Rw4G007060

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000839
Physical Location & Seq
Forward (+)
14768559 .. 14773640
5082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007060.1

Sequence Viewer

Length: 1434 bp
ATGCCCACCATGACGAAATTGATGGGTATGCGACTACTTCTTGCATTTGTTTTCACTCTCATACTTGCTTCAACTTCTTCGGCCAATGTCACTACCACCACAATTACCACACTACCTGGTTACTCAGGCGACCTCCCTTTCTCCTTCGAAACTGGGTACGTTGGTGTAGGTGACAATGAAGAAGTGCAACTATTTTACTACTTTGTCGAGTCTCAGAGGACTCCAGCACAGGATCCTCTGCTGTTGTGGATCCCTGCGGGACCTGGTTGCTCTGGTCAGATTGTACTCTTCTTTGAGAGTGGTCCACTACTTTTCGATTATGGTGAATACGATGGGAGCCTACCAACACTTCACGACAACCCTTTCACATGGACAGAGAGCCTCAACCTCATATACGTGGACGCACCTGTTGGTGCTGGATACTCCTACTCAACAACCCAAAGTGGTTATGTAATGGACGACTATAAACATGTGGCACAACTATATGAATTTCTGCAGAAGTGGTTAGATGCACACCCTACGTACCTGAAAAACATACTCTACATTGGTGGTGACTCCTACTCTGGAATGCCTGTTCCCATGCTTGCACAAACAATAGTCAATGGTAATGAAAATGGAAGTATCCCCATGATGAATTTCAGAGGATATGTGATTGGGAACCCAGTAACTGATACAACAGTTGATGGTAATGCACAAATTCCTATTGCTCACCGGCTGTCTCTTATATCGGATCAACTATATAAGTCACTTAAAACAAGTTGCCACGGCGAATATGTAACTGTAAATTCCAGCAATCATGCATGCATTGCAGATCTTGATGCCTTTGATGCACTAACGAGTGACATAAATACATTACATGTCTTGGAACCATTATGTAGTAATGGTTTACCTACACAAAAGGAAATGGTTTTGACCCGGAGATATCTCAAGGAAACATCTAGCGGTTTACAAAGATCACGACCTAACGGCCCTGCACTTTGGTGTCGGAGTTATCGTAGCATGCTCAACACCGTTTGGGCTAACAACAAAGGTGTTCAAGATGCTCTTGGTGTCCGAAGGGGCACGAAAAGGCTTTGGCAACACTGTAATACGACATTGGACTACACTGAAGATGTCACCAGCGTAATCATTTATCACCAAAGTCTTTCAAAAATGGCTGATCTACGTGTTCTGATATACAGTGGTGATCATGACATGCAAGTTCCTCATATTGGTACACAAAGATGGATAAGCACACTGAATTTGACTGAGGATGAATCCTGGAGGACATGGTCAGTTGATGGCCAAACTGCAGGATACACGAAGAAGTTGATCAATGAATATATCACTTTGACATATGCGACTGTAAAGGCAAGAGGCCATATTGCTGCAGAGACAAAGGTCAAGGAATGCGCTGCCATGATTAATAGATGGATGGCTTACTTCCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

477

Amino Acids

53.29

Weight (kDa)

5.54

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 39 - 472 3.9e-108 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 257, 942
AclWI GGATC 5 cut(s) 227, 240, 244, 257, 738
AcoI YGGCCR 2 cut(s) 81, 1282
AcsI RAATTY 5 cut(s) 488, 634, 696, 784, 1240
AcuI CTGAAG 1 cut(s) 1128
AfaI GTAC 4 cut(s) 158, 285, 524, 1216
AfiI CCNNNNNNNGG 1 cut(s) 1211
AflIII ACRYGT 3 cut(s) 469, 856, 1165
AgsI TTSAA 3 cut(s) 72, 1037, 1149
AjnI CCWGG 3 cut(s) 115, 262, 1259
AleI CACNNNNGTG 1 cut(s) 979
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
Alw26I GTCTC 3 cut(s) 216, 723, 1367
AlwI GGATC 5 cut(s) 227, 240, 244, 257, 738
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 4 cut(s) 81, 967, 1282, 1357
ApeKI GCWGC 2 cut(s) 1367, 1394
ApoI RAATTY 5 cut(s) 488, 634, 696, 784, 1240
AseI ATTAAT 1 cut(s) 1404
AspLEI GCGC 1 cut(s) 1394
AspS9I GGNCC 3 cut(s) 260, 302, 968
AsuC2I CCSGG 1 cut(s) 916
AsuHPI GGTGA 7 cut(s) 182, 335, 563, 701, 1108, 1127, 1196
AsuII TTCGAA 1 cut(s) 147
AvaII GGWCC 2 cut(s) 260, 302
BaeGI GKGCMC 1 cut(s) 1064
BaeI ACNNNNGTAYC 4 cut(s) 148, 181, 663, 696
BalI TGGCCA 1 cut(s) 1284
BamHI GGATCC 2 cut(s) 232, 249
BbvI GCAGC 2 cut(s) 1354, 1381
BccI CCATC 7 cut(s) 16, 326, 677, 1218, 1274, 1404, 1408
BceAI ACGGC 2 cut(s) 781, 982
BciT130I CCWGG 3 cut(s) 117, 264, 1261
BciVI GTATCC 3 cut(s) 413, 632, 1289
BclI TGATCA 2 cut(s) 1186, 1311
BcnI CCSGG 1 cut(s) 916
BcoDI GTCTC 3 cut(s) 216, 723, 1367
BfaI CTAG 1 cut(s) 939
BfmI CTRYAG 3 cut(s) 494, 1290, 1368
BfuI GTATCC 3 cut(s) 413, 632, 1289
BglII AGATCT 1 cut(s) 811
BisI GCNGC 2 cut(s) 1368, 1395
BlsI GCNGC 2 cut(s) 1369, 1396
Bme1390I CCNGG 4 cut(s) 117, 264, 916, 1261
Bme18I GGWCC 2 cut(s) 260, 302
BmgT120I GGNCC 3 cut(s) 260, 302, 968
BmiI GGNNCC 6 cut(s) 234, 251, 261, 338, 659, 867
BmrFI CCNGG 4 cut(s) 117, 264, 916, 1261
BmrI ACTGGG 2 cut(s) 162, 656
BmsI GCATC 4 cut(s) 499, 808, 817, 1030
BmuI ACTGGG 2 cut(s) 162, 656
BoxI GACNNNNGTC 1 cut(s) 1379
BpmI CTGGAG 2 cut(s) 207, 1282
Bpu14I TTCGAA 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 911
BpuMI CCSGG 1 cut(s) 916
BsaAI YACGTR 3 cut(s) 397, 522, 1166
BsaJI CCNNGG 1 cut(s) 763
BsaXI ACNNNNNCTCC 2 cut(s) 979, 1009
Bsc4I CCNNNNNNNGG 1 cut(s) 1211
Bse118I RCCGGY 1 cut(s) 711
Bse1I ACTGG 2 cut(s) 157, 662
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 3 cut(s) 117, 264, 1261
BseDI CCNNGG 1 cut(s) 763
BseGI GGATG 2 cut(s) 1258, 1419
BseLI CCNNNNNNNGG 1 cut(s) 1211
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 3 cut(s) 138, 227, 1239
BseNI ACTGG 2 cut(s) 157, 662
BseSI GKGCMC 1 cut(s) 1064
BseXI GCAGC 2 cut(s) 1354, 1381
BsgI GTGCAG 1 cut(s) 957
BshFI GGCC 4 cut(s) 83, 969, 1284, 1359
BsiSI CCGG 2 cut(s) 712, 916
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 1 cut(s) 1211
BsmAI GTCTC 3 cut(s) 216, 723, 1367
BsmFI GGGAC 1 cut(s) 273
BsmI GAATGC 2 cut(s) 573, 1394
BsnI GGCC 4 cut(s) 83, 969, 1284, 1359
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 1064
Bsp143I GATC 8 cut(s) 232, 249, 730, 811, 953, 1159, 1186, 1311
BspACI CCGC 2 cut(s) 257, 942
BspANI GGCC 4 cut(s) 83, 969, 1284, 1359
BspCNI CTCAG 3 cut(s) 137, 226, 1240
BspHI TCATGA 1 cut(s) 1189
BspLI GGNNCC 6 cut(s) 234, 251, 261, 338, 659, 867
BspMAI CTGCAG 3 cut(s) 498, 1294, 1372
BspPI GGATC 5 cut(s) 227, 240, 244, 257, 738
BspT104I TTCGAA 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 1 cut(s) 711
BsrI ACTGG 2 cut(s) 157, 662
BssAI RCCGGY 1 cut(s) 711
BssECI CCNNGG 1 cut(s) 763
BssMI GATC 8 cut(s) 232, 249, 730, 811, 953, 1159, 1186, 1311
Bst2UI CCWGG 3 cut(s) 117, 264, 1261
Bst4CI ACNGT 6 cut(s) 679, 781, 1012, 1085, 1181, 1345
Bst6I CTCTTC 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 806
BstBAI YACGTR 3 cut(s) 397, 522, 1166
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 3 cut(s) 585, 802, 1001
BstDEI CTNAG 3 cut(s) 124, 213, 1248
BstDSI CCRYGG 1 cut(s) 763
BstF5I GGATG 2 cut(s) 1258, 1419
BstHHI GCGC 1 cut(s) 1394
BstKTI GATC 8 cut(s) 235, 252, 733, 814, 956, 1162, 1189, 1314
BstMAI GTCTC 3 cut(s) 216, 723, 1367
BstMBI GATC 8 cut(s) 232, 249, 730, 811, 953, 1159, 1186, 1311
BstMWI GCNNNNNNNGC 2 cut(s) 806, 827
BstNI CCWGG 3 cut(s) 117, 264, 1261
BstNSI RCATGY 5 cut(s) 473, 804, 860, 1003, 1198
BstPAI GACNNNNGTC 1 cut(s) 1379
BstSCI CCNGG 4 cut(s) 115, 262, 914, 1259
BstSFI CTRYAG 3 cut(s) 494, 1290, 1368
BstSLI GKGCMC 1 cut(s) 1064
BstSNI TACGTA 1 cut(s) 522
BstV1I GCAGC 2 cut(s) 1354, 1381
BstX2I RGATCY 3 cut(s) 232, 249, 811
BstYI RGATCY 3 cut(s) 232, 249, 811
BsuI GTATCC 3 cut(s) 413, 632, 1289
BsuRI GGCC 4 cut(s) 83, 969, 1284, 1359
BtgI CCRYGG 1 cut(s) 763
BtsCI GGATG 2 cut(s) 1258, 1419
BtsIMutI CAGTG 4 cut(s) 1081, 1104, 1186, 1235
Cac8I GCNNGC 3 cut(s) 585, 802, 1001
CaiI CAGNNNCTG 1 cut(s) 668
CciI TCATGA 1 cut(s) 1189
CfoI GCGC 1 cut(s) 1394
Cfr10I RCCGGY 1 cut(s) 711
Cfr13I GGNCC 3 cut(s) 260, 302, 968
CseI GACGC 1 cut(s) 410
CsiI ACCWGGT 2 cut(s) 115, 262
Csp6I GTAC 4 cut(s) 157, 284, 523, 1215
CviQI GTAC 4 cut(s) 157, 284, 523, 1215
DdeI CTNAG 3 cut(s) 124, 213, 1248
DpnI GATC 8 cut(s) 234, 251, 732, 813, 955, 1161, 1188, 1313
DpnII GATC 8 cut(s) 232, 249, 730, 811, 953, 1159, 1186, 1311
EaeI YGGCCR 2 cut(s) 81, 1282
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco105I TACGTA 1 cut(s) 522
Eco32I GATATC 1 cut(s) 923
Eco47I GGWCC 2 cut(s) 260, 302
Eco57I CTGAAG 1 cut(s) 1128
EcoO109I RGGNCCY 1 cut(s) 260
EcoRII CCWGG 3 cut(s) 115, 262, 1259
EcoRV GATATC 1 cut(s) 923
EcoT22I ATGCAT 2 cut(s) 802, 806
FalI AAGNNNNNCTT 2 cut(s) 1029, 1061
FaqI GGGAC 1 cut(s) 273
FauI CCCGC 1 cut(s) 250
FauNDI CATATG 1 cut(s) 1336
FbaI TGATCA 2 cut(s) 1186, 1311
Fnu4HI GCNGC 2 cut(s) 1368, 1395
FokI GGATG 2 cut(s) 1265, 1426
Fsp4HI GCNGC 2 cut(s) 1368, 1395
FspBI CTAG 1 cut(s) 939
GlaI GCGC 1 cut(s) 1393
GluI GCNGC 2 cut(s) 1368, 1395
GsuI CTGGAG 2 cut(s) 207, 1282
HaeIII GGCC 4 cut(s) 83, 969, 1284, 1359
HapII CCGG 2 cut(s) 712, 916
HgaI GACGC 1 cut(s) 410
HhaI GCGC 1 cut(s) 1394
Hin6I GCGC 1 cut(s) 1392
HinP1I GCGC 1 cut(s) 1392
HinfI GANTC 4 cut(s) 209, 220, 554, 1256
HpaII CCGG 2 cut(s) 712, 916
HphI GGTGA 7 cut(s) 182, 335, 563, 701, 1108, 1127, 1196
Hpy166II GTNNAC 5 cut(s) 305, 400, 887, 947, 1217
Hpy188I TCNGA 7 cut(s) 216, 279, 641, 730, 987, 1055, 1173
Hpy188III TCNNGA 6 cut(s) 353, 564, 815, 957, 1037, 1190
Hpy8I GTNNAC 5 cut(s) 305, 400, 887, 947, 1217
HpyAV CCTTC 2 cut(s) 154, 1050
HpyCH4III ACNGT 6 cut(s) 679, 781, 1012, 1085, 1181, 1345
HpyCH4IV ACGT 4 cut(s) 159, 396, 521, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 806, 827
HpyF3I CTNAG 3 cut(s) 124, 213, 1248
HpySE526I ACGT 4 cut(s) 159, 396, 521, 1165
HspAI GCGC 1 cut(s) 1392
Ksp22I TGATCA 2 cut(s) 1186, 1311
Kzo9I GATC 8 cut(s) 232, 249, 730, 811, 953, 1159, 1186, 1311
LmnI GCTCC 1 cut(s) 336
Lsp1109I GCAGC 2 cut(s) 1354, 1381
LweI GCATC 4 cut(s) 499, 808, 817, 1030
MabI ACCWGGT 2 cut(s) 115, 262
MaeI CTAG 1 cut(s) 939
MaeII ACGT 4 cut(s) 159, 396, 521, 1165
MaeIII GTNAC 9 cut(s) 88, 119, 170, 551, 664, 744, 775, 839, 1114
MalI GATC 8 cut(s) 234, 251, 732, 813, 955, 1161, 1188, 1313
MboI GATC 8 cut(s) 232, 249, 730, 811, 953, 1159, 1186, 1311
MboII GAAGA 5 cut(s) 69, 191, 280, 1121, 1315
MflI RGATCY 3 cut(s) 232, 249, 811
MhlI GDGCHC 1 cut(s) 1064
MlsI TGGCCA 1 cut(s) 1284
MluCI AATT 7 cut(s) 17, 102, 488, 634, 696, 784, 1240
MluNI TGGCCA 1 cut(s) 1284
MlyI GAGTC 3 cut(s) 214, 218, 548
MmeI TCCRAC 1 cut(s) 965
Mox20I TGGCCA 1 cut(s) 1284
Mph1103I ATGCAT 2 cut(s) 802, 806
MscI TGGCCA 1 cut(s) 1284
MseI TTAA 2 cut(s) 750, 1404
MslI CAYNNNNRTG 3 cut(s) 395, 979, 1222
Msp20I TGGCCA 1 cut(s) 1284
MspI CCGG 2 cut(s) 712, 916
MspR9I CCNGG 4 cut(s) 117, 264, 916, 1261
Mva1269I GAATGC 2 cut(s) 573, 1394
MvaI CCWGG 3 cut(s) 117, 264, 1261
MwoI GCNNNNNNNGC 2 cut(s) 806, 827
NciI CCSGG 1 cut(s) 916
NdeI CATATG 1 cut(s) 1336
NdeII GATC 8 cut(s) 232, 249, 730, 811, 953, 1159, 1186, 1311
NlaIV GGNNCC 6 cut(s) 234, 251, 261, 338, 659, 867
NmuCI GTSAC 6 cut(s) 88, 170, 551, 744, 839, 1114
NsiI ATGCAT 2 cut(s) 802, 806
NspI RCATGY 5 cut(s) 473, 804, 860, 1003, 1198
NspV TTCGAA 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 979
PaeI GCATGC 2 cut(s) 804, 1003
PagI TCATGA 1 cut(s) 1189
PciI ACATGT 2 cut(s) 469, 856
PcsI WCGNNNNNNNCGW 1 cut(s) 991
PctI GAATGC 2 cut(s) 573, 1394
PfeI GAWTC 1 cut(s) 1256
PflFI GACNNNGTC 1 cut(s) 1270
PfoI TCCNGGA 1 cut(s) 1259
PkrI GCNGC 2 cut(s) 1369, 1396
PleI GAGTC 3 cut(s) 214, 217, 548
PpsI GAGTC 3 cut(s) 214, 217, 548
Ppu21I YACGTR 3 cut(s) 397, 522, 1166
PpuMI RGGWCCY 1 cut(s) 260
PscI ACATGT 2 cut(s) 469, 856
PshAI GACNNNNGTC 1 cut(s) 1379
PshBI ATTAAT 1 cut(s) 1404
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 3 cut(s) 115, 262, 1259
PspGI CCWGG 3 cut(s) 115, 262, 1259
PspN4I GGNNCC 6 cut(s) 234, 251, 261, 338, 659, 867
PspPI GGNCC 3 cut(s) 260, 302, 968
PspPPI RGGWCCY 1 cut(s) 260
PstI CTGCAG 3 cut(s) 498, 1294, 1372
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 3 cut(s) 232, 249, 811
PsyI GACNNNGTC 1 cut(s) 1270
RsaI GTAC 4 cut(s) 158, 285, 524, 1216
RsaNI GTAC 4 cut(s) 157, 284, 523, 1215
RseI CAYNNNNRTG 3 cut(s) 395, 979, 1222
SaqAI TTAA 2 cut(s) 750, 1404
SatI GCNGC 2 cut(s) 1368, 1395
Sau3AI GATC 8 cut(s) 232, 249, 730, 811, 953, 1159, 1186, 1311
Sau96I GGNCC 3 cut(s) 260, 302, 968
SchI GAGTC 3 cut(s) 214, 218, 548
ScrFI CCNGG 4 cut(s) 117, 264, 916, 1261
SduI GDGCHC 1 cut(s) 1064
SexAI ACCWGGT 2 cut(s) 115, 262
SfaNI GCATC 4 cut(s) 499, 808, 817, 1030
SfcI CTRYAG 3 cut(s) 494, 1290, 1368
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 2 cut(s) 260, 302
SmiMI CAYNNNNRTG 3 cut(s) 395, 979, 1222
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
SnaBI TACGTA 1 cut(s) 522
SphI GCATGC 2 cut(s) 804, 1003
Sse9I AATT 7 cut(s) 17, 102, 488, 634, 696, 784, 1240
SsiI CCGC 2 cut(s) 257, 942
SspMI CTAG 1 cut(s) 939
StyD4I CCNGG 4 cut(s) 115, 262, 914, 1259
TaaI ACNGT 6 cut(s) 679, 781, 1012, 1085, 1181, 1345
TaiI ACGT 4 cut(s) 162, 399, 524, 1168
TaqI TCGA 3 cut(s) 147, 207, 315
TasI AATT 7 cut(s) 17, 102, 488, 634, 696, 784, 1240
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 1 cut(s) 1256
Tru1I TTAA 2 cut(s) 750, 1404
Tru9I TTAA 2 cut(s) 750, 1404
TscAI CASTG 4 cut(s) 1088, 1111, 1186, 1242
TseFI GTSAC 6 cut(s) 88, 170, 551, 744, 839, 1114
TseI GCWGC 2 cut(s) 1367, 1394
Tsp45I GTSAC 6 cut(s) 88, 170, 551, 744, 839, 1114
TspDTI ATGAA 6 cut(s) 192, 501, 624, 647, 1269, 1332
TspRI CASTG 4 cut(s) 1088, 1111, 1186, 1242
Tth111I GACNNNGTC 1 cut(s) 1270
VpaK11BI GGWCC 2 cut(s) 260, 302
VspI ATTAAT 1 cut(s) 1404
XapI RAATTY 5 cut(s) 488, 634, 696, 784, 1240
XceI RCATGY 5 cut(s) 473, 804, 860, 1003, 1198
XspI CTAG 1 cut(s) 939
Zsp2I ATGCAT 2 cut(s) 802, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.