Rroxscaffold_5G00343280

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000839
Physical Location & Seq
Forward (+)
12571279 .. 12577494
6216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343280.1

Sequence Viewer

Length: 1299 bp
ATGCCTACCATGACGAAATTGATGGGTTTGCGACTGCTTCTTGTATTTGCTTTCACTCTCATACTCGCTTCAACTTCTTCGGCAAATGTCACTACCACCACAATTACCACTCTACCTGGTTACTCTGGCGACCTCCCTTTCTCTTTCGAAACTGGGTACGTTGGTGTAGGTGACAATGAAGAAGTGCAGCTATTTTACTACTTTGTCGAGTCTCAGAGGACTCCAGCACAGGACCCTCTGCTGTTGTGGATCCCCGCAGGACCTGGTTGCTCTGGTCAGATTGTACTCTTCTTTGAGAGTGGTCCACTACTTTTCGATTATGGTGAGTACGATGGGAGCCTACCAACACTTCACGACAATCCTTTCACATGGACAGAGAGCCTCAACCTATTATACGTGGATGCACCTGTTGGTGCTGGATACTCCTACTCAACAACCCAAAGTGGTTATGTAATGGACGACTATAAACATGTGGCACAACTATATGAATTTCTCCAGAAGTGGTTAGATGCACACCCTACATACCTGAAAAACATACTCTACATTGGTGGTGACTCCTATTCTGGAATGCCTGTTCCCATGCTTGCACAAACAATAATCAATGGTAATGAAAATGGAAGTATCCCCATGATGAATTTCAGAGGATATGTGATTGGGAACCCAGTAACTGATACAGCGGTCGATGGTAATGCACAAATTCCTATTGCTCATCGGCTATCTCTTATATCGGATCAACTATATAAGTCACTTAAAACAAGTTGCCATGGGGAATATGTAACTGTAAATTCCAGCAATCATGCATGCATTGCAGATCTTGATGCCTTTGATGCACTAACTAGTGACATAAATACATTACATATCTTGGAACCATTATGTAGTAATGGTTTACCTACACAAAAGGAAATGGTTTTGACTCGGAGATATCTCAAGGAAACATCTAGCGGTTTACAAAGATCACGACCTAACGGCCCTGCACTTTGGTGTCGGAGTTATCGAAGCATGCTCAACACCGTTTGGGCTAACAACAAAGGTGTTCAAGATGCTCTTGGTGTCCGAAGGGGCACGAAAAGGCTTTGGCAACACTGTAATACGACATTGGACTACACTGAAGATGTCACCAGTGTAATCATTTATCACCAAAGTCTTTCAAAAATGGCTGATCTACGTGTTCTGATATACAGTGGTGATCATGACATGCAAGTTCCTCATATTGGTACACAAAGATGGATAAGCACACTGAATTTGACTGAGGATGAATCCTGGAGGACATGGTCAGTTGATGGCCAAACTGCAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

432

Amino Acids

48.06

Weight (kDa)

5.08

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 39 - 432 1.6e-98 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 255, 677, 942
AclWI GGATC 3 cut(s) 244, 257, 738
AcoI YGGCCR 1 cut(s) 1282
AcsI RAATTY 5 cut(s) 488, 634, 696, 784, 1240
AcuI CTGAAG 1 cut(s) 1128
AfaI GTAC 4 cut(s) 158, 285, 329, 1216
AfiI CCNNNNNNNGG 1 cut(s) 1211
AflIII ACRYGT 2 cut(s) 469, 1165
AgsI TTSAA 3 cut(s) 72, 1037, 1149
AhlI ACTAGT 1 cut(s) 836
AjnI CCWGG 3 cut(s) 115, 262, 1259
AleI CACNNNNGTG 1 cut(s) 979
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw26I GTCTC 1 cut(s) 216
AlwI GGATC 3 cut(s) 244, 257, 738
AlwNI CAGNNNCTG 2 cut(s) 263, 668
AoxI GGCC 2 cut(s) 967, 1282
ApeKI GCWGC 1 cut(s) 187
ApoI RAATTY 5 cut(s) 488, 634, 696, 784, 1240
AspS9I GGNCC 4 cut(s) 232, 260, 302, 968
AsuHPI GGTGA 6 cut(s) 182, 335, 563, 1108, 1127, 1196
AsuII TTCGAA 1 cut(s) 147
AvaII GGWCC 3 cut(s) 232, 260, 302
BaeGI GKGCMC 1 cut(s) 1064
BaeI ACNNNNGTAYC 4 cut(s) 148, 181, 663, 696
BalI TGGCCA 1 cut(s) 1284
BamHI GGATCC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 199
BccI CCATC 5 cut(s) 16, 326, 677, 1218, 1274
BceAI ACGGC 1 cut(s) 982
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 3 cut(s) 117, 264, 1261
BciVI GTATCC 2 cut(s) 413, 632
BclI TGATCA 1 cut(s) 1186
BcoDI GTCTC 1 cut(s) 216
BcuI ACTAGT 1 cut(s) 836
BfaI CTAG 2 cut(s) 837, 939
BfmI CTRYAG 1 cut(s) 1290
BfuI GTATCC 2 cut(s) 413, 632
BglII AGATCT 1 cut(s) 811
BisI GCNGC 1 cut(s) 188
BlsI GCNGC 1 cut(s) 189
Bme1390I CCNGG 3 cut(s) 117, 264, 1261
Bme18I GGWCC 3 cut(s) 232, 260, 302
BmgT120I GGNCC 4 cut(s) 232, 260, 302, 968
BmiI GGNNCC 5 cut(s) 234, 251, 338, 659, 867
BmrFI CCNGG 3 cut(s) 117, 264, 1261
BmrI ACTGGG 2 cut(s) 162, 656
BmsI GCATC 5 cut(s) 391, 499, 808, 817, 1030
BmuI ACTGGG 2 cut(s) 162, 656
BpmI CTGGAG 3 cut(s) 207, 479, 1282
Bpu14I TTCGAA 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 911
BsaAI YACGTR 2 cut(s) 397, 1166
BsaJI CCNNGG 1 cut(s) 763
Bsc4I CCNNNNNNNGG 1 cut(s) 1211
Bse1I ACTGG 3 cut(s) 157, 662, 1119
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 3 cut(s) 117, 264, 1261
BseDI CCNNGG 1 cut(s) 763
BseGI GGATG 2 cut(s) 406, 1258
BseLI CCNNNNNNNGG 1 cut(s) 1211
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 2 cut(s) 227, 1239
BseNI ACTGG 3 cut(s) 157, 662, 1119
BseSI GKGCMC 1 cut(s) 1064
BseXI GCAGC 1 cut(s) 199
BsgI GTGCAG 2 cut(s) 206, 957
Bsh1285I CGRYCG 1 cut(s) 681
BshFI GGCC 2 cut(s) 969, 1284
BsiEI CGRYCG 1 cut(s) 681
BslI CCNNNNNNNGG 1 cut(s) 1211
BsmAI GTCTC 1 cut(s) 216
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 2 cut(s) 969, 1284
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 1064
Bsp143I GATC 6 cut(s) 249, 730, 811, 953, 1159, 1186
Bsp19I CCATGG 1 cut(s) 763
BspACI CCGC 3 cut(s) 255, 677, 942
BspANI GGCC 2 cut(s) 969, 1284
BspCNI CTCAG 2 cut(s) 226, 1240
BspHI TCATGA 1 cut(s) 1189
BspLI GGNNCC 5 cut(s) 234, 251, 338, 659, 867
BspMAI CTGCAG 1 cut(s) 1294
BspPI GGATC 3 cut(s) 244, 257, 738
BspT104I TTCGAA 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 804
BsrI ACTGG 3 cut(s) 157, 662, 1119
BssECI CCNNGG 1 cut(s) 763
BssMI GATC 6 cut(s) 249, 730, 811, 953, 1159, 1186
BssT1I CCWWGG 1 cut(s) 763
Bst2UI CCWGG 3 cut(s) 117, 264, 1261
Bst4CI ACNGT 4 cut(s) 781, 1012, 1085, 1181
Bst6I CTCTTC 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 806
BstBAI YACGTR 2 cut(s) 397, 1166
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 3 cut(s) 585, 802, 1001
BstDEI CTNAG 2 cut(s) 213, 1248
BstDSI CCRYGG 1 cut(s) 763
BstF5I GGATG 2 cut(s) 406, 1258
BstKTI GATC 6 cut(s) 252, 733, 814, 956, 1162, 1189
BstMAI GTCTC 1 cut(s) 216
BstMBI GATC 6 cut(s) 249, 730, 811, 953, 1159, 1186
BstMCI CGRYCG 1 cut(s) 681
BstMWI GCNNNNNNNGC 2 cut(s) 806, 827
BstNI CCWGG 3 cut(s) 117, 264, 1261
BstNSI RCATGY 4 cut(s) 473, 804, 1003, 1198
BstSCI CCNGG 3 cut(s) 115, 262, 1259
BstSFI CTRYAG 1 cut(s) 1290
BstSLI GKGCMC 1 cut(s) 1064
BstV1I GCAGC 1 cut(s) 199
BstX2I RGATCY 2 cut(s) 249, 811
BstYI RGATCY 2 cut(s) 249, 811
BsuI GTATCC 2 cut(s) 413, 632
BsuRI GGCC 2 cut(s) 969, 1284
BtgI CCRYGG 1 cut(s) 763
BtsCI GGATG 2 cut(s) 406, 1258
BtsIMutI CAGTG 5 cut(s) 1081, 1104, 1126, 1186, 1235
Cac8I GCNNGC 3 cut(s) 585, 802, 1001
CaiI CAGNNNCTG 2 cut(s) 263, 668
CciI TCATGA 1 cut(s) 1189
Cfr13I GGNCC 4 cut(s) 232, 260, 302, 968
CsiI ACCWGGT 2 cut(s) 115, 262
Csp6I GTAC 4 cut(s) 157, 284, 328, 1215
CviJI RGCY 9 cut(s) 190, 339, 381, 715, 969, 1019, 1072, 1157, 1284
CviKI_1 RGCY 9 cut(s) 190, 339, 381, 715, 969, 1019, 1072, 1157, 1284
CviQI GTAC 4 cut(s) 157, 284, 328, 1215
DdeI CTNAG 2 cut(s) 213, 1248
DpnI GATC 6 cut(s) 251, 732, 813, 955, 1161, 1188
DpnII GATC 6 cut(s) 249, 730, 811, 953, 1159, 1186
EaeI YGGCCR 1 cut(s) 1282
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 763
Eco32I GATATC 1 cut(s) 923
Eco47I GGWCC 3 cut(s) 232, 260, 302
Eco57I CTGAAG 1 cut(s) 1128
EcoO109I RGGNCCY 2 cut(s) 232, 260
EcoRII CCWGG 3 cut(s) 115, 262, 1259
EcoRV GATATC 1 cut(s) 923
EcoT14I CCWWGG 1 cut(s) 763
EcoT22I ATGCAT 2 cut(s) 802, 806
ErhI CCWWGG 1 cut(s) 763
FalI AAGNNNNNCTT 2 cut(s) 1029, 1061
FauI CCCGC 1 cut(s) 262
FbaI TGATCA 1 cut(s) 1186
Fnu4HI GCNGC 1 cut(s) 188
FokI GGATG 2 cut(s) 413, 1265
Fsp4HI GCNGC 1 cut(s) 188
FspBI CTAG 2 cut(s) 837, 939
GluI GCNGC 1 cut(s) 188
GsuI CTGGAG 3 cut(s) 207, 479, 1282
HaeIII GGCC 2 cut(s) 969, 1284
HinfI GANTC 5 cut(s) 209, 220, 554, 913, 1256
HphI GGTGA 6 cut(s) 182, 335, 563, 1108, 1127, 1196
Hpy166II GTNNAC 4 cut(s) 305, 887, 947, 1217
Hpy188I TCNGA 8 cut(s) 216, 279, 641, 730, 918, 987, 1055, 1173
Hpy188III TCNNGA 7 cut(s) 353, 496, 564, 815, 957, 1037, 1190
Hpy8I GTNNAC 4 cut(s) 305, 887, 947, 1217
HpyAV CCTTC 1 cut(s) 1050
HpyCH4III ACNGT 4 cut(s) 781, 1012, 1085, 1181
HpyCH4IV ACGT 3 cut(s) 159, 396, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 806, 827
HpyF3I CTNAG 2 cut(s) 213, 1248
HpySE526I ACGT 3 cut(s) 159, 396, 1165
Ksp22I TGATCA 1 cut(s) 1186
Kzo9I GATC 6 cut(s) 249, 730, 811, 953, 1159, 1186
LmnI GCTCC 1 cut(s) 336
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 5 cut(s) 391, 499, 808, 817, 1030
MabI ACCWGGT 2 cut(s) 115, 262
MaeI CTAG 2 cut(s) 837, 939
MaeII ACGT 3 cut(s) 159, 396, 1165
MaeIII GTNAC 9 cut(s) 88, 119, 170, 551, 664, 744, 775, 839, 1114
MalI GATC 6 cut(s) 251, 732, 813, 955, 1161, 1188
MboI GATC 6 cut(s) 249, 730, 811, 953, 1159, 1186
MboII GAAGA 4 cut(s) 69, 191, 280, 1121
MflI RGATCY 2 cut(s) 249, 811
MhlI GDGCHC 1 cut(s) 1064
MlsI TGGCCA 1 cut(s) 1284
MluCI AATT 7 cut(s) 17, 102, 488, 634, 696, 784, 1240
MluNI TGGCCA 1 cut(s) 1284
MlyI GAGTC 4 cut(s) 214, 218, 548, 907
MmeI TCCRAC 1 cut(s) 965
MnlI CCTC 8 cut(s) 143, 210, 246, 392, 635, 1215, 1243, 1257
Mox20I TGGCCA 1 cut(s) 1284
Mph1103I ATGCAT 2 cut(s) 802, 806
MscI TGGCCA 1 cut(s) 1284
MseI TTAA 1 cut(s) 750
MslI CAYNNNNRTG 2 cut(s) 979, 1222
Msp20I TGGCCA 1 cut(s) 1284
MspA1I CMGCKG 1 cut(s) 677
MspR9I CCNGG 3 cut(s) 117, 264, 1261
Mva1269I GAATGC 1 cut(s) 573
MvaI CCWGG 3 cut(s) 117, 264, 1261
MwoI GCNNNNNNNGC 2 cut(s) 806, 827
NcoI CCATGG 1 cut(s) 763
NdeII GATC 6 cut(s) 249, 730, 811, 953, 1159, 1186
NlaIV GGNNCC 5 cut(s) 234, 251, 338, 659, 867
NmuCI GTSAC 6 cut(s) 88, 170, 551, 744, 839, 1114
NsiI ATGCAT 2 cut(s) 802, 806
NspI RCATGY 4 cut(s) 473, 804, 1003, 1198
NspV TTCGAA 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 979
PaeI GCATGC 2 cut(s) 804, 1003
PagI TCATGA 1 cut(s) 1189
PciI ACATGT 1 cut(s) 469
PcsI WCGNNNNNNNCGW 1 cut(s) 991
PctI GAATGC 1 cut(s) 573
PfeI GAWTC 1 cut(s) 1256
PflFI GACNNNGTC 1 cut(s) 1270
PfoI TCCNGGA 1 cut(s) 1259
PkrI GCNGC 1 cut(s) 189
PleI GAGTC 4 cut(s) 214, 217, 548, 907
PpsI GAGTC 4 cut(s) 214, 217, 548, 907
Ppu21I YACGTR 2 cut(s) 397, 1166
PpuMI RGGWCCY 2 cut(s) 232, 260
PscI ACATGT 1 cut(s) 469
Psp5II RGGWCCY 2 cut(s) 232, 260
Psp6I CCWGG 3 cut(s) 115, 262, 1259
PspGI CCWGG 3 cut(s) 115, 262, 1259
PspN4I GGNNCC 5 cut(s) 234, 251, 338, 659, 867
PspPI GGNCC 4 cut(s) 232, 260, 302, 968
PspPPI RGGWCCY 2 cut(s) 232, 260
PstI CTGCAG 1 cut(s) 1294
PstNI CAGNNNCTG 2 cut(s) 263, 668
PsuI RGATCY 2 cut(s) 249, 811
PsyI GACNNNGTC 1 cut(s) 1270
RsaI GTAC 4 cut(s) 158, 285, 329, 1216
RsaNI GTAC 4 cut(s) 157, 284, 328, 1215
RseI CAYNNNNRTG 2 cut(s) 979, 1222
SaqAI TTAA 1 cut(s) 750
SatI GCNGC 1 cut(s) 188
Sau3AI GATC 6 cut(s) 249, 730, 811, 953, 1159, 1186
Sau96I GGNCC 4 cut(s) 232, 260, 302, 968
SchI GAGTC 4 cut(s) 214, 218, 548, 907
ScrFI CCNGG 3 cut(s) 117, 264, 1261
SduI GDGCHC 1 cut(s) 1064
SexAI ACCWGGT 2 cut(s) 115, 262
SfaNI GCATC 5 cut(s) 391, 499, 808, 817, 1030
SfcI CTRYAG 1 cut(s) 1290
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 3 cut(s) 232, 260, 302
SmiMI CAYNNNNRTG 2 cut(s) 979, 1222
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
SpeI ACTAGT 1 cut(s) 836
SphI GCATGC 2 cut(s) 804, 1003
Sse9I AATT 7 cut(s) 17, 102, 488, 634, 696, 784, 1240
SsiI CCGC 3 cut(s) 255, 677, 942
SspMI CTAG 2 cut(s) 837, 939
StyD4I CCNGG 3 cut(s) 115, 262, 1259
StyI CCWWGG 1 cut(s) 763
TaaI ACNGT 4 cut(s) 781, 1012, 1085, 1181
TaiI ACGT 3 cut(s) 162, 399, 1168
TaqI TCGA 5 cut(s) 147, 207, 315, 681, 994
TasI AATT 7 cut(s) 17, 102, 488, 634, 696, 784, 1240
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 1 cut(s) 1256
Tru1I TTAA 1 cut(s) 750
Tru9I TTAA 1 cut(s) 750
TscAI CASTG 5 cut(s) 1088, 1111, 1126, 1186, 1242
TseFI GTSAC 6 cut(s) 88, 170, 551, 744, 839, 1114
TseI GCWGC 1 cut(s) 187
Tsp45I GTSAC 6 cut(s) 88, 170, 551, 744, 839, 1114
TspDTI ATGAA 5 cut(s) 192, 501, 624, 647, 1269
TspRI CASTG 5 cut(s) 1088, 1111, 1126, 1186, 1242
Tth111I GACNNNGTC 1 cut(s) 1270
VpaK11BI GGWCC 3 cut(s) 232, 260, 302
XapI RAATTY 5 cut(s) 488, 634, 696, 784, 1240
XceI RCATGY 4 cut(s) 473, 804, 1003, 1198
XspI CTAG 2 cut(s) 837, 939
Zsp2I ATGCAT 2 cut(s) 802, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.