Rh1BG213300

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000839
Physical Location & Seq
Forward (+)
33229546 .. 33254630
25085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG213300.1

Sequence Viewer

Length: 768 bp
ATGTACTCACCTCGGTTGGCTTATAGATGCCAGGTGCTGGGGAGTGTTTCTGTACATATCTCGATACTTTGGACAGGTCCAGTAACTTTTAAGTTTGAAAATTGCAATGGGAGCCTCCCAGCAGTTCTTGACAATCCATTTCGATGGACAAAGGACGTCAGCATGATATACATAGATGCACCTGTTGGTGCTGGATTCTCCTACTCAAAAACTCAAAGCGGTTATGTTATGGATGACTATAAATATGTTGCACAAAGCTATGAATTTCTGCAGAAGTGGCTGGACGCACACCCTGCATATTTGGAAAATATACTCTACATTGGTGGTGACTCATTCTCTGGCATAATTGTGCCAATGATTGTACAACAAATATTGGATGGTAATGAAAATGGAACTTTGCCTCTAATGAATCTCAAGGGATATGTAATTGGGAACCCAGTAACGGATTCATTCATTGATGCGAATGCAAGAATTCCTTATGCCCATCGGCTAACTCTTATATCAGATCAACTGTATCAGGCGGCGAAAACAAGTTGCAGTGGAGATTACGTAAACGTACATTCAAGCAATGACGCATGCATTTCAGACATCGATGCTATTGATGCAATTGCCCCGATAGGTGTAGGTATTCACTGGCATCCAGATGATTTCCATGTGGCTTTGTTTCCTTGCTCCATGAAGAGAGAGAACAATCCAGAGAGAAATCAAGAACGATCGAGACTTCGAGAGAGTGCAAGTGTGTTTCTTTGCTTTGCCCGAAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

255

Amino Acids

28.55

Weight (kDa)

5.46

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 25 - 203 3.1e-49 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 159
AccB7I CCANNNNNTGG 1 cut(s) 37
AciI CCGC 2 cut(s) 219, 521
AcsI RAATTY 2 cut(s) 263, 471
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 4 cut(s) 5, 54, 363, 558
AfiI CCNNNNNNNGG 2 cut(s) 37, 442
AgsI TTSAA 2 cut(s) 98, 564
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 1 cut(s) 258
AluI AGCT 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 712
AlwNI CAGNNNCTG 1 cut(s) 37
ApoI RAATTY 2 cut(s) 263, 471
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 338
AvaII GGWCC 1 cut(s) 77
BccI CCATC 3 cut(s) 138, 371, 492
BciT130I CCWGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 712
BfmI CTRYAG 1 cut(s) 269
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 2 cut(s) 113, 434
BmrFI CCNGG 1 cut(s) 32
BmrI ACTGGG 1 cut(s) 431
BmsI GCATC 6 cut(s) 17, 166, 448, 583, 592, 646
BmuI ACTGGG 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 591
BsaAI YACGTR 1 cut(s) 550
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 534, 564
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 442
Bse1I ACTGG 3 cut(s) 80, 437, 638
Bse3DI GCAATG 2 cut(s) 112, 574
BseBI CCWGG 1 cut(s) 32
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 3 cut(s) 238, 382, 637
BseLI CCNNNNNNNGG 2 cut(s) 37, 442
BseMI GCAATG 2 cut(s) 112, 574
BseNI ACTGG 3 cut(s) 80, 437, 638
BseYI CCCAGC 2 cut(s) 37, 118
Bsh1285I CGRYCG 1 cut(s) 716
BshVI ATCGAT 1 cut(s) 591
BsiEI CGRYCG 1 cut(s) 716
BslI CCNNNNNNNGG 2 cut(s) 37, 442
BsmAI GTCTC 1 cut(s) 712
BsmI GAATGC 1 cut(s) 469
Bsp1407I TGTACA 2 cut(s) 52, 361
Bsp143I GATC 2 cut(s) 505, 713
BspACI CCGC 2 cut(s) 219, 521
BspDI ATCGAT 1 cut(s) 591
BspLI GGNNCC 2 cut(s) 113, 434
BspMAI CTGCAG 1 cut(s) 273
BsrDI GCAATG 2 cut(s) 112, 574
BsrGI TGTACA 2 cut(s) 52, 361
BsrI ACTGG 3 cut(s) 80, 437, 638
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 2 cut(s) 505, 713
BssNI GRCGYC 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 513
Bst6I CTCTTC 2 cut(s) 674, 754
BstACI GRCGYC 1 cut(s) 156
BstAPI GCANNNNNTGC 1 cut(s) 293
BstAUI TGTACA 2 cut(s) 52, 361
BstBAI YACGTR 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 577
BstF5I GGATG 3 cut(s) 238, 382, 637
BstKTI GATC 2 cut(s) 508, 716
BstMAI GTCTC 1 cut(s) 712
BstMBI GATC 2 cut(s) 505, 713
BstMCI CGRYCG 1 cut(s) 716
BstMWI GCNNNNNNNGC 4 cut(s) 111, 277, 293, 602
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 269
BstSNI TACGTA 1 cut(s) 550
BstXI CCANNNNNNTGG 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 591
BsuTUI ATCGAT 1 cut(s) 591
BtsCI GGATG 3 cut(s) 238, 382, 637
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 544, 631
Cac8I GCNNGC 1 cut(s) 577
CaiI CAGNNNCTG 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 591
CseI GACGC 2 cut(s) 293, 581
Csp6I GTAC 4 cut(s) 4, 53, 362, 557
CviAII CATG 4 cut(s) 163, 576, 653, 676
CviJI RGCY 6 cut(s) 20, 114, 258, 280, 490, 659
CviKI_1 RGCY 6 cut(s) 20, 114, 258, 280, 490, 659
CviQI GTAC 4 cut(s) 4, 53, 362, 557
DpnI GATC 2 cut(s) 507, 715
DpnII GATC 2 cut(s) 505, 713
Eam1104I CTCTTC 2 cut(s) 674, 754
EarI CTCTTC 2 cut(s) 674, 754
Eco105I TACGTA 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 581
FaeI CATG 4 cut(s) 166, 579, 656, 679
FalI AAGNNNNNCTT 2 cut(s) 460, 492
FatI CATG 4 cut(s) 162, 575, 652, 675
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 3 cut(s) 245, 389, 624
Fsp4HI GCNGC 1 cut(s) 522
GluI GCNGC 1 cut(s) 522
GsaI CCCAGC 2 cut(s) 41, 122
HgaI GACGC 2 cut(s) 293, 581
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 4 cut(s) 166, 579, 656, 679
HinfI GANTC 4 cut(s) 195, 329, 409, 446
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 2 cut(s) 505, 586
Hpy188III TCNNGA 7 cut(s) 61, 128, 641, 695, 707, 717, 725
Hpy8I GTNNAC 1 cut(s) 553
HpyCH4III ACNGT 1 cut(s) 513
HpyCH4IV ACGT 3 cut(s) 156, 549, 555
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 277, 293, 602
HpySE526I ACGT 3 cut(s) 156, 549, 555
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 4 cut(s) 166, 579, 656, 679
Kzo9I GATC 2 cut(s) 505, 713
LmnI GCTCC 2 cut(s) 111, 677
LweI GCATC 6 cut(s) 17, 166, 448, 583, 592, 646
MaeII ACGT 3 cut(s) 156, 549, 555
MaeIII GTNAC 3 cut(s) 82, 326, 439
MalI GATC 2 cut(s) 507, 715
MboI GATC 2 cut(s) 505, 713
MboII GAAGA 1 cut(s) 691
MfeI CAATTG 1 cut(s) 606
MluCI AATT 6 cut(s) 100, 263, 345, 426, 471, 606
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 3 cut(s) 21, 125, 411
Mph1103I ATGCAT 1 cut(s) 581
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 3 cut(s) 142, 347, 642
MspR9I CCNGG 1 cut(s) 32
MunI CAATTG 1 cut(s) 606
Mva1269I GAATGC 1 cut(s) 469
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 4 cut(s) 111, 277, 293, 602
NdeII GATC 2 cut(s) 505, 713
NlaIII CATG 4 cut(s) 166, 579, 656, 679
NlaIV GGNNCC 2 cut(s) 113, 434
NmuCI GTSAC 1 cut(s) 326
NsiI ATGCAT 1 cut(s) 581
NspI RCATGY 1 cut(s) 579
PaeI GCATGC 1 cut(s) 579
PctI GAATGC 1 cut(s) 469
PfeI GAWTC 3 cut(s) 195, 409, 446
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 1 cut(s) 523
Ple19I CGATCG 1 cut(s) 716
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
Ppu21I YACGTR 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 30
PspFI CCCAGC 2 cut(s) 37, 118
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 113, 434
PspPI GGNCC 1 cut(s) 77
PstI CTGCAG 1 cut(s) 273
PstNI CAGNNNCTG 1 cut(s) 37
PvuI CGATCG 1 cut(s) 716
RsaI GTAC 4 cut(s) 5, 54, 363, 558
RsaNI GTAC 4 cut(s) 4, 53, 362, 557
RseI CAYNNNNRTG 3 cut(s) 142, 347, 642
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 2 cut(s) 505, 713
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 32
SfaNI GCATC 6 cut(s) 17, 166, 448, 583, 592, 646
SfcI CTRYAG 1 cut(s) 269
SinI GGWCC 1 cut(s) 77
SmiMI CAYNNNNRTG 3 cut(s) 142, 347, 642
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SnaBI TACGTA 1 cut(s) 550
SphI GCATGC 1 cut(s) 579
Sse9I AATT 6 cut(s) 100, 263, 345, 426, 471, 606
SsiI CCGC 2 cut(s) 219, 521
SspI AATATT 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 1 cut(s) 513
TaiI ACGT 3 cut(s) 159, 552, 558
TaqI TCGA 5 cut(s) 62, 142, 591, 716, 724
TasI AATT 6 cut(s) 100, 263, 345, 426, 471, 606
TatI WGTACW 3 cut(s) 3, 52, 361
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 3 cut(s) 195, 409, 446
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 2 cut(s) 544, 638
TseFI GTSAC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 6 cut(s) 276, 399, 422, 438, 442, 692
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 2 cut(s) 544, 638
Van91I CCANNNNNTGG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 77
XapI RAATTY 2 cut(s) 263, 471
XceI RCATGY 1 cut(s) 579
ZraI GACGTC 1 cut(s) 157
Zsp2I ATGCAT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.