FvH4_4g07930

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000839
Physical Location & Seq
Forward (+)
7485565 .. 7489899
4335 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07930.t3

Sequence Viewer

Length: 1422 bp
ATGGCACCAGAAAGGCTCCAATGTCTTAAGAAATATATCGTGCCGATATTGTGTGTACATTTAGTTCTTTTCTCAAGTTTGTGTGGGATTGCTACCTCACAAACCATAGTCACCAGTTTACCTGGTTTTGAAGGGGACCTGCCCTTCACACTCGAAACTGGGTATGTGGGTGTGGGTGACTCAAACACCTCTCAATTGTTCTACTATTTTGTTGAGTCGCAGAGAAGCCCTGCCGAAGACCCTCTTATGCTTTGGTTCACTGGAGGCCCTGGCTGCTCTGTTCTCTCTGCCTTCTTTTATGAAAGTGGTCCACTATCTTTCACATATGTAGATTACAATGGAAGTCTACCCTCACTTCATTTGAATCCATACGCATGGACACAGGGCCTTAACATAATATATGTAGATTCACCTGTTGGCACCGGATTCTCTTACTCTACAACCCAAGAAGGTTACTATATCGATGACGAAGATGCCGTGGACCAGAGTGTTCAGTTTCTAAGAAAGTGGCTGATGGCTCATCCTCAATTTTTGAACAATATACTCTACATTGGTGGCGATTCATATTCTGGAATTCCTCTTCCTATCATAGTACAACAGCTTTATGATGGTAACAATGCTGGAGTGGAGCCCTATCTCAACCTTCGAGGGTATATACTTGGAAACCCGAAGACTGACGACTTCATTGATGTCAACTCTGTCAACCCCTTTGCACACAGGTTGACACTCATATCAGATGAACTTTATGAGTCTACCAAAGAGAGCTGCGGCGGTGACTACGTTAATGTAGATGATAACAACACAGTATGTGTGACAGATCTTGCAGCTATTGATCAGCTGCTTCTTCAAATTAATATTATGCATGTCTTGGAACCCAACTGTCAAGTGGCCTTACCAAACAACAGAGAAGAACCAGGAAGAAGATCAATCCGAGAACTACCAGAAAATCTACTCACAGAATCCACTCCTGCATTATGGTGCCGGTACTACAACTATGTGATCTTGGGTGTGTGGGCTAATGACCACGCTGTCCGCAAAGCTCTTAACGTCAGCGAGAACTCCATTGGTGTTTGGAAGAGGTGTAATATGAGCATAGCTTACACAAAGACTGTCACAAGTAGCGTTGAGTATCACAAGAATCTCAGTGACACAAACCTTCGAGCTCTTATATACAGTGGTGACCATGACATGTCTGTTCCACACATCGGCACCCAGGAGTGGATCCGTTATCTAAATTTGACAAACAGTGAGATTTGGCGGCCGTGGTATGTAGATGGTCAAGTAGCTGGATACTCAGAAAAGTTCTCAAACAATGACTACAGTCTGCAATATGCAACTGGTGCGGGTCATGTGGCTCCCGAGTACAAAGCTAAGGAATGTTATGCGATGATTGATCGGTGGCTCGCTTATTACCCTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

474

Amino Acids

53.25

Weight (kDa)

4.68

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 41 - 448 1e-108 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1028
Acc36I ACCTGC 1 cut(s) 147
AccB1I GGYRCC 4 cut(s) 4, 419, 978, 1208
AccI GTMKAC 2 cut(s) 346, 752
AciI CCGC 5 cut(s) 768, 771, 1033, 1258, 1343
AclWI GGATC 2 cut(s) 1216, 1229
AcoI YGGCCR 1 cut(s) 1259
AcsI RAATTY 2 cut(s) 573, 1234
AfaI GTAC 4 cut(s) 57, 594, 986, 1364
AfiI CCNNNNNNNGG 2 cut(s) 1205, 1218
AflII CTTAAG 1 cut(s) 26
AflIII ACRYGT 1 cut(s) 1188
AgsI TTSAA 4 cut(s) 131, 364, 535, 848
AjnI CCWGG 4 cut(s) 121, 268, 913, 1212
AluBI AGCT 9 cut(s) 601, 765, 827, 838, 1040, 1097, 1163, 1286, 1370
AluI AGCT 9 cut(s) 601, 765, 827, 838, 1040, 1097, 1163, 1286, 1370
Alw21I GWGCWC 1 cut(s) 1165
AlwI GGATC 2 cut(s) 1216, 1229
Ama87I CYCGRG 1 cut(s) 1358
AoxI GGCC 4 cut(s) 265, 385, 888, 1259
ApeKI GCWGC 4 cut(s) 273, 765, 824, 838
ApoI RAATTY 2 cut(s) 573, 1234
AseI ATTAAT 1 cut(s) 852
AspS9I GGNCC 5 cut(s) 136, 266, 308, 385, 481
AsuHPI GGTGA 5 cut(s) 103, 188, 402, 785, 1190
AvaI CYCGRG 1 cut(s) 1358
AvaII GGWCC 3 cut(s) 136, 308, 481
BamHI GGATCC 1 cut(s) 1221
BanI GGYRCC 4 cut(s) 4, 419, 978, 1208
BanII GRGCYC 2 cut(s) 633, 1165
BbsI GAAGAC 2 cut(s) 243, 677
Bbv12I GWGCWC 1 cut(s) 1165
BbvI GCAGC 4 cut(s) 260, 752, 825, 836
BccI CCATC 3 cut(s) 508, 602, 1268
BceAI ACGGC 2 cut(s) 461, 1246
BciT130I CCWGG 4 cut(s) 123, 270, 915, 1214
BciVI GTATCC 1 cut(s) 1283
BclI TGATCA 1 cut(s) 832
BfmI CTRYAG 1 cut(s) 1318
BfrI CTTAAG 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 147
BfuI GTATCC 1 cut(s) 1283
BglII AGATCT 1 cut(s) 817
BisI GCNGC 6 cut(s) 274, 766, 769, 825, 839, 1259
BlsI GCNGC 6 cut(s) 275, 767, 770, 826, 840, 1260
Bme1390I CCNGG 4 cut(s) 123, 270, 915, 1214
Bme18I GGWCC 3 cut(s) 136, 308, 481
BmeT110I CYCGRG 1 cut(s) 1358
BmgT120I GGNCC 5 cut(s) 136, 266, 308, 385, 481
BmrFI CCNGG 4 cut(s) 123, 270, 915, 1214
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 168
BoxI GACNNNNGTC 1 cut(s) 1320
BpiI GAAGAC 2 cut(s) 243, 677
BpmI CTGGAG 2 cut(s) 282, 642
Bpu10I CCTNAGC 1 cut(s) 1371
BpuEI CTTGAG 1 cut(s) 58
Bsa29I ATCGAT 1 cut(s) 462
BsaJI CCNNGG 4 cut(s) 268, 477, 1212, 1262
BsaWI WCCGGW 1 cut(s) 422
Bsc4I CCNNNNNNNGG 2 cut(s) 1205, 1218
Bse118I RCCGGY 1 cut(s) 981
Bse1I ACTGG 4 cut(s) 114, 163, 265, 1342
BseBI CCWGG 4 cut(s) 123, 270, 915, 1214
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 4 cut(s) 268, 477, 1212, 1262
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 2 cut(s) 1205, 1218
BseMII CTCAG 2 cut(s) 1156, 1308
BseNI ACTGG 4 cut(s) 114, 163, 265, 1342
BseX3I CGGCCG 1 cut(s) 1259
BseXI GCAGC 4 cut(s) 260, 752, 825, 836
Bsh1285I CGRYCG 1 cut(s) 1262
BshFI GGCC 4 cut(s) 267, 387, 890, 1261
BshNI GGYRCC 4 cut(s) 4, 419, 978, 1208
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 1262
BsiHKAI GWGCWC 1 cut(s) 1165
BsiHKCI CYCGRG 1 cut(s) 1358
BsiSI CCGG 2 cut(s) 423, 982
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 1205, 1218
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 4 cut(s) 267, 387, 890, 1261
BsoBI CYCGRG 1 cut(s) 1358
Bsp1286I GDGCHC 2 cut(s) 633, 1165
Bsp1407I TGTACA 1 cut(s) 55
Bsp143I GATC 6 cut(s) 817, 832, 923, 999, 1221, 1393
BspACI CCGC 5 cut(s) 768, 771, 1033, 1258, 1343
BspANI GGCC 4 cut(s) 267, 387, 890, 1261
BspCNI CTCAG 2 cut(s) 1155, 1307
BspDI ATCGAT 1 cut(s) 462
BspMI ACCTGC 1 cut(s) 147
BspPI GGATC 2 cut(s) 1216, 1229
BspT107I GGYRCC 4 cut(s) 4, 419, 978, 1208
BspTI CTTAAG 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 981
BsrGI TGTACA 1 cut(s) 55
BsrI ACTGG 4 cut(s) 114, 163, 265, 1342
BssAI RCCGGY 1 cut(s) 981
BssECI CCNNGG 4 cut(s) 268, 477, 1212, 1262
BssMI GATC 6 cut(s) 817, 832, 923, 999, 1221, 1393
Bst2UI CCWGG 4 cut(s) 123, 270, 915, 1214
Bst4CI ACNGT 6 cut(s) 805, 881, 1111, 1175, 1247, 1322
Bst6I CTCTTC 2 cut(s) 585, 1070
BstAFI CTTAAG 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 1340
BstAUI TGTACA 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 1404
BstDEI CTNAG 4 cut(s) 500, 1142, 1294, 1371
BstDSI CCRYGG 2 cut(s) 477, 1262
BstEII GGTNACC 1 cut(s) 1178
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 6 cut(s) 820, 835, 926, 1002, 1224, 1396
BstMBI GATC 6 cut(s) 817, 832, 923, 999, 1221, 1393
BstMCI CGRYCG 1 cut(s) 1262
BstMWI GCNNNNNNNGC 2 cut(s) 273, 1340
BstNI CCWGG 4 cut(s) 123, 270, 915, 1214
BstNSI RCATGY 2 cut(s) 866, 1192
BstPAI GACNNNNGTC 1 cut(s) 1320
BstPI GGTNACC 1 cut(s) 1178
BstSCI CCNGG 4 cut(s) 121, 268, 913, 1212
BstSFI CTRYAG 1 cut(s) 1318
BstV1I GCAGC 4 cut(s) 260, 752, 825, 836
BstV2I GAAGAC 2 cut(s) 243, 677
BstX2I RGATCY 2 cut(s) 817, 1221
BstXI CCANNNNNNTGG 1 cut(s) 375
BstYI RGATCY 2 cut(s) 817, 1221
BstZI CGGCCG 1 cut(s) 1259
Bsu15I ATCGAT 1 cut(s) 462
BsuI GTATCC 1 cut(s) 1283
BsuRI GGCC 4 cut(s) 267, 387, 890, 1261
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 2 cut(s) 477, 1262
BtgZI GCGATG 1 cut(s) 1400
BtsCI GGATG 1 cut(s) 520
BtsIMutI CAGTG 4 cut(s) 258, 1150, 1180, 1252
BveI ACCTGC 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 1404
Cfr10I RCCGGY 1 cut(s) 981
Cfr13I GGNCC 5 cut(s) 136, 266, 308, 385, 481
ClaI ATCGAT 1 cut(s) 462
CsiI ACCWGGT 1 cut(s) 121
Csp6I GTAC 4 cut(s) 56, 593, 985, 1363
CviAII CATG 5 cut(s) 375, 863, 1184, 1189, 1349
CviQI GTAC 4 cut(s) 56, 593, 985, 1363
DdeI CTNAG 4 cut(s) 500, 1142, 1294, 1371
DpnI GATC 6 cut(s) 819, 834, 925, 1001, 1223, 1395
DpnII GATC 6 cut(s) 817, 832, 923, 999, 1221, 1393
DrdI GACNNNNNNGTC 1 cut(s) 1028
DseDI GACNNNNNNGTC 1 cut(s) 1028
EaeI YGGCCR 1 cut(s) 1259
EagI CGGCCG 1 cut(s) 1259
Eam1104I CTCTTC 2 cut(s) 585, 1070
EarI CTCTTC 2 cut(s) 585, 1070
Ecl136II GAGCTC 1 cut(s) 1163
EclXI CGGCCG 1 cut(s) 1259
Eco24I GRGCYC 2 cut(s) 633, 1165
Eco47I GGWCC 3 cut(s) 136, 308, 481
Eco52I CGGCCG 1 cut(s) 1259
Eco53kI GAGCTC 1 cut(s) 1163
Eco88I CYCGRG 1 cut(s) 1358
Eco91I GGTNACC 1 cut(s) 1178
EcoICRI GAGCTC 1 cut(s) 1163
EcoO109I RGGNCCY 3 cut(s) 136, 266, 385
EcoO65I GGTNACC 1 cut(s) 1178
EcoRI GAATTC 1 cut(s) 573
EcoRII CCWGG 4 cut(s) 121, 268, 913, 1212
EcoT22I ATGCAT 1 cut(s) 864
EcoT38I GRGCYC 2 cut(s) 633, 1165
FaeI CATG 5 cut(s) 378, 866, 1187, 1192, 1352
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 1 cut(s) 149
FatI CATG 5 cut(s) 374, 862, 1183, 1188, 1348
FauI CCCGC 1 cut(s) 1336
FauNDI CATATG 1 cut(s) 325
FbaI TGATCA 1 cut(s) 832
FblI GTMKAC 2 cut(s) 346, 752
Fnu4HI GCNGC 6 cut(s) 274, 766, 769, 825, 839, 1259
FokI GGATG 1 cut(s) 507
FriOI GRGCYC 2 cut(s) 633, 1165
Fsp4HI GCNGC 6 cut(s) 274, 766, 769, 825, 839, 1259
GluI GCNGC 6 cut(s) 274, 766, 769, 825, 839, 1259
GsuI CTGGAG 2 cut(s) 282, 642
HaeIII GGCC 4 cut(s) 267, 387, 890, 1261
HapII CCGG 2 cut(s) 423, 982
Hin1II CATG 5 cut(s) 378, 866, 1187, 1192, 1352
HincII GTYRAC 3 cut(s) 694, 703, 723
HindII GTYRAC 3 cut(s) 694, 703, 723
HinfI GANTC 9 cut(s) 179, 215, 364, 407, 426, 560, 749, 959, 1138
HpaII CCGG 2 cut(s) 423, 982
HphI GGTGA 5 cut(s) 103, 188, 402, 785, 1190
Hpy188I TCNGA 3 cut(s) 736, 932, 1297
Hpy188III TCNNGA 2 cut(s) 570, 1358
HpyAV CCTTC 6 cut(s) 125, 154, 301, 443, 653, 1166
HpyCH4III ACNGT 6 cut(s) 805, 881, 1111, 1175, 1247, 1322
HpyCH4IV ACGT 2 cut(s) 780, 1047
HpyCH4V TGCA 6 cut(s) 713, 824, 862, 971, 1327, 1334
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 1340
HpyF3I CTNAG 4 cut(s) 500, 1142, 1294, 1371
HpySE526I ACGT 2 cut(s) 780, 1047
Hsp92II CATG 5 cut(s) 378, 866, 1187, 1192, 1352
Ksp22I TGATCA 1 cut(s) 832
Kzo9I GATC 6 cut(s) 817, 832, 923, 999, 1221, 1393
LmnI GCTCC 3 cut(s) 21, 628, 1360
Lsp1109I GCAGC 4 cut(s) 260, 752, 825, 836
LweI GCATC 1 cut(s) 463
MabI ACCWGGT 1 cut(s) 121
MaeII ACGT 2 cut(s) 780, 1047
MaeIII GTNAC 9 cut(s) 109, 176, 452, 611, 773, 811, 1111, 1145, 1178
MalI GATC 6 cut(s) 819, 834, 925, 1001, 1223, 1395
MboI GATC 6 cut(s) 817, 832, 923, 999, 1221, 1393
MboII GAAGA 9 cut(s) 248, 482, 572, 682, 836, 920, 930, 933, 1087
MfeI CAATTG 1 cut(s) 194
MflI RGATCY 2 cut(s) 817, 1221
MhlI GDGCHC 2 cut(s) 633, 1165
MluCI AATT 5 cut(s) 194, 527, 573, 849, 1234
MlyI GAGTC 3 cut(s) 173, 224, 758
MnlI CCTC 9 cut(s) 106, 199, 252, 257, 361, 534, 588, 641, 1071
Mph1103I ATGCAT 1 cut(s) 864
MseI TTAA 5 cut(s) 27, 390, 783, 852, 1044
MslI CAYNNNNRTG 2 cut(s) 373, 976
MspA1I CMGCKG 1 cut(s) 838
MspCI CTTAAG 1 cut(s) 26
MspI CCGG 2 cut(s) 423, 982
MspR9I CCNGG 4 cut(s) 123, 270, 915, 1214
MunI CAATTG 1 cut(s) 194
MvaI CCWGG 4 cut(s) 123, 270, 915, 1214
MwoI GCNNNNNNNGC 2 cut(s) 273, 1340
NdeI CATATG 1 cut(s) 325
NdeII GATC 6 cut(s) 817, 832, 923, 999, 1221, 1393
NlaIII CATG 5 cut(s) 378, 866, 1187, 1192, 1352
NmuCI GTSAC 7 cut(s) 109, 176, 773, 811, 1111, 1145, 1178
NsiI ATGCAT 1 cut(s) 864
NspI RCATGY 2 cut(s) 866, 1192
PciI ACATGT 1 cut(s) 1188
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PfeI GAWTC 6 cut(s) 364, 407, 426, 560, 959, 1138
PkrI GCNGC 6 cut(s) 275, 767, 770, 826, 840, 1260
PleI GAGTC 3 cut(s) 173, 223, 757
PpsI GAGTC 3 cut(s) 173, 223, 757
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 1188
PshAI GACNNNNGTC 1 cut(s) 1320
PshBI ATTAAT 1 cut(s) 852
Psp124BI GAGCTC 1 cut(s) 1165
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 4 cut(s) 121, 268, 913, 1212
PspEI GGTNACC 1 cut(s) 1178
PspGI CCWGG 4 cut(s) 121, 268, 913, 1212
PspPI GGNCC 5 cut(s) 136, 266, 308, 385, 481
PspPPI RGGWCCY 1 cut(s) 136
PsrI GAACNNNNNNTAC 2 cut(s) 48, 80
PsuI RGATCY 2 cut(s) 817, 1221
PvuII CAGCTG 1 cut(s) 838
RsaI GTAC 4 cut(s) 57, 594, 986, 1364
RsaNI GTAC 4 cut(s) 56, 593, 985, 1363
RseI CAYNNNNRTG 2 cut(s) 373, 976
SacI GAGCTC 1 cut(s) 1165
SaqAI TTAA 5 cut(s) 27, 390, 783, 852, 1044
SatI GCNGC 6 cut(s) 274, 766, 769, 825, 839, 1259
Sau3AI GATC 6 cut(s) 817, 832, 923, 999, 1221, 1393
Sau96I GGNCC 5 cut(s) 136, 266, 308, 385, 481
SchI GAGTC 3 cut(s) 173, 224, 758
ScrFI CCNGG 4 cut(s) 123, 270, 915, 1214
SduI GDGCHC 2 cut(s) 633, 1165
SexAI ACCWGGT 1 cut(s) 121
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 1 cut(s) 1318
SinI GGWCC 3 cut(s) 136, 308, 481
SmiMI CAYNNNNRTG 2 cut(s) 373, 976
SmlI CTYRAG 2 cut(s) 26, 73
SmoI CTYRAG 2 cut(s) 26, 73
Sse9I AATT 5 cut(s) 194, 527, 573, 849, 1234
SsiI CCGC 5 cut(s) 768, 771, 1033, 1258, 1343
SspI AATATT 1 cut(s) 856
SstI GAGCTC 1 cut(s) 1165
StyD4I CCNGG 4 cut(s) 121, 268, 913, 1212
TaaI ACNGT 6 cut(s) 805, 881, 1111, 1175, 1247, 1322
TaiI ACGT 2 cut(s) 783, 1050
TaqI TCGA 4 cut(s) 153, 462, 646, 1159
TasI AATT 5 cut(s) 194, 527, 573, 849, 1234
TatI WGTACW 3 cut(s) 55, 592, 1362
TauI GCSGC 2 cut(s) 771, 1261
TfiI GAWTC 6 cut(s) 364, 407, 426, 560, 959, 1138
Tru1I TTAA 5 cut(s) 27, 390, 783, 852, 1044
Tru9I TTAA 5 cut(s) 27, 390, 783, 852, 1044
TscAI CASTG 4 cut(s) 265, 1150, 1180, 1252
TseFI GTSAC 7 cut(s) 109, 176, 773, 811, 1111, 1145, 1178
TseI GCWGC 4 cut(s) 273, 765, 824, 838
Tsp45I GTSAC 7 cut(s) 109, 176, 773, 811, 1111, 1145, 1178
TspDTI ATGAA 5 cut(s) 315, 347, 552, 673, 753
TspGWI ACGGA 1 cut(s) 1214
TspRI CASTG 4 cut(s) 265, 1150, 1180, 1252
Vha464I CTTAAG 1 cut(s) 26
VpaK11BI GGWCC 3 cut(s) 136, 308, 481
VspI ATTAAT 1 cut(s) 852
XapI RAATTY 2 cut(s) 573, 1234
XceI RCATGY 2 cut(s) 866, 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 2 cut(s) 346, 752
Zsp2I ATGCAT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.