Rh4BG082200

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000839
Physical Location & Seq
Forward (+)
14322855 .. 14325600
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG082200.1

Sequence Viewer

Length: 1071 bp
ATGGATTTTGTTCTTCATTTGGTACCCACAATGAAAATGATGGATTGTCTTCTACTGTTAATGTTGGCTTTCACTTTGATACTCACTTCAACTTCAGCTGCCAGCATTACTACAACCAACATTACGATTCTACCGGGTTATTCCGGTGACCTTCCTTTTGCACTGGAATCCGGATATATCGGTGTAGGTGACAATGAAGAAGTGCAGCTATTTTACGTTTTCGTTGAGTCTGAGAGAGCTCCTGCAGACGATCCTCTGTTGCTATGGTTCAATGGAGGCCCAGGTTGCTCTGGTCTTGCTGGATTCTTCTTTGAGAGTGGTCCAGTAACTTTTAAGTTTGGAAATTACAATGGGAGCCTACCAGCAGTTCTTGACAATCCATTTCGATGGACAAAGGACGTCAGCATGATATACATAGATGCACCTGTTGGTGCTGGATTCTCCTACTCAAAAACTCAAAGCGGTTATGTTATGGATGACTATAAATATGTTGCACAAAGCTATGAATTTCTGCAGAAGTGGCTGGAGGCACACCCTGCATATTTGGAAAATATGCTCTACATTGGTGGTGACTCCTTCTCTGGCATAATTGTGCCAATGATTGTGCAACAAATATTGGATGGTAATGAAAATGGAACTTTGCCTCTAATGAATCTCAAGGGATATGTAATCGGGAACCCAGTAACGGATTCATTCATTGATGCAAATGCAAGAATTCCTTATGCCCATCGGCTAACTCTTATATCAGATCAACTGTATCAGGCGGCGAAAACAAGTTGCAGTGGAGATTACGTAAACGTAGATTCAAGCAATGACGCATGCATTTCAGATATCGATGCTATTGATGCACTCATAAATGACGTAAATACCTTACACGTCTTGGAACCAGTGTGTAGTGAAGGCACTGCCCGAAGATATCTCAAAGATTTGTCCGAAGGTTTACTAAGTTCTGCAACTACTGGTCCTGCATATTGGTGTAGGGTAAGCCTCTTTCTGCTAGAGCATTCTTTTGTACAAACAGATGTGTACAATGCTCCTCTCCCTATAACTACTCATGCAATCAACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

356

Amino Acids

39.05

Weight (kDa)

4.36

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 45 - 307 4.2e-80 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 402
Acc65I GGTACC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 2 cut(s) 462, 764
AclWI GGATC 1 cut(s) 245
AcsI RAATTY 2 cut(s) 506, 714
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 3 cut(s) 24, 1014, 1028
AfiI CCNNNNNNNGG 1 cut(s) 685
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 3 cut(s) 90, 271, 807
AjiI CACGTC 1 cut(s) 877
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 4 cut(s) 98, 208, 239, 501
AluI AGCT 4 cut(s) 98, 208, 239, 501
Alw21I GWGCWC 1 cut(s) 241
AlwI GGATC 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 98, 205
ApoI RAATTY 2 cut(s) 506, 714
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
Asp718I GGTACC 1 cut(s) 22
AspS9I GGNCC 3 cut(s) 278, 320, 962
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 3 cut(s) 158, 200, 581
AvaII GGWCC 2 cut(s) 320, 962
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 2 cut(s) 85, 217
BccI CCATC 4 cut(s) 34, 381, 614, 735
BciT130I CCWGG 1 cut(s) 282
BcnI CCSGG 1 cut(s) 135
BfaI CTAG 1 cut(s) 998
BfmI CTRYAG 2 cut(s) 243, 512
BisI GCNGC 3 cut(s) 99, 206, 765
BlsI GCNGC 3 cut(s) 100, 207, 766
Bme1390I CCNGG 2 cut(s) 135, 282
Bme18I GGWCC 2 cut(s) 320, 962
BmgBI CACGTC 1 cut(s) 877
BmgT120I GGNCC 3 cut(s) 278, 320, 962
BmiI GGNNCC 4 cut(s) 24, 356, 677, 885
BmrFI CCNGG 2 cut(s) 135, 282
BmrI ACTGGG 1 cut(s) 674
BmsI GCATC 4 cut(s) 409, 691, 826, 835
BmuI ACTGGG 1 cut(s) 674
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 641
BpuMI CCSGG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 834
BsaAI YACGTR 1 cut(s) 793
BsaHI GRCGYC 1 cut(s) 399
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 2 cut(s) 143, 170
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 1 cut(s) 685
Bse1I ACTGG 5 cut(s) 168, 323, 680, 887, 964
Bse3DI GCAATG 1 cut(s) 817
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 1 cut(s) 282
BseCI ATCGAT 1 cut(s) 834
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 2 cut(s) 481, 625
BseLI CCNNNNNNNGG 1 cut(s) 685
BseMI GCAATG 1 cut(s) 817
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 5 cut(s) 168, 323, 680, 887, 964
BseRI GAGGAG 1 cut(s) 1026
BseXI GCAGC 2 cut(s) 85, 217
BsgI GTGCAG 1 cut(s) 224
BshFI GGCC 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 834
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 3 cut(s) 134, 144, 171
BslI CCNNNNNNNGG 1 cut(s) 685
BsmI GAATGC 1 cut(s) 1003
BsnI GGCC 1 cut(s) 279
Bsp1286I GDGCHC 1 cut(s) 241
Bsp13I TCCGGA 1 cut(s) 170
Bsp1407I TGTACA 2 cut(s) 1012, 1026
Bsp143I GATC 2 cut(s) 250, 748
BspACI CCGC 2 cut(s) 462, 764
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 1 cut(s) 223
BspDI ATCGAT 1 cut(s) 834
BspEI TCCGGA 1 cut(s) 170
BspLI GGNNCC 4 cut(s) 24, 356, 677, 885
BspMAI CTGCAG 2 cut(s) 247, 516
BspPI GGATC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 817
BsrGI TGTACA 2 cut(s) 1012, 1026
BsrI ACTGG 5 cut(s) 168, 323, 680, 887, 964
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 2 cut(s) 250, 748
BssNI GRCGYC 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 57, 756
BstACI GRCGYC 1 cut(s) 399
BstAPI GCANNNNNTGC 1 cut(s) 536
BstAUI TGTACA 2 cut(s) 1012, 1026
BstBAI YACGTR 1 cut(s) 793
BstC8I GCNNGC 2 cut(s) 103, 820
BstDEI CTNAG 2 cut(s) 231, 944
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 2 cut(s) 481, 625
BstKTI GATC 2 cut(s) 253, 751
BstMBI GATC 2 cut(s) 250, 748
BstMWI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 822
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 2 cut(s) 133, 280
BstSFI CTRYAG 2 cut(s) 243, 512
BstSNI TACGTA 1 cut(s) 793
BstV1I GCAGC 2 cut(s) 85, 217
BstV2I GAAGAC 1 cut(s) 41
BstXI CCANNNNNNTGG 1 cut(s) 387
Bsu15I ATCGAT 1 cut(s) 834
BsuRI GGCC 1 cut(s) 279
BsuTUI ATCGAT 1 cut(s) 834
BtrI CACGTC 1 cut(s) 877
BtsCI GGATG 2 cut(s) 481, 625
BtsI GCAGTG 2 cut(s) 787, 903
BtsIMutI CAGTG 4 cut(s) 161, 787, 894, 903
Cac8I GCNNGC 2 cut(s) 103, 820
Cfr13I GGNCC 3 cut(s) 278, 320, 962
ClaI ATCGAT 1 cut(s) 834
CseI GACGC 1 cut(s) 824
Csp6I GTAC 3 cut(s) 23, 1013, 1027
CviAII CATG 3 cut(s) 406, 819, 1055
CviQI GTAC 3 cut(s) 23, 1013, 1027
DdeI CTNAG 2 cut(s) 231, 944
DpnI GATC 2 cut(s) 252, 750
DpnII GATC 2 cut(s) 250, 748
Ecl136II GAGCTC 1 cut(s) 239
Eco105I TACGTA 1 cut(s) 793
Eco24I GRGCYC 1 cut(s) 241
Eco32I GATATC 2 cut(s) 832, 917
Eco47I GGWCC 2 cut(s) 320, 962
Eco53kI GAGCTC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 1 cut(s) 280
EcoRV GATATC 2 cut(s) 832, 917
EcoT22I ATGCAT 1 cut(s) 824
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 3 cut(s) 409, 822, 1058
FalI AAGNNNNNCTT 2 cut(s) 703, 735
FatI CATG 3 cut(s) 405, 818, 1054
Fnu4HI GCNGC 3 cut(s) 99, 206, 765
FokI GGATG 2 cut(s) 488, 632
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 3 cut(s) 99, 206, 765
FspBI CTAG 1 cut(s) 998
GluI GCNGC 3 cut(s) 99, 206, 765
GsuI CTGGAG 1 cut(s) 545
HaeIII GGCC 1 cut(s) 279
HapII CCGG 3 cut(s) 134, 144, 171
HgaI GACGC 1 cut(s) 824
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 3 cut(s) 409, 822, 1058
HinfI GANTC 9 cut(s) 127, 167, 227, 303, 438, 572, 652, 689, 803
HpaII CCGG 3 cut(s) 134, 144, 171
HphI GGTGA 3 cut(s) 158, 200, 581
Hpy166II GTNNAC 3 cut(s) 796, 941, 1027
Hpy188I TCNGA 4 cut(s) 232, 748, 829, 934
Hpy188III TCNNGA 3 cut(s) 171, 371, 673
Hpy8I GTNNAC 3 cut(s) 796, 941, 1027
HpyAV CCTTC 4 cut(s) 161, 586, 893, 929
HpyCH4III ACNGT 2 cut(s) 57, 756
HpyCH4IV ACGT 6 cut(s) 216, 399, 792, 798, 861, 876
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
HpyF3I CTNAG 2 cut(s) 231, 944
HpySE526I ACGT 6 cut(s) 216, 399, 792, 798, 861, 876
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 3 cut(s) 409, 822, 1058
Kpn2I TCCGGA 1 cut(s) 170
KpnI GGTACC 1 cut(s) 26
Kzo9I GATC 2 cut(s) 250, 748
LmnI GCTCC 3 cut(s) 244, 354, 1039
Lsp1109I GCAGC 2 cut(s) 85, 217
LweI GCATC 4 cut(s) 409, 691, 826, 835
MaeI CTAG 1 cut(s) 998
MaeII ACGT 6 cut(s) 216, 399, 792, 798, 861, 876
MaeIII GTNAC 5 cut(s) 146, 188, 325, 569, 682
MalI GATC 2 cut(s) 252, 750
MboI GATC 2 cut(s) 250, 748
MboII GAAGA 5 cut(s) 5, 41, 209, 298, 924
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 4 cut(s) 343, 506, 588, 714
MlyI GAGTC 2 cut(s) 236, 566
MnlI CCTC 6 cut(s) 264, 269, 520, 654, 998, 1047
Mph1103I ATGCAT 1 cut(s) 824
MroI TCCGGA 1 cut(s) 170
MseI TTAA 2 cut(s) 59, 333
MslI CAYNNNNRTG 3 cut(s) 385, 590, 973
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 3 cut(s) 134, 144, 171
MspR9I CCNGG 2 cut(s) 135, 282
Mva1269I GAATGC 1 cut(s) 1003
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
NciI CCSGG 1 cut(s) 135
NdeII GATC 2 cut(s) 250, 748
NlaIII CATG 3 cut(s) 409, 822, 1058
NlaIV GGNNCC 4 cut(s) 24, 356, 677, 885
NmuCI GTSAC 3 cut(s) 146, 188, 569
NsiI ATGCAT 1 cut(s) 824
NspI RCATGY 1 cut(s) 822
PaeI GCATGC 1 cut(s) 822
PctI GAATGC 1 cut(s) 1003
PfeI GAWTC 7 cut(s) 127, 167, 303, 438, 652, 689, 803
PkrI GCNGC 3 cut(s) 100, 207, 766
PleI GAGTC 2 cut(s) 235, 566
PpsI GAGTC 2 cut(s) 235, 566
Ppu21I YACGTR 1 cut(s) 793
Psp124BI GAGCTC 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 280
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 4 cut(s) 24, 356, 677, 885
PspPI GGNCC 3 cut(s) 278, 320, 962
PstI CTGCAG 2 cut(s) 247, 516
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 3 cut(s) 24, 1014, 1028
RsaNI GTAC 3 cut(s) 23, 1013, 1027
RseI CAYNNNNRTG 3 cut(s) 385, 590, 973
SacI GAGCTC 1 cut(s) 241
SaqAI TTAA 2 cut(s) 59, 333
SatI GCNGC 3 cut(s) 99, 206, 765
Sau3AI GATC 2 cut(s) 250, 748
Sau96I GGNCC 3 cut(s) 278, 320, 962
SchI GAGTC 2 cut(s) 236, 566
ScrFI CCNGG 2 cut(s) 135, 282
SduI GDGCHC 1 cut(s) 241
SfaNI GCATC 4 cut(s) 409, 691, 826, 835
SfcI CTRYAG 2 cut(s) 243, 512
SinI GGWCC 2 cut(s) 320, 962
SmiMI CAYNNNNRTG 3 cut(s) 385, 590, 973
SmlI CTYRAG 1 cut(s) 656
SmoI CTYRAG 1 cut(s) 656
SnaBI TACGTA 1 cut(s) 793
SphI GCATGC 1 cut(s) 822
Sse9I AATT 4 cut(s) 343, 506, 588, 714
SsiI CCGC 2 cut(s) 462, 764
SspI AATATT 1 cut(s) 615
SspMI CTAG 1 cut(s) 998
SstI GAGCTC 1 cut(s) 241
StyD4I CCNGG 2 cut(s) 133, 280
TaaI ACNGT 2 cut(s) 57, 756
TaiI ACGT 6 cut(s) 219, 402, 795, 801, 864, 879
TaqI TCGA 2 cut(s) 385, 834
TasI AATT 4 cut(s) 343, 506, 588, 714
TatI WGTACW 2 cut(s) 1012, 1026
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 7 cut(s) 127, 167, 303, 438, 652, 689, 803
Tru1I TTAA 2 cut(s) 59, 333
Tru9I TTAA 2 cut(s) 59, 333
TscAI CASTG 4 cut(s) 168, 787, 894, 910
TseFI GTSAC 3 cut(s) 146, 188, 569
TseI GCWGC 2 cut(s) 98, 205
Tsp45I GTSAC 3 cut(s) 146, 188, 569
TspDTI ATGAA 8 cut(s) 5, 47, 210, 519, 642, 665, 681, 685
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 4 cut(s) 168, 787, 894, 910
VpaK11BI GGWCC 2 cut(s) 320, 962
XapI RAATTY 2 cut(s) 506, 714
XceI RCATGY 1 cut(s) 822
XspI CTAG 1 cut(s) 998
ZraI GACGTC 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.