Rw4G007050

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000839
Physical Location & Seq
Forward (+)
14761166 .. 14765435
4270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007050.1

Sequence Viewer

Length: 1428 bp
ATGGATTTTGTTCATCATTTGGTGGTACCCGCAATGAAAATGATGGATTTTCTTCTACTGTTAATGTTCGCTTTCACTTTGATACTCACTTCAACTTCAGCTGCCAGCATTACTACAACCAACATTACGATTCTACCGGGTTATTCCGGTGACCTTCCTTTTGCACTGGAATCCGGATATATCGGTGTTGGTGACAATGAAGAAGTGCAGCTATTTTATGTTTTCGTTGAGTCTGAGAGAGCTCCTGCAGACGATCCTCTGTTGCTATGGTTCAATGGAGGCCCAGGTTGCTCTGGTCTTGCTGGATTCTTCTTTGAGAGTGGTCCGGTAACTTTTAAGTTTGGAAATTACAATGGGAGCCTACCAGCAGTTCTTGACAATCCATTTCGATGGACAAAGGACGTCAGCATGATATACATAGATGCACCTGTTGGTGCTGGATTCTCCTACTCAAAAACTCAAAGTGGTTATGTTATGAATGACTATAAATATGTTGCACAAAGCTATGAATTTCTGCAGAAGTGGCTGGAGGCACACCCTGCATATTTGGAAAATGTACTCTACATTGGTGGTGACTCCTTCTCTGGCATTATTGTGCCTATGATTGTTCAACAAATATTGGATGGTAATGAAAATGGAACTTTGCCTCTAATGAATCTCAAGGGATATGTAATTGGGAACCCAGTAACGGATTCATTCATTGATGCAAATGCAAGAATTCCTTATGCCCATCGGCTAACTCTTATATCAGATCAACTGTATCGGGCGGCGAAAACAAGTTGCAGTGGAGATTACGTAAACGTAGATTCAAGCAATGACGCATGCATTTCAGATATCGATGCTATTGATGCACTCATAAATGACATAAATACCTTACACGTCTTGGAACCAGTGTGTAGTGAAGGCACTGCCCGAAGATATCTCAAAGATTTGTCCGAAGGTTTACTAAGTTCAGCAACTACTGGTCCTGCATATTGGTGTAGGAGTTATAATCATATGCTCGCCTACAATTGGGCAAACCACCAAAGTGTTCAAGATGCTCTTGGCGTCCGAAGGGGCACAAAAAGATTTTGGCAATACTGTAATTCAACCCTGGCGTACACTCAAGAAGTCACTAGTGTTATTAGTCTTCATCAAAGTTTCACGGAGGTTGGCCTACGTGCTCTGATCTACAGTGGTGATCACGACATGACAATTCCTCACCTTGCTACACAAAAATGGATAAACTTTCTCAATATGACTCTGAATGAGACCTGGAGGGCATGGTCAGTTGATAACCAAACTGCAGGGTACACAAAGAAGTTTGTAAATGAAGCTTTCAATTTGACGTTTGCAACTGTAAAGGGAGGAGGCCACATTGCTGCAGAGTACAAGGTCAAAGAATGCTCTGCAATGATTGATAGATGGATGGCTCAGTACCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

475

Amino Acids

52.85

Weight (kDa)

5.0

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 46 - 471 1.7e-116 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 990
AatII GACGTC 1 cut(s) 405
Acc65I GGTACC 1 cut(s) 25
AccB1I GGYRCC 1 cut(s) 25
AccIII TCCGGA 1 cut(s) 173
AciI CCGC 2 cut(s) 30, 767
AclWI GGATC 1 cut(s) 248
AcsI RAATTY 2 cut(s) 509, 717
AcuI CTGAAG 1 cut(s) 81
AcyI GRCGYC 2 cut(s) 402, 1047
AfaI GTAC 6 cut(s) 27, 558, 1100, 1292, 1370, 1418
AfiI CCNNNNNNNGG 2 cut(s) 688, 1011
AflIII ACRYGT 1 cut(s) 877
AgsI TTSAA 7 cut(s) 93, 274, 611, 810, 1034, 1089, 1321
AhlI ACTAGT 1 cut(s) 1115
AjiI CACGTC 1 cut(s) 880
AjnI CCWGG 3 cut(s) 283, 1092, 1253
AleI CACNNNNGTG 1 cut(s) 1026
AluBI AGCT 5 cut(s) 101, 211, 242, 504, 1316
AluI AGCT 5 cut(s) 101, 211, 242, 504, 1316
Alw21I GWGCWC 2 cut(s) 244, 1165
Alw26I GTCTC 1 cut(s) 1244
AlwI GGATC 1 cut(s) 248
Aor13HI TCCGGA 1 cut(s) 173
AoxI GGCC 3 cut(s) 280, 1153, 1351
ApeKI GCWGC 3 cut(s) 101, 208, 1361
ApoI RAATTY 2 cut(s) 509, 717
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
Asp718I GGTACC 1 cut(s) 25
AspS9I GGNCC 3 cut(s) 281, 323, 965
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 5 cut(s) 161, 203, 584, 1190, 1193
AvaII GGWCC 2 cut(s) 323, 965
BaeGI GKGCMC 1 cut(s) 1061
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 1121
Bbv12I GWGCWC 2 cut(s) 244, 1165
BbvI GCAGC 3 cut(s) 88, 220, 1348
BccI CCATC 6 cut(s) 37, 384, 617, 738, 1398, 1402
BciT130I CCWGG 3 cut(s) 285, 1094, 1255
BclI TGATCA 1 cut(s) 1180
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 1244
BcuI ACTAGT 1 cut(s) 1115
BfaI CTAG 1 cut(s) 1116
BfmI CTRYAG 5 cut(s) 246, 515, 1171, 1284, 1362
BisI GCNGC 4 cut(s) 102, 209, 768, 1362
BlsI GCNGC 4 cut(s) 103, 210, 769, 1363
Bme1390I CCNGG 4 cut(s) 138, 285, 1094, 1255
Bme18I GGWCC 2 cut(s) 323, 965
BmgBI CACGTC 1 cut(s) 880
BmgT120I GGNCC 3 cut(s) 281, 323, 965
BmiI GGNNCC 4 cut(s) 27, 359, 680, 888
BmrFI CCNGG 4 cut(s) 138, 285, 1094, 1255
BmrI ACTGGG 1 cut(s) 677
BmsI GCATC 5 cut(s) 412, 694, 829, 838, 1027
BmuI ACTGGG 1 cut(s) 677
BpiI GAAGAC 1 cut(s) 1121
BpmI CTGGAG 2 cut(s) 548, 1276
BpuEI CTTGAG 2 cut(s) 644, 1089
BpuMI CCSGG 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 837
BsaAI YACGTR 2 cut(s) 796, 1160
BsaHI GRCGYC 2 cut(s) 402, 1047
BsaI GGTCTC 1 cut(s) 1244
BsaJI CCNNGG 2 cut(s) 283, 1092
BsaWI WCCGGW 3 cut(s) 146, 173, 325
BsaXI ACNNNNNCTCC 2 cut(s) 780, 810
Bsc4I CCNNNNNNNGG 2 cut(s) 688, 1011
Bse1I ACTGG 4 cut(s) 171, 683, 890, 967
Bse3DI GCAATG 4 cut(s) 39, 820, 1356, 1398
BseAI TCCGGA 1 cut(s) 173
BseBI CCWGG 3 cut(s) 285, 1094, 1255
BseCI ATCGAT 1 cut(s) 837
BseDI CCNNGG 2 cut(s) 283, 1092
BseGI GGATG 2 cut(s) 628, 1413
BseLI CCNNNNNNNGG 2 cut(s) 688, 1011
BseMI GCAATG 4 cut(s) 39, 820, 1356, 1398
BseMII CTCAG 2 cut(s) 225, 1427
BseNI ACTGG 4 cut(s) 171, 683, 890, 967
BseRI GAGGAG 1 cut(s) 1362
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 3 cut(s) 88, 220, 1348
BsgI GTGCAG 1 cut(s) 227
BshFI GGCC 3 cut(s) 282, 1155, 1353
BshNI GGYRCC 1 cut(s) 25
BshVI ATCGAT 1 cut(s) 837
BsiHKAI GWGCWC 2 cut(s) 244, 1165
BsiSI CCGG 4 cut(s) 137, 147, 174, 326
BslI CCNNNNNNNGG 2 cut(s) 688, 1011
BsmAI GTCTC 1 cut(s) 1244
BsmI GAATGC 1 cut(s) 1388
BsnI GGCC 3 cut(s) 282, 1155, 1353
Bso31I GGTCTC 1 cut(s) 1244
Bsp1286I GDGCHC 3 cut(s) 244, 1061, 1165
Bsp13I TCCGGA 1 cut(s) 173
Bsp143I GATC 4 cut(s) 253, 751, 1167, 1180
BspACI CCGC 2 cut(s) 30, 767
BspANI GGCC 3 cut(s) 282, 1155, 1353
BspCNI CTCAG 2 cut(s) 226, 1426
BspDI ATCGAT 1 cut(s) 837
BspEI TCCGGA 1 cut(s) 173
BspLI GGNNCC 4 cut(s) 27, 359, 680, 888
BspMAI CTGCAG 4 cut(s) 250, 519, 1288, 1366
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 25
BspTNI GGTCTC 1 cut(s) 1244
BsrDI GCAATG 4 cut(s) 39, 820, 1356, 1398
BsrI ACTGG 4 cut(s) 171, 683, 890, 967
BssECI CCNNGG 2 cut(s) 283, 1092
BssMI GATC 4 cut(s) 253, 751, 1167, 1180
BssNI GRCGYC 2 cut(s) 402, 1047
Bst2UI CCWGG 3 cut(s) 285, 1094, 1255
Bst4CI ACNGT 5 cut(s) 60, 759, 1082, 1175, 1339
BstACI GRCGYC 2 cut(s) 402, 1047
BstAPI GCANNNNNTGC 1 cut(s) 539
BstBAI YACGTR 2 cut(s) 796, 1160
BstC8I GCNNGC 3 cut(s) 106, 823, 1002
BstDEI CTNAG 3 cut(s) 234, 947, 1413
BstEII GGTNACC 1 cut(s) 149
BstF5I GGATG 2 cut(s) 628, 1413
BstKTI GATC 4 cut(s) 256, 754, 1170, 1183
BstMAI GTCTC 1 cut(s) 1244
BstMBI GATC 4 cut(s) 253, 751, 1167, 1180
BstMWI GCNNNNNNNGC 4 cut(s) 288, 523, 539, 848
BstNI CCWGG 3 cut(s) 285, 1094, 1255
BstNSI RCATGY 1 cut(s) 825
BstPI GGTNACC 1 cut(s) 149
BstSCI CCNGG 4 cut(s) 136, 283, 1092, 1253
BstSFI CTRYAG 5 cut(s) 246, 515, 1171, 1284, 1362
BstSLI GKGCMC 1 cut(s) 1061
BstSNI TACGTA 1 cut(s) 796
BstV1I GCAGC 3 cut(s) 88, 220, 1348
BstV2I GAAGAC 1 cut(s) 1121
BstXI CCANNNNNNTGG 1 cut(s) 390
Bsu15I ATCGAT 1 cut(s) 837
BsuRI GGCC 3 cut(s) 282, 1155, 1353
BsuTUI ATCGAT 1 cut(s) 837
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 2 cut(s) 628, 1413
BtsI GCAGTG 2 cut(s) 790, 906
BtsIMutI CAGTG 5 cut(s) 164, 790, 897, 906, 1180
Cac8I GCNNGC 3 cut(s) 106, 823, 1002
Cfr13I GGNCC 3 cut(s) 281, 323, 965
ClaI ATCGAT 1 cut(s) 837
CseI GACGC 2 cut(s) 827, 1036
Csp6I GTAC 6 cut(s) 26, 557, 1099, 1291, 1369, 1417
CviAII CATG 4 cut(s) 409, 822, 1189, 1263
CviQI GTAC 6 cut(s) 26, 557, 1099, 1291, 1369, 1417
DdeI CTNAG 3 cut(s) 234, 947, 1413
DpnI GATC 4 cut(s) 255, 753, 1169, 1182
DpnII GATC 4 cut(s) 253, 751, 1167, 1180
Ecl136II GAGCTC 1 cut(s) 242
Eco105I TACGTA 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 244
Eco31I GGTCTC 1 cut(s) 1244
Eco32I GATATC 2 cut(s) 835, 920
Eco47I GGWCC 2 cut(s) 323, 965
Eco53kI GAGCTC 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 81
Eco91I GGTNACC 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 242
EcoO65I GGTNACC 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 717
EcoRII CCWGG 3 cut(s) 283, 1092, 1253
EcoRV GATATC 2 cut(s) 835, 920
EcoT22I ATGCAT 1 cut(s) 827
EcoT38I GRGCYC 1 cut(s) 244
FaeI CATG 4 cut(s) 412, 825, 1192, 1266
FalI AAGNNNNNCTT 4 cut(s) 706, 738, 1026, 1058
FatI CATG 4 cut(s) 408, 821, 1188, 1262
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 996
FbaI TGATCA 1 cut(s) 1180
Fnu4HI GCNGC 4 cut(s) 102, 209, 768, 1362
FokI GGATG 2 cut(s) 635, 1420
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 4 cut(s) 102, 209, 768, 1362
FspBI CTAG 1 cut(s) 1116
GluI GCNGC 4 cut(s) 102, 209, 768, 1362
GsuI CTGGAG 2 cut(s) 548, 1276
HaeIII GGCC 3 cut(s) 282, 1155, 1353
HapII CCGG 4 cut(s) 137, 147, 174, 326
HgaI GACGC 2 cut(s) 827, 1036
Hin1I GRCGYC 2 cut(s) 402, 1047
Hin1II CATG 4 cut(s) 412, 825, 1192, 1266
HindIII AAGCTT 1 cut(s) 1314
HpaII CCGG 4 cut(s) 137, 147, 174, 326
HphI GGTGA 5 cut(s) 161, 203, 584, 1190, 1193
Hpy166II GTNNAC 4 cut(s) 799, 944, 1101, 1293
Hpy188I TCNGA 7 cut(s) 235, 751, 832, 937, 1052, 1167, 1245
Hpy188III TCNNGA 5 cut(s) 174, 374, 1034, 1106, 1184
Hpy8I GTNNAC 4 cut(s) 799, 944, 1101, 1293
HpyAV CCTTC 5 cut(s) 164, 589, 896, 932, 1047
HpyCH4III ACNGT 5 cut(s) 60, 759, 1082, 1175, 1339
HpyCH4IV ACGT 6 cut(s) 402, 795, 801, 879, 1159, 1328
HpyF10VI GCNNNNNNNGC 4 cut(s) 288, 523, 539, 848
HpyF3I CTNAG 3 cut(s) 234, 947, 1413
HpySE526I ACGT 6 cut(s) 402, 795, 801, 879, 1159, 1328
Hsp92I GRCGYC 2 cut(s) 402, 1047
Hsp92II CATG 4 cut(s) 412, 825, 1192, 1266
Kpn2I TCCGGA 1 cut(s) 173
KpnI GGTACC 1 cut(s) 29
Ksp22I TGATCA 1 cut(s) 1180
Kzo9I GATC 4 cut(s) 253, 751, 1167, 1180
LmnI GCTCC 2 cut(s) 247, 357
Lsp1109I GCAGC 3 cut(s) 88, 220, 1348
LweI GCATC 5 cut(s) 412, 694, 829, 838, 1027
MaeI CTAG 1 cut(s) 1116
MaeII ACGT 6 cut(s) 402, 795, 801, 879, 1159, 1328
MaeIII GTNAC 6 cut(s) 149, 191, 328, 572, 685, 1111
MalI GATC 4 cut(s) 255, 753, 1169, 1182
MboI GATC 4 cut(s) 253, 751, 1167, 1180
MboII GAAGA 5 cut(s) 44, 212, 301, 927, 1121
MfeI CAATTG 1 cut(s) 1009
MhlI GDGCHC 3 cut(s) 244, 1061, 1165
MluCI AATT 8 cut(s) 346, 509, 672, 717, 1009, 1084, 1194, 1321
MlyI GAGTC 3 cut(s) 239, 569, 1234
MnlI CCTC 9 cut(s) 267, 272, 523, 657, 1141, 1209, 1251, 1340, 1343
Mph1103I ATGCAT 1 cut(s) 827
MroI TCCGGA 1 cut(s) 173
MseI TTAA 2 cut(s) 62, 336
MslI CAYNNNNRTG 5 cut(s) 388, 593, 976, 1026, 1216
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 4 cut(s) 137, 147, 174, 326
MspR9I CCNGG 4 cut(s) 138, 285, 1094, 1255
MunI CAATTG 1 cut(s) 1009
Mva1269I GAATGC 1 cut(s) 1388
MvaI CCWGG 3 cut(s) 285, 1094, 1255
MwoI GCNNNNNNNGC 4 cut(s) 288, 523, 539, 848
NciI CCSGG 1 cut(s) 138
NdeI CATATG 1 cut(s) 996
NdeII GATC 4 cut(s) 253, 751, 1167, 1180
NlaIII CATG 4 cut(s) 412, 825, 1192, 1266
NlaIV GGNNCC 4 cut(s) 27, 359, 680, 888
NmuCI GTSAC 4 cut(s) 149, 191, 572, 1111
NsiI ATGCAT 1 cut(s) 827
NspI RCATGY 1 cut(s) 825
OliI CACNNNNGTG 1 cut(s) 1026
PaeI GCATGC 1 cut(s) 825
PctI GAATGC 1 cut(s) 1388
PfeI GAWTC 7 cut(s) 130, 170, 306, 441, 655, 692, 806
PkrI GCNGC 4 cut(s) 103, 210, 769, 1363
PleI GAGTC 3 cut(s) 238, 569, 1234
PpsI GAGTC 3 cut(s) 238, 569, 1234
Ppu21I YACGTR 2 cut(s) 796, 1160
PsiI TTATAA 1 cut(s) 990
Psp124BI GAGCTC 1 cut(s) 244
Psp6I CCWGG 3 cut(s) 283, 1092, 1253
PspEI GGTNACC 1 cut(s) 149
PspGI CCWGG 3 cut(s) 283, 1092, 1253
PspN4I GGNNCC 4 cut(s) 27, 359, 680, 888
PspPI GGNCC 3 cut(s) 281, 323, 965
PstI CTGCAG 4 cut(s) 250, 519, 1288, 1366
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 6 cut(s) 27, 558, 1100, 1292, 1370, 1418
RsaNI GTAC 6 cut(s) 26, 557, 1099, 1291, 1369, 1417
RseI CAYNNNNRTG 5 cut(s) 388, 593, 976, 1026, 1216
SacI GAGCTC 1 cut(s) 244
SaqAI TTAA 2 cut(s) 62, 336
SatI GCNGC 4 cut(s) 102, 209, 768, 1362
Sau3AI GATC 4 cut(s) 253, 751, 1167, 1180
Sau96I GGNCC 3 cut(s) 281, 323, 965
SchI GAGTC 3 cut(s) 239, 569, 1234
ScrFI CCNGG 4 cut(s) 138, 285, 1094, 1255
SduI GDGCHC 3 cut(s) 244, 1061, 1165
SfaNI GCATC 5 cut(s) 412, 694, 829, 838, 1027
SfcI CTRYAG 5 cut(s) 246, 515, 1171, 1284, 1362
SinI GGWCC 2 cut(s) 323, 965
SmiMI CAYNNNNRTG 5 cut(s) 388, 593, 976, 1026, 1216
SmlI CTYRAG 2 cut(s) 659, 1104
SmoI CTYRAG 2 cut(s) 659, 1104
SnaBI TACGTA 1 cut(s) 796
SpeI ACTAGT 1 cut(s) 1115
SphI GCATGC 1 cut(s) 825
Sse9I AATT 8 cut(s) 346, 509, 672, 717, 1009, 1084, 1194, 1321
SsiI CCGC 2 cut(s) 30, 767
SspI AATATT 1 cut(s) 618
SspMI CTAG 1 cut(s) 1116
SstI GAGCTC 1 cut(s) 244
StyD4I CCNGG 4 cut(s) 136, 283, 1092, 1253
TaaI ACNGT 5 cut(s) 60, 759, 1082, 1175, 1339
TaiI ACGT 6 cut(s) 405, 798, 804, 882, 1162, 1331
TaqI TCGA 2 cut(s) 388, 837
TasI AATT 8 cut(s) 346, 509, 672, 717, 1009, 1084, 1194, 1321
TatI WGTACW 2 cut(s) 556, 1368
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 7 cut(s) 130, 170, 306, 441, 655, 692, 806
Tru1I TTAA 2 cut(s) 62, 336
Tru9I TTAA 2 cut(s) 62, 336
TscAI CASTG 5 cut(s) 171, 790, 897, 913, 1180
TseFI GTSAC 4 cut(s) 149, 191, 572, 1111
TseI GCWGC 3 cut(s) 101, 208, 1361
Tsp45I GTSAC 4 cut(s) 149, 191, 572, 1111
TspGWI ACGGA 2 cut(s) 704, 1160
TspRI CASTG 5 cut(s) 171, 790, 897, 913, 1180
VpaK11BI GGWCC 2 cut(s) 323, 965
XapI RAATTY 2 cut(s) 509, 717
XceI RCATGY 1 cut(s) 825
XspI CTAG 1 cut(s) 1116
ZraI GACGTC 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.