Rroxscaffold_5G00343320

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000839
Physical Location & Seq
Reverse (-)
12700401 .. 12707464
7064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343320.1

Sequence Viewer

Length: 1347 bp
ATGGGATTAACCGATCCCATGATCGGTGATATGACTTTGGGGAGGGTACCAGAGGGAGGTACCTCGACTCCTTTCTACCTCAATGAACCGAGGCTTTTACCCCCTCGTTTCTACCTCAATGATAGAGATGAATTTTCTGGTTTGGGGAGATTGTATCCGAGCACCGGGCCGGTGAAGAAGCAGAAGAACAATACCCGCTCCGGTCTGACATATGGCCTGGACCCTACCGATTGGTGGCTGTCGTTCTGCCACGTCACCAGGGGACAAAGAGACGATGAAGAAGTGCAGCTGTTTTACTATTTTATCGAGTCTCAGAGGAACCCTGCACAAGATCCTCTGCTGTATTGGATCACTGGAGGCCCTGGTTGCTCTAGTCTTGCTGGATTTTTCTTTGAGAGTGGTCCATTGGTTTTCAACTACAAAGATTACAATGGAAGCCTACCAACACTTCAGGACAATCCTTATGCATGGACACAGGGACTCAACATCATATACTTGGATGCACCAGTTGGTGCCGGATACTCCTACTCAGAAACCCAAAGTGGTTATGTTATGGATGACTATAAATTTGTGGCTCAAATTTATGAATTTCTGCAAAAGTGGCTAGAGGCACACCCGACATATTTGGAAAATCTACTCTATATTGGTGGTGACTCTTATTCTGGCATACCTATGCCCATGCTTGCAGAACAAATAATTGATGGTAATGAAAATGGAAGTGTGCCTATGATGAATCTCAAAGGATATGTGCTTGGGAACCCAGTAACTGATTCTGTCATTGATATGAATGCAAGAGTTACTTATGCTCACAGGTTGTCTCTTGTGTCAGATCAACTGTATAAGGCTGCTAAAACAAGTTGCAATGGAGATTATGTAAATGTAGATTCAAGCAATGATGCATGCATAGCAGATATTGATGCCATTTCTGAGAGTTATAATCACATGCTCGCCTCAGTTTGGGCAAACAATAAAGGTGTCCAAGATGCTCTTGGTGTCCGAAGGGGCACCAAACAGTTTTGGCAGTACTGTAATACCACATTGGCTTACACTAAAGTTGCCACAAGTGTAGTGAGTTATCATCAAATTCTCTCAAAGAAAGCTGACCTTCGTGCCCTGATATACAGTGGTGACCATGACATGTCAATTCCTCATATTGGTACACAAGAATGGATAAAGACTCTGAATTTGACTGTGGACGAGTCCTGGAGGACATGGTCAATTGATGGCCAAACTGCAGGATACACAAAGAAGTTGATCAATGAGGGTGCAGGCCACTTTGCGGCAGAGTATAAAGTCAAGGAATGTGCTGCCATGATTGATCGATGGATGGGTTACTTCCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

448

Amino Acids

50.33

Weight (kDa)

4.97

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 91 - 310 9.9e-74 Serine carboxypeptidase
Peptidase_S10 PF00450 311 - 443 3e-24 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 936
Acc65I GGTACC 2 cut(s) 46, 59
AccB1I GGYRCC 4 cut(s) 46, 59, 512, 1004
AccBSI CCGCTC 1 cut(s) 198
AciI CCGC 2 cut(s) 196, 1280
AclWI GGATC 3 cut(s) 8, 326, 356
AcoI YGGCCR 1 cut(s) 1225
AcsI RAATTY 6 cut(s) 131, 566, 579, 587, 1083, 1183
AcuI CTGAAG 1 cut(s) 434
AfaI GTAC 4 cut(s) 48, 61, 1025, 1159
AfiI CCNNNNNNNGG 7 cut(s) 23, 56, 164, 234, 957, 1154, 1279
AflIII ACRYGT 1 cut(s) 1137
AgsI TTSAA 2 cut(s) 415, 888
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 4 cut(s) 216, 257, 361, 1202
AluBI AGCT 2 cut(s) 289, 1100
AluI AGCT 2 cut(s) 289, 1100
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 3 cut(s) 264, 315, 822
AlwI GGATC 3 cut(s) 8, 326, 356
AlwNI CAGNNNCTG 1 cut(s) 767
AoxI GGCC 5 cut(s) 167, 214, 358, 1225, 1270
ApeKI GCWGC 3 cut(s) 286, 845, 1307
ApoI RAATTY 6 cut(s) 131, 566, 579, 587, 1083, 1183
ArsI GACNNNNNNTTYG 4 cut(s) 551, 583, 1168, 1200
Asp718I GGTACC 2 cut(s) 46, 59
AspS9I GGNCC 4 cut(s) 167, 220, 359, 401
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 5 cut(s) 38, 184, 247, 662, 1139
AvaII GGWCC 2 cut(s) 220, 401
BaeGI GKGCMC 2 cut(s) 1007, 1114
BalI TGGCCA 1 cut(s) 1227
BanI GGYRCC 4 cut(s) 46, 59, 512, 1004
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 3 cut(s) 298, 832, 1294
BccI CCATC 4 cut(s) 695, 1217, 1317, 1321
BciT130I CCWGG 4 cut(s) 218, 259, 363, 1204
BciVI GTATCC 3 cut(s) 165, 512, 1232
BclI TGATCA 1 cut(s) 1254
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 3 cut(s) 264, 315, 822
BfaI CTAG 2 cut(s) 372, 605
BfmI CTRYAG 1 cut(s) 1233
BfuI GTATCC 3 cut(s) 165, 512, 1232
BisI GCNGC 4 cut(s) 287, 846, 1281, 1308
BlsI GCNGC 4 cut(s) 288, 847, 1282, 1309
BmcAI AGTACT 1 cut(s) 1025
Bme1390I CCNGG 5 cut(s) 166, 218, 259, 363, 1204
Bme18I GGWCC 2 cut(s) 220, 401
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 4 cut(s) 167, 220, 359, 401
BmiI GGNNCC 7 cut(s) 48, 61, 222, 320, 514, 758, 1006
BmrFI CCNGG 5 cut(s) 166, 218, 259, 363, 1204
BmrI ACTGGG 1 cut(s) 755
BmsI GCATC 4 cut(s) 490, 886, 907, 973
BmuI ACTGGG 1 cut(s) 755
BpmI CTGGAG 2 cut(s) 375, 1225
BpuMI CCSGG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 1321
BsaJI CCNNGG 3 cut(s) 89, 258, 361
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 4 cut(s) 52, 82, 858, 888
Bsc4I CCNNNNNNNGG 7 cut(s) 23, 56, 164, 234, 957, 1154, 1279
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 3 cut(s) 358, 506, 761
Bse3DI GCAATG 2 cut(s) 868, 898
BseBI CCWGG 4 cut(s) 218, 259, 363, 1204
BseCI ATCGAT 1 cut(s) 1321
BseDI CCNNGG 3 cut(s) 89, 258, 361
BseGI GGATG 3 cut(s) 505, 562, 1332
BseLI CCNNNNNNNGG 7 cut(s) 23, 56, 164, 234, 957, 1154, 1279
BseMI GCAATG 2 cut(s) 868, 898
BseMII CTCAG 4 cut(s) 326, 543, 918, 966
BseNI ACTGG 3 cut(s) 358, 506, 761
BseSI GKGCMC 2 cut(s) 1007, 1114
BseXI GCAGC 3 cut(s) 298, 832, 1294
BsgI GTGCAG 3 cut(s) 305, 309, 1287
BshFI GGCC 5 cut(s) 169, 216, 360, 1227, 1272
BshNI GGYRCC 4 cut(s) 46, 59, 512, 1004
BshVI ATCGAT 1 cut(s) 1321
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 4 cut(s) 165, 170, 201, 516
BslFI GGGAC 2 cut(s) 276, 492
BslI CCNNNNNNNGG 7 cut(s) 23, 56, 164, 234, 957, 1154, 1279
BsmAI GTCTC 3 cut(s) 264, 315, 822
BsmBI CGTCTC 1 cut(s) 264
BsmFI GGGAC 2 cut(s) 276, 492
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 5 cut(s) 169, 216, 360, 1227, 1272
Bsp1286I GDGCHC 3 cut(s) 164, 1007, 1114
Bsp143I GATC 7 cut(s) 13, 21, 331, 348, 829, 1254, 1318
BspACI CCGC 2 cut(s) 196, 1280
BspANI GGCC 5 cut(s) 169, 216, 360, 1227, 1272
BspCNI CTCAG 4 cut(s) 325, 542, 919, 965
BspDI ATCGAT 1 cut(s) 1321
BspLI GGNNCC 7 cut(s) 48, 61, 222, 320, 514, 758, 1006
BspMAI CTGCAG 1 cut(s) 1237
BspPI GGATC 3 cut(s) 8, 326, 356
BspT107I GGYRCC 4 cut(s) 46, 59, 512, 1004
BsrBI CCGCTC 1 cut(s) 198
BsrDI GCAATG 2 cut(s) 868, 898
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 3 cut(s) 358, 506, 761
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 89, 258, 361
BssMI GATC 7 cut(s) 13, 21, 331, 348, 829, 1254, 1318
Bst2UI CCWGG 4 cut(s) 218, 259, 363, 1204
Bst4CI ACNGT 5 cut(s) 837, 1014, 1028, 1124, 1192
BstC8I GCNNGC 4 cut(s) 684, 901, 948, 1270
BstDEI CTNAG 4 cut(s) 312, 529, 927, 952
BstEII GGTNACC 1 cut(s) 1127
BstF5I GGATG 3 cut(s) 505, 562, 1332
BstKTI GATC 7 cut(s) 16, 24, 334, 351, 832, 1257, 1321
BstMAI GTCTC 3 cut(s) 264, 315, 822
BstMBI GATC 7 cut(s) 13, 21, 331, 348, 829, 1254, 1318
BstMWI GCNNNNNNNGC 3 cut(s) 366, 601, 905
BstNI CCWGG 4 cut(s) 218, 259, 363, 1204
BstNSI RCATGY 3 cut(s) 903, 946, 1141
BstPI GGTNACC 1 cut(s) 1127
BstSCI CCNGG 5 cut(s) 164, 216, 257, 361, 1202
BstSFI CTRYAG 1 cut(s) 1233
BstSLI GKGCMC 2 cut(s) 1007, 1114
BstV1I GCAGC 3 cut(s) 298, 832, 1294
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
Bsu15I ATCGAT 1 cut(s) 1321
BsuI GTATCC 3 cut(s) 165, 512, 1232
BsuRI GGCC 5 cut(s) 169, 216, 360, 1227, 1272
BsuTUI ATCGAT 1 cut(s) 1321
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 3 cut(s) 505, 562, 1332
BtsIMutI CAGTG 2 cut(s) 351, 1129
Cac8I GCNNGC 4 cut(s) 684, 901, 948, 1270
CaiI CAGNNNCTG 1 cut(s) 767
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 4 cut(s) 167, 220, 359, 401
ClaI ATCGAT 1 cut(s) 1321
Csp6I GTAC 4 cut(s) 47, 60, 1024, 1158
CviAII CATG 9 cut(s) 19, 468, 679, 900, 943, 1133, 1138, 1212, 1312
CviQI GTAC 4 cut(s) 47, 60, 1024, 1158
DdeI CTNAG 4 cut(s) 312, 529, 927, 952
DpnI GATC 7 cut(s) 15, 23, 333, 350, 831, 1256, 1320
DpnII GATC 7 cut(s) 13, 21, 331, 348, 829, 1254, 1318
EaeI YGGCCR 1 cut(s) 1225
Eco47I GGWCC 2 cut(s) 220, 401
Eco57I CTGAAG 1 cut(s) 434
Eco91I GGTNACC 1 cut(s) 1127
EcoO109I RGGNCCY 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 1127
EcoRII CCWGG 4 cut(s) 216, 257, 361, 1202
EcoT22I ATGCAT 3 cut(s) 469, 901, 905
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 9 cut(s) 22, 471, 682, 903, 946, 1136, 1141, 1215, 1315
FalI AAGNNNNNCTT 6 cut(s) 784, 816, 972, 1004, 1089, 1121
FaqI GGGAC 2 cut(s) 276, 492
FatI CATG 9 cut(s) 18, 467, 678, 899, 942, 1132, 1137, 1211, 1311
FauI CCCGC 1 cut(s) 203
FauNDI CATATG 1 cut(s) 211
FbaI TGATCA 1 cut(s) 1254
Fnu4HI GCNGC 4 cut(s) 287, 846, 1281, 1308
FokI GGATG 3 cut(s) 512, 569, 1339
Fsp4HI GCNGC 4 cut(s) 287, 846, 1281, 1308
FspBI CTAG 2 cut(s) 372, 605
GluI GCNGC 4 cut(s) 287, 846, 1281, 1308
GsuI CTGGAG 2 cut(s) 375, 1225
HaeIII GGCC 5 cut(s) 169, 216, 360, 1227, 1272
HapII CCGG 4 cut(s) 165, 170, 201, 516
Hin1II CATG 9 cut(s) 22, 471, 682, 903, 946, 1136, 1141, 1215, 1315
HinfI GANTC 9 cut(s) 67, 308, 480, 653, 733, 770, 884, 1177, 1199
HpaII CCGG 4 cut(s) 165, 170, 201, 516
HphI GGTGA 5 cut(s) 38, 184, 247, 662, 1139
Hpy166II GTNNAC 2 cut(s) 1160, 1195
Hpy188I TCNGA 8 cut(s) 159, 207, 315, 532, 829, 928, 998, 1182
Hpy188III TCNNGA 1 cut(s) 452
Hpy8I GTNNAC 2 cut(s) 1160, 1195
HpyAV CCTTC 2 cut(s) 993, 1115
HpyCH4III ACNGT 5 cut(s) 837, 1014, 1028, 1124, 1192
HpyCH4IV ACGT 1 cut(s) 252
HpyF10VI GCNNNNNNNGC 3 cut(s) 366, 601, 905
HpyF3I CTNAG 4 cut(s) 312, 529, 927, 952
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 9 cut(s) 22, 471, 682, 903, 946, 1136, 1141, 1215, 1315
KpnI GGTACC 2 cut(s) 50, 63
Ksp22I TGATCA 1 cut(s) 1254
Kzo9I GATC 7 cut(s) 13, 21, 331, 348, 829, 1254, 1318
LmnI GCTCC 1 cut(s) 203
Lsp1109I GCAGC 3 cut(s) 298, 832, 1294
LweI GCATC 4 cut(s) 490, 886, 907, 973
MaeI CTAG 2 cut(s) 372, 605
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 6 cut(s) 253, 650, 763, 796, 1127, 1331
MalI GATC 7 cut(s) 15, 23, 333, 350, 831, 1256, 1320
MbiI CCGCTC 1 cut(s) 198
MboI GATC 7 cut(s) 13, 21, 331, 348, 829, 1254, 1318
MboII GAAGA 3 cut(s) 187, 196, 290
MfeI CAATTG 1 cut(s) 1218
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 3 cut(s) 164, 1007, 1114
MlsI TGGCCA 1 cut(s) 1227
MluCI AATT 9 cut(s) 131, 566, 579, 587, 696, 1083, 1143, 1183, 1218
MluNI TGGCCA 1 cut(s) 1227
MlyI GAGTC 6 cut(s) 61, 317, 474, 647, 1171, 1208
Mox20I TGGCCA 1 cut(s) 1227
Mph1103I ATGCAT 3 cut(s) 469, 901, 905
MscI TGGCCA 1 cut(s) 1227
MseI TTAA 1 cut(s) 8
MslI CAYNNNNRTG 3 cut(s) 671, 782, 1165
Msp20I TGGCCA 1 cut(s) 1227
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 4 cut(s) 165, 170, 201, 516
MspR9I CCNGG 5 cut(s) 166, 218, 259, 363, 1204
MunI CAATTG 1 cut(s) 1218
Mva1269I GAATGC 1 cut(s) 793
MvaI CCWGG 4 cut(s) 218, 259, 363, 1204
MwoI GCNNNNNNNGC 3 cut(s) 366, 601, 905
NciI CCSGG 1 cut(s) 166
NdeI CATATG 1 cut(s) 211
NdeII GATC 7 cut(s) 13, 21, 331, 348, 829, 1254, 1318
NlaIII CATG 9 cut(s) 22, 471, 682, 903, 946, 1136, 1141, 1215, 1315
NlaIV GGNNCC 7 cut(s) 48, 61, 222, 320, 514, 758, 1006
NmuCI GTSAC 3 cut(s) 253, 650, 1127
NsiI ATGCAT 3 cut(s) 469, 901, 905
NspI RCATGY 3 cut(s) 903, 946, 1141
PaeI GCATGC 1 cut(s) 903
PciI ACATGT 1 cut(s) 1137
PctI GAATGC 1 cut(s) 793
PfeI GAWTC 3 cut(s) 733, 770, 884
PflFI GACNNNGTC 1 cut(s) 1213
PfoI TCCNGGA 1 cut(s) 1202
PkrI GCNGC 4 cut(s) 288, 847, 1282, 1309
PleI GAGTC 6 cut(s) 61, 316, 474, 647, 1171, 1207
PpsI GAGTC 6 cut(s) 61, 316, 474, 647, 1171, 1207
PscI ACATGT 1 cut(s) 1137
PsiI TTATAA 1 cut(s) 936
Psp6I CCWGG 4 cut(s) 216, 257, 361, 1202
PspEI GGTNACC 1 cut(s) 1127
PspGI CCWGG 4 cut(s) 216, 257, 361, 1202
PspN4I GGNNCC 7 cut(s) 48, 61, 222, 320, 514, 758, 1006
PspPI GGNCC 4 cut(s) 167, 220, 359, 401
PstI CTGCAG 1 cut(s) 1237
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 1 cut(s) 331
PsyI GACNNNGTC 1 cut(s) 1213
PvuII CAGCTG 1 cut(s) 289
RsaI GTAC 4 cut(s) 48, 61, 1025, 1159
RsaNI GTAC 4 cut(s) 47, 60, 1024, 1158
RseI CAYNNNNRTG 3 cut(s) 671, 782, 1165
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 4 cut(s) 287, 846, 1281, 1308
Sau3AI GATC 7 cut(s) 13, 21, 331, 348, 829, 1254, 1318
Sau96I GGNCC 4 cut(s) 167, 220, 359, 401
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 6 cut(s) 61, 317, 474, 647, 1171, 1208
ScrFI CCNGG 5 cut(s) 166, 218, 259, 363, 1204
SduI GDGCHC 3 cut(s) 164, 1007, 1114
SfaNI GCATC 4 cut(s) 490, 886, 907, 973
SfcI CTRYAG 1 cut(s) 1233
SinI GGWCC 2 cut(s) 220, 401
SmiMI CAYNNNNRTG 3 cut(s) 671, 782, 1165
SphI GCATGC 1 cut(s) 903
Sse9I AATT 9 cut(s) 131, 566, 579, 587, 696, 1083, 1143, 1183, 1218
SsiI CCGC 2 cut(s) 196, 1280
SspMI CTAG 2 cut(s) 372, 605
StyD4I CCNGG 5 cut(s) 164, 216, 257, 361, 1202
TaaI ACNGT 5 cut(s) 837, 1014, 1028, 1124, 1192
TaiI ACGT 1 cut(s) 255
TaqI TCGA 3 cut(s) 65, 306, 1321
TasI AATT 9 cut(s) 131, 566, 579, 587, 696, 1083, 1143, 1183, 1218
TatI WGTACW 1 cut(s) 1023
TauI GCSGC 1 cut(s) 1283
TfiI GAWTC 3 cut(s) 733, 770, 884
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 2 cut(s) 358, 1129
TseFI GTSAC 3 cut(s) 253, 650, 1127
TseI GCWGC 3 cut(s) 286, 845, 1307
Tsp45I GTSAC 3 cut(s) 253, 650, 1127
TspDTI ATGAA 7 cut(s) 99, 144, 291, 600, 723, 746, 800
TspRI CASTG 2 cut(s) 358, 1129
Tth111I GACNNNGTC 1 cut(s) 1213
VpaK11BI GGWCC 2 cut(s) 220, 401
XapI RAATTY 6 cut(s) 131, 566, 579, 587, 1083, 1183
XceI RCATGY 3 cut(s) 903, 946, 1141
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XspI CTAG 2 cut(s) 372, 605
ZrmI AGTACT 1 cut(s) 1025
Zsp2I ATGCAT 3 cut(s) 469, 901, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.