATGGTTACTATGTGGATGAATATAAATATGCGGCACAAGGCTATGAATTCCTCCAGAAGTGGCTGGAGGCACACCCTGCATATGTGGAAAATCTACTCTACATTAGTGGTGAATCCTTCAGCGGCTTAATAGTTCCAATGGTTGTACAAAAGATAGTAGATGGTAATGAAAATGGAAGTGTGCCTCTAATGAATATTAAGGGATATCTGCTTCAGAACCCAGTAACGGATTCATTTATTGATGGTAATGCAGTAATCCCTTATGCCCACCGGCTGACTCTTGTATCTGATCAACTTTATAAGGCGGTTAAAACAAGTTGCAATGGTGAATACGTGAATGTAAATTCAAGCAATGCTGCATGCATCGCTGATATCGATGCTATTGATCAGCTCATCAAGGATATAAATAAGAACAATATCTTGGAACCAGACTGTACTACTTCATTGCCGAGATCAAAGAAGATAAGCACAGCTCGAAGATATCTCAAAGAGTTGTCCAAAGGTTTACTGAGTTCACCAACCAACGGTCCCGCACTTTGGTGTCGGGAGTATAATTACATGCTCCTATACATCTGGGCAAACACTAAAGCTGTCCGAGATGCTCTTGGTGTCGGAATGGGCACAAAATTGTTATGGAGACCATGTAATACCACATTGACGGGTTACACTCATGAAGTCACCAGTTCTCTCAGTTATCATCTCAATCTCACAAAAAAGGCTGACCTACGTGCTTTGATCTACAGCGGTGACCATGACATGTCTGTTCCTCATATTGGTACACAAGCATGGATAAATATACTGAATTTGACTGTGGATGAGTCCTGGAGGACATGGTCCCTTGATGGACAAACTAAAGGATACACGAAGAAGTTAATAAATGATTTTTTCACGGTGGTATTTGCGACTGTGAAGGCAACAGGCCACCTTGCCGCAGAGTACCAGCCAAAGGAAGTTGCTAGCTTGATTGATAGATGGATGGCTTACTACCCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

477

Amino Acids

52.86

Weight (kDa)

5.75

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 472 1e-109 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 37
AciI CCGC 6 cut(s) 470, 561, 743, 969, 1182, 1368
AclWI GGATC 1 cut(s) 224
AcsI RAATTY 3 cut(s) 485, 781, 1240
AcuI CTGAAG 3 cut(s) 57, 541, 635
AfaI GTAC 5 cut(s) 412, 585, 874, 1216, 1376
AfiI CCNNNNNNNGG 5 cut(s) 664, 962, 975, 1211, 1384
AflIII ACRYGT 1 cut(s) 1194
AgsI TTSAA 4 cut(s) 81, 313, 429, 786
AjnI CCWGG 1 cut(s) 1259
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AleI CACNNNNGTG 1 cut(s) 976
AluBI AGCT 7 cut(s) 77, 187, 407, 829, 911, 1028, 1398
AluI AGCT 7 cut(s) 77, 187, 407, 829, 911, 1028, 1398
Alw26I GTCTC 2 cut(s) 278, 1069
AlwI GGATC 1 cut(s) 224
AoxI GGCC 1 cut(s) 1357
ApeKI GCWGC 2 cut(s) 184, 794
ApoI RAATTY 3 cut(s) 485, 781, 1240
AspS9I GGNCC 5 cut(s) 257, 299, 369, 965, 1272
AsuC2I CCSGG 2 cut(s) 114, 261
AsuHPI GGTGA 6 cut(s) 179, 560, 776, 945, 1108, 1196
AsuNHI GCTAGC 1 cut(s) 1394
AvaII GGWCC 5 cut(s) 257, 299, 369, 965, 1272
BaeGI GKGCMC 1 cut(s) 1061
BaeI ACNNNNGTAYC 2 cut(s) 705, 738
BanI GGYRCC 1 cut(s) 251
BbvI GCAGC 2 cut(s) 196, 781
BccI CCATC 6 cut(s) 434, 593, 674, 1274, 1404, 1408
BceAI ACGGC 1 cut(s) 295
BciT130I CCWGG 1 cut(s) 1261
BciVI GTATCC 1 cut(s) 1289
BclI TGATCA 2 cut(s) 727, 823
BcnI CCSGG 2 cut(s) 114, 261
BcoDI GTCTC 2 cut(s) 278, 1069
BfaI CTAG 1 cut(s) 1395
BfmI CTRYAG 1 cut(s) 1177
BfuI GTATCC 1 cut(s) 1289
BglII AGATCT 1 cut(s) 127
BisI GCNGC 5 cut(s) 185, 471, 562, 795, 1368
BlsI GCNGC 5 cut(s) 186, 472, 563, 796, 1369
Bme1390I CCNGG 3 cut(s) 114, 261, 1261
Bme18I GGWCC 5 cut(s) 257, 299, 369, 965, 1272
BmgT120I GGNCC 5 cut(s) 257, 299, 369, 965, 1272
BmiI GGNNCC 5 cut(s) 253, 335, 864, 967, 1274
BmrFI CCNGG 3 cut(s) 114, 261, 1261
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 4 cut(s) 388, 805, 810, 1027
BmtI GCTAGC 1 cut(s) 1398
BmuI ACTGGG 1 cut(s) 653
BpmI CTGGAG 4 cut(s) 144, 476, 524, 1282
BpuMI CCSGG 2 cut(s) 114, 261
Bsa29I ATCGAT 1 cut(s) 813
BsaAI YACGTR 2 cut(s) 772, 1166
BsaI GGTCTC 2 cut(s) 278, 1069
BsaJI CCNNGG 3 cut(s) 24, 277, 372
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 1066, 1096
Bsc4I CCNNNNNNNGG 5 cut(s) 664, 962, 975, 1211, 1384
Bse118I RCCGGY 2 cut(s) 254, 708
Bse1I ACTGG 3 cut(s) 418, 659, 1119
Bse3DI GCAATG 3 cut(s) 766, 796, 881
BseBI CCWGG 1 cut(s) 1261
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 3 cut(s) 24, 277, 372
BseGI GGATG 4 cut(s) 403, 460, 1258, 1419
BseLI CCNNNNNNNGG 5 cut(s) 664, 962, 975, 1211, 1384
BseMI GCAATG 3 cut(s) 766, 796, 881
BseMII CTCAG 3 cut(s) 201, 938, 1141
BseNI ACTGG 3 cut(s) 418, 659, 1119
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 2 cut(s) 196, 781
BsgI GTGCAG 2 cut(s) 203, 207
BshFI GGCC 1 cut(s) 1359
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 813
BsiSI CCGG 5 cut(s) 114, 150, 255, 260, 709
BslFI GGGAC 3 cut(s) 390, 951, 1258
BslI CCNNNNNNNGG 5 cut(s) 664, 962, 975, 1211, 1384
BsmAI GTCTC 2 cut(s) 278, 1069
BsmFI GGGAC 3 cut(s) 390, 951, 1258
BsnI GGCC 1 cut(s) 1359
Bso31I GGTCTC 2 cut(s) 278, 1069
Bsp1286I GDGCHC 1 cut(s) 1061
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 6 cut(s) 127, 229, 727, 823, 890, 1173
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 6 cut(s) 470, 561, 743, 969, 1182, 1368
BspANI GGCC 1 cut(s) 1359
BspCNI CTCAG 3 cut(s) 202, 939, 1140
BspDI ATCGAT 1 cut(s) 813
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 5 cut(s) 253, 335, 864, 967, 1274
BspOI GCTAGC 1 cut(s) 1398
BspPI GGATC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 251
BspTNI GGTCTC 2 cut(s) 278, 1069
BsrDI GCAATG 3 cut(s) 766, 796, 881
BsrFI RCCGGY 2 cut(s) 254, 708
BsrGI TGTACA 1 cut(s) 583
BsrI ACTGG 3 cut(s) 418, 659, 1119
BssAI RCCGGY 2 cut(s) 254, 708
BssECI CCNNGG 3 cut(s) 24, 277, 372
BssMI GATC 6 cut(s) 127, 229, 727, 823, 890, 1173
BssT1I CCWWGG 2 cut(s) 24, 372
Bst2UI CCWGG 1 cut(s) 1261
Bst4CI ACNGT 5 cut(s) 872, 965, 1249, 1330, 1345
BstAPI GCANNNNNTGC 1 cut(s) 515
BstAUI TGTACA 1 cut(s) 583
BstBAI YACGTR 2 cut(s) 772, 1166
BstC8I GCNNGC 2 cut(s) 799, 1396
BstDEI CTNAG 3 cut(s) 210, 947, 1127
BstDSI CCRYGG 2 cut(s) 24, 277
BstEII GGTNACC 1 cut(s) 1184
BstF5I GGATG 4 cut(s) 403, 460, 1258, 1419
BstKTI GATC 6 cut(s) 130, 232, 730, 826, 893, 1176
BstMAI GTCTC 2 cut(s) 278, 1069
BstMBI GATC 6 cut(s) 127, 229, 727, 823, 890, 1173
BstMWI GCNNNNNNNGC 2 cut(s) 515, 803
BstNI CCWGG 1 cut(s) 1261
BstNSI RCATGY 3 cut(s) 801, 1000, 1198
BstPI GGTNACC 1 cut(s) 1184
BstSCI CCNGG 3 cut(s) 112, 259, 1259
BstSFI CTRYAG 1 cut(s) 1177
BstSLI GKGCMC 1 cut(s) 1061
BstV1I GCAGC 2 cut(s) 196, 781
BstX2I RGATCY 2 cut(s) 127, 229
BstYI RGATCY 2 cut(s) 127, 229
Bsu15I ATCGAT 1 cut(s) 813
BsuI GTATCC 1 cut(s) 1289
BsuRI GGCC 1 cut(s) 1359
BsuTUI ATCGAT 1 cut(s) 813
BtgI CCRYGG 2 cut(s) 24, 277
BtgZI GCGATG 1 cut(s) 787
BtsCI GGATG 4 cut(s) 403, 460, 1258, 1419
Cac8I GCNNGC 2 cut(s) 799, 1396
CciI TCATGA 1 cut(s) 1108
Cfr10I RCCGGY 2 cut(s) 254, 708
Cfr13I GGNCC 5 cut(s) 257, 299, 369, 965, 1272
ClaI ATCGAT 1 cut(s) 813
CpoI CGGWCCG 1 cut(s) 257
CseI GACGC 1 cut(s) 23
Csp6I GTAC 5 cut(s) 411, 584, 873, 1215, 1375
CspCI CAANNNNNGTGG 2 cut(s) 330, 365
CspI CGGWCCG 1 cut(s) 257
CviQI GTAC 5 cut(s) 411, 584, 873, 1215, 1375
DdeI CTNAG 3 cut(s) 210, 947, 1127
DpnI GATC 6 cut(s) 129, 231, 729, 825, 892, 1175
DpnII GATC 6 cut(s) 127, 229, 727, 823, 890, 1173
Eco130I CCWWGG 2 cut(s) 24, 372
Eco31I GGTCTC 2 cut(s) 278, 1069
Eco32I GATATC 3 cut(s) 644, 811, 920
Eco47I GGWCC 5 cut(s) 257, 299, 369, 965, 1272
Eco57I CTGAAG 3 cut(s) 57, 541, 635
Eco91I GGTNACC 1 cut(s) 1184
EcoO65I GGTNACC 1 cut(s) 1184
EcoRI GAATTC 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 1259
EcoRV GATATC 3 cut(s) 644, 811, 920
EcoT14I CCWWGG 2 cut(s) 24, 372
EcoT22I ATGCAT 1 cut(s) 803
ErhI CCWWGG 2 cut(s) 24, 372
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FaqI GGGAC 3 cut(s) 390, 951, 1258
FauI CCCGC 1 cut(s) 976
FauNDI CATATG 1 cut(s) 520
FbaI TGATCA 2 cut(s) 727, 823
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 5 cut(s) 185, 471, 562, 795, 1368
FokI GGATG 4 cut(s) 410, 467, 1265, 1426
Fsp4HI GCNGC 5 cut(s) 185, 471, 562, 795, 1368
FspBI CTAG 1 cut(s) 1395
GluI GCNGC 5 cut(s) 185, 471, 562, 795, 1368
GsuI CTGGAG 4 cut(s) 144, 476, 524, 1282
HaeIII GGCC 1 cut(s) 1359
HapII CCGG 5 cut(s) 114, 150, 255, 260, 709
HgaI GACGC 1 cut(s) 23
HinfI GANTC 7 cut(s) 206, 378, 417, 551, 668, 715, 1256
HpaII CCGG 5 cut(s) 114, 150, 255, 260, 709
HphI GGTGA 6 cut(s) 179, 560, 776, 945, 1108, 1196
Hpy166II GTNNAC 5 cut(s) 38, 352, 944, 953, 1217
Hpy188I TCNGA 5 cut(s) 211, 654, 727, 1034, 1052
Hpy188III TCNNGA 4 cut(s) 123, 493, 983, 1109
Hpy8I GTNNAC 5 cut(s) 38, 352, 944, 953, 1217
HpyAV CCTTC 2 cut(s) 565, 1342
HpyCH4III ACNGT 5 cut(s) 872, 965, 1249, 1330, 1345
HpyCH4IV ACGT 3 cut(s) 195, 771, 1165
HpyCH4V TGCA 8 cut(s) 184, 224, 401, 518, 689, 759, 797, 801
HpyF10VI GCNNNNNNNGC 2 cut(s) 515, 803
HpyF3I CTNAG 3 cut(s) 210, 947, 1127
HpySE526I ACGT 3 cut(s) 195, 771, 1165
Ksp22I TGATCA 2 cut(s) 727, 823
Kzo9I GATC 6 cut(s) 127, 229, 727, 823, 890, 1173
LmnI GCTCC 2 cut(s) 333, 1005
Lsp1109I GCAGC 2 cut(s) 196, 781
LweI GCATC 4 cut(s) 388, 805, 810, 1027
MaeI CTAG 1 cut(s) 1395
MaeII ACGT 3 cut(s) 195, 771, 1165
MalI GATC 6 cut(s) 129, 231, 729, 825, 892, 1175
MboI GATC 6 cut(s) 127, 229, 727, 823, 890, 1173
MboII GAAGA 6 cut(s) 122, 188, 309, 910, 927, 1315
MflI RGATCY 2 cut(s) 127, 229
MhlI GDGCHC 1 cut(s) 1061
MluCI AATT 6 cut(s) 313, 485, 781, 991, 1064, 1240
MlyI GAGTC 4 cut(s) 215, 372, 709, 1265
MmeI TCCRAC 1 cut(s) 1030
MnlI CCTC 5 cut(s) 499, 500, 633, 1215, 1257
Mph1103I ATGCAT 1 cut(s) 803
MseI TTAA 6 cut(s) 59, 566, 636, 747, 1310, 1432
MslI CAYNNNNRTG 2 cut(s) 976, 1222
MspA1I CMGCKG 3 cut(s) 407, 561, 1182
MspI CCGG 5 cut(s) 114, 150, 255, 260, 709
MspR9I CCNGG 3 cut(s) 114, 261, 1261
MvaI CCWGG 1 cut(s) 1261
MwoI GCNNNNNNNGC 2 cut(s) 515, 803
NciI CCSGG 2 cut(s) 114, 261
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 520
NdeII GATC 6 cut(s) 127, 229, 727, 823, 890, 1173
NheI GCTAGC 1 cut(s) 1394
NlaIV GGNNCC 5 cut(s) 253, 335, 864, 967, 1274
NmeAIII GCCGAG 1 cut(s) 912
NmuCI GTSAC 4 cut(s) 43, 167, 1114, 1184
NsiI ATGCAT 1 cut(s) 803
NspI RCATGY 3 cut(s) 801, 1000, 1198
OliI CACNNNNGTG 1 cut(s) 976
PaeI GCATGC 1 cut(s) 801
PagI TCATGA 1 cut(s) 1108
PciI ACATGT 1 cut(s) 1194
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 810
PfeI GAWTC 3 cut(s) 417, 551, 668
PflFI GACNNNGTC 1 cut(s) 1270
PfoI TCCNGGA 1 cut(s) 1259
PkrI GCNGC 5 cut(s) 186, 472, 563, 796, 1369
PleI GAGTC 4 cut(s) 214, 372, 709, 1264
PpsI GAGTC 4 cut(s) 214, 372, 709, 1264
Ppu21I YACGTR 2 cut(s) 772, 1166
PscI ACATGT 1 cut(s) 1194
PsiI TTATAA 1 cut(s) 738
Psp6I CCWGG 1 cut(s) 1259
PspEI GGTNACC 1 cut(s) 1184
PspGI CCWGG 1 cut(s) 1259
PspN4I GGNNCC 5 cut(s) 253, 335, 864, 967, 1274
PspPI GGNCC 5 cut(s) 257, 299, 369, 965, 1272
PsuI RGATCY 2 cut(s) 127, 229
PsyI GACNNNGTC 1 cut(s) 1270
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 5 cut(s) 412, 585, 874, 1216, 1376
RsaNI GTAC 5 cut(s) 411, 584, 873, 1215, 1375
RseI CAYNNNNRTG 2 cut(s) 976, 1222
Rsr2I CGGWCCG 1 cut(s) 257
RsrII CGGWCCG 1 cut(s) 257
SaqAI TTAA 6 cut(s) 59, 566, 636, 747, 1310, 1432
SatI GCNGC 5 cut(s) 185, 471, 562, 795, 1368
Sau3AI GATC 6 cut(s) 127, 229, 727, 823, 890, 1173
Sau96I GGNCC 5 cut(s) 257, 299, 369, 965, 1272
SchI GAGTC 4 cut(s) 215, 372, 709, 1265
ScrFI CCNGG 3 cut(s) 114, 261, 1261
SduI GDGCHC 1 cut(s) 1061
SfaNI GCATC 4 cut(s) 388, 805, 810, 1027
SfcI CTRYAG 1 cut(s) 1177
SinI GGWCC 5 cut(s) 257, 299, 369, 965, 1272
SmiMI CAYNNNNRTG 2 cut(s) 976, 1222
SphI GCATGC 1 cut(s) 801
Sse9I AATT 6 cut(s) 313, 485, 781, 991, 1064, 1240
SsiI CCGC 6 cut(s) 470, 561, 743, 969, 1182, 1368
SspI AATATT 1 cut(s) 634
SspMI CTAG 1 cut(s) 1395
StyD4I CCNGG 3 cut(s) 112, 259, 1259
StyI CCWWGG 2 cut(s) 24, 372
TaaI ACNGT 5 cut(s) 872, 965, 1249, 1330, 1345
TaiI ACGT 3 cut(s) 198, 774, 1168
TaqI TCGA 4 cut(s) 68, 204, 813, 913
TasI AATT 6 cut(s) 313, 485, 781, 991, 1064, 1240
TatI WGTACW 2 cut(s) 583, 872
TauI GCSGC 3 cut(s) 473, 564, 1370
TfiI GAWTC 3 cut(s) 417, 551, 668
Tru1I TTAA 6 cut(s) 59, 566, 636, 747, 1310, 1432
Tru9I TTAA 6 cut(s) 59, 566, 636, 747, 1310, 1432
TseFI GTSAC 4 cut(s) 43, 167, 1114, 1184
TseI GCWGC 2 cut(s) 184, 794
Tsp45I GTSAC 4 cut(s) 43, 167, 1114, 1184
TspGWI ACGGA 1 cut(s) 680
Tth111I GACNNNGTC 1 cut(s) 1270
VpaK11BI GGWCC 5 cut(s) 257, 299, 369, 965, 1272
XapI RAATTY 3 cut(s) 485, 781, 1240
XceI RCATGY 3 cut(s) 801, 1000, 1198
XmiI GTMKAC 1 cut(s) 37
XspI CTAG 1 cut(s) 1395
Zsp2I ATGCAT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.