Rh4DG077300

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000839
Physical Location & Seq
Forward (+)
11472022 .. 11476440
4419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG077300.1

Sequence Viewer

Length: 1425 bp
ATGGATTTTGTTCTTCATTTGGTACCCACAATGAAAATGATGGATTTTCTTCTATTGTTAATGTTGGCTTTCACTTCGATACTCACTTCAACTTCAGCTGCCAGCATTACTACAACCAACATTACGATTCTACCGGGTTATTCCGGTGACCTTCCTTTTGCACTGGAATCCGGATATATCGGTGTAGGTGACAATGAAGAAGTGCAGCTATTTTACGTTTTCGTTGAGTCTGAGAGAACTCCTGCGGACGATCCTCTGTTGCTATGGTTCAATGGAGGCCCAGGTTGCTCTGGTCTTGCTGGATTATTTTTTGAGAGTGGTCCAGTAACTTTTAAATTTGGAAATTACAATGGGAGCCTACCAGCAGTTCTTGACAATCCATTTCGATGGACAAAGGACGTCAGCATGATATACATAGATGCACCTGTTGGTGCTGGATTCTCCTACTCAAAAACTCAAAGCGGTTATGTTATGGATGACTATAAATATGTTGCACAAAGCTATGAATTTCTGCAGAAGTGGCTGGAGGCACACCCTGCATATTTGGAAAATATGCTCTACATTGGTGGTGACTCCTTCTCTGGCATAATTGTGCCAATGATTGTGCAACAAATATTGGATGGTAATGAAAATGGAACTTTGCCTCTAATGAATCTCAAGGGATATGTAATTGGGAACCCAGTAACGGATTCATTCATTGATGCAAATGCAAGAATTCCTTATGCCCATCGGCTAACTCTTATATCAGATCAACTGTATCAGGCGGCGAAAACAAGTTGCAGTGGAGATTACGTAAACGTAGATTCAAGCAATGACGCATGCATTTCAGATGTCGATGCTATTGATGCACTCGTAAATGACGTAAATACCTTACACGTCTTGGAACCACTGTGTACTGTAGGCACTGCCCGAAGATATCTCAAAGATTTGTCCGAAGGTTTACTAAGTTCAGCGACTACTGGTCCTGCATATTGGTGTAGGAGTTATAATCATATGCTCGCCTACAATTGGGCAAACCACCAAAGTGTTCAAGATGCTCTTGGCGTCCGAAGGGGCACAAAAAGATTTTGGCAATACTGTAATTCAACCCTGGCGTACACTCAAGAAGTCACTAGTGTTGTTAGTCTTCATCAAAGTTTCACGGAGGTTGGCCTACGTGCTCTGATCTACAGTGGTGATCACGACATGACAATTCCTCACATTGCTACACAAAAATGGATAAACTTTCTCAATATGACTCTGAATGAGACCTGGAGGGCATGGTCAGTTGATAACCAAACTGCAGGGTACACAAAGAAGTTTGTAAATGAAGCTTTCAATTTGACGTTTGCGACTATAAAGGGAGGAGGCCACATTGCTGCAGAGTACAAGGTCAAAGAATGCTCTGCAATGATTGATAGATGGATGGCTCAGTACCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

474

Amino Acids

52.66

Weight (kDa)

4.87

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 45 - 470 1.9e-116 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 987
AatII GACGTC 1 cut(s) 402
Acc65I GGTACC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 3 cut(s) 245, 462, 764
AclWI GGATC 1 cut(s) 245
AcsI RAATTY 3 cut(s) 335, 506, 714
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 399, 1044
AfaI GTAC 6 cut(s) 24, 895, 1097, 1289, 1367, 1415
AfiI CCNNNNNNNGG 2 cut(s) 685, 1008
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 6 cut(s) 90, 271, 807, 1031, 1086, 1318
AhdI GACNNNNNGTC 1 cut(s) 960
AhlI ACTAGT 1 cut(s) 1112
AjiI CACGTC 1 cut(s) 877
AjnI CCWGG 3 cut(s) 280, 1089, 1250
AleI CACNNNNGTG 1 cut(s) 1023
AluBI AGCT 4 cut(s) 98, 208, 501, 1313
AluI AGCT 4 cut(s) 98, 208, 501, 1313
Alw21I GWGCWC 1 cut(s) 1162
Alw26I GTCTC 1 cut(s) 1241
AlwI GGATC 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 3 cut(s) 277, 1150, 1348
ApeKI GCWGC 3 cut(s) 98, 205, 1358
ApoI RAATTY 3 cut(s) 335, 506, 714
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
Asp718I GGTACC 1 cut(s) 22
AspS9I GGNCC 3 cut(s) 278, 320, 962
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 4 cut(s) 158, 200, 581, 1187
AvaII GGWCC 2 cut(s) 320, 962
BaeGI GKGCMC 1 cut(s) 1058
BanI GGYRCC 1 cut(s) 22
BbsI GAAGAC 1 cut(s) 1118
Bbv12I GWGCWC 1 cut(s) 1162
BbvI GCAGC 3 cut(s) 85, 217, 1345
BccI CCATC 6 cut(s) 34, 381, 614, 735, 1395, 1399
BciT130I CCWGG 3 cut(s) 282, 1091, 1252
BclI TGATCA 1 cut(s) 1177
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 1241
BcuI ACTAGT 1 cut(s) 1112
BfaI CTAG 1 cut(s) 1113
BfmI CTRYAG 5 cut(s) 512, 897, 1168, 1281, 1359
BisI GCNGC 4 cut(s) 99, 206, 765, 1359
BlsI GCNGC 4 cut(s) 100, 207, 766, 1360
Bme1390I CCNGG 4 cut(s) 135, 282, 1091, 1252
Bme18I GGWCC 2 cut(s) 320, 962
BmeRI GACNNNNNGTC 1 cut(s) 960
BmgBI CACGTC 1 cut(s) 877
BmgT120I GGNCC 3 cut(s) 278, 320, 962
BmiI GGNNCC 4 cut(s) 24, 356, 677, 885
BmrFI CCNGG 4 cut(s) 135, 282, 1091, 1252
BmrI ACTGGG 1 cut(s) 674
BmsI GCATC 5 cut(s) 409, 691, 826, 835, 1024
BmuI ACTGGG 1 cut(s) 674
BpiI GAAGAC 1 cut(s) 1118
BpmI CTGGAG 2 cut(s) 545, 1273
BpuEI CTTGAG 2 cut(s) 641, 1086
BpuMI CCSGG 1 cut(s) 135
BsaAI YACGTR 2 cut(s) 793, 1157
BsaHI GRCGYC 2 cut(s) 399, 1044
BsaI GGTCTC 1 cut(s) 1241
BsaJI CCNNGG 2 cut(s) 280, 1089
BsaWI WCCGGW 2 cut(s) 143, 170
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 2 cut(s) 685, 1008
Bse1I ACTGG 4 cut(s) 168, 323, 680, 964
Bse3DI GCAATG 4 cut(s) 817, 1200, 1353, 1395
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 3 cut(s) 282, 1091, 1252
BseDI CCNNGG 2 cut(s) 280, 1089
BseGI GGATG 3 cut(s) 481, 625, 1410
BseLI CCNNNNNNNGG 2 cut(s) 685, 1008
BseMI GCAATG 4 cut(s) 817, 1200, 1353, 1395
BseMII CTCAG 2 cut(s) 222, 1424
BseNI ACTGG 4 cut(s) 168, 323, 680, 964
BseRI GAGGAG 1 cut(s) 1359
BseSI GKGCMC 1 cut(s) 1058
BseXI GCAGC 3 cut(s) 85, 217, 1345
BsgI GTGCAG 1 cut(s) 224
BshFI GGCC 3 cut(s) 279, 1152, 1350
BshNI GGYRCC 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 1162
BsiSI CCGG 3 cut(s) 134, 144, 171
BslI CCNNNNNNNGG 2 cut(s) 685, 1008
BsmAI GTCTC 1 cut(s) 1241
BsmI GAATGC 1 cut(s) 1385
BsnI GGCC 3 cut(s) 279, 1152, 1350
Bso31I GGTCTC 1 cut(s) 1241
Bsp1286I GDGCHC 2 cut(s) 1058, 1162
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 4 cut(s) 250, 748, 1164, 1177
BspACI CCGC 3 cut(s) 245, 462, 764
BspANI GGCC 3 cut(s) 279, 1152, 1350
BspCNI CTCAG 2 cut(s) 223, 1423
BspEI TCCGGA 1 cut(s) 170
BspLI GGNNCC 4 cut(s) 24, 356, 677, 885
BspMAI CTGCAG 3 cut(s) 516, 1285, 1363
BspPI GGATC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 1241
BsrDI GCAATG 4 cut(s) 817, 1200, 1353, 1395
BsrI ACTGG 4 cut(s) 168, 323, 680, 964
BssECI CCNNGG 2 cut(s) 280, 1089
BssMI GATC 4 cut(s) 250, 748, 1164, 1177
BssNI GRCGYC 2 cut(s) 399, 1044
Bst2UI CCWGG 3 cut(s) 282, 1091, 1252
Bst4CI ACNGT 5 cut(s) 756, 891, 898, 1079, 1172
BstACI GRCGYC 2 cut(s) 399, 1044
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBAI YACGTR 2 cut(s) 793, 1157
BstC8I GCNNGC 3 cut(s) 103, 820, 999
BstDEI CTNAG 3 cut(s) 231, 944, 1410
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 3 cut(s) 481, 625, 1410
BstKTI GATC 4 cut(s) 253, 751, 1167, 1180
BstMAI GTCTC 1 cut(s) 1241
BstMBI GATC 4 cut(s) 250, 748, 1164, 1177
BstMWI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
BstNI CCWGG 3 cut(s) 282, 1091, 1252
BstNSI RCATGY 1 cut(s) 822
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 4 cut(s) 133, 280, 1089, 1250
BstSFI CTRYAG 5 cut(s) 512, 897, 1168, 1281, 1359
BstSLI GKGCMC 1 cut(s) 1058
BstSNI TACGTA 1 cut(s) 793
BstV1I GCAGC 3 cut(s) 85, 217, 1345
BstV2I GAAGAC 1 cut(s) 1118
BstXI CCANNNNNNTGG 1 cut(s) 387
BsuRI GGCC 3 cut(s) 279, 1152, 1350
BtrI CACGTC 1 cut(s) 877
BtsCI GGATG 3 cut(s) 481, 625, 1410
BtsI GCAGTG 2 cut(s) 787, 903
BtsIMutI CAGTG 5 cut(s) 161, 787, 887, 903, 1177
Cac8I GCNNGC 3 cut(s) 103, 820, 999
Cfr13I GGNCC 3 cut(s) 278, 320, 962
CseI GACGC 2 cut(s) 824, 1033
Csp6I GTAC 6 cut(s) 23, 894, 1096, 1288, 1366, 1414
CviAII CATG 4 cut(s) 406, 819, 1186, 1260
CviQI GTAC 6 cut(s) 23, 894, 1096, 1288, 1366, 1414
DdeI CTNAG 3 cut(s) 231, 944, 1410
DpnI GATC 4 cut(s) 252, 750, 1166, 1179
DpnII GATC 4 cut(s) 250, 748, 1164, 1177
DraI TTTAAA 1 cut(s) 334
DriI GACNNNNNGTC 1 cut(s) 960
Eam1105I GACNNNNNGTC 1 cut(s) 960
Eco105I TACGTA 1 cut(s) 793
Eco31I GGTCTC 1 cut(s) 1241
Eco32I GATATC 1 cut(s) 917
Eco47I GGWCC 2 cut(s) 320, 962
Eco57I CTGAAG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 3 cut(s) 280, 1089, 1250
EcoRV GATATC 1 cut(s) 917
EcoT22I ATGCAT 1 cut(s) 824
FaeI CATG 4 cut(s) 409, 822, 1189, 1263
FalI AAGNNNNNCTT 4 cut(s) 703, 735, 1023, 1055
FatI CATG 4 cut(s) 405, 818, 1185, 1259
FauNDI CATATG 1 cut(s) 993
FbaI TGATCA 1 cut(s) 1177
Fnu4HI GCNGC 4 cut(s) 99, 206, 765, 1359
FokI GGATG 3 cut(s) 488, 632, 1417
Fsp4HI GCNGC 4 cut(s) 99, 206, 765, 1359
FspBI CTAG 1 cut(s) 1113
GluI GCNGC 4 cut(s) 99, 206, 765, 1359
GsuI CTGGAG 2 cut(s) 545, 1273
HaeIII GGCC 3 cut(s) 279, 1152, 1350
HapII CCGG 3 cut(s) 134, 144, 171
HgaI GACGC 2 cut(s) 824, 1033
Hin1I GRCGYC 2 cut(s) 399, 1044
Hin1II CATG 4 cut(s) 409, 822, 1189, 1263
HindIII AAGCTT 1 cut(s) 1311
HinfI GANTC 9 cut(s) 127, 167, 227, 438, 572, 652, 689, 803, 1237
HpaII CCGG 3 cut(s) 134, 144, 171
HphI GGTGA 4 cut(s) 158, 200, 581, 1187
Hpy166II GTNNAC 5 cut(s) 796, 894, 941, 1098, 1290
Hpy188I TCNGA 7 cut(s) 232, 748, 829, 934, 1049, 1164, 1242
Hpy188III TCNNGA 5 cut(s) 171, 371, 1031, 1103, 1181
Hpy8I GTNNAC 5 cut(s) 796, 894, 941, 1098, 1290
HpyAV CCTTC 4 cut(s) 161, 586, 929, 1044
HpyCH4III ACNGT 5 cut(s) 756, 891, 898, 1079, 1172
HpyCH4IV ACGT 8 cut(s) 216, 399, 792, 798, 861, 876, 1156, 1325
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
HpyF3I CTNAG 3 cut(s) 231, 944, 1410
HpySE526I ACGT 8 cut(s) 216, 399, 792, 798, 861, 876, 1156, 1325
Hsp92I GRCGYC 2 cut(s) 399, 1044
Hsp92II CATG 4 cut(s) 409, 822, 1189, 1263
Kpn2I TCCGGA 1 cut(s) 170
KpnI GGTACC 1 cut(s) 26
Ksp22I TGATCA 1 cut(s) 1177
Kzo9I GATC 4 cut(s) 250, 748, 1164, 1177
LmnI GCTCC 1 cut(s) 354
Lsp1109I GCAGC 3 cut(s) 85, 217, 1345
LweI GCATC 5 cut(s) 409, 691, 826, 835, 1024
MaeI CTAG 1 cut(s) 1113
MaeII ACGT 8 cut(s) 216, 399, 792, 798, 861, 876, 1156, 1325
MaeIII GTNAC 6 cut(s) 146, 188, 325, 569, 682, 1108
MalI GATC 4 cut(s) 252, 750, 1166, 1179
MboI GATC 4 cut(s) 250, 748, 1164, 1177
MboII GAAGA 5 cut(s) 5, 41, 209, 924, 1118
MfeI CAATTG 1 cut(s) 1006
MhlI GDGCHC 2 cut(s) 1058, 1162
MlyI GAGTC 3 cut(s) 236, 566, 1231
MnlI CCTC 9 cut(s) 264, 269, 520, 654, 1138, 1206, 1248, 1337, 1340
Mph1103I ATGCAT 1 cut(s) 824
MroI TCCGGA 1 cut(s) 170
MseI TTAA 2 cut(s) 59, 333
MslI CAYNNNNRTG 5 cut(s) 385, 590, 973, 1023, 1213
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 3 cut(s) 134, 144, 171
MspR9I CCNGG 4 cut(s) 135, 282, 1091, 1252
MunI CAATTG 1 cut(s) 1006
Mva1269I GAATGC 1 cut(s) 1385
MvaI CCWGG 3 cut(s) 282, 1091, 1252
MwoI GCNNNNNNNGC 4 cut(s) 285, 520, 536, 845
NciI CCSGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 993
NdeII GATC 4 cut(s) 250, 748, 1164, 1177
NlaIII CATG 4 cut(s) 409, 822, 1189, 1263
NlaIV GGNNCC 4 cut(s) 24, 356, 677, 885
NmuCI GTSAC 4 cut(s) 146, 188, 569, 1108
NsiI ATGCAT 1 cut(s) 824
NspI RCATGY 1 cut(s) 822
OliI CACNNNNGTG 1 cut(s) 1023
PaeI GCATGC 1 cut(s) 822
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PctI GAATGC 1 cut(s) 1385
PfeI GAWTC 6 cut(s) 127, 167, 438, 652, 689, 803
PkrI GCNGC 4 cut(s) 100, 207, 766, 1360
PleI GAGTC 3 cut(s) 235, 566, 1231
PpsI GAGTC 3 cut(s) 235, 566, 1231
Ppu21I YACGTR 2 cut(s) 793, 1157
PsiI TTATAA 1 cut(s) 987
Psp6I CCWGG 3 cut(s) 280, 1089, 1250
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 3 cut(s) 280, 1089, 1250
PspN4I GGNNCC 4 cut(s) 24, 356, 677, 885
PspPI GGNCC 3 cut(s) 278, 320, 962
PstI CTGCAG 3 cut(s) 516, 1285, 1363
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 6 cut(s) 24, 895, 1097, 1289, 1367, 1415
RsaNI GTAC 6 cut(s) 23, 894, 1096, 1288, 1366, 1414
RseI CAYNNNNRTG 5 cut(s) 385, 590, 973, 1023, 1213
SaqAI TTAA 2 cut(s) 59, 333
SatI GCNGC 4 cut(s) 99, 206, 765, 1359
Sau3AI GATC 4 cut(s) 250, 748, 1164, 1177
Sau96I GGNCC 3 cut(s) 278, 320, 962
SchI GAGTC 3 cut(s) 236, 566, 1231
ScrFI CCNGG 4 cut(s) 135, 282, 1091, 1252
SduI GDGCHC 2 cut(s) 1058, 1162
SfaNI GCATC 5 cut(s) 409, 691, 826, 835, 1024
SfcI CTRYAG 5 cut(s) 512, 897, 1168, 1281, 1359
SinI GGWCC 2 cut(s) 320, 962
SmiMI CAYNNNNRTG 5 cut(s) 385, 590, 973, 1023, 1213
SmlI CTYRAG 2 cut(s) 656, 1101
SmoI CTYRAG 2 cut(s) 656, 1101
SnaBI TACGTA 1 cut(s) 793
SpeI ACTAGT 1 cut(s) 1112
SphI GCATGC 1 cut(s) 822
SsiI CCGC 3 cut(s) 245, 462, 764
SspI AATATT 1 cut(s) 615
SspMI CTAG 1 cut(s) 1113
StyD4I CCNGG 4 cut(s) 133, 280, 1089, 1250
TaaI ACNGT 5 cut(s) 756, 891, 898, 1079, 1172
TaiI ACGT 8 cut(s) 219, 402, 795, 801, 864, 879, 1159, 1328
TaqI TCGA 3 cut(s) 77, 385, 834
TatI WGTACW 2 cut(s) 893, 1365
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 6 cut(s) 127, 167, 438, 652, 689, 803
Tru1I TTAA 2 cut(s) 59, 333
Tru9I TTAA 2 cut(s) 59, 333
TscAI CASTG 5 cut(s) 168, 787, 894, 910, 1177
TseFI GTSAC 4 cut(s) 146, 188, 569, 1108
TseI GCWGC 3 cut(s) 98, 205, 1358
Tsp45I GTSAC 4 cut(s) 146, 188, 569, 1108
TspGWI ACGGA 2 cut(s) 701, 1157
TspRI CASTG 5 cut(s) 168, 787, 894, 910, 1177
VpaK11BI GGWCC 2 cut(s) 320, 962
XapI RAATTY 3 cut(s) 335, 506, 714
XceI RCATGY 1 cut(s) 822
XspI CTAG 1 cut(s) 1113
ZraI GACGTC 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.