FvH4_4g06510

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000839
Physical Location & Seq
Forward (+)
5790939 .. 5795541
4603 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06510.t3

Sequence Viewer

Length: 1422 bp
ATGAATATGTTCATGGGTTTGCGTCAACTTGTCACATTATTGAGTTTAATCACTTTAACTTCAGCTTCAAACATCACTACGAAAATTGTTACCACTCTACCGGGTTACTCTGGAGATCTTCCTTTCACAATGGAAACCGGATATATCGGTGTAGGTGACAATGAGGAAGTGCAGCTATTTTACGTTTTTGTGGATTCTCAGAGAAGCCCTGCACAAGATCCACTTGTGTTACGGTTTGGTGCAGGTCCAGGCTGCTCTGGTCTCCATGGCTTTTTCTTTGAGAATGGTCCATTATCTTTCAATTCTGCACATTACAATGGAAGCCTACCAACACTTGAGGACAACCCTTATTCATGGACAGAGGCACTCAACATCATATTCTTGGATGCACCAGCTGGTGCTGGATTCTCCTATTCAACAACCCAAGAAGGTTTCTATGTGGACGAGTATAAATATGCAGAACAAGGTTATGAATTTCTCCAGAAGTGGCTGGAGGCACACCCTGCATATGTGGAAAATCAACTCTACATCAGTGGTGAATCCTTCAGTGGGTTGATAGTTCCAATGATTGTACAAAAGATAGTGGATGGTAATGAAAATGGAAGTGTGCCTCTAATGAACATCAAGGGATATCTGCTTCAGAACCCAGTCACGGATTCAGTTATTGATGGTAATGCAATAATCCCATATGCCCACCGGCTGACTCTTGTATCGGATCAACTTTATGAGGCAGCAAAAACAAGTTGCAATGGTGAATACGTGAATGTAAATTCAAGCAATGCTGCATGCATTGCTGATATCGAGGCTATTGATCAGCTCATCAAGGATATAAATCCTGTCAATATCTTGGAACCAGACTGTAGTACTTCATTGCCGAGGTCAAAGAAGATAAGCACAGCTCGAAGATATCTTAAAGAGTTATACAAAGGTTTACTAAGTTCACCAACCAATGGTCCTGCACTTTGGTGTCGGGAGTACAATTACATGCTTCTGTACATCTGGGCAAACACCAAAGCTGTCCGAGATGCTCTTGGTGTCCGAACGGGTACAAAATTGTTGTGGAAACCATGTAATACCACATTGACGGGTTACACTTATGTAGTGACCAGTTCTCTCAGTTATCATCTCAATCTCTCAAAAAAGGCTGACCTGCGTGCTTTGATATACAGTGGTGACCATGACATGTCTGTTCCTCATATTGGTACACAAGCATGGATAAATTTGCTGAATTTGACTGTAGATGAGTCCTGGAGGACATGGTCCCTTGATGGACAAACTAAAGGATACACCAAGAAGTTAATAAATGATTACATCACCTTGATATTTGCTACTGTAAAGGCAACAGGCCACCTTGCTGCAGAGTACCAGCCAAAGGAAGTTGCTAGCCTGGTTGGTAGATGGATGGCTTACTATCCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

474

Amino Acids

52.52

Weight (kDa)

5.27

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 34 - 468 2e-110 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 233, 1158
AccB7I CCANNNNNTGG 1 cut(s) 950
AclWI GGATC 2 cut(s) 212, 723
AcsI RAATTY 4 cut(s) 473, 769, 1219, 1228
AcuI CTGAAG 3 cut(s) 45, 529, 623
AfaI GTAC 7 cut(s) 573, 865, 977, 995, 1048, 1204, 1364
AfiI CCNNNNNNNGG 5 cut(s) 549, 652, 950, 1199, 1372
AflIII ACRYGT 1 cut(s) 1182
AgsI TTSAA 4 cut(s) 69, 301, 417, 774
AjnI CCWGG 3 cut(s) 247, 1247, 1386
AleI CACNNNNGTG 1 cut(s) 964
AluBI AGCT 6 cut(s) 65, 175, 395, 817, 899, 1016
AluI AGCT 6 cut(s) 65, 175, 395, 817, 899, 1016
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 2 cut(s) 212, 723
AoxI GGCC 1 cut(s) 1345
ApeKI GCWGC 5 cut(s) 172, 252, 731, 782, 1355
ApoI RAATTY 4 cut(s) 473, 769, 1219, 1228
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 4 cut(s) 245, 287, 953, 1260
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 6 cut(s) 167, 548, 764, 933, 1184, 1306
AsuNHI GCTAGC 1 cut(s) 1382
AvaII GGWCC 4 cut(s) 245, 287, 953, 1260
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BbvI GCAGC 5 cut(s) 184, 239, 743, 769, 1342
BccI CCATC 5 cut(s) 581, 662, 1262, 1392, 1396
BciT130I CCWGG 3 cut(s) 249, 1249, 1388
BciVI GTATCC 1 cut(s) 1277
BclI TGATCA 1 cut(s) 811
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 1 cut(s) 1383
BfmI CTRYAG 3 cut(s) 859, 1236, 1356
BfuAI ACCTGC 2 cut(s) 233, 1158
BfuI GTATCC 1 cut(s) 1277
BglII AGATCT 1 cut(s) 115
BisI GCNGC 5 cut(s) 173, 253, 732, 783, 1356
BlsI GCNGC 5 cut(s) 174, 254, 733, 784, 1357
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 4 cut(s) 102, 249, 1249, 1388
Bme18I GGWCC 4 cut(s) 245, 287, 953, 1260
BmgT120I GGNCC 4 cut(s) 245, 287, 953, 1260
BmiI GGNNCC 2 cut(s) 852, 1262
BmrFI CCNGG 4 cut(s) 102, 249, 1249, 1388
BmrI ACTGGG 1 cut(s) 641
BmsI GCATC 2 cut(s) 376, 1015
BmtI GCTAGC 1 cut(s) 1386
BmuI ACTGGG 1 cut(s) 641
BpmI CTGGAG 4 cut(s) 132, 464, 512, 1270
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 102
BsaAI YACGTR 1 cut(s) 760
BsaBI GATNNNNATC 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 2 cut(s) 265, 875
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 5 cut(s) 549, 652, 950, 1199, 1372
Bse118I RCCGGY 1 cut(s) 696
Bse1I ACTGG 2 cut(s) 647, 1107
Bse3DI GCAATG 4 cut(s) 754, 784, 789, 869
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 3 cut(s) 249, 1249, 1388
BseDI CCNNGG 2 cut(s) 265, 875
BseGI GGATG 3 cut(s) 391, 592, 1407
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 5 cut(s) 549, 652, 950, 1199, 1372
BseMI GCAATG 4 cut(s) 754, 784, 789, 869
BseMII CTCAG 2 cut(s) 212, 1129
BseNI ACTGG 2 cut(s) 647, 1107
BseXI GCAGC 5 cut(s) 184, 239, 743, 769, 1342
BsgI GTGCAG 5 cut(s) 191, 195, 261, 291, 942
BshFI GGCC 1 cut(s) 1347
BsiSI CCGG 3 cut(s) 101, 138, 697
BslFI GGGAC 1 cut(s) 1246
BslI CCNNNNNNNGG 5 cut(s) 549, 652, 950, 1199, 1372
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 1246
BsnI GGCC 1 cut(s) 1347
Bso31I GGTCTC 1 cut(s) 266
Bsp1407I TGTACA 2 cut(s) 571, 993
Bsp143I GATC 4 cut(s) 115, 217, 715, 811
Bsp19I CCATGG 1 cut(s) 265
BspANI GGCC 1 cut(s) 1347
BspCNI CTCAG 2 cut(s) 211, 1128
BspLI GGNNCC 2 cut(s) 852, 1262
BspMAI CTGCAG 1 cut(s) 1360
BspMI ACCTGC 2 cut(s) 233, 1158
BspOI GCTAGC 1 cut(s) 1386
BspPI GGATC 2 cut(s) 212, 723
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 4 cut(s) 754, 784, 789, 869
BsrFI RCCGGY 1 cut(s) 696
BsrGI TGTACA 2 cut(s) 571, 993
BsrI ACTGG 2 cut(s) 647, 1107
BssAI RCCGGY 1 cut(s) 696
BssECI CCNNGG 2 cut(s) 265, 875
BssMI GATC 4 cut(s) 115, 217, 715, 811
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 3 cut(s) 249, 1249, 1388
Bst4CI ACNGT 5 cut(s) 234, 860, 1169, 1237, 1333
BstAPI GCANNNNNTGC 2 cut(s) 503, 791
BstAUI TGTACA 2 cut(s) 571, 993
BstBAI YACGTR 1 cut(s) 760
BstC8I GCNNGC 3 cut(s) 787, 1155, 1384
BstDEI CTNAG 3 cut(s) 198, 935, 1115
BstDSI CCRYGG 1 cut(s) 265
BstEII GGTNACC 1 cut(s) 1172
BstF5I GGATG 3 cut(s) 391, 592, 1407
BstKTI GATC 4 cut(s) 118, 220, 718, 814
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 4 cut(s) 115, 217, 715, 811
BstMWI GCNNNNNNNGC 2 cut(s) 503, 791
BstNI CCWGG 3 cut(s) 249, 1249, 1388
BstNSI RCATGY 3 cut(s) 789, 988, 1186
BstPI GGTNACC 1 cut(s) 1172
BstSCI CCNGG 4 cut(s) 100, 247, 1247, 1386
BstSFI CTRYAG 3 cut(s) 859, 1236, 1356
BstV1I GCAGC 5 cut(s) 184, 239, 743, 769, 1342
BstX2I RGATCY 2 cut(s) 115, 217
BstYI RGATCY 2 cut(s) 115, 217
BsuI GTATCC 1 cut(s) 1277
BsuRI GGCC 1 cut(s) 1347
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 3 cut(s) 391, 592, 1407
BtsIMutI CAGTG 3 cut(s) 538, 553, 1174
BveI ACCTGC 2 cut(s) 233, 1158
Cac8I GCNNGC 3 cut(s) 787, 1155, 1384
Cfr10I RCCGGY 1 cut(s) 696
Cfr13I GGNCC 4 cut(s) 245, 287, 953, 1260
CseI GACGC 1 cut(s) 11
Csp6I GTAC 7 cut(s) 572, 864, 976, 994, 1047, 1203, 1363
CviQI GTAC 7 cut(s) 572, 864, 976, 994, 1047, 1203, 1363
DdeI CTNAG 3 cut(s) 198, 935, 1115
DpnI GATC 4 cut(s) 117, 219, 717, 813
DpnII GATC 4 cut(s) 115, 217, 715, 811
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 266
Eco32I GATATC 3 cut(s) 632, 799, 908
Eco47I GGWCC 4 cut(s) 245, 287, 953, 1260
Eco57I CTGAAG 3 cut(s) 45, 529, 623
Eco91I GGTNACC 1 cut(s) 1172
EcoO65I GGTNACC 1 cut(s) 1172
EcoRII CCWGG 3 cut(s) 247, 1247, 1386
EcoRV GATATC 3 cut(s) 632, 799, 908
EcoT14I CCWWGG 1 cut(s) 265
EcoT22I ATGCAT 1 cut(s) 791
ErhI CCWWGG 1 cut(s) 265
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FaqI GGGAC 1 cut(s) 1246
FauNDI CATATG 2 cut(s) 508, 688
FbaI TGATCA 1 cut(s) 811
Fnu4HI GCNGC 5 cut(s) 173, 253, 732, 783, 1356
FokI GGATG 3 cut(s) 398, 599, 1414
Fsp4HI GCNGC 5 cut(s) 173, 253, 732, 783, 1356
FspBI CTAG 1 cut(s) 1383
GluI GCNGC 5 cut(s) 173, 253, 732, 783, 1356
GsuI CTGGAG 4 cut(s) 132, 464, 512, 1270
HaeIII GGCC 1 cut(s) 1347
HapII CCGG 3 cut(s) 101, 138, 697
HgaI GACGC 1 cut(s) 11
HincII GTYRAC 1 cut(s) 26
HindII GTYRAC 1 cut(s) 26
HinfI GANTC 6 cut(s) 194, 405, 539, 656, 703, 1244
HpaII CCGG 3 cut(s) 101, 138, 697
HphI GGTGA 6 cut(s) 167, 548, 764, 933, 1184, 1306
Hpy166II GTNNAC 5 cut(s) 26, 442, 932, 941, 1205
Hpy188I TCNGA 5 cut(s) 201, 642, 715, 1022, 1040
Hpy188III TCNNGA 3 cut(s) 111, 481, 971
Hpy8I GTNNAC 5 cut(s) 26, 442, 932, 941, 1205
HpyAV CCTTC 2 cut(s) 422, 553
HpyCH4III ACNGT 5 cut(s) 234, 860, 1169, 1237, 1333
HpyCH4IV ACGT 2 cut(s) 183, 759
HpyF10VI GCNNNNNNNGC 2 cut(s) 503, 791
HpyF3I CTNAG 3 cut(s) 198, 935, 1115
HpySE526I ACGT 2 cut(s) 183, 759
Ksp22I TGATCA 1 cut(s) 811
Kzo9I GATC 4 cut(s) 115, 217, 715, 811
Lsp1109I GCAGC 5 cut(s) 184, 239, 743, 769, 1342
LweI GCATC 2 cut(s) 376, 1015
MaeI CTAG 1 cut(s) 1383
MaeII ACGT 2 cut(s) 183, 759
MaeIII GTNAC 9 cut(s) 31, 88, 104, 155, 228, 649, 1088, 1102, 1172
MalI GATC 4 cut(s) 117, 219, 717, 813
MboI GATC 4 cut(s) 115, 217, 715, 811
MboII GAAGA 3 cut(s) 110, 898, 915
MflI RGATCY 2 cut(s) 115, 217
MluCI AATT 8 cut(s) 84, 301, 473, 769, 979, 1052, 1219, 1228
MlyI GAGTC 2 cut(s) 697, 1253
Mph1103I ATGCAT 1 cut(s) 791
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 4 cut(s) 47, 56, 912, 1298
MslI CAYNNNNRTG 3 cut(s) 315, 964, 1210
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 3 cut(s) 101, 138, 697
MspR9I CCNGG 4 cut(s) 102, 249, 1249, 1388
MvaI CCWGG 3 cut(s) 249, 1249, 1388
MwoI GCNNNNNNNGC 2 cut(s) 503, 791
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 1 cut(s) 265
NdeI CATATG 2 cut(s) 508, 688
NdeII GATC 4 cut(s) 115, 217, 715, 811
NheI GCTAGC 1 cut(s) 1382
NlaIV GGNNCC 2 cut(s) 852, 1262
NmeAIII GCCGAG 1 cut(s) 900
NmuCI GTSAC 5 cut(s) 31, 155, 649, 1102, 1172
NsiI ATGCAT 1 cut(s) 791
NspI RCATGY 3 cut(s) 789, 988, 1186
OliI CACNNNNGTG 1 cut(s) 964
PaeI GCATGC 1 cut(s) 789
PciI ACATGT 1 cut(s) 1182
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 4 cut(s) 194, 405, 539, 656
PflFI GACNNNGTC 1 cut(s) 1258
PflMI CCANNNNNTGG 1 cut(s) 950
PfoI TCCNGGA 1 cut(s) 1247
PkrI GCNGC 5 cut(s) 174, 254, 733, 784, 1357
PleI GAGTC 2 cut(s) 697, 1252
PpsI GAGTC 2 cut(s) 697, 1252
Ppu21I YACGTR 1 cut(s) 760
PscI ACATGT 1 cut(s) 1182
Psp6I CCWGG 3 cut(s) 247, 1247, 1386
PspEI GGTNACC 1 cut(s) 1172
PspGI CCWGG 3 cut(s) 247, 1247, 1386
PspN4I GGNNCC 2 cut(s) 852, 1262
PspPI GGNCC 4 cut(s) 245, 287, 953, 1260
PstI CTGCAG 1 cut(s) 1360
PsuI RGATCY 2 cut(s) 115, 217
PsyI GACNNNGTC 1 cut(s) 1258
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 7 cut(s) 573, 865, 977, 995, 1048, 1204, 1364
RsaNI GTAC 7 cut(s) 572, 864, 976, 994, 1047, 1203, 1363
RseI CAYNNNNRTG 3 cut(s) 315, 964, 1210
SaqAI TTAA 4 cut(s) 47, 56, 912, 1298
SatI GCNGC 5 cut(s) 173, 253, 732, 783, 1356
Sau3AI GATC 4 cut(s) 115, 217, 715, 811
Sau96I GGNCC 4 cut(s) 245, 287, 953, 1260
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 2 cut(s) 697, 1253
ScrFI CCNGG 4 cut(s) 102, 249, 1249, 1388
SfaNI GCATC 2 cut(s) 376, 1015
SfcI CTRYAG 3 cut(s) 859, 1236, 1356
SinI GGWCC 4 cut(s) 245, 287, 953, 1260
SmiMI CAYNNNNRTG 3 cut(s) 315, 964, 1210
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SphI GCATGC 1 cut(s) 789
Sse9I AATT 8 cut(s) 84, 301, 473, 769, 979, 1052, 1219, 1228
SspMI CTAG 1 cut(s) 1383
StyD4I CCNGG 4 cut(s) 100, 247, 1247, 1386
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 5 cut(s) 234, 860, 1169, 1237, 1333
TaiI ACGT 2 cut(s) 186, 762
TaqI TCGA 2 cut(s) 801, 901
TasI AATT 8 cut(s) 84, 301, 473, 769, 979, 1052, 1219, 1228
TatI WGTACW 4 cut(s) 571, 863, 975, 993
TfiI GAWTC 4 cut(s) 194, 405, 539, 656
Tru1I TTAA 4 cut(s) 47, 56, 912, 1298
Tru9I TTAA 4 cut(s) 47, 56, 912, 1298
TscAI CASTG 3 cut(s) 538, 553, 1174
TseFI GTSAC 5 cut(s) 31, 155, 649, 1102, 1172
TseI GCWGC 5 cut(s) 172, 252, 731, 782, 1355
Tsp45I GTSAC 5 cut(s) 31, 155, 649, 1102, 1172
TspDTI ATGAA 6 cut(s) 17, 342, 486, 609, 632, 858
TspGWI ACGGA 1 cut(s) 668
TspRI CASTG 3 cut(s) 538, 553, 1174
Tth111I GACNNNGTC 1 cut(s) 1258
Van91I CCANNNNNTGG 1 cut(s) 950
VpaK11BI GGWCC 4 cut(s) 245, 287, 953, 1260
XapI RAATTY 4 cut(s) 473, 769, 1219, 1228
XceI RCATGY 3 cut(s) 789, 988, 1186
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 1383
ZrmI AGTACT 1 cut(s) 865
Zsp2I ATGCAT 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.