Rh4AG085000

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000839
Physical Location & Seq
Forward (+)
17773101 .. 17778188
5088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG085000.1

Sequence Viewer

Length: 1383 bp
ATGTGGACTACCTGGAAGATGGGTAGTAAGGTGAGGGCTGTAGATTTCCAGATCTCTGCCAGCATTACTACAACCAACATTACGATCCTACCGGGTTATTCCGGTGACCTTCCTTTTGCACTGGAATCCGGATATATCGGTGTTGGTGACAATGAAGAAGTGCAGCTATTTTACGTTTTCGTTGAGTCTGAGAGAGCTCCTGCAGACGATCCTCTGTTGCTATGGTTCAATGGAGGCCCAGGTTGCTCTGGTCTTGCTGGATTCTTCTTTGAGAGTGGTCCAGTAACTTTTAAGTTTGGAAATTACAATGGGAGCCTACCAGCAGTTCTTGACAATCCATTTCGATGGACAAAGGACGTCAGCATGATATACATAGATGCACCTGTTGGTGCTGGATTCTCCTACTCAAAAACTCAAAGCGGTTATGTTATGGATGACTATAAATATGTTGCACAAAGCTATGAATTTCTGCAGAAGTGGCTGGAGGCACACCCTGCATATTTGGAAAATGTACTTTACATTGGTGGTGACTCCTTCTCTGGCATTATTGTGCCAATGATTGTTCAACAAATATTGGATGGTAATGAAAATGGAACTTTGCCTCTACTGAATCTCAAGGGATATGTAATTGGGAACCCAGTAACGGATTCATTCATTGATGCAAATGCAAGAATTCCTTATGCCCATCGGCTAACTCTTATATCAGATCAACTGTATCGGGCGGCGAAAACAAGTTGCAGTGGAGATTACGTAAACGTAGATTCAAGCAATGACGCATGCATTTCAGATATCGATGCTATTGATGCACTCATAAATGACGTAAATACCTTACACGTCTTGGAACCAGTGTGTAGTGAAGGCACTGCCCGAAGATATCTCAAAGATTTGTCCGAAGGTTTACTAAGTTCAGCAACTACTGGTCCTGCATATTGGTGTAGGAGTTATAATCATATGCTCGCCTACAATTGGGCAAACCACCTAAGTGTTCAAGATGCTCTTGGCGTCCGAAGGGGCACAAAAAGATTTTGGCAATACTGTAATTCAACCCTGGCGTACACTCAAGAAGTCACTAGTGTTATTAGTCTTCATCAAAGTTTCACGGAGGTTGGCCTACGTGCTCTGATCTACAGTGGTGATCACGACATGACAATTCCTCACATTGCTACACAAAAATGGATAAACTTTCTCAATATGACTCTGAATGAGACCTGGAGGGCATGGTCAGTTGATAACCAAACTGCAGGGTACACAAAGAAGTTTGTAAATGAAGCTTTCAATTTGACGTTTGCAACTGTAAAGGGAGGAGGCCACATTGCTGCAGAGTACAAGGTCAAAGAATGCTCTGCAATGATTGATAGATGGATGGCTCAGTACCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

460

Amino Acids

51.19

Weight (kDa)

5.01

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 31 - 456 2.2e-115 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 945
AatII GACGTC 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 128
AciI CCGC 2 cut(s) 420, 722
AclWI GGATC 2 cut(s) 79, 203
AcsI RAATTY 2 cut(s) 464, 672
AcyI GRCGYC 2 cut(s) 357, 1002
AfaI GTAC 5 cut(s) 513, 1055, 1247, 1325, 1373
AfiI CCNNNNNNNGG 2 cut(s) 643, 966
AflIII ACRYGT 1 cut(s) 832
AgsI TTSAA 6 cut(s) 229, 566, 765, 989, 1044, 1276
AhlI ACTAGT 1 cut(s) 1070
AjiI CACGTC 1 cut(s) 835
AjnI CCWGG 4 cut(s) 11, 238, 1047, 1208
AleI CACNNNNGTG 1 cut(s) 981
AluBI AGCT 4 cut(s) 166, 197, 459, 1271
AluI AGCT 4 cut(s) 166, 197, 459, 1271
Alw21I GWGCWC 2 cut(s) 199, 1120
Alw26I GTCTC 1 cut(s) 1199
AlwI GGATC 2 cut(s) 79, 203
Aor13HI TCCGGA 1 cut(s) 128
AoxI GGCC 3 cut(s) 235, 1108, 1306
ApeKI GCWGC 2 cut(s) 163, 1316
ApoI RAATTY 2 cut(s) 464, 672
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AspS9I GGNCC 3 cut(s) 236, 278, 920
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 5 cut(s) 43, 116, 158, 539, 1145
AvaII GGWCC 2 cut(s) 278, 920
BaeGI GKGCMC 1 cut(s) 1016
BanII GRGCYC 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 1076
Bbv12I GWGCWC 2 cut(s) 199, 1120
BbvI GCAGC 2 cut(s) 175, 1303
BccI CCATC 6 cut(s) 13, 339, 572, 693, 1353, 1357
BciT130I CCWGG 4 cut(s) 13, 240, 1049, 1210
BclI TGATCA 1 cut(s) 1135
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 1199
BcuI ACTAGT 1 cut(s) 1070
BfaI CTAG 1 cut(s) 1071
BfmI CTRYAG 6 cut(s) 39, 201, 470, 1126, 1239, 1317
BglII AGATCT 1 cut(s) 51
BisI GCNGC 3 cut(s) 164, 723, 1317
BlsI GCNGC 3 cut(s) 165, 724, 1318
Bme1390I CCNGG 5 cut(s) 13, 93, 240, 1049, 1210
Bme18I GGWCC 2 cut(s) 278, 920
BmgBI CACGTC 1 cut(s) 835
BmgT120I GGNCC 3 cut(s) 236, 278, 920
BmiI GGNNCC 3 cut(s) 314, 635, 843
BmrFI CCNGG 5 cut(s) 13, 93, 240, 1049, 1210
BmrI ACTGGG 1 cut(s) 632
BmsI GCATC 5 cut(s) 367, 649, 784, 793, 982
BmuI ACTGGG 1 cut(s) 632
BpiI GAAGAC 1 cut(s) 1076
BpmI CTGGAG 2 cut(s) 503, 1231
BpuEI CTTGAG 2 cut(s) 599, 1044
BpuMI CCSGG 1 cut(s) 93
Bsa29I ATCGAT 1 cut(s) 792
BsaAI YACGTR 2 cut(s) 751, 1115
BsaHI GRCGYC 2 cut(s) 357, 1002
BsaI GGTCTC 1 cut(s) 1199
BsaJI CCNNGG 2 cut(s) 238, 1047
BsaWI WCCGGW 2 cut(s) 101, 128
BsaXI ACNNNNNCTCC 2 cut(s) 735, 765
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 966
Bse1I ACTGG 5 cut(s) 126, 281, 638, 845, 922
Bse3DI GCAATG 4 cut(s) 775, 1158, 1311, 1353
BseAI TCCGGA 1 cut(s) 128
BseBI CCWGG 4 cut(s) 13, 240, 1049, 1210
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 2 cut(s) 238, 1047
BseGI GGATG 3 cut(s) 439, 583, 1368
BseLI CCNNNNNNNGG 2 cut(s) 643, 966
BseMI GCAATG 4 cut(s) 775, 1158, 1311, 1353
BseMII CTCAG 2 cut(s) 180, 1382
BseNI ACTGG 5 cut(s) 126, 281, 638, 845, 922
BseRI GAGGAG 1 cut(s) 1317
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 2 cut(s) 175, 1303
BsgI GTGCAG 1 cut(s) 182
BshFI GGCC 3 cut(s) 237, 1110, 1308
BshVI ATCGAT 1 cut(s) 792
BsiHKAI GWGCWC 2 cut(s) 199, 1120
BsiSI CCGG 3 cut(s) 92, 102, 129
BslI CCNNNNNNNGG 2 cut(s) 643, 966
BsmAI GTCTC 1 cut(s) 1199
BsmI GAATGC 1 cut(s) 1343
BsnI GGCC 3 cut(s) 237, 1110, 1308
Bso31I GGTCTC 1 cut(s) 1199
Bsp1286I GDGCHC 3 cut(s) 199, 1016, 1120
Bsp13I TCCGGA 1 cut(s) 128
Bsp143I GATC 6 cut(s) 51, 84, 208, 706, 1122, 1135
BspACI CCGC 2 cut(s) 420, 722
BspANI GGCC 3 cut(s) 237, 1110, 1308
BspCNI CTCAG 2 cut(s) 181, 1381
BspDI ATCGAT 1 cut(s) 792
BspEI TCCGGA 1 cut(s) 128
BspLI GGNNCC 3 cut(s) 314, 635, 843
BspMAI CTGCAG 4 cut(s) 205, 474, 1243, 1321
BspPI GGATC 2 cut(s) 79, 203
BspTNI GGTCTC 1 cut(s) 1199
BsrDI GCAATG 4 cut(s) 775, 1158, 1311, 1353
BsrI ACTGG 5 cut(s) 126, 281, 638, 845, 922
BssECI CCNNGG 2 cut(s) 238, 1047
BssMI GATC 6 cut(s) 51, 84, 208, 706, 1122, 1135
BssNI GRCGYC 2 cut(s) 357, 1002
Bst2UI CCWGG 4 cut(s) 13, 240, 1049, 1210
Bst4CI ACNGT 4 cut(s) 714, 1037, 1130, 1294
BstACI GRCGYC 2 cut(s) 357, 1002
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBAI YACGTR 2 cut(s) 751, 1115
BstC8I GCNNGC 3 cut(s) 61, 778, 957
BstDEI CTNAG 4 cut(s) 189, 902, 980, 1368
BstEII GGTNACC 1 cut(s) 104
BstF5I GGATG 3 cut(s) 439, 583, 1368
BstKTI GATC 6 cut(s) 54, 87, 211, 709, 1125, 1138
BstMAI GTCTC 1 cut(s) 1199
BstMBI GATC 6 cut(s) 51, 84, 208, 706, 1122, 1135
BstMWI GCNNNNNNNGC 4 cut(s) 243, 478, 494, 803
BstNI CCWGG 4 cut(s) 13, 240, 1049, 1210
BstNSI RCATGY 1 cut(s) 780
BstPI GGTNACC 1 cut(s) 104
BstSCI CCNGG 5 cut(s) 11, 91, 238, 1047, 1208
BstSFI CTRYAG 6 cut(s) 39, 201, 470, 1126, 1239, 1317
BstSLI GKGCMC 1 cut(s) 1016
BstSNI TACGTA 1 cut(s) 751
BstV1I GCAGC 2 cut(s) 175, 1303
BstV2I GAAGAC 1 cut(s) 1076
BstX2I RGATCY 1 cut(s) 51
BstXI CCANNNNNNTGG 1 cut(s) 345
BstYI RGATCY 1 cut(s) 51
Bsu15I ATCGAT 1 cut(s) 792
BsuRI GGCC 3 cut(s) 237, 1110, 1308
BsuTUI ATCGAT 1 cut(s) 792
BtrI CACGTC 1 cut(s) 835
BtsCI GGATG 3 cut(s) 439, 583, 1368
BtsI GCAGTG 2 cut(s) 745, 861
BtsIMutI CAGTG 5 cut(s) 119, 745, 852, 861, 1135
Cac8I GCNNGC 3 cut(s) 61, 778, 957
Cfr13I GGNCC 3 cut(s) 236, 278, 920
ClaI ATCGAT 1 cut(s) 792
CseI GACGC 2 cut(s) 782, 991
Csp6I GTAC 5 cut(s) 512, 1054, 1246, 1324, 1372
CviAII CATG 4 cut(s) 364, 777, 1144, 1218
CviQI GTAC 5 cut(s) 512, 1054, 1246, 1324, 1372
DdeI CTNAG 4 cut(s) 189, 902, 980, 1368
DpnI GATC 6 cut(s) 53, 86, 210, 708, 1124, 1137
DpnII GATC 6 cut(s) 51, 84, 208, 706, 1122, 1135
Ecl136II GAGCTC 1 cut(s) 197
Eco105I TACGTA 1 cut(s) 751
Eco24I GRGCYC 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 1199
Eco32I GATATC 2 cut(s) 790, 875
Eco47I GGWCC 2 cut(s) 278, 920
Eco53kI GAGCTC 1 cut(s) 197
Eco91I GGTNACC 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 104
EcoRI GAATTC 1 cut(s) 672
EcoRII CCWGG 4 cut(s) 11, 238, 1047, 1208
EcoRV GATATC 2 cut(s) 790, 875
EcoT22I ATGCAT 1 cut(s) 782
EcoT38I GRGCYC 1 cut(s) 199
FaeI CATG 4 cut(s) 367, 780, 1147, 1221
FalI AAGNNNNNCTT 4 cut(s) 661, 693, 981, 1013
FatI CATG 4 cut(s) 363, 776, 1143, 1217
FauNDI CATATG 1 cut(s) 951
FbaI TGATCA 1 cut(s) 1135
Fnu4HI GCNGC 3 cut(s) 164, 723, 1317
FokI GGATG 3 cut(s) 446, 590, 1375
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 3 cut(s) 164, 723, 1317
FspBI CTAG 1 cut(s) 1071
GluI GCNGC 3 cut(s) 164, 723, 1317
GsuI CTGGAG 2 cut(s) 503, 1231
HaeIII GGCC 3 cut(s) 237, 1110, 1308
HapII CCGG 3 cut(s) 92, 102, 129
HgaI GACGC 2 cut(s) 782, 991
Hin1I GRCGYC 2 cut(s) 357, 1002
Hin1II CATG 4 cut(s) 367, 780, 1147, 1221
HindIII AAGCTT 1 cut(s) 1269
HinfI GANTC 9 cut(s) 125, 185, 261, 396, 530, 610, 647, 761, 1195
HpaII CCGG 3 cut(s) 92, 102, 129
HphI GGTGA 5 cut(s) 43, 116, 158, 539, 1145
Hpy166II GTNNAC 5 cut(s) 6, 754, 899, 1056, 1248
Hpy188I TCNGA 7 cut(s) 190, 706, 787, 892, 1007, 1122, 1200
Hpy188III TCNNGA 6 cut(s) 49, 129, 329, 989, 1061, 1139
Hpy8I GTNNAC 5 cut(s) 6, 754, 899, 1056, 1248
HpyAV CCTTC 5 cut(s) 119, 544, 851, 887, 1002
HpyCH4III ACNGT 4 cut(s) 714, 1037, 1130, 1294
HpyCH4IV ACGT 8 cut(s) 174, 357, 750, 756, 819, 834, 1114, 1283
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 478, 494, 803
HpyF3I CTNAG 4 cut(s) 189, 902, 980, 1368
HpySE526I ACGT 8 cut(s) 174, 357, 750, 756, 819, 834, 1114, 1283
Hsp92I GRCGYC 2 cut(s) 357, 1002
Hsp92II CATG 4 cut(s) 367, 780, 1147, 1221
Kpn2I TCCGGA 1 cut(s) 128
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 6 cut(s) 51, 84, 208, 706, 1122, 1135
LmnI GCTCC 2 cut(s) 202, 312
Lsp1109I GCAGC 2 cut(s) 175, 1303
LweI GCATC 5 cut(s) 367, 649, 784, 793, 982
MaeI CTAG 1 cut(s) 1071
MaeII ACGT 8 cut(s) 174, 357, 750, 756, 819, 834, 1114, 1283
MaeIII GTNAC 6 cut(s) 104, 146, 283, 527, 640, 1066
MalI GATC 6 cut(s) 53, 86, 210, 708, 1124, 1137
MboI GATC 6 cut(s) 51, 84, 208, 706, 1122, 1135
MboII GAAGA 5 cut(s) 28, 167, 256, 882, 1076
MfeI CAATTG 1 cut(s) 964
MflI RGATCY 1 cut(s) 51
MhlI GDGCHC 3 cut(s) 199, 1016, 1120
MluCI AATT 8 cut(s) 301, 464, 627, 672, 964, 1039, 1149, 1276
MlyI GAGTC 3 cut(s) 194, 524, 1189
Mph1103I ATGCAT 1 cut(s) 782
MroI TCCGGA 1 cut(s) 128
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 5 cut(s) 343, 548, 931, 981, 1171
MspI CCGG 3 cut(s) 92, 102, 129
MspR9I CCNGG 5 cut(s) 13, 93, 240, 1049, 1210
MunI CAATTG 1 cut(s) 964
Mva1269I GAATGC 1 cut(s) 1343
MvaI CCWGG 4 cut(s) 13, 240, 1049, 1210
MwoI GCNNNNNNNGC 4 cut(s) 243, 478, 494, 803
NciI CCSGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 951
NdeII GATC 6 cut(s) 51, 84, 208, 706, 1122, 1135
NlaIII CATG 4 cut(s) 367, 780, 1147, 1221
NlaIV GGNNCC 3 cut(s) 314, 635, 843
NmuCI GTSAC 4 cut(s) 104, 146, 527, 1066
NsiI ATGCAT 1 cut(s) 782
NspI RCATGY 1 cut(s) 780
OliI CACNNNNGTG 1 cut(s) 981
PaeI GCATGC 1 cut(s) 780
PctI GAATGC 1 cut(s) 1343
PfeI GAWTC 6 cut(s) 125, 261, 396, 610, 647, 761
PkrI GCNGC 3 cut(s) 165, 724, 1318
PleI GAGTC 3 cut(s) 193, 524, 1189
PpsI GAGTC 3 cut(s) 193, 524, 1189
Ppu21I YACGTR 2 cut(s) 751, 1115
PsiI TTATAA 1 cut(s) 945
Psp124BI GAGCTC 1 cut(s) 199
Psp6I CCWGG 4 cut(s) 11, 238, 1047, 1208
PspEI GGTNACC 1 cut(s) 104
PspGI CCWGG 4 cut(s) 11, 238, 1047, 1208
PspN4I GGNNCC 3 cut(s) 314, 635, 843
PspPI GGNCC 3 cut(s) 236, 278, 920
PstI CTGCAG 4 cut(s) 205, 474, 1243, 1321
PsuI RGATCY 1 cut(s) 51
RsaI GTAC 5 cut(s) 513, 1055, 1247, 1325, 1373
RsaNI GTAC 5 cut(s) 512, 1054, 1246, 1324, 1372
RseI CAYNNNNRTG 5 cut(s) 343, 548, 931, 981, 1171
SacI GAGCTC 1 cut(s) 199
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 3 cut(s) 164, 723, 1317
Sau3AI GATC 6 cut(s) 51, 84, 208, 706, 1122, 1135
Sau96I GGNCC 3 cut(s) 236, 278, 920
SchI GAGTC 3 cut(s) 194, 524, 1189
ScrFI CCNGG 5 cut(s) 13, 93, 240, 1049, 1210
SduI GDGCHC 3 cut(s) 199, 1016, 1120
SfaNI GCATC 5 cut(s) 367, 649, 784, 793, 982
SfcI CTRYAG 6 cut(s) 39, 201, 470, 1126, 1239, 1317
SinI GGWCC 2 cut(s) 278, 920
SmiMI CAYNNNNRTG 5 cut(s) 343, 548, 931, 981, 1171
SmlI CTYRAG 2 cut(s) 614, 1059
SmoI CTYRAG 2 cut(s) 614, 1059
SnaBI TACGTA 1 cut(s) 751
SpeI ACTAGT 1 cut(s) 1070
SphI GCATGC 1 cut(s) 780
Sse9I AATT 8 cut(s) 301, 464, 627, 672, 964, 1039, 1149, 1276
SsiI CCGC 2 cut(s) 420, 722
SspI AATATT 1 cut(s) 573
SspMI CTAG 1 cut(s) 1071
SstI GAGCTC 1 cut(s) 199
StyD4I CCNGG 5 cut(s) 11, 91, 238, 1047, 1208
TaaI ACNGT 4 cut(s) 714, 1037, 1130, 1294
TaiI ACGT 8 cut(s) 177, 360, 753, 759, 822, 837, 1117, 1286
TaqI TCGA 2 cut(s) 343, 792
TasI AATT 8 cut(s) 301, 464, 627, 672, 964, 1039, 1149, 1276
TatI WGTACW 2 cut(s) 511, 1323
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 6 cut(s) 125, 261, 396, 610, 647, 761
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 5 cut(s) 126, 745, 852, 868, 1135
TseFI GTSAC 4 cut(s) 104, 146, 527, 1066
TseI GCWGC 2 cut(s) 163, 1316
Tsp45I GTSAC 4 cut(s) 104, 146, 527, 1066
TspDTI ATGAA 7 cut(s) 168, 477, 600, 639, 643, 1076, 1281
TspGWI ACGGA 2 cut(s) 659, 1115
TspRI CASTG 5 cut(s) 126, 745, 852, 868, 1135
VpaK11BI GGWCC 2 cut(s) 278, 920
XapI RAATTY 2 cut(s) 464, 672
XceI RCATGY 1 cut(s) 780
XspI CTAG 1 cut(s) 1071
ZraI GACGTC 1 cut(s) 358
Zsp2I ATGCAT 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.