Rroxscaffold_5G00343270

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000839
Physical Location & Seq
Forward (+)
12564035 .. 12568482
4448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343270.1

Sequence Viewer

Length: 1386 bp
ATGGATTTTGTTCATCATTTGGCACCCACAATGAAAATGATGGATTTTGTTCCACTGTTAATGTTGGCTTTCACTTTGATACTCACTTCAACTTCAGCTGCCAGCATTACTACAACCAACATTACGATTCTACCGGGTTATTCCGGTGACCTTCCTTTTGCACTGGAATCCGGATATATCGGTGTTGGTGACAATGAAGAAGTGCAGCTATTTTATGTTTTCGTTGAGTCTGAGAGAGCTCCTGCAGACGATCCTCTGTTGCTATGGTTCAATGGAGGCCCAGGTTGCTCTGGTCTTGCTGGATTCTTCTTTGAGAGTGGTCCAGTAACTTTTAAGTTTGGAAATTACAATGGGAGCCTACCAGCAGTTCTTGACAATCCATTTCGATGGACAAAGGACGTCAGCATGATATACATAGATGCACCTGTTGGTGCTGGATTCTCCTACTCAAAAACTCAAAGCGGTTATGTTATGGATGACTATAAATATGTTGCACAAAGCTATGGATTTCTGCAGAAGTGGCTGGAGGCACACCCTGCATATTTGGAAAATGTACTCTACATTGGTGGTGACTCCTTCTCTGGCATAATTGTGCCAATGATTGTTCAACAAATATTGGATGGTAATGAAAATGGAACTTTGCCTCTAATGAATCTCAAGGGATATGTAATTGGGAACCCAGTAACGGATTCATTCATTGATGCAAATGCAAGAATTCCTTATGCCCATCGGCTAACTCTTATATCAGATCAACTGTATCAGGCGGCGAAAACAAGTTGCAGTGGAGATTACGTAAACGTAGATTCAAGCAATGACGCATGCATTTCAGATATCGATGCTATTGATGCACTCATAAATGACGTAAATACCTTACATGTCTTGGAACCAGTGTGTAGTGAAGGCACTGCCCGAAGATATCTCAAAGATTTGTCCGAAGGTTTACTAAGTTCAGCAACTACTGGTCCTGCATATTGGTGTAGGAGTTATAATCATATGCTCGCCTACAATTGGGCAAACCACCAAAGTGTTCAAGATGCTCTTGGCGTCCGGAGGGGCACAAAAAGATTTTGGCAATACTGTAATTCAACCCTGGCGTATACTCAAGAAGTCACTAGTGTTATTAGTCTTCATCAAAGTTTCGCGGATGTTGGCCTACGTGCTCTGATCTACAGTGGTGATCACGACATGACAATTCCTCACATTGCTACACAAAAATGGATAAACTTTCTCAATTTGACTCTGAATGAGACCTGGAGGGCATGGTCAGTTGATAACCAAACTGCAGGTTTGGAGCTAATGCAGGGAGGAGGCCACATTGCTGCAGAGTACAAGGTCAAAGAATGCTCTGCAATGATTGATAGATGGATGGCTCGGTCCCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

461

Amino Acids

50.98

Weight (kDa)

4.81

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 45 - 457 3.1e-105 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 987
AatII GACGTC 1 cut(s) 402
Acc36I ACCTGC 1 cut(s) 1274
AccB1I GGYRCC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 1097
AccII CGCG 1 cut(s) 1142
AccIII TCCGGA 2 cut(s) 170, 1047
AciI CCGC 3 cut(s) 462, 764, 1142
AclWI GGATC 1 cut(s) 245
AcsI RAATTY 1 cut(s) 714
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 399, 1044
AfaI GTAC 2 cut(s) 555, 1328
AfiI CCNNNNNNNGG 2 cut(s) 685, 1008
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 6 cut(s) 90, 271, 608, 807, 1031, 1086
AhlI ACTAGT 1 cut(s) 1112
AjnI CCWGG 3 cut(s) 280, 1089, 1250
AleI CACNNNNGTG 1 cut(s) 1023
AluBI AGCT 5 cut(s) 98, 208, 239, 501, 1294
AluI AGCT 5 cut(s) 98, 208, 239, 501, 1294
Alw21I GWGCWC 2 cut(s) 241, 1162
Alw26I GTCTC 1 cut(s) 1241
AlwI GGATC 1 cut(s) 245
Aor13HI TCCGGA 2 cut(s) 170, 1047
AoxI GGCC 3 cut(s) 277, 1150, 1309
ApeKI GCWGC 3 cut(s) 98, 205, 1319
ApoI RAATTY 1 cut(s) 714
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
AspS9I GGNCC 4 cut(s) 278, 320, 962, 1374
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 4 cut(s) 158, 200, 581, 1187
AvaII GGWCC 3 cut(s) 320, 962, 1374
BaeGI GKGCMC 1 cut(s) 1058
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 1118
Bbv12I GWGCWC 2 cut(s) 241, 1162
BbvI GCAGC 3 cut(s) 85, 217, 1306
BccI CCATC 6 cut(s) 34, 381, 614, 735, 1356, 1360
BciT130I CCWGG 3 cut(s) 282, 1091, 1252
BclI TGATCA 1 cut(s) 1177
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 1241
BcuI ACTAGT 1 cut(s) 1112
BfaI CTAG 1 cut(s) 1113
BfmI CTRYAG 5 cut(s) 243, 512, 1168, 1281, 1320
BfuAI ACCTGC 1 cut(s) 1274
BisI GCNGC 4 cut(s) 99, 206, 765, 1320
BlsI GCNGC 4 cut(s) 100, 207, 766, 1321
Bme1390I CCNGG 4 cut(s) 135, 282, 1091, 1252
Bme18I GGWCC 3 cut(s) 320, 962, 1374
BmgT120I GGNCC 4 cut(s) 278, 320, 962, 1374
BmiI GGNNCC 5 cut(s) 24, 356, 677, 885, 1376
BmrFI CCNGG 4 cut(s) 135, 282, 1091, 1252
BmrI ACTGGG 1 cut(s) 674
BmsI GCATC 5 cut(s) 409, 691, 826, 835, 1024
BmuI ACTGGG 1 cut(s) 674
BpiI GAAGAC 1 cut(s) 1118
BpmI CTGGAG 2 cut(s) 545, 1273
BpuEI CTTGAG 2 cut(s) 641, 1086
BpuMI CCSGG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 834
BsaAI YACGTR 2 cut(s) 793, 1157
BsaHI GRCGYC 2 cut(s) 399, 1044
BsaI GGTCTC 1 cut(s) 1241
BsaJI CCNNGG 2 cut(s) 280, 1089
BsaWI WCCGGW 3 cut(s) 143, 170, 1047
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 2 cut(s) 685, 1008
Bse1I ACTGG 5 cut(s) 168, 323, 680, 887, 964
Bse3DI GCAATG 4 cut(s) 817, 1200, 1314, 1356
BseAI TCCGGA 2 cut(s) 170, 1047
BseBI CCWGG 3 cut(s) 282, 1091, 1252
BseCI ATCGAT 1 cut(s) 834
BseDI CCNNGG 2 cut(s) 280, 1089
BseGI GGATG 4 cut(s) 481, 625, 1150, 1371
BseLI CCNNNNNNNGG 2 cut(s) 685, 1008
BseMI GCAATG 4 cut(s) 817, 1200, 1314, 1356
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 5 cut(s) 168, 323, 680, 887, 964
BseRI GAGGAG 1 cut(s) 1320
BseSI GKGCMC 1 cut(s) 1058
BseXI GCAGC 3 cut(s) 85, 217, 1306
BsgI GTGCAG 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 1142
BshFI GGCC 3 cut(s) 279, 1152, 1311
BshNI GGYRCC 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 834
BsiHKAI GWGCWC 2 cut(s) 241, 1162
BsiSI CCGG 4 cut(s) 134, 144, 171, 1048
BslFI GGGAC 1 cut(s) 1360
BslI CCNNNNNNNGG 2 cut(s) 685, 1008
BsmAI GTCTC 1 cut(s) 1241
BsmFI GGGAC 1 cut(s) 1360
BsmI GAATGC 1 cut(s) 1346
BsnI GGCC 3 cut(s) 279, 1152, 1311
Bso31I GGTCTC 1 cut(s) 1241
Bsp1286I GDGCHC 3 cut(s) 241, 1058, 1162
Bsp13I TCCGGA 2 cut(s) 170, 1047
Bsp143I GATC 4 cut(s) 250, 748, 1164, 1177
BspACI CCGC 3 cut(s) 462, 764, 1142
BspANI GGCC 3 cut(s) 279, 1152, 1311
BspCNI CTCAG 1 cut(s) 223
BspDI ATCGAT 1 cut(s) 834
BspEI TCCGGA 2 cut(s) 170, 1047
BspFNI CGCG 1 cut(s) 1142
BspLI GGNNCC 5 cut(s) 24, 356, 677, 885, 1376
BspMAI CTGCAG 4 cut(s) 247, 516, 1285, 1324
BspMI ACCTGC 1 cut(s) 1274
BspPI GGATC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 1241
BsrDI GCAATG 4 cut(s) 817, 1200, 1314, 1356
BsrI ACTGG 5 cut(s) 168, 323, 680, 887, 964
BssECI CCNNGG 2 cut(s) 280, 1089
BssMI GATC 4 cut(s) 250, 748, 1164, 1177
BssNAI GTATAC 1 cut(s) 1098
BssNI GRCGYC 2 cut(s) 399, 1044
Bst1107I GTATAC 1 cut(s) 1098
Bst2UI CCWGG 3 cut(s) 282, 1091, 1252
Bst4CI ACNGT 4 cut(s) 57, 756, 1079, 1172
BstACI GRCGYC 2 cut(s) 399, 1044
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBAI YACGTR 2 cut(s) 793, 1157
BstC8I GCNNGC 3 cut(s) 103, 820, 999
BstDEI CTNAG 2 cut(s) 231, 944
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 4 cut(s) 481, 625, 1150, 1371
BstFNI CGCG 1 cut(s) 1142
BstKTI GATC 4 cut(s) 253, 751, 1167, 1180
BstMAI GTCTC 1 cut(s) 1241
BstMBI GATC 4 cut(s) 250, 748, 1164, 1177
BstMWI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
BstNI CCWGG 3 cut(s) 282, 1091, 1252
BstNSI RCATGY 2 cut(s) 822, 878
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 4 cut(s) 133, 280, 1089, 1250
BstSFI CTRYAG 5 cut(s) 243, 512, 1168, 1281, 1320
BstSLI GKGCMC 1 cut(s) 1058
BstSNI TACGTA 1 cut(s) 793
BstUI CGCG 1 cut(s) 1142
BstV1I GCAGC 3 cut(s) 85, 217, 1306
BstV2I GAAGAC 1 cut(s) 1118
BstXI CCANNNNNNTGG 1 cut(s) 387
BstZ17I GTATAC 1 cut(s) 1098
Bsu15I ATCGAT 1 cut(s) 834
BsuRI GGCC 3 cut(s) 279, 1152, 1311
BsuTUI ATCGAT 1 cut(s) 834
BtsCI GGATG 4 cut(s) 481, 625, 1150, 1371
BtsI GCAGTG 2 cut(s) 787, 903
BtsIMutI CAGTG 6 cut(s) 53, 161, 787, 894, 903, 1177
BveI ACCTGC 1 cut(s) 1274
Cac8I GCNNGC 3 cut(s) 103, 820, 999
Cfr13I GGNCC 4 cut(s) 278, 320, 962, 1374
ClaI ATCGAT 1 cut(s) 834
CseI GACGC 2 cut(s) 824, 1033
Csp6I GTAC 2 cut(s) 554, 1327
CviAII CATG 5 cut(s) 406, 819, 875, 1186, 1260
CviQI GTAC 2 cut(s) 554, 1327
DdeI CTNAG 2 cut(s) 231, 944
DpnI GATC 4 cut(s) 252, 750, 1166, 1179
DpnII GATC 4 cut(s) 250, 748, 1164, 1177
Ecl136II GAGCTC 1 cut(s) 239
Eco105I TACGTA 1 cut(s) 793
Eco24I GRGCYC 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 1241
Eco32I GATATC 2 cut(s) 832, 917
Eco47I GGWCC 3 cut(s) 320, 962, 1374
Eco53kI GAGCTC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 3 cut(s) 280, 1089, 1250
EcoRV GATATC 2 cut(s) 832, 917
EcoT22I ATGCAT 1 cut(s) 824
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 5 cut(s) 409, 822, 878, 1189, 1263
FalI AAGNNNNNCTT 4 cut(s) 703, 735, 1023, 1055
FaqI GGGAC 1 cut(s) 1360
FatI CATG 5 cut(s) 405, 818, 874, 1185, 1259
FauNDI CATATG 1 cut(s) 993
FbaI TGATCA 1 cut(s) 1177
FblI GTMKAC 1 cut(s) 1097
Fnu4HI GCNGC 4 cut(s) 99, 206, 765, 1320
FokI GGATG 4 cut(s) 488, 632, 1157, 1378
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 4 cut(s) 99, 206, 765, 1320
FspBI CTAG 1 cut(s) 1113
GluI GCNGC 4 cut(s) 99, 206, 765, 1320
GsuI CTGGAG 2 cut(s) 545, 1273
HaeIII GGCC 3 cut(s) 279, 1152, 1311
HapII CCGG 4 cut(s) 134, 144, 171, 1048
HgaI GACGC 2 cut(s) 824, 1033
Hin1I GRCGYC 2 cut(s) 399, 1044
Hin1II CATG 5 cut(s) 409, 822, 878, 1189, 1263
HpaII CCGG 4 cut(s) 134, 144, 171, 1048
HphI GGTGA 4 cut(s) 158, 200, 581, 1187
Hpy166II GTNNAC 3 cut(s) 796, 941, 1098
Hpy188I TCNGA 6 cut(s) 232, 748, 829, 934, 1164, 1242
Hpy188III TCNNGA 6 cut(s) 171, 371, 1031, 1048, 1103, 1181
Hpy8I GTNNAC 3 cut(s) 796, 941, 1098
HpyAV CCTTC 4 cut(s) 161, 586, 893, 929
HpyCH4III ACNGT 4 cut(s) 57, 756, 1079, 1172
HpyCH4IV ACGT 5 cut(s) 399, 792, 798, 861, 1156
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
HpyF3I CTNAG 2 cut(s) 231, 944
HpySE526I ACGT 5 cut(s) 399, 792, 798, 861, 1156
Hsp92I GRCGYC 2 cut(s) 399, 1044
Hsp92II CATG 5 cut(s) 409, 822, 878, 1189, 1263
Kpn2I TCCGGA 2 cut(s) 170, 1047
Ksp22I TGATCA 1 cut(s) 1177
Kzo9I GATC 4 cut(s) 250, 748, 1164, 1177
LmnI GCTCC 3 cut(s) 244, 354, 1291
Lsp1109I GCAGC 3 cut(s) 85, 217, 1306
LweI GCATC 5 cut(s) 409, 691, 826, 835, 1024
MaeI CTAG 1 cut(s) 1113
MaeII ACGT 5 cut(s) 399, 792, 798, 861, 1156
MaeIII GTNAC 6 cut(s) 146, 188, 325, 569, 682, 1108
MalI GATC 4 cut(s) 252, 750, 1166, 1179
MboI GATC 4 cut(s) 250, 748, 1164, 1177
MboII GAAGA 4 cut(s) 209, 298, 924, 1118
MfeI CAATTG 1 cut(s) 1006
MhlI GDGCHC 3 cut(s) 241, 1058, 1162
MluCI AATT 8 cut(s) 343, 588, 669, 714, 1006, 1081, 1191, 1231
MlyI GAGTC 3 cut(s) 236, 566, 1231
MnlI CCTC 9 cut(s) 264, 269, 520, 654, 1044, 1206, 1248, 1298, 1301
Mph1103I ATGCAT 1 cut(s) 824
MroI TCCGGA 2 cut(s) 170, 1047
MseI TTAA 2 cut(s) 59, 333
MslI CAYNNNNRTG 5 cut(s) 385, 590, 973, 1023, 1213
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 4 cut(s) 134, 144, 171, 1048
MspR9I CCNGG 4 cut(s) 135, 282, 1091, 1252
MunI CAATTG 1 cut(s) 1006
Mva1269I GAATGC 1 cut(s) 1346
MvaI CCWGG 3 cut(s) 282, 1091, 1252
MvnI CGCG 1 cut(s) 1142
MwoI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
NciI CCSGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 993
NdeII GATC 4 cut(s) 250, 748, 1164, 1177
NlaIII CATG 5 cut(s) 409, 822, 878, 1189, 1263
NlaIV GGNNCC 5 cut(s) 24, 356, 677, 885, 1376
NmuCI GTSAC 4 cut(s) 146, 188, 569, 1108
NsiI ATGCAT 1 cut(s) 824
NspI RCATGY 2 cut(s) 822, 878
OliI CACNNNNGTG 1 cut(s) 1023
PaeI GCATGC 1 cut(s) 822
PciI ACATGT 1 cut(s) 874
PctI GAATGC 1 cut(s) 1346
PfeI GAWTC 7 cut(s) 127, 167, 303, 438, 652, 689, 803
PkrI GCNGC 4 cut(s) 100, 207, 766, 1321
PleI GAGTC 3 cut(s) 235, 566, 1231
PpsI GAGTC 3 cut(s) 235, 566, 1231
Ppu21I YACGTR 2 cut(s) 793, 1157
PscI ACATGT 1 cut(s) 874
PsiI TTATAA 1 cut(s) 987
Psp124BI GAGCTC 1 cut(s) 241
Psp6I CCWGG 3 cut(s) 280, 1089, 1250
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 3 cut(s) 280, 1089, 1250
PspN4I GGNNCC 5 cut(s) 24, 356, 677, 885, 1376
PspPI GGNCC 4 cut(s) 278, 320, 962, 1374
PstI CTGCAG 4 cut(s) 247, 516, 1285, 1324
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 2 cut(s) 555, 1328
RsaNI GTAC 2 cut(s) 554, 1327
RseI CAYNNNNRTG 5 cut(s) 385, 590, 973, 1023, 1213
SacI GAGCTC 1 cut(s) 241
SaqAI TTAA 2 cut(s) 59, 333
SatI GCNGC 4 cut(s) 99, 206, 765, 1320
Sau3AI GATC 4 cut(s) 250, 748, 1164, 1177
Sau96I GGNCC 4 cut(s) 278, 320, 962, 1374
SchI GAGTC 3 cut(s) 236, 566, 1231
ScrFI CCNGG 4 cut(s) 135, 282, 1091, 1252
SduI GDGCHC 3 cut(s) 241, 1058, 1162
SfaNI GCATC 5 cut(s) 409, 691, 826, 835, 1024
SfcI CTRYAG 5 cut(s) 243, 512, 1168, 1281, 1320
SinI GGWCC 3 cut(s) 320, 962, 1374
SmiMI CAYNNNNRTG 5 cut(s) 385, 590, 973, 1023, 1213
SmlI CTYRAG 2 cut(s) 656, 1101
SmoI CTYRAG 2 cut(s) 656, 1101
SnaBI TACGTA 1 cut(s) 793
SpeI ACTAGT 1 cut(s) 1112
SphI GCATGC 1 cut(s) 822
Sse9I AATT 8 cut(s) 343, 588, 669, 714, 1006, 1081, 1191, 1231
SsiI CCGC 3 cut(s) 462, 764, 1142
SspI AATATT 1 cut(s) 615
SspMI CTAG 1 cut(s) 1113
SstI GAGCTC 1 cut(s) 241
StyD4I CCNGG 4 cut(s) 133, 280, 1089, 1250
TaaI ACNGT 4 cut(s) 57, 756, 1079, 1172
TaiI ACGT 5 cut(s) 402, 795, 801, 864, 1159
TaqI TCGA 2 cut(s) 385, 834
TaqII GACCGA 1 cut(s) 1362
TasI AATT 8 cut(s) 343, 588, 669, 714, 1006, 1081, 1191, 1231
TatI WGTACW 2 cut(s) 553, 1326
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 7 cut(s) 127, 167, 303, 438, 652, 689, 803
Tru1I TTAA 2 cut(s) 59, 333
Tru9I TTAA 2 cut(s) 59, 333
TscAI CASTG 6 cut(s) 60, 168, 787, 894, 910, 1177
TseFI GTSAC 4 cut(s) 146, 188, 569, 1108
TseI GCWGC 3 cut(s) 98, 205, 1319
Tsp45I GTSAC 4 cut(s) 146, 188, 569, 1108
TspDTI ATGAA 7 cut(s) 47, 210, 642, 665, 681, 685, 1118
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 6 cut(s) 60, 168, 787, 894, 910, 1177
VpaK11BI GGWCC 3 cut(s) 320, 962, 1374
XapI RAATTY 1 cut(s) 714
XceI RCATGY 2 cut(s) 822, 878
XmiI GTMKAC 1 cut(s) 1097
XspI CTAG 1 cut(s) 1113
ZraI GACGTC 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.