Rorug04G0007400

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000839
Physical Location & Seq
Forward (+)
1315390 .. 1316494
1105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0007400.1

Sequence Viewer

Length: 1008 bp
ATGCCTCTAAATAGCTTTCCTTCCACTGTCTCTAGAGAGACTTTAGAGCTCAAGCTTAAGGCTGCGGAAAGCAGAATTTACGTTGATGTTGGTTTCTGGGGAGGCCTTGTTCCTGAAAATGCATTCAACAAATCTGCTATTGAGGATCTCTTAAATGCCGGTGCTCTTGGTTTGAAGTCTTTCATGTGTCCATCAGGGATCAATGACTTTCCTATGACAAACAGCAGTCATATTAAGGAGGGTCTGTTTGTACTGGCAAAATATCGAAGGCCTTTATTTGTACATGCAGAGAAACAACTAGATCCTGAAACTAACTTGGGAGTCGAAGATGGTAGTAATGATGCTCGTTCCTATTCGACGTATCTTAAGACTAGACCACCTTCATGGGAAGAGGTAGCTATAAAGGATCTCGTTACCTTGACAAAGGACACAAGGATCGGTGCTCCTGCAGAAGGAGCTCATCTTCATATTGTGCACTTGTCTGACTCAAGCTCTTCATTAGATCTTATTAAGGAAGCAAAAAGTGGTGGCGATAGTTTAACTGTTGAGACGTGCCCCCACTACTTAGCTTTCTCCGCCGAAGAGATTCGGGATGGAGATACTCGTTTTAAGTGTTCTCCGCCCATCCTTGATGCTGTCAATAAGGAAAAACTGTGGAAGGCTTTGTTGGAAGGGGATCTTGACATGTTAAGCTCTGATCATTCTCCAACAACACCCGAACTTAAGCGTCTGGATGATGGTGACTTTTTAAAGGCTTGGGGTGGTATATCGTGCTTGCAGTTTGTTCTTCCTGTGACATGGTCGTATGGCCAGATATATGGAGTGCCTTTAGAACAAATGGCTCTGTGGTGGAGTGAAAGGTCGGCCATGCTTGCTGGACAAAAACTAAAGGGGGCAATTGCAATTGGAAACTATGCAGATATTGTGGTATGGAATCCAGATGTTGATTTTGACCTTGACGATGACTATCCTATACACATTAAGCATCCGATCATGTTGTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

335

Amino Acids

37.13

Weight (kDa)

5.03

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 156 - 321 1.1e-06 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 65, 576, 620
AclWI GGATC 6 cut(s) 153, 206, 296, 414, 443, 684
AcoI YGGCCR 2 cut(s) 808, 864
AcsI RAATTY 1 cut(s) 75
AfaI GTAC 2 cut(s) 252, 282
AfiI CCNNNNNNNGG 1 cut(s) 452
AflII CTTAAG 3 cut(s) 56, 365, 722
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 3 cut(s) 127, 175, 1003
AjiI CACGTC 1 cut(s) 552
AjuI GAANNNNNNNTTGG 2 cut(s) 650, 682
AloI GAACNNNNNNTCC 2 cut(s) 93, 125
AluBI AGCT 8 cut(s) 15, 49, 55, 398, 458, 492, 569, 693
AluI AGCT 8 cut(s) 15, 49, 55, 398, 458, 492, 569, 693
Alw21I GWGCWC 5 cut(s) 51, 166, 445, 460, 477
Alw26I GTCTC 3 cut(s) 32, 34, 542
Alw44I GTGCAC 1 cut(s) 473
AlwI GGATC 6 cut(s) 153, 206, 296, 414, 443, 684
AoxI GGCC 4 cut(s) 103, 269, 808, 864
ApaLI GTGCAC 1 cut(s) 473
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 1 cut(s) 75
Asp700I GAANNNNTTC 2 cut(s) 179, 585
AsuHPI GGTGA 1 cut(s) 752
BaeGI GKGCMC 2 cut(s) 477, 557
BalI TGGCCA 1 cut(s) 810
BanII GRGCYC 2 cut(s) 51, 460
Bbv12I GWGCWC 5 cut(s) 51, 166, 445, 460, 477
BbvI GCAGC 1 cut(s) 49
BccI CCATC 5 cut(s) 199, 323, 587, 632, 731
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 3 cut(s) 32, 34, 542
BfaI CTAG 3 cut(s) 33, 299, 372
BfmI CTRYAG 1 cut(s) 447
BfrI CTTAAG 3 cut(s) 56, 365, 722
BglII AGATCT 1 cut(s) 502
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
BmgBI CACGTC 1 cut(s) 552
BmsI GCATC 3 cut(s) 331, 622, 994
BpuEI CTTGAG 2 cut(s) 35, 472
BsaBI GATNNNNATC 1 cut(s) 966
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 230, 260
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 966
BseGI GGATG 4 cut(s) 598, 624, 739, 985
BseJI GATNNNNATC 1 cut(s) 966
BseLI CCNNNNNNNGG 1 cut(s) 452
BseNI ACTGG 1 cut(s) 258
BseSI GKGCMC 2 cut(s) 477, 557
BseXI GCAGC 1 cut(s) 49
BshFI GGCC 4 cut(s) 105, 271, 810, 866
BsiHKAI GWGCWC 5 cut(s) 51, 166, 445, 460, 477
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 452
BsmAI GTCTC 3 cut(s) 32, 34, 542
BsmBI CGTCTC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 4 cut(s) 105, 271, 810, 866
Bsp1286I GDGCHC 6 cut(s) 51, 166, 445, 460, 477, 557
Bsp1407I TGTACA 1 cut(s) 280
Bsp143I GATC 9 cut(s) 145, 198, 301, 406, 435, 502, 676, 697, 990
BspACI CCGC 3 cut(s) 65, 576, 620
BspANI GGCC 4 cut(s) 105, 271, 810, 866
BspMAI CTGCAG 1 cut(s) 451
BspPI GGATC 6 cut(s) 153, 206, 296, 414, 443, 684
BspQI GCTCTTC 1 cut(s) 499
BspTI CTTAAG 3 cut(s) 56, 365, 722
BsrFI RCCGGY 1 cut(s) 158
BsrGI TGTACA 1 cut(s) 280
BsrI ACTGG 1 cut(s) 258
BssAI RCCGGY 1 cut(s) 158
BssMI GATC 9 cut(s) 145, 198, 301, 406, 435, 502, 676, 697, 990
Bst4CI ACNGT 3 cut(s) 28, 544, 654
Bst6I CTCTTC 3 cut(s) 384, 499, 576
BstAFI CTTAAG 3 cut(s) 56, 365, 722
BstAUI TGTACA 1 cut(s) 280
BstC8I GCNNGC 2 cut(s) 776, 873
BstDEI CTNAG 1 cut(s) 565
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 4 cut(s) 598, 624, 739, 985
BstKTI GATC 9 cut(s) 148, 201, 304, 409, 438, 505, 679, 700, 993
BstMAI GTCTC 3 cut(s) 32, 34, 542
BstMBI GATC 9 cut(s) 145, 198, 301, 406, 435, 502, 676, 697, 990
BstMWI GCNNNNNNNGC 3 cut(s) 455, 575, 872
BstNSI RCATGY 2 cut(s) 287, 688
BstSFI CTRYAG 1 cut(s) 447
BstSLI GKGCMC 2 cut(s) 477, 557
BstV1I GCAGC 1 cut(s) 49
BstX2I RGATCY 5 cut(s) 145, 301, 406, 502, 676
BstXI CCANNNNNNTGG 2 cut(s) 384, 818
BstYI RGATCY 5 cut(s) 145, 301, 406, 502, 676
BsuRI GGCC 4 cut(s) 105, 271, 810, 866
BtrI CACGTC 1 cut(s) 552
BtsCI GGATG 4 cut(s) 598, 624, 739, 985
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 2 cut(s) 776, 873
Cfr10I RCCGGY 1 cut(s) 158
CseI GACGC 1 cut(s) 716
Csp6I GTAC 2 cut(s) 251, 281
CviAII CATG 7 cut(s) 184, 284, 384, 685, 798, 868, 994
CviQI GTAC 2 cut(s) 251, 281
DdeI CTNAG 1 cut(s) 565
DpnI GATC 9 cut(s) 147, 200, 303, 408, 437, 504, 678, 699, 992
DpnII GATC 9 cut(s) 145, 198, 301, 406, 435, 502, 676, 697, 990
DraI TTTAAA 1 cut(s) 750
EaeI YGGCCR 2 cut(s) 808, 864
Eam1104I CTCTTC 3 cut(s) 384, 499, 576
EarI CTCTTC 3 cut(s) 384, 499, 576
EciI GGCGGA 2 cut(s) 565, 609
Ecl136II GAGCTC 2 cut(s) 49, 458
Eco147I AGGCCT 2 cut(s) 105, 271
Eco24I GRGCYC 2 cut(s) 51, 460
Eco53kI GAGCTC 2 cut(s) 49, 458
EcoICRI GAGCTC 2 cut(s) 49, 458
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoT22I ATGCAT 1 cut(s) 124
EcoT38I GRGCYC 2 cut(s) 51, 460
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 7 cut(s) 187, 287, 387, 688, 801, 871, 997
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FatI CATG 7 cut(s) 183, 283, 383, 684, 797, 867, 993
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 4 cut(s) 605, 611, 746, 972
FriOI GRGCYC 2 cut(s) 51, 460
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 3 cut(s) 33, 299, 372
GluI GCNGC 1 cut(s) 63
HaeIII GGCC 4 cut(s) 105, 271, 810, 866
HapII CCGG 1 cut(s) 159
HgaI GACGC 1 cut(s) 716
Hin1II CATG 7 cut(s) 187, 287, 387, 688, 801, 871, 997
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 4 cut(s) 321, 485, 586, 934
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 752
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 3 cut(s) 484, 697, 990
Hpy188III TCNNGA 7 cut(s) 33, 113, 305, 590, 680, 731, 938
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 6 cut(s) 30, 261, 390, 446, 652, 665
HpyCH4III ACNGT 3 cut(s) 28, 544, 654
HpyCH4IV ACGT 3 cut(s) 81, 359, 551
HpyCH4V TGCA 7 cut(s) 122, 287, 449, 475, 778, 902, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 575, 872
HpyF3I CTNAG 1 cut(s) 565
HpySE526I ACGT 3 cut(s) 81, 359, 551
Hsp92II CATG 7 cut(s) 187, 287, 387, 688, 801, 871, 997
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 9 cut(s) 145, 198, 301, 406, 435, 502, 676, 697, 990
LguI GCTCTTC 1 cut(s) 499
LmnI GCTCC 2 cut(s) 448, 455
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 3 cut(s) 331, 622, 994
MaeI CTAG 3 cut(s) 33, 299, 372
MaeII ACGT 3 cut(s) 81, 359, 551
MaeIII GTNAC 3 cut(s) 412, 740, 793
MalI GATC 9 cut(s) 147, 200, 303, 408, 437, 504, 678, 699, 992
MboI GATC 9 cut(s) 145, 198, 301, 406, 435, 502, 676, 697, 990
MboII GAAGA 6 cut(s) 338, 401, 455, 486, 593, 779
MfeI CAATTG 2 cut(s) 897, 903
MflI RGATCY 5 cut(s) 145, 301, 406, 502, 676
MhlI GDGCHC 6 cut(s) 51, 166, 445, 460, 477, 557
MlsI TGGCCA 1 cut(s) 810
MluCI AATT 3 cut(s) 75, 897, 903
MluNI TGGCCA 1 cut(s) 810
MlyI GAGTC 2 cut(s) 330, 479
MmeI TCCRAC 2 cut(s) 648, 731
MnlI CCTC 5 cut(s) 15, 95, 136, 232, 385
Mox20I TGGCCA 1 cut(s) 810
Mph1103I ATGCAT 1 cut(s) 124
MroXI GAANNNNTTC 2 cut(s) 179, 585
MscI TGGCCA 1 cut(s) 810
MslI CAYNNNNRTG 1 cut(s) 382
Msp20I TGGCCA 1 cut(s) 810
MspCI CTTAAG 3 cut(s) 56, 365, 722
MspI CCGG 1 cut(s) 159
MunI CAATTG 2 cut(s) 897, 903
Mva1269I GAATGC 1 cut(s) 122
MwoI GCNNNNNNNGC 3 cut(s) 455, 575, 872
NdeII GATC 9 cut(s) 145, 198, 301, 406, 435, 502, 676, 697, 990
NlaIII CATG 7 cut(s) 187, 287, 387, 688, 801, 871, 997
NmuCI GTSAC 2 cut(s) 740, 793
NsiI ATGCAT 1 cut(s) 124
NspI RCATGY 2 cut(s) 287, 688
PceI AGGCCT 2 cut(s) 105, 271
PciI ACATGT 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 499
PctI GAATGC 1 cut(s) 122
PdmI GAANNNNTTC 2 cut(s) 179, 585
PfeI GAWTC 2 cut(s) 586, 934
PflFI GACNNNGTC 1 cut(s) 799
PkrI GCNGC 1 cut(s) 64
PleI GAGTC 2 cut(s) 329, 479
PpsI GAGTC 2 cut(s) 329, 479
PscI ACATGT 1 cut(s) 684
Psp124BI GAGCTC 2 cut(s) 51, 460
PstI CTGCAG 1 cut(s) 451
PsuI RGATCY 5 cut(s) 145, 301, 406, 502, 676
PsyI GACNNNGTC 1 cut(s) 799
RsaI GTAC 2 cut(s) 252, 282
RsaNI GTAC 2 cut(s) 251, 281
RseI CAYNNNNRTG 1 cut(s) 382
SacI GAGCTC 2 cut(s) 51, 460
SapI GCTCTTC 1 cut(s) 499
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 9 cut(s) 145, 198, 301, 406, 435, 502, 676, 697, 990
SchI GAGTC 2 cut(s) 330, 479
SduI GDGCHC 6 cut(s) 51, 166, 445, 460, 477, 557
SfaNI GCATC 3 cut(s) 331, 622, 994
SfcI CTRYAG 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 382
SmlI CTYRAG 5 cut(s) 50, 56, 365, 487, 722
SmoI CTYRAG 5 cut(s) 50, 56, 365, 487, 722
Sse9I AATT 3 cut(s) 75, 897, 903
SseBI AGGCCT 2 cut(s) 105, 271
SsiI CCGC 3 cut(s) 65, 576, 620
SspMI CTAG 3 cut(s) 33, 299, 372
SstI GAGCTC 2 cut(s) 51, 460
StuI AGGCCT 2 cut(s) 105, 271
TaaI ACNGT 3 cut(s) 28, 544, 654
TaiI ACGT 3 cut(s) 84, 362, 554
TaqI TCGA 3 cut(s) 265, 324, 356
TasI AATT 3 cut(s) 75, 897, 903
TatI WGTACW 2 cut(s) 250, 280
TfiI GAWTC 2 cut(s) 586, 934
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 2 cut(s) 740, 793
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 2 cut(s) 740, 793
TspDTI ATGAA 4 cut(s) 172, 372, 455, 486
TspRI CASTG 1 cut(s) 31
Tth111I GACNNNGTC 1 cut(s) 799
Vha464I CTTAAG 3 cut(s) 56, 365, 722
VneI GTGCAC 1 cut(s) 473
XagI CCTNNNNNAGG 1 cut(s) 450
XapI RAATTY 1 cut(s) 75
XbaI TCTAGA 1 cut(s) 32
XceI RCATGY 2 cut(s) 287, 688
XmnI GAANNNNTTC 2 cut(s) 179, 585
XspI CTAG 3 cut(s) 33, 299, 372
Zsp2I ATGCAT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.