Rh4AG108100

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000839
Physical Location & Seq
Forward (+)
23502065 .. 23506037
3973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG108100.1

Sequence Viewer

Length: 1428 bp
ATGGCACCAGAAAACCTTCAATGTCTTAAGAAATATATCGGTCCATTATTGTGTTTGCATGTAGTTCTTTTGTCAAGTTTCTGTGGGATTGCTACCTCCCAAACCATAGTCACCAGTTTACCTGGTTTTGAAGGGGACCTGCCATTCACACTTGAAACTGGGTATGTGGGTGTGGGTGACTCAAACTCCTCCCAATTGTTCTACTATTTTGTTGAGTCGACGAGAAGCCCTTCCGAAGACCCTCTTATGCTTTGGTTCACTGGAGGCCCTGGCTGCTCTGTTCTCTCTGCCTTCTTTTATGAAAGTGGTCCACTATCTTTCACATATGTAGATTACAATGGAAGTCTACCCTCACTTCATGTCAACCCATATGCATGGACACAGGGCCTCAACATAATATATGTAGATTCACCTGTTGGCACTGGATTCTCTTACTCTACAACCCAAGAAGGTTACTATATAGATGATGAAGATGCCGTGGACCAGAGCGTTGAGTTTCTGCGGAAGTGGCTGTTGGCTCATCCTCAATATTTGAACAATATACTCTACATTGGTGGCGATTCTTATTCAGGAATTCCCCTTCCTATAATAGTACAAAAACTTTTTGATGGTAACAATGCTGGAGTGGAGCCCTATCTAAACCTTCGAGGTTATATACTTGGAAACCCGAAGACTGATGACTTCATTGATGTCAACTCAGTGAACCCATTTGCACACAGGTTGACACTCATATCAGATGAACTTTATGAGGCTACCAAAGAAAGCTGCGGTGGTGATTACGTTAATGTAGACGATAACAACACAGCATGTGTGATAGATCTTGCAACTATTGATCAGCTGCTTCTTCAAATTAATATTATGCATGTCTTGGAACCCAATTGTCAAGTGGCCTTACCAAACAACAGAGAAGAAGCAGGAAGAAGATCAATCCGAGAACTACCTGAGAATTTTCTAACAGAATCTACTCCTGCATTGTGGTGCCGGTACTACAACTACGTGATCTTGGGTGTGTGGGCTAATGACCACAGTGTCCGCAAAGCTCTTAATGTCAGCGAGAACTCCATTGGTGTCTGGAAGAGGTGTAATATGAGCATAGCTTACACAAAGACCGTCACAAGTAGCGTTGAGTATCATAAGAATCTCAGTAACACAAACCTTCGAGCTCTTATATACAGTGGTGACCATGACATGTCTGTTCCACACATCGGCACCCAGGAGTGGATCCGTGATCTAAATTTGACAAATAGTGAGATTTGGCGGCCGTGGTATGTAGATGGTCAAGTTGCTGGATACTCAGAAAAGTTCTCAAACGTTGACTACAATCTGCAATATGCAACTGTAAAGGGTGCCGGTCATGTGGCTCCAGAGTACAAAGCAAAGGAATGTTATGCGATGATTGATCGGTGGCTCGCTTATTACCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

475

Amino Acids

53.32

Weight (kDa)

4.69

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 41 - 471 3.1e-118 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1028
Acc36I ACCTGC 1 cut(s) 147
AccB1I GGYRCC 4 cut(s) 4, 978, 1208, 1346
AccI GTMKAC 3 cut(s) 218, 346, 789
AciI CCGC 4 cut(s) 502, 768, 1033, 1258
AclI AACGTT 1 cut(s) 1311
AclWI GGATC 2 cut(s) 1216, 1229
AcoI YGGCCR 1 cut(s) 1259
AcsI RAATTY 3 cut(s) 573, 946, 1234
AfaI GTAC 3 cut(s) 594, 986, 1370
AfiI CCNNNNNNNGG 2 cut(s) 1205, 1218
AflII CTTAAG 1 cut(s) 26
AflIII ACRYGT 1 cut(s) 1188
AgsI TTSAA 5 cut(s) 20, 131, 155, 535, 848
AjnI CCWGG 3 cut(s) 121, 268, 1212
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 5 cut(s) 765, 838, 1040, 1097, 1163
AluI AGCT 5 cut(s) 765, 838, 1040, 1097, 1163
Alw21I GWGCWC 1 cut(s) 1165
AlwI GGATC 2 cut(s) 1216, 1229
AoxI GGCC 4 cut(s) 265, 385, 888, 1259
ApeKI GCWGC 3 cut(s) 273, 765, 838
ApoI RAATTY 3 cut(s) 573, 946, 1234
AseI ATTAAT 1 cut(s) 852
Asp700I GAANNNNTTC 2 cut(s) 15, 229
AspS9I GGNCC 6 cut(s) 41, 136, 266, 308, 385, 481
AsuHPI GGTGA 5 cut(s) 103, 188, 402, 785, 1190
AvaII GGWCC 4 cut(s) 41, 136, 308, 481
BamHI GGATCC 1 cut(s) 1221
BanI GGYRCC 4 cut(s) 4, 978, 1208, 1346
BanII GRGCYC 2 cut(s) 633, 1165
BbsI GAAGAC 2 cut(s) 243, 677
Bbv12I GWGCWC 1 cut(s) 1165
BbvI GCAGC 3 cut(s) 260, 752, 825
BccI CCATC 2 cut(s) 602, 1268
BceAI ACGGC 2 cut(s) 461, 1246
BciT130I CCWGG 3 cut(s) 123, 270, 1214
BciVI GTATCC 1 cut(s) 1283
BclI TGATCA 1 cut(s) 832
BfrI CTTAAG 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 147
BfuI GTATCC 1 cut(s) 1283
BglII AGATCT 1 cut(s) 817
BisI GCNGC 4 cut(s) 274, 766, 839, 1259
BlsI GCNGC 4 cut(s) 275, 767, 840, 1260
Bme1390I CCNGG 3 cut(s) 123, 270, 1214
Bme18I GGWCC 4 cut(s) 41, 136, 308, 481
BmgT120I GGNCC 6 cut(s) 41, 136, 266, 308, 385, 481
BmiI GGNNCC 9 cut(s) 6, 137, 630, 873, 980, 1210, 1223, 1348, 1362
BmrFI CCNGG 3 cut(s) 123, 270, 1214
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 168
BpiI GAAGAC 2 cut(s) 243, 677
BpmI CTGGAG 3 cut(s) 282, 642, 1347
BsaAI YACGTR 1 cut(s) 997
BsaJI CCNNGG 4 cut(s) 268, 477, 1212, 1262
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 1205, 1218
Bse118I RCCGGY 2 cut(s) 981, 1349
Bse1I ACTGG 4 cut(s) 114, 163, 265, 427
BseBI CCWGG 3 cut(s) 123, 270, 1214
BseDI CCNNGG 4 cut(s) 268, 477, 1212, 1262
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 2 cut(s) 1205, 1218
BseMII CTCAG 4 cut(s) 711, 933, 1156, 1308
BseNI ACTGG 4 cut(s) 114, 163, 265, 427
BseRI GAGGAG 1 cut(s) 178
BseX3I CGGCCG 1 cut(s) 1259
BseXI GCAGC 3 cut(s) 260, 752, 825
Bsh1285I CGRYCG 1 cut(s) 1262
BshFI GGCC 4 cut(s) 267, 387, 890, 1261
BshNI GGYRCC 4 cut(s) 4, 978, 1208, 1346
BsiEI CGRYCG 1 cut(s) 1262
BsiHKAI GWGCWC 1 cut(s) 1165
BsiSI CCGG 2 cut(s) 982, 1350
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 1205, 1218
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 4 cut(s) 267, 387, 890, 1261
Bsp1286I GDGCHC 2 cut(s) 633, 1165
Bsp143I GATC 7 cut(s) 817, 832, 923, 999, 1221, 1228, 1399
BspACI CCGC 4 cut(s) 502, 768, 1033, 1258
BspANI GGCC 4 cut(s) 267, 387, 890, 1261
BspCNI CTCAG 4 cut(s) 710, 934, 1155, 1307
BspLI GGNNCC 9 cut(s) 6, 137, 630, 873, 980, 1210, 1223, 1348, 1362
BspMI ACCTGC 1 cut(s) 147
BspPI GGATC 2 cut(s) 1216, 1229
BspT107I GGYRCC 4 cut(s) 4, 978, 1208, 1346
BspTI CTTAAG 1 cut(s) 26
BsrFI RCCGGY 2 cut(s) 981, 1349
BsrI ACTGG 4 cut(s) 114, 163, 265, 427
BssAI RCCGGY 2 cut(s) 981, 1349
BssECI CCNNGG 4 cut(s) 268, 477, 1212, 1262
BssMI GATC 7 cut(s) 817, 832, 923, 999, 1221, 1228, 1399
Bst2UI CCWGG 3 cut(s) 123, 270, 1214
Bst4CI ACNGT 4 cut(s) 1028, 1111, 1175, 1339
Bst6I CTCTTC 1 cut(s) 1070
BstAFI CTTAAG 1 cut(s) 26
BstBAI YACGTR 1 cut(s) 997
BstC8I GCNNGC 1 cut(s) 1410
BstDEI CTNAG 4 cut(s) 697, 942, 1142, 1294
BstDSI CCRYGG 2 cut(s) 477, 1262
BstEII GGTNACC 1 cut(s) 1178
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 7 cut(s) 820, 835, 926, 1002, 1224, 1231, 1402
BstMBI GATC 7 cut(s) 817, 832, 923, 999, 1221, 1228, 1399
BstMCI CGRYCG 1 cut(s) 1262
BstMWI GCNNNNNNNGC 2 cut(s) 273, 508
BstNI CCWGG 3 cut(s) 123, 270, 1214
BstNSI RCATGY 4 cut(s) 62, 810, 866, 1192
BstPI GGTNACC 1 cut(s) 1178
BstSCI CCNGG 3 cut(s) 121, 268, 1212
BstV1I GCAGC 3 cut(s) 260, 752, 825
BstV2I GAAGAC 2 cut(s) 243, 677
BstX2I RGATCY 2 cut(s) 817, 1221
BstXI CCANNNNNNTGG 1 cut(s) 375
BstYI RGATCY 2 cut(s) 817, 1221
BstZI CGGCCG 1 cut(s) 1259
BsuI GTATCC 1 cut(s) 1283
BsuRI GGCC 4 cut(s) 267, 387, 890, 1261
BtgI CCRYGG 2 cut(s) 477, 1262
BtgZI GCGATG 1 cut(s) 1406
BtsCI GGATG 1 cut(s) 520
BtsIMutI CAGTG 5 cut(s) 258, 420, 705, 1033, 1180
BveI ACCTGC 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 1410
Cfr10I RCCGGY 2 cut(s) 981, 1349
Cfr13I GGNCC 6 cut(s) 41, 136, 266, 308, 385, 481
CsiI ACCWGGT 1 cut(s) 121
Csp6I GTAC 3 cut(s) 593, 985, 1369
CviAII CATG 8 cut(s) 59, 359, 375, 807, 863, 1184, 1189, 1355
CviQI GTAC 3 cut(s) 593, 985, 1369
DdeI CTNAG 4 cut(s) 697, 942, 1142, 1294
DpnI GATC 7 cut(s) 819, 834, 925, 1001, 1223, 1230, 1401
DpnII GATC 7 cut(s) 817, 832, 923, 999, 1221, 1228, 1399
DrdI GACNNNNNNGTC 1 cut(s) 1028
DseDI GACNNNNNNGTC 1 cut(s) 1028
EaeI YGGCCR 1 cut(s) 1259
EagI CGGCCG 1 cut(s) 1259
Eam1104I CTCTTC 1 cut(s) 1070
EarI CTCTTC 1 cut(s) 1070
Ecl136II GAGCTC 1 cut(s) 1163
EclXI CGGCCG 1 cut(s) 1259
Eco24I GRGCYC 2 cut(s) 633, 1165
Eco47I GGWCC 4 cut(s) 41, 136, 308, 481
Eco52I CGGCCG 1 cut(s) 1259
Eco53kI GAGCTC 1 cut(s) 1163
Eco91I GGTNACC 1 cut(s) 1178
EcoICRI GAGCTC 1 cut(s) 1163
EcoO109I RGGNCCY 3 cut(s) 136, 266, 385
EcoO65I GGTNACC 1 cut(s) 1178
EcoRI GAATTC 1 cut(s) 573
EcoRII CCWGG 3 cut(s) 121, 268, 1212
EcoT22I ATGCAT 2 cut(s) 376, 864
EcoT38I GRGCYC 2 cut(s) 633, 1165
FaeI CATG 8 cut(s) 62, 362, 378, 810, 866, 1187, 1192, 1358
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 1 cut(s) 149
FatI CATG 8 cut(s) 58, 358, 374, 806, 862, 1183, 1188, 1354
FauNDI CATATG 2 cut(s) 325, 370
FbaI TGATCA 1 cut(s) 832
FblI GTMKAC 3 cut(s) 218, 346, 789
Fnu4HI GCNGC 4 cut(s) 274, 766, 839, 1259
FokI GGATG 1 cut(s) 507
FriOI GRGCYC 2 cut(s) 633, 1165
Fsp4HI GCNGC 4 cut(s) 274, 766, 839, 1259
GluI GCNGC 4 cut(s) 274, 766, 839, 1259
GsuI CTGGAG 3 cut(s) 282, 642, 1347
HaeIII GGCC 4 cut(s) 267, 387, 890, 1261
HapII CCGG 2 cut(s) 982, 1350
Hin1II CATG 8 cut(s) 62, 362, 378, 810, 866, 1187, 1192, 1358
HincII GTYRAC 5 cut(s) 219, 364, 694, 723, 1315
HindII GTYRAC 5 cut(s) 219, 364, 694, 723, 1315
HinfI GANTC 7 cut(s) 179, 215, 407, 426, 560, 959, 1138
HpaII CCGG 2 cut(s) 982, 1350
HphI GGTGA 5 cut(s) 103, 188, 402, 785, 1190
Hpy188I TCNGA 5 cut(s) 235, 736, 932, 1297, 1427
Hpy188III TCNNGA 3 cut(s) 570, 1072, 1364
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 8 cut(s) 26, 125, 240, 301, 443, 590, 653, 1166
HpyCH4III ACNGT 4 cut(s) 1028, 1111, 1175, 1339
HpyCH4IV ACGT 3 cut(s) 780, 996, 1311
HpyCH4V TGCA 8 cut(s) 58, 374, 713, 824, 862, 971, 1327, 1334
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 508
HpyF3I CTNAG 4 cut(s) 697, 942, 1142, 1294
HpySE526I ACGT 3 cut(s) 780, 996, 1311
Hsp92II CATG 8 cut(s) 62, 362, 378, 810, 866, 1187, 1192, 1358
Ksp22I TGATCA 1 cut(s) 832
Kzo9I GATC 7 cut(s) 817, 832, 923, 999, 1221, 1228, 1399
LmnI GCTCC 2 cut(s) 628, 1366
Lsp1109I GCAGC 3 cut(s) 260, 752, 825
LweI GCATC 1 cut(s) 463
MabI ACCWGGT 1 cut(s) 121
MaeII ACGT 3 cut(s) 780, 996, 1311
MaeIII GTNAC 7 cut(s) 109, 176, 452, 611, 1111, 1145, 1178
MalI GATC 7 cut(s) 819, 834, 925, 1001, 1223, 1230, 1401
MboI GATC 7 cut(s) 817, 832, 923, 999, 1221, 1228, 1399
MboII GAAGA 8 cut(s) 248, 482, 682, 836, 920, 930, 933, 1087
MfeI CAATTG 2 cut(s) 194, 877
MflI RGATCY 2 cut(s) 817, 1221
MhlI GDGCHC 2 cut(s) 633, 1165
MluCI AATT 6 cut(s) 194, 573, 849, 877, 946, 1234
MlyI GAGTC 2 cut(s) 173, 224
Mph1103I ATGCAT 2 cut(s) 376, 864
MroXI GAANNNNTTC 2 cut(s) 15, 229
MseI TTAA 4 cut(s) 27, 783, 852, 1044
MslI CAYNNNNRTG 3 cut(s) 49, 373, 976
MspA1I CMGCKG 1 cut(s) 838
MspCI CTTAAG 1 cut(s) 26
MspI CCGG 2 cut(s) 982, 1350
MspR9I CCNGG 3 cut(s) 123, 270, 1214
MunI CAATTG 2 cut(s) 194, 877
MvaI CCWGG 3 cut(s) 123, 270, 1214
MwoI GCNNNNNNNGC 2 cut(s) 273, 508
NdeI CATATG 2 cut(s) 325, 370
NdeII GATC 7 cut(s) 817, 832, 923, 999, 1221, 1228, 1399
NlaIII CATG 8 cut(s) 62, 362, 378, 810, 866, 1187, 1192, 1358
NlaIV GGNNCC 9 cut(s) 6, 137, 630, 873, 980, 1210, 1223, 1348, 1362
NmuCI GTSAC 4 cut(s) 109, 176, 1111, 1178
NsiI ATGCAT 2 cut(s) 376, 864
NspI RCATGY 4 cut(s) 62, 810, 866, 1192
PciI ACATGT 1 cut(s) 1188
PdmI GAANNNNTTC 2 cut(s) 15, 229
PfeI GAWTC 5 cut(s) 407, 426, 560, 959, 1138
PkrI GCNGC 4 cut(s) 275, 767, 840, 1260
PleI GAGTC 2 cut(s) 173, 223
PpsI GAGTC 2 cut(s) 173, 223
Ppu21I YACGTR 1 cut(s) 997
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 1188
PshBI ATTAAT 1 cut(s) 852
Psp124BI GAGCTC 1 cut(s) 1165
Psp1406I AACGTT 1 cut(s) 1311
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 3 cut(s) 121, 268, 1212
PspEI GGTNACC 1 cut(s) 1178
PspGI CCWGG 3 cut(s) 121, 268, 1212
PspN4I GGNNCC 9 cut(s) 6, 137, 630, 873, 980, 1210, 1223, 1348, 1362
PspPI GGNCC 6 cut(s) 41, 136, 266, 308, 385, 481
PspPPI RGGWCCY 1 cut(s) 136
PsuI RGATCY 2 cut(s) 817, 1221
PvuII CAGCTG 1 cut(s) 838
RsaI GTAC 3 cut(s) 594, 986, 1370
RsaNI GTAC 3 cut(s) 593, 985, 1369
RseI CAYNNNNRTG 3 cut(s) 49, 373, 976
SacI GAGCTC 1 cut(s) 1165
SalI GTCGAC 1 cut(s) 217
SaqAI TTAA 4 cut(s) 27, 783, 852, 1044
SatI GCNGC 4 cut(s) 274, 766, 839, 1259
Sau3AI GATC 7 cut(s) 817, 832, 923, 999, 1221, 1228, 1399
Sau96I GGNCC 6 cut(s) 41, 136, 266, 308, 385, 481
SchI GAGTC 2 cut(s) 173, 224
ScrFI CCNGG 3 cut(s) 123, 270, 1214
SduI GDGCHC 2 cut(s) 633, 1165
SexAI ACCWGGT 1 cut(s) 121
SfaNI GCATC 1 cut(s) 463
SinI GGWCC 4 cut(s) 41, 136, 308, 481
SmiMI CAYNNNNRTG 3 cut(s) 49, 373, 976
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 6 cut(s) 194, 573, 849, 877, 946, 1234
SsiI CCGC 4 cut(s) 502, 768, 1033, 1258
SspI AATATT 2 cut(s) 530, 856
SstI GAGCTC 1 cut(s) 1165
StyD4I CCNGG 3 cut(s) 121, 268, 1212
TaaI ACNGT 4 cut(s) 1028, 1111, 1175, 1339
TaiI ACGT 3 cut(s) 783, 999, 1314
TaqI TCGA 3 cut(s) 218, 646, 1159
TaqII GACCGA 1 cut(s) 29
TasI AATT 6 cut(s) 194, 573, 849, 877, 946, 1234
TatI WGTACW 2 cut(s) 592, 1368
TauI GCSGC 1 cut(s) 1261
TfiI GAWTC 5 cut(s) 407, 426, 560, 959, 1138
Tru1I TTAA 4 cut(s) 27, 783, 852, 1044
Tru9I TTAA 4 cut(s) 27, 783, 852, 1044
TscAI CASTG 5 cut(s) 265, 427, 705, 1033, 1180
TseFI GTSAC 4 cut(s) 109, 176, 1111, 1178
TseI GCWGC 3 cut(s) 273, 765, 838
Tsp45I GTSAC 4 cut(s) 109, 176, 1111, 1178
TspDTI ATGAA 5 cut(s) 315, 347, 483, 673, 753
TspGWI ACGGA 1 cut(s) 1214
TspRI CASTG 5 cut(s) 265, 427, 705, 1033, 1180
Vha464I CTTAAG 1 cut(s) 26
VpaK11BI GGWCC 4 cut(s) 41, 136, 308, 481
VspI ATTAAT 1 cut(s) 852
XapI RAATTY 3 cut(s) 573, 946, 1234
XceI RCATGY 4 cut(s) 62, 810, 866, 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 3 cut(s) 218, 346, 789
XmnI GAANNNNTTC 2 cut(s) 15, 229
Zsp2I ATGCAT 2 cut(s) 376, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.